201. Serum MMP7, MMP10 and MMP12 level as negative prognostic markers in colon cancer patients
- Author
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Lena Neumann, Alexis Ulrich, Christoph Kahlert, Fee Klupp, Moritz Koch, Martin Schneider, Johannes Diers, Thomas Schmidt, Juergen Weitz, Niels Halama, and Clemens Franz
- Subjects
Serum ,Male ,0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Survival ,Angiogenesis ,Colorectal cancer ,MMP10 ,Matrix metalloproteinase ,MMP7 ,03 medical and health sciences ,Prognostic marker ,610 Medical sciences Medicine ,0302 clinical medicine ,Matrix Metalloproteinase 10 ,Surgical oncology ,Matrix Metalloproteinase 12 ,Internal medicine ,Biomarkers, Tumor ,Genetics ,medicine ,Humans ,MMP ,business.industry ,Middle Aged ,Prognosis ,medicine.disease ,Colon cancer ,030104 developmental biology ,Matrix Metalloproteinase 7 ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,Cohort ,Adenocarcinoma ,Female ,business ,Research Article - Abstract
Background Matrixmetalloproteinases (MMPs) comprise a family of zinc-dependent endopeptidases which are involved in angiogenesis, tumor invasion and metastatic formation. Up to date, the prognostic relevance of MMPs in serum of patients with colon cancer remains unknown. Thus, we wanted to assess an expression pattern of MMPs in a homogenous cohort of colon cancer patients to assess their potential as prognostic biomarkers. Methods Differences in the expression pattern of MMP7, MMP10 and MMP12 in 78 serum specimens of patients with an adenocarcinoma of the colon and serum specimens of a healthy control group were assessed using Luminex-100 technologies. Subsequently, we correlated these results with histopathological and clinical data of the patients. Results Luminex based expression analysis revealed a significant overexpression of MMP7 and an overexpression of MMP10 and MMP12 in the sera of colon cancer patients compared to the healthy control group. Patients with vascular invasion showed a significantly higher MMP12 expression than V0-staged patients. Moreover overexpression of MMP7, MMP10 and MMP12 in colon cancer patients´ sera displayed a significantly impaired overall survival. Multivariate analysis revealed high MMP10 serum levels to be an independent adverse prognostic marker in colon cancer patients. Conclusions Expression patterns of MMP7, MMP10 and MMP12 in colon cancer patients´ sera are different compared to serum specimens of healthy individuals. Furthermore, overexpression of MMP7, MMP10 and MMP12 in colon cancer patients´ sera correlates with a dismal prognosis and may help to stratify patients into different risk groups. Electronic supplementary material The online version of this article (doi:10.1186/s12885-016-2515-7) contains supplementary material, which is available to authorized users.
- Published
- 2016