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The objective of the current study was to assess the effects of pre-slaughter operations in llamas on physiological (concentrations of some blood constituents) and carcass quality (pH and bruises) indicators under commercial conditions. Thirty llamas raised on pasture, 18-24 months old and average live weight of 54.2±6.4 kg were transported on a single 3 h journey in one batch to the slaughterhouse. Blood samples were taken on farm one hour before loading, after unloading, after lairage (18 h) and during exsanguination to measure the concentrations of various stress-related variables. Mean values for the blood variables showed a significant rise (P < 0.05) in the concentration of cortisol immediately after transport (16.9 ng/mL), as well as CK activity after lairage (528.4 UI/L); β-hydroxybutyrate concentration showed similar mean values for the different sampling times. A total of 19 bruises were found on 9 of the carcasses. The backs (loin) of carcasses had more bruises (57.89%), followed by thorax (21.05%). Irregularly shaped bruises were the most frequent (81.25%), followed by circular bruises (18.75%). It was concluded that pre-slaughter handling of llamas under these commercial conditions produced physiological changes similar to those in other species, which fall within acceptable limits for their welfare; however stress could be reduced and adverse effects like bruises could be minimized by designing proper facilities, by following OIE recommendations on the welfare of animals during stunning and by training of personnel handling llamas all along the Bolivian meat chain. El objetivo de este trabajo fue evaluar el efecto de las operaciones presacrificio sobre indicadores fisiológicos de estrés (constituyentes sanguíneos) y de calidad de la canal (pH y contusiones) en llamas bajo condiciones comerciales. Treinta llamas criadas a pastoreo, de 18-24 meses de edad y peso vivo 54,2±6,4 kg fueron transportadas durante 3 h en un vehículo a matadero. Se tomaron muestras de sangre a las llamas en predio una hora antes de cargar, después de descargar, después del reposo en matadero (18 h) y durante la exsanguinación. Se encontró un aumento significativo (P < 0,05) de cortisol después del transporte (16,9 ng/mL), como también de actividad de CK después del reposo (528,4 UI/L); la concentración de β-hidroxibutirato se mantuvo en los muestreos. Se registraron 19 contusiones en 9 de las 30 canales de llamas. Las lesiones superficiales, pequeñas y de color rojo brillante, fueron las más frecuentes. La región del lomo presentó más contusiones (57,89%), seguida del tórax (21,05%). Las lesiones de forma irregular fueron más frecuentes (81,25%), seguidas de circulares (18,75%). Se concluyó que las operaciones presacrificio de llamas bajo las condiciones comerciales del estudio produjeron cambios fisiológicos similares a los observados en otras especies, que caen dentro de límites aceptables para su bienestar; sin embargo, el estrés y los efectos adversos en las canales, como las contusiones, podrían minimizarse mediante diseño de estructuras apropiadas para el manejo, aplicación de las recomendaciones de OIE para el bienestar durante el sacrificio y capacitación del personal que maneja las llamas a lo largo de la cadena cárnica en Bolivia.

