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Naturally occurring substances and their derivatives function as vital resources for pesticides that can be used in fields, such as insecticide production and fungicide development. As a botanical entity displaying multifaceted biological functions, wormwood has received thorough scrutiny across multiple sectors. The insect repellency potency combined with antibacterial and antifungal activities of wormwood position it as a potential candidate for prospective development into eco-friendly chemical pesticides. In this research, Wormwood essential oil was procured via ethanol water under ultrasonic scenarios and subsequently diluted with PEG 400 to formulate green chemical pesticides. The defensive efficacy of this green pesticide on plants was validated through 2 weeks of clustered plant growth experiments. Active constituents that exerted their effects were scrutinized by GC-MS. Furthermore, this green pesticide also displays efficacious effects on the prevention and management of aphids, exhibiting a dose-dependent relationship. 4-terpenol, eucalyptol, carvacrol, and L-borneol were identified by GC-MS as the predominant active constituents in this green chemical pesticide. Wormwood can be leveraged to develop green chemical pesticides, which can protect plants without contaminating the environment. [ABSTRACT FROM AUTHOR]

Triazoles are compounds with various biological activities, including fungicidal action. They became popular through cholinesterase studies after the successful synthesis of the dual binding femtomolar triazole inhibitor of acetylcholinesterase (AChE, EC 3.1.1.7) by Sharpless et al. via in situ click chemistry. Here, we evaluate the anticholinesterase effect of the first isopropanol triazole fungicide mefentrifluconazole (Ravystar®), developed to overcome fungus resistance in plant disease management. Mefentrifluconazole is commercially available individually or in a binary fungicidal mixture, i.e., with pyraclostrobin (Ravycare®). Pyraclostrobin is a carbamate that contains a pyrazole ring. Carbamates are known inhibitors of cholinesterases and the carbamate rivastigmine is already in use for the treatment of Alzheimer's disease. We tested the type and potency of anticholinesterase activity of mefentrifluconazole and pyraclostrobin. Mefentrifluconazole reversibly inhibited human AChE and BChE with a seven-fold higher potency toward AChE (Ki = 101 ± 19 μM). Pyraclostrobin (50 μM) inhibited AChE and BChE progressively with rate constants of (t1/2 = 2.1 min; ki = 6.6 × 103 M−1 min−1) and (t1/2 = 1.5 min; ki = 9.2 × 103 M−1 min−1), respectively. A molecular docking study indicated key interactions between the tested fungicides and residues of the lipophilic active site of AChE and BChE. Additionally, the physicochemical properties of the tested fungicides were compared to values for CNS-active drugs to estimate the blood–brain barrier permeability. Our results can be applied in the design of new molecules with a lesser impact on humans and the environment. [ABSTRACT FROM AUTHOR]

Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R2) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices. [ABSTRACT FROM AUTHOR]

The 2-pyrone moiety is present in a wide range of structurally diverse natural products with various biological activities. The plant biosynthetic routes towards these compounds mainly depend on the activity of either type III polyketide synthase-like 2-pyrone synthases or hydroxylating 2-oxoglutarate dependent dioxygenases. In the present study, the substrate specificity of these enzymes is investigated by a systematic screening using both natural and artificial substrates with the aims of efficiently forming (new) products and understanding the underlying catalytic mechanisms. In this framework, we focused on the in vitro functional characterization of a 2-pyrone synthase Gh2PS2 from Gerbera x hybrida and two dioxygenases AtF6'H1 and AtF6'H2 from Arabidopsis thaliana using a set of twenty aromatic and aliphatic CoA esters as substrates. UHPLC-ESI-HRMSn based analyses of reaction intermediates and products revealed a broad substrate specificity of the enzymes, enabling the facile "green" synthesis of this important class of natural products and derivatives in a one-step/one-pot reaction in aqueous environment without the need for halogenated or metal reagents and protective groups. Using protein modeling and substrate docking we identified amino acid residues that seem to be important for the observed product scope. • Enzymatic synthesis led to the one-pot formation of 2-pyrones from coenzyme A esters. • Promiscuous polyketide synthases or hydroxylases were applied in the reactions. • The syntheses yielded both natural and new-to-nature 2-pyrones including coumarins. • The formed derivatives constitute lead structures for improved aromas and biocides. [ABSTRACT FROM AUTHOR]

Purpose: This work explores the dynamics of spatiotemporal changes in the taxonomic structure of biofilms and the degradation kinetics of three imidazole group compounds: carbendazim (CBZ), methyl thiophanate (MT), and benomyl (BN) by a multispecies microbial community attached to a fixed bed horizontal tubular reactor (HTR). This bioreactor mimics a permeable reactive biobarrier, which helps prevent the contamination of water bodies by pesticides in agricultural wastewater. Methods: To rapidly quantify the microbial response to crescent loading rates of benzimidazole compounds, a gradient system was used to transiently raise the fungicide volumetric loading rates, measuring the structural and functional dynamics response of a microbial community in terms of the volumetric removal rates of the HTR entering pollutants. Results: The loading rate gradient of benzimidazole compounds severely impacts the spatiotemporal taxonomic structure of the HTR biofilm-forming microbial community. Notable differences with the original structure in HTR stable conditions can be noted after three historical contingencies (CBZ, MT, and BN gradient loading rates). It was evidenced that the microbial community did not return to the composition prior to environmental disturbances; however, the functional similarity of microbial communities after steady state reestablishment was observed. Conclusions: The usefulness of the method of gradual delivery of potentially toxic agents for a microbial community immobilized in a tubular biofilm reactor was shown since its functional and structural dynamics were quickly evaluated in response to fungicide composition and concentration changes. The rapid adjustment of the contaminants' removal rates indicates that even with changes in the taxonomic structure of a microbial community, its functional redundancy favors its adjustment to gradual environmental disturbances. [ABSTRACT FROM AUTHOR]

The increased consumption of pesticides can have a negative environmental impact by increasing the essential metals to toxic levels. Bordasul® is a commonly used fungicide in Brazil and it is composed of 20% Cu, 10% sulfur, and 3.0% calcium. The study of fungicides in vivo in non-target model organisms can predict their environmental impact more broadly. The Drosophila melanogaster is a unique model due to its ease of handling and maintenance. Here, the potential toxicity of Bordasul® was investigated by assessing the development, survival, and behavior of exposed flies. Exposure to Bordasul® impaired the development (p < 0.01) and caused a significant reduction in memory retention (p < 0.05) and locomotor ability (p < 0.001). Fungicides are needed to assure the world's food demand; however, Bordasul® was highly toxic to D. melanogaster. Therefore, Bordasul® may be potentially toxic to non-target invertebrates and new environmentally-safe biofertilizers have to be developed to preserve the biota. [ABSTRACT FROM AUTHOR]

The cultivated apple (Malus × domestica) is one of Türkiye's most important economic crops. Over the growing seasons of 2020–2022, typical symptoms of branch cankers and scaling-off of the bark on apple trees were observed in the Eğirdir district of Isparta province. Botryosphaeriaceae fungi were consistently isolated from symptomatic branches. The fungal pathogen was identified as Diplodia bulgarica based on cultural and morphological characteristics and ITS region of DNA sequence data comparisons. Pathogenicity tests were performed on detached shoots by inoculating them with mycelial plugs. Diplodia bulgarica isolates caused necrotic lesions on Scarlet Spur apple detached shoots. To aid in the development of control strategies for this fungus, nine fungicides were examined in vitro for their antifungal effects against the pathogen at different concentrations. Chlorothalonil, tebuconazole, thiophanate-methyl, and fluazinam were the most efficient fungicides, with EC50 values were <1.0 µg/mL. [ABSTRACT FROM AUTHOR]

Cumin (Cuminum cyminum L.) is an important seed spice crop at world level and India is the leading producer (70% of world production), consumer and exporter of cumin in the world. Almost 80% of the crop cultivated is consumed in India. The crop yield is affected by several biotic and abiotic factors, among them, blight caused by Alternaria burnsii (Uppal, Patel & Kamat) is one of the most dreaded diseases and a major production constrain for the successful cultivation of cumin crop. Among the non-systemic fungicides tested, captan 75% WP, chlorothalonil 75 % WP and mancozeb 75 % WP were most effective with 95.67, 95.62 and 94.86 per cent growth inhibition, respectively whereas hexaconazole 5 % EC was most effective in systemic fungicides with 99.98 per cent inhibition followed by Tebuconazole 25.9 % EC 87.40 per cent inhibition. [ABSTRACT FROM AUTHOR]

Alternaria blight is one of the important diseases of cumin. The disease initiation was observed at nearly one month age of the crop during warm humid weather in the epidemic form at flowering stage and seeds could not mature to full size. The pathogen was isolated by tissue isolation technique. Pathogenicity test was proved by following Koch's postulates. The pathogen was identified as Alternaria burnsii based on morphological characters and ITS sequencing. The sequence was submitted to gene bank NCBI and it was accepted by NCBI with accession number MT856660. For effective control of cumin blight with fungicides a field experiment was conducted. Efficacy of eight fungicides was tested under field condition for disease intensity and yield of cumin. Among the eight combined and single fungicides, Azoxystrobin 11% + Tebuconazole 18.30% SC at concentration 0.044% was most effective with 14.76% least mean disease intensity and highest yield of 907 kg/ha followed by Metiram 55% + Pyraclostrobin 5 % WG at concentration 0.18% with 18.27% least mean disease intensity and yield of 889 kg/ha. [ABSTRACT FROM AUTHOR]

This study investigated the relationship between yield, thousand kernel weight (TKW), and crude protein of soft white winter wheat–club variety (Barbee) and soft white winter wheat common variety (Zvezdana) susceptible to leaf rust and powdery mildew under different cultivation practices. Results revealed divergence in associations between yield, TKW, and crude protein loss of winter wheat varieties susceptible to obligate pathogens. Under the same level of leaf rust infection, N-input limited yield loss of the two varieties but not to the same extent. TKW loss was affected only by variety×cultivation practice and was significantly correlated with yield loss (r = −0.727, p = 0.011) and crude protein loss (r = −0.600, p = 0.05) only in club winter wheat. We suspected that Ninput affects the difference in the relationship between yield and TKW loss among varieties. Crude protein and yield loss had a low association (R2 = 18%, p = 0.05). Finally, this study indicated that more attention should be paid to the determination of pathogen pressure that triggers yield loss. It also pointed out that yield, TKW, and crude protein response to fungicides could differ in susceptible varieties. The contribution of fungicide to yield enhancement was highly associated with the specific reaction of the variety to pathogen infection rather than solely the disease level itself. [ABSTRACT FROM AUTHOR]

The pathogenic fungus Batrachochytrium dendrobatidis has caused declines of amphibians worldwide. Yet our understanding of how water quality influences fungal pathogenicity is limited. Here, we reviewed experimental studies on the effect of water quality on this pathogen to determine which parameters impacted disease dynamics consistently. The strongest evidence for protective effects is salinity which shows strong antifungal properties in hosts at natural levels. Although many fungicides had detrimental effects on the fungal pathogen in vitro, their impact on the host is variable and they can worsen infection outcomes. However, one fungicide, epoxiconazole, reduced disease effects experimentally and likely in the field. While heavy metals are frequently studied, there is weak evidence that they influence infection outcomes. Nitrogen and phosphorous do not appear to impact pathogen growth or infection in the amphibian host. The effects of other chemicals, like pesticides and disinfectants on infection were mostly unclear with mixed results or lacking an in vivo component. Our study shows that water chemistry does impact disease dynamics, but the effects of specific parameters require more investigation. Improving our understanding of how water chemistry influences disease dynamics will help predict the impact of chytridiomycosis, especially in amphibian populations affected by land use changes. [ABSTRACT FROM AUTHOR]

Plant pathogenic fungi pose a major threat to global food security, ecosystem services, and human livelihoods. Effective and broad-spectrum fungicides are needed to combat these pathogens. In this study, a novel antifungal 2-oxyacetate hydrazide quinoxaline scaffold as a simple analogue was designed and synthesized. Their antifungal activities were evaluated against Botrytis cinerea (B. cinerea), Altemaria solani (A. solani), Gibberella zeae (G. zeae), Rhizoctonia solani (R. solani), Colletotrichum orbiculare (C. orbiculare), and Alternaria alternata (A. alternata). These results demonstrated that most compounds exhibited remarkable inhibitory activities and possessed better efficacy than ridylbacterin, such as compound 15 (EC50 = 0.87 μg/mL against G. zeae, EC50 = 1.01 μg/mL against C. orbiculare) and compound 1 (EC50 = 1.54 μg/mL against A. alternata, EC50 = 0.20 μg/mL against R. solani). The 3D-QSAR analysis of quinoxaline-2-oxyacetate hydrazide derivatives has provided new insights into the design and optimization of novel antifungal drug molecules based on quinoxaline. [ABSTRACT FROM AUTHOR]

