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This article presents the outcome of research on modelling the process of the extrusion of crystalline dry ice. The purpose of this process is to densify the material and obtain pellets of several millimeters in diameter. This reduces the sublimation rate in ambient conditions of the material whose temperature in a solid state is 195 K. A lower sublimation rate means a reduction of the loss of product in its final applications, which include refrigeration and reduction of atmospheric emissions of gaseous CO 2 . A ram-type extruder was considered in this analysis, in which dry ice was extruded through a single-hole die of varying geometry. The article presents the results of numerical analyses of the extrusion process, using a simulation method based on the Smoothed Particle Hydrodynamics (SPH) approach. The results from simulations were verified by the experimental data in terms of the maximum force required to complete the process, in order to assess the applicability of the proposed method in further research on dry ice compression.

Background and Objective: The main goal of the proposed study is to improve the efficiency of the ear treatment via targeted drug delivery to the inner ear, i.e. the cochlea. Although pharmacotherapy has been proposed as a solution to prevent damage or restore functionality to hair cells, the main challenge in such treatments is ensuring adequate drug delivery to the cells. To this end, we present a methodology for the evaluation of the magnetic forces needed to move magnetic particle nanorobots (abbreviated as MNP) and their aggregates through the cochlea round window membrane (RWM)., Methods: The FEM - Lagrangian-Eulerian approach (Abaqus software) was used to determine the specific parameters of movement of the nanoparticles crossing the RWM. This method results in a high consistency of FEM simulations and in-vivo experimental results in regards to the required magnetic force during the movement of spherical nanoparticles with a given viscosity η ave . Based on the analysis of the experimental studies found in subject literature, the sizes of the MNPs and their aggregates able to cross RWM with and without the application of magnetic force F M have been determined., Results: The present work accounts for both the experimental and theoretical aspects of these investigations. Presented research confirms the definite usability of the Lagrange-Euler method for the precise determination of the required magnetic force value FM to control the accelerated motion of MNP aggregates of complex shapes through RWM. It is possible to determine the predominant parameters with a precision of less than 5% for single-layer aggregates and spatial aggregates crossing the RWM. It can be concluded that the MNPs and their aggregates should not be larger than 500-750 nm to cross the RWM with high velocities of penetration close to 800 nm/s for magnetic forces of hundreds 10 -14 Newtons., Conclusions: The proposed Lagrangian-Eulerian approach is capable of accurately predicting the movement parameters of MNP aggregates of irregular shape that are close to the experimental test cases. The presented method can serve as a supplementary tool for the design of drug delivery systems to the inner ear using MNPs., Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest was present. The funders had no influence over the preparation of the study, the collection, analyses, or interpretation of data herein as well as on in the writing of the present manuscript as well as on the decision to publish the results., (Copyright © 2022. Published by Elsevier B.V.)

In the processing of waste materials, attention must be given to the efficient use of energy. The pelletization of dry ice is a good example of such processes. A literature review shows that in the pelletizers available on the market, the force applied in the process is excessive. As a result, the efficiency of the utilization of inputs, including electricity and carbon dioxide, is at a very low level. This article presents the results of experimental research on the effect of the degree of dry ice compression on the value of the Poisson ratio. The first part of this article presents the research methodology and a description of the test stand, developed specifically for this research, bearing in mind the unique properties of carbon dioxide in the solid state. The results presented show the behavior of dry ice during compression in a rectangular chamber for different final densities of the finished product. As a result, it is possible to determine the values of the Poisson ratio as a function of density, using for this purpose four mathematical models. The findings of this research may be useful for research work focused on the further development of this process, such as by using the Drucker-Prager/Cap numerical model to optimize the geometric parameters of the parts and components of the main unit of the machine used in the extrusion process of dry ice.

The objective of this paper is to analyze the belt punching process with the use of a single cutting edge and discuss the influence of geometrical features of the piercing punch on the perforation force. Two basic shapes of the piercing punch with a single cutting edge were tested: tools with the blade pointing inside or pointing outside. The analytical models of the stress distribution in the shearing cross sections were derived for both punches. The presented model, along with the series of empirical tests and Coupled Eulerian-Lagrangian simulation, was used for finding the effective geometry of the piercing punch with a single cutting edge for the belt perforation. The geometrical parameters taken into consideration for the tool optimization were the following: angle of the blade, thickness of the wall and diameter of the piercing punch cutting edge. The obtained results show that changing these parameters has a significant influence on the perforation force necessary to execute the machining process and affects the quality of the holes in the perforated belts. The most important geometrical features of the hollow sharpened punch are the angle and the direction of the blade, which change the force distribution and, as a result, the mechanics of the process.

