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Objectives: To summarize the clinical characteristics and investigate the efficacy of ethanol embolotherapy in the treatment of chest well arteriovenous malformation (AVM). Treatment-associated complications were also explored. Materials and Methods: Between March 2017 and August 2021, 32 consecutive patients (mean age, 23.7 years; age range, 5–54 years) who underwent ethanol embolotherapy for chest well AVMs under general anesthesia were included in this study. Embolization was performed through a direct puncture, transarterial catheterization, or a combination of the 2 procedures. The mean follow-up duration after the last treatment was 18.0 months (range, 3–42 months). The degree of devascularization on follow-up (assessed using angiography or computed tomography), and the clinical signs and symptoms of AVMs were evaluated as the therapeutic outcomes. The major and minor complications associated with the procedures were recorded. Results: A total of 103 embolization procedures (mean, 3.2; range, 2–7) comprising 101 ethanol embolization and 2 coil embolizations were performed on 32 patients with chest wall AVMs. The AVM nidus was accessed through the transarterial approach alone in 4 patients, by direct puncture in 11, and a combined procedure in 17 patients. Overall, more than 80% of the procedures were performed using the combined approach. Complete AVM devascularization was achieved in 12 (37.5%) patients. Moreover, 76% to 99% AVM was achieved in 18 patients (56.3%), and 50% to 75% in 2 patients (6.3%). Bleeding, pain, heart failure, and cosmetic deformities were the indications for treatment. For 3 patients (3/32, 9.4%) who had bleeding, the treatment stopped the hemorrhage. Complete pain relief was reported in 8 patients (8/32, 25.0%), whereas complete relief from congestive heart failure post-embolization was observed in 5 of the 6 patients with congestive heart failure (5/6, 83.3%). Complete correction of cosmesis deformities after embolization was achieved in 10 patients (10/32, 31.3%). Two patients who underwent surgery to correct persistent deformity after embolization only showed insignificant improvement. In addition, 6 (18.8%) patients developed 13 complications including blister, necrosis, hemothorax, transient hemoglobinuria, and transient pulmonary artery hypertension. Conclusions: Ethanol embolotherapy is a safe and effective procedure for chest well AVMs. Surgery is required for some patients with residual cosmesis deformity. Clinical Impact Currently, there is no standard treatment for chest well AVMs due to their rarity and high heterogeneity. The present study shows that thanol embolotherapy is a safe and clinically effective treatment procedure for the chest well AVMs. Transarterial embolization in combination with direct puncture embolization can reach the AVM nidus. Ethanol embolotherapy can achieve complete obliteration of the AVM nidus in the majority of patients. Surgery may still be needed to correct cosmetic deformity after embolization. The present study provides valuable evidence to inform clinical decision-making.

The pharmacokinetics and tissue distribution of enrofloxacin were determined in Yellow River carp (

2′,5′-oligoadenylate synthase (OAS) is a class of enzymes induced by interferons and mainly encoded by the OAS1, OAS2, and OAS3 genes, which activate the potential RNA enzymes to degrade viral mRNA, inhibit viral protein synthesis and promote apoptosis in virus-infected cells.OAS3is associated with breast cancer prognosis. However, the expression and prognosis ofOAS3and tumour-infiltrating lymphocytes in pan-cancer remain unknown. In the present study, we have systematically investigated and confirmed the role ofOAS3in tumour immune infiltration, immune escape, tumour progression, response to treatment, and prognosis of different cancer types using various bioinformatics methods. The findings suggest thatOAS3is aberrantly expressed in almost all TCGA cancer types and subtypes and is associated with tumour staging, metastasis, and prognostic deterioration in different tumours. In addition,OAS3expression is associated with the prognosis and chemotherapeutic outcomes of various cancers. In terms of immune-infiltrating levels,OAS3expression is positively associated with the infiltration of immunosuppressive cells. These findings suggest thatOAS3is correlated with prognosis and immune-infiltrating levels.

