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Colorectal cancer (CRC) is the third most common malignancy and ranks as the second leading cause of cancer-related deaths worldwide. Despite the improvements in CRC diagnosis and treatment approaches, a considerable proportion of CRC patients still suffers from poor prognosis due to late disease detections and lack of personalized disease managements. Recent evidences have not only provided important molecular insights into their mechanistic behaviors but also indicated that identification of cancer-specific long non-coding RNAs (LncRNAs) could benefit earlier disease detections and improve treatment outcomes in patients suffering from CRC. LncRNAs have raised extensive attentions as they participate in various hallmarks of CRC. The mechanistic evidence gleaned in the recent decade clearly reveals that lncRNAs exert their oncogenic roles by regulating autophagy, epigenetic modifications, enhancing stem phenotype and modifying tumor microenvironment. In view of their pleiotropic functional roles in malignant progression, and their frequently dysregulated expression in CRC patients, they have great potential to be reliable diagnostic and prognostic biomarkers, as well as therapeutic targets for CRC. In the present review, we will focus on the oncogenic roles of lncRNAs and related mechanisms in CRC as well as discuss their clinical potential in the early diagnosis, prognostic prediction and therapeutic translation in patients with this malignancy.

To improve the properties of mullite-based thermal insulation materials typically prepared by foam-casting method, samples were heat treated under carbon monoxide atmosphere created by a carbon bed at 600 °C and 800 °C, respectively. The effects of reduction process on microstructure, pore size distribution, phase evolution, cold crushing strength (CCS), and thermal conductivity of the resultant materials were systematically studied. The results showed that the formation of high aspect ratio mullite whiskers and small amounts of glassy phases among whiskers during the reduction process are demonstrated as the key factors in improving the properties of the resultant materials. The mechanical properties are determined to be significantly enhanced for the binding effect of glassy phase among whiskers with soaking duration gradually increased to 48 h. And the lower thermal conductivity of samples treated for 24–48 h are mainly due to the high connectivity and lowered size of pores from the accumulation of high aspect ratio whiskers. Compared with samples treated at 800 °C, both the highest CCS (4.2 MPa) and minimum thermal conductivity (0.15 W/m K) were obtained in the sample heat treated at 600 °C for 48 h. Hence, it was demonstrated that the use of low-temperature reduction process could enhance the mechanical and thermal insulation properties of mullite-based thermal insulation materials.

Research on turbulent drag reduction has been limited owing to the limitations of existing parameters used for the identification of vortex structures. As a new and more suitable vortex parameter, Liutex provides a novel research method for turbulence control. We used a channel with a transverse groove structure as the research model and employed large-eddy simulation method based on Liutex to study the influence and mechanism of the transverse groove structure on the watershed. The analysis revealed that the transverse groove structure significantly inhibited the vortex intensity, and this effect diminished with the increase of the normal height. Moreover, the transverse groove structure could maintain the stability of the flow vortex and restrain the hairpin vortex. The fundamental action mechanism of the groove structure pertained to slip condition of the bottom surface along the flow direction, which was essential for conveying the bottom fluid. Furthermore, the transverse groove structure could significantly reduce the overall increase in the vortex intensity in the transition layer, and the fluctuation of the vortex intensity in the logarithmic layer was lower under the action of the groove structure. This case can guide the application of the new vortex identification method in turbulence drag reduction.

The incidence of diabetes worldwide continues to rise, placing a huge economic and medical burden on human society. More than 90% of diabetic cases are type 2 diabetes (T2D). At present, the pathogenesis of T2D is not yet fully understood. Metabolomics uses high-resolution analytical techniques (typically NMR and MS) to help identify biomarkers associated with the risk of T2D and reveal potential pathogenesis. Many metabolites such as branched-chain amino acids (BCAAs), aromatic amino acids, glycine, 2-hydroxybutyric acid (2-HB), lysophosphatidylcholine (LPC) (18:2), and trehalose have proven to be biomarkers of T2D. Insulin resistance (IR) induced by BCAA in T2D mice is related to the activation of mammalian target of rapamycin (mTOR) and phosphorylation of insulin receptor substrate-1 (IRS1). Incomplete LCFA [Formula: see text]-oxidation promote acylcarnitine byproduct accumulation and stimulates proinflammatory NF[Formula: see text]B-related pathways to inhibit insulin action. Traditional Chinese Medicine (TCM) presents unique advantages in the treatment of T2D. Multiple metabolites and metabolic pathways have been identified in the treatment of TCM, providing valuable biomarkers and novel targets for drug therapy and pharmacological mechanism. Therefore, this paper reviews the modern achievements of metabolomics in T2D research and the progress of TCM management in recent years, in order to provide valuable information for related research.

Since the successful separation of graphene from its bulk counterpart, two-dimensional (2D) layered materials have become the focus of research for their exceptional properties. The layered hexagonal boron nitride (h-BN), for instance, offers good lubricity, electrical insulation, corrosion resistance, and chemical stability. In recent years, the wide-band-gap layered h-BN has been recognized for its broad application prospects in neutron detection and quantum information processing. In addition, it has become very important in the field of 2D crystals and van der Waals heterostructures due to its versatility as a substrate, encapsulation layer, and a tunneling barrier layer for various device applications. However, due to the poor adhesion between h-BN and substrate and its high preparation temperature, it is very difficult to prepare large-area and denseh-BN films. Therefore, the controllable synthesis of h-BN films has been the focus of research in recent years. In this paper, the preparation methods and applications of h-BN films on III–V compounds are systematically summarized, and the prospects are discussed.

