470 results on '"Tufano, M"'
Search Results
152. Suppression of rat epididymal sperm immunogenicity by a seminal vesicle secretory protein and transglutaminase both in vivo and in vitro
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Peluso, G., Raffaele Porta, Esposito, C., Tufano, M. A., Toraldo, R., Vuotto, M. L., Ravagnan, G., Metafora, S., Peluso, G, Porta, R, Esposito, C, Tufano, Ma, Toraldo, Roberto, Vuotto, Ml, Ravagnan, G, and Metafora, S.
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Epididymis ,Male ,Antigen Presentation ,Transglutaminases ,Histocompatibility Antigens Class II ,Seminal Plasma Proteins ,Prostatic Secretory Proteins ,Proteins ,Seminal Vesicles ,Macrophage Activation ,Spermatozoa ,Epithelium ,Rats ,Phagocytosis ,Superoxides ,Rats, Inbred BN ,Macrophages, Peritoneal ,Animals ,Female ,Rats, Wistar - Abstract
The pretreatment of epididymal spermatozoa with SV-IV, one of the major secretory protein produced by the epithelium of adult rat seminal vesicles, was found to markedly decrease their ability to induce in vivo peritoneal macrophage activation, measured as class II major histocompatibility complex surface antigen expression, superoxide anion production, phagocytic activity, and antigen presentation. In addition, the treatment of spermatozoa with SV-IV produced a significant decrease of their immunogenicity evaluated in vitro by [3H]thymidine incorporation in splenocyte/spermatozoon co-culture. The concurrent presence of SV-IV and transglutaminase, an enzyme secreted in large amounts from the rat anterior prostate, amplified these phenomena. The suppression of the epididymal sperm immunogenicity is suggested to be of crucial importance for the prevention of the immune response to the sperm introduced in the immunocompetent female genital tract during coitus.
153. Quinine sulfate and HSV replication
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Wolf, R., Baroni, A., Greco, R., federica corrado, Ruocco, E., Tufano, M. A., Ruocco, V., Wolf, R, Baroni, Adone, Greco, R, Corrado, F, Ruocco, Eleonora, Tufano, Ma, and Ruocco, Vincenzo
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Antimalarials ,Quinine ,Chlorocebus aethiops ,Animals ,Herpesvirus 1, Human ,Virus Replication ,Vero Cells - Abstract
Although antimalarial drugs have been developed primarily to treat malaria, they are also beneficial for many dermatological, immunological, and rheumatological diseases, for which they are mostly used today in the Western world. The aim of the present study was to investigate the effect of quinine sulfate (QS) on the multiplication and adsorption of herpes virus type I (HSV-1). When Vero cells (African green monkey kidney) are infected with HSV-1 in the presence of QS, the viral adsorption is reduced, as demonstrated by a decrease of the number of microscopic plaques of the virus. When the virus-infected Vero cells are incubated in the presence of QS, the multiplication of HSV-1 is also reduced, and the diameter of the plaque are visibly smaller. The practical implications of the antiviral action of antimalarial drugs might be especially important to immunosuppressed patients who receive these drugs for autoimmune collagen-vascular diseases or as additional therapy for AIDS.
154. Neonatal cholestasis: A great pantomime,Colestasi neonatale: La grande simulatrice
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Tufano, M., Francesco Cirillo, Ranucci, G., Vecchione, R., Sonzogni, A., Alberti, D., and Iorio, R.
155. Malassezia, dermatitis and innate immunity | Malassezia, dermatoses et immunité innée
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Elisabetta Buommino, Donnarumma, G., Tufano, M. A., and Baroni, A.
156. Salmonella typhimurium porins stimulate platelet-activating factor synthesis by human polymorphonuclear neutrophils
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Tufano, M. A., Tetta, C., Biancone, L., Eugenio Luigi Iorio, Baroni, A., Giovane, A., Camussi, G., Tufano, Ma, Tetta, C, Biancone, L, Iorio, El, Baroni, Adone, Giovane, Alfonso, and Camussi, G.
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Salmonella typhimurium ,platelet-activating factor ,Arachidonic Acid ,Time Factors ,Dose-Response Relationship, Drug ,porins ,Neutrophils ,Immunology ,In Vitro Techniques ,Enzyme Activation ,Immunology and Allergy ,Humans ,Calcium ,Platelet Activating Factor ,Extracellular Space ,Bacterial Outer Membrane Proteins - Abstract
Porins, a family of hydrophobic proteins located in the outer membrane of cell-wall of Gram-negative bacteria, were shown to stimulate the synthesis and release of platelet-activating factor (PAF), a 1-O-alkyl-2-acetyl-sn-glycerol-3-phosphorylcholine mediator of inflammation and endotoxic shock produced by polymorphonuclear neutrophils. PAF synthesis was independent either from contamination by LPS or generation of TNF. Experiments with labeled precursors demonstrated that PAF was synthesized via the remodeling pathway that involves acetylation of 1-O-alkyl-sn-glyceryl-3-phosphorylcholine generated from 1-O-alkyl-2-acyl-sn-glyceryl-3-phosphorylcholine by phospholipase A2 (PLA2) activity. Porins, indeed, induced a sustained PLA2-dependent mobilization of [14C]arachidonic acid that was inhibited by p-bromodiphenacylbromide. p-Bromodiphenacylbromide, an inhibitor of PLA2, also blocked PAF synthesis by preventing the mobilization of 2-lyso-PAF, the substrate for PAF-specific acetyltransferase. The addition of 2-lyso-PAF restored PAF synthesis. The activity of acetyl CoA:2-lyso-PAF acetyltransferase was transiently increased in porin-stimulated PMN and the [3H]acetyl group was incorporated in the synthetized PAF after cell preincubation with [3H]acetyl CoA. The activation of PAF synthesis by porins as well as its release were dependent on extracellular Ca2+. Porins by forming trans-membrane channels determined a sustained influx of 45Ca2+ into the cytosol. As shown by inhibitors of Ca(2+)-calmodulin complexes, calmodulin mediated the Ca(2+)-dependent activation of enzymes involved in PAF synthesis.
157. A boy with urso-dependent hypertransaminasemia,Un'ipertransaminasemia urso-dipendente
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Ranucci, G., Cirillo, F., Lorenzo D'Antiga, Tufano, M., D Ambrosi, M., Giordano, G., and Iorio, R.
158. ChemInform Abstract: 8-METHYLENE-EXO-3,3-DIPHENYLTRICYCLO(3.2.1.02,4)OCTANE, A PROBE FOR ADDITION REACTION MECHANISM
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WILT, J. W., primary and TUFANO, M. D., additional
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- 1985
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159. Affinity of various cations forStaphylococcus aureuscell-wall
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Galdiero, F., Lembo, M., and Tufano, M. A.
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- 1968
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160. ChemInform Abstract: Novel Methodology for the Synthesis of trans-Alkene Dipeptide Isosteres.
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SHUE, Y.-K., CARRERA, G. M. JUN., TUFANO, M. D., and NADZAN, A. M.
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- 1991
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161. Environmental management of waters and riparian areas to protect biodiversity through River Contracts: The experience of Tiber River (Rome, Italy)
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Maria Cristina Tullio, Giulia Caneva, Simona Ceschin, Corrado Battisti, M Tufano, Fernando Lucchese, Emanuela Cicinelli, Massimiliano Scalici, Caneva, G., Ceschin, S., Lucchese, F., Scalici, M., Battisti, C., Tufano, M., Tullio, M. C., and Cicinelli, E.
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geography.geographical_feature_category ,business.industry ,Environmental resource management ,Biodiversity ,nature conservation ,urban planning ,ecosystem service ,Ecosystem services ,Geography ,Urban planning ,Nature Conservation ,Environmental Chemistry ,River management ,business ,Tiber River ,river management ,General Environmental Science ,Water Science and Technology ,Riparian zone - Abstract
Urban rivers may perform important ecological roles and provide many ecosystem services, especially in urban contexts. Considering this, River Contracts were officially introduced in 2000, during the Second Forum of the World Water Council, as participative agreements aimed at managing the complexity of fluvial ecosystems. Collecting data on fluvial and riparian biodiversity, establishing ecological relationships among biological components, and evaluating their resistance and resilience to anthropic impacts are needed to assess the ecological sustainability of the anthropic actions. Here, we provide a review of literature and regulation on River Contracts, and a methodological contribution to the evaluation of different environmental components, their ecological connections, and the role of anthropic impacts. To address the aim, we performed a critical analysis of the existing data on the Tiber River close to Rome. We used the information to identify the actions that should be included in the Tiber River Contract to improve its natural value and its potential ecosystem services. Several areas emerged where information was lacking such as the human impact on these ecosystems. Data highlight the disappearance of rare Mediterranean species and of those linked to wetland habitats, as well as an increase in alien species and species typical of disturbed areas. Further research on biodiversity and management is required to support nature conservation.
