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2,231 results on '"A. Baudoux"'

210. N<sub>2</sub> fixation in the Mediterranean Sea related to the composition of the diazotrophic community and impact of dust under present and future environmental conditions

Author

Ridame, Céline, primary, Dinasquet, Julie, additional, Hallstrøm, Søren, additional, Bigeard, Estelle, additional, Riemann, Lasse, additional, Van Wambeke, France, additional, Bressac, Matthieu, additional, Pulido-Villena, Elvira, additional, Taillandier, Vincent, additional, Gazeau, Fréderic, additional, Tovar-Sanchez, Antonio, additional, Baudoux, Anne-Claire, additional, and Guieu, Cécile, additional

Published
2022
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211. Catch and release strategy of matrix metalloprotease aptamers via thiol–disulfide exchange reaction on a graphene based electrochemical sensor

Author

Mishyn, Vladyslav, primary, Aslan, Merve, additional, Hugo, Adrien, additional, Rodrigues, Teresa, additional, Happy, Henri, additional, Sanyal, Rana, additional, Knoll, Wolfgang, additional, Baudoux, Florence, additional, Bouchiat, Vincent, additional, Bilyy, Rostyslav O., additional, Boukherroub, Rabah, additional, Sanyal, Amitav, additional, and Szunerits, Sabine, additional

Published
2022
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214. Seasonal temporal dynamics of marine protists communities in tidally mixed coastal waters

Author

Florence Le Gall, Fabienne Rigaut-Jalabert, Samuel Forsans, Colomban de Vargas, Frédéric Mahé, Yann Bozec, Jean-Philippe Gac, Maxime Manno, Sarah Romac, Laure Arsenieff, Fabrice Not, Mark Hoebeke, Thierry Cariou, Eric Goberville, Mariarita Caracciolo, Samuel Chaffron, Loic Guilloux, Anne-Claire Baudoux, Eric Thiébaut, Nathalie Simon, Nicolas Henry, Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff (SBR), and Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0303 health sciences, Biomass (ecology), Biogeochemical cycle, Ecology, 010604 marine biology & hydrobiology, fungi, Protist, Ecological succession, 15. Life on land, Plankton, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, Geography, 13. Climate action, Benthic zone, medicine, Temperate climate, 14. Life underwater, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Relative species abundance, 030304 developmental biology
Abstract

Major seasonal community reorganizations and associated biomass variations are landmarks of plankton ecology. However, the processes determining marine species and community turnover rates have not been fully elucidated so far. Here, we analyse patterns of planktonic protist community succession in temperate latitudes, based on quantitative taxonomic data from both microscopy counts and ribosomal DNA metabarcoding from plankton samples collected biweekly over 8 years (2009-2016) at the SOMLIT-Astan station (Roscoff, Western English Channel). Considering the temporal structure of community dynamics (creating temporal correlation), we elucidated the recurrent seasonal pattern of the dominant species and OTUs (rDNA-derived taxa) that drive annual plankton successions. The use of morphological and molecular analyses in combination allowed us to assess absolute species abundance while improving taxonomic resolution, and revealed a greater diversity. Overall, our results underpinned a protist community characterised by a seasonal structure, which is supported by the dominant OTUs. We detected that some were partly benthic as a result of the intense tidal mixing typical of the French coasts in the English Channel. While the occurrence of these microorganisms is driven by the physical and biogeochemical conditions of the environment, internal community processes, such as the complex network of biotic interactions, also play a key role in shaping protist communities.

Published
2021

217. Delayed Graft Function in Kidney Transplants: Time Evolution, Role of Acute Rejection, Risk Factors, and Impact on Patient and Graft Outcome

Author

Martin Chaumont, Judith Racapé, Nilufer Broeders, Fadoua El Mountahi, Annick Massart, Thomas Baudoux, Jean-Michel Hougardy, Dimitri Mikhalsky, Anwar Hamade, Alain Le Moine, Daniel Abramowicz, and Pierre Vereerstraeten

Subjects
Surgery, RD1-811
Abstract

Background. Although numerous risk factors for delayed graft function (DGF) have been identified, the role of ischemia-reperfusion injury and acute rejection episodes (ARE) occurring during the DGF period is ill-defined and DGF impact on patient and graft outcome remains controversial. Methods. From 1983 to 2014, 1784 kidney-only transplantations from deceased donors were studied. Classical risk factors for DGF along with two novel ones, recipient’s perioperative saline loading and residual diuresis, were analyzed by logistic regression and receiver operating characteristic (ROC) curves. Results. Along with other risk factors, absence of perioperative saline loading increases acute rejection incidence (OR = 1.9 [1.2–2.9]). Moreover, we observed two novel risk factors for DGF: patient’s residual diuresis ≤500 mL/d (OR = 2.3 [1.6–3.5]) and absence of perioperative saline loading (OR = 3.3 [2.0–5.4]). Area under the curve of the ROC curve (0.77 [0.74–0.81]) shows an excellent discriminant power of our model, irrespective of rejection. DGF does not influence patient survival (P=0.54). However, graft survival is decreased only when rejection was associated with DGF (P

Published
2015
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218. Multi-nomenclature, multi-resolution joint translation: an application to land-cover mapping.

