151. Sirtuin 1 Regulates SREBP-1c Expression in a LXR-Dependent Manner in Skeletal Muscle
- Author
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Andréa Castro Perez, Yann S. Gallot, Kevin Dessalle, Josiane Castells, Vanessa Euthine, Yansong Gu, Etienne Lefai, Christine Durand, Aurelia Defour, Thomas Poyot, Daniel Béchet, Damien Freyssenet, André Peinnequin, Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Physiologie de l'Exercice (LPE), Université Jean Monnet - Saint-Étienne (UJM), Centre de Recherches du Service de Santé des Armées (CRSSA), Service de Santé des Armées, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Caisse d'Epargne Rhone-Alpes, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Laboratoire de Physiologie de l'Exercice EA4338 (LPE), Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National de la Recherche Agronomique (INRA)
- Subjects
Male ,Anatomy and Physiology ,Mouse ,lcsh:Medicine ,Gene Expression ,Biochemistry ,Transactivation ,Mice ,0302 clinical medicine ,Sirtuin 1 ,Molecular Cell Biology ,polycyclic compounds ,Myocyte ,lcsh:Science ,Promoter Regions, Genetic ,Musculoskeletal System ,Liver X Receptors ,Regulation of gene expression ,0303 health sciences ,Multidisciplinary ,biology ,Myogenesis ,food and beverages ,muscle squelettique ,Acetylation ,Animal Models ,acétylation ,Orphan Nuclear Receptors ,Cell biology ,medicine.anatomical_structure ,Mammalogy ,030220 oncology & carcinogenesis ,Muscle ,lipids (amino acids, peptides, and proteins) ,ciblage génique ,Sterol Regulatory Element Binding Protein 1 ,hormones, hormone substitutes, and hormone antagonists ,expression des gènes ,Research Article ,Transcriptional Activation ,03 medical and health sciences ,Model Organisms ,transactivation ,DNA-binding proteins ,medicine ,Genetics ,Animals ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Liver X receptor ,Muscle, Skeletal ,Biology ,muscle gastrocnémien ,lipide ,030304 developmental biology ,Muscle Cells ,lcsh:R ,Skeletal muscle ,Proteins ,Molecular biology ,Sterol regulatory element-binding protein ,Metabolism ,Gene Expression Regulation ,biology.protein ,lcsh:Q ,Gene Function ,facteur de transcription ,Zoology - Abstract
International audience; Sirtuin 1 (SIRT1), a NAD +-dependent protein deacetylase, has emerged as a main determinant of whole body homeostasis in mammals by regulating a large spectrum of transcriptional regulators in metabolically relevant tissue such as liver, adipose tissue and skeletal muscle. Sterol regulatory element binding protein (SREBP)-1c is a transcription factor that controls the expression of genes related to fatty acid and triglyceride synthesis in tissues with high lipid synthesis rates such as adipose tissue and liver. Previous studies indicate that SIRT1 can regulate the expression and function of SREBP-1c in liver. In the present study, we determined whether SIRT1 regulates SREBP-1c expression in skeletal muscle. SREBP-1c mRNA and protein levels were decreased in the gastrocnemius muscle of mice harboring deletion of the catalytic domain of SIRT1 (SIRT1 Dex4/Dex4 mice). By contrast, adenoviral expression of SIRT1 in human myotubes increased SREBP-1c mRNA and protein levels. Importantly, SREBP-1c promoter transactivation, which was significantly increased in response to SIRT1 overexpression by gene electrotransfer in skeletal muscle, was completely abolished when liver X receptor (LXR) response elements were deleted. Finally, our in vivo data from SIRT1 Dex4/Dex4 mice and in vitro data from human myotubes overexpressing SIRT1 show that SIRT1 regulates LXR acetylation in skeletal muscle cells. This suggests a possible mechanism by which the regulation of SREBP-1c gene expression by SIRT1 may require the deacetylation of LXR transcription factors.
- Published
- 2012