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- 2021
162. Cell Injury and cell death
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Eleonora Ruocco, Maria Antonietta Tufano, Elisabetta Buommino, Adone Baroni, RONNI WOLF ET ALL, Baroni, Adone, Ruocco, Eleonora, Tufano, M. A., Buommino, Elisabetta, R.Wolf, LC.Parrish, J.L.Parrish, Baroni, A., Ruocco, E., Buommino, E., BARONI A, RUOCCO E, TUFANO M.A, BUOMMINO E, Ronni Wolf, Baroni, A, Ruocco, E, and Tufano, M
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Programmed cell death ,Necrosis ,biology ,Cell growth ,Cellular differentiation ,Cell ,Calpain ,Cell biology ,Paraptosis ,medicine.anatomical_structure ,Apoptosis ,medicine ,biology.protein ,medicine.symptom - Abstract
WHEN CELLS are damaged, as often occurs during trauma and metabolic stress, the organism has to choose whether to repair the damage by promoting cell survival or remove irreparably injured cells. Cell injury occurs when an adverse stimulus reversibly disrupts the normal, complex homeostatic balance of the cellular metabolism. In this case, after injury the cells attempt to seal breaks in their membranes, chaperone the removal or refolding of altered proteins, and repair damaged DNA. On the contrary, when cell injury is too extensive to permit reparative responses, the cell reaches a “point of no return” and the irreversible injury culminates in programmed cell death (PCD). Specific properties or features of cells make them more or less vulnerable to external stimuli, thus determining the kind of cellular response. In addition, the characteristic of the injury (type of injury, exposure time, or severity) will also affect the extent of the damage. We present a short overview of the best-known PCD pathways. We emphasize the apoptotic pathway, considered for years the hallmark of PCD, and the different stimuli that produce cell injury. CELL INJURY The survival of multicellular organisms depends on the function of a diverse set of differentiated cell types. After development is complete, the viability of the organism depends on the maintenance and renewal of these diverse lineages. Within each lineage homeostasis is maintained through a delicate balance between cell proliferation and cell death.
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- 2010
163. 3-O-METHYLFUNICONE, A METABOLITE FROM PENICILLIUM PINOPHILUM, INHIBITS PROLIFERATION OF HUMAN MELANOMA CELLS BY CAUSING G2/M ARREST AND INDUCING APOPTOSIS
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Adone Baroni, Lucrezia Manente, Marcella Petrazzuolo, A. De Filippis, A. De Luca, Rosario Nicoletti, Elisabetta Buommino, Maria Antonietta Tufano, Baroni, Adone, DE LUCA, Antonio, DE FILIPPIS, A., Petrazzuolo, M., Manente, L., Nicoletti, R., Tufano, M. A., Buommino, Elisabetta, Baroni, A, De Luca, A, De Filippis, A, Petrazzuolo, M, Manente, L, Nicoletti, R, and Tufano, M. A
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rho GTP-Binding Proteins ,Programmed cell death ,Cell ,Poly (ADP-Ribose) Polymerase-1 ,Apoptosis ,Biology ,Flow cytometry ,Cell Line, Tumor ,Survivin ,medicine ,Humans ,RNA, Small Interfering ,Cyclin B1 ,Poly(ADP-ribose) Polymerase ,Melanoma ,Pyrone ,Cell Proliferation ,medicine.diagnostic_test ,Caspase 3 ,Cell growth ,Cell Cycle ,Penicillium ,Apoptosi ,Original Articles ,Cell Biology ,General Medicine ,Caspase 9 ,Cell biology ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,Pyrones ,Cell culture ,Cancer research ,Poly(ADP-ribose) Polymerases ,Human - Abstract
Objectives: Melanoma cells take advantage of impaired ability to undergo programmed cell death in response to different external stimuli and chemotherapeutic drugs; this makes prevention of tumour progression very difficult. The aim of this study was to demonstrate whether 3-O-methylfunicone (OMF), a metabolite of Penicillium pinophilum, has the ability to arrest cell population growth and to induce apoptosis in A375P (parental) and A375M (metastasis derivatived) melanoma cell lines. Materials and methods: Cell proliferation and apoptosis were analysed by flow cytometry, DNA fragmentation, caspase-3 and caspase-9 activation, and PARP-1 cleavage. Results: We demonstrated that OMF affected cell proliferation in a time- and dose-dependent manner, reaching the best effect at concentration of 80 g/ml for 24 h. Flow cytometry revealed that OMF caused significant G2 phase arrest, which was associated with marked decrease in cyclin B1/p34cdc2 complex and p21 induction. OMF also induced marked decrease of survivin expression. Reduced levels of apoptosis were evident after silencing p21 expression in both cell lines. Finally, the effect exercised by OMF on hTERT and TEP-1 gene expression confirmed the ability of this molecule to interfere with replicative ability of cells. Conclusions: The results reported here seem to suggest that OMF as a promising molecule to include in strategies for treatment of melanoma. © 2009 Blackwell Publishing Ltd.
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- 2009
164. Alternative macrophage polarisation associated with resistance to anti-PD1 blockade is possibly supported by the splicing of FKBP51 immunophilin in melanoma patients
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Fortunato Ciardiello, Paolo D’ Arrigo, Emilio Francesco Giunta, Giuseppe Argenziano, Vincenza Vigorito, Martina Tufano, Teresa Troiani, Simona Romano, Maria Romano, Troiani, Teresa, Giunta, Emilio Francesco, Tufano, Martina, Vigorito, Vincenza, D'Arrigo, Paolo, Argenziano, Giuseppe, Ciardiello, Fortunato, Romano, MARIA FIAMMETTA, Romano, Simona, Troiani, T., Giunta, E. F., Tufano, M., Vigorito, V., Arrigo, P. D., Argenziano, G., Ciardiello, F., Romano, M. F., and Romano, S.
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Male ,Cancer Research ,Immune receptor ,Antibodies, Monoclonal, Humanized ,Peripheral blood mononuclear cell ,T-Lymphocytes, Regulatory ,Article ,B7-H1 Antigen ,Flow cytometry ,Tacrolimus Binding Proteins ,03 medical and health sciences ,Prognostic markers ,0302 clinical medicine ,Immunophenotyping ,Immune system ,medicine ,Gene silencing ,Humans ,Protein Isoforms ,Melanoma ,Immune Checkpoint Inhibitors ,030304 developmental biology ,Aged ,0303 health sciences ,medicine.diagnostic_test ,business.industry ,Monocyte ,Macrophages ,Macrophage Activation ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,Nivolumab ,Oncology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer research ,Female ,Immunotherapy ,business - Abstract
Background FKBP51 immunophilin is abundantly expressed by immune cells. Co-inhibitory immune receptor signalling generates the splicing isoform FKBP51s. Tregs stained by FKBP51s are increased in melanoma patients and their counts are associated with anti-CTLA-4 response. An expansion of FKBP51s+PD-L1+ monocytes was measured in a group of non-responding patients to anti-CTLA-4. The aim of this work was to confirm the predictive value of response of FKBP51s+Tregs in a cohort of patients undergoing anti-PD1 treatment and shed light on a monocyte subset co-expressing PD-L1/FKBP51s. Methods Co-cultures of organoids and autologous lymphocytes were used to confirm that tumour T-cell interaction can induce FKBP51s. PBMC immunophenotype and flow cytometry served to assess and monitor FKBP51s+Treg and FKBP51s+PD-L1+ monocytes in 22 advanced melanoma patients treated with anti-PD1. Silencing and overexpression of FKBP51s in human macrophages served to address the protein role in the tolerant macrophages’ behaviour. Results FKBP51s+Tregs count was increased in responders and had a prognostic value. Non-responders showed an early increase in FKBP51s+ PD-L1+ monocytes during anti-PD1 treatment. Manipulation of FKBP51s modulated the macrophage–phenotype, with forced protein expression promoting aspects associated with tolerance. Conclusions FKBP51s may guide in the selection and monitoring of melanoma patient candidates to immune-checkpoint-targeted therapy. Manipulation of FKBP51s may overcome resistance.
