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Copyright of China Textile Leader is the property of China Textile Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of China Textile Leader is the property of China Textile Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Textile Leader is the property of China Textile Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The purpose of this experiment is to clarify the regulatory effect of miR-301b on goat intramuscular adipocyte differentiation and to explore the possible mechanism of its regulatory effect. Chemically-synthesized miR-301b mimics and miR-301b siRNA were used to overexpress and interfere miR-301b in goat intramuscular adipocytes by liposome transfection，and the regulatory effect of miR-301b on goat intramuscular adipocyte differentiation was clarified from the cell morphology and mRNA level by oil red O staining，qPCR and bioinformatics analysis，and its possible mechanism was explored preliminary. The results showed that the overexpression efficiency of miR-301b was about 29 290 times. The results of cell morphology showed that the accumulation of lipid droplets and triglyceride synthesis of goat intramuscular adipocytes decreased after the overexpression of miR-301b. qPCR results showed that expressions of lipogenic marker gene CEBPα，PPARγ， SREBP1 and LPL in the overexpressed miR-301b significantly reduced（P<0.01）. The interference efficiency of miR-301b was about 60%. After the expression of miR-301b was interfered，the results of cell morphology showed that the accumulation of lipid droplets in goat intramuscular adipocytes increased and the synthesis of triglycerides increased. qPCR results showed that interfering miR-301b significantly promoted expressions of adipogenic marker gene AP2，CEBPα，CEBPβ，PPARγ，SREBP1 and LPL. Through bioinformatics analysis of miR-301b，it was speculated that KLF3 was the target gene of miR-301b. qPCR verification found that overexpression of miR-301b significantly inhibited the mRNA expression of KLF3，and interference to miR-301b significantly promoted the expression of KLF3. In conclusion， overexpression of miR-301b inhibited the differentiation of intramuscular preadipocytes in goats，and down-regulated the expression of KLF3 ； interfering miR-301b promoted the differentiation of intramuscular preadipocytes in goats，and the expression of KLF3 was up-regulated. Thus it is speculated that miR-301b may inhibit the differentiation of goat intramuscular adipocytes through KLF3. [ABSTRACT FROM AUTHOR]