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945 results on '"Weyand, M."'

161. Crystal structure of the beta Ser178--> Pro mutant of tryptophan synthase. A 'knock-out' allosteric enzyme

Author

Weyand, M, Schlichting, I, Herde, P, Marabotti, Anna, and Mozzarelli, A.

Subjects
Models, Molecular, Binding Sites, Indoles, Proline, Protein Conformation, Glycine, Hydrogen Bonding, Crystallography, X-Ray, Ligands, Protein Structure, Tertiary, Kinetics, Models, Chemical, Glycerophosphates, Mutation, Serine, Tryptophan Synthase, Computer Simulation, Allosteric Site, Protein Binding
Abstract

The catalytic activity of the pyridoxal 5'-phosphate-dependent tryptophan synthase alpha(2)beta(2) complex is allosterically regulated. The hydrogen bond between the helix betaH6 residue betaSer(178) and the loop alphaL6 residue Gly(181) was shown to be critical in ligand-induced intersubunit signaling, with the alpha-beta communication being completely lost in the mutant betaSer(178) --> Pro (Marabotti, A., De Biase, D., Tramonti, A., Bettati, S., and Mozzarelli, A. (2001) J. Biol. Chem. 276, 17747-17753). The structural basis of the impaired allosteric regulation was investigated by determining the crystal structures of the mutant betaSer(178) --> Pro in the absence and presence of the alpha-subunit ligands indole-3-acetylglycine and glycerol 3-phosphate. The mutation causes local and distant conformational changes especially in the beta-subunit. The ligand-free structure exhibits larger differences at the N-terminal part of helix betaH6, whereas the enzyme ligand complexes show differences at the C-terminal side. In contrast to the wild-type enzyme loop alphaL6 remains in an open conformation even in the presence of alpha-ligands. This effects the equilibrium between active and inactive conformations of the alpha-active site, altering k(cat) and K(m), and forms the structural basis for the missing allosteric communication between the alpha- and beta-subunits.

Published
2002

164. Poly(glycerol sebacate)\Poly(butylene succinate‐dilinoleate) Blends as Candidate Materials for Cardiac Tissue Engineering

Author

Tallawi, M., primary, Rai, R., additional, R‐Gleixner, M., additional, Roerick, O., additional, Weyand, M., additional, Roether, J. A., additional, Schubert, D. W., additional, Kozlowska, A., additional, Fray, M. El, additional, Merle, B., additional, Göken, M., additional, Aifantis, K., additional, and Boccaccini, A. R., additional

Published
2013
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188. Reduction of Obliterative Bronchiolitis (OB) by PHD (Prolyl-hydroxylase)-inhibitors activating Hypoxia-inducible transcription factors (HIFs) in an experimental mouse model

Author

Heim, C, primary, Motsch, B, additional, Jalilova, S, additional, Wand, Z, additional, Koch, N, additional, Ramsperger-Gleixner, M, additional, Burzlaff, N, additional, Weyand, M, additional, Bernhardt, W, additional, Eckardt, KU, additional, and Ensminger, S, additional

Published
2013
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192. PHD (Prolyl-hydroxylase)-inhibitor activating Hypoxia-inducible transcription factors (HIFs) reduces levels of transplant arteriosclerosis in a murine aortic allograft model

Author

Heim, C, primary, Jalilova, S, additional, Wang, Z, additional, Motsch, B, additional, Koch, N, additional, Ramsperger-Gleixner, M, additional, Burzlaff, N, additional, Weyand, M, additional, Bernhardt, W, additional, Eckardt, KU, additional, and Ensminger, S, additional

Published
2013
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193. Coronary compliance in patients following orthotopic heart transplantation. An intravascular ultrasound study

Author

Rahmel A, H. H. Scheld, G. Breithardt, Sebastian Kerber, Mario C. Deng, Heinemann-Vechtel O, Weyand M, and F. Günther

Subjects
Adult, Graft Rejection, Male, medicine.medical_specialty, medicine.medical_treatment, Diastole, Sensitivity and Specificity, Reference Values, Internal medicine, Coronary Circulation, Intravascular ultrasound, medicine, Humans, In patient, Systole, Ultrasonography, Interventional, Aged, Heart transplantation, medicine.diagnostic_test, business.industry, Graft Survival, Middle Aged, Coronary Vessels, Compliance (physiology), Transplantation, Coronary vessel, Cardiology, Heart Transplantation, Female, Cardiology and Cardiovascular Medicine, business, Compliance, Follow-Up Studies
Abstract

Background The aim of this study was to investigate coronary compliance in patients early and 71.8 weeks after orthotopic heart transplantation. Methods Thirty patients (mean age 51.4 years, women n=6) underwent coronary angiography early after orthotopic heart transplantation (mean interval 11.6 ± 5.5 weeks), by which time 12 recipients had already been treated for episodes of rejection. A total of 153 different coronary segments were investigated using a mechanical 30 MHz intravascular ultrasound system. In all segments, the intimal index and the circumferential extension of the vessel wall, which had a three-layered appearance, were assessed. Systolic-diastolic changes in area, and pressure with respect to vessel wall area, were used to study normalized compliance. All measurements were repeated in a subgroup of 13 patients 71.8 ± 10.7 weeks after transplantation. Results At the first investigation, the mean intimal index of all estimated cross-sectional areas was 0.07 ± 0.10. The mean circumferential extension of the ‘three-layered’ coronary vessel wall was 74° ± 101°. No correlation could be found between normalized compliance and the intimal index (r= − 0.322, P

Published
1996

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