PURPOSE Gliomas are life-threatening brain tumors, and the extent of surgical resection is one of the strongest influences on survival rate. However, the proper distinction of infiltrated tissue remains elusive. The aim of this study was to use multimodal analyses to demarcate peritumoral tissue (PT) from tumoral (TT) and healthy tissue (HT). METHODS A total of 40 patients with histologically confirmed glioma were recruited. We analyzed resting-state functional magnetic resonance imaging (rs-fMRI) using the voxel-based mean blood-oxygen-level-dependent (BOLD) signal and the corresponding structural MRI (s-MRI) alongside RNA sequencing, whole-exome sequencing, and histology results of biopsy samples obtained from PT, HT, and TT. RESULTS We demarcated a functionally defined PT area where the mean BOLD signal gradually decreased near the edge of the tumor and extended beyond the TT borders (as defined by s-MRI), which was confirmed on a case-by-case basis. Correspondingly, genetic analyses showed a gene expression pattern and mutational landscape of the PT that were distinct from that seen in HT and TT. The genetic characterization of PT relative to HT and TT converged with the MRI-defined PT zones. This was confirmed in three individual cases after additional histologic analysis. A wider PT was associated with a longer progression-free survival, which suggests PT might act as an intermediate area between TT and HT. CONCLUSION Combined multimodal imaging and genetic analyses can allow for an objective demarcation of the PT in glioma and a robust classification of the degree of infiltration of the PT. These findings could help improve both neurosurgical resection and radio-oncologic therapy.

Inactive components and safety hazards are two critical challenges in realizing high-energy lithium-ion batteries. Metal foil current collectors with high density are typically an integrated part of lithium-ion batteries yet deliver no capacity. Meanwhile, high-energy batteries can entail increased fire safety issues. Here we report a composite current collector design that simultaneously minimizes the ‘dead weight’ within the cell and improves fire safety. An ultralight polyimide-based current collector (9 μm thick, specific mass 1.54 mg cm−2) is prepared by sandwiching a polyimide embedded with triphenyl phosphate flame retardant between two superthin Cu layers (~500 nm). Compared to lithium-ion batteries assembled with the thinnest commercial metal foil current collectors (~6 µm), batteries equipped with our composite current collectors can realize a 16–26% improvement in specific energy and rapidly self-extinguish fires under extreme conditions such as short circuits and thermal runaway. Batteries need to be energy-dense as well as safe. Yi Cui and team develop an ultralight polyimide-based current collector with embedded fire retardants that enables lithium-ion batteries with much-enhanced safety and energy density.

Background Ischemic heart disease is an imperative cause of high morbidity and mortality globally. The cardiac ischemia/reperfusion damage occur in both reperfusion and ischemia. Objective In this exploration, we have planned to examine the cardio-protective action of phyllanthin against the myocardial ischemic-reperfusion injury in mice. Materials and methods The myocardial ischemic reperfusion injury (MI-RI) stimulated via coronary artery occlusion, followed by the 10 mg/kg of phyllanthin treatment. The serum cardiac markers and pro-inflammatory markers level was investigated by using the assay kits. The expressions of oxidative stress and inflammatory markers level were investigated by immunohistochemical analysis. Lipid peroxidation, antioxidant enzymes, and ATPase levels level was examined by standard methods. The expression of oxidative stress markers were inspected by the reverse transcription polymerase chain reaction technique. The heart histology was investigated microscopically. Results The phyllanthin treatment increased the body weight, and heart weight also diminished the infarct size in the MI/RI mice. Cardiac markers status was diminished and the blood pressure markers were augmented by the phyllanthin. Histological analysis revealed the protective role of phyllanthin. Suppressed lipid peroxidation and enhanced antioxidant enzymes were noted in the phyllanthin treated mice MI-RI mice. Phyllanthin appreciably suppressed the pro-inflammatory regulators that is, NF-αB p65, IL-6, IL-1β, and TNF-α and enhanced the antioxidant marker expressions. ATPase levels were improved by the phyllanthin in the MI-RI mice. Conclusion These novel findings were confirmed the therapeutic role of phyllanthin against the MI-RI in mice. Hence, it can be a promising agent to treat the MI-RI induced cardiac dysfunction.

The next-generation satellite-based augmentation system (SBAS) will follow the dual-frequency multi-constellation (DFMC) protocol and broadcast satellite clock-ephemeris corrections and integrity information via GPS L5-like signals to improve the accuracy and integrity for GNSS dual-frequency users. BeiDou Navigation Satellite System (BDS)-3 B1C/B2a dual-frequency users will obtain a higher positioning accuracy using DFMC clock-ephemeris corrections. This paper focuses on an approach for the generation of DFMC SBAS corrections and its preliminary results. Analyzing the signal-in-space ranging error characteristics of BDS-3 satellites reveals that it is impossible to obtain orbits that are more accurate than the broadcast orbits using regionally distributed SBAS monitoring stations. Therefore, a novel DFMC SBAS correction generation approach for BDS-3 satellites is proposed in which only clock offset corrections are calculated. The effectiveness of the proposed approach is validated using measurements from mainland China. By using clock corrections only, B1C/B2a dual-frequency users can obtain the best accuracy. The results of the BDS-3 B1C/B2a dual-frequency positioning experiment show that the user equivalent ranging error is reduced from 0.59 m to 0.37 m with clock corrections but is reduced to only 0.43 m with the addition of clock-ephemeris corrections. With clock corrections only, the 95% positioning errors are reduced from 3.57 m to 1.53 m and from 3.79 m to 2.24 m in the horizontal and vertical directions, respectively, indicating a 48% reduction in positioning errors compared to users without SBAS corrections; however, the error reduction is only 40% with clock-ephemeris corrections.