Author

Baudoux, Luc, Inglada, Jordi, and Mallet, Clément

Subjects
*SURFACE of the earth, *CONVOLUTIONAL neural networks, *SPATIAL resolution, *NATURAL languages
Abstract

Land-use/land-cover (LULC) maps describe the Earth's surface with discrete classes at a specific spatial resolution. The chosen classes and resolution highly depend on peculiar uses, making it mandatory to develop methods to adapt these characteristics for a large range of applications. Recently, a convolutional neural network (CNN)-based method was introduced to take into account both spatial and geographical context to translate a LULC map into another one. However, this model only works for two maps: one source and one target. Inspired by natural language translation using multiple-language models, this article explores how to translate one LULC map into several targets with distinct nomenclatures and spatial resolutions. We first propose a new data set based on six open access LULC maps to train our CNN-based encoder-decoder framework. We then apply such a framework to convert each of these six maps into each of the others using our Multi-Landcover Translation network (MLCT-Net). Extensive experiments are conducted at a country scale (namely France). The results reveal that our MLCT-Net outperforms its semantic counterparts and gives on par results with mono-LULC models when evaluated on areas similar to those used for training. Furthermore, it outperforms the mono-LULC models when applied to totally new landscapes. [ABSTRACT FROM AUTHOR]

Published
2023
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222. Les cathédrales d’Arras du Moyen Âge à nos jours

Author

Hanquiez, Delphine, Baudoux-Rousseau, Laurence, Centre de Recherche et d'Etudes - Histoire et Sociétés (CREHS), Université d'Artois (UA), Laurence Baudoux-Rousseau, and Delphine Hanquiez

Subjects
[SHS.ART]Humanities and Social Sciences/Art and art history, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

223. Cotransplantation of Mesenchymal Stem Cells Might Prevent Death from Graft-versus-Host Disease (GVHD) without Abrogating Graft-versus-Tumor Effects after HLA-Mismatched Allogeneic Transplantation following Nonmyeloablative Conditioning

Author

Baron, Frédéric, Lechanteur, Chantal, Willems, Evelyne, Bruck, France, Baudoux, Etienne, Seidel, Laurence, Vanbellinghen, Jean-François, Hafraoui, Kaoutar, Lejeune, Marie, Gothot, André, Fillet, Georges, and Beguin, Yves

Published
2010
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227. Study of the Prevalence of Bovine Brucellosis in the Forest Zone of Cote d’Ivoire

Author

E. Thys, M. A. Yahaya, K. Walravens, C. Baudoux, I. Bagayoko, D. Berkvens, and S. Geerts

Subjects
Bovin, Brucella, Brucellose, Morbidité, Technique immunologique, Zone périurbaine, Forêt, Côte d’Ivoire, Animal culture, SF1-1100
Abstract

A serological survey was carried out in the suburban forest zone of Abidjan from July to September 2004 to estimate the prevalence of brucellosis in dairy cattle. Three hundred and eighty one (381) serums were collected from a total of 927 animals, of which 506 originated from the dairy farms supervised by the South Dairy Project (demonstration farm and private farms), and 421 from neighboring traditional herds. Four serological tests were performed: the rose bengal test, indirect ELISA, the complement fixation test, and the slow agglutination test of Wright with EDTA (micromethod). On the basis of serological and bayesian analyses, seroprevalence rates of about 3.6 and 4.3% were found in the dairy farms and traditional herds, respectively. No significant difference was found between the farms and traditional herds (p < 0.05).

Published
2005
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228. Development of Agropastoralism in a Nomadic Community Settled along the Niger River in Mali

Author

L. Baudoux, H. Kamil, and C.H. Moulin

Subjects
Nomadisme, Système agropastoral, Système de culture, Elevage, Fleuve Niger, Mali, Animal culture, SF1-1100
Abstract

In the region of Timbuktu in Mali, the droughts of the 1970- 80’s and the Tuareg rebellion of the 1990’s led nomadic populations to settlement and cultivation. At the beginning of a new project supporting agropastoral organizations, the NGO Vétérinaires Sans Frontières launched several studies to assess these changes. Thus, we studied the evolution of the agricultural system of an Arab community settled along the Niger River. Three farming systems were characterized. The transhumant agropastoralists (20% of the families) are settled on the Niger banks. They grow rice and burgu grass in the flood plains. They rear small ruminant herds that do not however satisfy all family needs. The nomadic pastoralists (74%) still specialize in livestock farming; livestock management is based on herd and family mobility in order to exploit Sahelian rangelands. At last, semi-nomadic agropastoralists (6%) combine a settled way of life during the dry season on the river banks and nomadism during the rainy season, while salaried employees work in their fields. The study of the relationship between livestock farming and cropping, and of resources use can help shape development policies based on crop intensification on the river banks.

Published
2005
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229. Author response for 'Influenza vaccination and prognosis of COVID ‐19 in hospitalized patients with diabetes: Results from the CORONADO study'