Microbes and enzymes play essential roles in soil and plant rhizosphere ecosystem functioning. However, fungicides and plant root secretions may impact the diversity and abundance of microbiota structure and enzymatic activities in the plant rhizosphere. In this study, we analyzed soil samples from the rhizosphere of four cannabinoid-rich hemp (Cannabis sativa) cultivars (Otto II, BaOx, Cherry Citrus, and Wife) subjected to three different treatments (natural infection, fungal inoculation, and fungicide treatment). DNA was extracted from the soil samples, 16S rDNA was sequenced, and data were analyzed for diversity and abundance among different fungicide treatments and hemp cultivars. Fungicide treatment significantly impacted the diversity and abundance of the hemp rhizosphere microbiota structure, and it substantially increased the abundance of the phyla Archaea and Rokubacteria. However, the abundances of the phyla Pseudomonadota and Gemmatimonadetes were substantially decreased in treatments with fungicides compared to those without fungicides in the four hemp cultivars. In addition, the diversity and abundance of the rhizosphere microbiota structure were influenced by hemp cultivars. The influence of Cherry Citrus on the diversity and abundance of the hemp rhizosphere microbiota structure was less compared to the other three hemp cultivars (Otto II, BaOx, and Wife). Moreover, fungicide treatment affected enzymatic activities in the hemp rhizosphere. The application of fungicides significantly decreased enzyme abundance in the rhizosphere of all four hemp cultivars. Enzymes such as dehydrogenase, dioxygenase, hydrolase, transferase, oxidase, carboxylase, and peptidase significantly decreased in all the four hemp rhizosphere treated with fungicides compared to those not treated. These enzymes may be involved in the function of metabolizing organic matter and degrading xenobiotics. The ecological significance of these findings lies in the recognition that fungicides impact enzymes, microbiota structure, and the overall ecosystem within the hemp rhizosphere. [ABSTRACT FROM AUTHOR]

White rot, caused by the fungus Coniella diplodiella, is an important but poorly studied disease that mainly affects grapevine clusters. White rot control typically involves the repeated application of fungicides, which may be unjustified in some cases given that the key period of berry susceptibility to infection remains unclear and controversial. In this study, germination of C. diplodiella conidia and mycelium growth were investigated on water agar (conidial germination only) and artificial media similar to berry juice at five growth stages from pea‐sized to berries ripe for harvest. On water agar, conidia germinated from 10°C to 35°C (with an optimum of 20–30°C) with >2 h of moisture incubation. Both conidial germination and mycelial growth were higher on agar similar to berry juice at véraison to berry softening than at other stages, with the lowest values detected for the ripe berries. The berries were also artificially inoculated with conidia at the different growth stages, showing that grape clusters were susceptible to infection for a long period, albeit with varying degrees of susceptibility at different stages. This implies that effective disease control requires interventions from early berry development, whenever weather conditions are conducive to infection. Further development of a predictive model accounting for weather conditions and berry susceptibility dynamics would facilitate the dynamic estimation of the disease risk during the grapevine‐growing season and contribute to a risk‐based application of fungicides for white rot control. [ABSTRACT FROM AUTHOR]

Leaf decomposition is a key process in stream ecosystems within forested catchments; it is driven by microbial communities, particularly fungi and bacteria. These microorganisms make nutrients and energy bound in leaves available for wider parts of the food web. Leaf‐associated microorganisms are subjected to anthropogenic pressures, such as the increased exposure to nutrients and fungicides associated with land‐use change. We assessed the sensitivity of leaf‐associated microbial communities with differing exposure histories, namely, from pristine (P) streams, and streams impacted by wastewater (W) and agricultural run‐off (vineyards; V). In the laboratory, microbial communities were exposed to elevated nutrient (NO3‐N: 0.2–18.0 mg/L, PO4‐P: 0.02–1.8 mg/L) and fungicide concentrations (sum concentration 0–300 µg/L) in a fully crossed 3 × 4 × 4‐factorial design over 21 days. Leaf decomposition and exoenzyme activity were measured as functional endpoints, and fungal community composition and microbial abundance served as structural variables. Overall, leaf decomposition did not differ between fungicide treatments or exposure histories. Nonetheless, substantial changes in the fungal community composition were observed after exposure to environmentally relevant fungicide concentrations. Elevated nutrient concentrations assisted leaf decomposition, and the effect size depended on the exposure history. The observed changes in the fungal community composition support the principle of functional redundancy, with highly efficient decomposers maintaining leaf decomposition. Environ Toxicol Chem 2024;43:1300–1311. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. [ABSTRACT FROM AUTHOR]

One of the commonest pesticides used in agriculture is the fungicide carbendazim, which can cause deleterious effects on the physiology and behaviour of acutely and chronically exposed animals. However, few studies have evaluated the effects of carbendazim on fish behaviour and our main objective was to evaluate behavioural changes on adult zebrafish (Danio rerio) exposed to this fungicide. We studied 177 fishes, divided into control groups (not exposed) and treatment groups, exposed to a concentration of 120 µg/L for 7, 14, 21 and 28. Behavioural data were collected using the scan sampling, with instantaneous recording every 30 s. We evaluated and compared fish behaviours and aquarium occupancy between treatments and controls using Generalized Linear Mixed Models. The results indicate that carbendazim initially elicited slow swimming and later fast swimming, decreased aggressiveness, and caused behavioural changes indicative of distress, like abnormal swimming and the gulping of air in water surface. In the wild, this behavioural change can result in an increased predation risk and consequent decrease or extinction of populations, revealing important ecological issues for animal communities living in polluted water bodies. [ABSTRACT FROM AUTHOR]

The use of natural products in the control of fungal diseases in plants is considered an alternative to synthetic fungicides due to their less negative effects on the environment. In this study, in vitro inhibitory effect of plant essential oils (PEOs) of black cumin, cumin, chamomile, cedarwood, and ginger were investigated for controlling two species of Alternaria, including Alternari solani and Alternaria alternata on tomato and cabbage under in vitro conditions, respectively. Aiming to evaluate the mycelial growth of the pathogen, mycelial discs were placed in Petri plates with 0, 500, 1000, 1500, 2000, and 2500 µL/L of PEOs. The experiment was carried out in a randomized plot design with three replications. Chemical analysis of PEO components were determined by Gas Chromatography and Mass Spectrometry methods. A total of 69 chemical compounds were determined in five different PEOs. As the main chemical compounds, Cuminaldehyde was detected in cumin PEO, Sesquithujene was found in ginger PEO, and Eucalyptol (1,8-cineole) was determined in black cumin, chamomile, and cedarwood PEOs. All five PEOs were found to inhibit the growth of Alternaria species in a dose-dependent manner, whereas cumin EO was determined more inhibitory effect against A. solani and A. alternata. Cumin PEO showed the highest effect against Alternaria species because it contains a Cuminaldehyde chemical compound. The lowest inhibition percentage was found in chamomile PEO compared to other PEOs. This study suggested that cumin PEO has the potential as an antifungal agent for controlling of Alternaria diseases. [ABSTRACT FROM AUTHOR]

