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Background Both stroke and depression are multi-factorial diseases, with both genetic and environmental factors likely to participate in their pathogenesis. Post stroke depression (PSD) is a common complication after stroke leading to poor functional outcome, increased physical disability and mortality. Although several genes have been associated with PSD, the genetic basis of PSD remains poorly understood. Method A 2-stage candidate gene study by targeted sequencing was conducted involving stroke patients with or without depression and health controls. In the discovery stage (121 PSD, 131 non-PSD and 639 HC), logistic regression was used to test associations respectively in PSD and non-PSD groups. In the replication stage (200 PSD, 218 non-PSD and 983 HC), 54 selected SNPs were again genotyped in an independent cohort. Fixed-effects inverse variance-weighted meta-analysis was used in the combined samples. Results The study identified 2 novel genes associated with PSD [HTR3D (rs55674402, p = 0.002512, odds ratio (OR) = 0.7431); NEUROG3 (rs144643855, p = 0.00325, OR = 0.6523)] and 3 risk SNPs in one risk gene associated with non-PSD [PIK3C2B (rs17406271, p = 0.0006801, OR = 1.446; rs2271419, p = 0.0005836, OR = 1.497; rs2271420, p = 0.001031, OR = 1.431)] in the Chinese sample. NEUROG3 shows highest expression level in hippocampus. Functional enrichment analysis shows that susceptibility genes for PSD are mostly enriched in chemical synaptic transmission and regulation of lipid synthetic process. Limitations The sample size was not sufficient to reach a genome-wide p value level. To overcome this shortage, some unique strategies were applied during the selection of SNPs for replication. Secondly, the age, gender composition and depressive severity between two stages were not well-matched. Different sample sources should be blamed, and to minimizing the influence, gender was corrected as co-variant in logistic regression. Conclusion This study identified that HTR3D and NEUROG3 were linked with the susceptibility of PSD and PIK3C2B with stroke in the Chinese Han population. Further replication of these findings in a larger and better matched sample is warranted. Functional analysis suggests that the pathogenesis of PSD may be implicated in 5-HT synaptic transmission, neural plasticity and lipid metabolism, and therapeutic interventions targeting these pathways may be effective approaches for PSD treatment.

In this study, five growth stages of Oriental lily ‘Justina’ leaves (10 d, 25 d, 50 d, 75 d and 100 d), and seven different parts of styles, anthers, petals, leaves, stems, bulbs and roots in 100 day growth were used as test materials to measure the content of carotenoid and chlorophyll in Lilium by organic solvent method. The semi-quantitative expression analysis of 12 key enzyme genes ( PSY , PDS , Z-ISO , ZDS , CRTISO , LCYB , LCYE , HYB , VDE , ZEP , CCS and NCED ) of Lilium carotenoid metabolic pathway was carried out according to the sequencing results of transcriptome library. The study cloned the open reading frame (ORF) sequence of Lilium LCYB gene and carried out related bioinformatics analysis. The results showed that carotenoid and chlorophyll content were significantly different in the five different stages of Lilium leaf growth and seven different parts of Lilium ripening stage. The expression levels of 12 key enzyme genes were variant in different growth stages and parts. The complete ORF of the LCYB gene was cloned with the whole length of 1,503 bp, encoding 500 amino acids, which had typical conserved region characteristics of Carotene Cycl super family. It showed the closest relationship with Musa acuminata among the known genome-wide plants. This study might provide a solid theoretical basis for further study of Lilium carotenoid metabolic pathways and further understanding of the molecular mechanism of Lilium color and leaf color synthesis.

This study was aimed to find out the law of hybridization breeding between triploid OT lily and diploid O lily, and breed interspecific hybrids. The conventional compression method was used to analyze maternal chromosome karyotypes. The cut style and normal stigma pollination methods were applied in cross hybridization between OT×O. The direct ovule inoculation method was used for embryo rescue at different time after pollination. In this study, 6 OT lilies as female parents were all triploids. The normal stigma pollination method was obviously better than the cut style method, the former fruit and seed setting rates were both evidently higher than the latter. In 24 OT(♀)×O(♂) hybridized combinations, 16 combinations could bear fruits accounting for 66.67%. Most of 16 combinations could generate plump seeds and the seed set rate was from 2.49% to 16.78%. 4 O(♀)×OT(♂) back-cross and 6 OT(♀)×OT(♂) self-cross combinations had no fruits. It was the most effective to do embryo rescue on the 60 d after pollination and the ovule germinating speed was the fastest with the emergence rate of 5.45%~18.56%. This study preliminarily revealed the law of hybridization breeding between triploid OT lily (♀) and diploid O lily (♂), which would lay the foundation for creating aneuploid variation and cultivating new breeds of interspecific hybrid.

Due to the arrival randomness of the ship to port, the disorderly power supply will further increase Peak-Valley difference of load in the distribution when the ship is powered by shore power, which is not conducive to the stability and economy of the grid operation. In order to solve the above problems, a distribution network dispatching architecture with distributed generation(DG) and shore power supply is proposed in this paper; in view of the distribution network scheduling system with ship power supply, a two-layer ordered power supply dispatching method is proposed, The power distribution index of the grid layer is optimal for the integrated load index to ensure the stability and economical efficiency of the power system when the ship is connected, the goal of the user layer station system is the fitness of power supply load to dispatching order, which aims to the orderly scheduling of marine power supply; At last, the power supply method is verified by the actual load data of a certain area in Liaoning, and the error of the power supply scheduling plan and the actual power supply load is less than 3%, which proves the effectiveness of the proposed method.