Author

null A Diallo, null M Pichelin, null M Wargny, null P Gourdy, null JB Bonnet, null S Hadjadj, null B Cariou, null A Sultan, null F Galtier, null Matthieu Wargny, null Pascale Mahot, null Bertrand Cariou, null Samy Hadjadj, null Matthieu Pichelin, null Anne‐Laure Fournier‐Guilloux, null Nicolas Mauduit, null Edith Bigot‐Corbel, null Anne‐Sophie Boureau, null Laure Dekcer, null Audrey Ernould, null Claire Primot, null Anne Seguin, null Marielle Joliveau, null Sonia Pouvreau, null Chloé FOURNIER, null Jeremy Thureau, null Edith Fonteneau, null Pamela Hublain, null Chu Nantes, null Carole Agasse, null Mathilde DE Kergaradec, null Vincent Minville, null Fanny Vardon‐Bounes, null Guillaume Martin‐Blondel, null Pierre Gourdy, null Blandine Tramunt, null Marie‐Christine Turnin, null Hélène Hanaire, null Jean‐Michel Mansuy, null Didier Fabre, null Marie‐Blanche Arhainx, null Laurent Cazals, null Laure Combes, null Emmanuelle Lami, null Mallory Cianferani, null Bruno Megarbane, null Pierre Leroy, null Jean‐François Gautier, null Tiphaine Vidal‐Trecan, null Jean‐Pierre Riveline, null Jean‐Louis Laplanche, null Stéphane Mouly, null Louis Potier, null Ronan Roussel, null Malak Taher, null Yawa Abouleka, null Fetta Yaker, null Aurelie Carlier, null Anne Boutten, null Marilyne Hallot‐Feron, null Fadila Mourah, null Charles Thivolet, null Emilie Blond, null Muriel Rolland, null Josep Verdecho Mendez, null Marine Alexandre, null Julien Pottecher, null Emilie Richer, null Laurent Meyer, null Florina Luca, null Jean‐Marc Lessinger, null Thibault Bahougne, null Bruno Guerci, null Lisa Ludwig, null Siham Benzirar, null Catherine Malaplate, null Thierry Matton, null Julien Poissy, null Karine Faure, null Pierre Fontaine, null Florence Baudoux, null Anne Vambergue, null Jean David Pekar, null Marc Lambert, null Cécile Yelnik, null Amélie Bruandet, null Laurent Petit, null Didier Neau, null Vincent Rigalleau, null Annie Berard, null Amandine Galioot, null Remy Coudroy, null Arnaud Thille, null René Robert, null France Roblot‐Cazenave, null Blandine Rammaert, null Pierre Jean Saulnier, null Xavier Piguel, null Nesrine Benhenda, null Camille Husson, null Celine Olivier, null Florence Torremocha, null Mathilde Fraty, null Marie Flamen d'assigny, null Aurelie Miot, null Valentin Bossard, null Kada Klouche, null Alain Makinson, null Ariane Sultan, null Jean‐Baptiste Bonnet, null Vincent Foulongne, null Florence Galtier, null Cécile Aubron, null Séverine Ansart, null Véronique Kerlan, null Pascale Quiniou, null Jean‐Luc Carre, null Stéphane Quesnot, null Bruno Laviolle, null Carole Schwebel, null Olivier Epaulard, null Pierre‐Yves Benhamou, null Cécile Betry, null Anne‐Laure Borel, null Sandrine Lablanche, null Dorra Guergour, null Catherine Duclos, null Emmanuel Cosson, null Erwan Guyot, null Aurore Deniau, null Phucthutrang Nguyen, null Yves Reznik, null Michael Joubert, null Stéphane Allouche, null Lydia Guittet, null Steven Grange, null Manuel Etienne, null Gaëtan Prévost, null Valéry Brunel, null Jean‐Christophe Lagier, null Didier Raoult, null Anne Dutour, null Bénédicte Gaborit, null Sandrine Boulllu, null Patrice Darmon, null Adèle Lasbleiz, null Mathieu Cerino, null Fanny Romain, null Marie Houssays, null Jean Pierre Quenot, null Lionel Piroth, null Bruno Vergès, null Laurence Duvillard, null Bernard Bonnotte, null Alain Mercat, null Vincent Dubee, null Ingrid Allix, null Patrice Rodien, null Robin Dhersin, null Maylis Lebeault, null Wojciech Trzepizur, null Jocelyne Loison, null Antoine Brangier, null Pierre Asfar, null Pascal Reynier, null Françoise Larcher, null Françoise Joubaud, null Marie‐Rita Andreu, null Geoffrey Urbanski, null Laurent Hubert, null Cedric Annweiler, null Jean Dellamonica, null Johan Courjon, null Nicolas Chevalier, null Giulia Chinetti, null Magda Chafai, null Bruno Mourvillier, null Firouze Bani‐Sadr, null Sarra Barraud, null Brigitte Delemer, null Philippe Gillery, null Pascale Labedade, null Amélie Chabrol, null Alfred Penfornis, null Catherine Petit, null Coralie Amadou, null Maxime Adler, null Clément Dubost, null Pierre‐Louis Conan, null Lyse Bordier, null Franck Ceppa, null Cyril Garcia, null Mathilde Sollier, null Olivier Dupuy, null Sophie Laplance, null Olivier Billuart, null Marie Joseph Aroulanda, null Frédérique Olivier, null Florence Ayon, null Nathalie Wilhelm, null Loic Epelboin, null Nadia Sabbah, null Aurelie Charpin, null Pierre Squara, null Olivier Belliard, null Claude Dubois, null Michel Marre, null Johann Auchabie, null Roxane Courtois, null Thierry Duriez, null Tiphaine Mergey, null Laura Vallee, null Laetitia Seguin, null Abdallah Al‐Salameh, null Jean‐Philippe Lanoix, null Sandrine Soriot‐Thomas, null Anne‐Marie Bourgeois‐Descouls, null Rachel Desailloud, null Natacha Germain, null Bogdan Galusca, null Gwenaelle Belleton, null Nesrine Marouani, null Delia Palaghiu, null Amira Hammour, null Fernando Berdaguer, null Thimothée Klopfenstein, null Hajer Zayet, null Patrice Winiszewski, null Marie Zanusso, null Pauline Garnier, null Ingrid Julier, null Karim Hamzaoui, null Sophie Marty‐Gres, null Tarik Sadki, null Lucile Cadot, null Jean‐Louis Dubost, null Céline Gonfroy, null Catherine Campinos, null Pascale Martres, null Marie Pierre Coulhon, null Nicolas Allou, null Marwa Bachir, null Stella Hoang, null Candice Kembellec, null Olivia Suply, null Fatima Kharcha, null Anne‐Claire Devouge, null Anna Flaus‐Furmanuk, null Isabelle Madeline, null Vincent Ehinger, null Sophie Bastard, null Loic Raffray, null Frederic Renou, null Aude Bojarski, null Caroline Paul, null Karine Borsu, null Angelique Gorlin, null Servane Bernardo, null Carole Truong Ut, null Stephane Renaud, null Antoine Vignoles, null Emilie Foch, null Laurie Masse, null Hubert Grand, null Helene Ferrand, null Christelle Raffaitin‐Cardin, null Hadjer Zellagui, null Celine Castang‐Brachet, null Frederique Boury, null Ana Alvarez Tena, null Isabelle Moura, null Pierre Kalfon, null Juliana Darasteanu, null Arnaud Monier, null Pascal Foucault, null Alexandra Depuille, null Stéphanie Laugier‐Robiolle, null Patrick Caneiro, null Maud Basso, null Etienne Larger, null Samir Bouam, null Wahiba Benzenati, null Leila Ait Bachir, null Camille Cussac Pillegand, null Marc Vasse, null Christophe Michard, null Nathanaëlle Montanier, null Luc Millot, null Françoise Crepet, null Danielle Ratsimba, null Kevin Bouiller, null Sophie Borot, null Isabelle Bruckert, null Annie Clergeot, null Franck Schillo, null Dorothée Vignes, null Muriel Bourgeon‐Ghittori, null Hamoud Lachgar, null Claire Lambert DE Cursay, null Stéphane Levante, null Jean Charles Auregan, null Antoine Merlet, null Cécile Zaragoza, null Gwénaëlle Arnault, null Anne‐Gaëlle Loupp, null Olivier Lesieur, null Mariam Roncato‐Saberan, null Didier Gouet, null Romain Lemarie, null Hong_an Allano, null Emmanuel Vivier, null Caroline Pariset, null Cédric Luyton, null Lucien Marchand, null Fanny Doroszewski, null Matthieu Pecquet, null Laurent Perard, null Sylvie Vuillermoz‐Blas, null Nicolas Kacki, null Patricia Charrier, null Amélie Ducet‐Boiffard, null Françoise Desroys Roure, null Olivier Bourron, null Dominique Bonnefont‐Rousselot, null Suzanne Laroche, null Franck Phan, null Agnès Hartemann, null Cyrielle Caussy, null Emmanuel Disse, null Claude Guerin, null Thomas Perpoint, null Philippe Moulin, null Régine Cartier, null Geoffroy Hariri, null Dorothée Chopin, null Camille Vatier, null Nathalie Bourcigaux, null Emmanuelle Chaigneau, null Sophie Christin‐Maitre, null Bruno Donadille, null Bruno Feve, null Sophie Lamothe, null Julie Sarfati, null Pascal Pernet, null Anne Chambon, null Delphine Demarsy, null Hugo Campagne, null Françoise Latil‐Plat, null Monica Berne, null Marilyne Grinand, null Marion Touzet, null Aydrey Zabulon, null Jocelyne Craspag, null Catherine Ledoux, null Cedric Contaret, null Blandine Janand‐Delenne, null Anaïs Giraud, null Marie Lou Lacrimini, null Joëlle Arrivie, null Deborah Ancelle, null Carine Guillois, null Bénédicte Fremy, null Amina Chaalal, null Gaëlle Barrande, null Anne Dorange, null Eglantine Rouanet, null Dominique Seret‐Begue, null Audrey Saoud, null Anne‐Marie Guedj, null Nathalie Bedos, null Fritz‐Line Velayoudom, null Marie Dumas, null Benoite Gonda, null Christine Coffin, null Stéphanie Gibiat, null Myriam Lungo, null Chantal Bully, null Pierre Serusclat, null Stella Bully, null Patricia Carre, null Jean‐Philippe Leberre, null Carlos Elkhoury, null Marine Thieux, null Laetitia Paradisi‐Prieur, and null CORONADO investigators