Zebrafish being the best animal model to study, every attempt has been made to decipher the toxic mechanism of every fungicide of usage and interest. It is important to understand the multiple targets of a toxicant to estimate the toxic potential in its totality. A total of 22 fungicides of different classes like amisulbrom, azoxystrobin, carbendazim, carboxin, chlorothalonil, difenoconazole, etridiazole, flusilazole, fluxapyroxad, hexaconazole, kresoxim methyl, mancozeb, myclobutanil, prochloraz, propiconazole, propineb, pyraclostrobin, tebuconazole, thiophanate-methyl, thiram, trifloxystrobin and ziram were reviewed and analyzed for their multiple explored targets in zebrafish. Toxic end points in zebrafish are highly informative when it comes to network analysis. They provide a window into the molecular and cellular pathways that are affected by a certain toxin. This can then be used to gain insights into the underlying mechanisms of toxicity and to draw conclusions on the potential of a particular compound to induce toxicity. This knowledge can then be used to inform decisions about drug development, environmental regulation, and other areas of research. In addition, the use of zebrafish toxic end points can also be used to better understand the effects of environmental pollutants on ecosystems. By understanding the pathways affected by a given toxin, researchers can determine how pollutants may interact with the environment and how this could lead to health or environmental impacts. [ABSTRACT FROM AUTHOR]

Loam examples were gathered through the 2020-2021 rising periods, and the following measurements were made: Viable bacterial count by reducing root colonization. The outcomes of reviewing the impact of the fungicide Benomyl on development and viable microflora count revealed that the highest microbial count was in Al-Madaein 80 x10³ CFU/mL was recorded ., and the lowest count was 60 x10³ CFU/mL for the Aushtar area, The microbial viable count values for the affected microorganisms with Benomyl were decrease to 27x10³ and 65 x 10³ CFU/mL respectively. Those consequences specify that Benomyl has a robust choosiness contrary to microflora, especially when compared to the benomyl effect as folded dose, the microflora l count decreases to 25 x10³ CFU /mL in the Aushtar area and increases to 60 x10³ CFU/mL in Al-Madaein area. Whereas the study estimated the level of eight elements in soil (Mn, Fe, Cu, Zn, NO3, P, K, and NH4) cultured with Cyperus rotundus L. Which mentioned the effect of benomyl on these levels after three days of treatment. Mn concentration ranged between 5.96 to 9.11 ppm, while after fungicide benomyl, it decreased to 5.63 -6.53 ppm similar results were observed for other elements. The highest affected element was Mn in the Aushtar area. Those consequences designate that Benomyl has a stout fussiness in contrast to soil nutrients. The greatness of benomyl impacts on loam ingredients and procedures were minor, qualified to impact on mycorrhizal root foundation (reduction through benomyl). [ABSTRACT FROM AUTHOR]

The peculiarities of the primary visual inspection of winter wheat seeds are considered. In the North-East of Ukraine, three groups of abnormal seeds (wrinkled, small seeds, and with a black point) were being identified by macroanalysis during 2017-2022. Factors affecting the representation of these groups in the samples were analyzed. It was determined that the amount of abnormal seeds is determined by the growing conditions of wheat, the place of cultivation and spraying of wheat with fungicides. The maximum number of substandard seeds was noted in Polissia, where black point dominated. In the Forest-steppe, the largest amount of such seeds was recorded in 2022, among which small seeds prevailed. A study of the characteristics of the manifestation of a black point showed that the blackening of the embryo occurs only from above. A seed with a black point is usually mature. For the first time, it was proven that spraying plants with essential oils of cinnamon and fennel leads to an increase in the appearance of the black point, which once again confirms its abiotic nature. The study of the germination of externally healthy and abnormal seeds of the Bohdana variety of the 2018 and 2019 harvests under conditions of low temperature and over moistening showed better indicators for the first group. But the germination of abnormal seeds had contradictory results, especially in different years. Comparison of the germination and development of seedlings from healthy and blackening seeds under favorable conditions during different periods of storage of wheat samples, from different places of cultivation, on different genotypes gave new unexpected results: higher indicators in the variant with black point. During further study in conditions of low temperature, which is explained by very late sowing of winter wheat in recent years in Ukraine, completely different data were obtained. Seed germination was better in healthy seeds. Seedlings developed faster at first also from healthy seeds, but on the last two dates of measurement, they began to grow faster from seeds with black point. [ABSTRACT FROM AUTHOR]

Mango fruits' quality, marketability, and shelf life are significantly influenced by postharvest decay. Mango fruit disease development during storage is inhibited by fungicides. The objective of this investigation was to assess the relative effectiveness of two fungicides, azoxystrobin 18.2% + difenoconazole 11.4% SC and hexaconazole 5% SC, in mitigating postharvest deterioration of mango cv. 'Langra' during low temperature storage. Three pathogens were found associated with postharvest decay of mango, i.e. Colletotrichum gloeosporioides, Alternaria alternata , and Penicillium expansum. In comparison to untreated ones, the preharvest application of fungicide treatments provided noticeably better disease control. The foliar application of azoxystrobin 18.2% + difenoconazole 11.4% SC (0.1%) 15 days before the harvesting of fruits significantly improved fruit firmness, soluble solid content, titratable acidity, reduced the disease incidence, percentage disease index, and lesion diameter followed by the application of hexaconazole 5% SC (0.1%) compared to the control. Furthermore, it was found that pre-harvest fungicide spraying of azoxystrobin 18.2% + difenoconazole 11.4% SC (0.1%) was an effective treatment for lowering postharvest deterioration and enhancing the physio-chemical characteristics of mango fruits during low-temperature storage. [ABSTRACT FROM AUTHOR]