Background: Over half of patients with advanced cancer report appetite loss or anorexia. Previous studies have shown the benefit of acupuncture for cancer-related nausea and vomiting, but limited evidence exists for its role in appetite improvement. Our study aimed to evaluate the feasibility and safety of auricular acupuncture to improve appetite for cancer patients with advanced disease. Methods: We performed a two-arm parallel, pilot randomized controlled trial (RCT) of auricular acupuncture versus usual care control in patients with stage III or IV cancer who experienced appetite loss. The primary outcome was changed in the Simplified Nutritional Appetite Questionnaire (SNAQ; score range, 4–20) between two groups from baseline to weeks 2 and 4, with secondary outcomes including change in weight, as well as an additional evaluation at week 8 for durability of treatment effects. We used independent two-sample t-test for the change in mean score for each outcome during or after treatment. We assessed the interaction between time and treatment from baseline to weeks 2, 4, and 8 using mixed-effects models by ANOVA test. Results: We randomized 55 patients to auricular acupuncture (N=27) or control group (N=28). By week 4, the auricular acupuncture group had a significantly higher escalation in the SNAQ score than the control group compared with baseline [mean difference 3.69; 95% confidential interval (95% CI): 2.5, 4.8; P

Heavy metals and polycyclic aromatic hydrocarbons (PAHs) are the important contaminants in used mineral oil (UMO) and likely pose environmental risks in the processes of disposal and regeneration. However, little information on the concentrations and associated with the risk of heavy metals and PAHs in various types of UMO from different industries is available. In this study, the occurrence of heavy metals and PAHs in the UMOs from typical industries in China was investigated. The median concentrations of 12 metals were 0.1-28 mg/kg. PAHs were mainly composed of 2- and 3-ring compounds and the median concentrations of 16 PAHs were 0.03-7.6 mg/kg in different UMO. The cluster analysis showed that samples with high heavy metal and PAH concentrations mainly originated from machine manufacturing and vehicle industries. The random forest model suggested that the species and phase of UMO were the main influencing factors associated with heavy metal concentrations, and the relative importance was 71.1-19.9%, 100-30.7%, 42.3-20.7%, 38.0-11.8%, and 100-24.1% for Cu, Zn, Ni, Cr, and Pb models, respectively, while industry was the main influencing factor associated with PAHs in UMO, and the relative importance was 81.5-51.5%, 27.7-16.5%, 83.4-25.3%, 94.8-21.4%, and 53.3-16.1% for 2- to 6-ring models, respectively.

Solid-state Li–S batteries are attractive due to their high energy density and safety. However, it is unclear whether the concepts from liquid electrolytes are applicable in the solid state to improve battery performance. Here, we demonstrate that the nanoscale encapsulation concept based on Li2S@TiS2 core–shell particles, originally developed in liquid electrolytes, is effective in solid polymer electrolytes. Using in situ optical cell and sulfur K-edge X-ray absorption, we find that polysulfides form and are well-trapped inside individual particles by the nanoscale TiS2 encapsulation. This TiS2 encapsulation layer also functions to catalyze the oxidation reaction of Li2S to sulfur, even in solid-state electrolytes, proven by both experiments and density functional theory calculations. A high cell-level specific energy of 427 W·h·kg–1 is achieved by integrating the Li2S@TiS2 cathode with a poly­(ethylene oxide)-based electrolyte and a lithium metal anode. This study points to the fruitful direction of borrowing concepts from liquid electrolytes into solid-state batteries.

Recepteur d’origine nantais (RON) has been implicated in cell proliferation, metastasis, and chemoresistance of various human malignancies. The short-form RON (sf-RON) encoded by RON transcripts was overexpressed in gastric cancer tissues, but its regulatory functions remain illustrated. Here, we found that sf-RON promoted gastric cancer cell proliferation by enhancing glucose metabolism. Furthermore, sf-RON was proved to induce the β-catenin expression level through the AKT1/GSK3β signaling pathway. Meanwhile, the binding sites of β-catenin were identified in the promoter region of SIX1 and it was also demonstrated that β-catenin positively regulated SIX1 expression. SIX1 enhanced the promoter activity of key proteins in glucose metabolism, such as GLUT1 and LDHA. Results indicated that sf-RON regulated the cell proliferation and glucose metabolism of gastric cancer by participating in a sf-RON/β-catenin/SIX1 signaling axis and had significant implications for choosing the therapeutic target of gastric cancer. Electronic supplementary material The online version of this article (10.1007/s10565-020-09525-5) contains supplementary material, which is available to authorized users.

Aspect-based sentiment analysis (ABSA) aims to detect the targets (which are composed by continuous words), aspects and sentiment polarities in text. Published datasets from SemEval-2015 and SemEval-2016 reveal that a sentiment polarity depends on both the target and the aspect. However, most of the existing methods consider predicting sentiment polarities from either targets or aspects but not from both, thus they easily make wrong predictions on sentiment polarities. In particular, where the target is implicit, i.e., it does not appear in the given text, the methods predicting sentiment polarities from targets do not work. To tackle these limitations in ABSA, this paper proposes a novel method for target-aspect-sentiment joint detection. It relies on a pre-trained language model and can capture the dependence on both targets and aspects for sentiment prediction. Experimental results on the SemEval-2015 and SemEval-2016 restaurant datasets show that the proposed method achieves a high performance in detecting target-aspect-sentiment triples even for the implicit target cases; moreover, it even outperforms the state-of-the-art methods for those subtasks of target-aspect-sentiment detection that they are competent to.