Subjects
Vaccination, Pediatrics, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), business.industry, Hospitalized patients, Diabetes mellitus, Medicine, business, medicine.disease
Published
2021
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230. Mesenchymal Stem Cell Injection in Crohn's Disease Strictures: A Phase I-II Clinical Study

Author

Chantal Lechanteur, Catherine Reenaers, Layla Boutaffala, Alexandra Briquet, Jean-Philippe Loly, Paul Meunier, Etienne Baudoux, Edouard Louis, Sophie Vieujean, and Yves Beguin

Subjects
Crohn's disease, medicine.medical_specialty, medicine.diagnostic_test, business.industry, Mesenchymal stem cell, Gastroenterology, Mesenchymal Stem Cells, General Medicine, Constriction, Pathologic, Anastomosis, medicine.disease, Endoscopy, Gastrointestinal, Surgery, Endoscopy, Clinical study, Phase i ii, Treatment Outcome, Crohn Disease, Occlusion, Medicine, Humans, business, Adverse effect
Abstract

Background and Aim Mesenchymal stem cells [MSCs] have anti-inflammatory and anti-fibrotic properties and could be a potential therapy for Crohn’s disease [CD] strictures. In this phase I–II pilot trial, we assessed safety and efficacy of local MSC injection to treat CD strictures. Methods CD patients with a short [less than 5 cm in length] non-passable stricture accessible by ileocolonoscopy were included. Allogenic bone-marrow derived MSCs were injected in the four quadrants of the stricture. Adverse events and clinical scores were evaluated at each follow-up visit and endoscopy and magnetic resonance enterography were performed at baseline, Week [W]12 and W48. The main judgement criterion for efficacy was the complete [defined by the ability to pass the ileocolonoscope] or partial [defined by a diameter increase] resolution of the stricture at W12. Second efficacy criteria included assessment of the stricture at W48 and evolution of clinical scores at W12 and W48. Results We performed 11 MSC injections in 10 CD patients [three primary and seven anastomotic strictures; one stricture injected twice]. MSC injections were well tolerated but four hospitalisations for occlusion were reported. At W12, five patients presented a complete or partial resolution of the stricture [two complete and three partial]. Seven patients were re-evaluated at W48 [one dilated, one operated, and one lost to follow-up] and four patients had a complete resolution. The evolution of clinical scores between W0, W12, and W48 was not statistically significant. Conclusions MSCs injection in CD stricture was well tolerated and may offer a benefit.