Conselho Nacional de Desenvolvimento Científico e Tecnológico Derivatives of propionic acid NSAIDs are irreversible inhibitors of cyclooxygenase enzyme widely used. The aim of this study was to evaluate, through different experimental models, biological effects of derivatives of propionic acid (fenoprofen, naproxen, ibuprofen and ketoprofen) in cellular and molecular level. The labeling of blood constituents with technetium-99m (99mTc) and morphological analysis of erythrocytes of blood of rats, as well as growth, survival of cultures of Escherichia coli (E. coli) and the assessment of bacterial plasmid electrophoretic profiles were models used for experimental evaluation of possible biological effects of antiinflammatory drugs. The results show that, in general, anti-inflammatory drugs evaluated were not able to alter the labeling of blood constituents with 99mTc, the morphology of red blood cells from blood of rats, as well as the growth of cultures of E. coli and the electrophoretic profile of plasmid DNA. However, naproxen appears to cytotoxic effect on bacterial cultures, plasmids and genotoxic effects in reducing the action of stannous chloride in cultures of E. coli. The use of experimental fast performance and low cost was important for assessment of biological effects, contributing to a better understanding of the properties of propionic acid derivatives studied. anti-inflammatory, blood constituents, technetium-99m, stannous chloride, Escherichia coli; DNA Os derivados do acido propiônico são antiinflamatórios não esteroidais inibidores irreversíveis da enzima cicloxigenase amplamente utilizados. O objetivo deste trabalho foi avaliar, através de diferentes modelos experimentais, efeitos biológicos de derivados do ácido propiônico (fenoprofeno, naproxeno, ibuprofeno e cetoprofeno) em nível celular e molecular. A marcação de constituintes sanguíneos com tecnécio 99m (99mTc) e a análise morfológica de hemácias de sangue de ratos Wistar, bem como, o crescimento, sobrevivência de culturas de Escherichia coli (E. coli) e a avaliação do perfil eletroforético plasmídios bacterianos, foram modelos experimentais utilizados para avaliação de possíveis efeitos biológicos dos antiinflamatórios. Os resultados obtidos demonstram que, de modo geral, os antiinflamatórios avaliados não foram capazes de alterar a marcação de constituintes sanguíneos com 99mTc, a morfologia de hemácias de sangue de ratos Wistar, assim como, o crescimento de culturas de E. coli e o perfil eletroforético de plasmídios. Entretanto, o naproxeno parece apresentar efeito citotóxico em culturas bacterianas, efeito genotóxico em plasmídios e diminuição da ação do cloreto estanoso em culturas de E. coli. A utilização de modelos experimentais de rápida realização e baixo custo se mostrou importante para avaliação de efeitos biológicos, contribuindo para uma melhor compreensão das propriedades dos derivados do ácido propiônico estudados. Esse trabalho teve caráter multidisciplinar e na vigência dos auxílios concedidos pela CAPES, FAPERJ e CNPq

Made available in DSpace on 2014-06-11T19:23:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-06-23Bitstream added on 2014-06-13T19:09:58Z : No. of bitstreams: 1 silva_prl_me_jabo.pdf: 5927581 bytes, checksum: 05e88466357134b71042346d04f0c5aa (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) O presente estudo foi constituído por três experimentos: Experimento 1: Foram analisadas as variáveis hematológicas de fêmeas bovinas da raça holandesas mensalmente durante toda a gestação e o periparto. As fêmeas foram distribuídas em 5 grupos: novilhas gestantes (G1), vacas de segunda gestação (G2), vacas de segunda gestação, repetidoras de estro(G3), vacas de terceira gestação (G4) e vacas de terceira gestação, repetidoras de estro(G5). As atividades das enzimas AST e ALP, foram maiores em G1. Houve diferença significativa entre vacas repetidoras e não repetidoras de estro somente nas concentrações séricas de colesterol no início da gestação. No segundo mês de gestação as concentrações de cálcio total e ionizado foram alteradas significativamente em relação ao primeiro mês, em todos os grupos. No terço final da gestação, dia do parto e no pós-parto houve alteração nas concentrações de cálcio, fósforo, ferro, cloretos, creatinina, bilirrubina direta e total, colesterol, triglicerídeos, proteína total, albumina e imunoglobulinas, no número de hemácias e leucócitos, volume globular, nos teores plasmáticos de glicose e hemoglobina e na atividade da enzima AST em todos grupos de animais. As atividades de LDH, ALP e GGT e as concentrações de sódio, potássio e uréia não foram significativamente alteradas durante o experimento. Experimento 2: Os constituintes sanguíneos das vacas do experimento 1 (G1) foram comparados aqueles de vacas acometidas por abortamento G (2) entre 25 (25 DPc) e 45 dias após inseminação artificial ou transferência de embrião (45 DPc). O teor de cálcio ionizado foi significativamente maior no grupo 1 45 dias após a inseminação artificial ou transferência de embrião. Possivelmente esses abortos tem origem metabólica/nutricional... The present study was constituted by 3 experiments: Experiment 1: Were analysed the blood constituents of Holstein bovine females monthly during the whole gestation and the peripartum. The females were sorted in five groups: pregnancy heifers (G1), second pregnancy cows (G2) repeat-breeders second pregnancy cows (G3), third pregnancy cows (G4) and repeat-breeders third pregnancy cows. The seric activity of AST and ALP enzymes was higher in G1. Only the cholesterol level. The diference was significative between repeat-breeders and non repeat-breeders cows only in the cholesterol seric concentration in the early gestation. In the second month of pregnancy the seric concentration of total calcium and ionized calcium was significant modified compared with the first month of gestation in all groups. In the late gestation, at the partum and in post-partum period the seric concentration of calcium, phosphorus, iron, chlorides, creatinine, direct and total bilirrubins, cholesterol, triglycerides, total protein, albumin, and imunoglobulins, number of eritrocytes and leukocytes, plasmatic concentration of glucose and hemoglobin and AST activity in all of the animal groups. The sodium, potassium and urea concentrations were not significantly modified during the experiment. Experiment 2: The blood constituents of the cows of experiment 1 (G1) was compared with cows that aborted between 25 and 45 days post-breed. Possibly this abortion has metabolic/nutricional cause, because was not found alterations in the acute-phase protein concentrations neither in the leukogram. Experiment 3: The passive immunity transfer to the heifers which were daughters of the females from the experiment 1 was evaluated by measuring seric GGT , total protein and IGg . The heifers got colostrum from their own mothers and there was no significant difference between the groups. The TIP found in all the groups was considered good.

