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3,652 results on '"Yin Sheng"'

157. Native Top-Down Mass Spectrometry and Ion Mobility MS for Characterizing the Cobalt and Manganese Metal Binding of α-Synuclein Protein

Author

Wongkongkathep, Piriya, Han, Jong Yoon, Choi, Tae Su, Yin, Sheng, Kim, Hugh I, and Loo, Joseph A

Subjects
Analytical Chemistry, Chemical Sciences, Physical Chemistry, Bioengineering, Neurosciences, Neurological, Cobalt, Intrinsically Disordered Proteins, Manganese, Models, Molecular, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, alpha-Synuclein, Native mass spectrometry, Metal binding, Protein-ligand complex, Top-down mass spectrometry, Electron capture dissociation, Electrospray ionization, α-Synuclein, Medicinal and Biomolecular Chemistry, Physical Chemistry (incl. Structural), Analytical chemistry
Abstract

Structural characterization of intrinsically disordered proteins (IDPs) has been a major challenge in the field of protein science due to limited capabilities to obtain full-length high-resolution structures. Native ESI-MS with top-down MS was utilized to obtain structural features of protein-ligand binding for the Parkinson's disease-related protein, α-synuclein (αSyn), which is natively unstructured. Binding of heavy metals has been implicated in the accelerated formation of αSyn aggregation. Using high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry, native top-down MS with various fragmentation methods, including electron capture dissociation (ECD), collisional activated dissociation (CAD), and multistage tandem MS (MS3), deduced the binding sites of cobalt and manganese to the C-terminal region of the protein. Ion mobility MS (IM-MS) revealed a collapse toward compacted states of αSyn upon metal binding. The combination of native top-down MS and IM-MS provides structural information of protein-ligand interactions for intrinsically disordered proteins. Graphical Abstract ᅟ.

Published
2018

194. Rational Conversion of Imine Linkages to Amide Linkages in Covalent Organic Frameworks for Photocatalytic Oxidation with Enhanced Photostability.

Author

Xue, Rui, Liu, Yin‐Sheng, Wang, Ming‐Yue, Guo, Hao, Yang, Wu, Guo, Ji‐Xi, and Yang, Guo‐Yu

Subjects
PHOTOCATALYTIC oxidation, PHOTOCATALYSTS, BENZYLAMINES, BINDING energy, PHOTOCATALYSIS
Abstract

Covalent organic frameworks (COFs) and their applications in photocatalysis have been extensively studied, but the instability of imine‐linked COFs is an important factor limiting their performance. In this work, two imine‐linked COFs were successfully converted to amide‐linked COFs through post synthetic modification (PSM). The oxidized COFs presented lower binding energy to O2, exhibited higher photocatalytic activity for oxidation of thioethers and coupling of benzylamines with excellent stability. The present work can serve as a reliable reference for the development of novel highly active and stable COF‐based photocatalysts. [ABSTRACT FROM AUTHOR]

Published
2024
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196. Diterpenoids with Potent Anti-Psoriasis Activity from Euphorbia helioscopia L.

Author

Zhao, Zhen-Zhu, Liang, Xu-Bo, He, Hong-Juan, Xue, Gui-Min, Sun, Yan-Jun, Chen, Hui, Zhao, Yin-Sheng, Bian, Li-Na, Feng, Wei-Sheng, and Zheng, Xiao-Ke

Subjects
B cells, INHIBITION of cellular proliferation, DITERPENES, T cells, EUPHORBIA
Abstract

Psoriasis, an immune-mediated inflammatory skin disorder, seriously affects the quality of life of nearly four percent of the world population. Euphorbia helioscopia L. is the monarch constituent of Chinese ZeQi powder preparation for psoriasis, so it is necessary to illustrate its active ingredients. Thus, twenty-three diterpenoids, including seven new ones, were isolated from the whole herb of E. helioscopia L. Compounds 1 and 2, each featuring a 2,3-dicarboxylic functionality, are the first examples in the ent-2,3-sceo-atisane or the ent-2,3-sceo-abietane family. Extensive spectroscopic analysis (1D, 2D NMR, and HRMS data) and computational methods were used to confirm their structures and absolute configurations. According to the previous study and NMR data from the jatropha diterpenes obtained in this study, some efficient 1H NMR spectroscopic rules for assigning the relative configurations of 3α-benzyloxy-jatroph-11E-ene and 7,8-seco-3α-benzyloxy-jatropha-11E-ene were summarized. Moreover, the hyperproliferation of T cells and keratinocytes is considered a key pathophysiology of psoriasis. Anti-proliferative activities against induced T/B lymphocytes and HaCaT cells were tested, and IC50 values of some compounds ranged from 6.7 to 31.5 μM. Compounds 7 and 11 reduced the secretions of IFN-γ and IL-2 significantly. Further immunofluorescence experiments and a docking study with NF-κB P65 showed that compound 13 interfered with the proliferation of HaCaT cells by inhibiting the NF-κB P65 phosphorylation at the protein level. [ABSTRACT FROM AUTHOR]

Published
2024
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197. Prenylated dihydroflavones from the root barks of Morus alba.

Author

Zhu, Jun-Yu, Weng, Han-Zhuang, Tang, Di-Kai, Long, Jin-Chen, Tang, Zhuo-Ya, Chen, Ye, Yin, Sheng, and Tang, Gui-Hua

Subjects
WHITE mulberry, ELLAGIC acid, EDARAVONE, PHEOCHROMOCYTOMA, NEUROPROTECTIVE agents
Abstract

Three new prenylated dihydroflavones, moralbaflavones A–C (1–3), together with four known ones (4a/4b, 5, and 6) were isolated from the root barks of Morus alba L. Their structures including the absolute configurations were determined by the analysis of HRMS, NMR, and ECD data. The neuroprotective properties of these prenylated dihydroflavones were screened at the concentration of 10 µM in the sodium nitroprusside-induced rat pheochromocytoma PC-12 cells, and the results showed moralbaflavone C (3) possessed significant neuroprotective activity, being more potent than the positive control edaravone. [ABSTRACT FROM AUTHOR]

Published
2024
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