151. Random Sampling Process Leads to Overestimation of β-Diversity of Microbial Communities
- Author
-
Jizhong Zhou, Yi-Huei Jiang, Ye Deng, Zhou Shi, Benjamin Yamin Zhou, Kai Xue, Liyou Wu, Zhili He, and Yunfeng Yang
- Subjects
Microbiology ,QR1-502 - Abstract
ABSTRACT The site-to-site variability in species composition, known as β-diversity, is crucial to understanding spatiotemporal patterns of species diversity and the mechanisms controlling community composition and structure. However, quantifying β-diversity in microbial ecology using sequencing-based technologies is a great challenge because of a high number of sequencing errors, bias, and poor reproducibility and quantification. Herein, based on general sampling theory, a mathematical framework is first developed for simulating the effects of random sampling processes on quantifying β-diversity when the community size is known or unknown. Also, using an analogous ball example under Poisson sampling with limited sampling efforts, the developed mathematical framework can exactly predict the low reproducibility among technically replicate samples from the same community of a certain species abundance distribution, which provides explicit evidences of random sampling processes as the main factor causing high percentages of technical variations. In addition, the predicted values under Poisson random sampling were highly consistent with the observed low percentages of operational taxonomic unit (OTU) overlap (
- Published
- 2013
- Full Text
- View/download PDF