The long-term use of copper (Cu)-based fungicide sprays in orchards is associated with changes in soil Cu levels. However, there is a gap in knowledge regarding the potential accumulation of Cu in orchards and the associated impacts on the soil microbial structure. This study assessed the possibility of Cu accumulation in different avocado orchard farms and further evaluated the potential effect on soil microbial activities. Soil Cu levels were quantified in Tauranga and Northland, and three avocado orchards were analysed in each experimental location. All avocado farms in both sites received Cu-based fungicide sprays for over eight years. Soil samples were collected at a 0–20 cm depth from all six orchards. The soil total and bioavailable Cu, changes in soil chemical properties, microbial biomass, dehydrogenase activity, alkaline phosphatase activity, and acid phosphatase activity were measured. The results revealed that the total Cu and bioavailable Cu concentrations in Tauranga orchards were 81.3 and 0.32, 196.7 and 0.82, and 33.6 and 0.31 mg Cu kg−1 in Farms 1, 2, and 3, respectively. In Northland orchards, the total Cu and bioavailable Cu were 54.5 and 0.06, 18.4 and 0.77, and 46 and 0.34 mg Cu kg−1 in Farm 1, 2, and 3, respectively. Five out of six of the avocado orchard farms assessed in this study had total Cu concentrations greater than 30 mg Cu kg−1 reported in New Zealand native land. The magnitude of Cu accumulation was linked with soil pH and C content. No clear trend was observed between soil Cu concentrations and the soil microbial activity. Our study results demonstrated that the long-term use of Cu-based fungicide sprays can elevate Cu concentrations in orchard soils. Mitigation strategies need to be explored to abate the accumulation of Cu in orchard soils. [ABSTRACT FROM AUTHOR]

In this case study, the current situation faced by coffee growers attempting to control coffee leaf rust (Hemileia vastatrix) in Hawaii is reported. CLR is considered the most devastating disease affecting coffee crops worldwide and was detected in Hawaii in 2020. Three small coffee farms from the South Kona district of Hawaii Island were selected. The goals of this case study were to: (1) assist coffee growers in the early detection of CLR incidence, and consequently support farmers with recommendations for control, (2) record agronomic information and management practices, and (3) estimate the cost to control CLR during 2021 and 2022 seasons. Low CLR incidence (<4%) was initially observed at all farms (January–June 2021), but increased as the harvest began, ending the season (December 2021) at 77%, 21% and 6% incidence at farms 1, 2 and 3, respectively. At the end of 2022 season (December), CLR incidence reached 43%, 20% and 3% at farms 1, 2 and 3, respectively. The number of sprays per season (5–10), the type of fungicides applied (preventive, curative), the timing of sprays, the efficacy of applications and weather conditions all played a role in determining the infection rates at each farm. Effective control of CLR is possible in Hawaii if the sprays of fungicides are carried out with the right products, appropriate timing and good coverage. [ABSTRACT FROM AUTHOR]

Improved management strategies are needed to increase corn (Zea mays L.) production. This study aimed to determine suitable cultural practices for improved corn production in Mississippi. Two experiments were setup side‐by‐side (addition/deletion) at Verona and Stoneville, MS, from 2020 to 2022. A randomized complete block design was implemented that included two row configurations (single‐ and twin‐row), two plant populations (32,000 and 40,000 plants acre−1), and six combinations of nutrients with or without a fungicide. Nutrients including nitrogen (N) 210 and 280 lb acre−1, phosphorus (P) 40 lb acre−1, potassium (K) 100 lb acre−1, elemental sulfur (S) 20 lb acre−1, zinc (Zn) 10 lb acre−1, and fungicide at 3.72 oz acre−1 were applied. In the addition trial, nutrients plus fungicide were added incrementally, whereas in the deletion trial these were withheld in a stepwise manner. Among the tested factors, row configuration impacts were the most consistent among all site‐years; specifically, twin‐rows resulted in higher yield compared to single‐row. Additionally, higher plant population under irrigated conditions (Stoneville) resulted in greater yield compared to rainfed conditions (Verona). Higher rate of N and fungicide application affected grain yield positively, but these agronomic benefits were not economically feasible. This study determined that application of different nutrients can enhance the yield to a limited extent, and farmers should consider the economic investment of fertilizer and fungicides. Moreover, producers should balance yield and profit by taking soil testing and fertilizer prices into consideration. Plain Language Summary: Corn is the most consumed cereal, and its demand is increasing. Field experimentations are needed to continuously improve corn yield. We conducted field trials over 3 years at two locations in Mississippi. Corn yield was collected when planted at 32,000 or 40,000 plant population, on twin or single row, with application of several different fertilizers and fungicide. We found that twin‐rows always had higher yield compared to single‐row. Whenever irrigation was available, yield was higher with 40,000, and without irrigation, yield was higher in 32,000. With fertilizer application, applying higher rates of nitrogen and fungicide application increased grain yield. But when the price of fertilizer and fungicide are considered, the yield increase is not enough to make any profit to the producers. So, we found that even though there are advantages of fertilizer application, farmers should test their soils before deciding what and how much fertilizer they should apply. [ABSTRACT FROM AUTHOR]

Plain Language Summary: Diseases and weeds can reduce yield of peanut (Arachis hypogaea L.) if effective control measures including fungicides and herbicides are not used. A survey of 76 farmers in North Carolina and Virginia at Cooperative Extension Service meetings was completed relative to fungicide and herbicide use in 2021. Eighty‐two percent of farmers made between three and five herbicide applications, while 68% of farmers made between four and five fungicide applications. 2,4‐DB was the herbicide applied most frequently. The fungicide chlorothalonil was applied the most often. Pydiflumetofen was applied by 61% of farmers. Results from this survey can be used to inform regulatory agencies on pesticide use patterns by defining the relative importance of individual or groups of pesticides currently used to suppress pests and prevent or mitigate associated economic losses. [ABSTRACT FROM AUTHOR]