Increasing evidences suggest that insufficient radiofrequency ablation (IRFA) can paradoxically promote tumor invasion and metastatic processes, whereas the effects of moderate hyperthermia on cancer progression are not well illustrated. Our study found that IRFA can increase the in vitro migration, invasion, and epithelial–mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells via induction of Snail, a master regulator of EMT events. Among measured miRNAs, IRFA can decrease the expression of miR-148a-5p in HCC cells. Whereas overexpression of miR-148a-5p can reverse IRFA-induced migration of HCC cells and upregulation of Snail, mechanistically overexpression of miR-148a-5p can directly target and decrease the expression of protein kinase ATM (ataxia telangiectasia mutated), which can increase protein stability of Snail. Collectively, our data suggest that IRFA can regulate the miR-148a-5p/ATM/Snail axis to trigger migration of HCC cells.

Municipal solid waste incineration fly ash (referred to as the fly ash) presents an important environmental problem in China today, but strategies for its treatment have yet to be widely studied and implemented. The currently available methods for the dechlorination of fly ash are not sufficient, given the amounts of fly ash produced each year. To increase the reuse fraction of fly ash as raw material for cement production, we propose an improved dechlorination method. Specifically, fly ash was leached with the hydrolysate of municipal solid waste leachate (HMSWL) to remove the water-insoluble chlorine. Three-step HMSWL leaching removed 94.3% of the total chlorine in fly ash, much more than the 82.7% that was removed through three-step ultrapure water (UW) leaching. X-ray diffraction indicated that three-step UW leaching could remove Cl mainly in the forms of KCl, NaCl, CaClOH and AlOCl, whereas three-step HMSWL leaching could further remove more water-insoluble Cl in the forms of AlOCl. In addition, the experimental results further suggested that the low pH of HMSWL (4.9) contributed little to the water-insoluble Cl removal, whereas the displacement of organic acid radicals (especially by the butyrate radical) was the major cause of water-insoluble Cl removal. Therefore, HMSWL rich in butyrate radical could be an ideal water substitute for fly ash dechlorination.

Rationale: Cisplatin derivatives are first-line chemotherapeutic agents for epithelial ovarian cancer. However, chemoresistance remains a major hurdle for successful therapy and the underlying molecular mechanisms are poorly understood at present. Methods: RNA sequencing of organoids (PDO) established from cisplatin-sensitive and -resistant ovarian cancer tissue samples was performed. Glucose metabolism, cell senescence, and chemosensitivity properties were subsequently examined. Immunoprecipitation, mass spectrometry, Fӧrster resonance energy transfer-fluorescence lifetime imaging (FRET-FLIM), luciferase reporter assay, ChIP and animal experiments were conducted to gain insights into the specific functions and mechanisms of action of the serine/threonine kinase, Aurora-A, in ovarian cancer. Results: Aurora-A levels were significantly enhanced in cisplatin-resistant PDO. Furthermore, Aurora-A promoted chemoresistance through suppression of cell senescence and induction of glucose metabolism in ovarian cancer organoids and cells. Mechanistically, Aurora-A bound directly to the transcription factor sex determining region Y-box 8 (SOX8) and phosphorylated the Ser327 site, in turn, regulating genes related to cell senescence and glycolysis, including hTERT, P16, LDHA and HK2, through enhancement of forkhead-box k1 (FOXK1) expression. Conclusions: Aurora-A regulates cell senescence and glucose metabolism to induce cisplatin resistance by participating in the SOX8/FOXK1 signaling axis in ovarian cancer. Our collective findings highlight a novel mechanism of cisplatin resistance and present potential therapeutic targets to overcome chemoresistance in ovarian cancer.

Metastable nanomaterials, such as single-atom and high-entropy systems, with exciting physical and chemical properties are increasingly important for next-generation technologies. Here, we developed a hydrogen-substituted graphdiyne-assisted ultrafast sparking synthesis (GAUSS) platform for the preparation of metastable nanomaterials. The GAUSS platform can reach an ultra-high reaction temperature of 3,286 K within 8 ms, a rate exceeding 10

Charlson Comorbidity Index (CCI) is a good predictor for hospitalization cost and mortality among patients with chronic disease. However, the impact of CCI on patients after colorectal cancer surgery is unclear. This study aims to investigate the influence of comorbidity assessed by CCI on length of stay, hospitalization costs, and in-hospital mortality in patients with colorectal cancer (CRC) who underwent surgical resection. This historical cohort study collected 10,271 adult inpatients for CRC undergoing resection surgery in 33 tertiary hospitals between January 2018 and December 2019. All patients were categorized by the CCI score into four classes: 0, 1,2, and ≥3. Linear regression was used for outcome indicators as continuous variables and logical regression for categorical variables. EmpowerStats software and R were used for data analysis. Of all 10,271 CRC patients, 51.72% had at least one comorbidity. Prevalence of metastatic solid tumor (19.68%, except colorectal cancer) and diabetes without complication (15.01%) were the major comorbidities. The highest average cost of hospitalization (86,761.88 CNY), length of stay (18.13 days), and in-hospital mortality (0.89%) were observed in patients with CCI score ≥3 compared to lower CCI scores (p < .001). Multivariate regression analysis showed that the CCI score was associated with hospitalization costs (β, 7340.46 [95% confidence interval (CI) (5710.06–8970.86)], p < .001), length of stay (β, 1.91[95%CI (1.52–2.30)], p < .001), and in-hospital mortality(odds ratio (OR),16.83[95%CI (2.23–126.88)], p = .0062) after adjusted basic clinical characteristics, especially when CCI score ≥3. Notably, the most specific complication associated with hospitalization costs and length of stay was metastatic solid tumor, while the most notable mortality-specific comorbidity was moderate or severe renal disease. The research work has discovered a strong link between CCI and clinical plus economic outcomes in patients with CRC who underwent surgical resection.

