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Trabajo presentado al Congreso de la DES de Ciencias de la Universidad Autónoma de San Luis Potosí, celebrado del 11 al 15 de octubre de 2021.

Resumen del trabajo presentado al APS March Meeting, celebrado de forma virtual del 13 al 19 de marzo de 2021., We investigatenanotubes of two-dimensional materials isoelectronic to phosphorene by rolling up the recently discovered layers of silicon monosulfide, in phasesthat in order of decreasing stability are labeled as P mma and β [1-3]. We find that the nanotubes of thick layer P mma silicon monosulfide grownaround 1 nm in diameter are stable at room temperature. The nanotubes having small diameters show metallic character, and with increasingdiameter, they become semiconductors because gaps are open by the dimerization of the silicon-silicon distances. Lying at higher energy, we alsofind that the β SiS monolayer nanotubes are stable with negative strain energy, similar to imogolite nanotubes. The theoretical thermal stability ofthese two types of silicon monosulfide nanotubes showing such intriguing properties would request further to investigate procedures for theirnovel synthesis by experimentalists., Project PID2019-105488GB-I00 of the Spanish MICINN, and Project No. IT1246-19 of the Gobierno Vasco-UPV/EHU.

The development of new molecules for the treatment of leishmaniasis is, a neglected parasitic disease, is urgent as current anti-leishmanial therapeutics are hampered by drug toxicity and resistance. The pyrrolo[1,2-b]isoquinoline core was selected as starting point, and palladium-catalyzed Heck-initiated cascade reactions were developed for the synthesis of a series of C-10 substituted derivatives. Their in vitro leishmanicidal activity against visceral (L. donovani) and cutaneous (L. amazonensis) leishmaniasis was evaluated. The best activity was found, in general, for the 10-arylmethyl substituted pyrroloisoquinolines. In particular, 2ad (IC50 = 3.30 μM, SI > 77.01) and 2bb (IC50 = 3.93 μM, SI > 58.77) were approximately 10-fold more potent and selective than the drug of reference (miltefosine), against L. amazonensis on in vitro promastigote assays, while 2ae was the more active compound in the in vitro amastigote assays (IC50 = 33.59 μM, SI > 8.93). Notably, almost all compounds showed low cytotoxicity, CC50 > 100 μg/mL in J774 cells, highest tested dose. In addition, we have developed the first Perturbation Theory Machine Learning (PTML) algorithm able to predict simultaneously multiple biological activity parameters (IC50, Ki, etc.) vs. any Leishmania species and target protein, with high values of specificity (>98%) and sensitivity (>90%) in both training and validation series. Therefore, this model may be useful to reduce time and assay costs (material and human resources) in the drug discovery process., Ministerio de Economía y Competitividad (CTQ2016-74881-P), Ministerio de Ciencia e Innovación (PID2019-104148 GB-I00) and Gobierno Vasco (IT1045-16) are gratefully acknowledged for their financial support. I. B. wishes to thank Fundación Biofísica Bizkaia/Biofisika Bizkaia Fundazioa (FBB) for a postdoctoral grant.

Resumen del trabajo presentado a la conferencia Graphene, celebrada en Grenoble (Francia) del 26 al 29 de octubre de 2021.

Resumen del trabajo presentado al ImagineNano, celebrado en Bilbao (España) del 23 al 24 de noviembre de 2021.

Graphene is a two-dimensional material, with exceptional mechanical, electrical, and thermal properties. Graphene-based materials are, therefore, excellent candidates for use in nanocomposites. We investigated reduced graphene oxide (rGO), which is produced easily by oxidizing and exfoliating graphite in calcium silicate hydrate (CSHs) composites, for use in cementitious materials. The density functional theory was used to study the binding of moieties, on the rGO surface (e.g., hydroxyl-OH/rGO and epoxide/rGO groups), to CSH units, such as silicate tetrahedra, calcium ions, and OH groups. The simulations indicate complex interactions between OH/rGO and silicate tetrahedra, involving condensation reactions and selective repairing of the rGO lattice to reform pristine graphene. The condensation reactions even occurred in the presence of calcium ions and hydroxyl groups. In contrast, rGO/CSH interactions remained close to the initial structural models of the epoxy rGO surface. The simulations indicate that specific CSHs, containing rGO with different interfacial topologies, can be manufactured using coatings of either epoxide or hydroxyl groups. The results fill a knowledge gap, by establishing a connection between the chemical compositions of CSH units and rGO, and confirm that a wet chemical method can be used to produce pristine graphene by removing hydroxyl defects from rGO., This research was funded by Karlsruhe House of Young Science (KHYS), and Deutsche Forschungsgemeinschaft (DFG). A.A. and J.S.D. also acknowledge funding from the Spanish Ministry of Science and Innovation (grants nos. PID2019-105488GB-I00 and PCI2019-103657 grant), the Gobierno Vasco UPV/EHU (project no. IT-1246-19), and the European Commission NRG-STORAGE project (project no. GA 870114). Research conducted in the scope of the Transnational Common Laboratory (LTC) Aquitaine-Euskadi Network in Green Concrete and Cement-based Materials.