BackgroundIdiopathic pulmonary fibrosis (IPF) is one of interstitial lung diseases (ILDs) with poor prognosis. S100 calcium binding protein A12 (S100A12) has been reported as a prognostic serum biomarker in the IPF, but its correlation with IPF remains unclear in the lung tissue and bronchoalveolar lavage fluids (BALF).MethodsDatasets were collected from the Gene Expression Omnibus (GEO) database. Person correlation coefficient, Kaplan–Meier analysis, Cox regression analysis, functional enrichment analysis and so on were used. And single cell RNA-sequencing (scRNA-seq) analysis was also used to explore the role of S100A12 and related genes in the IPF.ResultsS100A12 was mainly and highly expressed in the monocytes, and its expression was downregulated in the lung of patients with IPF according to scRNA-seq and the transcriptome analysis. However, S100A12 expression was upregulated both in blood and BALF of patients with IPF. In addition, 10 genes were found to interact with S100A12 according to protein–protein interaction (PPI) network, and the first four transcription factors (TF) targeted these genes were found according to hTFtarget database. Two most significant co-expression genes of S100A12 were S100A8 and S100A9. The 3 genes were significantly negatively associated with lung function and positively associated with the St. George’s Respiratory Questionnaire (SGRQ) scores in the lung of patients with IPF. And, high expression of the 3 genes was associated with higher mortality in the BALF, and shorter transplant-free survival (TFS) and progression-free survival (PFS) time in the blood. Prognostic predictive value of S100A12 was more superior to S100A8 and S100A9 in patients with IPF, and the composited variable [S100A12 + GAP index (gender, age, and physiological index)] may be a more effective predictive index.ConclusionThese results imply that S100A12 might be an efficient disease severity and prognostic biomarker in patients with IPF.

The Genome Sequence Archive for Human (GSA-Human) is a data repository specialized for human genetic related data derived from biomedical researches, and also supports the data collection and management of National Key Research and Development Projects. GSA-Human has a data security management strategy according to the national regulations of human genetic resources. It provides two different models of data access: Open-access and Controlled-access. Open-access data are universally and freely accessible for global researchers, while Controlled-access ensures that data are accessed only by authorized users with the permission of the Data Access Committee (DAC). Till July 2021, GSA-Human has housed more than 5.27 PB of data from 750 datasets.GSA-Human是人类遗传资源数据汇交、存储、管理与共享的数据库系统,可提供人类遗传资源数据的上传、下载、浏览、检索等公共服务,并有效支撑了国家重点研发计划科技项目数据的汇交与管理工作。系统具有符合《中华人民共和国人类遗传资源管理条例》数据安全管理策略,提供公开访问和受控访问相结合的数据使用模式。公开访问数据允许用户自由下载与获取;受控访问数据采用申请-审核的模式,即需要通过数据管理委员会(Data Access Committee, DAC)的授权方可获得下载和使用权限。系统自上线以来,截至2021年7月,汇集数据总量已超5.27 PB。.

Funding Information: Financial support from the Natural Science Foundation of China under Contracts 21808163 and 21690083 is gratefully acknowledged. Publisher Copyright: © 2021 American Chemical Society. A solvent-free method was proposed to upgrade the biomass-derived compound 5-hydroxymethylfurfural (HMF). The oxidation of HMF to produce 2,5-furandicarboxylic acid (FDCA) has been examined in the presence of O2 without the addition of solvent and base. Different from the conversion of the aldehyde group on HMF as the initial oxidation step in H2O solvent, the hydroxyl group on HMF was first oxidized and FDCA was finally generated without the addition of solvent. The role of O2 is to replenish the consumption of active oxygen species on the catalyst surface. The oxidation of HMF to FDCA proceeded due to the solvent-free effect. A 83.6% FDCA selectivity at 38.8% HMF conversion was measured with a PdO/AlPO4-5 catalyst at 80 °C for 5 h and the reaction mechanism was proposed.

We have previously reported on the potential pathogenic role of neutrophils in biliary atresia (BA). Herein, we aimed to delineate the role of CD177+ neutrophils in the pathogenesis of BA.Immune cells from the livers of mice with rhesus rotavirus-induced BA were analysed. Single-cell RNA-sequencing was performed to specifically analyse Gr-1Increased levels of Gr-1Our data indicate that CD177The pilot study of N-acetylcysteine treatment in patients with BA was registered on the Chinese Clinical Trial Registry (ChiCTR2000040505).Neutrophils (a type of innate immune cell, i.e. an immune cell that doesn't target a specific antigen) are thought to play a role in the development of biliary atresia (a rare but potentially lethal condition of the bile ducts that occurs in infants). Herein, we found that neutrophils expressing a particular protein (CD177) played an important role in bile duct damage by releasing a special structure (NET) that can trap and kill pathogens but that can also cause severe tissue damage. A pilot study in patients with biliary atresia showed that inhibiting NETs could have a beneficial effect.