Materials characterized by magnetorheological properties are non-classic engineering materials. A significant increase in the interest of the scientific community about this group of materials could be observed over the recent years. The results of research presented in this article are oriented on the examination of the said materials' mechanical properties. Stress relaxation tests were carried out on cylindrical samples of magnetorheological elastomers loaded with compressive stress, for various values of magnetic induction (B1 = 0 mT, B2 = 32 mT, B3 = 48 mT, and B4 = 64 mT) and temperature (T1 = 25 °C, T2 = 30 °C, and T3 = 40 °C). The results of these tests indicate that the stiffness of the examined samples increased along with the increase of magnetic field induction, and decreased along with the increase of temperature. On this basis, it has been determined that: the biggest stress amplitude change, caused by the influence of magnetic field, was σ0ΔB = 12.7%, and the biggest stress amplitude change, caused by the influence of temperature, was σ0ΔT = 11.3%. As a result of applying a mathematical model, it was indicated that the stress relaxation in the examined magnetorheological elastomer, for the adopted time range (t = 3600 s), had a hyperbolic decline nature. The collected test results point to the examined materials being characterized by extensive rheological properties, which leads to the conclusion that it is necessary to conduct further tests in this area.

The article presents a formulated mathematical model that enables the determination of the required compressive force in the extrusion process of dry ice employing multichannel dies. This is the main parameter in the piston-based dry ice extrusion process. The indicated model was developed for the purpose of further improvement of the energy efficiency of this extrusion process. It allows for the determination of the value of compressive force by accounting for 12 variables related to the geometrical parameters of the die and the physical characteristics of dry ice. Furthermore, the paper also provides descriptions of the empirical study methodologies together with the results. These were carried out in order to determine the difference between the results of mathematical modeling and actual measurement results. The final part of the article presents the results of the analysis of the mathematical model's sensitivity to the change of the physical characteristics of dry ice. The formulated tool may be employed to adapt the geometric parameters of the die in order to obtain the desired compressive force value and dry ice granulation with reduced energy consumption.

Purpose: Chronic Achilles tendon tears, including chronic ruptures with end gap over 6 cm making end-to-end suturing impossible, can be treated with autologous hamstring graft reconstruction. The primary goal of this study was to present the biomechanical and long-term clinical results of recently developed minimally invasive Achilles tendon reconstruction technique., Methods: Minimally invasive Achilles tendon reconstruction was applied to 8 foot and ankle cadaveric specimens as well as 18 patients with chronic Achilles tendon tears. Repaired cadavers were subjected to the biomechanical testing using a cyclic loading protocol. Patients with reconstructed Achilles tendon were subjected to the clinical, functional and isokinetic tests at 12 months after the treatment., Results: All of tested Achilles cadaveric specimens survived 2 loading blocks (250 cycles of 10-100 N load followed by additional 250 cycles of 10-200 N load). With three specimens, it was possible to perform the third cyclic loading block with 20-300 N load and two specimens survived the fourth block with 20-400 N load. Therefore, a mean number of 838 cycles (±178) within the range of 509-1000 was recorded. Two specimens which survived all 1000 cycles were pulled to failure at 25 mm/s rate. The results obtained in the load to failure testing were as follows: 398 N and 608 N of maximum load. The results of functional heel rise endurance test and single leg hop for distance test indicated a decrease in the endurance and strength of the injured limb. However, the results of the weight-bearing lunge tests indicated no tendency for elongation of the Achilles tendon. A comparative analysis of the isokinetic test results for the non-injured and injured limb was revealed no statistically significant differences for every isokinetic test (n. s.), with significant difference for isometric strength parameters (p = 0.0006)., Conclusions: The results of the biomechanical tests as well as 1-year extensive functional, clinical and isokinetic results of the minimally invasive technique for chronic Achilles tendon tears are encouraging. Patients returned to their normal physical activity, including sport pre-injury level in most cases., Level of Evidence: III.

Background: Studies conducted on human cell culture models have demonstrated that collagen-derived peptides can exert a beneficial effect in medicine. However, all these studies were conducted using animal collagen samples, most often originating from bovine or porcine skin. Currently attempts are being made to replace animal collagen with fish collagen., Objectives: The aim of the study was to compare the effect of silver carp skin-derived peptide extract on the transcriptional activities of human VEGF and hsp70.1 gene promoters inserted into the plasmids with secreted alkaline phosphatase as a reporter gene., Material and Methods: Changes in the activity of the promoters were investigated using a HEK293FT cell line transfected with pVEGF-SEAP or pHsp70-SEAP. The cells were cultured in dishes containing peptides separated using reverse-phase high performance liquid chromatography., Results: The study demonstrated that the silver carp skin-derived peptide extract exerts both an inhibitory effect on the VEGF gene promoter and activating effect on the hsp70.1 gene promoter. Higher biological activity was recorded in the case of a freshly prepared peptide extract compared to one stored at 4°C for three months., Conclusions: The silver carp skin-derived collagen peptides influence VEGF and hsp70.1 gene promoters' transcriptional activity.