The aim of this study was to evaluate the effect of in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. Animals were treated with cinnamon extract at different doses and for different periods of time. As controls, animals treated with 0.9% NaCl. Labeling of blood constituents with 99mTc was performed. Plasma, blood cells and insoluble fractions were isolated. Radioactivity in each fraction was counted and the percentage of radioactivity (%ATI) was calculated. Also, blood smears were prepared to morphological analysis of red blood cells from. Data showed that in vivo cinnamon extract did not significantly (p>0.05) modify the %ATI of blood constituents and morphology of red blood cells. The results suggest that in vivo aqueous cinnamon could not affect the membrane structures involved in transport of ions or the oxidation state of stannous and pertechnetate ions.O objetivo deste estudo foi avaliar efeitos do tratamento in vivo com um extrato aquoso de canela na marcação de constituintes sangüíneos com 99mTc e na morfologia de hemácias de ratos Wistar. Os animais foram tratados com diferentes doses ou por diferentes tempos com extrato de canela Como controles, animais tratados com NaCl 0,9%. A marcação de constituintes sangüíneos com 99mTc foi realizada, plasma, células sangüíneas e frações insolúveis foram isoladas. A radioatividade em cada fração foi contada e a porcentagem de radioatividade (%ATI) foi calculada. Distensões sangüíneas foram preparadas para análise morfológica de hemácias. Os dados mostraram que o tratamento in vivo com extrato de canela não modificaria significativamente (p>0,05) a %ATI nos constituintes sangüíneos e a morfologia de hemácias. Os resultados sugerem que o extrato aquoso de canela não afetaria in vivo as estruturas da membrana envolvidas no transporte de íons ou o estado de oxidação dos íons estanoso e pertecnetato.

Tomato (Solanum lycopersicum) is the second most produced and consumed vegetable in the world. It has been indicated in the prevention and treatment of cancer, asthma and atherosclerosis. Blood constituents labeled with radionuclides have been used in procedures in nuclear medicine. Data have shown that food and drugs can alter the labeling of blood constituents with technetium-99m (99mTc). This study evaluated the influence of a tomato extract on this radiolabeling procedure. Heparinized blood (Wistar rats) was incubated in vitro with different concentrations of a tomato extract and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated following soluble (SF-P/SF-BC) and insoluble (IF-P/IF-BC) fractions isolation by precipitation and centrifugation. The radioactivities on blood constituents (P, BC, IF-P, SF-P, IF-BC and SF-BC) were determined and the percentage of radioactivity (%ATI) was calculated. The tomato extract used at the highest concentrations (2.00 and 4.00g/mL), reduced significantly (p < 0.05) the %ATI in IF-P, although this extract did not modify the radiolabeling on BC, neither the radioactivity fixation on IF-BC. In conclusion, our data suggest that the chemical compounds present in the aqueous tomato extract could have some properties capable of change the fixation of 99mTc on plasma proteins. O tomate (Solanum lycopersicum) é o segundo vegetal mais produzido e consumido no mundo, tendo sido indicado para prevenção e tratamento de câncer, asma e arteriosclerose. Constituintes sangüíneos marcados com radionuclídeos têm sido usados em procedimentos na medicina nuclear. Dados têm mostrado que alimentos e drogas podem alterar a marcação de constituintes sangüíneos com tecnécio-99m (99mTc). Este estudo avaliou a influência de um extrato de tomate neste procedimento de radiomarcação. Sangue heparinizado (Wistar rats) foi incubado in vitro com diferentes concentrações de um extrato de tomate e a marcação com 99mTc foi realizada. Plasma (P) e células sangüíneas (CS) foram separadas permitindo o isolamento das frações solúvel (SF-P/SF-CS) e insolúvel (IF-P/IF-CS) por precipitação e centrifugação. A radioatividade nos constituintes sangüíneos (P, CS, IF-P, SF-P, IF-CS e SF-CS) foi determinada e a porcentagem de radioatividade (%ATI), calculada. O extrato de tomate usado, nas maiores concentrações (2,00 e 4,00g/mL), reduziu significativamente (p < 0,05) a %ATI na IF-P, embora este extrato não tenha modificado a radiomarcação da CS e fixação da radioatividade na IF-CS. Em conclusão, nossos dados sugerem que os compostos químicos presentes no extrato aquoso de tomate teriam algumas propriedades capazes de alterar a fixação do 99mTc nas proteínas plasmáticas.

Radionuclides have been used in Nuclear Medicine for diagnostic and treatment. In basic research, cellular and molecular structures are labeled with technetium-99m (99mTc) and used as radiobiocomplexes. Some natural or synthetic drugs are capable to alter the labeling of blood constituents with 99mTc, as well as in the biodistribution of radiobiocomplexes. Arctium lappa (Bardana) has been used to treat inflammatory processes. The aim of this work was to evaluate the effects of an extract of Bardana on the labeling of blood constituents with 99mTc, on the morphology of red blood cells, on the perimeter/area ratio of red blood cells and on the biodistribution of radiophamaceutical sodium pertechnetate in Wistar rats. Extract of Bardana was capable to alter the labeling of cellular compartment with 99mTc. Plasma and cellular proteins did not present alteration on the percentage of radioactivity (%ATI). Extract of Bardana was also capable to alter the morphology and the perimeter/area ratio of red blood cells. On the biodistribution of sodium pertechnetate in animals treated with the extract of Bardana, it was observed a small and significant uptake in liver, tooth and tongue, and a high and a significant uptake in stomach, lung and testis (p

This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi extract altered significantly (pEste estudo avaliou efeitos de um extrato de Ganoderma lucidum (reishi) na marcação de constituintes sangüíneos com tecnécio-99m (99mTc) e na sobrevivência de culturas de Escherichia coli tratadas com cloreto estanoso. Amostras de sangue de ratos Wistar foram tratadas com extrato de reishi, o procedimento de radiomarcação foi realizado, plasma (P), células sangüíneas (CS) e frações insolúvel (FI) e solúvel (FS) de P e CS foram separadas e a radioatividade foi contada para determinação das porcentagens de radioatividade (%ATI). Culturas de Escherichia coli AB1157 foram tratadas com cloreto estanoso na presença e ausência do extrato de reishi. Amostras de sangue e culturas bacterianas tratadas com NaCl 0.9% foram usadas como controles. Dados indicaram que o extrato de reishi alterou significativamente (p

Radiobiocomplexes are used to obtain images in nuclear medicine and employed in basic research. Blood constituents labeled with technetium-99m (99mTc) have also been employed as radiobiocomplexes and used also experimental model for evaluation of the biological effects of natural or synthetic drugs. The analysis of the morphology and the morphometrics parameters (perimeter/área ratio) can be used to evaluate the effects of drugs upon the structure of the membrane of red blood cells. Cinnamomum zeylanicum (cinnamon) is a spice used as herbal medicine to treat diseases. The aim of this study was to evaluate the effect of in vitro and in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. In the in vitro treatment, isolated blood sample from animals were incubated with cinnamon extract. In the in vivo treatment, blood samples were also withdrawn from animals treated with cinnamon extract. In both cases, the radiolabeling of blood constituents was done. The morphological analysis of red blood cells was also done. As control, blood or animals treated with NaCl 0.9%. The data obtained on the labeling of blood constituents with 99mTc experiments indicated that the in vitro treatment with cinnamon extract was capable to decrease signiicantly (p