Published
2021

231. N2 fixation in the Mediterranean Sea related to the composition of the diazotrophic community, and impact of dust under present and future environmental conditions

Author

Céline Ridame, Julie Dinasquet, Søren Hallstrøm, Estelle Bigeard, Lasse Riemann, France Van Wambeke, Matthieu Bressac, Elvira Pulido-Villena, Vincent Taillandier, Frederic Gazeau, Antonio Tover-Sanchez, Anne-Claire Baudoux, and Cécile Guieu

Subjects
0106 biological sciences, 010504 meteorology & atmospheric sciences, 010604 marine biology & hydrobiology, Mineral dust, 01 natural sciences, Grazing pressure, Mediterranean sea, Nutrient, Agronomy, 13. Climate action, Nitrogen fixation, Environmental science, Photic zone, 14. Life underwater, Relative species abundance, Ecology, Evolution, Behavior and Systematics, Nutrient bioavailability, 0105 earth and related environmental sciences, Earth-Surface Processes
Abstract

N2 fixation rates were measured in the 0–1000 m layer at 13 stations located in the open western and central Mediterranean Sea (MS) during the PEACETIME cruise (late spring 2017). While the spatial variability in N2 fixation was not related to Fe, P nor N stocks, the surface composition of the diazotrophic community indicated a strong longitudinal gradient increasing eastward for the relative abundance of non-cyanobacterial diazotrophs (NCDs) (mainly γ-Proteobacteria) and conversely decreasing eastward for photo-heterotrophic group A (UCYN-A) (mainly UCYN-A1 and UCYN-A3), as did N2 fixation rates. UCYN-A4 and UCYN-A3 were identified for the first time in the MS. The westernmost station influenced by Atlantic waters and characterized by highest stocks of N and P displayed a patchy distribution of diazotrophic activity with an exceptionally high rate in the euphotic layer of 72.1 nmolNL-1d-1, which could support up to 19 % of primary production. At this station at 1 % PAR (photosynthetically available radiation) depth, UCYN-A4 represented up to 94 % of the diazotrophic community. These in situ observations of greater relative abundance of UCYN-A at stations with higher nutrient concentrations and dominance of NCDs at more oligotrophic stations suggest that nutrient conditions – even in the nanomolar range – may determine the composition of diazotrophic communities and in turn N2 fixation rates. The impact of Saharan dust deposition on N2 fixation and diazotrophic communities was also investigated, under present and future projected conditions of temperature and pH during short-term (3–4 d) experiments at three stations. New nutrients from simulated dust deposition triggered a significant stimulation of N2 fixation (from 41 % to 565 %). The strongest increase in N2 fixation was observed at the stations dominated by NCDs and did not lead on this short timescale to changes in the diazotrophic community composition. Under projected future conditions, N2 fixation was either increased or unchanged; in that later case this was probably due to a too-low nutrient bioavailability or an increased grazing pressure. The future warming and acidification likely benefited NCDs (Pseudomonas) and UCYN-A2, while disadvantaged UCYN-A3 without knowing which effect (alone or in combination) is the driver, especially since we do not know the temperature optima of these species not yet cultivated as well as the effect of acidification.

Published
2021
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233. N2 fixation in the Mediterranean Sea related to the composition of the diazotrophic community, and impact of dust under present and future environmental conditions

Author

Ridame, Céline, Dinasquet, Julie, Hallstrøm, Søren, Bigeard, Estelle, Riemann, Lasse, van Wambeke, France, Bressac, Matthieu, Pulido-Villena, Elvira, Taillandier, Vincent, Gazeau, Frédéric, Tovar-Sánchez, Antonio, Baudoux, Anne-Claire, Guieu, Cécile, Cycles biogéochimiques marins : processus et perturbations (CYBIOM), Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN), Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Scripps Institution of Oceanography (SIO - UC San Diego), University of California [San Diego] (UC San Diego), University of California (UC)-University of California (UC), Laboratoire d'Océanographie Microbienne (LOMIC), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), University of Copenhagen = Københavns Universitet (UCPH), Adaptation et diversité en milieu marin (ADMM), Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Institute of Marine Sciences of Andalusia (ICMAN), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Sorbonne Université (SU), Centre National de la Recherche Scientifique (CNRS), Institut Pierre-Simon-Laplace (IPSL (FR_636)), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut de Recherche pour le Développement (IRD)-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU), Scripps Institution of Oceanography (SIO), University of California-University of California, Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), University of Copenhagen = Københavns Universitet (KU), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), PEACETIME project (http://peacetime-project.org), GEOTRACES, Centre National de la Recherche Scientifique (France), European Commission, Danish Council for Independent Research, and Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDU]Sciences of the Universe [physics], [SDE]Environmental Sciences, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
Abstract

N2 fixation rates were measured in the 0-1000ĝ€¯m layer at 13 stations located in the open western and central Mediterranean Sea (MS) during the PEACETIME cruise (late spring 2017). While the spatial variability in N2 fixation was not related to Fe, P nor N stocks, the surface composition of the diazotrophic community indicated a strong longitudinal gradient increasing eastward for the relative abundance of non-cyanobacterial diazotrophs (NCDs) (mainly 3-Proteobacteria) and conversely decreasing eastward for photo-heterotrophic group A (UCYN-A) (mainly UCYN-A1 and UCYN-A3), as did N2 fixation rates. UCYN-A4 and UCYN-A3 were identified for the first time in the MS. The westernmost station influenced by Atlantic waters and characterized by highest stocks of N and P displayed a patchy distribution of diazotrophic activity with an exceptionally high rate in the euphotic layer of 72.1ĝ€¯nmolNL-1d-1, which could support up to 19ĝ€¯% of primary production. At this station at 1ĝ€¯%PAR (photosynthetically available radiation) depth, UCYN-A4 represented up to 94ĝ€¯% of the diazotrophic community. These in situ observations of greater relative abundance of UCYN-A at stations with higher nutrient concentrations and dominance of NCDs at more oligotrophic stations suggest that nutrient conditions-even in the nanomolar range-may determine the composition of diazotrophic communities and in turn N2 fixation rates. The impact of Saharan dust deposition on N2 fixation and diazotrophic communities was also investigated, under present and future projected conditions of temperature and pH during short-Term (3-4ĝ€¯d) experiments at three stations. New nutrients from simulated dust deposition triggered a significant stimulation of N2 fixation (from 41ĝ€¯% to 565ĝ€¯%). The strongest increase in N2 fixation was observed at the stations dominated by NCDs and did not lead on this short timescale to changes in the diazotrophic community composition. Under projected future conditions, N2 fixation was either increased or unchanged; in that later case this was probably due to a too-low nutrient bioavailability or an increased grazing pressure. The future warming and acidification likely benefited NCDs (Pseudomonas) and UCYN-A2, while disadvantaged UCYN-A3 without knowing which effect (alone or in combination) is the driver, especially since we do not know the temperature optima of these species not yet cultivated as well as the effect of acidification., This study is a contribution to the PEACETIME project (http://peacetime-project.org, last access: 17 January 2022), a joint initiative of the MERMEX and ChArMEx components supported by the CNRS-INSU, IFREMER, CEA and Météo-France as part of the program MISTRALS coordinated by the INSU. PEACETIME was endorsed as a process study by GEOTRACES. Julie Dinasquet was funded by a Marie Curie Actions International Outgoing Fellowship (PIOF-GA-2013-629378). Søren Hallstrøm and Lasse Riemann were funded by grant 6108-00013 from the Danish Council for Independent Research.

Published
2021
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234. Contextual Land-Cover Map Translation with Semantic Segmentation

Author

Jordi Inglada, Luc Baudoux, and Clément Mallet

Subjects
Transformation (function), Computer science, business.industry, Encoding (memory), Pattern recognition, Segmentation, Land cover, Artificial intelligence, Semantics, business, Translation (geometry), Convolutional neural network, Image resolution
Abstract

This paper presents a framework for translating a land-cover map into another one in a supervised way. This links to numerous applications (updating, completion, etc.). Conversely to existing approaches, we jointly perform spatial and semantic transformation without any prior knowledge. The proposed method assumes that: i) examples of the source and target maps already exist, ii) the spatial resolution of the source map is equal or higher than the target one. The translation is performed using an asymmetric Convolutional Neural Network with positional encoding. Experimental results show the effectiveness of the method in retrieving a yearly version of Corine Land Cover (CLC) at country-scale (France) using an existing high-resolution map and with similar accuracy than existing CLC maps (~80%).

Published
2021
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235. Fonctionnalisation de surfaces de zircone par photopolymérisation de monomères zwittérioniques

Author

Dezanet, Clément, Lepoittevin, Bénédicte, Baudoux, Jérôme, Rouden, Jacques, Lecourt, Jérôme, Harnois, Christelle, Fouchet, Arnaud, Froissart, Sandrine, Marie, Philippe, Thebault, Pascal, Laboratoire de chimie moléculaire et thioorganique (LCMT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Le Havre Normandie (ULH), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de cristallographie et sciences des matériaux (CRISMAT), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Nanomatériaux, Ions et Métamatériaux pour la Photonique (NIMPH), Centre de recherche sur les Ions, les MAtériaux et la Photonique (CIMAP - UMR 6252), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Polymères Biopolymères Surfaces (PBS), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), and Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

241. Did Osteoblastic Cell Therapy Improve the Prognosis of Pre-fracture Osteonecrosis of the Femoral Head? A Randomized, Controlled Trial

Author

Michel Malaise, Sanjiva Pather, Etienne Baudoux, Jean-Philippe Hauzeur, Viviane De Maertelaer, Chantal Lechanteur, Raphael Katz, Yves Beguin, and Julia Ino

Subjects
medicine.medical_specialty, WOMAC, medicine.medical_treatment, Cell- and Tissue-Based Therapy, law.invention, Fractures, Bone, 03 medical and health sciences, Femoral head, 0302 clinical medicine, Randomized controlled trial, Clinical Research, Femur Head Necrosis, law, Humans, Medicine, Orthopedics and Sports Medicine, 030212 general & internal medicine, Stage (cooking), Adverse effect, 030222 orthopedics, business.industry, Hazard ratio, Osteonecrosis, Femur Head, General Medicine, Hepatitis B, Prognosis, medicine.disease, Arthroplasty, Surgery, medicine.anatomical_structure, business
Abstract