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- 2020
165. Cell stemness, epithelial-to-mesenchymal transition, and immunoevasion: Intertwined aspects in cancer metastasis
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Paolo D'Arrigo, Vincenza Vigorito, Maria Fiammetta Romano, Simona Romano, Salvatore Russo, Martina Tufano, Romano, Simona, Tufano, M, D'Arrigo, P, Vigorito, V, Russo, S, and Romano, Mf
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PD-L1 ,0301 basic medicine ,Cancer Research ,Epithelial-Mesenchymal Transition ,medicine.medical_treatment ,Cell ,B7-H1 Antigen ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Cancer immunotherapy ,Neoplasms ,Tumor Microenvironment ,medicine ,Humans ,Epithelial–mesenchymal transition ,Neoplasm Metastasis ,Cell Death ,biology ,Mesenchymal phenotype ,Mesenchymal stem cell ,Cancer ,medicine.disease ,Killer Cells, Natural ,030104 developmental biology ,medicine.anatomical_structure ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer stemne ,Cancer cell ,Disease Progression ,Neoplastic Stem Cells ,biology.protein ,Cancer research ,Immunoevasion ,Tumor Escape ,Disease Susceptibility - Abstract
Recent advances in tumor immunology, fostered by dramatic outcomes with cancer immunotherapy, have opened new scenarios in cancer metastasis. The cancer stemness/mesenchymal phenotype and an excess of immune suppressive signals are emerging as Intertwined aspects of human tumors. This review examines recent studies that explored the mechanistic links between cancer cell stemness and immunoevasion, and the evidence points to these key events in cancer metastasis as two sides of the same coin. This review also covers the mechanisms involved in tumor expression of programmed cell death ligand 1 (PD-L1), a major factor exploited by human neoplasias to suppress immune control. We highlight the convergence of mesenchymal traits and PD-L1 expression and examine the functions of this immune inhibitory molecule, which confers cancer cell resistance and aggressiveness.
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- 2020
166. FKBP51 Affects TNF-Related Apoptosis Inducing Ligand Response in Melanoma
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Rosanna Martinelli, Martina Tufano, Maria Romano, Roberto Pacelli, Elena Cesaro, Simona Romano, Tufano, M., Cesaro, E., Martinelli, R., Pacelli, R., Romano, S., and Romano, M. F.
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Programmed cell death ,QH301-705.5 ,Chemistry ,YY1 ,Melanoma ,Repressor ,TRAIL ,Cell Biology ,medicine.disease ,TRAIL, melanoma, cell death, FKBP51, YY1 ,Cell and Developmental Biology ,cell death ,FKBP51 ,Immune system ,Apoptosis ,Cancer cell ,melanoma ,Cancer research ,medicine ,Biology (General) ,Transcription factor ,Original Research ,Developmental Biology - Abstract
Melanoma is one of the most immunogenic tumors and has the highest potential to elicit specific adaptive antitumor immune responses. Immune cells induce apoptosis of cancer cells either by soluble factors or by triggering cell-death pathways. Melanoma cells exploit multiple mechanisms to escape immune system tumoricidal control. FKBP51 is a relevant pro-oncogenic factor of melanoma cells supporting NF-κB-mediated resistance and cancer stemness/invasion epigenetic programs. Herein, we show that FKBP51-silencing increases TNF-related apoptosis-inducing ligand (TRAIL)-R2 (DR5) expression and sensitizes melanoma cells to TRAIL-induced apoptosis. Consistent with the general increase in histone deacetylases, as by the proteomic profile, the immune precipitation assay showed decreased acetyl-Yin Yang 1 (YY1) after FKBP51 depletion, suggesting an impaired repressor activity of this transcription factor. ChIP assay supported this hypothesis. Compared with non-silenced cells, a reduced acetyl-YY1 was found on the DR5 promoter, resulting in increased DR5 transcript levels. Using Crispr/Cas9 knockout (KO) melanoma cells, we confirmed the negative regulation of DR5 by FKBP51. We also show that KO cells displayed reduced levels of acetyl-EP300 responsible for YY1 acetylation, along with reduced acetyl-YY1. Reconstituting FKBP51 levels contrasted the effects of KO on DR5, acetyl-YY1, and acetyl-EP300 levels. In conclusion, our finding shows that FKBP51 reduces DR5 expression at the transcriptional level by promoting YY1 repressor activity. Our study supports the conclusion that targeting FKBP51 increases the expression level of DR5 and sensitivity to TRAIL-induced cell death, which can improve the tumoricidal action of immune cells.
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- 2021
167. PD-L1 Expression Fluctuates Concurrently with Cyclin D in Glioblastoma Cells
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Laura Marrone, Massimo D'Agostino, Paolo D'arrigo, Elena Cesaro, Maria Romano, Martina Tufano, Carolina Giordano, Simona Romano, Tufano, M., D'Arrigo, P., D'Agostino, M., Giordano, C., Marrone, L., Cesaro, E., Romano, M. F., and Romano, S.
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PD-L1 ,QH301-705.5 ,Cyclin D ,Cell ,Biology ,Article ,B7-H1 Antigen ,Brain Neoplasm ,Cyclin D1 ,Western blot ,Cell Line, Tumor ,Gene expression ,medicine ,Humans ,Biology (General) ,Cell Proliferation ,medicine.diagnostic_test ,Brain Neoplasms ,Cell growth ,General Medicine ,Fibroblasts ,Flow Cytometry ,Molecular biology ,medicine.anatomical_structure ,Real-time polymerase chain reaction ,FKBP51 ,Cell culture ,biology.protein ,Fibroblast ,Glioblastoma - Abstract
Despite Glioblastoma (GBM) frequently expressing programmed cell death ligand-1 (PD-L1), treatment with anti-programmed cell death-1 (PD1) has not yielded brilliant results. Intratumor variability of PD-L1 can impact determination accuracy. A previous study on mouse embryonic fibroblasts (MEFs) reported a role for cyclin-D in control of PD-L1 expression. Because tumor-cell growth within a cancer is highly heterogeneous, we looked at whether PD-L1 and its cochaperone FKBP51s were influenced by cell proliferation, using U251 and SF767 GBM-cell-lines. PD-L1 was measured by Western blot, flow cytometry, confocal-microscopy, quantitative PCR (qPCR), CCND1 by qPCR, FKBP51s by Western blot and confocal-microscopy. Chromatin-Immunoprecipitation assay (xChIp) served to assess the DNA-binding of FKBP51 isoforms. In the course of cell culture, PD-L1 appeared to increase concomitantly to cyclin-D on G1/S transition, to decrease during exponential cell growth progressively. We calculated a correlation between CCND1 and PD-L1 gene expression levels. In the temporal window of PD-L1 and CCND1 peak, FKBP51s localized in ER. When cyclin-D declined, FKBP51s went nuclear. XChIp showed that FKBP51s binds CCND1 gene in a closed-chromatin configuration. Our finding suggests that the dynamism of PD-L1 expression in GBM follows cyclin-D fluctuation and raises the hypothesis that FKBP51s might participate in the events that govern cyclin-D oscillation.
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- 2021
168. Polycystic ovary syndrome in pediatric obesity and diabetes
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Enza Mozzillo, Maria Tufano, Maria Cristina Vigone, Laura Lucaccioni, Maurizio Delvecchio, Gabriella Pozzobon, Graziamaria Ubertini, Elena Dondi, Dondi, E., Tufano, M., Vigone, M. C., Lucaccioni, L., Pozzobon, G., Ubertini, G., Mozzillo, E., and Delvecchio, M.