arXiv:2006.10837v2, Generating and detecting radiation in the technologically relevant range of the so-called terahertz gap (0.1–10 THz) is challenging because of a lack of efficient sources and detectors. Quantum dots in carbon nanotubes have shown great potential to build sensitive terahertz detectors, usually based on photon-assisted tunneling. A recently reported mechanism combining resonant quantum dot transitions and tunneling barrier asymmetries results in a narrow linewidth photocurrent response with a large signal-to-noise ratio under weak THz radiation. That device was sensitive to one frequency, corresponding to transitions between equidistant quantized states. In this work we show, using numerical simulations together with scanning tunneling spectroscopy studies of a defect-induced metallic zigzag single-walled carbon nanotube quantum dot, that breaking simultaneously various symmetries in metallic nanotube quantum dots of arbitrary chirality strongly relaxes the selection rules in the electric dipole approximation and removes energy degeneracies. This leads to a richer set of allowed optical transitions spanning frequencies from 1 THz to several tens of THz, for a ∼10 nm quantum dot. Based on these findings, we propose a terahertz detector device based on a metallic single-walled carbon nanotube quantum dot defined by artificial defects. Depending on its length and contacts transparency, the operating regimes range from a high-resolution gate-tunable terahertz sensor to a broadband terahertz detector. Our calculations indicate that the device is largely unaffected by temperatures up to 100 K, making carbon nanotube quantum dots with broken symmetries a promising platform to design tunable terahertz detectors that could operate at liquid nitrogen temperatures., This work has been partially supported by the Spanish Ministry of Science and Innovation with PID2019-105488GB-I00 and PCI2019-103657 (A.A.) and FIS2017-82804-P (D.B.). The work of D.B. is partially supported by by the Transnational Common Laboratory QuantumChemPhys. The Basque Government supported this work through Project No. IT-1246-19 (A.A.). J.W.G. acknowledges financial support from FONDECYT: Iniciación en Investigación 2019 Grant N. 11190934 (Chile). A.A. acknowledge financial support by the European Commission from the NRG-STORAGE project (GA 870114). K.E., C.P. and D.P. acknowledge the Swiss National Science Foundation under Grant No. 200020_182015 and No. 200021_172527, and the NCCR MARVEL funded by the Swiss National Science Foundation (51NF40-182892). The Swiss National Supercomputing Centre (CSCS) under project ID s746 and s904 is acknowledged for computational resources.

Resumen del trabajo presentado a la conferencia Graphene, celebrada en Grenoble (Francia) del 26 al 29 de octubre de 2021.

Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/2304-8158/9/12/1913 Este artículo pertenece al número especial "Reviews on food microbiology, foodborne pathogens, and probiotics". In recent decades, worldwide fish consumption has increased notably worldwide. Despite the health benefits of fish consumption, it also can suppose a risk because of fishborne diseases, including parasitic infections. Global changes are leading to the emergence of parasites in new locations and to the appearance of new sources of transmission. That is the case of the zoonotic protozoa Cryptosporidium spp., Giardia duodenalis, and Toxoplasma gondii; all of them reach aquatic environments and have been found in shellfish. Similarly, these protozoa can be present in other aquatic animals, such as fish. The present review gives an overview on these three zoonotic protozoa in order to understand their potential presence in fish and to comprehensively revise all the evidences of fish as a new potential source of Cryptosporidium spp., Giardia duodenalis, and Toxoplasma gondii transmission. All of them have been found in both marine and freshwater fishes. Until now, it has not been possible to demonstrate that fish are natural hosts for these protozoa; otherwise, they would merely act as mechanical transporters. Nevertheless, even if fish only accumulate and transport these protozoa, they could be a “new” source of infection for people.

