151. Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint
- Author
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Holger Brüggemann, Stefan Gattenlöhner, Frank Gohlke, Frank Sommer, Alexander Brobeil, and Robert Hudek
- Subjects
Male ,Pathology ,Cross-Sectional Design ,Shoulder surgery ,SURGERY ,medicine.medical_treatment ,shoulder surgery ,Osteoarthritis ,CULTURE ,0302 clinical medicine ,PROPIONIBACTERIUM-ACNES ,Orthopedics and Sports Medicine ,MODIC CHANGES ,Skin ,030222 orthopedics ,biology ,Shoulder Joint ,Microbiota ,PERIPROSTHETIC INFECTIONS ,General Medicine ,medicine.anatomical_structure ,Immunohistochemistry ,Female ,Antibody ,Intracellular ,musculoskeletal diseases ,C acnes ,Shoulder ,medicine.medical_specialty ,Stromal cell ,DIAGNOSIS ,03 medical and health sciences ,Level III ,INFLAMMATION ,Epidemiology Study ,medicine ,Humans ,Propionibacterium acnes ,Microbiome ,ARTHROPLASTY ,Gram-Positive Bacterial Infections ,business.industry ,030229 sport sciences ,PROSTATE TISSUE ,IN-SITU HYBRIDIZATION ,medicine.disease ,intracellular ,infection ,biology.protein ,commensal ,Surgery ,Shoulder joint ,Cutibacterium acnes ,business - Abstract
Background: Cutibacterium acnes (C acnes) is a mysterious member of the shoulder microbiome and is associated with chronic postoperative complications and low-grade infections. Nevertheless, it is unclear whether it represents a contaminant or whether it accounts for true infections. Because it can persist intracellularly in macrophages at several body sites, it might in fact be an intra-articular commensal of the shoulder joint.Methods: In 23 consecutive, otherwise healthy patients (17 male, 6 female; 58 years) who had no previous injections, multiple specimens were taken from the intra-articular tissue during first-time arthroscopic and open shoulder surgery. The samples were investigated by cultivation, genetic phylotyping, and immunohistochemistry using C acnes-specific antibodies and confocal laser scanning microscopy.Results: In 10 patients (43.5%), cultures were C acnes-positive. Phylotype IA1 dominated the subcutaneous samples (71%), whereas type II dominated the deep tissue samples (57%). Sixteen of 23 patients (69.6%) were C acnes-positive by immunohistochemistry; in total, 25 of 40 samples were positive (62.5%). Overall, 56.3% of glenohumeral immunohistochemical samples, 62.5% of subacromial samples, and 75% of acromioclavicular (AC) joint samples were positive. In 62.5% of the tested patients, C acnes was detected immunohistochemically to reside intracellularly within stromal cells and macrophages.Discussion: These data indicate that C acnes is a commensal of the human shoulder joint, where it persists within macrophages and stromal cells. Compared with culture-based methods, immunohistochemical staining can increase C acnes detection. Phylotype II seems to be most prevalent in the deep shoulder tissue. The high detection rate of C acnes in osteoarthritic AC joints might link its intra-articular presence to the initiation of osteoarthritis. (C) 2020 Journal of Shoulder and Elbow Surgery Board of Trustees. All rights reserved.
- Published
- 2021
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