Foliar fungicides are available to suppress Diplodia ear rot (DER), caused by Stenocarpella maydis (Berk.) Sutton and Stenocarpella macrospora (Earle) Sutton) in corn (Zea mays L.), but previous research has indicated these fungicides have limited efficacy against the disease using traditional over‐canopy application methods. In an effort to improve coverage within the canopy and potentially improve disease control of DER, experiments were conducted in 2020, 2021, and 2022 to examine the effect of ground‐driven, in‐canopy fungicide nozzle technology on DER severity and spray coverage on the ear leaf and ear of corn plants. Application methods included over‐canopy nozzles, over‐canopy + drop nozzles, and over‐canopy + 360 Undercover nozzles. Within each application method, treatments consisted of a non‐inoculated control, or were inoculated with a conidial suspension of S. maydis. The fungicides benzovindiflupyr + azoxystrobin + propiconazole and pydiflumetofen + azoxystrobin + propiconazole were applied within each application method to measure efficacy against DER. In all years, neither fungicide product nor application method reduced DER severity. No fungicide applications increased yield compared to the non‐treated control. The addition of drop nozzles or 360 Undercover nozzles to traditional over‐canopy nozzles increased spray coverage on the ear (P < 0.0001) compared to over‐canopy nozzles alone. Plain Language Summary: Experiments conducted in Kentucky from 2020 to 2022 examined the efficacy of fungicide application method and foliar fungicides in controlling Diplodia ear rot (DER) in corn. Over‐canopy nozzles, over‐canopy + drop nozzles, and over‐canopy + 360 Undercover nozzles were tested along with two different labeled fungicides. Results showed that neither fungicide nor application method significantly reduced DER severity or increased yield compared to the non‐treated control. The addition of drop nozzles or 360 Undercover nozzles did enhance spray coverage on the ear compared to over‐canopy nozzles alone. [ABSTRACT FROM AUTHOR]

Take‐all root rot (TARR) is a detrimental disease of ultradwarf bermudagrass (Cynodon dactylon × Cynodon transvaalensis; UDB) putting greens frequently diagnosed where warm‐season grasses are grown. Since this disease is largely aggregated and variable under field conditions, field research is difficult and often yields inconsistent results. Multiple pathogens have only recently been associated with this disease, so practical management solutions such as fungicide efficacy and fungicide application timing have not been thoroughly investigated. Therefore, the objectives of this research were to determine the influence of fungicide selection and fungicide application timing on take‐all root rot management under field and greenhouse conditions. In general, fungicides from the quinone outside inhibitor and/or demethylation inhibitor chemical classes provided the greatest reduction in take‐all root rot severity. Fungicide applications that were made when soil temperatures were between 77–86°F provided the greatest disease suppression. The in vivo greenhouse method developed in this research proved to be an efficient and consistent method to evaluate management practices such as fungicide efficacy on take‐all root rot. This research improves our understanding of fungicide efficacy and fungicide application timing for take‐all root rot management on ultradwarf bermudagrass. Core Ideas: Fungicides from the QoI and/or DMI chemical class provided the greatest reduction in take‐all root rot severity.Apply fungicides when the 5‐day average soil temperature at a 2‐inch depth is approximately 80°F for TARR management.The in vivo screening method developed here is an efficient way to evaluate TARR management parameters. [ABSTRACT FROM AUTHOR]

BACKGROUND: Garlic leaf spot (GLS) caused by Alternaria alternata is one of the main diseases in the garlic production areas, and its management heavily relies on dicarboximide fungicides. However, the efficacy of dicarboximides against the GLS disease has decreased year on year. RESULTS: In the present study, 10 of 148 A. alternata strains separated from Jiangsu Province were moderately resistant (MR) to a dicarboximide fungicide procymidone (ProMR). Positive cross‐resistance was observed between Pro and iprodione (Ipro) or fludioxonil (Fld), but not between Pro and fluazinam or azoxystrobin. Mutations at AaOS1, but not Aafhk1, were confirmed to confer the Pro resistance by constructing replacement mutants, whereas mutations at both AaOS1 and Aafhk1 decreased the gene expression level of AapksI, as well as the ability to produce mycotoxin AOH (polyketide‐derived alternariol) and virulence. Additionally, more genes (AaOS1 and Aafhk1) harboring the mutations experienced a larger biological fitness penalty. CONCLUSION: To our knowledge, this is the first report on Pro resistance selected in garlic fields, and mutations at AaOS1 of A. alternata causing a decreased ability to produce the mycotoxin AOH. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Overall, there is a major wish that European farmers implement integrated pest management (IPM), particularly to reduce dependence on pesticides. In the European Rustwatch project, partners conducted nineteen trials across nine different countries during 2020 and 2021 to investigate different IPM strategies, focusing on controlling rust diseases in winter wheat. The trials included the use of varieties with contrasting levels of resistance, variety mixtures, reduced fungicide rates, thresholds, and Decision Support Systems (DSSs), and testing alternative products to fungicides. Sixteen trials developed yellow rust (Puccinia striiformis f. sp. tritici) infections, and six trials developed brown rust (Puccinia triticina) infections. Resistant varieties proved highly effective in keeping down yellow rust infection, and variety mixtures also effectively reduced infection levels and stabilized yields. Rust was fully controlled using 25% of standard fungicide rates, even under high disease pressure. Using DSSs provided sufficient control of rust diseases and resulted in competitive net economic returns due to fewer fungicide applications. The alternative products tested included two biological control agents and four alternative chemistries, which all gave inferior and insufficient control against rust compared with chemical fungicides. The trial work demonstrated that there are good and reliable options for including IPM into disease control in wheat. [ABSTRACT FROM AUTHOR]

Pesticides are indispensable in agricultural development, but their effectiveness is often hindered by natural factors like rain‐off and degradation. Herein, tebuconazole nanopesticides (TEB NPs) with leaf‐adhesive, water‐stable, and small‐sized were successfully prepared by flash nanoprecipitation using the biodegradable polyethylene glycol‐b‐poly (lactic‐co‐glycolic acid) as a carrier. The nanopesticides could be efficiently deposited on plant leaves with uniform distribution and exhibited a 14° lower contact angle with foliage compared to commercial suspensions. The encapsulation of TEB within the polymer enhanced its resistance to photolysis, resulting in a 5‐h extension of its half‐life. TEB NPs were readily taken up by fungi and plants, thereby enhancing fungicidal efficacy. This nanopesticide could achieve the desired antifungal effect with reduced dosage and improved pesticide utilization. Therefore, the work is expected to provide a simple nanoplatform for improving pesticide utilization and promoting green agriculture. [ABSTRACT FROM AUTHOR]

Bee-fungus associations are common, and while most studies focus on entomopathogens, emerging evidence suggests that bees associate with a variety of symbiotic fungi that can influence bee behavior and health. Here, we review nonpathogenic fungal taxa associated with different bee species and bee-related habitats. We synthesize results of studies examining fungal effects on bee behavior, development, survival, and fitness. We find that fungal communities differ across habitats, with some groups restricted mostly to flowers (Metschnikowia), while others are present almost exclusively in stored provisions (Zygosaccharomyces). Starmerella yeasts are found in multiple habitats in association with many bee species. Bee species differ widely in the abundance and identity of fungi hosted. Functional studies suggest that yeasts affect bee foraging, development, and pathogen interactions, though few bee and fungal taxa have been examined in this context. Rarely, fungi are obligately beneficial symbionts of bees, whereas most are facultative bee associates with unknown or ecologically contextual effects. Fungicides can reduce fungal abundance and alter fungal communities associated with bees, potentially disrupting bee-fungi associations. We recommend that future study focus on fungi associated with non-honeybee species and examine multiple bee life stages to document fungal composition, abundance, and mechanistic effects on bees.

