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Yibo Geng,1,* Yuliang Wu,2,* Cheng Xu,1 Tian Li,1 Liwei Zhang1,3 1Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Department of Neurosurgery, Qilu Children’s Hospital, Shandong University, Jinan, Shandong, People’s Republic of China; 3China National Clinical Research Center for Neurological Disease, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Liwei ZhangDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nan Si Huan West Road, Fengtai District, Beijing 100070, People’s Republic of ChinaTel +86-15101181174Fax +86-010-59976611Email zhangliweittyy@163.comBackground: Numerous studies suggest that long non-coding RNAs (lncRNAs) participate in the biological process of diverse malignancies, including glioma. Although many differentially expressed lncRNAs have been identified in glioma, to our best knowledge, the role of LINC00662 and its potential underlying mechanism in glioma progression remains unclear. This study aimed to explore the function and regulatory network of LINC00662 in glioma.Methods: Expressions of LINC00662, miR-34a-5p and lectin mannose-binding 2-like (LMAN2L) in glioma tissues were analyzed using The Cancer Genome Atlas Program (TCGA) and the Chinese Glioma Genome Atlas (CGGA) databases. Colony formation, Celltiter-Glo and BrdU (5-bromo-2ʹ-deoxyuridine) incorporation assays were used to detect cell proliferation in vitro. Xenograft mouse models were established to determine cell proliferation in vivo. Transwell and wound healing assay was used to detect cell migration. In addition, epithelial–mesenchymal transition (EMT) markers were detected by Western blot. Annexin V and 7-AAD were used to stain apoptotic cells. Interactions between miR-34a-5p and LINC00662 or the 3′-UTR of LMAN2L were predicted and determined by bioinformatics analysis, luciferase reporter assay and RNA immunoprecipitation (RIP) assays.Results: High LINC00662 level predicted poor overall survival of glioma patients. Functional studies revealed that suppression of LINC00662 remarkably inhibited cell proliferation, clonogenicity and EMT pathway. Mechanistically, LINC00662 sponged miR-34a-5p to regulate LMAN2L expression. Furthermore, miR-34a-5p inhibitor reversed the anti-proliferation and anti-migration effect of LINC00662 knockdown, which could be rescued by downregulation of LMAN2L in glioma cells.Conclusion: Our study was the first to report that LINC00662 acted as a competing endogenous RNA (ceRNA) to regulate glioma progression by targeting miR-34a-5p/LMAN2L axis, providing a new therapeutic target for glioma.Keywords: long non-coding RNA, LINC00662, miR-34a-5p, LMAN2L, glioma, epithelial–mesenchymal transition

Ethanolamine(EA) often occurs at a relatively high concentration within the inflamed gut of IBD patients. To investigate the role of EA in colonic inflammation and host-microbiome dysbiosis, thirty-six ICR mice were treated with 3% DSS for a week to generate acute intestinal inflammation and then supplied with 0μM, 500μM (LowEA), and 3000 μM (HighEA) in drinking water for two weeks, after that,16s RNA sequencing was applied in characterizing the changes in colonic microbiota driven by different EA levels. An inflamed colonic organoid model via 3% DSS treatment was also established for further verification of these in vivo findings.EA significantly reduced proximal colonic crypt depth but increased distal colonic villus height in HighEA group. The protein and mRNA expression of occludin and Reg3β, BD1, BD2, and MUC2were significantly up-regulated in EA treated groups. EA decreased mucosal inflammation-related cytokines levels (IL1, IL6, IL17, TNFα, and INFγ) and increased the significantly increased concentration of sIgA. Serum aspartate aminotransferase and alanine aminotransferase were significantly down-regulated in the highEA group. EA increased the relative abundance of Blautia, Roseburia, Lactobacillus, Faecalibaculum, Candidatus_Saccharimonas, Alloprevotella, and Lachnoclostridum.and thus microbial metabolic pathways including Oxidative phosphorylation, Lipopolysaccharide biosynthesis, Arginine and proline metabolism, Folate biosynthesis, and Biotin metabolism were more abundant in LowEA group than those in control. EA up-regulated the protein or mRNA expression of TLR4/MyD88 in colonic tissues and the DSS-treated colonic organoid model. This study firstly demonstrated that ethanolamine in altering host-microbiome dysbiosis, which may provide new insights into the role of dietary lipids in IBD.ImportanceInflammatory bowel disease (IBD) affects ~3.1 million people in the USA and is increasing in incidence worldwide. IBD pathogenesis has been associated with gut microbiome dysbiosis characterized as a decrease in gut microbial diversity. Extensive works have demonstrated the roles of dietary fiber, short-chain fatty acids, and aromatic amino acids in altering the composition of gut microbiota to restore immune homeostasis and alleviate inflammation via diverse mechanisms in IBD. However, little is known about essential sphingolipids like ethanolamine (EA), an essential compound in the CDP-ethanolamine pathway for phosphatidylethanolamine (PE) in both intestinal cells and bacteria. PE synthesis deficiency can ultimately result in a loss of membrane integrity and metabolic disorders in IBD. Our results demonstrate that ethanolamine could improve colonic barrier functions and inflammatory immunoreactions via shifting microbiome dysbiosis, which provides new insights into the role of dietary lipids in IBD.

In the process of high sulfur coal mining, with coal gas outburst, a large amount of highly toxic flammable hydrogen sulfide (H2S) gas is produced which threats miner’s health and pollutes the environment seriously. It is shown that adding an oxidizing agent in alkaline absorption liquid can obviously increase the removal rate of H2S in mine air in the experiment results, and there’s no the hydrogen sulfide gas evolution after oscillation of the absorbing tail liquid, the removal efficiency is very remarkable. It is also shown that surfactant can reduce the surface tension of the absorption liquid in the research, accelerate the process of mass transfer between gas-liquid two phases, and improve the absorption efficiency.

Glioblastoma multiforme (GBM) is the most common and aggressive primary intracranial tumor. The tumor metastases outside the CNS are rare, so therapeutic experience with these types of tumors is limited. We present a case of a 20-year-old female with a history of left temporal anaplastic oligoastrocytoma, who was found to have biopsy proven metastases tothe lung. In April 2014, the patient presented with intermittent headache for 4 months. Physical examinations confirmed no obvious abnormalities. Computed tomography (CT) scan and magnetic resonance image (MRI) scan of the brain showed a large mass with ring-like enhancement in left temporal lobe. She underwent craniotomy to resect the intracranial tumor. Thepathological investigation showed the lesion to be a classical anaplastic oligoastrocytoma with focal necrosis and a Ki-67 labeling index of 10-30%. After operation, the patient received radiotherapy and chemotherapy. Two years later, the patient readmitted due to chest discomfort, a chest X-ray and CT showed a mass in the right lung. The lesion was confirmed to be a metastatic malignant glioma viapulmonary bronchoscopy biopsy. In conclusion, as the life expectancy is gradually increasing for GBM patients with newer therapies, the incidence of extracranial metastases may increase and this rare phenomenon may become more common and clinically relevant.