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The Circular Electron Position Collider (CEPC) aims at precision measurements of Higgs, W and Z boson properties. High granularity calorimetry associated with Particle Flow Algorithm (PFA) aims at jet energy resolution around 30%/sE/GeV. One option for the Hadronic Calorimeter (HCAL) is the so-called Analog HCAL (AHCAL) which consists of stainless steel as the absorber and scintillators as the sensitive material. This talk presents the latest progress on the optimisation studies of the HCAL detector cell in terms of light output and uniformity. Simulation studies on the software compensation technique are also presented, which shows around 10% improvement of the energy resolution with hadrons. In addition, we tried out 8 iterations of polystyrene recipe prototyping and scintillator tiles production with the injection moulding technique and achieved scintillator uniformity within the same batch better than 90%. Effective and fast ways of integrating the detector cells are under development such as an automatic wrapping machine and a glue dispenser. Preliminary results have been made for a merged readout scheme, which may provide a feasible method to reduce the number of electronic channels.

In this paper we report results of experimental studies of the dependence of the proton beam intensity and proton fraction of the 2.45 GHz electron cyclotron resonance (ECR) ion source on the operational parameters such as extraction voltage, microwave window, impedance matching, gas flow, microwave power, pipeline gas pressure, plasma chamber size, and magnetic field. The DC hydrogen ion beams are measured and analyzed on the bench of ion source and pre-analysis system of the Lanzhou university high intensity neutron generator ZF400. Our results show that a higher proton beam intensity and fraction can be obtained with a microwave window made up of 2 mm AlN. The proton beam intensity and fraction strongly depend on the three-stub tuner that controls the impedance matching between microwave and plasma. We also observe notable beam loss under the lower vacuum pressure condition.

Chemotherapy and photo-sonodynamic therapy (PSDT) can be combined through drug delivery nano-platforms to enhance the anti-tumor efficacy, however, which is limited by hypoxia in tumor, thereby causing chemotherapy resistance. Perfluoropentane (PFP) has the ability to carry oxygen and to enhance ultrasound or photoacoustic imaging after vaporization. Herein, we constructed a kind of nanoparticles (PTX/ICG and oxygen loaded PLGA nanoparticles (PIO_NPs)), which had PFP core carrying oxygen and PLGA shell loaded indocyanine green (ICG) and paclitaxel (PTX). PIO_NPs harbored good optical stability and the ability to transit phase. Moreover, it could rapidly release PTX and generate ROS under the mediation by near-infrared laser and low-intensity ultrasound. The PIO_NPs enhanced contrast of the ultrasound and PA imaging. In particular, PIO_NPs may be used to monitor and guide treatment for the accumulation of PIO_NPs at tumor site can be observed by PA imaging. Compared with PTX or other nanoparticles, PIO_NPs combined with laser and ultrasound (L.U) significantly induced apoptosis of SKOV3 cells and inhibited SKOV3 tumor growth. Therefore, PIO_NPs are of great potential in cancer imaging and therapy.

Aegilops sharonensis (Sharon goatgrass) is a valuable source of novel high molecular weight glutenin subunits, resistance to wheat rust, powdery mildew, and insect pests. In this study, we successfully hybridized Ae. sharonensis as the pollen parent to common wheat and obtained backcross derivatives. F1 intergeneric hybrids were verified using morphological observation and cytological and molecular analyses. The phenotypes of the hybrid plants were intermediate between Ae. sharonensis and common wheat. Observations of mitosis in root tip cells and meiosis in pollen mother cells revealed that the F1 hybrids possessed 28 chromosomes. Chromosome pairing at metaphase I of the pollen mother cells in the F1 hybrid plants was low, and the meiotic configuration was 25.94 I + 1.03 II (rod). Two pairs of primers were screened out from 150 simple sequence repeat markers, and primer WMC634 was used to identified the presence of the genome of Ae. sharonensis. Sequencing results showed that the F1 hybrids contained the Ssh genome of Ae. sharonensis. The sodium dodecyl sulfate polyacrylamide gel electrophoresis profile showed that the alien high molecular weight glutenin subunits of Ae. sharonensis were transferred into the F1 and backcross derivatives. The new wheat- Ae. sharonensis derivatives that we have produced will be valuable for increasing resistance to various diseases of wheat and for improving the quality of bread wheat.

