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385 results on '"von dem Bussche A"'

151. Semi-quantification of human C-C chemokine mRNAs with reverse transcription/real-time PCR using multi-specific standards

Author

Hans Dieter Nischalke, Jürgen K. Rockstroh, Franz Ludwig Dumoulin, Tilman Sauerbruch, Ulrich Spengler, Annette von dem Bussche, and Ludger Leifeld

Subjects
Chemokine, Immunology, Biology, law.invention, law, Transcription (biology), Complementary DNA, Gene expression, Immunology and Allergy, Humans, RNA, Messenger, Chemokine CCL4, Chemokine CCL5, Polymerase chain reaction, Chemokine CCL2, Liver Cirrhosis, Biliary, Reverse Transcriptase Polymerase Chain Reaction, Hepatitis C, Chronic, Macrophage Inflammatory Proteins, Molecular biology, Reverse transcriptase, Housekeeping gene, Real-time polymerase chain reaction, Liver, Chemokines, CC, biology.protein
Abstract

A reverse transcription/real-time polymerase chain reaction (PCR) assay was established to semi-quantify the mRNA levels of the human C-C chemokines RANTES, MIP-1beta and MCP-1 relative to the housekeeping gene beta-actin. The assay showed a high sensitivity (below 60 cDNA molecules/10 microl reaction) and dynamic range (8 log units); both within-assay and inter-assay variability were below 0.06 log units and the accuracy was +/-0.06 log units for all four chemokines. Moreover, it is demonstrated that a multi-specific DNA fragment, which had previously been constructed for competitive PCR, can be used as a reliable external standard. This allows a direct semi-quantitative comparison of different chemokine mRNA levels and is a convenient alternative to the use of different sets of homologous external standards. The method was successfully applied to the semi-quantification of chemokines in human liver specimens and should be useful in further studies on steady state mRNA levels of C-C chemokines from low cell numbers or small tissue specimens.

Published
2000

152. Vitamins

Author

Manfred Eggersdorfer, Geo Adam, Michael John, Wolfgang Hähnlein, Ludvik Labler, Kai-U. Baldenius, Linda von dem Bussche-Hünnefeld, Eckhard Hilgemann, Peter Hoppe, Rainer Stürmer, Fritz Weber, August Rüttimann, Gérard Moine, Hans-Peter Hohmann, Roland Kurth, Joachim Paust, Horst Pauling, Bernd-Jürgen Weimann, Bruno Kaesler, Bernd Oster, Ulrich Fechtel, Klaus Kaiser, Bernd de Potzolli, Michael Casutt, Thomas Koppe, Michael Schwarz, Urs Hengartner, Antoine de Saizieu, Christof Wehrli, and René Blum

Published
2000
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153. Indigenous High Forests in the Transvaal with Special Reference to Inventory and Monitoring Systems

Author

G. R. von dem Bussche

Subjects
geography, geography.geographical_feature_category, Indigenous forest, Forestry, Monitoring system, Plant Science, Escarpment, State forest, Indigenous, Floristics
Abstract

SYNOPSIS The indigenous forests in the Transvaal comprise a total area of 35 385 ha, of which 18 088 ha or 51% are situated on State forest land. The forests are found mainly along the Drakensberg escarpment and, to a lesser extent, along the Soutpansberg. While Cooper (1985) carried out a comprehensive survey of all forests, other floristic surveys have been restricted to only a few forests. The necessity to implement monitoring system is emphasised.

Published
1991
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154. Gentechnik und Öffentlichkeit

Author

L. von dem Bussche-Hünnefeld and H. J. Bremme

Abstract

Wer annimmt, das Thema Wahrnehmung und Behandlung der Gentechnik in der Offentlichkeit sei kurz und einfach abzuhandeln, wird — bei auch nur etwas intensiverer Beschaftigung mit der Materie — schnell eines Besseren belehrt werden. Nicht nur das Interesse einer breiten Offentlichkeit an diesem Thema ist gros, sondern es beeindruckt und beschaftigt besonders die differenzierte Art der Wahrnehmung der Gentechnologie, und eine Menge an Fragen knupfen sich fur den Betrachter daran.