The use of radionuclides has contributed for advances in Health Sciences, to research or to the diagnosis and/or treatment of diseases. These advances have been possible with the utilization of radiopharmaceuticals labeled with technetium-99m (99mTc). Stannous chloride (SnCl2) has the main reducing agent utilized to obtain radiopharmaceuticals labeled with technetium-99m. It has been reported that several natural or synthetic drugs are capable to alter the labeling of blood constituents with 99mTc, as well as the red blood cells morphology. The aim of this study was to evaluate possible alterations of Chrysobalanus icaco extract on the labeling of blood constituents with 99mTc, on the morphology of RBC of blood of Wistar rats, on the breakage of plasmid DNA and on the effects of stannous chloride on plasmid DNA. The results showed significant (P

Medicinal plants have been studied and used in the world. Lantana camara has medicinal properties and it has been used in folk medicine. The aim was to verify the effect of a lantana extract on the labeling of blood constituents with 99mTc, and to evaluate the effect of an aqueous extract of Lantana camara on the morphology of RBC withdrawn from Wistar rats. The results showed that lantana extract has decreased the fixation of radioactivity on the IF-P. This effect was not observed in the BC compartment and in IF-BC. The BC-%ATI was decreased in all concentrations tested when the BC was washed. The osmotic fragility assay and morphological analysis were carried out. In presence of the extract, the data obtained indicated that (i) an increase of the hemolysis and (ii) modifications on the morphology of RBC. These effects of the Lantana camara could be associated with some pharmacological properties of the chemical compounds of this studied extract Extratos de plantas medicinais são utilizados amplamente em todo o mundo. Entretanto, efeitos biológicos associados aos mesmos não têm sido investigados exaustivamente. Através do uso de suas folhas e flores a Lantana camara (Cambará de espinho) tem sido usado para tratamento de várias doenças na medicina popular. O presente estudo teve como objetivo verificar o efeito do extrato desta planta na marcação de constituintes sangüíneo com tecnécio 99m, bem como a morfologia de hemácias de ratos Wistar. Os resultados mostraram que o extrato de Lantana camara acarretou uma diminuição de fixação de radioatividade na fração insolúvel do plasma. Este efeito não foi observado na célula e nem na fração insolúvel da célula. O percentual de atividade teve diminuição em todas as concentrações quando as células foram lavadas, o que não foi observado com o controle. O extrato estudado aumentou a fragilidade osmótica das hemácias e modificou a morfologia dessas células. Os efeitos do extrato de Lantana camara podem estar associados a algumas propriedades farmacológicas de componentes químicos do extrato estudado. O estudo teve caráter multidisciplinar com a participação das seguintes áreas do conhecimento: Radiobiologia, Botânica, Fitoterapia e Hematologia

A study was carried out to determine the effect of dietary probiotic Lactina on production performance, some blood parameters and caecal microflora of male mule ducklings raised in 93 days under field conditions. The probiotic preparation Lactina consisted of freeze-dried pure cultures of Streptococcus thermophilus, Enterococcus faecium and 4 strains of Lactobacillus. Each gram of Lactina contained 0.1x109 CFU. Day-old birds were randomly allocated to -L (n = 2240) and +L (n = 2330) groups. The difference between treatments on these birds was the supplementation of the probiotic in the feeds (300 g/t) of +L group. Lactina significantly improved body weight gain, feed conversion ratio and liver weight of mules. The probiotic supplementation did not affect the intestine length, the weight of gizzard, heart, and blood constituents comprising, haemoglobin, total protein and cholesterol concentrations. Lactina fed ducklings had reduced total counts of bacteria, E. coli and Salmonella and elevated number of Lactobacilli in the caecal digesta. In addition, lower cost of body weight yield and lower mortality rate was found for the same treatment.