Metasurfaces that can simultaneously manipulate both amplitude and phase have garnered interest and have promising applications owing to their strong beam-steering ability; however, achieving a high maximum transmission while covering the full phase shift remains challenging. This paper proposes a chiral-structured meta-atom composed of two external cross-polarized patches and an internal coupling structure. It enables the independent modulation of the phase, amplitude, and polarization at large incidence angles and ensures a high maximum transmission with a complete phase shift enabled by the two internal rotation structures. The transmission phase and amplitude can be independently controlled by adjusting the geometry and rotation angle of the meta-atoms. The performance and feasibility of the method were verified using an ultra-thin high-order Bessel beam generator sample with a thickness of 2 mm (about λ0/11 at 14 GHz). This design can meet arbitrary requirements for extreme beam steering and has broad application prospects in the fields of electromagnetism and photonics.

Chemodynamic therapy (CDT) based on Fenton or Fenton-like reactions is an emerging cancer treatment that can both effectively fight cancer and reduce side effects on normal cells and tissues, and it has made important progress in cancer treatment. The catalytic efficiency of Fenton nanocatalysts(F-NCs) directly determines the anticancer effect of CDT. To learn more about this new type of therapy, this review summarizes the recent development of F-NCs that are responsive to tumor microenvironment (TME), and detailedly introduces their material design and action mechanism. Based on the deficiencies of them, some effective strategies to significantly improve the anticancer efficacy of F-NCs are highlighted, which mainly includes increasing the temperature and hydrogen peroxide concentration, reducing the pH, glutathione (GSH) content, and the dependence of F-NCs on acidic environment in the TME. It also discusses the differences between the effect of multi-mode therapy with external energy (light and ultrasound) and the single-mode therapy of CDT. Finally, the challenges encountered in the treatment process, the future development direction of F-NCs, and some suggestions are analyzed to promote CDT to enter the clinical stage in the near future. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s12951-022-01278-z.

Background Resistance to oxaliplatin is a major obstacle for the management of locally advanced and metastatic colon cancer (CC). Although long noncoding RNAs (lncRNAs) play key roles in CC, the relationships between lncRNAs and resistance to oxaliplatin have been poorly understood yet. Methods Chemo-sensitive and chemo-resistant organoids were established from colon cancer tissues of the oxaliplatin-sensitive or -resistant patients. Analysis of the patient cohort indicated that lnc-RP11-536 K7.3 had a potential oncogenic role in CC. Further, a series of functional in vitro and in vivo experiments were conducted to assess the effects of lnc-RP11-536 K7.3 on CC proliferation, glycolysis, and angiogenesis. RNA pull-down assay, luciferase reporter and fluorescent in situ hybridization assays were used to confirm the interactions between lnc-RP11-536 K7.3, SOX2 and their downstream target HIF-1α. Results In this study, we identified a novel lncRNA, lnc-RP11-536 K7.3, was associated with resistance to oxaliplatin and predicted a poor survival. Knockout of lnc-RP11-536 K7.3 inhibited the proliferation, glycolysis, and angiogenesis, whereas enhanced chemosensitivity in chemo-resistant organoids and CC cells both in vitro and in vivo. Furthermore, we found that lnc-RP11-536 K7.3 recruited SOX2 to transcriptionally activate USP7 mRNA expression. The accumulative USP7 resulted in deubiquitylation and stabilization of HIF-1α, thereby facilitating resistance to oxaliplatin. Conclusion In conclusion, our findings indicated that lnc-RP11-536 K7.3 could promote proliferation, glycolysis, angiogenesis, and chemo-resistance in CC by SOX2/USP7/HIF-1α signaling axis. This revealed a new insight into how lncRNA could regulate chemosensitivity and provide a potential therapeutic target for reversing resistance to oxaliplatin in the management of CC.

To satisfy the demands of civil aviation organizations and other users of satellite navigation systems for high-precision and high-integrity service performance, many countries and regions have established satellite-based augmentation systems (SBAS) referring to the Radio Technical Commission for Aeronautics (RTCA) service standards and agreements. The BeiDou SBAS (BDSBAS) provides both single-frequency service, which augments Global Positioning System (GPS) L1 C/A signal, and dual-frequency multi-constellation (DFMC) service, which augments BeiDou Navigation Satellite System (BDS) B1C and B2a dual frequency signals presently, meeting the requirements of the RTCA DO-229D protocol and the SBAS L5 DFMC protocol requirements, respectively. As one of the main error sources, the pseudorange bias errors of BDSBAS monitoring receivers were estimated and their effect on the performance of the BDSBAS service was analyzed. Based on the user algorithms of SBAS differential corrections and integrity information, the service accuracy, integrity, and availability of the BDSBAS were evaluated using real observation data. The results show that the maximum of monitoring receiver pseudorange bias errors between L1P and L1P/L2P can reach 1.57 m, which become the most important errors affecting the performance of the BDSBAS service. In addition, the results show that the pseudorange bias of GPS BlockIII is the smallest, while that of GPS BlockIIR is the largest. Compared with the positioning accuracy of the open service of the core constellation, the positioning accuracy of the BDSBAS service can be improved by approximately 47% and 36% for the RTCA service and DFMC service, respectively. For RTCA services, the protection limit (PL) calculated with the integrity information can 100% envelop the positioning error (PE) and no integrity risk event is detected. The service availability of BDSBAS for APV-I approach is approximately 98.8%, which is mainly affected by the availability of ionospheric grid corrections in the service marginal area. For DFMC service, the integrity risk is not detected either. The service availability for CAT-I approach is 100%. Improving the availability of ionospheric grid corrections is one of the important factors to improve service performance of BDSBAS RTCA service.

MoS

Background This study aims to develop an evidence-based clinical practice guideline of acupuncture in the treatment of patients with moderate and severe cancer pain. Methods The development of this guideline was triggered by a systematic review published in JAMA Oncology in 2020. We searched databases and websites for evidence on patient preferences and values, and other resources of using acupuncture for treatment of cancer pain. Recommendations were developed through a Delphi consensus of an international multidisciplinary panel including 13 western medicine oncologists, Chinese medicine/acupuncture clinical practitioners, and two patient representatives. The certainty of evidence, patient preferences and values, resources, and other factors were fully considered in formulating the recommendations. The Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach was employed to rate the certainty of evidence and the strength of recommendations. Results The guideline proposed three recommendations: (1) a strong recommendation for the treatment of acupuncture rather than no treatment to relieve pain in patients with moderate to severe cancer pain; (2) a weak recommendation for the combination treatments with acupuncture/acupressure to reduce pain intensity, decrease the opioid dose, and alleviate opioid-related side effects in moderate to severe cancer pain patients who are using analgesics; and (3) a strong recommendation for acupuncture in breast cancer patients to relieve their aromatase inhibitor-induced arthralgia. Conclusion This proposed guideline provides recommendations for the management of patients with cancer pain. The small sample sizes of evidence limit the strength of the recommendations and highlights the need for additional research.

Redox mediators (RMs) play a vital role in some liquid electrolyte-based electrochemical energy storage systems. However, the concept of redox mediator in solid-state batteries remains unexplored. Here, we selected a group of RM candidates and investigated their behaviors and roles in all-solid-state lithium-sulfur batteries (ASSLSBs). The soluble-type quinone-based RM (AQT) shows the most favorable redox potential and the best redox reversibility that functions well for lithium sulfide (Li2S) oxidation in solid polymer electrolytes. Accordingly, Li2S cathodes with AQT RMs present a significantly reduced energy barrier (average oxidation potential of 2.4 V) during initial charging at 0.1 C at 60 °C and the following discharge capacity of 1133 mAh gs-1. Using operando sulfur K-edge X-ray absorption spectroscopy, we directly tracked the sulfur speciation in ASSLSBs and proved that the solid-polysulfide-solid reaction of Li2S cathodes with RMs facilitated Li2S oxidation. In contrast, for bare Li2S cathodes, the solid-solid Li2S-sulfur direct conversion in the first charge cycle results in a high energy barrier for activation (charge to ∼4 V) and low sulfur utilization. The Li2S@AQT cell demonstrates superior cycling stability (average Coulombic efficiency 98.9% for 150 cycles) and rate capability owing to the effective AQT-enhanced Li-S reaction kinetics. This work reveals the evolution of sulfur species in ASSLSBs and realizes the fast Li-S reaction kinetics by designing an effective sulfur speciation pathway.

The Ka-band dual one-way measurements from Inter-Satellite Link (ISL) devices equipped on the third-generation Beidou Navigation Satellite System (BDS3) follow a time division multiple access (TDMA) structure and can calculate inter-satellite and satellite-ground clock offsets. L-band two-way satellite time and frequency transfer (TWSTFT) is also applied for time synchronization between satellites and ground master. We focus on a full-ISL clock offset estimation and prediction algorithm that estimates all satellite clock parameters simultaneously utilizing ISL clock observations and also synchronizes the constellation to the system time in Beidou Time (BDT) using Ka-band satellite-ground clock observations. We discuss the applications of this algorithm by assessing the clock performance of all BDS3 satellites equipped with a passive hydrogen maser (PHM) or rubidium atomic clock. After investigating the proper prediction model for each satellite, we use the full-ISL algorithm for 24-h clock predictions. The constant hardware delays in the ISL measurements are calibrated by comparing the derived clock parameters with TWSTFT measurements; the full-ISL clock products show high accuracy and continuity. The BDS3 PHMs and rubidium clocks both have a small clock rate drift of 10–20 s/s2. The frequency stability of the BDS3 PHMs and some rubidium clocks is approximately 6–9 × 10–15 at 1-day intervals. A linear model is suitable for these small-drift clocks, while a quadratic model is essential for the other rubidium clocks. Applying the full-ISL clock prediction method improves the RMS of the 24 h prediction error from 0.88 to 0.75 ns for PHMs and from 2.62 to 1.64 ns for rubidium clocks. The estimated ISL hardware delay STDs are less than 0.2 ns, and the prediction errors evaluated with TWSTFT clock observations are similar to those evaluated with Ka-band clock observations.

Drug resistance and potential cardiotoxicity severely limit the DOX-mediated chemotherapy in clinical. Multi-drug combination is conducive to the realization of multi-modal synergy at the molecular level, which is crucial in drug dose optimization and improvement of therapeutic effect. In this work, fluorescent biotinylated Au Nanoclusters as an active targeting carrier was developed to realize real-time biological imaging and dual-drug delivery simultaneously. DNA toxin doxorubicin (DOX) and tyrosinase inhibitor gefitinib (GEF) were selected as dual-drug models for the treatment of human non-small cell lung cancer. The in vitro and in vivo results showed that dual-drug combination suppressed cancer cell growth more efficiently than any single formula at the same concentrations. GEF can block signaling in target cancer cells with mutated and overactive EGFR, thereby inhibiting tumor growth and metastasis and promoting tumor cell apoptosis. Combined with DOX chemotherapy, it will effectively overcome the problem of DOX resistance. In addition, the dual-drug delivery system produced excellent synergistic therapeutic effects without extra adverse toxicities. It provides a reference for the design and clinical application of the dual-drug delivery system.