Transmission capacity is an important indicator to evaluate the reliability of a power grid. Spatial Characteristic Length Ratio (SCLR) is one of the transmission capacity indexes which combines the complex network theory into the power system and can reflect the transmission capacity. In this paper, take a complex network considering characteristics of power system, an improved topology network model of power system is proposed. The relationship between the network topology index and grid transmission capacity is defined and calculated. Some general rules for describing grid transmission capacity are obtained. The correctness and feasibility of the proposed method are proved by simulation.

Atrial Fibrillation (AF) is common in the elderly and Sestrins (Sesns) have been suggested to prevent age-related pathologies. The aim of this study was to investigate the effects of Sesns in AF. Clinical data were collected and a small sample of atrial appendage and atrium was obtained from patients undergoing valve repairment. The expression of Sesn1, Sesn2, and Sesn3 was significantly higher in patients with permanent atrial fibrillation (PmAF) than that in sinus rhythm (SR), and further greater in the left atrium than the right in PmAF patients. Superoxide anion and malondialdehyde were enhanced and positively correlated to the protein expression of Sesn1/2/3. Reactive oxygen species (ROS) production and Ca2+ overload were significantly decreased and cell survival was enhanced by overexpression of Sesns 1/2/3 in cultured HL-1 cells. Conversely, knockdown of Sesn1/2/3 resulted in significantly increased ROS and Ca2+ overload. In addition, the overexpression of Sesn1/2 significantly reduced the proliferation of fibroblasts, as well as decreased the protein expression of collagen and fibronectin1 in angiotensin II-stimulated cardiac fibroblasts. Our study demonstrated for the first time that Sesns expression is significantly up-regulated in AF, which therefore may protect hearts against oxidative damage and atrial fibrosis.

Tilapia (Oreochromis mossambicus, O. urolepis hornorum, their hybrids O. mossambicus♀ × O. hornorum♂ and O. hornorum♀ × O. mossambicus♂) were exposed to a high salinity environment to evaluate their osmoregulatory responses. The plasma osmolality of all the tilapia species were elevated with the salinity challenge. The activities of Na

Feasibility of rock dynamic properties by split-Hopkinson pressure bar (SHPB) was experimentally and numerically evaluated with ANSYS/LS-DYNA. The effects of different diameters, different loading rates, and different propagation distances on wave dispersion of input bars in SHPB with rectangle and half-sine wave loadings were analyzed. The results show that the dispersion effect on the diameter of input bar, loading rate, and propagation distance under half-sine waveform loading is ignorable compared with the rectangle wave loading. Moreover, the degrees of stress uniformity under rectangle and half-sine input wave loadings are compared in SHPB tests, and the time required for stress uniformity is calculated under different above-mentioned loadings. It is confirmed that the stress uniformity can be realized more easily using the half-sine pulse loading compared to the rectangle pulse loading, and this has significant advantages in the dynamic test of rock-like materials. Finally, the Holmquist-Johnson-Concrete constitutive model is introduced to simulate the failure mechanism and failure and fragmentation characteristics of rock under different strain rates. And the numerical results agree with that obtained from the experiment, which confirms the effectiveness of the model and the method.

This paper has applied battery pack technologies in battery system based on the characteristics of charging and discharging process and composed big battery pack from single battery cells with MSP430 as micro control unit (MCU). The paper has also designed the monitoring hardware system of main control unit, data monitoring module, as well as the monitoring software of upper computer of CAN communication technologies with collected voltage, current and temperature of battery. The upper software is designed by module process with real-time monitoring function in voltage and temperature. SOC on-line prediction can be obtained by the establishment of mathematical model of Liion battery based on Extended Kalman Filter algorithm (EKF), the accuracy of the prediction is about 2.7%. The algorithm can be applied in battery management system of Li-ion battery for electrical vehicles with the same application effect and accuracy.