Background In patients with nontraumatic osteonecrosis of the femoral head (ONFH), implantation of bone marrow aspirate concentrate (BMAC) could delay the progression of osteonecrosis and improve symptoms in pre-fracture ONFH. However, the BMAC content, especially in osteoblastic stem cells, could have an important individual variability. An autologous osteoblastic cell product could improve the effect of such cell-based therapy. Questions/purposes (1) Does autologous osteoblastic cell therapy decrease the likelihood of progression to subchondral fracture with or without early collapse corresponding to Association Research Circulation Osseous (ARCO) classification Stage III or higher, and provide a clinically important pain improvement compared with BMAC treatment alone? (2) Were patients treated with osteoblastic cell therapy less likely to undergo subsequent THA? (3) What proportion of patients in the treatment and control groups experienced adverse events after surgery? Methods Between 2004 and 2011, we treated 279 patients for Stage I to II hip osteonecrosis (ON) with surgery. During that time, our general indications for surgery in this setting included non-fracture ON lesions. To be eligible for this randomized, single-blind trial, patients needed to have an ONFH Stage I or II; we excluded those with traumatic ONFH, hemoglobinopathies and positive serology for hepatitis B, C or HIV. Of those treated surgically for this diagnosis during the study period, 24% (67) agreed to participate in this randomized trial. Hips with pre-fracture ONFH were randomly treated with a core decompression procedure associated with either implantation of a BMAC (BMAC group; n = 26) or osteoblastic cell (osteoblastic cell group; n = 30). The groups were not different in terms of clinical and imaging characteristics. The primary study outcome was treatment response, defined as the absence of progression to subchondral fracture stage (ARCO stage III or higher) plus a clinically important pain improvement defined as 1 cm on a 10-cm VAS. The secondary endpoint of interest was the frequency in each group of subsequent THA and the frequency of adverse events. The follow-up duration was 36 months. We used an as-treated analysis (rather than intention-to-treat) for our efficacy endpoint, and an intention-to-treat analysis for adverse events. Overall, 26 of 26 patients in the BMAC group and 27 of 30 in the osteoblastic cell group completed the trial. Results At 36 months, no clinically important differences were found in any study endpoint. There was no difference in the proportion of patients who had progressed to fracture (ARCO stage III or higher; 46% of the BMAC hips [12 of 26] versus 22% in the hips with osteoblastic cells [six of 27], hazard ratio, 0.47 [95% CI 0.17 to 1.31]; p = 0.15). There was no clinically important difference in VAS pain scores. No differences were found for either the WOMAC or the Lequesne indexes. With the numbers available, there was no difference in the proportion of patients in the groups who underwent THA at 36 months 15% (four of 27) with osteoblastic cells versus 35% (nine of 26) with BMAC; p = 0.09 With the numbers available, we found no differences between the treatment and control groups in terms of the frequencies of major adverse events. Conclusions We found no benefit to osteoblastic cells over BMAC in patients with pre-collapse ONFH; side effects were uncommon and generally mild in both groups. This study could be used as pilot data to help determine sample sizes for larger (presumably multicenter) randomized controlled trials. However, this novel treatment cannot be recommended in routine practice until future, larger studies demonstrate efficacy. Level of evidence Level II, therapeutic study.

Published
2019
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242. Luxation radio-carpienne : étude rétrospective de 14 patients

Author

Nadine Sturbois-Nachef, Matthieu Baudoux, Christophe Chantelot, Thomas Amouyel, Marc Saab, Alexandre Cornu, and Hôpital Jeanne de Flandre [Lille]

Subjects
030222 orthopedics, 03 medical and health sciences, 0302 clinical medicine, [SDV]Life Sciences [q-bio], Orthopedics and Sports Medicine, Surgery, 030230 surgery
Abstract

Resume Introduction La luxation radio-carpienne (LRC) se definit par une perte de contact totale des surfaces articulaires du carpe avec la surface articulaire radiale. Rare, peu de series ont ete publiees sur le sujet. L’objectif de notre etude etait d’apprecier les resultats fonctionnels apres chirurgie, en particulier sans reparation ligamentaire, d’une LRC. Hypothese Le resultat fonctionnel postoperatoire a distance est satisfaisant pour une utilisation du poignet dans les activites de vie quotidienne. Materiels et methodes Une etude retrospective a ete menee de janvier 2012 a juillet 2017. Les criteres d’inclusion etaient la presence d’une LRC sur les radiographies preoperatoires chez des sujets majeurs et un recul minimum de 6 mois ; les criteres d’exclusion etaient la presence de cartilages de croissance non fusionnes et une fracture de l’epiphyse radiale distale a grand deplacement. Le type de LRC etait evalue par la classification de Dumontier. Le resultat fonctionnel etait evalue par les amplitudes articulaires postoperatoires du poignet, la force de prehension (Jamar®), les scores fonctionnels QuickDash, et Green O’Brien modifie par Cooney. Resultats Quatorze patients ont ete revus a 31 mois (7–60). Trois patients presentaient une LRC de type I, 11 de type II. Tous les patients ont ete pris en charge chirurgicalement. Aucune suture ligamentaire n’a ete realisee. La flexion moyenne etait de 63° (20–90°), l’extension de 51° (25-90°), la pronation de 79° (60–90), la supination de 80° (50–90), la force de prehension de 27,9 kg (8–40). Le QuickDASH et le score de Green O’Brien modifie par Cooney moyens etaient respectivement de 25,6 (4,54–40,9) et de 74 (35–100). Discussion Nos resultats fonctionnels etaient satisfaisants et comparables a la litterature, et ce, malgre l’absence de suture ligamentaire radio-carpienne, dans les LRC de type I. Niveau de preuve IV.