Published
1999
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158. Prolonged seizures recruit caudal subventricular zone glial progenitors into the injured hippocampus

Author

Department of Neurology, University of Michigan Medical Center, Ann Arbor, Michigan ; Department of Neurology, University of Michigan Medical Center, 4412E Kresge III Building, 200 Zina Pitcher Place, Ann Arbor, MI 48109-0585, Department of Neurology, University of California, San Francisco, California, Parent, Jack M., Von dem Bussche, Nicolas, Lowenstein, Daniel H., Department of Neurology, University of Michigan Medical Center, Ann Arbor, Michigan ; Department of Neurology, University of Michigan Medical Center, 4412E Kresge III Building, 200 Zina Pitcher Place, Ann Arbor, MI 48109-0585, Department of Neurology, University of California, San Francisco, California, Parent, Jack M., Von dem Bussche, Nicolas, and Lowenstein, Daniel H.

Abstract

Neurogenesis persists in the adult rat rostral forebrain subventricular zone (SVZ) and is stimulated by status epilepticus (SE). More caudal SVZ (cSVZ) neural progenitors migrate to the hippocampus after ischemic injury and contribute to CA1 pyramidal cell regeneration. Because SE also damages the hippocampus, we examined the effects of SE on cSVZ precursors. SE was induced in adult rats with pilocarpine, and cell proliferation in cSVZ and hippocampus was examined by bromodeoxyuridine (BrdU) and retroviral reporter labeling. Neural precursors were assayed by immunostaining for specfic markers between 1 and 35 days after SE. BrdU-positive cells labeled prior to SE markedly increased in numbers within 1-2 weeks in the cSVZ and infracallosal region, but not in the corpus callosum. Doublecortin-, polysialic acid neural cell adhesion molecule-, and TUC-4 (TOAD/Ulip/CRMP family-4)-immunostained cells with migrating morphology increased with a similar time course after SE and extended from the cSVZ to CA1 and CA3 regions. Retroviral reporters injected into the cSVZ of controls showed labeled cells with oligodendroglial morphology located in the cSVZ and corpus callosum; when injected 2 days prior to SE, many more reporter-labeled cells appeared several weeks later and were located in the cSVZ, corpus callosum, and hippocampus. Labeled cells showed glial morphologies and expressed astrocyte or oligodendrocyte markers. Neither BrdU- nor retroviral reporter-labeled cells coexpressed neuronal markers in controls or pilocarpine-treated rats. These results indicate that SE increases cSVZ gliogenesis and attracts newly generated glia to regions of hippocampal damage. Further study of seizure-induced gliogenesis may provide insight into mechanisms of adult neural progenitor regulation and epileptogenesis. © 2006 Wiley-Liss, Inc.

Published
2007

159. Nanomechanical mechanism for lipid bilayer damage induced by carbon nanotubes confined in intracellular vesicles.

Author

Wenpeng Zhu, von dem Bussche, Annette, Xin Yi, Yang Qiu, Zhongying Wang, Weston, Paula, Hurt, Robert H., Kane, Agnes B., and Huajian Gao

Subjects
*BILAYER lipid membranes, *CARBON nanotubes, *CELL-mediated cytotoxicity, *MOLECULAR dynamics, *LYSOSOMES
Abstract

