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Carex laevissima Nakai 1914 (Cyperaceae) is vital for ecological conservation and land virescence, and has high ornamental value. Here the chloroplast genome of Carex laevissima was assembled and systematically analyzed for further genetic research of Carex plants. The chloroplast sequence of Carex laevissima was 188,029 bp in length, including two inverted repeat (IR) regions of 36,699 bp each, a large single-copy (LSC) region of 106,171 bp and a small single-copy region (SSC) of 8460 bp. The overall GC content is 34.0%. It contains 133 genes, including 89 protein-coding, 36 tRNA, and eight rRNA genes. Phylogenetic analysis showed that Carex laevissima is most closely related to Carex neurocarpa.

The structural, magnetocaloric, and magnetic characteristics in Heusler Ni50Mn35In10X5 (X = Ga, Fe, and Al) alloys were examined using X-ray diffraction and field-dependent magnetization measurements. All samples exhibited a mixture structure of cubic L21 and tetragonal L10 and underwent second-order magnetic transitions at TC(Al5) = 220 K, TC(Ga5) = 252 K, and TC(Fe5) = 298 K. The Ga5 alloy exhibited structural change as indicated by a thermal hysteresis that may be seen in the saturation magnetic field in the M(T) dependences. The transition at the TC point from a ferromagnetic to a paramagnetic state caused a drop in magnetization, supported by thermal hysteresis, at a low magnetic field (0.01 T). On the other hand, the Fe5 alloy presented a gradual decrease in magnetization with similar hysteresis behavior, also at a low magnetic field (0.01 T), whereas at 0.1 T of field, no features characteristic of this transition were detected. This could be due to a large difference in the metallic radius of Fe compared to that of In. Otherwise, magnetic investigations demonstrated that the replacement of In with Al may cause the structural transformation temperatures and TC to be shifted to low temperatures. The present results imply that the structural transformation temperatures and the transition itself are highly dependent on chemical composition. Furthermore, under a magnetic field change of 5 T, the maximum magnetic entropy changes of 0.6 J/kg K, 1.4 J/kg K, and 2.71 J/kg K for the Ga5, Fe5, and Al5 alloys, respectively, were determined by their TC. Refrigeration capacity values were found to be 25 J/kg, 74 J/kg, and 98 J/kg at µ0∆H = 5 T. These ribbons are viable candidates for multifunctional applications due to their cheaper cost and their physical characteristics disclosed during the magnetostructural transition, which takes place close to the room temperature.

The phases of high-entropy alloys (HEAs) are closely related to their properties. However, phase prediction bears a significant challenge due to the extensive search space and complex formation mechanisms of HEAs. This study demonstrates a precise and timely methodology for predicting alloy phases. It first developed a machine learning classifier using 145 features and a dataset with 1009 samples to differentiate the four types of alloy phases. Feature selection was performed on the feature set using an Embedded algorithm and a genetic algorithm, resulting in the selection of nine features. The Light GBM algorithm was chosen to train the machine learning model. Finally, the implementation of oversampling and cost-sensitive methods enables LightGBM to tackle the problem of insufficient accuracy in BCC+FCC phase classification. The resulting accuracy of the alloy phase prediction model, evaluated through ten-fold cross-validation, stands at 0.9544.

Magnetic topological materials have a variety of interesting properties, but very few material realizations exist. Here, the authors report a topological nodal-line semimetal and a topological massive Dirac metal phase in EuAs3 and demonstrate a magnetism-driven transition between these phases.

To accurately obtain the true constitutive relationship of each material area in the girth welds of high-strength pipeline steel. Definitely, two kinds of notched round bar specimens were obtained by setting notches on the base metal and in the weld area of X80 steel respectively, and the tensile tests were carried out on the specimens, with the real-time images of specimen notches captured by the high-speed cameras during the tensile process. Then, based on the change law of gray value at the notch edge, an automatic measurement method, i.e. the minimum diameter tracing method, was proposed to measure the minimum sectional diameter of the notch area in real time. In addition, the true constitutive relationship between the base metal and the weld material can be obtained based on the minimum diameter tracing method and the modifier formula, and by fitting the curve obtained with the power law hardening model, the constitutive relationship parameters of corresponding materials can be gained. Finally, the test results were verified with the finite element method. The results show that both of the test method and the results are reliable and effective. The studied test method and the process are of great practical significance for obtaining the constitutive relationship between the base metal and the weld area and realizing the accurate test of the mechanical performance of the pipeline girth welds.

Abstract Vertical ferroelectricity where a net dipole moment appears as a result of in-plane ionic displacements has gained enormous attention following its discovery in transition metal dichalcogenides. Based on first-principles calculations, we report on the evidence of robust vertical ferroelectricity upon interlayer sliding in layered semiconducting β-ZrI2, a sister material of polar semimetals MoTe2 and WTe2. The microscopic origin of ferroelectricity in ZrI2 is attributed to asymmetric shifts of electronic charges within a trilayer, revealing a subtle interplay of rigid sliding displacements and charge redistribution down to ultrathin thicknesses. We further investigate the variety of ferroelectric domain boundaries and predict a stable charged domain wall with a quasi-two-dimensional electron gas and a high built-in electric field that can increase electron mobility and electromechanical response in multifunctional devices. Semiconducting behaviour and a small switching barrier of ZrI2 hold promise for various ferroelectric applications, and our results provide important insights for further development of slidetronics ferroelectricity.

Abstract Background The recurrent aphthous stomatitis (RAS) frequently affects patient quality of life as a result of long lasting and recurrent episodes of burning pain. However, there were temporarily few available effective medical therapies currently. Drug target identification was the first step in drug discovery, was usually finding the best interaction mode between the potential target candidates and probe small molecules. Therefore, elucidating the molecular mechanism of RAS pathogenesis and exploring the potential molecular targets of medical therapies for RAS was of vital importance. Methods Bioinformatics data mining techniques were applied to explore potential novel targets, weighted gene co-expression network analysis (WGCNA) was used to construct a co-expression module of the gene chip data from GSE37265, and the hub genes were identified by the Molecular Complex Detection (MCODE) plugin. Results A total of 16 co-expression modules were identified, and 30 hub genes in the turquoise module were identified. In addition, functional analysis of Hub genes in modules of interest was performed, which indicated that such hub genes were mainly involved in pathways related to immune response, virus infection, epithelial cell, signal transduction. Two clusters (highly interconnected regions) were determined in the network, with score = 17.647 and 10, respectively, cluster 1 and cluster 2 are linked by STAT1 and ICAM1, it is speculated that STAT1 may be a primary gene of RAS. Finally, genistein, daidzein, kaempferol, resveratrol, rosmarinic acid, triptolide, quercetin and (-)-epigallocatechin-3-gallate were selected from the TCMSP database, and both of them is the STAT-1 inhibitor. The results of reverse molecular docking suggest that in addition to triptolide, (-)-Epigallocatechin-3-gallate and resveratrol, the other 5 compounds (flavonoids) with similar structures may bind to the same position of STAT1 protein with different docking score. Conclusions Our study identified STAT1 as the potential biomarkers that might contribute to the diagnosis and potential therapeutic target of RAS, and we can also screen RAS therapeutic drugs from STAT-1 inhibitors.