Background: In this study, a prognostic model based on pyroptosis-related genes was established to predict overall survival (OS) in patients with hepatocellular carcinoma(HCC).Methods: The gene expression data and clinical information of HCC patients were acquired from The Cancer Genome Atlas (TCGA). Using bioinformatics analysis, this predictive signature was constructed and validated. The performance of predictive signature was assessed by the receiver operating characteristic (ROC) curve and the area under the ROC curve (AUC). Results: A total of 3 pyroptosis-related genes (BAK1, GSDME, and NOD2) were used to construct a survival prognostic model, and experimental validation performed using an experimental cohort. The prognosis model exhibited good performance based on the AUC (AUC: 0.826 at 1 years, 0.796 at 3 years, 0.867 at 5 years). The calibration plots showed excellent calibration.Conclusion: In this study, a novel prognostic model based on three pyroptosis-related genes is constructed and used to predict the prognosis of HCC patients. The model can accurately and conveniently predict the 1- 3-and 5-year OS of HCC patients.

Background Extrahepatic portal vein obstruction (EHPVO) is the most important cause of hematemesis in children. Intrahepatic left portal vein and superior mesenteric vein anastomosis, also known as meso-Rex bypass (MRB), is becoming the gold standard treatment for EHPVO. We analyzed the value of preoperative computed tomography (CT) in determining whether MRB is feasible in children with EHPVO. Results We retrieved data on 76 children with EHPVO (50 male, 26 female; median age, 5.9 years) who underwent MRB (n = 68) or the Warren procedure (n = 8) from 2013 to 2019 and retrospectively analyzed their clinical and CT characteristics. The Rex recess was categorized into four subtypes (types 1–4) depending on its diameter in CT images. Of all 76 children, 7.9% had a history of umbilical catheterization and 1.3% had leukemia. Sixteen patients (20 lesions) had associated malformations. A total of 72.4% of Rex recesses could be measured by CT, and their mean diameter was 3.5 ± 1.8 mm (range 0.6–10.5 mm). A type 1, 2, 3, and 4 Rex recess was present in 9.2%, 53.9%, 11.8%, and 25.0% of patients, respectively. MRB could be performed in patients with types 1, 2, and 3, but those with type 4 required further evaluation. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of CT were 100%, 83.8%, 42.1%, 100%, and 85.5%, respectively. Conclusions Among the four types of Rex recesses on CT angiography, types 1–3 allow for the performance of MRB.

Background Biliary atresia (BA) is a severe cholangiopathy possibly resulting from virus-induced and immune-mediated injury of the biliary system. IFN-γ, secreted from CD4+ Th1 cells and CD8+ cytotoxic T cells, is a major mediator of liver pathology. Programmed death protein-1 (PD-1) signaling suppresses T cell function. However, how PD-1 modify T cell function in BA remains incompletely understood. Methods Frequencies of PD-1 expressing CD4+ and CD8+ T cells were analyzed in the liver and blood from BA and control subjects. Associations of PD-1+CD4+/CD8+T cell abundances with liver function indices were measured. Function of PD-1 was measured by administration of an anti-PD-1 antibody in a Rhesus Rotavirus (RRV)-induced BA model. Survival, histology, direct bilirubin, liver immune cell subsets and cytokine production were analyzed. Results PD-1 was significantly upregulated in CD4+ and CD8+ T cells in patients with BA compared with control subjects. PD-1 expression in T cells was negatively associated with IFN-γ concentration in liver (PD-1+CD4+T cells in liver vs. IFN-γ concentration, r = − 0.25, p = 0.05; PD-1+CD8+T cells in liver vs. IFN-γ concentration, r = − 0.39, p = 0.004). Blockade of PD-1 increased IFN-γ expression in CD4+ T and CD8+ T cells (RRV vs. anti-PD-1 treated RRV mice: 11.59 ± 3.43% vs. 21.26 ± 5.32% IFN-γ+ in hepatic CD4+T cells, p = 0.0003; 9.33 ± 4.03% vs. 22.55 ± 7.47% IFN-γ+ in hepatic CD8+T cells, p = 0.0001), suppressed bilirubin production (RRV vs. anti-PD-1 treated RRV mice: 285.4 ± 47.93 vs. 229.8 ± 45.86 μmol/L total bilirubin, p = 0.01) and exacerbated liver immunopathology. Conclusions PD-1 plays a protective role in infants with BA by suppressing IFN-γ production in T cells. Increasing PD-1 signaling may serve as a therapeutic strategy for BA.

Exosomes, one of three main types of extracellular vesicles, are ~30-100 nm in diameter and have a lipid bilayer membrane. They are widely distributed in almost all body fluids. Exosomes have the potential to regulate unknown cellular and molecular mechanisms in intercellular communication, organ homeostasis, and diseases. They are critical signal carriers that transfer nucleic acids, proteins, lipids, and other substances into recipient cells, participating in cellular signal transduction and material exchange. ncRNAs are non-protein-coding genes that account for over 90% of the genome and include microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs). ncRNAs are crucial for physiological and pathological activities in the liver by participating in gene transcription, posttranscriptional epigenetic regulation, and cellular processes through interacting with DNA, RNA, or proteins. Recent evidence from both clinical and preclinical studies indicates that exosome-derived noncoding RNAs (ncRNAs) are highly involved in the progression of acute and chronic liver diseases by regulating hepatic lipid metabolism, innate immunity, viral infection, fibrosis, and cancer. Therefore, exosome-derived ncRNAs have promising potential and clinical implications for the early diagnosis, targeted therapy, and prognosis of liver diseases.

Introduction: Pyogenic liver abscess (PLA) is a serious infectious disease of the liver. Pyogenic liver abscess caused by Fusobacterium nucleatum is extremely rare. Here we report the first case of liver abscess caused by F. nucleatum in China. Case Presentation: The case was a 34-year-old female patient admitted to the hospital due to high fever. The diagnosis of liver abscess was confirmed by imaging studies and liver puncture. We finally confirmed the pathogen as F. nucleatum by next-generation sequencing (NGS). After the targeted anti-infective treatment, the patient recovered and discharged. Conclusions: As a new microbial detection method, NGS can still help in clinical practice. In addition, to improve the positive rate of anaerobic bacteria culture, we should pay attention to avoid contact with air in the process of specimen collection when the pathogenic bacteria are suspected to be anaerobic bacteria.

FDG PET/CT was performed for staging in a 15-year-old adolescent girl with cholangiocarcinoma, which showed only mild activity in the tumor but more impressive FDG activity in right femoral fibrous cortical defect without any other hypermetabolic lesions elsewhere. Pathological examination of the resected cholangiocarcinoma revealed significant neuroendocrine differentiation, which lead to subsequent Ga-DOTATATE PET/CT study. Unexpectedly, the same femoral fibrous cortical defect also had increased Ga-DOTATATE activity.

The catalytic conversion of microcrystalline cellulose is examined over a niobic acid catalyst (NBO) prepared by an alkali fusion method. The catalytic activity of NBO is significantly affected by ...

Introduction and objectives: This study assessed the diagnostic protocol and the outcomes of laparoscopic aberrant hepatic duct (AHD) reconstruction in choledochal cyst (CC) surgery. Methods: From January 2010 to January 2018, 275 laparoscopic CC excisions were conducted in our hospital. Seven patients of CC with associated AHD were recorded. AHDs that drained into the cystic duct were anastomosed to the Roux-en-Y loop. Clinical data of these 7 patients were retrospectively analyzed. Results: AHD is classified into four types according to the literature. The type where AHD drains into the cystic duct is the most commonly encountered one. The incidence of this type was 2.5% (7/275) in our series. Preoperative examination included magnetic resonance cholangiopancreatography (MRCP) (5 patients) and computed tomography (CT) scan (2 patients). Suspected AHD was diagnosed preoperatively in 4 out of the 5 patients who underwent MRCP. For the 2 patients who underwent CT scan only, AHD was not detected preoperatively. AHD was verified intraoperatively for all the 4 patients who had suspected diagnosis. Among them, AHD was well preserved in 3 patients, but damaged at exploration in the remaining 1. For the remaining 3 patients without preoperative AHD diagnosis, bile leakage was detected intraoperatively. Ductoplasty of the AHD with common hepatic duct was performed in 3 patients, and AHD was incorporated into the Roux-en-Y jejunal loop separately in 4 patients. Laparoscopic surgical procedures were completed in 6 of the 7 patients in this study, while 1 patient was converted into an open procedure. Postoperative recovery was uneventful in all patients. The duration of follow-up ranged from 6 months to 4 years (median 2 years) with no complication encountered. Conclusions: Routine preoperative MRCP examination of CC is recommended to detect variations of biliary tree. The laparoscopic approach is a feasible option in the experienced hands.