Understanding the behavior of low-dimensional nanomaterials confined in intracellular vesicles has been limited by the resolution of bioimaging techniques and the complex nature of the problem. Recent studies report that long, stiff carbon nanotubes are more cytotoxic than flexible varieties, but the mechanistic link between stiffness and cytotoxicity is not understood. Here we combine analytical modeling, molecular dynamics simulations, and in vitro intracellular imaging methods to reveal 1D carbon nanotube behavior within intracellular vesicles. We show that stiff nanotubes beyond a critical length are compressed by lysosomal membranes causing persistent tip contact with the inner membrane leaflet, leading to lipid extraction, lysosomal permeabilization, release of cathepsin B (a lysosomal protease) into the cytoplasm, and cell death. The precise material parameters needed to activate this unique mechanical pathway of nanomaterials interaction with intracellular vesicles were identified through coupled modeling, simulation, and experimental studies on carbon nanomaterials with wide variation in size, shape, and stiffness, leading to a generalized classification diagram for 1D nanocarbons that distinguishes pathogenic from biocompatible varieties based on a nanomechanical buckling criterion. For a wide variety of other 1D material classes (metal, oxide, polymer), this generalized classification diagram shows a critical threshold in length/width space that represents a transition from biologically soft to stiff, and thus identifies the important subset of all 1D materials with the potential to induce lysosomal permeability by the nanomechanical mechanism under investigation. [ABSTRACT FROM AUTHOR]

Published
2016
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160. Chemical Dissolution Pathways of MoS2 Nanosheets in Biological and Environmental Media.

Author

Zhongying Wang, von dem Bussche, Annette, Yang Qiu, Valentin, Thomas M., Kyle Gion, Kane, Agnes B., and Hurt, Robert H.

Subjects
*DISSOLUTION (Chemistry), *SOLUTION (Chemistry), *AQUEOUS solutions, *OXIDATION, *MOLYBDATES
Abstract

Material stability and dissolution in aqueous media are key issues to address in the development of a new nanomaterial intended for technological application. Dissolution phenomena affect biological and environmental persistence; fate, transport, and biokinetics; device and product stability; and toxicity pathways and mechanisms. This article shows that MoS2 nanosheets are thermodynamically and kinetically unstable to O2-oxidation under ambient conditions in a variety of aqueous media. The oxidation is accompanied by nanosheet degradation and release of soluble molybdenum and sulfur species, and generates protons that can colloidally destabilize the remaining sheets. The oxidation kinetics are pH-dependent, and a kinetic law is developed for use in biokinetic and environmental fate modeling. MoS2 nanosheets fabricated by chemical exfoliation with n-butyl-lithium are a mixture of 1T (primary) and 2H (secondary) phases and oxidize rapidly with a typical half-life of 1-30 days. Ultrasonically exfoliated sheets are in pure 2H phase, and oxidize much more slowly. Cytotoxicity experiments on MoS2 nanosheets and molybdate ion controls reveal the relative roles of the nanosheet and soluble fractions in the biological response. These results indicate that MoS2 nanosheets will not show long-term persistence in living systems and oxic natural waters, with important implications for biomedical applications and environmental risk. [ABSTRACT FROM AUTHOR]

Published
2016
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161. Editorial.

Author

Alenfelder, Heinz, von dem Bussche, Axel Freiherr, Klabunde, Achim, Mannah, Sakyi, Schmalenberger, Alexander, Schrems, Maximilian „Max“, and Weichert, Thilo

Published
2023

173. Not so creepy crawler

Author

von dem Bussche, Franziska, primary, Weiand, Klara, additional, Linse, Benedikt, additional, Furche, Tim, additional, and Bry, François, additional

Published
2010
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175. Corrigendum to: Hepatitis C virus NS2 protein triggers endoplasmic reticulum stress and suppresses its own viral replication [J Hepatol. 2010 Nov;53(5):797–804]

Author

Annette von dem Bussche, Jack R. Wands, Ke Li, Amrin Khander, Jianguo Wang, Takaji Wakita, Gideon S Loevinsohn, Raiki Machida, and Jisu Li

Subjects
Hepatology, Viral replication, Endoplasmic reticulum, Hepatitis C virus, medicine, Biology, medicine.disease_cause, Virology, Article
Published
2011
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190. Landwirtschaft im Aufwand!