The prognostic value of tumor regression grade (TRG) in patients with locally advanced rectal cancer treated with neoadjuvant chemoradiation therapy has been explored extensively. However, whether TRG is predictive of outcome in colon cancer following preoperative chemotherapy has not been reported.A total of 276 colon cancer patients who had undergone preoperative chemotherapy and surgery in Fudan University Shanghai Cancer Center during the period March 2014 through November 2019 were recruited in this study. 113 (40.9%) and 163 (59.1%) patients were diagnosed with locally advanced colon cancer (LACC) and metastatic colon cancer (mCC) before preoperative chemotherapy, respectively. The TRG was divided into TRG0 (complete response), TRG1 (good response), TRG2 (moderate response), and TRG3 (poor response).Of the 276 patients 4.0% were TRG0, 5.4% were TRG1, 29.3% were TRG2, 61.2% were TRG3. TRG0 and TRG1 or TRG0, TRG1 and TRG2 were combined to simplify analysis due to limited sample size. In entire cohort, the 3-year overall survival for TRG0-1, TRG2, and TRG3 groups were 80.0%, 68.8% and 43.3% (P = .003). In LACC cohort, TRG was not associated with patients' prognosis, which largely resulted from limited outcome events. In mCC cohort, the 3-year overall survival for TRG0-1, TRG2, and TRG3 groups were 74.3%, 62.8% to 28.1% (P0.001). Multivariate analysis demonstrated that TRG was an independent prognostic factor for overall survival in both entire cohort and mCC cohort (TRG3 vs. TRG0-2).TRG is a prognostic factor in predicting long-term outcomes of mCC patients treated with preoperative chemotherapy.

In this paper, the Flight Motion Recognition Method (FMRM) based on Multivariate Phase Space Reconstruction (MPSR) and Approximate Entropy (ApEn) is proposed against the problem of complex rule representation and costly training in the current research of Flight Motion Recognition (FMR). Firstly, the cardinality of Phase Space Reconstruction (PSR) is unified, and the PSR sequence of height, roll angle and pitch angle is obtained respectively. Then, phase points at the same position of the three reconstructed sequences is merged based on Bayesian theory to generate the new fusion sequence where the maneuvering motion segment is extracted. Finally, various flight actions can be identified according to the numerical difference in sequence complexity of action segments which is qualitatively and quantitatively described by Recurrence Plot (RP) and ApEn. Verifying the effectiveness of FMRM through multiple real continuous flight data. The result shows that the FMRM can quickly and accurately identify different maneuvers, which lays good foundation for the follow-up evaluation.

Objective: To evaluate the effectiveness of Chinese Herbal Medicine (CHM) on leukopenia/neutropenia induced by chemotherapy in adults with colorectal cancer (CRC). Methods: Eight electronic databases were searched from their inception to June 2020. Randomized controlled trials with clarified sequence generation were qualified. Two reviewers independently conducted the screening and data extraction. Methodological quality was assessed using the Risk of Bias tool. RevMan 5.4 was applied to the meta-analysis. Results: Twenty-seven studies involving 1867 participants were qualified, of which 26 were included in the quantitative synthesis. Meta-analysis showed that CHM significantly reduced the incidence of leukopenia induced by chemotherapy (RR = 0.69; 95% CI 0.59-0.82), as well as the grade 3/4 leukopenia (RR = 0.71; 95% CI 0.55-0.90). Meanwhile,CHM decreased the occurrence of neutropenia (RR = 0.52, 95% CI 0.35-0.77), especially for the grades 3/4 neutropenia (RR = 0.42, 95% CI 0.27-0.64). Twenty-six of the included studies focused on the adverse events related to CHM. Conclusion: CHM may relieve neutropenia/leukopenia induced by chemotherapy in adults with colorectal cancer.

Purpose Capsid protein L2 is the minor capsid protein of human papillomavirus 16 (HPV16). Although L2-based vaccines were developed, the therapeutic effect of recombinant viral capsid protein L2 (rVL2) was still to be illustrated. Methods We used glucose uptake and lactate production assay to verify the inhibitory effect of rVL2 on the glucose metabolism in cervical cancer cells. Secondly, we performed gene-chip assay, RT-PCR, and Western blot to determine the role of ITGB7/C/EBPβ signaling pathway in rVL2-mediated glucose metabolism in vitro. Finally, we used an animal model to verify the function of rVL2 in cervical cancer. Results We found that rVL2 reduced glucose uptake and lactate production levels in cervical cancer cells, which caused the inhibition of cell proliferation. rVL2 decreased the expression levels of key metabolic enzymes, including GLUT1, LDHA, and ALDOA, to affect cell metabolism in cervical cancer cells by inhibiting ITGB7/C/EBPβ signaling pathway in vitro and in vivo. Conclusion These results demonstrated the vital role of rVL2 in the glycolysis-induced cell growth and proliferation via suppressing ITGB7/C/EBPβ signaling axis.