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Adult ,Pediatrics ,medicine.medical_specialty ,Diabetes mellitu ,Hirsutism ,Pediatric Obesity ,Adolescent ,Anovulation ,Diabetes mellitus ,medicine ,Obesity ,Polycystic ovary syndrome ,Child ,Female ,Humans ,Diabetes Mellitus, Type 2 ,Polycystic Ovary Syndrome ,hirsutism ,business.industry ,Type 2 Diabetes Mellitus ,medicine.disease ,Polycystic ovary ,Metformin ,Pediatrics, Perinatology and Child Health ,Amenorrhea ,medicine.symptom ,Metabolic syndrome ,business ,Type 2 ,medicine.drug - Abstract
Introduction Polycystic ovary syndrome is characterized by anovulation (amenorrhea, oligomenorrhea, irregular menstrual cycles) combined with symptoms of androgen excess (hirsutism, acne, alopecia). The clear definition and diagnosis in adolescents could be challenging considering that most of symptoms occur as part of the expected physiological hormonal imbalance of puberty. Therefore, different diagnostic criteria have been elaborated. Polycystic ovary syndrome could be associated to obesity, diabetes mellitus, and metabolic syndrome. In adolescents with polycystic ovary syndrome, adiposity is associated with higher androgen concentrations and greater menstrual irregularity. Polycystic ovary syndrome in youth is considered a risk factor for type 2 diabetes mellitus in adulthood. On the other hand, increased prevalence of polycystic ovary syndrome has been shown in type 1 diabetes mellitus. Evidence The treatment of polycystic ovary syndrome in adolescents is controversial considering that adequate trials are lacking. First line treatment comprises lifestyle modification (preferably multicomponent including diet, exercise and behavioural strategies) that should be recommended overall in the patients with polycystic ovary syndrome and overweight, central obesity and insulin resistance. Beyond non-pharmacological therapy, pharmacological agents include combined hormonal contraceptives, metformin and antiandrogens, used separately or in combination. The aim of therapy is to bring back ovulation, to normalize menses, to reduce hirsutism and acne, to reduce weight. Other important goal is the treatment of hyperlipidaemia and of hyperglycaemia. Conclusions This narrative review aims to review the most pertinent literature about polycystic ovary syndrome in adolescents with obesity or diabetes. We overviewed the diagnostic criteria, the pathophysiology and the possible treatment approaches.
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- 2021
169. Il Patto sulle migrazioni e l’asilo della Commissione europea del 23 settembre 2020 e il dovere di soccorso in mare
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Starita M, Tufano, M, Pugliese S, D'arienzo, M, and Starita M
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Settore IUS/13 - Diritto Internazionale ,search and rescue operation ,soft law in the European Union Law, cooperazione tra gli Stati membri dell'Unione ,Pact on Asylum and migration - Abstract
The pact on asylum and migration, presented by the European Commission on 23 September 2020, addresses some of the problems related to search and rescue operations and the following disembarkations. The present paper is skeptical about the real capacity of the pact to reduce the existing tensions among Member States in this field; it also underlines the ambiguities of the pact concerning Member States’ cooperation with NGOs engaged in SAR activities
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- 2021
170. Thrombocytopenia Complicating Transcatheter Aortic Valve Implantation: Differences Between Two New-Generation Devices
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Martino Pepe, Michele Cimmino, Maria Romano, Arturo Giordano, Giuseppe Biondi-Zoccai, Martina Tufano, Nicola Corcione, Simona Romano, Daniela Capasso, Michele Albanese, Salvatore Giordano, Salvatore Buonpane, Alberto Morello, Paolo Ferraro, Corcione, N., Romano, S., Morello, A., Ferraro, P., Cimmino, M., Albanese, M., Tufano, M., Capasso, D., Buonpane, S., Giordano, S., Pepe, M., Biondi-Zoccai, G., Romano, M. F., and Giordano, A.
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Male ,medicine.medical_specialty ,Transcatheter aortic ,Inflammatory cytokine ,Pharmaceutical Science ,030204 cardiovascular system & hematology ,Prosthesis Design ,Phagocytic monocytes ,Proinflammatory cytokine ,Transcatheter Aortic Valve Replacement ,TAVI ,03 medical and health sciences ,0302 clinical medicine ,Postoperative Complications ,Internal medicine ,Genetics ,Medicine ,Humans ,Platelet ,030212 general & internal medicine ,Prospective cohort study ,Genetics (clinical) ,Retrospective Studies ,Aged, 80 and over ,Bioprosthesis ,Phagocytic monocyte ,business.industry ,Inflammatory cytokines ,Thrombocytopenia ,Serum cytokine ,Platelet cell death ,Italy ,Heart Valve Prosthesis ,Cardiology ,Molecular Medicine ,Cytokines ,Original Article ,Female ,Delivery system ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers - Abstract
Thrombocytopenia after TAVI is common and clinically detrimental. Retrospectively, we observed Portico recipients had a more profound platelet drop than Evolut recipients. We thus investigated periprocedural platelet damage and/orpro-inflammatory state in 64 TAVI recipients at baseline and after implantation. Platelet damage was assessed by annexin V staining and monocyte-phagocytic phenotype was assessed according to CD14/CD36 expression. Serum cytokines were measured in 20 patients. The formaldehyde-based storage solution altered platelets. When, before being loaded onto the delivery system, Portico underwent one additional flushing to those recommended, the receiving patients showed thrombocytopenia, platelet damage, and CD36-monocyte count were mitigated. A general increase in IL-6 was recorded in overall TAVI recipients, but a high serum level of IL-8, a potent thrombocytopenia inducer, was measured in Portico recipients only, including those with extra-rinsed valve. Our study suggests a platelet-injury effect by storage-solution and generates the hypothesis of a role for the biomaterial in stimulating innate-immunity. Larger prospective studies are needed. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s12265-021-10117-9.
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- 2021
171. Mechanisms by which autophagy regulates memory capacity in ageing
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Andrea Mele, Maria De Risi, Martine Ammassari-Teule, Manon Rivagorda, Annabella Pignataro, Nicolò Carrano, Silvia Middei, Giulia Torromino, Carmine Settembre, Elvira De Leonibus, Fabrizio Gardoni, Michele Tufano, Stéphanie Moriceau, Franck Oury, De Risi, M., Torromino, G., Tufano, M., Moriceau, S., Pignataro, A., Rivagorda, M., Carrano, N., Middei, S., Settembre, C., Ammassari-Teule, M., Gardoni, F., Mele, A., Oury, F., and De Leonibus, E.
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0301 basic medicine ,Agonist ,autophagy ,Aging ,Spermidine ,medicine.drug_class ,alpha-synuclein ,Biology ,amyloid fibrils ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,mild cognitive impairment ,0302 clinical medicine ,Memory ,amyloid fibril ,medicine ,Animals ,Cognitive Dysfunction ,Alpha-synuclein ,Neurodegeneration ,Autophagy ,Original Articles ,Cell Biology ,Impaired memory ,medicine.disease ,Cell biology ,Disease Models, Animal ,alpha‐synuclein ,030104 developmental biology ,chemistry ,ageing ,Ageing ,Synaptic plasticity ,Original Article ,GluA1 ,030217 neurology & neurosurgery - Abstract
Autophagy agonists have been proposed to slow down neurodegeneration. Spermidine, a polyamine that acts as an autophagy agonist, is currently under clinical trial for the treatment of age‐related memory decline. How Spermidine and other autophagy agonists regulate memory and synaptic plasticity is under investigation. We set up a novel mouse model of mild cognitive impairment (MCI), in which middle‐aged (12‐month‐old) mice exhibit impaired memory capacity, lysosomes engulfed with amyloid fibrils (β‐amyloid and α‐synuclein) and impaired task‐induced GluA1 hippocampal post‐translation modifications. Subchronic treatment with Spermidine as well as the autophagy agonist TAT‐Beclin 1 rescued memory capacity and GluA1 post‐translational modifications by favouring the autophagy/lysosomal‐mediated degradation of amyloid fibrils. These findings provide new mechanistic evidence on the therapeutic relevance of autophagy enhancers which, by improving the degradation of misfolded proteins, slow down age‐related memory decline., We developed a novel mouse model of mild cognitive impairment (MCI) that allows to identify middle‐aged (12‐month‐old) mice with impaired memory capacity and vulnerable to age‐dependent memory decline. Spermidine and TAT‐Beclin 1, by stimulating autophagy/lysosomal degradation of misfolded protein, reduce the amyloid load and rescue the memory‐dependent post‐translational modifications of GluA1 receptors in the hippocampus of the cognitively impaired ageing mice.
- Published
- 2020
172. The splicing FK506-binding protein-51 isoform plays a role in glioblastoma resistance through programmed cell death ligand-1 expression regulation
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Felix Hausch, Vincenza Vigorito, Salvatore Russo, Anna Rea, Michael Bauder, Marina Digregorio, Matthias Dedobbeleer, Paolo D'Arrigo, Martina Tufano, Maria Fiammetta Romano, Simona Romano, Bernard Rogister, D'Arrigo, P., Digregorio, M., Romano, Simona, Tufano, M., Rea, A., Hausch, F., Dedobbeleer, M., Vigorito, V., Russo, S., Bauder, M., Rogister, B., and Romano, M. F.