Published
2019
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243. Self-Catalyzed Coupling between Brønsted-Acidic Imidazolium Salts and Epoxy-Based Materials: A Theoretical/Experimental Study

Author

Rafał Konefał, Libor Matějka, Sébastien Livi, Leandro Seixas, Ricardo K. Donato, Hynek Beneš, Jérôme Baudoux, and Magdalena Perchacz

Subjects
Solvent free, Renewable Energy, Sustainability and the Environment, General Chemical Engineering, Epoxide, 02 engineering and technology, General Chemistry, Epoxy, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Catalysis, chemistry.chemical_compound, chemistry, One pot reaction, visual_art, Ionic liquid, Polymer chemistry, visual_art.visual_art_medium, Environmental Chemistry, 0210 nano-technology
Abstract

Herein we present a comprehensive study on the role of Bronsted-acidic imidazolium ionic liquids (ILs) and imidazolium salts promoting the reaction between carboxyl and epoxide groups in a controll...

Published
2019
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244. Infusion of third-party mesenchymal stromal cells after kidney transplantation: a phase I-II, open-label, clinical study

Author

Hans Pottel, Gianni Maggipinto, Yves Beguin, Pauline Erpicum, Chantal Lechanteur, Céline Gregoire, François Jouret, Etienne Baudoux, Marie-Hélène Delbouille, Joan Somja, Alexandra Briquet, Olivier Detry, Catherine Bonvoisin, Frédéric Baron, and Laurent Weekers

Subjects
Graft Rejection, Male, 0301 basic medicine, medicine.medical_specialty, Regulatory T cell, medicine.medical_treatment, 030232 urology & nephrology, Urology, Renal function, Kidney, Mesenchymal Stem Cell Transplantation, T-Lymphocytes, Regulatory, Cell therapy, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Transplantation, Homologous, Lymphocyte Count, Prospective Studies, Kidney transplantation, Aged, Immunosuppression Therapy, B-Lymphocytes, business.industry, Mesenchymal stem cell, Immunosuppression, Middle Aged, Allografts, medicine.disease, Kidney Transplantation, Transplantation, Treatment Outcome, 030104 developmental biology, medicine.anatomical_structure, Nephrology, Kidney Failure, Chronic, Administration, Intravenous, Female, Bone marrow, business, Follow-Up Studies, Glomerular Filtration Rate
Abstract

Mesenchymal stromal cells (MSCs) exhibit anti-inflammatory and immune-regulatory properties, and preclinical studies suggest a potential benefit in solid organ transplantation. We report on the 1-year follow-up of an open-label phase I-II trial of a single infusion of third-party MSC post-kidney transplantation, in addition to standard immunosuppression. Ten kidney transplant recipients from deceased donors received third-party bone marrow MSCs (∼2 × 106/kg) on day 3 ± 2 post-transplant and were compared to 10 concurrent controls. No adverse effects were noted at MSC injection. One participant with a history of cardiac disease had a non-ST-elevation myocardial infarction approximately 3 hours after MSC infusion. Incidences of opportunistic infections and acute rejection were similar. At day 7 post-transplant, estimated glomerular filtration rate (eGFR) in MSC-treated recipients reached 48.6 ml/min/1.73m2, compared to 32.5 ml/min/1.73m2 in controls and 29.3 ml/min/1.73m2 in our overall cohort of kidney transplant recipients. No difference in eGFR was found at 1 year. MSC-treated recipients showed increased frequencies of regulatory T cells at day 30, with no significant change in B cell frequencies compared to concurrent controls. Four MSC-treated participants developed antibodies against MSC or shared kidney-MSC HLA, with only 1 with MFI >1500. A single infusion of third-party MSC following kidney transplantation appears to be safe, with one cardiac event of unclear relationship to the intervention. MSC therapy is associated with increased regulatory T cell proportion and with improved early allograft function. Long-term effects, including potential immunization against MSC, remain to be studied.

Published
2019
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249. Engraftment kinetics and graft failure after single umbilical cord blood transplantation using a myeloablative conditioning regimen

Author

Annalisa Ruggeri, Myriam Labopin, Maria Pia Sormani, Guillermo Sanz, Jaime Sanz, Fernanda Volt, Gerard Michel, Franco Locatelli, Cristina Diaz De Heredia, Tracey O’Brien, William Arcese, Anna Paola Iori, Sergi Querol, Gesine Kogler, Lucilla Lecchi, Fabienne Pouthier, Federico Garnier, Cristina Navarrete, Etienne Baudoux, Juliana Fernandes, Chantal Kenzey, Mary Eapen, Eliane Gluckman, Vanderson Rocha, and Riccardo Saccardi

Subjects
Diseases of the blood and blood-forming organs, RC633-647.5
Abstract

Umbilical cord blood transplant recipients are exposed to an increased risk of graft failure, a complication leading to a higher rate of transplant-related mortality. The decision and timing to offer a second transplant after graft failure is challenging. With the aim of addressing this issue, we analyzed engraftment kinetics and outcomes of 1268 patients (73% children) with acute leukemia (64% acute lymphoblastic leukemia, 36% acute myeloid leukemia) in remission who underwent single-unit umbilical cord blood transplantation after a myeloablative conditioning regimen. The median follow-up was 31 months. The overall survival rate at 3 years was 47%; the 100-day cumulative incidence of transplant-related mortality was 16%. Longer time to engraftment was associated with increased transplant-related mortality and shorter overall survival. The cumulative incidence of neutrophil engraftment at day 60 was 86%, while the median time to achieve engraftment was 24 days. Probability density analysis showed that the likelihood of engraftment after umbilical cord blood transplantation increased after day 10, peaked on day 21 and slowly decreased to 21% by day 31. Beyond day 31, the probability of engraftment dropped rapidly, and the residual probability of engrafting after day 42 was 5%. Graft failure was reported in 166 patients, and 66 of them received a second graft (allogeneic, n=45). Rescue actions, such as the search for another graft, should be considered starting after day 21. A diagnosis of graft failure can be established in patients who have not achieved neutrophil recovery by day 42. Moreover, subsequent transplants should not be postponed after day 42.

Published
2014
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