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1,075 results on '"Waldhäusl, W."'

155. Polar secretion of endothelin-1 by cultured endothelial cells

Author

Of, Wagner, Christ G, Johann Wojta, Vierhapper H, Parzer S, Pj, Nowotny, Schneider B, Waldhäusl W, and Br, Binder

Subjects
Kinetics, Umbilical Veins, Cell Membrane Permeability, Pregnancy, Endothelins, Cell Membrane, Humans, Female, Amnion, Endothelium, Vascular, Cells, Cultured, Dexamethasone
Abstract

The aim of this study was to determine the permeability of endothelial monolayers for endothelin-1 and a possible directionality of the endothelin-1 secretion process. Human umbilical vein endothelial cells were cultured on acellular amniotic membranes, dividing the tissue culture wells into an apical (luminal) and a basolateral (abluminal) compartment. Whereas in the absence of endothelial monolayers 44.9 +/- 2.3 and 43.5 +/- 2.0% of the unilaterally added endothelin-1 permeated from the apical to the basolateral side and from the basolateral to the apical side, respectively, only 6.5 +/- 0.6 and 6.6 +/- 0.4% diffused in the presence of endothelial cells. Analyzing endothelin-1 secretion, approximately 80% of the total amount of synthesized endothelin-1 was found in the basolateral compartment; thrombin (10 units/ml) stimulated the production of endothelin-1 approximately 2-fold, but did not change the relative distribution of endothelin-1 between the apical and basolateral compartments. In the presence of dexamethasone (10(-7) M), a decrease in the level of endothelin-1 was found in the apical compartment, whereas the total amount of endothelin-1 produced was not affected. Dexamethasone did not influence the permeability of human umbilical vein endothelial cell monolayers for endothelin-1. These results strongly support the hypothesis that endothelin-1 is a local paracrine regulator of vasotone.

Published
1992

164. Suramin and the human adrenocortex: results of experimental and clinical studies

Author

Dorfinger K, Bruno Niederle, Vierhapper H, Astrid W, Czernin S, Nowotny P, Waldhäusl W, and Grubeck-Loebenstein B

Subjects
Adrenocorticotropic Hormone, Hydrocortisone, Pregnenolone, Adrenal Cortex, Humans, Pilot Projects, Suramin, Cell Division, Cells, Cultured
Abstract

Suramin, a sulfonated drug, has been used successfully in the treatment of inoperable adrenocortical cancer. This study was undertaken to investigate the effects of suramin on the basal and the adrenocorticotropin-stimulated cortisol and pregnenolone secretion and on the proliferation of primary monolayer cultures of normal human adrenocortical cells. Suramin decreases basal and adrenocorticotropin-stimulated cortisol secretion in a dose-dependent manner (p less than 0.05 from 0.3 mmol/L upward). At a suramin concentration of 3 mmol/L cortisol, secretion was inhibited by 70% +/- 4% in adrenocorticotropin-stimulated cells and by 42% +/- 6% in unstimulated cells. The proliferation of adrenocortical cells in response to fetal calf serum was inhibited by suramin at concentrations from 0.3 mmol/L upward, maximal suppression (71% +/- 6%; p less than 0.05) being observed at a concentration of 10 mmol/L. Neither down-regulation of cortisol secretion nor inhibition of adrenocortical cell proliferation was caused by toxicity of the compound, as could be shown by adrenocorticotropin-restimulating cortisol secretion in suramin-treated cells. The results indicate that suramin exerts an inhibitory influence on the cortisol secretion and the proliferation of normal human adrenocortical cells and may be useful in treating adrenocortical cancer.

Published
1991

165. The world of Diabetologia in 1999

Author

Waldhäusl W

Subjects
Gerontology, business.industry, Endocrinology, Diabetes and Metabolism, MEDLINE, medicine.disease, Europe, Text mining, Diabetes mellitus, Diabetes Mellitus, Internal Medicine, Humans, Medicine, Periodicals as Topic, business
Published
1999
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