This paper assesses the impact of viticulture on water resource quality in the Mendoza wine region using the grey water footprint (GWF) approach to estimate the amount of water required to dilute pesticides commonly used in local vineyards. Our analysis indicates that to progress towards sustainable water management in viticulture, limiting or replacing pesticides with high GWF values is essential. We provide detailed results for 24 fungicides, 7 insecticides, and 7 herbicides, assessed at both microregion and district levels, offering insights into pesticide impacts across both detailed and broader spatial scales. At the microregion level, the herbicide Fluroxypyr-meptyl was found to have the highest GWF (1.10 m³ kg-1), followed by the fungicide Fosetyl-aluminium (0.59 m³ kg-1) and the insecticide Imidacloprid (0.41 m³ kg-1). Our findings also show that pesticide impacts vary at the district level, highlighting the need for localised management strategies. Additionally, the significant variability in GWFs at the local level underscores the necessity for region-specific water quality standards to more accurately assess and manage the environmental impact of pesticide use. This study provides a framework for similar assessments in other viticultural regions, aiding in the development of more informed pesticide management to enhance water resource sustainability.

Taphrina deformans is a plant-pathogenic fungus and a responsible agent for causing peach leaf curl disease. Taphrina deformans affects peach fruit production and contributes to global economic losses. Commercial fungicides may provide temporary relief; however, their overuse resulted in adverse environmental consequences as well as led to drug-resistant strains of T. deformans. Therefore, the discovery of novel drug targets for the future synthesis of antifungal drugs against Taphrina deformans is needed. Here we studied Taphrina deformans by computational proteomics approaches. The whole genome and proteome of T. deformans were subjected to subtractive proteomics, high-throughput virtual screening, and molecular dynamic simulations. We employed subtractive proteomics analysis of 4,659 proteins extracted from UniProtKB database; after filtering out homologous and non-essential proteins, we identified 189 essential ones, including nine that participated in the crucial metabolic pathways of the pathogen. These proteins were categorized as nuclear (n = 116), cytoplasmic (n = 37), and membrane (n = 36). Of those essential proteins, glutamate–cysteine ligase (GCL) emerged as one promising target due to its essential function for glutathione biosynthesis process which facilitates T. deformans survival and pathogenicity. To validate GCL as an antifungal target, virtual screening and molecular docking studies with various commercial fungicides were carried out to better characterize GCL as a drug target. The data showed strong binding affinities for polyoxin D, fluoxastrobin, trifloxystrobin, and azoxystrobin within the active site of GCL. Polyoxin D showed a strong affinity when the measured docking score was at -7.34 kcal/mol, while molecular dynamics simulations confirmed stable interactions (three hydrogen bonds, two hydrophobic bonds, and one salt bridge interaction), supporting our findings that GCL represents an excellent target for antifungal drug development efforts. The results showed that GCL, as an innovative target for future fungicide designs to combat T. deformans infections, provides an avenue toward creating more effective peach leaf curl disease treatments while mitigating environmental harm caused by its current use.

Background: Few studies of the relationship between residential proximity to agricultural pesticide applications and pesticide levels in the home have incorporated crop location or wind direction. We evaluated the relationship between agricultural pesticide applications using the California Pesticide Use Reporting (CPUR) database and pesticide concentrations in carpet dust accounting for land use and wind direction. Methods: We measured concentrations (ng/g) of seven herbicides and two fungicides in carpet dust samples from 578 California homes (2001–2007). We created three metrics by computing the density (kg/km2) of use of each pesticide reported in CPUR within 0.5-, 1-, 2-, and 4-km buffers around homes 180- and 365-days before sampling (CPUR metric). We apportioned applications to the crop area within the buffers (CROP-A metric) and weighted CPUR applications by the proportion of days that the home was within ±45° of the downwind direction (W-CPUR metric). We modeled natural-log concentrations (Tobit regression) and dust detections (logistic regression) adjusting for season/year, occupation, and home/garden use. Results: Detections were >90 % for glyphosate, 2,4-D, and simazine. Detection rates and dust concentrations increased with increasing CPUR densities for all herbicides and one fungicide. Compared to homes without applications within 4 km, the highest tertile of 365-day glyphosate use was associated with ∼100 % higher concentrations (CPURT3>9.2kg/km2 %change = 110, 95 %CI = 55, 183; CROP-AT3>13.4kg/km2 %change = 144, 95 %CI = 81, 229; and W-CPURT3>2.1kg/km2 %change = 102, 95 %CI = 50, 171). The highest density tertiles of 2,4-D, simazine, and trifluralin were associated with 2- to 6-times higher concentrations, respectively; that was similar across metrics. Across all metrics, agricultural use of dacthal, dicamba, and iprodione were associated with 5- to 10-times higher odds of dust detections. Associations were unclear for 2-methyl-4-chlorophenoxyacetic acid and null for chlorothalonil. Conclusions: Agricultural herbicide and fungicide use was an important determinant of indoor contamination within 4 km of homes. Accounting for crops and wind direction did not substantially change these relationships.

Anthropogenic chemical pollutants, such as fungicides, pose significant threats to natural ecosystems. Although the direct impacts of numerous chemicals are well-documented in simple environmental contexts, their indirect impacts are poorly understood. This study used two individual level laboratory experiments to assess direct and indirect effects of fungicides on the isopod Asellus aquaticus, a keystone detritivore in freshwater systems. First, a range-finding assay on three widely used fungicides (Fluazinam, Tebuconazole, Urea) showed that Tebuconazole had the strongest concentration-dependent negative effects on A. aquaticus growth and food consumption. Second, a factorial experiment using Tebuconazole assessed its direct and diet-mediated effects and showed that Tebuconazole reduced growth, feeding, and pigmentation through both pathways. The results indicate that assessing only direct impacts of toxic chemicals could overlook critical interactions that are relevant in natural systems, such as those associated with diet. Our study highlights the importance of considering both direct and indirect effects in environmental toxicology to better understand the full impacts of chemical pollutants in nature.

The cultivation of oil palms is of great importance in the global agricultural industry due to its role as a primary source of vegetable oil with a wide range of applications. However, the sustainability of this industry is threatened by the presence of pathogenic fungi, particularly Ganoderma spp., which cause detrimental oil palm disease known as basal stem rot (BSR). This unfavorable condition eventually leads to significant productivity losses in the harvest, with reported yield reductions of 50–80 % in severely affected plantations. Azole-based fungicides offer potential solutions to control BSR, but their efficacy is hampered by limited solubility, penetration, distribution, and bioavailability. Recent advances in nanotechnology have paved the way for the development of nanosized delivery systems. These systems enable effective fungicide delivery to target pathogens and enhance the bioavailability of azole fungicides while minimising environmental and human health risks. In field trials, the application of azole-based nanofungicides resulted in up to 75 % reduction in disease incidence compared to conventional fungicide treatments. These innovations offer opportunities for the development of sustainable agricultural practices. This review highlights the importance of oil palm cultivation concerning the ongoing challenges posed by pathogenic fungi and examines the potential of azole-based fungicides for disease control. It also reviews recent advances in nanotechnology for fungicide delivery, explores the mechanisms behind these nanodelivery systems, and emphasises the opportunities and challenges associated with azole-based nanofungicides. Hence, this review provides valuable insights for future nanofungicide development in effective oil palm disease control.

Apple leaf blotch, a disease that is caused by the fungus Diplocarpon coronariae (also known as Marssonina coronaria) is a serious apple tree pathogen in areas with optimal conditions for its development. The fungus mainly attacks the leaves of apple trees, resulting in defoliation, which indirectly affects fruit size and color, yield and development of the apple tree shoots. In this experiment, the effectiveness of selected fungicides was tested (Sercadis – active ingredient fluxapyroxad, Belanty – active ingredient mefentrifluconazole, Topas 100 EC – active ingredient penconazole, Delan 700 WDG – active ingredient dithianon, Bellis – active ingredient boscalid and pyraclostrobin, Geoxe 50 WG – active ingredient fludioxonil, Kumulus WG – active ingredient sulphur, Cyflamid 50 EW – active ingredient cyflufenamid) for inhibiting the growth of D. coronariae using the pathogen colony cultivation method on PDA medium with addition of the fungicide. The best results were achieved by fungicides Topas 100 EC, Bellis, Sercadis and Belanty with the effectiveness of 98–100 %. Lower growth reduction was shown by Delan 700 WDG with an efficiency of 68 %. Kumulus WG, Geoxe 50 WG and Cyflamid 50 EW limited the growth of the pathogen with a reduced efficiency of 37, 22 and 15 %, respectively. There was no effect of these fungicides on growth reduction of some fungal strains.

Maize is the third most vital global cereal, playing a key role in the world economy and plant genetics research. Despite its leadership in production, maize faces a severe threat from banded leaf and sheath blight, necessitating the urgent development of eco-friendly management strategies. This study aimed to understand the resistance mechanisms against banded leaf and sheath blight (BLSB) in maize hybrid “Vivek QPM-9”. Seven fungicides at recommended doses (1000 and 500 ppm) and two plant defense inducers, salicylic acid (SA) and jasmonic acid (JA) at concentrations of 50 and 100 ppm, were applied. Fungicides, notably Azoxystrobin and Trifloxystrobin + Tebuconazole, demonstrated superior efficacy against BLSB, while Pencycuron showed limited effectiveness. Field-sprayed Azoxystrobin exhibited the lowest BLSB infection, correlating with heightened antioxidant enzyme activity (SOD, CAT, POX, β-1,3-glucanase, PPO, PAL), similar to the Validamycin-treated plants. The expression of defense-related genes after seed priming with SA and JA was assessed via qRT-PCR. Lower SA concentrations down-regulated SOD, PPO, and APX genes but up-regulated CAT and β-1,3-glucanase genes. JA at lower doses up-regulated CAT and APX genes, while higher doses up-regulated PPO and β-1,3-glucanase genes; SOD gene expression was suppressed at both JA doses. This investigation elucidates the effectiveness of certain fungicides and plant defense inducers in mitigating BLSB in maize hybrids and sheds light on the intricate gene expression mechanisms governing defense responses against this pathogen.

Background: For centuries, there has been a growing fascination with the application of herbs for fungicidal purposes worldwide. This surge in interest could be attributed to the cost-effectiveness and lower toxicities of these herbal remedies compared to the counterpart antifungal drugs. There is substantial scope to explore the curative effects of various plant components, including roots, stems, leaves and fruits of Calotropis procera, renowned for its pharmacological activities, encompassing anticancer, antimicrobial, and antioxidant. Objectives: In the present study, the antimycotic potential of the leaf and root extracts of C. procera on the growth of Aspergillus niger and Rhizopus stolonifer was investigated. Methods: The antimycotic potential of C. procera was established via the agar well diffusion method after initial preliminary phytochemical screening of the extracts. Results: Alkaloids, flavonoids, saponins, steroids, tannins and terpenoids were present, while glycosides and phenolics were tested negative. The leaf extract inhibited the tested organisms in a concentration-dependent manner where at 100% concentration, both A. niger and R. stolonifer showed high mycelia inhibition of 81.1% and 79.4%, respectively. Also, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of 31.0% and 48.0% were recorded for A. niger, as well as 28.3% and 45.7% were recorded for R. stolonifer. The aqueous root extract exhibited mycelia inhibition of 74.4% and 77.8% against A. niger and R. stolonifer at 100% concentration with respectively MIC and MFC values of 32.7% and 50.3% for A. niger and 30.3% and 52.0% for R. stolonifer. Conclusion: The study provides promising evidence of potential medicinal properties associated with C. procera, supporting its potential therapeutic applications in traditional medicine.

In this case study, the current situation faced by coffee growers attempting to control coffee leaf rust (Hemileia vastatrix) in Hawaii is reported. CLR is considered the most devastating disease affecting coffee crops worldwide and was detected in Hawaii in 2020. Three small coffee farms from the South Kona district of Hawaii Island were selected. The goals of this case study were to: (1) assist coffee growers in the early detection of CLR incidence, and consequently support farmers with recommendations for control, (2) record agronomic information and management practices, and (3) estimate the cost to control CLR during 2021 and 2022 seasons. Low CLR incidence (