Pre-harvest sprouting (PHS) causes significant yield loss and degrade the end-use quality of wheat, especially in regions with prolonged wet weather during the harvesting season. Unfortunately, the gene pool of Triticum durum (tetraploid durum wheat) has narrow genetic base for PHS resistance. Therefore, finding out new genetic resources from other wheat species to develop PHS resistance in durum wheat is of importance. A major PHS resistance QTL, Qphs.sicau-3B.1, was mapped on chromosome 3BL in a recombinant inbred line population derived from ‘CSCR6’ (Triticum spelta), a PHS resistant hexaploid wheat and ‘Lang’, a PHS susceptible Australian hexaploid wheat cultivar. This QTL, Qphs.sicau-3B.1, is positioned between DArT marker wPt-3107 and wPt-6785. Two SCAR markers (Ph3B.1 and Ph3B.2) were developed to track this major QTL and were used to assay a BC2F8 tetraploid population derived from a cross between the durum wheat ‘Bellaroi’ (PHS susceptible) and ‘CSCR6’ (PHS resistant). Phenotypic assay and marker-assisted selection revealed five stable tetraploid lines were highly PHS resistant. This study has successfully established that PHS-resistance QTL from hexaploid wheat could be efficiently introgressed into tetraploid durum wheat. This tetraploid wheat germplasm could be useful in developing PHS resistant durum cultivars with higher yield and good end-use quality.

A negative oxygen ion source is under development to produce O− and O2− beams used for a secondary ion mass spectrometer at Institute of Modern Physics (IMP), and both filament and radio frequency (RF) schemes are tested. The filament driven ion source, which was initially designed for H− production, has a 10-pole multicusp plasma chamber, two sets of virtual magnetic filters, and a 3-electrode extraction system. The RF scheme, which is improved by changing a RF back plate from the filament ion source, has an external planar spiral RF antenna behind an AlN window. The RF power system consists of a continuous wave (CW) 13.56 MHz/2 kW power supply, a capacitive automatching network and a water-cooled flat RF antenna made from a 6-mm copper tube. Oxygen and carbon dioxide gases are used to produce O− and O2− ion beams, and ion composition is analyzed by using a Wien filter. However, the lifetime of filaments sustains from several minutes to hours before fracture occurred while the RF one can work stably with no maintenance, and the experiment results will be given on O− ion production with two different technologies using our ion source test facility.A negative oxygen ion source is under development to produce O− and O2− beams used for a secondary ion mass spectrometer at Institute of Modern Physics (IMP), and both filament and radio frequency (RF) schemes are tested. The filament driven ion source, which was initially designed for H− production, has a 10-pole multicusp plasma chamber, two sets of virtual magnetic filters, and a 3-electrode extraction system. The RF scheme, which is improved by changing a RF back plate from the filament ion source, has an external planar spiral RF antenna behind an AlN window. The RF power system consists of a continuous wave (CW) 13.56 MHz/2 kW power supply, a capacitive automatching network and a water-cooled flat RF antenna made from a 6-mm copper tube. Oxygen and carbon dioxide gases are used to produce O− and O2− ion beams, and ion composition is analyzed by using a Wien filter. However, the lifetime of filaments sustains from several minutes to hours before fracture occurred while the RF one can work stably with...

The proposed Circular Electron Positron Collider (CEPC) will allow precision Higgs measurements. To meet the stringent physics requirements, its vertex detector will have to be constructed with the state-of-the-art pixel detector technologies that promise high spatial resolution, low power consumption and low material budget. We have conducted R&D based on the emerging CMOS pixel sensor technology and developed the first prototype JadePix-1. In this paper, we describe the sensor structures that are primarily designed to evaluate the impacts of diode geometry on charge collection. We present the detailed test results obtained with radioactive sources and electron beams, and the sensor performance before and after neutron irradiation up to 1013 1 MeV neq/cm2.