Author

Freiherr von dem Bussche, P. and Freiherr von dem Bussche, P.

Abstract

Via 6 thesen wordt het bewustzijn van de eigen Duitse concurrentiepositie op de internationale markt gestimuleerd

Published
1996

191. Bowl Shaped Cavitands Dimerize and Complex Certain Organic Guests in Organic Solvents which Themselves are Poor Guests

Author

Christoph von dem Bussche-Hünnefeld, Roger C. Helgeson, Dirk Bühring, Carolyn B. Knobler, Donald J. Cram, Christoph von dem Bussche-Hünnefeld, Roger C. Helgeson, Dirk Bühring, Carolyn B. Knobler, and Donald J. Cram

Abstract

The syntheses and binding properties of rigidly bowl-shaped polycyclic cavitands (1—4) are reported. Attached to the four aryl rim positions of the bowls are four benzenes substituted in their para positions with four CC^Me, Br, OH or NO2 groups, which deepen the bowls. Attached to the base of the bowls are four pentyl feet, which increase the solubilities of these hosts in organic solvents. Of the four hosts, only the one containing the CO2Me groups dimerized both in the crystalline state and in solution in ten deute- rated solvents which themselves are poor guests. In three other deuterated solvents, no dimerization was observed. A crystal structure of the dimer showed that one p-MeC^CCs^ group of each monomer occupied the cavity of its complexing partner in a reciprocating double host-guest arrangement. Such a structure is compatible with the ^H-NMR spectra of the dimer in solution. The dimer was also detected in its FAB-MS. The tetrabromocavitand at low concentrations in CD2CI2 complexed MeCC^C^Me, MeCC^Me, PhCC^Me and MeCOC^CC^Me. Tetranitrocavitand 4 also complexed MeC02CH2Me in CD2CI2 as solvent.

Published
1996

194. Vitamins

Author

Eggersdorfer, Manfred, primary, Adam, Geo, additional, John, Michael, additional, Hähnlein, Wolfgang, additional, Labler, Ludvik, additional, Baldenius, Kai-U., additional, von dem Bussche-Hünnefeld, Linda, additional, Hilgemann, Eckhard, additional, Hoppe, Peter, additional, Stürmer, Rainer, additional, Weber, Fritz, additional, Rüttimann, August, additional, Moine, Gérard, additional, Hohmann, Hans-Peter, additional, Kurth, Roland, additional, Paust, Joachim, additional, Pauling, Horst, additional, Weimann, Bernd-Jürgen, additional, Kaesler, Bruno, additional, Oster, Bernd, additional, Fechtel, Ulrich, additional, Kaiser, Klaus, additional, de Potzolli, Bernd, additional, Casutt, Michael, additional, Koppe, Thomas, additional, Schwarz, Michael, additional, Hengartner, Urs, additional, de Saizieu, Antoine, additional, Wehrli, Christof, additional, and Blum, René, additional

Published
2000
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196. Vicarious Nucleophilic Aromatic Substitution via Trapping of an .alpha.-Ketosulfonium Ion Generated by Pummerer-Type Rearrangement of 2-(Phenylsulfinyl)phenols: Preparation of Biaryls

Author

Linda von dem Bussche, Michael E. Jung, and Chung Kim

Subjects
chemistry.chemical_classification, chemistry.chemical_compound, chemistry, Nucleophilic aromatic substitution, Yield (chemistry), Pummerer rearrangement, Organic Chemistry, Nucleophilic substitution, Salt (chemistry), Regioselectivity, Phenols, Electrophilic aromatic substitution, Medicinal chemistry
Abstract

A new method for the nucleophilic substitution of phenols at the ortho position has been developed which uses as the key step the trapping of an α-ketosulfonium salt generated by a Pummerer rearrangement of 2-(phe- nylsulfinyl)phenols. In addition to preparing 2-halo- and 2-(acyloxy)- phenols, this «vicarious substitution» also allows the preparation of biaryls in fair yield

Published
1994
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