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0301 basic medicine ,Gene isoform ,Cancer Research ,Programmed cell death ,Immunology ,Apoptosis ,lcsh:RC254-282 ,Article ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Glioma ,medicine ,Gene silencing ,lcsh:QH573-671 ,Chemistry ,lcsh:Cytology ,Apoptosi ,Cell Biology ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Immune checkpoint ,In vitro ,Cell biology ,CNS cancer ,030104 developmental biology ,030220 oncology & carcinogenesis ,RNA splicing - Abstract
Gliomas aberrantly express programmed cell death ligand-1 (PD-L1), which has a pivotal role in immunoevasion. The splicing isoform of FKBP5, termed FKBP51s, is a PD-L1 foldase, assisting the immune checkpoint molecule in maturation and expression on the plasma membrane. The concept that PD-L1 supports tumor-intrinsic properties is increasingly emerging. The aim of the present work was to confirm the pro-tumoral effect of PD-L1 on human glioma cell survival, stemness capacity and resistance, and to address the issue of whether, by targeting its foldase either chemically or by silencing, the aggressive tumor features could be attenuated. PD-L1-depleted glioma cells have a reduced threshold for apoptosis, while PD-L1 forced expression increases resistance. Similar results were obtained with FKBP51s modulation. The ability of PD-L1 to counteract cell death was hampered by FKBP51s silencing. PD-L1 expression was particularly high in glioma cells with a cancer-stem-cell profile. Moreover, PD-L1 sustained the spheroid formation capability of glioma cells. Targeting of FKBP51s by small-interfering RNA (siRNA) or the specific inhibitor SAFit2, reduced the number of formed spheroids, along with PD-L1 expression. Finally, in an orthotopic mouse model of glioblastoma, daily treatment with SAFit2 significantly reduced tumor PD-L1 expression, and tumor growth. In treated mice, caspase-3 activation and reduced vimentin expression were observed in excised tumors. In conclusion, targeting of FKBP51s hampers PD-L1 and its pro-tumoral properties, thereby affecting the self-renewal and growth capacities of glioblastoma cells in vitro and in vivo.
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- 2019
173. Combining Magnetic Resonance Imaging with Systemic Monocyte Evaluation for the Implementation of GBM Management
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Alessandro Olivi, Simona Romano, Martina Tufano, Marco Gessi, Carolina Giordano, Quintino Giorgio D'Alessandris, Cesare Colosimo, Maria Romano, Mario Balducci, Simona Gaudino, Giuseppe Maria Della Pepa, Simone Cottonaro, Giovanni Sabatino, Giordano, C., Sabatino, G., Romano, S., Pepa, G. M. D., Tufano, M., D'Alessandris, Q. G., Cottonaro, S., Gessi, M., Balducci, M., Romano, M. F., Olivi, A., Gaudino, S., and Colosimo, C.
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Male ,Pathology ,Settore MED/27 - NEUROCHIRURGIA ,Lipopolysaccharide Receptor ,Lipopolysaccharide Receptors ,Monocyte ,B7-H1 Antigen ,Monocytes ,lcsh:Chemistry ,Immunophenotyping ,Pseudoprogression ,Receptors ,Prospective Studies ,lcsh:QH301-705.5 ,Spectroscopy ,medicine.diagnostic_test ,Brain Neoplasms ,General Medicine ,Middle Aged ,Flow Cytometry ,Magnetic Resonance Imaging ,CD ,Computer Science Applications ,medicine.anatomical_structure ,FKBP51s ,Differentiation ,Cell Surface ,Female ,Human ,MRI ,Adult ,medicine.medical_specialty ,CD14 ,Antigens, Differentiation, Myelomonocytic ,Receptors, Cell Surface ,Glioblastoma multiforme ,Real-Time Polymerase Chain Reaction ,Article ,Catalysis ,Flow cytometry ,Brain Neoplasm ,Tacrolimus Binding Proteins ,Inorganic Chemistry ,Antigens, CD ,medicine ,Humans ,Antigens ,Physical and Theoretical Chemistry ,Liquid biopsy ,Molecular Biology ,Aged ,business.industry ,Tacrolimus Binding Protein ,Organic Chemistry ,Magnetic resonance imaging ,Myelomonocytic ,Prospective Studie ,FKBP51 ,lcsh:Biology (General) ,lcsh:QD1-999 ,Glioblastoma ,business ,CD163 - Abstract
Magnetic resonance imaging (MRI) is the gold standard for glioblastoma (GBM) patient evaluation. Additional non-invasive diagnostic modalities are needed. GBM is heavily infiltrated with tumor-associated macrophages (TAMs) that can be found in peripheral blood. FKBP51s supports alternative-macrophage polarization. Herein, we assessed FKBP51s expression in circulating monocytes from 14 GBM patients. The M2 monocyte phenotype was investigated by qPCR and flow cytometry using antibodies against PD-L1, CD163, FKBP51s, and CD14. MRI assessed morphologic features of the tumors that were aligned to flow cytometry data. PD-L1 expression on circulating monocytes correlated with MRI tumor necrosis score. A wider expansion in circulating CD163/monocytes was measured. These monocytes resulted in a dramatic decrease in patients with an MRI diagnosis of complete but not partial surgical removal of the tumor. Importantly, in patients with residual tumor, most of the peripheral monocytes that in the preoperative stage were CD163/FKBP51s− had turned into CD163/FKBP51s+. After Stupp therapy, CD163/FKBP51s+ monocytes were almost absent in a case of pseudoprogression, while two patients with stable or true disease progression showed sustained levels in such circulating monocytes. Our work provides preliminary but meaningful and novel results that deserve to be confirmed in a larger patient cohort, in support of potential usefulness in GBM monitoring of CD163/FKBP51s/CD14 immunophenotype in adjunct to MRI.
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- 2021
174. Chemistry of modern paint media: The strained and collapsed painting by Alexis Harding
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Francesca Sabatini, Francesca Modugno, Maria Katia Tufano, Beatrice Campanella, Ilaria Degano, Martina Zuena, Stefano Legnaioli, J. La Nasa, Federica Nardella, Patrizia Tomasin, Luca Nodari, La Nasa, J, Nodari, L, Nardella, F, Sabatini, F, Degano, I, Modugno, F, Legnaioli, S, Campanella, B, Tufano, M, Zuena, M, and Tomasin, P
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Painting ,Organic pigment ,infrared spectroscopy ,Mass spectrometry ,Polymer science ,Synthetic resin ,010401 analytical chemistry ,Alkyd ,Analytical pyrolysis ,02 engineering and technology ,Alkyd resin ,Natural oils ,Modern-oil paint ,021001 nanoscience & nanotechnology ,01 natural sciences ,Mass spectrometric ,0104 chemical sciences ,Analytical Chemistry ,visual_art ,visual_art.visual_art_medium ,0210 nano-technology ,Spectroscopy - Abstract
The paint media used by contemporary artists include a wide range of formulation of industrially produced paints based on synthetic resins and natural oils. We investigated the paint media used by the British artist Alexis Harding (born in 1973) in the painting “Quartet” (2003). The artwork exemplifies the artist's technique of mixing different paint media to obtain a sliding movement of the paint over the support, raising severe conservation issues and causing long-term instability of the paint. As a basis to develop a conservation strategy for the artwork, we investigated the composition of the paint by in situ non-invasive contactless external reflection infrared spectroscopy (ER-FTIR). The combination with laboratory analytical techniques applied on micro-samples allowed the identification of the pigments and the binders. The multi-analytical approach involved micro infrared spectroscopy (µFTIR), Raman spectroscopy, X-ray fluorescence (XRF), and mass spectrometric techniques coupled to chromatography (GC/MS and HPLC-ESI-Q-ToF) and analytical pyrolysis (Py-GC/MS and EGA-MS). The results of these micro-destructive investigations showed that the causes of the gliding of the paint are due the incompatibility between different materials superimposed by the artist: a fast drying alkyd paint and a mixture of siccative and non-siccative drying oils.
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- 2020
175. FKBP (FK506 Binding Protein)
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Paolo D’Arrigo, Martina Tufano, Anna Rea, Simona Romano, Maria Fiammetta Romano, D'Arrigo P, Tufano M, Rea A, Romano S, Romano MF., Sangdun Choi, D'Arrigo, Paolo, Tufano, Martina, Rea, Anna, Romano, Simona, and Romano, MARIA FIAMMETTA
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FKBP, classification, gene, structure, function - Abstract
In the 70s, after a decade from the purification of cyclosporine, a selective immunosuppressant agent and potent tool in transplantation medicine, a novel molecule was purified from bacteria Streptomyces tsukubaensis. This molecule, called FK506, showed the same selective immunosuppressant action as cyclosporine but was 10 to 100 fold more potent. In an attempt to clarify the molecular mechanism through which the new drug exerted such a selective effect on T-cells activation, two laboratories identified the cytosolic receptor for FK506. This so-called FK506 binding protein (FKBP) was purified from bovine thymus, human spleen, and Jurkat T-cell line. The isolated FKBP had an approximate molecular mass of 14 kDa and showed an isomerase activity similar to the recently purified cyclosporine-binding protein, cyclophilin, but, it was inhibited by FK506 and rapamycin but not cyclosporine. The subsequent cloning of FKBP gene revealed that FKBP and cyclophilin had dissimilar sequences in spite of their common enzymatic activity. The identified FKBP gene encoded for a protein of 108 aminoacids with a relative molecular mass of 11,819. For this reason, the progenitor of this nascent class of proteins was later known as FKBP12. The subsequent studies showed that FKBP12 was just a member of a ubiquitous and evolutionarily conserved sub-family of proteins which differ from each other in their molecular weight and structure. All FKBPs share a highly conserved domain, termed “FK-12 like domain”, capable of binding to FK506 and exerting isomerase properties, i.e. interconversion from cis-to-trans and trans-to-cis of peptide bonds involving proline, on protein substrates. A schematic historical background of the 17 FKBPs so far identified is shown. A general overview of FKBP structure, function and eventually associated disease is given in this monograph, with the order of proteins following the chronology of discovery.
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- 2016
176. Identification of a highly suppressive Treg subset associated to immunotherapy response
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Martina Tufano, Simona Romano, Emilio Francesco Giunta, Maria Romano, D. Faicchia, Giuseppe Argenziano, Fortunato Ciardiello, N. Novizio, P. D'Arrigo, G. Matarese, Teresa Troiani, A. Rea, Vincenza Vigorito, C. Procaccini, V. De Falco, Giunta, Ef, Romano, Simona, D’Arrigo, P, Rea, A, Tufano, M, Matarese, G, Procaccini, C, Novizio, N, Vigorito, V, Faicchia, D, Argenziano, G, De Falco, V, Ciardiello, F, Romano, Mf, and Troiani, T
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Oncology ,business.industry ,medicine.medical_treatment ,Immunology ,medicine ,Identification (biology) ,Hematology ,Immunotherapy ,business - Published
- 2018
177. Effect of Temperature on the Shift of Pseudomonas Fluorescens from an Environmental Microorganism to a Potential Human Pathogen
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Maria Antonietta Tufano, Alessandra Fusco, Elisabetta Buommino, Iole Paoletti, Lucia Auricchio, Giovanna Donnarumma, Donnarumma, Giovanna, Buommino, Elisabetta, Fusco, A., Paoletti, I., Auricchio, L., Tufano, M. A., Donnarumma, G, Fusco, A, Paoletti, I, Auricchio, L, and Tufano, Ma
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beta-Defensins ,Microorganism ,Immunology ,Virulence ,Pseudomonas fluorescens ,Human pathogen ,Bacterial Adhesion ,Cell Line ,Proinflammatory cytokine ,Microbiology ,Humans ,Immunology and Allergy ,Psychrophile ,Pharmacology ,biology ,Chemistry ,Pseudomonas fluorescen ,Temperature ,Biofilm ,Intercellular Adhesion Molecule-1 ,biology.organism_classification ,adhesion ,Biofilms ,proinflammatory cytokines ,Cytokines ,Bacteria - Abstract
Pseudomonas fluorescens is a Gram-negative bacterium generally considered of scarce clinical significance. However, in the last few years, the isolation of P. fluorescens as the causative agent of nosocomial infections has rapidly increased. P. fluorescens is a psychrophile microorganism which grows at an optimal temperature of 25-30 degrees Celcius. In spite of this constraint, it has recently been reported that the human physiological temperature does not appear to be a barrier for this microorganism. In this study we examined the ability of P. fluorescens, grown at 28 degrees C or at 37 degrees C, to adhere to cultured human A549 pulmonary cells and to form biofilm. The ability of P. fluorescens to induce expression of proinflammatory cytokines, beta-defensin 2 and the intercellular adhesion molecule-1 was also investigated. Our results clearly indicate that inflammatory mediators are induced when the microorganism is grown at a lower temperature, while biofilm is formed only at 37 degrees C. The results presented are consistent with previous reports indicating P. fluorescens as an opportunistic pathogen and underscore the urgent need for further studies to better characterize the virulence of this microorganism.
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- 2010
178. Antibacterial Activity of Cefditoren Against Major Community-Acquired Respiratory Pathogens Recently Isolated in Italy
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Roberto Mattina, Stefania Stefani, M.A. Tufano, Fabio Rossano, Giorgio Palù, Guido Fadda, G. C. Schito, Giuseppe Nicoletti, Maria Lina Mezzatesta, Stefani, S., Mezzatesta, M., Fadda, G., Mattina, R., Palu, G., Rossano, Fabio, Tufano, M., Schito, G., and Nicoletti, G.
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Streptococcus pyogenes ,medicine.drug_class ,Antibiotics ,Cephalosporin ,Microbial Sensitivity Tests ,medicine.disease_cause ,Microbiology ,Haemophilus influenzae ,Amp resistance ,Levofloxacin ,polycyclic compounds ,Humans ,Medicine ,Pharmacology (medical) ,Respiratory Tract Infections ,Pharmacology ,SEQUENCES ,Respiratory tract infections ,business.industry ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Anti-Bacterial Agents ,Cephalosporins ,Community-Acquired Infections ,Klebsiella pneumoniae ,Streptococcus pneumoniae ,Infectious Diseases ,Italy ,Oncology ,bacteria ,business ,Moraxella catarrhalis ,Cefditoren ,medicine.drug - Abstract
In this study we evaluated the in vitro activities of cefditoren--a broad-spectrum oral cephalosporin--and other comparator agents against 2,396 fresh isolates from community-acquired respiratory tract infections, collected from 6 clinical Italian microbiology laboratories. On penicillin-susceptible pneumococci and Streptococcus pyogenes, cefditoren demonstrated to be the most active antibiotic (MIC(90)values of 0.03 and 0.06 mg/L respectively), showing only a slight decrease in potency on penicillin-intermediate and resistant pneumococci (MIC(90)value 0.5 mg/L, 1.0 mg/L respectively). All the other comparators displayed MIC(90 )values of 4 - 8 mg/L for penicillins and of 4 to64 mg/L for the oral cephalosporins. Cefditoren and levofloxacin were the most active against MSSA (MIC(90)0.5 mg/mL). Cefditoren displayed a uniformly potent inhibitory activity (MIC(90)of 0.03 mg/L) against all strains of Haemophilus influenzae, regardless of their ampicillin resistance (mediated or not by beta-lactamase production), while against Moraxella catarrhalis MIC(90)values were higher against beta-lactamase-positive (0.25 mg/L). Cefditoren was active also against Klebsiella pneumoniae and Escherichia coli : in this case its activity was comparable with that of levofloxacin. In conclusion, cefditoren, due to its potent activity, is a new effective therapeutic option for the treatment of respiratory tract infections.
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- 2008
179. Anti-inflammatory effects of moxifloxacin and human β-defensin 2 association in human lung epithelial cell line (A549) stimulated with lipopolysaccharide
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Iole Paoletti, Maria Rosaria Iovene, Elisabetta Buommino, Laura Tudisco, Maria Antonietta Tufano, Giovanna Donnarumma, Valentina Cozza, Donnarumma, G, Paoletti, I, Buommino, Elisabetta, Iovene, Mr, Tudisco, L, Cozza, V, Tufano, Ma, Donnarumma, Giovanna, Paoletti, I., Iovene, Maria Rosaria, Tudisco, L., Cozza, V., and Tufano, M. A.
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Lipopolysaccharides ,beta-Defensins ,Gram-negative bacteria ,Lipopolysaccharide ,Neutrophils ,Physiology ,Interleukin-1beta ,Moxifloxacin ,Antimicrobial peptides ,Gene Expression ,Biochemistry ,Cell Line ,Microbiology ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Endocrinology ,Cell Adhesion ,medicine ,Humans ,A549 cells ,Lung ,Defensin ,Inflammation ,A549 cell ,Aza Compounds ,biology ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Anti-Inflammatory Agents, Non-Steroidal ,Interleukin-8 ,Epithelial Cells ,beta-Defensin 2 ,Intercellular Adhesion Molecule-1 ,biology.organism_classification ,Antimicrobial ,medicine.anatomical_structure ,chemistry ,Quinolines ,Cytokines ,Fluoroquinolones ,medicine.drug ,Respiratory tract - Abstract
Epithelia in the human airways, from the nasal aperture to the alveoli, are covered in a protective film of fluid containing a number of antimicrobial proteins. Defensins are single-chain, strongly cationic peptides and are one of the most extensively studied classes of antimicrobial peptides. Moxifloxacin (MXF) is a fluoroquinolone that acts against both Gram positive and Gram negative bacteria. In this study, we evaluated the effects of HBD2, MXF and the association MXF/HBD2 on some cytokines and on the ICAM-1 expression in LPS-stimulated A549 cells. Our results suggest that by lowering the epithelial cell-derived IL-1beta, IL-6, IL-8 and ICAM-1 expression, the MXF/HBD2 association interferes with the multifunctional cytokine network evolving during inflammatory processes of the respiratory tract; this anti-inflammatory potential could be of great value in the treatment of inflammatory disorders.
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- 2007
180. Bacterial and Viral Skin Diseases
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Maria Antonietta Tufano, Eleonora Ruocco, Giovanna Donnarumma, Adone Baroni, Ruocco, Eleonora, Donnarumma, Giovanna, Baroni, Adone, and Tufano, M. A.
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integumentary system ,business.industry ,Secondary infection ,Healthy subjects ,Skin Diseases, Bacterial ,Dermatology ,Skin infection ,medicine.disease ,Antiviral Agents ,Viral skin diseases ,Anti-Bacterial Agents ,Microbiology ,Immune system ,Bacterial Skin Disease ,Flora (microbiology) ,Skin Diseases, Viral ,Immunology ,Humans ,Medicine ,Colonization ,Viral disease ,business ,Viral Skin Diseases - Abstract
At least two populations of microorganisms are found in skin microbiota: a resident flora and a transient flora. Colonization and invasion by pathogenous microorganisms is counteracted both by the host defenses and by the resident flora. Most skin infections are therefore self-limiting in healthy subjects and are defined as primary infections. Secondary infections develop on preexisting skin lesions and are usually polymicrobial and caused by microorganisms that in themselves have little pathogenic power. When immune defenses are low, secondary infections arise readily and develop rapidly. This article describes the main bacterial and viral skin diseases.
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- 2007
181. Antiviral effects of quinine sulfate on HSV-1 HaCat cells infected: Analysis of the molecular mechanisms involved
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Federica Corrado, Giovanna Donnarumma, Iole Paoletti, Eleonora Ruocco, Fabio Ayala, Adone Baroni, Maria Antonietta Tufano, Ronni Wolf, Baroni, Adone, Paoletti, I., Ruocco, Eleonora, Ayala, F., Corrado, F., Wolf, R., Tufano, M., and Donnarumma, Giovanna
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Herpesvirus 1, Human ,Dermatology ,Virus Replication ,Antiviral Agents ,Biochemistry ,Immediate early protein ,Immediate-Early Proteins ,Antimalarials ,Cell Line, Tumor ,Chlorocebus aethiops ,Animals ,Humans ,Medicine ,HSP70 Heat-Shock Proteins ,Vero Cells ,Molecular Biology ,Herpes simplex virus protein vmw65 ,Quinine ,business.industry ,NF-kappa B ,Herpes Simplex Virus Protein Vmw65 ,HSV-1, Quinine sulfate, Hsp70, HaCat ,Virology ,Hsp70 ,HaCaT ,Viral replication ,Vero cell ,Quinine Sulfate ,business ,medicine.drug - Abstract
Quinine sulfate (QS), the first antimalarial agent, was derived from the cinchona tree. Interestingly, antimalarials were recently found effective as additional therapy for AIDS and other viral diseases. The antiviral action of some drugs is associated with the induction of heat shock protein synthesis, in particular of the 70 kDa heat shock protein (Hsp70). In the current study, we examined the effects of QS on HSV-1 replication in HaCat cells. We also estimated the effects of this drug on the Hsp70, ICP27 and VP16 production and on the activation of NF-kB in HaCat cells that are HSV-1 infected to elucidate the possible mechanisms of the antiviral action of QS.
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- 2007
182. Constitutional 11q14-q22 chromosome deletion syndrome in a child with neuroblastoma MYCN single copy
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Gian Paolo Tonini, Maria Capasso, Raffaella Defferrari, Maria Antonietta Tufano, Lucio Nitsch, Tommy Martinsson, Katia Mazzocco, Annalisa Passariello, Roberta Migliorati, Daniele De Brasi, Paolo Siani, Rita Genesio, Passariello, Annalisa, De Brasi, D, Defferrari, R, Genesio, R, Tufano, M, Mazzocco, K, Capasso, M, Migliorati, Roberta, Martinsson, T, Siani, P, Nitsch, Lucio, and Tonini, Gp
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SLE ,domain containing 5 ,Gene Dosage ,European cytogeneticists association register of unbalanced chromosome aberration ,Germline ,Loss of heterozygosity ,Neuroblastoma ,pseudo autosomal region 1 ,systemic lupus erythematosus ,MYCN ,DECIPHER ,Jacobsen syndrome ,multiplex ligation-dependent probe amplification ,11q syndrome ,Genetics (clinical) ,Genetics ,Oncogene Proteins ,N-Myc Proto-Oncogene Protein ,123-iodine metaidobenzoguadinyl scintigrafy ,database of genomic variant ,MMP ,medicine.diagnostic_test ,Brain Neoplasms ,MMP13 ,Nuclear Proteins ,General Medicine ,Syndrome ,matrix metalloproteinase gene ,DGV ,PAR1 ,MLPA ,International NB Staging System ,structural copy number variation ,ECARUCA ,GYG2 ,array comparative genomic hybridization ,Female ,INSS ,ARSD ,matrix metalloproteinase 13 ,Chromosome Deletion ,ARSE ,CR ,ARSH ,DCUN1D5 ,CNV ,Trigonocephaly ,Biology ,Craniosynostoses ,FISH ,aCGH ,Hypotelorism ,critical region ,medicine ,MCA/MR ,Humans ,Abnormalities, Multiple ,DCN1 ,Multiplex ligation-dependent probe amplification ,fluorescence in situ hybridization ,Oncogene ,Germ-Line Mutation ,Chromosomes, Human, Pair 11 ,database of chromosomal imbalance and phenotype in humans using ensembl resource ,Infant ,arilsulphatases gene ,multiple congenital anomaly/mental retardation ,medicine.disease ,(123)MIBG ,defective in cullin neddylation 1 ,Cancer research ,NB ,glycogenin 2 gene ,Fluorescence in situ hybridization - Abstract
Constitutional 11q deletion is a chromosome imbalance possibly found in MCA/MR patients analyzed for chromosomal anomalies. Its role in determining the phenotype depends on extension and position of deleted region. Loss of heterozygosity of 11q (region 11q23) is also associated with neuroblastoma, the most frequent extra cranial cancer in children. It represents one of the most frequent cytogenetic abnormalities observed in the tumor of patients with high-risk disease even if germline deletion of 11q in neuroblastoma is rare. Hereby, we describe a 18 months old girl presenting with trigonocephaly and dysmorphic facial features, including hypotelorism, broad depressed nasal bridge, micrognathia, synophrys, epicanthal folds, and with a stage 4 neuroblastoma without MYCN amplification, carrying a germline 11q deletion (11q14.1-q22.3), outside from Jacobsen syndrome and from neuroblastoma 11q critical regions. The role of 11q deletion in determining the clinical phenotype and its association with neuroblastoma development in the patient are discussed.