Seventy-five Angus x MARC II crossbred heifers were used to assess the effects of age at weaning on calf performance and blood constituents. Calves were weaned at 150, 210, or 270 days of age with comparisons made to their non-weaned counterparts. Changes in blood chemistry and hematology (cortisol, glucose, T3, total proteins, packed cell volume and the differential white blood cell count) were recorded on the day of weaning (day-0), and at 2, 7, 14, and 28 days post-weaning. Calves weaned at 210 days of age presented the highest plasma cortisol, glucose, and total protein concentrations during their early post-weaning period. Calves weaned at 150 days of age had significantly lower plasma T3 values. Packed cell volume and differential white blood cell counts showed no relationship to age at weaning. Calves weaned at 210 days of age presented the most rapid post-weaning growth performance, relative to the 150 or 270 days of age weaned calves. Setenta e cinco bezerras cruzadas Angus x MARC II foram usadas para determinar os efeitos da idade à desmama sobre a performance e sobre os constituintes do sangue. As bezerras foram desmamadas com 150, 210 ou 270 dias de idade e foram sempre comparadas com um grupo de não desmamadas. Alterações plasmáticas nas características hematológicas e químicas (cortisol, glicose, T3, proteínas totais, contagem diferencial de células brancas) foram determinadas no dia da desmama (dia-0) e nos dias 2, 7, 14 e 28 após a desmama. As bezerras desmamadas aos 210 dias de idade apresentaram as maiores concentrações de cortisol, glicose e proteínas totais no início do período pós-desmama. Bezerras desmamadas aos 150 dias de idade apresentaram valores de T3 significativamente menores. As características hematológicas (PCV e contagem diferencial de células brancas) não apresentaram relação direta com a desmama. As bezerras desmamadas aos 210 dias de idade apresentaram resposta mais rápida de crescimento no período pós-desmama, comparadas com as desmamadas aos 150 e 270 dias de idade.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:18:30Z No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Made available in DSpace on 2014-02-26T17:18:30Z (GMT). No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Previous issue date: 1998-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:25:46Z No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Made available in DSpace on 2014-05-20T15:25:46Z (GMT). No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Previous issue date: 1998-06-01 Blood constituents of Nelore, Hosltein and buffalo cows were determined during pregnancy, at the time of calving and at post-partum. It was verified that. 1. The pregnancy and post-partum periods had no influence in the erythrocyte values, but at calving the red blood cell counts and hemoglobin levels of Holstein and buffalo cows were lower than for Nelore cattle; 2. leukocyte counts were similar among groups; 3. total protein levels of Nelore cows were lower than Holstein and buffalo. The albumin levels were rite lowest at rite time of calving compared to late pregnancy; 4. glicose levels were lower ill buffalo cows during pregnancy as compared with Holstein cows. The glicemia of Nelore cows was lower as compared to Holstein cow's irt late pregnancy; 5. urea and creatinine levels were higher in buffalo cows than cattle. The urea and creatinine levels were greater in buffalo cows with maximum values at the post-partum and at the parturition, respectively; 6. bilirrubin levels were higher in bovine than buffalo cows; 7. aspartate aminotransferase activity was greater in buffalo cows and increased at the time of calving; 8. alkaline phosphatase activity was increased during pregnancy and decreased after the time of calving; 9. gammnglutamyltransferase activity,vas the highest for buffalo cows after calving; IO. calcium levels were the highest for Holstein cows at the post-partum and the phosphorus levels were higher in buffalo cows, which had the highest magnesium levels at the parturation. UNESP, Fac Cienicias Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil UNESP, Fac Cienicias Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:18:27Z No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Made available in DSpace on 2014-02-26T17:18:27Z (GMT). No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Previous issue date: 1998-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:25:45Z No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Made available in DSpace on 2014-05-20T15:25:45Z (GMT). No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Previous issue date: 1998-06-01 Newborn Holstein-Friesian and Nelore cattle and Murrah buffalo blood samples were collected for hemograma and serum constituents testing. Results indicated that: 1. buffalo red blood cell (RBC) counts were lower than RBC counts for cattle during the first 30 days after birth; 2. buffalo leukocytes, neutrophil and lymphocyte counts increased in older animals; 3. The highest fibrinogen levels occurred in older calves; 4. buffalo glucose levels were lower than Nelore cattle and decreased in the older calves; 5. buffalo iron, urea, and aspartate aminotransferase levels were higher than in cattle with the highest concentration at the time of birth; 6. cattle bilirubin and alanine aminotranferase levels were higher than buffalo with the highest value at the time of birth; 7. alkaline phosphatase and gammaglutamyltransferase activities were lower in the older calves; 8. buffalo magnesium levels were higher than cattle with lower concentrations at the time of birth.. UNESP, Fac Ciências Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FCAVJ, Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil UNESP, Fac Ciências Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FCAVJ, Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil