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Abstract A novel antibacterial immunostimulant using Platinum nanoparticles (PtNPs) and lectin from Metapenaeus dobsoni (Md-Lec) was developed. The Md-Lec and PtNPs (Pt-lec) hybrid formed through non-covalent interaction exhibits antimicrobial activity against fish specific pathogens by affecting membrane integrity and producing excess reactive oxygen species. The therapeutic efficacy of Pt-lec was demonstrated through rescuing Aeromonas hydrophila infected Nile Tilapia. Pt-lec prevents the infection spreading and reduces the bacterial bioburden in less than 12 h, and as a result of this the fish were restored to normalcy. To assess immunostimulation, we studied the expression of three different immune related genes, namely LEC, Myd88 and COX-2 in the gills, liver, spleen and kidney of fish under various experimental conditions. Our results showed that Pt-lec treatment appeared to be better when compared to lectin alone in enhancing the expression of Myd88 and COX-2, but LEC was not as expected. These results suggest that Pt-lec has the ability to protect Nile Tilapia against bacterial infection by restricting bacterial bioburden through their direct effects on the bacterial membrane and indirectly through their effects on host immune-related gene expression. This hybrid could have potential “green” application in fish farming in rescuing infected animals when compared to widely and unregulated antibiotics.

ABSTRACT: Objective: An opportunistic pathogen, Trueperella pyogenes can infect cattle, buffalo, pig, goat, cat, dog, forest musk deer, etc., affecting various organs. The aim of this study was to identify the multidrug resistance genomic islands of T. pyogenes genomes available in NCBI database and also in the recently isolated strain TN_CUL_2020. Methods: The strain TN_CUL_2020 isolated from swine lung abscess was sequenced by Illumina platform, and all the available T. pyogenes genome in NCBI database was retrieved for the comparative analysis. The ABRicate searches was used to identify antimicrobial resistance genes, and genomic islands (GIs) were predicted using IslandViewer 4. Results: The strains SH01, SH02, and TP1 were predicted with maximum number of drug resistance genes. Genomic islands identified had multidrug resistance genes along with the class I integron and/or IS6100 elements in SH01, SH02, TP1. Composite transposons of IS6100 were noted in T2849, T4479, and TP3 intercalating tet(33) resistance genes. Several strains were predicted with phage elements, type IV secretion system, the toxin-antitoxin system in the GIs. Conclusion: Swine strains SH01, SH02 were predicted with multidrug resistance genes along with class I integrons. The presence of class I integrons, insertional elements, type IV secretion system, toxin-antitoxin system, and phage elements may aid in the horizontal transfer of antimicrobial resistance genes.

Postmenopausal women are at great risk of mental health deterioration, which may lead to morbidity and mortality. The decrement of mental health with aging is attributed to hormonal changes, lowered physical activity, sleep disturbances, economic factors, as well as modifiable variables such as smoking and obesity. Studies have shown controversial results on the association between obesity and mental health in postmenopausal women. This study is a systematic review of the evidence available on the association between obesity and mental health in postmenopausal women with the aim to identify the most reliable obesity measure that has been shown in association with mental health as well as the effective measures that have been practiced for improving mental health in postmenopausal obese women. CINAHL, Scopus, Science Direct and PubMed including Medline databases were searched. Out of 3,766 articles, 23 studies of average to good quality were included, out of which 17 were cross-sectional and 6 interventional. Out of the 17 studies, 12 showed a positive association between obesity and deterioration of mental health, 3 showed a negative association and two showed no association. From the interventional studies, 4 showed positive and two not significant impact of the intervention used on obesity and mental health. In conclusion, more studies showed a positive association between obesity, especially visceral obesity, and mental health issues particularly depression, anxiety, and sleep disorders. Combination of caloric restriction and exercise seems to have a better impact on the mental health of the postmenopausal in comparison with other interventions.

The Unmanned aerial vehicles (UAVs) - based imaging is being intensively explored for precise crop evaluation. Various optical sensors, such as RGB, multi-spectral, and hyper-spectral cameras, can be used for this purpose. Consistent image quality is crucial for accurate plant trait prediction (i.e., phenotyping). However, achieving consistent image quality can pose a challenge as image qualities can be affected by i) UAV and camera technical settings, ii) environment, and iii) crop and field characters which are not always under the direct control of the UAV operator. Therefore, capturing the images requires the establishment of robust protocols to acquire images of suitable quality, and there is a lack of systematic studies on this topic in the public domain. Therefore, in this case study, we present an approach (protocols, tools, and analytics) that addressed this particular gap in our specific context. In our case, we had the drone (DJI Inspire 1 Raw) available, equipped with RGB camera (DJI Zenmuse x5), which needed to be standardized for phenotyping of the annual crops’ canopy cover (CC). To achieve this, we have taken 69 flights in Hyderabad, India, on 5 different cereal and legume crops (∼300 genotypes) in different vegetative growth stages with different combinations of technical setups of UAV and camera and across the environmental conditions typical for that region. For each crop-genotype combination, the ground truth (for CC) was rapidly estimated using an automated phenomic platform (LeasyScan phenomics platform, ICRISAT). This data-set enabled us to 1) quantify the sensitivity of image acquisition to the main technical, environmental and crop-related factors and this analysis was then used to develop the image acquisition protocols specific to our UAV-camera system. This process was significantly eased by automated ground-truth collection. We also 2) identified the important image quality indicators that integrated the effects of 1) and these indicators were used to develop the quality control protocols for inspecting the images post accquisition. To ease 2), we present a web-based application available at (https://github.com/GattuPriyanka/Framework-for-UAV-image-quality.git) which automatically calculates these key image quality indicators.Overall, we present a methodology for establishing the image acquisition protocol and quality check for obtained images, enabling a high accuracy of plant trait inference. This methodology was demonstrated on a particular UAV-camera set-up and focused on a specific crop trait (CC) at the ICRISAT research station (Hyderabad, India). We envision that, in the future, a similar image quality control system could facilitate the interoperability of data from various UAV-imaging set-ups.

Bupropion (Bup) belongs to the norepinephrine–dopamine reuptake inhibitor (NDRI) class and it is the only FDA-approved drug of its class for the treatment of major depressive disorder (MDD), sold under the name of Wellbutrin. Although bupropion is effective in suppressing the symptoms, its regular use and overdose might lead to seizures and liver failure. Thus, we aimed to nanoformulate bupropion onto a niosomal vesicle to improve its efficacy and achieve the same therapeutic effect at lower scheduled doses. A thin film hydration method was adopted to synthesize and optimize Bup entrapped niosomes using three different surfactants of the sorbitan ester series (Span 20, 40, and 60) in combination with cholesterol. The optimization data determined that the niosome formulated with a cholesterol-to-surfactant ratio of 1:1.5 is the most stable system, with the Bup entrapped niosomes containing Span 20 (Bup@N20C) exhibiting minimal in vitro and in vivo toxicity, and demonstrating the sustained release of Bup in artificial cerebrospinal fluid (ACSF). The Bup@N20C formulation showed increased exploration activity and reduced irregular movements in reserpine-induced depression in the adult zebrafish model, suggesting the potential for mood improvement through the suppression of depression-like behavior which was established by statistical analysis and trajectory data. The Bup@N20C-treated group even surpasses the treatment effect of the positive control group and is comparable to the control group. Hence, it can be inferred that niosomal formulations of Bup represent a promising delivery system capable of achieving the brain delivery of the cargo by bypassing the blood–brain barrier facilitated by their small architectural structure.

This paper outlines experimental and analytical studies focused on strengthened concrete specimens using Engineered Cementitious Composites and use of Kevlar sheets is highlighted as one of the most effective techniques for achieving the desired structural reinforcement and extending the lifespan of structures. The research examines the mechanical properties of retrofitted concrete and material characterizations of ECC such as Scanning Electron Microscopy and Energy dispersive x-ray analyses were also carried out to corroborate the durability properties of ECC and Kevlar-wrapped specimens, specifically assessing compressive, tensile, and flexural strength. In this study, fiber-reinforced cementitious materials featuring a 2% volume fraction of hybrid fibers comprising hooked-end steel and polyvinyl alcohol fibers were employed to strengthen the concrete structure. This additional layer enhances tensile strength and aids in crack management, necessitating proper curing to ensure strength gain over a specified duration. Kevlar fabric sheets are carefully applied to the ECC surface using resin to create a strong bond between the Kevlar and the underlying material, resulting in a durable retrofitted structure. Preliminary experimental data supported numerical modelling of the specimens using finite element analysis. The numerical results regarding the retrofitted strength of hardened concrete were compared with experimental outcomes. The findings showed that the maximum load of the strengthened samples increased by 6.5%. Additionally, the retrofitted strength prior to complete failure rose by 10.6%. In conclusion, the integration of hybrid fibers for reinforcement and Kevlar for retrofitting proves to be a cost-effective and straightforward approach.

In this article, five high gain topologies are derived by reconfiguring the conventional quadratic boost converter. This restructuring is carried out by integrating high gain cells and modifying the topology. The voltage conversion ratio of the topologies is derived using steady-state analysis. Each topology is compared with alike converters in the literature with respect to voltage gain, stresses across the devices and total component count. With this comparative study, the superiority of the suggested topologies is highlighted. Finally, the simulation results of the five topologies are obtained from PSIM software and the results are presented to validate the theoretical results.