Abstract The method of object detection has been applied to all aspects in our lives. Although object detection methods based on deep learning have been widely used in various fields, there are still some overlooked problems in the candidate box selection stage. The detection results of traditional candidate box selection methods can only select a relatively optimal maximum candidate box. If the maximum candidate box is still not accurate enough, this type of methods will not be able to do adjust it. To solve this problem, an object detection method based on the multiple candidate box fusion is proposed. The method can not only retain the maximum candidate box and delete the non‐maximum candidate box, but also adjust the position of the maximum candidate box again. Thereby a more accurate maximum candidate box can be obtained. In order to verify the generalization ability of the method, the candidate box fusion method is combined with the two object detection frameworks: faster R‐CNN model and YOLOv3 model. The results of these experiments prove that the proposed method can achieve higher detection accuracy and complete the object detection task more effectively.

Simulation of changes in ecosystem service value (ESV) caused by land use change in Wuhan under multiple scenarios is of great significance for ensuring urban ecological security and enhancing regional ecosystem service values. The city of Wuhan was selected as the study area, and the changes in land use and ESV in Wuhan over the past 31 years were analyzed and calculated based on five-phase remote sensing images and statistical yearbooks for 1990, 1998, 2006, 2014 and 2021. On this basis, the CA-Markov model and the multi objective planning (MOP) model were used to simulate the land use change in the study area in 2040 under four scenarios (natural development scenario, cultivated land protection scenario, ecological protection scenario and urban development scenario), and the total ESV was estimated under each scenario. The total value of ecosystem services was estimated under each scenario, and grid tools were applied to visualize the spatial distribution and degree of aggregation of ecosystem services. The results show that: (1) The most obvious feature of land use change in Wuhan from 1990 to 2021 is the sharp reduction in arable land area and the rapid expansion of build-up land area. Over the past 31 years, the arable land area decreased by 78322.4 hm2, and the build-up land area increased by 52559.28 hm2. (2) From 1990 to 2021, Wuhan’s total ESV at the five timepoints (1990, 1998, 2006, 2014, 2021) was 74.554 billion yuan, 71.512 billion yuan, 69.632 billion yuan, 73.433 billion yuan and 68.548 billion yuan, respectively. Overall, there has been a downward trend in volatility. (3) Under the four scenarios, the ESV in 2040 is projected to be 72.777 billion yuan, 70.969 billion yuan, 74.097 billion yuan or 70.620 billion yuan, respectively. Among them, the ecological protection scenario is the optimal simulation choice. (4) The cold and hot spots of ESV show an aggregated distribution over a large area, with the hot spots mainly concentrated in the central and southeastern parts of Wuhan and the cold spots mainly located in the northeastern and northwestern portions of Wuhan. Simulating the future land use change trends in Wuhan and exploring the responses of ecosystem service values under various scenarios are conducive to the construction of a new pattern of urban land space development and protection and can provide a scientific basis and a reference for decision-making for comprehensively promoting the sustainable development of Wuhan and other metropolitan areas in China in the future.

Zhilong Huoxue Tongyu capsule (ZHTC) is an effective traditional Chinese medicine compound for the treatment of ischemic stroke, which is widely used in clinical ischemic stroke patients. However, it is uncertain whether ZHTC affects ischemic stroke through gut microbiota and serum metabolites. In this study, a rat model of middle cerebral artery occlusion (MCAO) was prepared. By evaluating motor nerve function score, cerebral infarct size, brain tissue damage and intestinal barrier damage, it was found that ZHTC improved stroke-related symptoms in MCAO rats. Using 16S rRNA gene sequencing, fecal microbial transplantation (FMT), untargeted metabolomics, and spearman correlation analysis of gut microbiota and serum metabolites, we found that ZHTC can regulate the abundance of p_Firmicutes, p_Bacteroidota,p_Proteobacteria, g_Prevotella, and g_Lactobacillus, and regulated 23 differential metabolites. Spearman correlation analysis found that Arginine was positively correlated with p_Firmicutes, o_Clostridiales, c_Clostridia, and negatively correlated with p_Bacteroidetes, c_Bacteroidia,o_Bacteroidales; L-Lysine was negatively correlated with f_Christensenellaceae; L-methionine was positively correlated with o_Lactobacillales, f_Lactobacillaceae, and g_Lactobacillus. Altogether, this study shows for the first time that ZHTC can ameliorate ischemic stroke by modulating gut microbiota and metabolic disturbances. This lays the foundation for further revealing the causal relationship between ZHTC, gut dysbiosis, plasma metabolite levels and ischemic stroke, and provides a scientific explanation for the ameliorating effect of ZHTC on ischemic stroke.

The number of thoracolumbar vertebrae in Dezhou donkeys varies from 22 to 24 and is associated with body size and carcass traits. In mammals, the latent transforming growth factor beta binding protein 2 (LTBP2) has been found to have some functions in the development of thoracolumbar vertebrae. The relationship between LTBP2 and TLN (the number of thoracolumbar vertebrae) of Dezhou donkeys is yet to be reported. The purposes of this study are as follows: 1) to quantify the effect of thoracolumbar vertebrae number variation of Dezhou donkeys on body size and carcass trait; 2) to study the distribution of single nucleotide variants (SNVs) in the LTBP2 gene of Dezhou donkeys; and 3) to explore whether these SNVs can be used as candidate sites to study the mechanism of Dezhou donkey muti-thoracolumbar vertebrae development. The TLN, body size, and carcass traits of 392 individuals from a Dezhou donkey breed were recorded. All animals were sequenced for LTBP2 using GBTS liquid chip and 16 SNVs were used for further analysis. We then analyzed the relationship between these SNVs with TLN, body size, and carcass traits. The results showed that: 1) c.5547 + 860 C > T, c.5251 + 281 A > C, c.3769 + 40 C > T, and c.2782 + 3975 A > G were complete genetic linkages and significantly associated with thoracic vertebrae number (TN) (p < 0.05) (wild-type homozygotes had more TN than heterozygotes); 2) c.1381 + 768 T > G and c.1381 + 763 G > T were significantly associated with lumber vertebrae number (LN) (p < 0.05); 3) c.1003 + 704 C > T, c.1003 + 651 C > T, c.1003 + 626 A > G, and c.812 + 22526 T > G were significantly associated with chest circumference (CHC), front carcass weight (CWF), after carcass weight (CWA), and carcass weight (CW) (p < 0.05) (wild-type homozygotes were larger than other genotypes in CHC, CWF, CWA, and CW); and 4) the effect of variation is not consistent in c.565 + 11921 A > G, c.565 + 6840 A > G, c.565 + 3453 C > T, and c.494 + 5808 C > T. These results provide useful information that the polymorphism of LTBP2 is significantly associated with TLN, body size, and carcass traits in Dezhou donkeys, which can serve as a molecule marker to improve donkey production performance.