With environmental pressure and the development of smart grid, penetration of large-scale renewable energy to power system seems to be imperative. However, it encounters some obstacles because of intermittencies of Renewable Generations (RGs). So mitigating impacts of intermittent RGs is a key issue for development of power system and this study presents a review on recent researches referring the problem from both electrical technologies and human factors. Technologies of storage, advance automation, electric vehicles, demand side management, risk constraint power system management, policies of renewable energies and the future research directions of them were discussed by the bibliographical survey.

A short-term Economic Dispatch (ED) model with risk constraint for wind penetrated power systems was built to face the challenge of scheduling spinning reserves brought from wind energies. The proposed model utilizes the probability of spinning reserve shortage as measurement of system risk and evaluates the risk by an Unequal Probabilities Sampling based Monte Carlo (UPSMC) method. A Genetic Algorithm (GA) improved with Simulated Annealing (SA) strategy is presented as SAGA to solve the problem. By comparing simulation results under different wind penetrations and risk constraints, coal consumptions will not always decrease with wind penetration and risk constraint but for most times. In addition, unit risk benefit has a trend to increase with wind penetration and decrease with risk constraint while contribution of unit wind generation has the contrary character. Simulation results also show that the proposed sampling method could improve the sampling efficiency and the SAGA method had better performance than traditional GA.

liuyujiao2005@126.com Abstract Aiming at a nonlinear/non-Gaussian filter problem, the data processing of a single-yarn strength testing system, a filtering method based on the particle filter algorithm is put forward. This paper expounds the principle, and the working process and the procedures of particle filter. It discusses in detail the application of particle filter in the single-yarn strength testing, the modeling of testing system state equation, and the implementation of this algorithm in a project. It also indicates that particle filter algorithm used to process single-yarn strength test data achieves a good effect and satisfactory filtering precision by simulated tests. Finally, this paper predicts that particle filter will play an important role in general data processing and analyzing. The detection of the yarn quality is very important for cotton production. Yarn strength is the reflection of inner quality of yarn and is the necessary condition of yarn's processing performance and end-use. The unit of single-yarn strength is Newton (N) per centimeter (CN). By stretching testing with a single-yarn strength tester, the yarn's characteristic parameters will be obtained, and the physical index of breaking strength, breaking elongation, fracture strength, fracture time and strength tensile curves can be determined, so the quality of yarn will be ascertained (1) . This paper majors in the research of the parameter of the single-yarn strength of yarn. The strength of yarn will increase with increasing within a certain twist; however the strength of yarn will decrease when the twist exceeds the critical value. The relationship between yarn elongation and tension is often nonlinear. When the yarn quality is tested by single-yarn strength tester, the interference noise of test environment in the industrial field is non-Gaussian. So the processing of single-yarn strength testing data is a typical nonlinear and non-Gaussian filtering problem. The traditional digital filtering methods normally only filter out the caused by single reason and can't achieve satisfactory results for complex noise. In order to solve this problem, a large number of nonlinear recursive filtering algorithm such as extended Kalman filtering (EKF), the modified gain Kalman filter (MGEKF), U Kalman filter (UKF) algorithm come forth in recent years, and these filters are based on particular assumption to ensure the optimality. The actual data is usually very complex, and contains many factors such as non-Gaussian, nonlinear, high dimensional and noise; in this case, the Kalman filter generally can't get the analytical solution. This is a common problem and appears in many different kinds of fields (2-11) . Now, a new sequential Monte Carlo particle filter algorithm based on Bayesian principles has been paid more attention. The particle filter technology realize the recursive Bayesian filter with non- parametric Monte Carlo simulation method, which can fit to any state space model and the nonlinear systems which can't be expressed by traditional Kalman filter, and its accuracy approach the accuracy of optimal estimation (12) . This paper places emphasis on introducing the principle and method of the particle filter, and the application of the nonlinear data processing.