Intracranial solitary fibrous tumors/hemangiopericytomas (SFT/HPCs) are vascular tumors that have a high rate of local recurrence and extracranial metastases. Intradural extramedullary spinal dissemination of intracranial SFT/HPC is extremely rare. There is a paucity of data available to elucidate the molecular mechanisms of intraspinal dissemination of intracranial SFT/HPC. Herein, we presented a case of intracranial SFT/HPC with intraspinal metastasis. The resected tumor specimens were enrolled in a clinical sequencing program, including whole-exome and transcriptome sequencing. By comparing genomic sequencing data of the intracranial tumors with intraspinal metastasis, we established the somatic mutational profiles of these tumors. Clonality analysis revealed a distinct subclonal structure in the intracranial tumor and its intraspinal metastasis, which might reflect the possibility of intratumoral clonal selection and evolution during the process of tumor dissemination. Through bioinformatics analysis and Sanger sequencing validation, a DSTYK mutation (Met296Ile) was identified as a candidate driver of intraspinal metastasis in this SFT/HPC case. Further, an intracranial tumor-derived SFT/HPC cell line, HPC3, was established to explore the mechanisms of the DSTYK mutation in promoting SFT/HPC metastasis. Based on the HPC3 cell model, we found that the DSTYK mutation promoted cell migration and invasion of HPC3 cells via activation of ERK1/2 signaling, which was inhibited by the MEK/ERK inhibitor AZD6244. The DSTYK mutation was also shown to upregulate the expression of two metastasis-related molecules: MMP2 and MMP9 in HPC3 cells; however, this effect was attenuated by AZD6244 treatment. Therefore, the DSTYK mutation may activate ERK1/2/MMP2/9 signaling to promote tumor cell metastasis in SFT/HPC. In conclusion, our study revealed the potential role of DSTYK mutation in the regulation of intraspinal metastasis of SFT/HPC, which might provide new biological insights into this rare disease.

In view of the poor electronic conductivity and inferior structural stability, the direct application of Metal-Organic Frameworks (MOFs) as an electrode material is limited. Herein, the carboxylated multi-wall carbon nanotubes (MWCNT) are utilized as a substrate for the in-situ growth of bimetallic NiCo-based MOFs (NiCo-MOF). The flower-string-like NiCo-MOF/MWCNT composites are successfully synthesized using 4, 4′-biphenyldicarboxylic acid (BPDC) as a ligand via a simple solvothermal approach. In the composites, MWCNT is wrapped with 2D NiCo-MOF nanosheets through some bonds or affinity between MWCNT and NiCo-MOF. Meanwhile, the MWCNT presence does not affect the formation of the NiCo-MOF crystals. Compared with the hydrangea-like NiCo-MOF microspheres, the flower-string-like composite structures are more loosely assembled by NiCo-MOF nanosheets. Owing to the guiding effect of MWCNT on the NiCo-MOF growth, many well-dispersed flower-string-like structures can be achieved by adjusting MWCNT dosages. Due to the unique hierarchical structure and good synergistic effect, the NiCo-MOF/MWCNT composites exhibit excellent capacitance performances. The optimal composites demonstrate a large specific capacitance of 1010 F g−1 at 0.5 A g−1, a good rate capability (760 F g−1 even at 10 A g−1), a good cycling stability and reversibility with the capacitance retention of 828 F g−1 (100% of the initial capacitance) and coulombic efficiency of approximate 98% at 5 A g−1 after 3000 cycles, a low internal resistance and charge transfer resistance. The asymmetric supercapacitor assembled by NiCo-MOF/MWCNT composites as electrode materials has high energy density even at high power density, good rate capability, excellent cycling stability and reversibility. Hence, the as-synthesized NiCo-MOF/MWCNT composites are a promising high-performance electrode material for supercapacitors.

Terrestrial biogeochemical silicon (Si) and carbon (C) cycles couple through various processes, such as silicate weathering and the dynamics resulting from different phytolith assemblages. For example, small amounts of organic C (typically ranging from 0.2 to 5.8%) can be occluded during phytolith formation. Phytoliths play an important role in coupled Si and C cycles. In this study, we analyzed variations in C sequestration and the seasonal dynamics of phytoliths formed in different vegetation types in order to clarify the processes and characteristics of phytolith-occluded-carbon (PhytOC) cycles. Firstly, we measured the variation range of phytolith content in the litter and soil of different vegetation types at 11.87-151.90 and 1.81-14.72 g kg-1, respectively, while we measured the corresponding variation range of PhytOC content at 3.58-24.13 and 0.04-0.65 g kg-1, respectively. We also found that seasonal changes in phytolith and PhytOC content were significant (P < 0.01), both exhibiting a significant decreasing trend from litter to soil and from the surface soil to 0-60 cm of soil layers. Secondly, we measured the variation range of PhytOC storage in the litter and soil (0-60 cm) of different vegetation types at 1.26-6.89 and 28.24-75.2 t, respectively. Finally, our study determined the contribution of PhytOC storage in soil (0.42%) compared with conventionally recognized soil C sequestration storage (0.64%). The phytolith C pool is an important component of the forest ecosystem C pool, which plays a critical role in mitigating global warming.

The third generation of the BeiDou navigation satellite system (BDS-3) is a global navigation system, and is expected to be in full operation by 2020. High-precision orbits are a precondition for BDS-3 to provide a highly accurate service, which needs a global tracking and monitoring capability for the operational satellites. However, it is difficult for BDS to construct global ground monitoring stations. Fortunately, Ka-band Inter-Satellite Link (ISL) antennae fitted to the BDS-3 satellites can be used to extend the visible arc of the Medium Earth Orbit (MEO) satellites and to enhance the ground stations for orbit determination. This paper analyses the ISL-enhanced orbit determination for eight BDS-3 satellites, using the data from ten Chinese domestic stations and 13 international Global Navigation Satellite System (GNSS) Monitoring and Assessment System (iGMAS) overseas stations. The results show that the Three-Dimensional (3D) position Root Mean Square (RMS) error of the Overlapping Orbit Differences (OODs) is approximately 1 m when only ten regional stations are used. When the ISL measurements are added, the 3D position RMS error is decreased to 0·5 m, and the accuracy of the 24-hour orbit prediction can also be improved from 2 m to 0·7 m, which is even better than that of the orbits determined using globally distributed stations. It can be expected that with the subsequent launch of BDS-3 satellites and the increasing number of ISLs, the advantage of the ISL enhanced orbit determination will become more significant.