ObjectiveThe aim of the study is to develop and validate a deep learning model to predict the platinum sensitivity of patients with epithelial ovarian cancer (EOC) based on contrast-enhanced magnetic resonance imaging (MRI).MethodsIn this retrospective study, 93 patients with EOC who received platinum-based chemotherapy (≥4 cycles) and debulking surgery at the Sun Yat-sen Memorial Hospital from January 2011 to January 2020 were enrolled and randomly assigned to the training and validation cohorts (2:1). Two different models were built based on either the primary tumor or whole volume of the abdomen as the volume of interest (VOI) within the same cohorts, and then a pre-trained convolutional neural network Med3D (Resnet 10 version) was transferred to automatically extract 1,024 features from two MRI sequences (CE-T1WI and T2WI) of each patient to predict platinum sensitivity. The performance of the two models was compared.ResultsA total of 93 women (mean age, 50.5 years ± 10.5 [standard deviation]) were evaluated (62 in the training cohort and 31 in the validation cohort). The AUCs of the whole abdomen model were 0.97 and 0.98 for the training and validation cohorts, respectively, which was better than the primary tumor model (AUCs of 0.88 and 0.81 in the training and validation cohorts, respectively). In k-fold cross-validation and stratified analysis, the whole abdomen model maintained a stable performance, and the decision function value generated by the model was a prognostic indicator that successfully discriminates high- and low-risk recurrence patients.ConclusionThe non-manually segmented whole-abdomen deep learning model based on MRI exhibited satisfactory predictive performance for platinum sensitivity and may assist gynecologists in making optimal treatment decisions.

The biofloc technology (BFT) system has been widely applied in the shrimp and fish culture industry for its advantages in water-saving, growth improvement, and water quality purification. However, The BFT system usually takes a long time to establish, and the extra carbon source input increases the maintenance cost of the system. In this study, we aimed to develop a low-cost and high-efficient BFT system for Litopenaeus vannamei by applying bacteria that could promote the formation of BFT and utilize cheap carbon sources. Three bioflocculant-producing bacteria strains (M13, M15, and M17) have been screened from a cellulolytic strain collection. All three strains have been identified as Bacillus spp. and can use sugarcane bagasse (SB) as a carbon source, which is a cheap byproduct of the sucrose industry in the tropic area of China. Compared to sucrose, the addition of SB and the three strains could improve the biofloc formation rate, biofloc size distribution, ammonia removal rate, and the growth performance of the shrimps. These results suggest that the bioflocculant and cellulase-producing bacteria strains could promote the biofloc formation and the growth of shrimps by using SB as an economic substitute carbon source in the BFT shrimp culture system.

Four lactic acid bacteria with a high capacity for acid production were screened from 113 lactic acid bacteria (LAB) isolated from aquaculture systems, and were identified as Lactococcus lactis (S1 and S2), Enterococcus faecali (F3 and F7) by 16S rDNA sequencing. These four candidates exhibited favorable probiotic properties, including no haemolytic activity, good auto-aggregation performance, tolerance to simulated gastric fluid, and antagonistic activity against pathogens. To determine the ideal probiotic feeding dose and duration that would maximize protection against pathogens, L. vannamei were fed a probiotic supplemented diet at a series of concentrations for up to 60 days, and then challenged with Vibrio harveyi every 15 days. In vivo studies demonstrated that supplementation with L. lactis (S1 and S2) and E. faecalis (F3 and F7) significantly improved growth performance and feed utilization in the probiotic-treated groups compared to the control group. The findings suggested that administration of F7 and S2 strains at 106 CFU g−1 for 30–45 d was more effective at suppressing V. harveyi infection than any of the other feeding regimens. This study establishes a theoretical foundation for the practical use of probiotics.

The measurement of nitric oxide (NO) concentration in power plant flue gas by tunable diode laser absorption spectroscopy (TDLAS) is susceptible to fly ash particles. The presence of fly ash particles causes laser intensity fluctuations and leads to the second harmonic (2f) signal amplitude changes. In this manuscript, we analyzed the influence of particles on the 2f signal and corrected the measurement error of NO concentration by the wavelength modulation-second harmonic (WMS-2f) technique. Specifically, the 2f signal error caused by quartz sand and fly ash particles were measured, and the exponential function representing the peak-to-peak error of the 2f signal was obtained by fitting. Finally, the peak-to-peak value of the 2f signal and the NO concentration were corrected according to the exponential function. After correction, the NO concentration error under the fly ash influence (the particle size in the range of 10–200 μm and the mass concentration is less than 18.26 g/m3) was no more than 2%. This correction method effectively improves the measurement accuracy of NO concentration by the WMS-2f technique in the fly ash environment.

The planting of Cyperus esculentus, a member of the grass family Cyperaceae which includes nut sedge weeds, is being increasingly promoted in northern China’s semi-arid and arid regions. Yet the effects of planting C. esculentus upon soil quality and soil microbial characteristics of sandy land remain unclear. This study examined the short-term (1 year) impact of this grass species on soil microbial biomass indices, enzymatic activities, and microbiome characteristics in the Horqin Sandy Land area of China. The results show that planting C. esculentus could increase microbial biomass in the form of carbon (MBC), nitrogen (MBN), and phosphorus (MBP), but it negligibly influenced the enzymatic activities of soil β-1,4-glucosidase (BG), cellobiohydrolase (CBH), leucine aminopeptidase (LAP), and β-1,4-N-acetaminoglycosidase (NAG). Over 1 year, we found that planting C. esculentus significantly increased the soil bacterial richness and diversity of sandy land, yet also altered community composition of soil bacteria and eukaryotes in way that could promote their homogenization. In this respect, the relative abundances of Acidobacteria and Proteobacteria significantly decreased and increased, respectively; hence, they may be considered for use as important indicators of soil nutrient-rich conditions. Overall, the results could be explained by greater soil organic carbon (SOC) and total nitrogen (TN), mainly derived from cumulative plant litter input to soils, which then increased the sandy soil’s C:N ratio. Future research should focus on exploring the long-term effects of planting C. esculentus on soil quality and soil microbial characteristics of sandy lands in China and abroad.

ObjectiveTo evaluate HIF2α polymorphisms and glucose metabolism in a group of women with polycystic ovary syndrome (PCOS) or unexplained infertility (UI).PatientsThe infertile group (n=148) consisted of 96 women with PCOS, 52 women with UI, and176 women without infertility as a healthy control group.InterventionWe genotyped 29 single nucleotide polymorphisms (SNPs) of HIF2α by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based genotyping technology. The genetic associations were analyzed statistically.Main outcome measuresAllele frequency, genotype distribution and haplotype analyze of the HIF2α polymorphisms were performed. Body mass index (BMI), waist circumference, uric acid (UA), high-sensitivity C-reactive protein (hsCRP), lipids, glucose and insulin tolerance - were also measured.ResultsInfertile women with PCOS had a higherBMI and waist circumference, elevated hsCRP and uric acid (UA) levels, impaired glucose tolerance, and increased levels of plasma insulin compared to UI patients and healthy women. SNP analysis of HIF2α revealed that the allele and genotype frequencies of rs4953361 were significantly associated with infertile women with PCOS. Haplotype analysis of the HIF2α polymorphism identified haplotypes TGG and TGA as being associated with infertile women with PCOS. Women with the AA genotype of rs4953361 had a significantly higher BMI and post load plasma glucose and insulin levels than those of women with the GG genotype.ConclusionInfertile women with PCOS more commonly have metabolic disturbances than those with UI. This is the first study to report an association between HIF2α polymorphisms (rs4953361) and the risk of infertile women with PCOS, not UI, in Han Chinese population. These results require replication in larger populations.In this observational study, we did not report the results of a health care intervention on human participants. The study was approved by the Human Research Ethics Committee of the First Affiliated Hospital of Chongqing Medical University. Clinical data and peripheral blood samples were collected only after explaining the objectives of the study and obtaining a signed informed consent form.