Amphotericin B (AmB) is a high-molecular weight poorly soluble drug with a high efficacy in the treatment of infectious caused by Leishmania spp. parasites, which possesses a very low topical bioavailability. Transferosomes (TFs) are ultradeformable vesicles that consist of drugs lipids, an edge activator, and a low amount of ethanol (

The clinical picture of SARS-CoV-2 infection (COVID-19) is characterized in its more severe form, by an acute respiratory failure which can worsen to pneumonia and acute respiratory distress syndrome (ARDS), and get complicated with thrombotic events and heart dysfunction. Therefore, admission to the Intensive Care Unit (ICU) is common. Ultrasound, which has become an everyday tool in the ICU, can be very useful during COVID-19 pandemic, since it provides the clinician with information which can be interpreted and integrated within a global assessment during the physical examination. A description of some of the potential applications of ultrasound is depicted in this document, in order to supply the physicians taking care of these patients with a adapted guide to the intensive care setting. Some of its applications since ICU admission include verification of the correct position of the endotracheal tube, contribution to safe cannulation of lines, and identification of complications and thrombotic events. Furthermore, pleural and lung ultrasound can be an alternative diagnostic test to assess the degree of involvement of the lung parenchyma by means of the evaluation of specific ultrasound patterns, identification of pleural effusions and barotrauma. Echocardiography provides information of heart involvement, detects cor pulmonale and shock states.

Este artículo se encuentra disponible en la siguiente URL: https://pubs.acs.org/doi/10.1021/acsinfecdis.0c00293 Nota de Fondos: Esta versión preprint del artículo se encuentra a texto completo en el CEU Repositorio Institucional. En esta investigación también participan: Francisco Bolás-Fernández, Maria Paloma Ballesteros, Marcia Dalastra Laurenti, Luiz F. D. Passero, Aikaterini Lalatsa y Dolores R. Serrano. This is the pre-peer reviewed version of the following article: Fernández-García, R., Statts, L., Jesús, J.A., Dea-Ayuela, M.A., Bautista, L., Simao, R. et al. (2020). Ultradeformable lipid vesicles localize Amphotericin B in the dermis for the treatment of infectious skin diseases. ACS Infectious Diseases, vol. 6, i. 10 (18 aug.), pp. 2647-2660, which has been published in final form at https://doi.org/10.1021/acsinfecdis.0c00293 Este es el pre-print del siguiente artículo: Fernández-García, R., Statts, L., Jesús, J.A., Dea-Ayuela, M.A., Bautista, L., Simao, R. et al. (2020). Ultradeformable lipid vesicles localize Amphotericin B in the dermis for the treatment of infectious skin diseases. ACS Infectious Diseases, vol. 6, i. 10 (18 aug.), pp. 2647-2660, que se ha publicado de forma definitiva en https://doi.org/10.1021/acsinfecdis.0c00293 Cutaneous fungal and parasitic diseases remain challenging to treat, as available therapies are unable to permeate the skin barrier. Thus, treatment options rely on systemic therapy, which fail to produce high drug local concentrations but can lead to significant systemic toxicity. Amphotericin B (AmB) is highly efficacious in the treatment of both fungal and parasitic diseases such as cutaneous leishmaniasis, but is only reserved for parenteral administration in patients with severe pathophysiology. Here, we have designed and optimised AmB-transfersomes [93.5 % encapsulation efficiency, size of 150 nm, and good colloidal stability (-35.02 mV)] that can remain physicochemically stable (>90 % drug content) at room temperature and 4 °C over 6 months when lyophilised and stored under desiccated conditions. AmBtransfersomes possessed good permeability across mouse skin (4.91 ± 0.41 μg/cm2/h) and 10-fold higher permeability across synthetic Strat-M® membranes. In vivo studies after a single topical application in mice showed permeability and accumulation within the dermis (>25 μg AmB /g skin at 6 h post-administration) indicating the delivery of therapeutic amounts of AmB for mycoses and cutaneous leishmaniasis, while a single daily administration in Leishmania (Leishmania) amazonensis infected mice over 10 days resulted in excellent efficacy (98 % reduction in Leishmania parasites). Combining the application of AmB-transfersomes with metallic microneedles in vivo increased levels in the SC and dermis but is unlikely to elicit transdermal levels. In conclusion, AmB-transfersomes are promising and stable topical nanomedicines that can be readily translated for parasitic and fungal infectious diseases.