Bacterial infections can lead to the formation of chronic wounds and delay the wound-healing process. Therefore, it is important to explore safe and efficient antimicrobial agents that have wound-healing and biocompatible properties. In this study, novel starch-fabricated silver nitroprusside nanoparticles (S-AgNP NPs) were prepared for biocompatible wound-healing applications. The study showed that S-AgNP NPs are spherical, with an average size of 356 ± 22.28 d. nm and zeta potential of −27.8 ± 2.80 mV, respectively. Furthermore, the FTIR and XRD results showed that S-AgNP NPs have functional groups and crystal structures from the silver nitroprusside nanoparticles (AgNP NPs) and starch. Additionally, S-AgNP NPs showed excellent bacterial and biofilm inhibition on B. cereus (15.6 μg/mL), L. monocytogenes (15.6 μg/mL), S. aureus (31.3 μg/mL), E. coli (31.3 μg/mL) and S. enterica (62.5 μg/mL). Moreover, S-AgNP NPs promoted cell migration and proliferation at a concentration of 62.5 μg/mL compared to AgNP NPs. Meanwhile, S-AgNP NPs had good biocompatibility and low cytotoxicity compared to AgNP NPs. Therefore, this study provided new ideas for the development of wound-healing agents with bacteriostatic properties in chronic wounds.

Banana (Musa acuminate) has been popular among consumers worldwide due to its rich nutrients and minerals. However, bananas are highly susceptible to the physical and biological factors that lead to postharvest loss during transportation and storage. In this work, novel sodium alginate (SA) films incorporated with silver nitroprusside nanoparticles (AgNNPs) were prepared to extend the shelf life of bananas through antibacterial and antioxidant coating. The results exhibited that AgNNPs were cubical and that their size was L. monocytogenes, S. enterica, and E. coli at the concentration of 500 µg/mL. Moreover, during the storage of bananas, SA-AgNNPs nanocomposite coatings act as a barrier to microbial contamination and slow down the ripening of bananas. As a result, compared with SA-coated and uncoated bananas, SA-AgNNPs-coated bananas exhibited the lowest weight loss and lowest total bacterial colonies, thus greatly extending their shelf life. Particularly when coated with SA-AgNNPs films, total bacterial colonies (TBC) in the banana peel and pulp were as low as 1.13 × 103 and 51 CUF/g on the ninth day of storage, respectively. Our work offers an efficient strategy to improve the quality of bananas during the postharvest period, with extensive applications in fruit preservation and food packing.

Nicotina stomatitis is the inflammation of the minor salivary glands present in the hard palate due to chronic smoking habit. The patients are mostly symptomless and present with multiple red discrete papules in the hard palate surrounded by grayish white areas. The histopathology of the lesion is discussed and cessation of the smoking habit treats the condition within 2-4 weeks.

In recent times, homotypic and heterotypic 3D tumor spheroid (HTS) models have been receiving increasing attention and come to be widely employed in preclinical studies. The present study is focused on the generation of homotypic (A549 and MDA-MB-231, separately) and heterotypic (A549 + NIH/3T3; MDA-MB-231 + NIH/3T3) 3D tumor spheroids by using GrowDex® nanofibrillar cellulosic (NFC) hydrogel as the scaffold. Light microscopic observations and F-actin staining confirmed the formation of spheroids. The proliferation efficiency indicated an expansion of cell population and an increase in spheroid size over time. The distribution, interaction pattern and influence of fibroblasts on the epithelial cell types were observed in terms of the size and shape of the HTS against homo-spheroids. An interesting observation was that, with an increase in the size of HTSs, many more fibroblast cells were found to occupy the core region, which, perhaps, was due to the faster growth of tumor cells over normal cells. Thus, normal and tumor cells, especially with origins from two different species, can be cultured together in 3D format, and this can potentially enhance our knowledge of tumor microenvironments and cell-cell interaction. These spheroids could be used to improve microphysiological systems for drug discovery and to better understand the tumor microenvironment.

Background and Purpose: Modern technologies are making advanced paths to address emerging issues. The development of carbon dots (CDs) technology at a tiny level has been researched to have made impeccable strides in advancing the modern scientific field, especially in nanomedicine. Experimental Approach: Researchers have gained much attention on CDs of their unique properties in the synthesis, easy surface modifications, excellent optical properties, low toxicity, and water solubility. Doping carbon dots with other elements makes them more convenient for their use in the medical sector. Key Results: The manuscript provides a detailed discussion of the two main methods, including the hydrothermal pathway. CDs are synthesized bottom-up by building up molecules at the atomic scale and top-down by transforming large carbon particles into nanoscale dimensions. Conclusion: The present article discussed the role, importance, and recent advancements in the synthesis of CDs, by using various approaches giving importance to the hydrothermal process. Recent investigations, their mechanism, and theranostic applications have also been reported.