PurposeTo investigate the effect of sub-satisfactory stent recanalization on hemodynamic stresses for severe stenoses of the middle cerebral artery (MCA) M 1 segment.Materials and methodsPatients with severe stenoses of the MCA M1 segment treated with endovascular stent angioplasty were retrospectively enrolled. Three-dimensional digital subtraction angiography before and after stenting was performed; the computational fluid dynamics (CFD) analysis of hemodynamic stresses at the stenosis and normal segments proximal and distal to the stenoses was analyzed.ResultsFifty-one patients with severe stenosis at the MCA M1 segment were enrolled, with the stenosis length ranging from 5.1 to 12.8 mm (mean 9 ± 3.3 mm). Stent angioplasty was successful in all (100%) the patients. The angiography immediately after stenting demonstrated a significant (P < 0.05) decrease in MCA stenosis after comparison with before stenting (31.4 ±12.5% vs. 87.5 ± 9.6%), with residual stenosis of 15–30% (mean 22.4 ± 3.5%). Before stenting, the total pressure was significantly higher (P < 0.0001), while the WSS, velocity, and vorticity were all significantly decreased (P < 0.0001) at the normal arterial segment proximal to the stenosis, and the total pressure, WSS, velocity, and vorticity were all significantly decreased (P < 0.0001) at the normal arterial segment distal to the stenosis compared with those at the stenosis. After sub-satisfactory stenting recanalization, all the hemodynamic stresses proximal or distal to the stenosis and at the perforator root were improved compared with those before stenting and were similar to those after virtual stenosis removal.ConclusionSub-satisfactory recanalization of severe MCA stenoses can significantly improve the hemodynamic status for cerebral perfusion at the stenoses.

Abstract As a monocotyledonous plant in family Cyperaceae, yellow nutsedge (Cyperus esculentus L.) is unique in accumulating a substantial amount of oil in underground tubers and provides a model system for studying oil accumulation in nonseed tissues. However, no data on the mitochondrial and nuclear genome sequences of this species are available, which greatly limits our understanding of its evolutionary characteristics and some essential biological mechanisms. In the present study, we report the first complete mitochondrial genome sequence of the cultivated yellow nutsedge. The analysis of the genome showed that the yellow nutsedge mitochondrial genome is 1,002,696 bp in size and encodes 62 genes consisting of 36 protein‐coding genes (PCGs), 20 transfer RNA (tRNA) genes, and six ribosomal RNA (rRNA) genes. Compared with other angiosperms, yellow nutsedge mitochondrial genome contains much higher percentage of noncoding sequences (95.36%). Sixteen plastid‐derived fragments were identified to be strongly associated with mitochondrial genes including one intact plastid‐related gene (ndhH). Comparative analysis with seven other sequenced plant mitochondrial genomes revealed that two syntenic gene clusters, rps3‐rpl16 and rps12‐nad3, are highly conserved in all plant mitochondrial genomes, and the mitochondrial genome of yellow nutsedge is more similar to those of monocotyledons in the gene order. Phylogenetic analysis based on 13 shared protein‐encoding genes in eight plant species showed that yellow nutsedge is evolutionarily more closely related to monocotyledonary species. Overall, the species‐specific features of the cultivated yellow nutsedge mitochondrial genome provide additional information for the evolutionary and comparative genomic studies in the yellow nutsedge and other Cyperus species of the Cyperaceae family.

Photoacoustic spectroscopy (PAS) based gas sensors with high sensitivity, wide dynamic range, low cost, and small footprint are desirable in energy, environment, safety, and public health. However, most works have focused on either acoustic resonator to enhance acoustic wave or optical resonator to enhance optical wave. Herein, we develop a gas sensor based on doubly resonant PAS in which the acoustic and optical waves are simultaneously enhanced using combined optical and acoustic resonators in a centimeter-long configuration. Not only the lower detection limit is enhanced by the double standing waves, but also the upper detection limit is expanded due to the short resonators. As an example, we developed a sensor by detecting acetylene (C2H2), achieving a noise equivalent absorption of 5.7 × 10−13 cm−1 and a dynamic range of eight orders. Compared to the state-of-the-art PAS gas sensors, the developed sensor achieves a record sensitivity and dynamic range.

Background: Aortic dissection (AD) is a macrovascular disease which is pathologically characterized by aortic media degeneration (AMD). Our team's previous research found that iron deficiency (ID) promoted the formation of AMD through presentative research. In this study, we aimed to investigate the underlying mechanism of ID promoting AMD formation. Methods: The human aortic tissues were harvested from AD patients and organ donors. ApoE-/- mice were simultaneously given AngII infusion and low-iron feed to investigate the relationship between ID and AD. The IRE1-XBP1-CHOP signal axis of endoplasmic reticulum (ER) stress was selectively inhibited with 4μ8C. Iron contents were detected by Perls staining. The expression of iron metabolism and ER stress-relative proteins were analyzed by IF and western blotting. Apoptosis rates of aortic tissue and ASMCs were detected by TUNEL staining and flow cytometry, and ROS content was also measured by the flow cytometry. Results: ID was accompanied by ER stress in patients with AD. Among the three signaling pathways of ER stress in ID-induced AMD, proteins of IRE1, PERK and ATF6 signaling pathways were up-regulated by 2.65 times, 1.14 times and 1.24 times, respectively. ID was positively related to ER stress, mitochondrial oxidative stress and aortic media apoptosis in vivo and in vitro assays, while 4μ8C reversed the severity of ER stress and AMD. Conclusions: ID could activate ER stress by eliciting mitochondrial oxidative stress to activate the IRE1-XBP1-CHOP signaling pathway in the ER, which accelerated the apoptosis of ASMCs in aortic media, thus promoting the formation of AMD.

This paper reports on a study that examines how a school teacher, a university researcher, and a professional textbook writer interact when they collaborate in designing materials for English language teaching. Data were collected from interviews with the participants and audio recordings of their interaction in collaborative design supplemented with drafts of the designed materials. The findings showed that the three participants placed different emphases on the design of the teaching materials due to their individual principles, values, and approaches towards English language learning and teaching and materials design. Their values and beliefs were found to inform their practices in collaborative materials design and thus influenced the processes of interaction and negotiation. The study contributes to our understanding of the personalized and diversified principles and beliefs of the stakeholders regarding the development of teaching materials, and suggests that textbook writers and their publishers need to pay more heed to the parties for whom they are producing their materials.

Nitrogen (N) deficiency is one of the main factors limiting maize (Zea mays L.) productivity. Genetic improvement of root traits could improve nitrogen use efficiency. An association panel of 461 maize inbred lines was assayed for root growth at seedling emergence under high-nitrate (HN, 5 mmol L−1) and low-nitrate (LN, 0.05 mmol L−1) conditions. Twenty-one root traits and three shoot traits were measured. Under LN conditions, the root-to-shoot ratio, root dry weight, total root length, axial root length, and lateral root length on the primary root were all increased. Under LN conditions, the heritability of the plant traits ranged from 0.43 to 0.82, a range much wider than that of 0.27 to 0.55 observed under HN conditions. The panel was genotyped with 542,796 high-density single-nucleotide polymorphism (SNP) markers. Totally 328 significant SNP markers were identified using either mixed linear model (MLM) or general linear model analysis, with 34 detected by both methods. In the 100-kb intervals flanking these SNP markers, four candidate genes were identified. Under LN conditions, the protoporphyrinogen IX oxidase 2 gene was associated with total root surface area and the DELLA protein-encoding gene was associated with the length of the visible lateral root zone of the primary root. Under HN conditions, a histone deacetylase gene was associated with plant height. Under both LN and HN conditions, the gene encoding MA3 domain-containing protein was associated with the first whorl crown root number. The phenotypic and genetic information from this study may be exploited for genetic improvement of root traits aimed at increasing NUE in maize.

Abstract Background Wolfram syndrome (WFS) is a rare autosomal recessive genetic disease whose main cause is mutations in the WFS1 and CISD2 genes. Its characteristic clinical manifestations are diabetes insipidus, diabetes mellitus, optic atrophy and deafness. Methods In this study, two patients from this particular family underwent complete routine biochemical and ophthalmic tests. Blood, urine, routine stool test, visual acuity (VA) examination, visual field assessment, funduscope, optical coherence tomography and periorbital magnetic resonance imaging (MRI) scans were performed for each patient to evaluate whether the nerve fiber layer around the optic nerve head was atrophied and next-generation sequencing of target genes was performed in two patients. Results When the patients were diagnosed with Wolfram syndrome, their genetic analyses suggested unique three-site compound heterozygous mutations (c.2314C > T + c.2194C > T + c.2171C > T) in exon 8 of both patients’ chromosome 4. One mutation (c.2314C > T) was a novel mutation in the known reports of Wolfram syndrome. As a degenerative genetic disease, the types of gene mutations in the Chinese population are generally homozygous mutations at the unit point or compound heterozygous mutations at two nucleotide change sites. However, the two patients reported in this study are the first known cases of compound heterozygous mutations with three mutation sites coexisting on the WFS1 gene in China or even globally. Conclusions This study expands the phenotypic spectrum of Wolfram syndrome and may reveal a novel mutation pattern of pathogenesis of Wolfram syndrome. The implications of this discovery are valuable in the clinical diagnosis, prognosis, and treatment of patients with WFS1.

Objective This study explored the mechanism of the Kelch-like erythroid cell-derived protein with CNC homology-associated protein 1 (Keap1)–nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway under conditions of zearalenone (ZEA)-induced oxidative stress in the duodenum of post-weaning gilts. Methods Forty post-weaning gilts were randomly allocated to four groups and fed diets supplemented with 0, 0.5, 1.0, or 1.5 mg/kg ZEA. Results The results showed significant reductions in the activity of the antioxidant enzymes total superoxide dismutase and glutathione peroxidase and increases the malondialdehyde content with increasing concentrations of dietary ZEA. Immunohistochemical analysis supported these findings by showing a significantly increased expression of Nrf2 and glutathione peroxidase 1 (GPX1) with increasing concentrations of ZEA. The relative mRNA and protein expression of Nrf2, GPX1 increased linearly (p

Abstract Life is indeed continuously going through the irreversible and inevitable process of aging. The rate of aging process depends on various factors and varies individually. These factors include various environmental stimuli including exposure to toxic chemicals, psychological stress whereas suffering with various illnesses specially the chronic diseases serve as endogenous triggers. The basic underlying mechanism for all kinds of stresses is now known to be manifested as production of excessive ROS, exhaustion of ROS neutralizing antioxidant enzymes and proteins leading to imbalance in oxidation and antioxidant processes with subsequent oxidative stress induced inflammation affecting the cells, tissues, organs and the whole body. All these factors lead to conventional cell death either through necrosis, apoptosis, or autophagy. Currently, a newly identified mechanism of iron dependent regulated cell death called ferroptosis, is of special interest for its implication in pathogenesis of various diseases such as cardiovascular disease, neurological disorders, cancers, and various other age-related disorders (ARD). In ferroptosis, the cell death occur neither by conventional apoptosis, necrosis nor by autophagy, rather dysregulated iron in the cell mediates excessive lipid peroxidation of accumulated lethal lipids. It is not surprising to assume its role in aging as previous research have identified some solid cues on the subject. In this review, we will highlight the factual evidences to support the possible role and implication of ferroptosis in aging in order to declare the need to identify and explore the interventions to prevent excessive ferroptosis leading to accelerated aging and associated liabilities of aging.

Extreme rainfall is the main contributing factor to landslides. Therefore, it is of great significance to monitor and forecast short-term rainfall in landslide-prone areas. However, the spatial scale of landslide-prone areas is small, and traditional numerical forecast models have difficulty in accurately forecasting rainfall on this scale. To solve the above problem, this study proposes a short-term rainfall forecasting method for landslide-prone areas by combining the back-propagation neural network (BP-NN) algorithm and global navigation satellite system (GNSS) observations to achieve accurate short-term rainfall forecasting in landslide-prone areas. Firstly, a high-precision atmospheric weighted-average temperature (Tm) model is established using radiosonde data to obtain high-precision precipitable water vapor (PWV) estimates. Secondly, the BP-NN algorithm is introduced, and the GNSS-derived PWV, temperature and pressure from a meteorological station, and rainfall for the previous and next hour are used as input parameters to establish a BP-NN-based rainfall forecast model. As an illustrative case, experiments are conducted in a landslide-prone area in Yunnan Province using data from 15 GNSS stations and the corresponding meteorological station. Statistical results show that the established regional Tm model has high accuracy, with an average root mean square (RMS) and bias of 3 K and 0.15 K, respectively. In addition, the short-term rainfall forecast model based on the BP algorithm achieves a true detection rate of up to 93.70% and a false forecast rate of as low as 38.30%, which is significant for short-term rainfall forecasting in landslide-prone areas.

Soil phosphorus (P) limitation in karst areas has severely constrained soil quality and land productivity. To enhance silage maize yield and quality and alleviate and/or balance the low phosphorus availability in the karst areas of China, the experiment investigated the effects of different tillage and residue retention practices on silage maize yield and quality and soil phosphorus in this region. The treatment set included: conventional tillage (CT), conventional tillage and root stubble retention (CTH), conventional tillage and mulch (CTM), conventional tillage and crushing and incorporation of hairy vetch by tillage (CTR), no tillage (NT), no tillage and root stubble retention (NTH), no tillage and mulch (NTM), and no tillage and living mulch (NTLM). The results showed that CTM, NTM, CTR, and NTLM significantly increased the height and LAI of silage maize compared with the CT, NT, and NTH treatments. CTM, CTR, and NTM significantly enhanced maize yield. Compared with conventional tillage, not tilling had a more pronounced improvement in silage quality, whereas residue retention hardly affected corn quality. In addition, although not tilling does not significantly increase acid phosphatase activity, it appeared to be advantageous in increasing soil microbial phosphorus and available phosphorus content when combined with cover crop measures. Ultimately, we concluded that NTM and NTLM are beneficial for silage maize yield and quality and soil phosphorus content in karst areas and verified the advantages of combining no tillage and residue retention practices for silage maize production and soil phosphorus improvement in the karst areas of China.

This paper proposed a Pulse-Agile-Phase-Coding slow-time MIMO (PAPC-st-MIMO) waveform, where the phase-coded signal is utilized as the intra-pulse modulation of the slow-time MIMO waveform. Firstly, the signal model of the proposed waveform is derived. To improve the orthogonality of the phase-coded waveform sets, a novel hybrid evolutionary algorithm based on Cyclic Algorithm New (CAN) is proposed. After the optimization process of the phase-coded waveform sets, the signal processing method of the PAPC-st-MIMO waveform is derived. Finally, the effectiveness of the proposed method is verified with a simulation experiment. The mitigation ratio of the near-range detection waveform can achieve −30 dB, while the long-range detection waveform can achieve −35 dB. This approach ensures waveform orthogonality while enabling the slow-time MIMO waveform to achieve distance selectivity. By conducting joint pulse-Doppler processing across multiple range segments, range ambiguity can be suppressed, increasing the system’s Pulse Repetition Frequency (PRF) without introducing ambiguity.

Effective in-season crop nitrogen (N) status diagnosis is important for precision crop N management, and remote sensing using an unmanned aerial vehicle (UAV) is one efficient means of conducting crop N nutrient diagnosis. Here, field experiments were conducted with six N levels and six maize hybrids to determine the nitrogen nutrition index (NNI) and yield, and to diagnose the N status of the hybrids combined with multi-spectral data. The NNI threshold values varied with hybrids and years, ranging from 0.99 to 1.17 in 2018 and 0.60 to 0.71 in 2019. A proper agronomic optimal N rate (AONR) was constructed and confirmed based on the measured NNI and yield. The NNI (R2 = 0.64–0.79) and grain yield (R2 = 0.70–0.73) were predicted well across hybrids using a random forest model with spectral, structural, and textural data (UAV). The AONRs calculated using the predicted NNI and yield were significantly correlated with the measured NNI (R2 = 0.70 and 0.71 in 2018 and 2019, respectively) and yield (R2 = 0.68 and 0.54 in 2018 and 2019, respectively). It is concluded that data fusion can improve in-season N status diagnosis for different maize hybrids compared to using only spectral data.

The lipid molecules and volatile organic compounds (VOCs) in milk are heavily influenced by diet. However, little is known about how roughage affects the lipid and VOC contents of donkey milk. Accordingly, in the present study, donkeys were fed corn straw (G1 group), wheat hulls (G2 group), or wheat straw (G3 group), and the lipid and VOC profiles of their milk were determined using LC–MS and GC–MS. Of the 1842 lipids identified in donkey milk, 153 were found to be differential, including glycerolipids, glycerophospholipids, and sphingolipids. The G1 group showed a greater variety and content of triacyclglycerol species than the G2 and G3 groups. Of 45 VOCs, 31 were identified as differential, including nitrogen compounds, esters, and alcohols. These VOCs were significantly increased in the G2 and G3 groups, with the greatest difference being between the G1 and G2 groups. Thus, our study demonstrates that dietary roughage changes the lipid and VOC profiles of donkey milk.

Digital construction relies on effective sensing to enhance the safety, productivity, and quality of its activities. However, current sensing devices (e.g., camera, LiDAR, infrared sensors) have significant limitations in different aspects. In light of the substantial advantages offered by emerging 4D mmw technology, it is believed that this technology can overcome these limitations and serve as an excellent complement to current construction sensing methods due to its robust imaging capabilities, spatial sensing abilities, velocity measurement accuracy, penetrability features, and weather resistance properties. To support this argument, a scientometric review of 4D mmw-based sensing is conducted in this study. A total of 213 articles published after the initial invention of 4D mmw technology in 2019 were retrieved from the Scopus database, and six kinds of metadata were extracted from them, including the title, abstract, keywords, author(s), publisher, and year. Since some papers lack keywords, the GPT-4 model was used to extract them from the titles and abstracts of these publications. The preprocessed metadata were then integrated using Python and fed into the Citespace 6.2.R3 for further statistical, clustering, and co-occurrence analyses. The result revealed that the primary applications of 4D mmw are autonomous driving, human activity recognition, and robotics. Subsequently, the potential applications of this technology in the construction industry are explored, including construction site monitoring, environment understanding, and worker health monitoring. Finally, the challenges of adopting this emerging technology in the construction industry are also discussed.

Abstract Using a modified Landau-Devonshire type thermodynamic potential, we show that dielectric tunability η of a tetragonal ferroelectric film can be analytically solved. At a given electric field E, η is a function of the remnant polarization ( $$P_0^f$$ P 0 f ) and the small-field relative dielectric permittivity ( $$\chi _0^f$$ χ 0 f ), which are commonly measured material properties. After a survey of materials, a large η~80% is predicted to be achievable in a (001)-oriented tetragonal (K0.5,Na0.5)NbO3 film. This strain-stabilized tetragonal phase is verified by density functional theory (DFT) calculations. (K0.5,Na0.5)NbO3 films based on this design were successfully prepared via a sputtering deposition process on SrRuO3-buffered (100)SrTiO3 substrates. The resulted epitaxial films showed a sizable $$P_0^f$$ P 0 f (~0.21C m−2) and a large $$\chi _0^f$$ χ 0 f (~830–860), as well as a large η close to the theoretical value. The measured dielectric tunabilities as functions of E are well described by the theoretical η(E) curves, validating our integrated approach rooted in a theoretical understanding.

Ultrastrong light-matter interactions with dominant antiresonant terms are expected to give rise to interesting phenomena such as quantum fluctuation suppression. Here, the authors propose a system of ultrastrongly coupled magnon modes in a rare earth orthoferrite as a platform for exploring such phenomena.

Abstract Background The Reporting Items for Practice Guidelines in Healthcare (RIGHT) checklist was used to assess the reporting quality of 2009–2019 clinical practice guidelines (CPGs) regarding gout and hyperuricemia, aimed to improve the reporting quality of future guidelines. Methods We searched PubMed, the Chinese Biomedical Literature database, the Wanfang Database, and the China National Knowledge Infrastructure from January 2009 to June 2019 for guidelines regarding gout and hyperuricemia. We also searched the websites of guideline development organizations (the Guidelines International Network, the National Institute for Health and Clinical Excellence, the American College of Rheumatology, and the European League Against Rheumatism (EULAR)). Furthermore, supplementary guidelines reported in included articles were systematically searched, as well as Google Scholar. Results Seventeen guidelines were included, of which one was in Chinese and 16 were in English. The mean reporting rate of the 35 items specified was 14.9 (42.5%); only five CPGs (29.4%) had a reporting rate >50%. Of the 35 items, three were very frequently reported. The reporting proportion of the seven domains (basic information, background, evidence, recommendations, review and quality assurance, funding and declaration and management of interests, and other information) were 64.7%, 36.8%, 50.6%, 42.9%, 8.82%, 33.8%, and 31.4%, respectively. Conclusion The reporting quality of the present guidelines for gout and hyperuricemia is relatively poor. We suggest that the RIGHT reporting checklist should be used by CPG developers to ensure higher reporting quality of future guidelines.

Abstract Background Minqin is suffering from a serious desertification, whereas the knowledge about its bacterial community is limited. Herein, based on Nitraria tangutorum and Haloxylon ammodendron from Minqin, the bacterial community diversities in fixed sandy land, semi-fixed sandy land and shifting sandy land were investigated by combining with culture-dependent and culture-independent methods. Results Minqin stressed with high salinity and poor nutrition is an oligotrophic environment. Bacterial community in Minqin was shaped primarily by the presence of host plants, whereas the type of plant and sandy land had no marked effect on those, which displayed a better survival in the rhizospheres of N. tangutorum and H. ammodendron. The dominant groups at phyla level were Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, Acidobacteria and Candidate_division_TM7. The abundance of Firmicutes with ability of desiccation-tolerance was significantly higher in harsh environment, whereas Bacteroidetes were mainly distributed in areas with high nutrient content. The abundances of Proteobacteria and Bacteroidetes were relatively high in the rhizospheres of N. tangutorum and H. ammodendron, which had more plant-growth promoting rhizobacteria. A large number of Actinobacteria were detected, of which the most abundant genus was Streptomyces. The physicochemical factors related to the diversity and distribution of the bacterial community were comprehensively analyzed, such as pH, electrical conductivity, soil organic matter, C/N and sand, and the results indicated that Minqin was more suitable for the growth of N. tangutorum, which should be one of most important sand-fixing plants in Minqin. Conclusions The bacterial community diversities in different types of sandy lands of Minqin were comprehensively and systematically investigated by culture-dependent and culture-independent approaches, which has a great significance in maintaining/restoring biological diversity.

Summary: Tail wagging plays an important role in social interactions, e.g., dogs show asymmetrical tail wagging in response to different social stimuli. However, the effects of social cues on tail wagging and the intrinsic organization of wagging behavior remain largely unknown. Here, we developed a platform using a deep-learning-based motion-tracking technique to extract and analyze the movement trajectory of a dog’s tail tip during dog-human interactions. Individual dogs exhibited unique and stable wagging characteristics. We further found that tail wagging developed asymmetry toward the right side over three days of dog-human interactions, suggesting that it is a time-sensitive indicator of social familiarity. In addition, wagging appeared to follow an attractor-like dynamic process consisting of stable states and unstable, transitional states. Together, these results revealed sophisticated characteristics and organization of a dog’s tail-wagging behavior during interactions with humans, providing a useful paradigm for studying dogs’ social behaviors and the underlying neural mechanisms.

Primary malignant melanoma of the esophagus (PMME) is a rare gastrointestinal melanoma with a high rate of recurrence and metastasis. The standard of care for PMME has not been established yet due to a lack of understanding of its clinical and molecular pathogenesis. Thus, we performed genomic profiling on a recurrent PMME case to seek novel opportunities for the management of this rare disease. Between 2013 and 2016, 6 tissue samples including 3 from the primary tumors, 2 from the relapsed tumors, and 1 from a normal control were collected from a patient diagnosed with PMME and were subjected to whole-exome sequencing to track the dynamic genetic changes. Additionally, we also analyzed a cohort of 398 samples obtained from the TCGA skin cutanesous melanoma (TCGA-SKCM) dataset to assess the frequency and determine the clinical implications of genomic events found in the presented study. ARHGAP35 (p.L1022M) was the only mutation shared across temporal PMME lesions. The PMME samples showed higher levels of genetic instability and intra-tumor heterogeneity. They also shared several concordant copy number variations (CNV). All lesions were concordant with the evolution trajectory, and shrinkage of the founding clone caused the subclonal population to become dominant in PT1c, which was likely the reason behind metastatic seeding. ARHGAP35 mutations were found in 6% of the TCGA-SKCM cohort samples. The presence of the mutations was associated with poor progression-free survival (PFS) by both univariate and multivariate Cox regression analyses. Our study showed that the primary tumor clone disseminates earlier in PMME. This highlights the need to understand the mechanism involved in the early PMME recurrence to optimize treatment.

Hemophagocytic lymphohistiocytosis is an extremely rare occurrence during pregnancy. Early recognition of its signs and symptoms is critical for early intervention, and delays in diagnosis may be life-threatening. A 23-year-old nulliparous woman presented with a persistent fever as high as 39°C with bilateral edema of the lower limbs at 24 weeks of gestation. Typical laboratory findings included pancytopenia, high triglycerides, ferritin, transaminases, bilirubin, and hypoproteinemia. Active systemic lupus erythematosus was diagnosed using an autoimmune work-up and a Systemic Lupus Erythematosus Disease Activity Index 2000 score of 17 points. Her bone marrow aspirate revealed prominent hemophagocytosis; hence, HLH was confirmed. Genetic tests showed mutations in Syntaxin 11 mutations. Considering the potential impact of drugs on the fetus, the patient and her family members chose to terminate the pregnancy through medical induction of labor. Afterwards, her condition improved with immunosuppressive therapy.

To study the different mechanisms of understanding figurative language in a speaker’s native language (L1) and their second language (L2), this study investigated how scientific metaphors in Chinese (L1) and English (L2) are electrophysiologically processed via event-related potential experimentation. Compared with the metaphors from daily life or in literary works, scientific metaphors tend to involve both a more complicated context structure and a distinct knowledge-inferencing process. During the N400 time window (300–500 ms), English scientific metaphors elicited more negative N400s than Chinese ones at the parietal region. In the late positive component (LPC) time window (550–800 ms), English scientific metaphors elicited less positive LPCs than Chinese ones at the parietal region, and larger late negativities encompassing smaller areas of the brain. The findings might indicate that for late unbalanced bilingual speakers, L2 scientific metaphor comprehension requires more effort in information retrieval or access to the non-literal route. Altogether, the possible findings are that non-native and non-dominant language processing involves decreased automaticity of cognitive mechanisms, and decreased sensitivity to the levels of conventionality of metaphoric meanings.

PurposeTo investigate the characteristics of cerebral perfusion and hemodynamics of bypass grafting in the treatment of moyamoya disease (MMD) using the iFlow color-coded flow map in comparison with magnetic resonance imaging–perfusion-weighted imaging (MRI–PWI) and computational fluid dynamic (CFD) analysis.Materials and MethodsPatients with MMD treated with bypass grafting who had undergone MRI PWI and digital subtraction angiography for iFlow color-coded map was retrospectively enrolled and CFD was performed for calculating the hemodynamic stresses around the bypass grafting.ResultsForty-five patients with unilateral MMD treated with bypass surgery were enrolled. The bypass surgery was successful in all patients, with no severe neurological complications during the periprocedural period. Followed up for 4–12 months (median 5.5), the neurological function was good in all patients. The cerebral blood flow (CBF), cerebral blood volume (CBV), mean transit time (MTT), and time to peak (TTP) were significantly (p < 0.05) improved in the middle cerebral artery distribution area on the surgical side before and after vascular bypass, and the difference of TTP (s) measured from the proximal bifurcation of common carotid artery to the confluence of sinus was also significant (p < 0.05). A significant (p < 0.05) positive correlation existed in the perfusion parameters between the iFlow blood perfusion and the MRI–PWI perfusion, with r-value for TTP of 0.765 (p < 0.01). The iFlow color-coded blood flow map showed warm color changes on the diseased side, similar to those on the contralateral side. In CFD analysis, the hemodynamic stresses were all improved, in and around the bypass grafting and distal vessels, which were beneficial to blood flow entering distal arterial branches.ConclusionThe iFlow color-coded flow map can be used to analyze cerebral perfusion after bypass grafting for MMD, similar to MRI–PWI, and CFD can be used to analyze the hemodynamics after bypass grafting, revealing improved hemodynamics to promote blood flow entering distal arteries.

A four-week feeding trial, followed by a challenge test, was conducted to evaluate the effects of Lactococcus lactis and astaxanthin on the growth performance, digestive ability, antioxidant capacity, immunity, and disease resistance of Litopenaeus vannamei against Vibrio alginolyticus. Specifically, shrimp were randomly allocated into 12 tanks (100 shrimp per tank), and divided into 4 groups with 3 replicates. Shrimp were fed on either a control (CK) or three other treatment diets by supplementing L. lactis at 1 × 108 CFU g−1 diet (L), astaxanthin at 250 mg kg−1 diet (A) and L. lactis at 1 × 108 CFU g−1 diet combined with astaxanthin 250 mg kg−1 diet (LA). Results indicated that treatment diets significantly improved growth performance and increased the activity of digestive enzymes relative to the control diet. Particularly, the highest parameters were recorded in shrimp fed on astaxanthin alone. Moreover, statistical analysis revealed significant differences in antioxidant and immune enzymes activities between shrimp in the CK and A groups. From the results of the challenge test, shrimp fed on treatment diets all had significantly lower mortality as compared to the control diet, and shrimp fed on astaxanthin alone had the lowest mortality. Dietary supplementation of astaxanthin alone significantly increased the relative expression of Cru, Pen-3α, SOD and LZM and decreased the relative expression of proPO in the gut of shrimp as compared to the control diet. The results indicates that the relative expression levels of most selected immune-related genes were significantly up-regulated in shrimp fed on astaxanthin alone, which may explain the best disease resistance observed in this group. Taken together, the findings of this study suggest that astaxanthin can positively affect the growth performance, digestive ability, antioxidant capacity, immunity, and disease resistance of L. vannamei against V. alginolyticus. Notably, these effects are superior to those induced by L. lactis alone or in combination with L. lactis.

Background With the use of immune-checkpoint inhibitors (ICIs) in advanced or metastatic non-small cell lung cancer (NSCLC), whether ICIs or chemotherapy is more effective still remains controversial. This study was conducted to evaluate the efficacy of programmed cell death 1 (PD-1), programmed cell death ligand 1 (PD-L1), cytotoxic T-lymphocyte protein 4 (CTLA-4) alone or in their combination vs chemotherapy in patients with advanced or metastatic NSCLC. Methods This meta-analysis was conducted from PubMed, Web of Science, Medline, Embase, and the Cochrane Library up to March 2021 to identify relevant randomized controlled trials. Primary endpoints were overall survival (OS) and progression-free survival (PFS). Secondary endpoint was adverse events (AEs). This meta-analysis’s Prospero registration number is CRD42022323570. Results The search process has identified 13 studies containing 7918 patients with advanced or metastatic NSCLC. The benefit of PD-1/L1 or CTLA-4 inhibitors alone or in combination compared with chemotherapy for advanced or metastatic NSCLC was elucidated in both OS [HR = .75, 95% CI (.70-.80), P < .001] and PFS [HR = .83, 95% CI (.73-.95), P < .001]. Besides, ICIs were associated with fewer AEs compared to chemotherapy. Conclusion PD-1/L1 or CTLA-4 inhibitors alone or in combination, with fewer AEs, was associated with significant improvements in terms of OS and PFS than chemotherapy in advanced or metastatic NSCLC.

Co- and Ni-free disordered rocksalt cathodes utilize oxygen redox to increase the energy density of lithium-ion batteries, but it is challenging to achieve good cycle life at high voltages >4.5 V (versus Li/Li+). Here we report a family of Li-excess Mn-rich cathodes that integrates rocksalt- and polyanion-type structures. Following design rules for cation filling and ordering, we demonstrate the bulk incorporation of polyanion groups into the rocksalt lattice. This integration bridges the two primary families of lithium-ion battery cathodes—layered/spinel and phosphate oxides—dramatically enhancing the cycling stability of disordered rocksalt cathodes with 4.8 V upper cut-off voltage. The cathode exhibits high gravimetric energy densities above 1,100 Wh kg−1 and >70% retention over 100 cycles. This study opens up a broad compositional space for developing battery cathodes using earth-abundant elements such as Mn and Fe.

Abstract Background In recent years, excellent results have suggested an association between the “brain-gut” axis and Alzheimer’s disease (AD) progression, yet the role of the “brain-gut” axis in AD pathogenesis still remains obscure. Herein, we provided a potential link between the central and peripheral neuroinflammatory disorders in AD progression. Methods The Morris water maze (MWM) test, immunohistochemistry, ELISA, ProcartaPlex Multiplex immunoassay, multiple LC-MS/MS methods, and the V3-V4 regions of 16S rRNA genes were applied to explore potential biomarkers. Results In Tg-APP/PS1 mice, gut dysbiosis and lipid metabolism were highly associated with AD-like neuroinflammation. The combination of inflammatory factors (IL-6 and INF-γ), phosphatidylcholines (PCs) and SCFA-producing bacteria were expected to be early diagnostic biomarkers for AD. Huanglian Jiedu decoction (HLJDD) suppressed gut dysbiosis and the associated Aβ accumulation, harnessed neuroinflammation and reversed cognitive impairment. Conclusion Together, our findings highlighted the roles of neuroinflammation induced by gut dysbiosis and lipid metabolism disorder in AD progression. This integrated metabolomics approach showed its potential to understand the complex mechanisms of HLJDD in the treatment of AD.

Abstract A five‐level inverter composed of a single capacitor clamped three‐level topology and a half‐bridge is introduced, and a new SPWM control strategy for this topology is proposed. The strategy uses two modulation waves with opposite directions to modulate the positive and negative half‐cycle interleaved carriers. By controlling the charging and discharging times of the clamping capacitor in each carrier cycle, the voltage balance control of the clamping capacitor is achieved. At the same time, a single‐phase capacitor clamped five‐level inverter is taken as an example to analyse the principle of the capacitor balance control strategy in detail, and to verify the correctness and feasibility of the control strategy through simulation research and experimental results.