RESUMEN La infección por SARS-CoV-2 (COVID-19) se caracteriza por producir en las formas graves, un cuadro de insuficiencia respiratoria que puede evolucionar hacia neumonía y síndrome de distrés respiratorio agudo (SDRA), presentar complicaciones como fenómenos trombóticos y disfunción cardiaca, lo que motiva el ingreso en la Unidad de Cuidados Intensivos (UCI). La ecografía, convertida en una herramienta de uso habitual en la UCI, puede ser muy útil durante la pandemia COVID-19 ya que la información obtenida por el clínico puede ser interpretada e integrada en la valoración global durante la exploración del paciente. Este documento describe algunas de sus aplicaciones con el objetivo de proporcionar una guía a los médicos responsables adaptado al paciente crítico con COVID-19. Alguna de sus aplicaciones desde el ingreso en la UCI incluyen confirmar la correcta posición del tubo endotraqueal, facilitar la inserción segura de las vías, e identificar complicaciones y fenómenos trombóticos. Además, la ecografía pleuropulmonar puede ser una alternativa diagnóstica válida que permite evaluar el grado de afectación pulmonar, mediante el análisis de patrones ecográficos específicos, identificación del derrame pleural y del barotrauma. La ecocardiografía proporciona información acerca de la afectación cardíaca, detección del cor pulmonale y estados de shock.

Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1420-3049/25/6/1394 Este artículo pertenece al número especial "Bioproducts for health". Leishmaniasis a ects around 12 million people worldwide and is estimated to cause the ninth-largest disease burden. There are three main forms of the disease, visceral (VL), cutaneous (CL), and mucocutaneous (MCL), leading to more than one million new cases every year and several thousand deaths. Current treatments based on chemically synthesized molecules are far from ideal. In this study, we have tested the in vitro and in vivo e cacy of ursolic acid (UA), a multifunctional triterpenoid with well-known antitumoral, antioxidant, and antimicrobial e ects on di erent Leishmania strains. The in vitro antileishmanial activity against the intracellular forms was six and three-fold higher compared to extracellular forms of L. amazonensis and L. infantum, respectively. UA also showed to be a potent antileishmanial drug against both VL and CL manifestations of the disease in experimental models. UA parenterally administered at 5 mg/kg for seven days significantly reduced the parasite burden in liver and spleen not only in murine acute infection but also in a chronic-infection model against L. infantum. In addition, UA ointment (0.2%) topically administered for four weeks diminished (50%) lesion size progression in a chronic infection model of CL caused by L. amazonensis, which was much greater than the e ect of UA formulated as an O/W emulsion. UA played a key role in the immunological response modulating the Th1 response. The exposure of Leishmania-infected macrophages to UA led to a significant di erent production in the cytokine levels depending on the Leishmania strain causing the infection. In conclusion, UA can be a promising therapy against both CL and VL.

Este artículo se encuentra disponible en la siguiente URL: https://www.ejfa.me/index.php/journal/article/view/2184 Tiger nut (Cyperus esculentus L.), also known as chufa (European sedge), is a member of the Cyperaceae family, which is used in organic and conventional agriculture for its small edible tubers and grown in temperate and tropical zones of the world being consumed raw, roasted or pressed for its juice as beverage. The aim of this study is analyzing the proximate composition (AOAC methods), total phenolic content (Folin-Ciocalteu method), total antioxidant capacity (Trolox equivalent antioxidant capacity, TEAC) and microbiological profile (International Standard Organization norms, ISO) of samples of Spanish organic and conventional, Nigerian conventional and unknown origin tiger nuts obtained from supermarkets and local markets in Spain. No significant differences in proximate composition and antioxidant properties were found between Spanish organic and conventional tiger-nuts, except in total phenolic level (p

Resonances sustained by plasmonic nanoparticles provide extreme electric field confinement and enhancement into the deep subwavelength domain for a plethora of applications. Recent progress in nanofabrication made it even possible to tailor the properties of nanoparticles consisting of only a few hundred atoms. These nanoparticles support both single-particle-like resonances and collective plasmonic charge density oscillations. Prototypical systems sustaining both features are graphene nanoantennas. In pushing the frontier of nanoscience, traditional identification, and classification of such resonances is at stake again. We show that in such nanostructures, the concerted electron cloud oscillation in real space does not necessarily come along with collective dynamics of conduction band electrons in energy space. This unveils an urgent need for a discussion of how a plasmon in nanostructures should be defined. Here, we propose to define it relying on energy space dynamics. The unambiguous identification of the plasmonic nature of a resonance is crucial to find out whether desirable plasmon-assisted features, such as frequency conversion processes, can be expected from a resonance. We elaborate an energy-based figure of merit that classifies the nature of resonances in nanostructures, motivated by tight binding simulations with a toy model consisting of a linear chain of atoms. We apply afterward the proposed figure of merit to a doped hexagonal graphene nanoantenna, which is known to support plasmons in the near infrared and single-particle-like transitions in the visible., M.M.M. acknowledges financial support through the Research Travel Grant by the Karlsruhe House of Young Scientists (KHYS). M.M.M. and C.R. acknowledge support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—project number 378579271—within project RO 3640/8-1 and from the VolkswagenStiftung. M.M.M. is grateful for the support of the Toruń Astrophysics/Physics Summer Program TAPS 2019 and the PROM project no. PPI/PRO/2018/1/00016/U/001 by the Polish National Agency for Academic Exchange. M.M.M., M.K., and K.S. acknowledge the hospitality of the Donostia International Physics Center. M.K. acknowledges the financial support from the Foundation for Polish Science (FNP) (project First Team/2017-3/20 cofinanced by the European Union under the European Regional Development Fund). M.P. and A.A. acknowledge financial support by project PID2019-105488GB-I00 of the Spanish Ministry of Science and Innovation, and the Gobierno Vasco—UPV/EHU project IT1246-19. K.S. acknowledges support from the National Science Centre, Poland (project no. 2016/23/G/ST3/0404).

The current therapies of leishmaniasis, the second most widespread neglected tropical disease, have limited effectiveness and toxic side effects. In this regard, natural products play an important role in overcoming the current need for new leishmanicidal agents. The present study reports a bioassay-guided fractionation of the ethanolic extract of leaves of Piper pseudoarboreum against four species of Leishmania spp. promastigote forms, which afforded six known alkamides (1&ndash, 6). Their structures were established on the basis of spectroscopic and spectrometric analysis. Compounds 2 and 3 were identified as the most promising ones, displaying higher potency against Leishmania spp. promastigotes (IC50 values ranging from 1.6 to 3.8 &micro, M) and amastigotes of L. amazonensis (IC50 values ranging from 8.2 to 9.1 &micro, M) than the reference drug, miltefosine. The efficacy of (E)-piplartine (3) against L. amazonensis infection in an in vivo model for cutaneous leishmaniasis was evidenced by a significant reduction of the lesion size footpad and spleen parasite burden, similar to those of glucantime used as the reference drug. This study reinforces the therapeutic potential of (E)-piplartine as a promising lead compound against neglected infectious diseases caused by Leishmania parasites.

La rehabilitación oclusal de pacientes parcialmente edéntulos es un desafío para los especialistas dentales en la práctica diaria. El tratamiento multidisciplinar unifica de manera coordinada todas las ramas de la odontología para alcanzar un resultado predecible a largo plazo. En este Trabajo Fin de Grado se muestras dos casos clínicos que acuden al Servicio de Prácticas Clínicas Odontológicas de la Universidad de Zaragoza durante el curso 2019-2020.

Made available in DSpace on 2020-12-12T02:25:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-15 Leishmaniasis is a neglected disease presenting cutaneous, mucosal and visceral forms and affecting an estimated 12 million mostly low-income people. Treatment of cutaneous leishmaniasis (CL) is recommended to expedite healing, reduce risk of scarring, prevent parasite dissemination to other mucocutaneous (common with New World species) or visceral forms and reduce the chance of relapse, but remains an unmet need. Available treatments are painful, prolonged (>20 days) and require hospitalisation, which increases the cost of therapy. Here we present the development of optimised topical self-nanoemulsifying drug delivery systems (SNEDDS) loaded with buparvaquone (BPQ, a hydroxynapthoquinone from the open Malaria Box) for the treatment of CL from New World species. The administration of topical BPQ-SNEDDS gels for 7 days resulted in a reduction of parasite load of 99.989 ± 0.019% similar to the decrease achieved with intralesionally administered Glucantime® (99.873 ± 0.204%) in a L. amazonensis BALB/c model. In vivo efficacy was supported by ex vivo permeability and in vivo tape stripping studies. BPQ-SNEDDS and their hydrogels demonstrated linear flux across non-infected CD-1 mouse skin ex vivo of 182.4 ± 63.0 μg cm−2 h−1 and 57.6 ± 10.8 μg cm−2 h−1 respectively localising BPQ within the skin in clinically effective concentrations (227.0 ± 45.9 μg and 103.8 ± 33.8 μg) respectively. These levels are therapeutic as BPQ-SNEDDS and their gels showed nanomolar in vitro efficacy against L. amazonensis and L. braziliensis amastigotes with excellent selectivity index toward parasites versus murine macrophages. In vivo tape stripping experiments indicated localisation of BPQ within the stratum corneum and dermis. Histology studies confirmed the reduction of parasitism and indicated healing in animals treated with BPQ-SNEDDS hydrogels. These results highlight the potential clinical capability of nano-enabled BPQ hydrogels towards a non-invasive treatment for CL. Biomaterials Bio-engineering and Nanomedicines (BioN) Laboratory Institute of Biomedical and Biomolecular Sciences School of Pharmacy and Biomedical Sciences University of Portsmouth, White Swan Road Laboratory of Pathology of Infectious Diseases (LIM-50) Medical School University of São Paulo, Avenida Dr. Arnaldo 455, 01246903 Cerqueira César Departamento de Farmacia Facultad de Ciencias de la Salud Universidad CEU Cardenal Herrera, Edificio Seminario s/n, 46113-Moncada Departament of Microbiology and Parasitology School of Pharmacy Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n Institute of Biosciences São Paulo State University (UNESP) Praça Infante Dom Henrique s/n Institute for Advanced Studies of Ocean Biosciences São Paulo State University (UNESP), Av. João Francisco Bensdorp, 1178 Departament of Pharmaceutics and Food Technology and Instituto Universitario de Farmacia Industrial (IUFI) School of Pharmacy University Complutense de Madrid, Plaza Ramón y Cajal s/n Institute of Biosciences São Paulo State University (UNESP) Praça Infante Dom Henrique s/n Institute for Advanced Studies of Ocean Biosciences São Paulo State University (UNESP), Av. João Francisco Bensdorp, 1178

Belite cements, BCs, containing mainly belite, alite and calcium aluminates, are currently used as low heat cements. These binders produce high amounts of C–S–H gel and have very good durability properties which are reviewed. Additional advantages include: (i) lower limestone demand, with lower associated CO2 emissions; (ii) lower energy demand; (iii) lower kiln operating temperature, which means lowering CO2 and NOx emissions from fuel burning; and (iv) lower temperature increase at early hydration age. However, early-age strength developments are not competitive with those of Portland cements. Hence, to improve their early-age strength developments is a research priority known as activation. This enhancement can be attained by three compatible approaches: (i) chemical, (ii) physical; and (iii) admixture activations. The current research status for BCs activation is reviewed including: cost-effective element substitutions to stabilize high-temperature forms; fast cooling, milling and mild temperature hydration as physical activation; and the use of C–S–H seeds as admixture activation. After discussion of the resulting microstructures, a research outlook is exercised., The work in Malaga has been supported by BIA2017-82391-R research grant, which is co-funded by FEDER. The work in San Sebastián was supported by PID2019-105488GB-I00 research grant, the Gobierno Vasco UPV/EHU (Project No. IT-1246-19), and the EIG CONCERT-Japan 5th Joint Call on Functional Porous Materials (Project No. PCI2019-103657-POROPCM).

Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: http://www.eurekaselect.com/166607/article This is the pre-peer reviewed version of the following article: Dea Ayuela, MA., Galiana-Roselló, C., Lalatsa, A. & Serrano, DR. (2018). Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs). Current Topics in Medicinal Chemistry, vol. 18, n. 16, pp. 1358-1374, which has been published in final form at https://doi.org/10.2174/1568026618666181025095735 Este es el post-print del siguiente artículo: Dea Ayuela, MA., Galiana-Roselló, C., Lalatsa, A. & Serrano, DR. (2018). Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs). Current Topics in Medicinal Chemistry, vol. 18, n. 16, pp. 1358-1374, que se ha publicado de forma definitiva en https://doi.org/10.2174/1568026618666181025095735 One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector-borne diseases. A quick and precise diagnosis is critical in selecting the optimal therapeutic regime that avoids unnecessary treatments and the emergence of resistance. Molecular assays based on loopmediated isothermal amplification (LAMP) techniques are a good alternative to light microscopy and antigen-based rapid diagnostic tests in developing countries, since they allow for a large amount of genetic material generated from a few copies of DNA, and use primers that lead to high sensitivity and specificity, while the amplification process can be performed in isothermal conditions without the need of sophisticated equipment to interpret the results. In this review, the main advances in the development of LAMP assays for the diagnosis of malaria, leishmaniasis and Chagas' disease are discussed as well as the feasibility of their implementation in developing countries and use as point- of-care diagnostic tests. Postprint