Rosai-Dorfman disease is typically presented as lymphadenopathy, especially in the neck. Extranodal diseases also frequently occur but are generally concurrent with nodal disease. We report FDG PET/CT findings of solitary femoral Rosai-Dorfman disease without any lymphadenopathy in a pediatric patient.

A novel fiber-optic surface plasmon resonance sensor for detection of Cu2+ ions in an aquatic environment is proposed and experimentally demonstrated. The proposed sensor is fabricated by splicing no-core fiber between multimode fibers and then functionalizing the sensor surface with gold film and chitosan (CS)/poly(acrylic acid) (PAA) bilayers. The gold film is used to stimulate surface plasmon waves and make the sensor sensitive to changes in refractive index induced by Cu2+ ions absorption on the CS/PAA bilayers. The proposed sensor has a linear relationship between the wavelength shifts and the concentrations of Cu2+ ions from 0.2 to 50 μ M and 50 to 500 μ M, respectively. It is also observed that the sensor's performance is better at low concentrations, and its sensitivity decreases with the increasing concentration. Moreover, we find the proposed sensor can express the self-assembly process between CS and PAA clearly, which shows us a new and easy way to investigate the polyelectrolyte self-assembling.

A WO3/γ-Al2O3 catalyst prepared by an incipient-wetness impregnation method is employed in the selective conversion of enzymatic hydrolysis lignin (EHL) into various alkylphenols in supercritical e...

When V2O5 is used for the deoxygenation of guaiacol in methanol, it is reduced in situ to haggite structured V4O6(OH)4. Guaiacol prevents further reduction of the haggite phase in methanol and haggite catalyzes the partial deoxygenation of guaiacol. Haggite is a metastable redox catalyst for the deoxygenation of guaiacol, which follows the reverse Mars–van Krevelen mechanism. In addition, haggite is also a Lewis acid catalyst and catalyzes the alkylation of guaiacol with methanol as the alkylation reagent. The main products of the guaiacol deoxyalkylation are 2,6-dimethylphenol, 2-methoxy-6-methylphenol, 2,4,6-trimethylphenol, 2,3,6-trimethylphenol, 2,3,5,6-tetramethylphenol and 6-methyl-2-tert-butylphenol. Oligomerization takes place during the reaction but it is reversible. When the reaction is performed at 300 °C for 6 h, the 83.5% total selectivity for alkylphenols is achieved with a 99.0% conversion.

The conversion of guaiacol is examined at 300 °C in supercritical ethanol over a H 2 WO 4 catalyst. Guaiacol is consumed completely, meanwhile, 16.7% aromatic ethers and 80.0% alkylphenols are obtained. Interestingly, tert-butylphenols are produced mainly with a high selectivity of 71.8%, and the overall selectivity of 2,6-di-tert-butylphenol and 2,6-di-tert-butyl-4-ethylphenol is as high as 63.7%. The experimental results indicate that catechol and 2-ethoxyphenol are the intermediates. Meanwhile, the WO 3 sites play an important role in the conversion of guaiacol and the Brønsted acid sites on H 2 WO 4 enhance the conversion and favour a high selectivity of the tert-butylphenols. The recycling tests show that the carbon deposition on the catalyst surface, the dehydration and partial reduction of the catalyst itself are responsible for the decay of the H 2 WO 4 catalyst. Finally, the possible reaction pathways proposed involve the transetherification process and the alkylation process during guaiacol conversion.

A dual-function terahertz metasurface based on VO2 and graphene is proposed in this paper. It consists of a gold layer embedded with VO2 patches, a SiO2 spacer layer, a VO2 layer, graphene and a SiO2 spacer substrate. When the bottom VO2 layer is in the metallic state, the designed metasurface can achieve absorption. When the top VO2 patches are in the metallic state, the proposed metasurface can be used as a single-band absorber with terahertz absorptance of 99.7% at 0.736 THz. When the top VO2 patches are in the insulating state, the designed structure behaves as a dual-band absorber with an absorptance of 98.9% at 0.894 THz and 99.9% at 1.408 THz. In addition, the absorber is polarization insensitive and keeps good performance at large angles of incidence. When the bottom VO2 is in an insulating state, the metasurface shows electromagnetically induced transparency. The transparent window can be dynamically regulated by controlling the chemical potential of graphene. The proposed metasurface exhibits the advantages of terahertz absorption, electromagnetically induced transparency and dynamic control, which provides more options for the design of terahertz devices in the future.

Background The chronic visceral subtype of acid sphingomyelinase deficiency, commonly known as Niemann Pick disease type B (NPDB), is a relatively rare autosomal recessive genetic disorder that is caused by mutations in the SMPD1 gene. NPDB with sea-blue histiocytes (SBH) clinically mimics Budd-Chiari syndrome (BCS), as it lacks specific clinical characteristics. This makes its diagnosis difficult. Case presentation Here, we report a case of NPDB with SBH that was misdiagnosed as BCS for three years. A 20-year-old female with abdominal distension, hepatosplenomegaly, and haematological anomalies was initially diagnosed with BCS based on her imaging finding of a thin hepatic vein and rapid blood flow at the confluence of the hepatic vein and inferior vena cava. Her bone marrow cytology found sea-blue histiocytes. Liver biopsy showed foamy cytoplasm in hepatocytes surrounded by numerous Kupffer cells. Sequencing analysis of the SMPD1 gene led to the finding of two missense mutations in the heterozygous state: C.829 T > C (p.Trp277Arg) in exon 2 (novel) and c.1805G > A (p.Arg602His) in exon 6 (already described). These findings established the diagnosis of NPDB. Conclusion The patient presented with hepatosplenomegaly, haematological anomalies, and dyslipidaemia. Thus, NPDB should be considered following the exclusion of related diseases. The diagnosis of NPDB was suspected by clinical symptoms and routine laboratory tests and was confirmed by liver biopsy and gene sequencing. The novel mutation c.829 T > C in exon 2 of the SMPD1 gene has never been reported and needs to be further investigated.

Nitro polycyclic aromatic hydrocarbons (NPAHs) are important pollutants with carcinogenic effects present in PM

A 43-year-old woman with edema in both left upper limb and lower limb underwent lymphoscintigraphy to evaluate possible lymphedema. Two separate lymphoscintigraphies, one for the lower and the other for the upper limbs, were performed with interval of 3 days. Incidentally, an activity in the region of the thyroid glands was noted in the delayed images of both studies. Subsequently, Graves disease was diagnosed based on the patient's symptoms and the results of laboratory examinations.

Selective oxidation of 5-hydroxymethylfurfural (HMF) to 2, 5-diformylfuran (DFF) with molecular oxygen is realized with an α-MnO2 catalyst under mild conditions. In this work, α-MnO2 exhibited the best performance among the samples examined. Meanwhile, solvent shows a significant effect on the product selectivity and isopropanol is found good for improving the selectivity of DFF. 93.2% conversion of HMF was achieved at 140 °C for 4 h with 84.3% selectivity of DFF. Moreover, α-MnO2 catalyst keeps good reusability in recycling for five times. The reaction pathway indicated that the lattice oxygen species on α-MnO2 is involved in the selective oxidation of hydroxyl group in HMF molecule.

Background: Biliary atresia (BA) is a severe cholangiopathy resulting from virus-induced and immune-mediated injury of the biliary system. IFN-g, secreted from CD4+ Th1 cells and CD8+ cytotoxic T cells, is a major mediator of liver pathology. Programmed death 1 (PD-1) signaling suppresses T cell function. However, how PD-1 modify T cell function in BA remains incompletely understood. Methods: Frequencies of PD-1 expressing CD4+ and CD8+ T cells were analyzed in the liver and blood from BA and control subjects. Associations of PD-1+CD4+/CD8+T cell abundances with liver function indices were measured. Function of PD-1 was measured by administration of an anti-PD-1 antibody in a Rhesus Rotavirus (RRV)-induced BA model. Survival, histology, direct bilirubin, liver immune cell subsets and cytokine production were analyzed. Results: PD-1 was significantly upregulated in CD4+ and CD8+ T cells in patients with BA compared with control subjects. PD-1 expression in T cells was negatively associated with IFN-γ concentration in liver. Blockade of PD-1 increased IFN-g expression in CD4+ T and CD8+ T cells, suppressed bilirubin production and exacerbated liver immunopathology.Conclusions: PD-1 plays a protective role in infants with BA by suppressing IFN-g production in T cells. Increasing PD-1 signaling may serve as a therapeutic strategy for BA.

The barrier layer (BL) is a layer of water separating the thermocline from the density mixed layer in the upper ocean, which has the capability of reducing the negative feedback effect caused by tropical cyclone (TC) acting on the upper ocean and back on the TC itself. This study analyzed in-situ Argo floats measurements, data-assimilated HYCOM/NCODA reanalysis, and the longer-term (1961–2010) variations of Ocean Reanalysis System 4 (ORAS4) based BL in the TC main development region (MDR) to characterize the BL in the western North Pacific (WNP) for different temporal scales and to understand its role in resisting TC induced sea surface cooling. First, the result indicates that the effect of BL on TC enhancement in the MDR of WNP might be overestimated. Further analysis based on partial correlation shows that the BL plays a key role in resisting the cooling response only while BL is strong (BL thickness ≥ 5 m) and TC wind forcing is weak. Meanwhile, the distribution of BL demonstrates markedly the mesoscale characteristic. BL with thickness 0–5 m occupies the highest proportion (~67.55%), while thicker BL (BL thickness (BLT) larger than 5 m) takes up about 25–30%. Besides, there are ~3% with BL thicker than 30 m. For life length, BLT with 0–5 m is limited to 5 days, while BL with thickness more than 30 m can persist for more than 30 days. The scenario is attributed to diverse processes that result in different characteristic temporal scales of BL. Additionally, the analysis of coverage region and average BLT in the recent decade shows a serious situation: both the spatial coverage and BLT increase sharply from 2001 to 2010, which implies that TC–BL interactions might occur more frequently and more vigorously in future if the changing trend of BL remains unchanged.

A La-MnO2 catalyst exhibited excellent performance in aerobic oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA). Characterization of the samples revealed that the loose surface morphology and high oxygen species mobility facilitated the HMF oxidation reaction. H2O was verified as a good solvent for the formation of FDCA because of the facilitation of aldehyde group hydration to generate a carboxyl group on the HMF molecule. A 95.4% FDCA selectivity at 96.3% HMF conversion was achieved in H2O under mild conditions.

BackgroundTo explore the changes of chlorophyll content in needles of different leaf ages of Picea koraiensis Nakai of different specifications, the study compared the prediction accuracy changes of chlorophyll in needles of Picea koraiensis Nakai by two modeling methods: the BP neural network and partial least squares regression (PLSR) methods. The effects of different spectral pre-processing and characteristic band selection methods on the performance accuracy of the model were tested, and the optimal combination model was selected to predict forest growth status and community structure productivity through the physiological and biochemical characteristics of needles at different leaf ages. Results1) the spectral pre-processing method could avoid systematic errors and eliminate background values; 2) the accuracy of the needle chlorophyll fitting model with different leaf ages was much higher than that of mixed needle chlorophyll model, verifying that needle chlorophyll with different leaf ages could better estimate the annual growth and examine the growth status of Picea koraiensis Nakai; 3) the accuracy of the BP neural network model was significantly higher than that of the PLSR model, with its R 2 above 0.95, and the validation set’s R 2 above 0.86; and 4) the fitting accuracy of different leaf age needle chlorophyll models of the spectral pre-processing model, variable selection model, PLSR model and BP neural network: triennial needles > annual and biennial needles. ConclusionsThe BP neural network method was more accurate than the PLSR method in predicting pigment content model. In the process of model fitting, it was found that the pigment model fitted by fine classification of needles improves the accuracy of the model, which provides the basis and theoretical support for the establishment of the model by combining remote sensing technology with stoichiometry methods in the future.

It is of great significance to timely and accurately monitor the vegetation water content (VWC) for the study of plant growth status, drought assessment, and fire risk forecasting. Global Positioning System Interferometric Reflectometry (GPS-IR) is a new type of remote sensing technology. The signal-to-noise ratio recorded by the measurement receiver can be used to retrieve VWC effectively. Although studies have fully proved that the change of VWC can be effectively reflected by the normalized microwave reflectance index (NMRI), the NMRI based on a single ground-based GPS-IR cannot achieve spatial continuity. This paper makes full use of the advantages of multiple ground-based GPS-IR combined with vegetation index, weather, topography and other related elements, and a nonlinear inversion method of high spatial resolution VWC based on multiple ground-based GPS-IR is proposed. Firstly, First, various elements are acquired through Google Earth Engine, and the resolution is unified through ArcGIS; Then, the relationship model between the multiple ground-based GPS-IR and related elements is established through the least square support vector machine (LS-SVM), and the modeling effects of different model input variables are compared and analyzed, and the best modeling solution is selected; Finally, after model testing, the accurate inversion of VWC is realized. Taking a certain area of the United States as the research object, experiments show that: 1) Different fac-tors have different influences on modeling accuracy. Three vegetation indices of NDVI, GPP and LAI > topography > longitude and latitude of the station > daily average rainfall and daily average temperature. Among them, NDVI is a key element that affects modeling accuracy. 2) Using LS-SVM can effectively integrate multiple ground-based GPS-IR and related elements, and the model fitting process is relatively stable; the 16Day/500 m resolution NMRI image obtained by model inversion can better reflect regional VWC changes. 3) The model inversion error is relatively stable. The maximum error is only 0.031, and the root mean square error is only 0.040.

Enrofloxacin (ENR) has been approved for the treatment of infections in aquaculture, but it may cause tissue residue. This research aimed to develop and validate a water temperature related PBPK model, including both ENR and ciprofloxacin (CIP), in rainbow trout, and to predict further their residue concentrations and the withdrawal periods for ENR at different water temperatures. With the published concentrations data, a flow-limited PBPK model including both ENR and CIP sub-models was developed to predict ENR and CIP concentrations in tissues and plasma/serum after intravenous, oral, or immersion administration. A Monte Carlo simulation including 500 iterations was further incorporated into this model. Based on the model and Monte Carlo analysis, the withdrawal intervals were estimated for different dosage regimens and at different water temperatures, ranging from 80 to 272 degree-days. All of these values were shorter than the labeled withdrawal period (500 degree-days) in fish. This model provided a useful tool for predicting the tissue residues of ENR and CIP in rainbow trout under different dosage regimens and at different water temperatures.

Diabetes is a chronic illness with severe impacts on the patient’s health. Specifically, diabetes may lead to other complications, such as diabetic foot ulcerations. Diabetic foot ulcers progressively deteriorate without proper treatment, with infections leading to hospitalization and amputation. However, loss of sensation resulting from diabetic neuropathy and other complications may result in these foot ulcers going unnoticed. One of the preventing strategies is to use monitoring devices to detect early onset of diabetic foot ulcers. Previous studies used plantar pressure or joint mobility as indicators to design such devices. However, very few studies combined both measurements. The measuring systems involved are also difficult to operate. The aim of this paper is to present a non-invasive household tool prototype for monitoring and early detection of diabetic ulcers by combining plantar pressure measurements from TEKSCAN HUGE MAT and hip range of motion measurements from a gyroscope sensor installed in smartphones. This new prototype models the situation where a patient walks on a floormat while the phone is located within the front pants pocket at home, which requires minimal human intervention to predict the risk of diabetic ulcerations. Further testing shows that this prototype can obtain measurements with reasonable accuracy.

This study used a numerical model to simulate the floating trajectory of garbage falling into the sea from the landfill near the coast of Wanghaixiang Bay in northeast Taiwan to understand its impact on the local environment. The Regional Ocean Model System was used to simulate the probability densities of the distribution of garbage drifting trajectories under scenarios of no-wind, northeast monsoon, and typhoons. The results show that, in the no-wind scenario, garbage was mainly affected by tidal currents. It moved in the northwest–southeast direction outside the bay. In the northeast monsoon scenario, garbage was forced toward the shore due to the windage effect. In typhoon scenarios, strong winds forced the garbage to the shore, as typhoons continued to advance and the wind direction kept changing, the garbage trajectory was also changed. After typhoons moved away, the drifting trajectory of the garbage was again affected by tidal currents. When the garbage falling into the sea was located in the bay or the mouth of the bay, the garbage had a higher probability of being forced into the bay by typhoons.