Approximately ten percent of male infertility is caused by non-obstructive azoospermia (NOA), but the etiologies of many NOA remain elusive. Recently, a genome-wide association study (GWAS) of NOA in Han Chinese men was conducted and only a few genetic variants associated with NOA were found, which might have resulted from genetic heterogeneity. However, those variants that lack genome-wide significance might still be essential for fertility. Functional analysis of genes surrounding these variants inDrosophilaidentified some spermatogenesis-essential genes. As a complementary method ofDrosophilascreening, SK1 backgroundSaccharomvces cerevisiaewas used as a model to screen meiosis-related genes from the NOA GWAS data in this study. After functional screening,GDA1(orthologous to humanENTPD6) was found to be a novel meiosis-related gene. The deletion ofGDA1resulted in the failure of yeast sporulation. Further investigations showed that Gda1p was important for pre-meiotic S phase entry. Interestingly, the meiotic role of Gda1p was dependent on its guanosine diphosphatase activity, but not it’s cytoplasmic, transmembrane or stem domains. These yeast data suggest thatENTPD6may be a novel meiosis-associated NOA-related gene and the yeast model provides a good approach to analyze GWAS results of NOA.

In this article, a new approximate proximal point method for minimizing the sum of two convex functions is introduce. It replaces the optimization problem in the proximal point algorithm by a series of subproblems whose objective functions are approximal functions to get the optimal solution of the original optimization problem. In one subproblem, the function with less nonlinearity is replaced by its linearly approximating function and in the next subproblem, the function with more nonlinearity is replaced by the maximum of two linear functions, and so on. Three numerical examples are given to illustrate the effectiveness of the present algorithm.

A new isoflavanone, 2,2′-epoxy-4′-methoxy-3,7-dihydroxyisoflavanone (1), and a new natural coumaranone, 2-hyroxy-2-(4′-methoxybenzyl)-6-methoxy-3-coumaranone (2), along with 26 known compounds, were first isolated from the trunk of Horsfieldia pandurifolia. Their structures were elucidated by the means of spectroscopic analysis. Compound 1 was assessed for its cytotoxicity against five human tumour lines (HL-60, SMMC-7721, A-549, MCF-7 and SW-480), and the result showed that it has no activity.

The acidophilic Fe-oxidizing and S-oxidizing bacterium YY2 was isolated from the acid drainage of a coalmine. Based on morphological and physiological characteristics and phylogenetic analysis, it was identified as Acidithiobacillus ferrooxidans. Significant differences were observed in the oxidation efficiency and cell morphology when YY2 was cultured in 9K medium with ferrous ion (Fe2+), elemental sulfur (S0), and pyrite as the sole energy source. YY2 exhibited marked Fe2+ oxidation activity; 44.2 g·L−1 FeSO4·7H2O was completely oxidized in 30 h, but the rates of S0 and pyrite oxidization were slower. After 20 days, the efficiencies of oxidizing 10 g·L−1 S0 and 10 g·L−1 pyrite were approximately 9.6% and 20%, respectively. Cells cultured in pyrite as substrate secreted more extracellular polymeric substances than they did when cultured in Fe2+ or S0. Additionally, 75% total sulfur removal and 86% pyritic sulfur removal was achieved in a sequencing batch reactor of biodesulfurization of coal.

To establish chronic periodontitis model in SD rats, and to investigate the effect of oral intervention on atherosclerosis.Fifty male SD rats were randomly divided into three groups, group A (normal control), group B (atherosclerosis,As) and group C (chronic periodontitis, CP). Group C was further divided into group C1 (natural process), group C2 (simple mechanical treatment), group C3 (systemic antibiotics), group C4-1 (teeth extraction) and group C4-2 (teeth extraction+systemic antibiotics), each group consisted of 7 rats. Every group received oral intervention. Serum interleukin (IL)- 6 levels were detected in five different time points (1, 3, 5, 7, 9 weeks after a successful modeling) by enzyme linked immunosorbent assay. All animals were killed after 24 weeks. Matrix metalloproteinase (MMP)- 2, 9 in the proximal aorta was detected by immuno histochemistry.The levels of serum IL-6 in groups B and C1 increased gradually with time and became significantly higher than that in group A (P0.01). Levels of serum IL-6 were increased gradually in each intervention group (C2, C3, C4-1, C4-2) and reached its peak at 5 weeks after modeling [C2:(62.3 ± 14.3) ng/L, C3:(58.2 ± 8.7) ng/L, C4-1:(127.0 ± 29.9) ng/L, C4-2:(120.6 ± 23.1) ng/L]. Compared with group B, group C4- 1 and C4- 2 increased most significantly (P0.01). Levels of serum IL- 6 decreased gradually. Eventually, group C2 [(28.6 ± 8.1) ng/L], C3 [(40.8 ± 15.1) ng/L] and C4-2 [(32.7 ± 11.1) ng/L] were significantly lower than group B (P0.05), and in group C2 IL- 6 was the lowest. Although levels of serum of IL-6 significantly decreased in group C4-1 [(72.8 ± 16.4) ng/L], but remained the highest. Immunohistochemistry showed that MMP-2, 9 were expressed in group B, C1 and C4-1, and significantly higher than in group A (183.0 ± 2.0, 181.3 ± 2.0), the gray value differences were statistically significant (P0.01). Group C4-1 (123.1 ± 2.9, 121.0 ± 3.2) was the strongest, group B (126.4 ± 2.0, 124.8 ± 2.8) and C1 (140.0 ± 2.2, 139.7 ± 3.2) were decreased (P0.01). While group C2(169.3 ± 3.4, 169.7 ± 2.3), C3 (149.0 ± 1.7, 145.1 ± 2.5) and C4-2 (157.7 ± 1.2, 155.8 ± 2.7) were significantly lower than group C1 (P0.01), and group C2 was close to normal.Periodontitis could increase the risk of atherosclerosis in rats with chronic periodontitis. Periodontal mechanical treatment and teeth extraction may increase the risk of As in the short time. However, the risk would gradually reduce in a long time.

Both construction-stage monitoring and operation-stage monitoring are very much needed for a major rock slope project. An operation-stage safety monitoring system with an automatic monitoring information system was designed on the basis of a construction-stage monitoring system for the shiplock slope of Wuqiangxi Hydropower Station in the south central China. Its establishing method, prominent features, and application results are briefly introduced in this paper. Its characteristic functions are as follows: joint monitoring of key parts of the slope, thunderproofing of both installed transducers and monitoring instruments, all-weather continuous monitoring of relative displacement between slope toe and shiplock, advanced management of automatic monitoring, fast visual inquiry and analysis of monitoring data, and preliminary decision support for slope safety evaluation and management. It has met the special demands of operation-stage monitoring and plays an important part in raising the flood-storage capacity of the dam and ensuring the safe operation of the shiplock.

Electric vehicles are the most potential new energy vehicles to solve the energy crisis and the environmental pollution, but pure electric vehicles have some problems, such as short driving distance and long charging time, and complicated structure is the development bottleneck to hybrid electric vehicles, but extended range electric vehicles make up for these deficiencies effectively. The range extender and battery of extended range electric vehicles constitute the whole power source; first of all, determining the basic parameters of each part, and control strategy decides the economy, dynamic property and emission performance of the fuel. In combination with the structural characteristics of extended range electric vehicles, putting forward genetic algorithm to optimize the control strategy, then using advisor software to conduct simulation studies.

