151. Significance of uncoupling protein 3 in mitochondrial function upon mid- and long-term dietary high-fat exposure
- Author
-
Nabben, Miranda, Hoeks, Joris, Moonen-Kornips, Esther, van Beurden, Denis, Briedé, Jacob J., Hesselink, Matthijs K.C., Glatz, Jan F.C., and Schrauwen, Patrick
- Subjects
- *
LABORATORY mice , *LIPID metabolism , *MEMBRANE proteins , *SKELETAL muscle , *MITOCHONDRIAL DNA abnormalities , *HIGH-fat diet - Abstract
Abstract: Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production after short-term (8weeks) high-fat feeding. After 26weeks of high-fat feeding, UCP3 knockout mice exhibited reduced mitochondrial function as measured ex vivo in isolated mitochondria. In conclusion, these data suggest that UCP3 may have a role in the protection of mitochondria against lipid-induced mitochondrial dysfunction, but only after long-term exposure to high-fat. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF