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Additional file 1: Figure S1. Efficiency determination of lentiviral transfection. a The structure diagram of packaged lentiviruses. b Microscopic images of transfected hASCs with GFP-tagged lentiviruses under the ordinary (left panel) and fluorescent light (right panel). Scale bar = 100 μm. c Relative expression analysis of miR-137 by qRT-PCR in transfected hASCs on 3 d, 7 d and 14 d. Data are shown as mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 versus respective NC group.

Additional file 5: Table S1. Sequences for lentiviral vectors.

Additional file 7: Table S3. Key resources table.

Additional file 2: Figure S2. MiR-137 reversely regulates hASC differentiation along osteoblastic lineage in vitro. a, b ALP staining (a) and quantification (b) of transfected hASCs after a 7-day culture in PM or OM (scale bar = 100 μm). c, d ARS staining (c) and quantification (d) of transfected hASCs after a 14-day culture in PM or OM. e Relative expression analyses of RUNX2, ALP and OCN by qRT-PCR in transfected hASCs on 3 d, 7 d and 14 d. Data are shown as mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 versus respective NC group.

Additional file 6: Table S2. Sequences of the primers for qRT-PCR.

Additional file 4: Figure S4. LSD1 knockdown activates BMP2-SMAD4 pathway and osteogenesis-associated genes expression. a Relative expression analyses of BMP2, SMAD4, RUNX2 and ALP by qRT-PCR in transfected hASCs on 3 d, 7 d and 14 d. b, c Western blotting (b) and band intensity analyses (c) of LSD1, BMP2, SMAD4 and RUNX2 in transfected hASCs on 7 d. Data are shown as mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 versus respective NC group.

Additional file 3: Figure S3. MiR-137 reversely regulates hASC differentiation along osteoblastic lineage in vivo. a Schema of the experimental design for in vivo study. n = 6 per group. b Representative soft X-ray photographs of the specimens which were subcutaneously harvested from the dorsal pockets of nude mice 8 weeks later. c Mean density analyses by the application of ImageJ software. Data are shown as mean ± SD of six independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus NC group. d Heterotopic bone formation was evaluated by histological stainings: HE, Masson trichrome staining (scale bar = 50 μm), and IHC staining of OCN (scale bar = 20 μm). Typical dark brown particles indicating OCN depositions in hASCs were marked with black arrows.

The damage detection of the wind turbine (WT) blade is an important aspect of its health situation monitoring. In order to catch small damages, it is necessary to stitch the multi segment images of the blade to obtain a high resolution image. In image stitching, a blurring of the image is carried out to reduce the influence of noise. However, the preprocessing may decrease the amount of feature points and cannot avoid the interaction of feature points in the background and the target parts. To overcome these problems, a preprocessing method based on background removal is proposed. The idea of the method is to carry out image segmentation to get the background part, then the intensities of pixels in background part are replaced by a same value, such as average intensity. Employing the proposed preprocessing method, the image stitching is implemented on several groups of WT blade images. Feature points extraction result are compared between the proposed and the blurring preprocessing, which shows that the proposed method is more robust to the parameters in image stitching process. Moreover, the capability about image stitching and multi-image stitching of the proposed method is well verified by the experiment results.

A new regeneration method was developed for reactivating poisoned commercial catalysts (V2O5-WO3/TiO2) for the NH3-SCR reaction. The proposed regeneration method can wash out poisons in relatively mild conditions, and also in situ enhance their further resistance towards alkali poisons, SO2 and H2O. To the best of our knowledge, no examples of regeneration methods with these dual functions have been reported. Characterization results showed that the new regeneration method reduces the loss of active components and significantly improves the surface acidity, thus enhancing the resistance to poisons in further deNOx operation.