To investigate the effect of periodontal mechanical treatment on serum interleukin-6 (IL-6) and carotid artery matrix metalloproteinase (MMP)-2 and MMP-9 expression in chronic periodontitis (CP) SD rats with atherosclerosis (As).Forty-four six-week-old male SD rats were randomly divided into three groups: control group (group A), As group (group B), As+CP group(group C). According to different periodontal interventions, group C was randomly subdivided into four groups: natural process group (C1), the periodontal mechanical treatment group (C2), the periodontal mechanical treatment+ local drugs group (C3), and the periodontal mechanical treatment+local and system drugs group (C4). Each group received the appropriate treatment and periodontal interventions. Serum IL-6 levels were determined by enzyme linked immunosorrbent assay (ELISA). MMP-2 and MMP-9 levels in the proximal aorta were examined by immunohistochemistry.The gray value of MMP-2 and MMP-9 was basically the same in all groups. Compared with group A, the gray value of MMP-2 and MMP-9 of group B and C were decreased. C1 group showed the formation of atherosclerotic plaque and fibrous cap. Compared with group B (126.4 ± 2.0, 124.8 ± 2.8) , the gray value of group C1 (101.3 ± 2.4, 101.2 ± 4.1) was significantly weaker (P0.05). The staining depth of MMP-2 and MMP-9 of groups C1, C2, C3 and C4 were sequentially decreased, and the differences of gray value were statistically significant(P0.05). The levels of serum IL-6 in groups B and C1 increased gradually with time and became significantly higher than that of group A (P0.01). The levels of serum IL-6 in groups C2, C3, and C4 increased gradually and reached the peak 5 weeks after the establishment of model (P0.001). After that, the levels of serum IL-6 decreased gradually and was lower than baseline. The levels of serum IL-6 in groups C3 and C4 were significantly lower than that in group C2 7 weeks after the establishment of model(P0.01).In rats with periodontitis and cardiovascular diseases, chronic periodontal inflammation may significantly increase the severity of As and promote the formation of atherosclerotic plaque. Mechanical periodontal therapy may cause short-term systemic inflammation and then reduce vascular inflammation in long term.With supplement use of local and systemic antibiotics, the mechanical periodontal therapy may get the vascular disease and systemic inflammation improved.

From the extracts of Dimocarpus longan Lour leaves, 2 unusual flavonol glycosides, quercetin 3-O-(3″-O-2‴-methyl-2‴-hydroxylethyl)-β-d-xyloside (1) and quercetin 3-O-(3″-O-2‴-methyl-2‴-hydroxylethyl)-α-l-rhamnopyranoside (2), as well as 10 known compounds including 2 flavonol glycosides, afzelin (3) and kaempferol-3-O-α-l-rhamnopyranoside (4), 2 flavans, ( − )-epicatechin (5) and proanthocyanidin A-2 (6), 3 triterpenoids, friedelin (7), epifriedelanol (8) and β-amyrin (9), a peptide, N-benzoylphenylalanyl-N-benzoylphenylalaninate (10), and 2 sterols, β-sitosterol (11) and daucosterol (12) were isolated and identified by using combination of mass spectrometry and various 1D and 2D NMR techniques. This is the first report of flavonoid glycosides possessing a 2-methyl-2-hydroxylethoxyl group in sugar moiety from D. longan.

Meiotic recombination is essential for fertility in most sexually reproducing species, but the molecular mechanisms underlying this process remain poorly understood in mammals. Here, we show that RNF20-mediated H2B ubiquitination is required for meiotic recombination. A germ cell-specific knockout of the H2B ubiquitination E3 ligase RNF20 results in complete male infertility. The Stra8-Rnf20-/- spermatocytes arrest at the pachytene stage because of impaired programmed double-strand break (DSB) repair. Further investigations reveal that the depletion of RNF20 in the germ cells affects chromatin relaxation, thus preventing programmed DSB repair factors from being recruited to proper positions on the chromatin. The gametogenetic defects of the H2B ubiquitination deficient cells could be partially rescued by forced chromatin relaxation. Taken together, our results demonstrate that RNF20/Bre1p-mediated H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation, and suggest an old drug may provide a new way to treat some oligo- or azoospermia patients with chromatin relaxation disorders.

