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Explicit algebraic expressions are first proposed to predict easily and precisely the thermal-expansion coefficients of unidirectional reinforced plastics. They are given as functions of the thermal and elastic properties of the constituent materials and of the fiber-volume fraction. They are derived by considering circular anisotropic fibers arranged in a hexagonal array in a matrix. Then, analytical expressions are derived for the thermal-expansion coefficients and curing stresses in filament-wound laminated composites under the assumption of elastic behavior and within the framework of laminated plate theory. Experiments on carbon-fiber/epoxy composite cylinders reinforced by helical and circumferential windings show good agreement with the calculated values. The residual stresses induced by curing are found not to be negligible compared with the low tensile strength transverse to the fibers. Such algebraic expressions for thermal coefficients, together with those for elastic moduli and failure criteri...

TSUCHIYA, S., YAMAGUCHI, Y., KOBAYASHI, Y., MINEGISHI, M., IMAIZUMI, M., SUZUKI, H., KONNO, T. and TADA, K. An Epstein-Barr Virus-Negative B Lymphoma Cell Line (THP-2) from a Burkitt's Lymphoma of a Japanese Patient. Tohoku J. exp. Med., 1983, 140 (4), 429-434 - An Esptein-Barr virus-negative lymphoma cell line, THP-2, was established from a Burkitt's lymphoma taken from a Japanese patient. THP-2 cells grew in single cell suspension with a doubling time of 24hr; the cells carried surface-bound μ-λ immunoglobulins and formed rosettes with IgG antibody-coated ox erythrocytes. THP-2 cells expressed B1, common acute lymphoblastic leukemia (ALL) (J5) and Ia-like antigens of their surface as defined by monoclonal antibodies. Epstein-Barr virus-associated nuclear antigen (EBNA) was not detected. The cell line THP-2 has been maintained for over 5 years. Among the markers examined, only common ALL antigen has been present on the cell surface of THP-2 for these 5 years.

A human leukemic cell line (THP-1) cultured from the blood of a boy with acute monocytic leukemia is described. This cell line had Fc and C3b receptors, but no surface or cytoplasmic immunoglobulins. HLA haplotypes of THP-1 were HLA-A2, -A9, -B5, -DRW1 and -DRW2. The monocytic nature of the cell line was characterized by: (1) the presence of alpha-naphthyl butyrate esterase activities which could be inhibited by NaF; (2) lysozyme production; (3) the phagocytosis of latex particles and sensitized sheep erythrocytes; and (4) the ability to restore T-lymphocyte response to Con A. The cells did not possess Epstein-Barr virus-associated nuclear antigen. These results indicate that THP-1 is a leukemia cell line with distinct monocytic markers. During culture, THP-1 maintained these monocytic characteristics for over 14 months.

In this study, after confirming the suppression of autonomic nervous function by isoflurane anesthesia using autonomic antagonists, we pharmacologically investigated the involvement of vasomotor and cardiac sympathetic functions in systolic blood pressure variability (SBPV) frequency components in conscious rats at rest and during exposure to low-ambient temperature (LT-exposure, 9°C for 90 min). Under unanesthesia, phentolamine administration (α-adrenoceptor antagonist, 10 mg/kg) decreased the mid-frequency component (MF 0.33–0.73 Hz) and inversely increased the high-frequency component (HF 1.3–2.5 Hz). The increased HF was suppressed by subsequent treatment with atenolol (β-adrenoceptor antagonist, 10 mg/kg), but not with atropine (muscarinic receptor antagonist, 10 mg/kg). Moreover, phentolamine administration after atenolol decreased MF, but did not increase HF. LT-exposure increased MF and HF; however, phentolamine pretreatment suppressed the increased MF during LT-exposure, and atenolol pretreatment dose-dependently decreased the increased HF. These results suggest that MF and HF of SBPV may reflect α-adrenoceptor-mediated vasomotor function and β-adrenoceptor-mediated cardiac sympathetic function, respectively, in the conscious state.

The proportion of T-cell subsets was normal in all patients with X-linked agammaglobulinemia except for an 8-month patient who showed a decrease in OKT8+ T-cell population. In two patients with hypogammaglobulinemia with IgM production, T-cell subsets were normal in distribution and the patients' B cells produced only IgM in culture with autologous T cells. A patient with common variable immunodeficiency showed no imbalance in distribution of T-cell subsets. Imbalance of regulatory T-cell subsets was found in a patient with DiGeorge syndrome and severe combined immunodeficiency.

A successful transplantation of soybean agglutinin (SBA) and sheep red blood cell (SRBC)-fractionated, maternal marrow in a patient with severe combined immunodeficiency (SCID) is reported. The engraftment of HLA-haplotype mismatched marrow cells was obtained without apparent graft versus host disease (GVHD). With immunological reconstitution the patient recovered from a BCG infection, which might have been caused by a BCG inoculation before his bone marrow transplantation.

Twin free face centered cubic (FCC) silver nanoparticles of nearly monodispersed particle size were prepared by simple ripening of multi-twined particles (MTP). The particle size was 12nm and the product yield was almost 100%. The growth mechanism was considered to be similar to Ostwald ripening except that it utilized chemical potential gap between MTP and FCC NPs. The kinetics of particle generation stage affected the final particle size and the possibility of controlling the final defectless particle size from 8–17 nm maintaining almost 100% product yield was achieved. The particle generation stage kinetics is considered to determine the ratio between FCC and MTPs before ripening, and consequently the final particle size.

TSUCHIYA, S., KOBAYASHI, Y., YAMAGUCHI, Y., IMAIZUMI, M., MINEGISHI, M., KONNO, T. and TADA, K. Two Non-T/Non-B Leukemia Cell Lines (THP-3-1 and THP-3-2) Established from a Patient at Different Stages of the Disease. Tohoku J. exp. Med., 1983, 140 (4), 443-444 - Two leukemia cell lines of different phenotypic expression were established from a single patient with non-T/non-B acute lymphocytic leukemia (ALL); one (THP-3-1) was derived from the peripheral blood taken on admission before chemotherapy and the other THP-3-2) during the terminal stage. Both THP-3-1 and THP-3-2 cells were positive for terminal deoxynucleotidyl transferase (TdT) and Ia-like antigen but negative for surface immunoglobulins (Ig), nuclear antigen of Epstein-Barr virus (EBNA) and receptors for sheep erythrocytes. However, there was a difference in the expression of common ALL antigen (J5) between the two cell lines. J5+ cells were found in only 5% of THP-3-1 and in 96% of THP-3-2.