Background: Low-temperature techniques that prevent protein denaturation are being used to extract collagen from fish skin for cosmetic purposes. These extracts contain collagen with its triple helix structure preserved, as well as a number of other proteins., Objectives: The aim of the study was to investigate collagen extracts from the skin of silver carp for the presence of small-molecule peptides., Material and Methods: Liquid chromatography-mass spectrometry (HPLC-MS) was performed to analyze collagen extracts from silver carp skin for the presence of small-molecule peptides., Results: A large number of different peptides were detected in the silver carp skin collagen extracts analyzed. Among the smaller peptides, the most abundant were those of 7-29 aminoacids originating from the following proteins: collagen Iα1, collagen Iα2, collagen Iα3, collagen VIα3, decorin, lumican, histone H2A, histone H2B and histone H4., Conclusions: The study demonstrated that, in addition to high-molecular-weight collagen proteins, acidic collagen extracts acquired from the skin of silver carp at temperatures up to 16°C also contain considerable amounts of small 7-29 amino-acid peptides. The application of these peptides could therefore be expected to result in beneficial clinical effects in patients in need of reconstructive treatment.

A potential reason for poor survival among patients with muscle-invasive bladder cancer (MIBC) in Poland is initial disqualification from curative treatment due to advanced stage of the disease or low performance status. The aim of this study was to describe patterns of care in patients with newly diagnosed MIBC. This is a multicentre retrospective cohort study involving 296 consecutive patients with primary histologically diagnosed MIBC. Therapeutic decisions and potentially underlying clinical factors were analysed. Full clinical data was available for 285 patients. One hundred and sixty-four (57.5%) patients were qualified for radical cystectomy (RC), 32 (11.2%) patients for a second step of transurethral resection of the bladder tumour (TURBT) intentionally followed by systemic chemotherapy, four (1.4%) patients after complete TURBT were qualified for adjuvant intravesical chemotherapy only, while the remaining 85 (29.8%) patients were qualified for palliative treatment in the form of chemotherapy and/or radiotherapy and/or best supportive care. Patients disqualified from curative treatment were older (78 vs. 69 years, p < 0.02), had lower BMI values (24.5 vs. 25.7 kg/m(2), p < 0.02), lower haemoglobin concentration (11.6 vs. 12.9 mg/l, p < 0.02), declared lower rate of nicotine abuse (50.5% vs. 72.1%, p < 0.02), and had a shorter time interval between first symptom and diagnosis (30 vs. 60 days, p = 0.02). As the majority of Polish patients with primary MIBC receive curative treatment, the stage of the disease alone seems not to be the leading cause of poor survival. However, appropriateness of qualification for RC and treatment quality needs to be assessed for final conclusion on the factors influencing outcomes of treatment in Poland.

Introduction: The aim of the study was to assess the waiting time, from establishing the indications for radical cystectomy to surgery, in patients with urothelial carcinoma of the bladder at different Polish urological centres and to determine its influencing factors., Material and Methods: Retrospective analysis of data was performed on all consecutive radical cystectomies, performed in 2008-2012, at 10 Polish urological centres. The waiting time of patients from establishing the indications for radical cystectomy to surgery, as well as factors potentially influencing this time, were assessed. University (3), provincial (3) and regional (4) hospitals were defined as the 3(rd), 2(nd) and 1(st) level referral hospitals, respectively., Results: A total of 575 patients qualified for radical cystectomy due to muscle invasive urothelial carcinoma of the bladder (MIBC, 68% of cases) or failure of previous treatment of non-muscle invasive urothelial carcinoma of the bladder (NMIBC, 32%) were included in the analysis. The average time after the establishment of indications to surgery was 73.4 days, with a median of 56 days. In the case of 121 patients (22.1%), the waiting time exceeded 90 days. Significant differences in waiting time were found when the hospital referral levels were taken into consideration. In the 3(rd) level referral hospitals the median time for cystectomy was 61.5 days (p = 0.035), in the 2(nd) level referral hospitals - 45 days (p = 0.000) and, in the 1(st) level referral hospitals - 58 days (p = 0.051)., Conclusions: The waiting time from establishing the indications for radical cystectomy to surgery for most cases in Poland does not exceed 90 days.