Search

Your search keyword '"Zeng, Feng"' showing total 2,176 results
Start Over Author "Zeng, Feng"
2,176 results on '"Zeng, Feng"'

171. Plant regeneration via protoplast electrofusion in cassava

Author

Feng WEN, Wen-pan SU, Hua ZHENG, Ben-chi YU, Zeng-feng MA, Peng ZHANG, and Wen-wu GUO

Subjects
tissue culture, chromosome counting, DNA loss, ploidy analysis, Agriculture (General), S1-972
Abstract

Protoplast electrofusion between callus protoplasts of cultivar TMS60444 and mesophyll protoplasts of cultivar SC8 was performed as an approach for the genetic improvement of cassava. The fusion products were subsequently cultured in protoplast culture medium (TM2G) with gradual dilution for approximately 1–2 months. Then the protoplast-derived compact calli were transferred to suspension culture medium (SH) for suspension culture. The cultured products developed successively into embryos, mature embryos, and shoots on somatic embryo emerging medium (MSN), embryo maturation medium (CMM), and shoot elongation medium (CEM), respectively. And the shoots were then rooted on Murashige and Skoog (1962) medium (MS). Sixty-six cell lines were obtained and 12 of them developed into plantlets. Based on assessment of ploidy level and chromosome counting, four of these plantlets were tetraploid and the remaining eight were diploid. Based on assessment of ploidy level and simple sequence repeat (SSR) analysis, nine tetraploid cell lines, one diploid variant plant line and nine variant cell lines were obtained. The diploid variant plant line and the nine variant cell lines all showed partial loss of genetic material compared to that of the parent TMS60444, based on SSR patterns. These results showed that some new germplasm of cassava were created. In this study, a protocol for protoplast electrofusion was developed and validated. Another important conclusion from this work is the confirmation of a viable protocol for the regeneration of plants from cassava protoplasts. Going forward, we hope to provide technical guidance for cassava tissue culture, and also provide some useful inspiration and reference for further genetic improvement of cassava.

Published
2020
Full Text
View/download PDF

172. Determinants and Assembly Mechanism of Bacterial Community Structure in Ningxia Section of the Yellow River

Author

Rui-Zhi Zhao, Wei-Jiang Zhang, Zeng-Feng Zhao, and Xiao-Cong Qiu

Subjects
Ningxia section of the Yellow River, bacterial community structure, co-occurrence patterns network, driving factors, assembly mechanism, Biology (General), QH301-705.5
Abstract

The Yellow River is a valuable resource in the Ningxia Hui Autonomous Region and plays a vital role in local human activities and biodiversity. Bacteria are a crucial component of river ecosystems, but the driving factors and assembly mechanisms of bacterial community structure in this region remain unclear. Herein, we documented the bacterial community composition, determinants, co-occurrence pattern, and assembly mechanism for surface water and sediment. In comparison to sediment, the bacterioplankton community showed significant seasonal variation, as well as less diversity and abundance. The network topology parameters indicated that the sediment bacterial network was more stable than water, but the bacterioplankton network had higher connectivity. In this lotic ecosystem, CODMn, Chl a, and pH affected the structure of the bacterioplankton community, while TP was the primary factor influencing the structure of the sediment bacterial community. The combined results of the neutral community model and the phylogenetic null model indicate that Bacterial communities in both habitats were mainly affected by stochastic processes, with ecological processes dominated by ecological drift for bacterioplankton and dispersal limitation for sediment bacteria. These results provide essential insights into future research on microbial ecology, environmental monitoring, and classified management in the Ningxia section of the Yellow River.

Published
2023
Full Text
View/download PDF

182. Synergistic Based Social Incentive Mechanism in Mobile Crowdsensing

Author

Liu, Can, Zeng, Feng, Li, Wenjia, Hutchison, David, Series Editor, Kanade, Takeo, Series Editor, Kittler, Josef, Series Editor, Kleinberg, Jon M., Series Editor, Mattern, Friedemann, Series Editor, Mitchell, John C., Series Editor, Naor, Moni, Series Editor, Pandu Rangan, C., Series Editor, Steffen, Bernhard, Series Editor, Terzopoulos, Demetri, Series Editor, Tygar, Doug, Series Editor, Weikum, Gerhard, Series Editor, Chellappan, Sriram, editor, Cheng, Wei, editor, and Li, Wei, editor

Published
2018
Full Text
View/download PDF

197. WFST primary mosaic CCD camera and its first light

Author

Holland, Andrew D., Minoglou, Kyriaki, Zhang, Hong-fei, Feng, Qi, Wang, Jian, Wang, Hui, Zhang, Jun, Zeng, Feng, Wang, Zhi-yue, Geng, Zhe, Zhang, Yi-hao, Zheng, Zhen-hao, Liu, Zi-yi, Jiang, Wei-jie, Wei, Jiang-yuan, Zhu, Zhe-yu, Shi, Xing, Zhu, Rui, Chen, Long-hu, Chen, Cheng, Chen, Jin-ting, Tang, Qi-jie, Zhu, Jie, Ge, Kun, Gao, Jie, and Liu, Hao

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results