In this study we analyzed the genetic diversities of Qinghai’s faba bean ( Vicia faba L.) germplasms based on AFLP techniques. The results showed that the number of effective alleles in landraces, foreign germplasms and released cultivar are 1.065 5±0.126 3, 1.063 4±0.143 0, and 1.065 9±0.165 1, while the Shannon’s information index are 0.095 4±0.144 9, 0.084 3±0.151 8, 0.074 2±0.167 9, respectively. The ratio of effective allele of landrace is the lowest (73.73%), while its ploy-ratio is the highest (44.51%). Whereas the ratio of effective allele of released cultivars is the highest (90.39%), while its ploy-ratio is the lowest (17.92%). The genetic distance is relatively bigger in the landraces and smaller in the foreign germplasms. Genetic structure analysis revealed that the 149 faba bean germplasms in Qinghai can be divided into 7 groups. In this study we have selected 40 germplasms as core germplasm collection which would represent more than 80% genetic information of the tested 149 germplsams based on the genetic diversity and Nei\'s gene diversity.It is obvious that the core germplasm collection germplasms might be used as parent materials for hybrid breeding in faba bean breeding program.

New types of Thick Gaseous Electron Multipliers (THGEMs) made of PCB-type substrates, including Ceramic, Kapton, PTFE, and in addition to FR-4, were developed for applications requiring low neutron absorption, scattering, and low natural radioactivity, such as the THGEM-based neutron detector, and the THGEM-based gaseous multiplier (GPM). Using Geant4 simulation, the result of the total neutron absorption and scattering ratio of bare substrate is Kapton(high) > FR-4 > Ceramic > PTFE (low). The Ceramic and PTFE substrates have lower ratios about 0.707% and 0.635% per layer(0.2 mm thick) respectively. The gamma-induced electron background is the reverse, Ceramic > PTFE > FR-4 > Kapton, from 1.5% to 0.7% at 1.0 MeV, which induces highest electron background in the range of 0~7 MeV. The natural radioactivity background was measured for these types of substrate samples. The Ceramic substrate has lower radioactivity, 232Th = 8.8±0.9 Bq/kg, 238U = 6.3±0.9 Bq/kg, and 40K is too low to be detected. Some THGEM samples were produced by these four types of substrates in the same shape: 0.2-mm thickness, 0.2-mm hole diameter, 0.6-mm pitch, and 70-μm rim. The performances of these new substrate THGEMs are promising. The effective gain is at the 104 level and the gain was stable during more than 100 hours continuous test. The energy resolution @ Ar+IsoB = 97:3 is at the level of 20% to 27%. These new substrate THGEMs are available in 200×200 mm2 size and even larger, some of them are applied to neutron flux monitor detector and gaseous photomultipliers (GPM).

Background Kernel length is an important target trait in barley (Hordeum vulgare L.) breeding programs. However, the number of known quantitative trait loci (QTLs) controlling kernel length is limited. In the present study, we aimed to identify major QTLs for kernel length, as well as putative candidate genes that might influence kernel length in wild barley. Results A recombinant inbred line (RIL) population derived from the barley cultivar Baudin (H. vulgare ssp. vulgare) and the long-kernel wild barley genotype Awcs276 (H.vulgare ssp. spontaneum) was evaluated at one location over three years. A high-density genetic linkage map was constructed using 1,832 genome-wide diversity array technology (DArT) markers, spanning a total of 927.07 cM with an average interval of approximately 0.49 cM. Two major QTLs for kernel length, LEN-3H and LEN-4H, were detected across environments and further validated in a second RIL population derived from Fleet (H. vulgare ssp. vulgare) and Awcs276. In addition, a systematic search of public databases identified four candidate genes and four categories of proteins related to LEN-3H and LEN-4H. Conclusions This study establishes a fundamental research platform for genomic studies and marker-assisted selection, since LEN-3H and LEN-4H could be used for accelerating progress in barley breeding programs that aim to improve kernel length. Electronic supplementary material The online version of this article (doi:10.1186/s12863-016-0438-6) contains supplementary material, which is available to authorized users.