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- 2013
183. INNATE IMMUNE RESPONSE IN HUMAN KERATINOCYTES INFECTED BY A FELINE ISOLATE OF MALASSEZIA PACHYDERMATIS
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BUOMMINO, Elisabetta, DONNARUMMA, Giovanna, De Filippis A, Parisi A, Nizza S, Martano M, Iovane G, Tufano MA, De Martino L., DE FILIPPIS, Anna, Buommino, Elisabetta, De Filippis, A, Parisi, A, Nizza, S, Martano, M, Iovane, G, Donnarumma, Giovanna, Tufano, Ma, De Martino, L., DE FILIPPIS, Anna, De Filippis, A., Parisi, A., Nizza, Sandra, Martano, Manuela, Iovane, Giuseppe, Donnarumma, G., Tufano, M. A., and DE MARTINO, Luisa
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Keratinocytes ,Innate immune response ,beta-Defensins ,Human keratinocyte ,cat ,Biology ,Real-Time Polymerase Chain Reaction ,Microbiology ,Cell Line ,Proinflammatory and immunomodulatory cytokines ,Immunity ,Animals ,Dermatomycoses ,Humans ,Regulation of gene expression ,Malassezia ,Innate immune system ,General Veterinary ,Epidermis (botany) ,Malassezia pachydermati ,General Medicine ,Immunity, Innate ,Toll-Like Receptor 2 ,Malassezia pachydermatis ,HaCaT ,TLR2 ,Gene Expression Regulation ,Cell culture ,Immunology ,Cats ,Cytokines - Abstract
Malassezia pachydermatis is a normal inhabitant of canine and feline skin that can spread to other pets. The outer layer or epidermis is made up primarily of keratinocytes, which are capable of releasing various factors and expressing receptors that are significantly involved in the immune regulation. Little is known about the mechanism by which M. pachydermatis overcomes the natural barrier of the skin. The aim of this study was to evaluate the direct in vitro interaction between human keratinocytes and a clinical strain of live M. pachydermatis isolated as a pure culture from an otitic cat. Human keratinocytes (HaCat) were infected with M. pachydermatis to analyse the modulation of the innate immune response. Gene expression was analysed by real-time PCR. We demonstrated that M. pachydermatis invaded HaCat cells and modulated the expression of TLR2 after 24. h infection, while HBD-2, IL-1β TNF-α, IL-6 and IL-8 were modulated both at 24 and 48. h. Thus, our results demonstrated that M. pachydermatis is able to stimulate the innate immune response in infected human keratinocytes indicating a possible role of this yeast as a human opportunistic pathogen.
- Published
- 2013
184. Design of inhibitors of influenza virus membrane fusion: Synthesis, structure-activity relationship and in vitro antiviral activity of a novel indole series
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Alan J. Hay, Mario De Rosa, Antonella Peduto, Chiara Schiraldi, Brunella Perfetto, Giovanna Donnarumma, Antonio Massa, Stephen R. Martin, Antonia Di Mola, Virginia Brancato, Rosanna Filosa, Maria Antonietta Tufano, Vijaykumar More, Stephen A. Wharton, Brancato, V, Peduto, A, Wharton, S, Martin, S, More, V, Di Mola, A, Massa, A, Perfetto, B, Donnarumma, G, Schiraldi, C, Tufano, M, de Rosa, M, Filosa, R, Hay, A, Perfetto, Brunella, Donnarumma, Giovanna, Schiraldi, Chiara, Tufano, Ma, DE ROSA, Mario, Filosa, Rosanna, and Hay, A.
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Conformational change ,Arbidol derivatives ,Indoles ,Endosome ,Influenza haemagglutinin ,Hemagglutinin Glycoproteins, Influenza Virus ,CHO Cells ,Biology ,Antiviral Agents ,Membrane Fusion ,Virus ,Cell Line ,Madin Darby Canine Kidney Cells ,Fluorescence binding assay ,chemistry.chemical_compound ,Structure-Activity Relationship ,Cricetulus ,Dogs ,Virology ,Cricetinae ,Influenza, Human ,Structure–activity relationship ,Animals ,Humans ,Hydroxymethyl ,Pharmacology ,Indole test ,Arbidol derivative ,Lipid bilayer fusion ,Virus Internalization ,Antiviral action, Arbidol derivatives, Differential binding to haemagglutinin, Fluorescence binding assay, Influenza haemagglutinin, PH stabilization ,In vitro ,chemistry ,Biochemistry ,Influenza A virus ,Drug Design ,PH stabilization ,Differential binding to haemagglutinin ,Antiviral action - Abstract
The fusion of virus and endosome membranes is an essential early stage in influenza virus infection. The low pH-induced conformational change which promotes the fusogenic activity of the haemagglutinin (HA) is thus an attractive target as an antiviral strategy. The anti-influenza drug Arbidol is representative of a class of antivirals which inhibits HA-mediated membrane fusion by increasing the acid stability of the HA. In this study two series of indole derivatives structurally related to Arbidol were designed and synthesized to further probe the foundation of its antiviral activity and develop the basis for a structure-activity relationship (SAR). Ethyl 5-(hydroxymethyl)-1-methyl-2-(phenysulphanylmethyl)-1. H-indole-3-carboxylate (15) was identified as one of the most potent inhibitors and more potent than Arbidol against certain subtypes of influenza A viruses. In particular, 15 exhibited a much greater affinity and preference for binding group 2 than group 1 HAs, and exerted a greater stabilising effect, in contrast to Arbidol. The results provide the basis for more detailed SAR studies of Arbidol binding to HA; however, the greater affinity for binding HA was not reflected in a comparable increase in antiviral activity of 15, apparently reflecting the complex nature of the antiviral activity of Arbidol and its derivatives.
- Published
- 2013
185. Cell-growth and migration inhibition of human mesothelioma cells induced by 3-O-Methylfunicone from Penicillium pinophilum and cisplatin
- Author
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BUOMMINO, Elisabetta, RIZZO, Antonietta, DONNARUMMA, Giovanna, A. DE FILIPPIS, R. NICOLETTI, M. MENEGOZZO, S. MENEGOZZO, M. L. CIAVATTA, V. BRANCATO, M. A. TUFANO, DE FILIPPIS, Anna, Buommino, Elisabetta, A, DE FILIPPIS, R, Nicoletti, M, Menegozzo, S, Menegozzo, M, L, A, Rizzo, V, Brancato, M, A, Donnarumma, G., A., DE FILIPPIS, R., Nicoletti, M., Menegozzo, S., Menegozzo, M. L., Ciavatta, Rizzo, Antonietta, V., Brancato, M. A., Tufano, Donnarumma, Giovanna, DE FILIPPIS, Anna, Buommino, E, De Filippis, A, Nicoletti, R, Menegozzo, M, Menegozzo, S, Ciavatta, M, Rizzo, A, Brancato, V, Tufano, M, and Donnarumma, G
- Subjects
inorganic chemicals ,Oncology ,Mesothelioma ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Angiogenesis ,Motility ,Integrin ,Drug resistance ,Biology ,Cell Movement ,Internal medicine ,Cell Line, Tumor ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,3-O-methylfunicone ,Humans ,Pharmacology (medical) ,Receptors, Vitronectin ,RNA, Messenger ,neoplasms ,Cell Shape ,Metalloproteinase ,Migration ,Cell Proliferation ,Pharmacology ,Cisplatin ,Cell growth ,Chemotaxis ,Penicillium ,medicine.disease ,In vitro ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,Cell culture ,Pyrones ,Cancer research ,Matrix Metalloproteinase 2 ,Human ,medicine.drug - Abstract
Malignant pleural mesothelioma is a fatal malignancy linked to asbestos exposure. The main challenge for mesothelioma treatment is to go beyond the drug resistance, in particular against cisplatin (CDDP), one of the most used chemotherapeutic drug. 3-O-methylfunicone (OMF) is a metabolite produced by the fungus Penicillium pinophilum; its antiproliferative properties have been previously studied in vitro. Particularly, OMF is able to inhibit mesothelioma cell motility. To improve the effects of CDDP by-passing the resistance of mesothelioma cells to this drug, in the present study we investigated the combined treatment of OMF with CDDP respectively in an established mesothelioma cell line (NCI) and primary mesothelioma cells (Mest). As compared to the effect of single treatments, the combination of OMF and CDDP resulted in a stronger inhibition of NCI and Mest cell proliferation. OMF combination with CDDP was also able to affect the migratory ability of NCI and Mest cells by down-regulating αv and β5 expression and reducing metalloproteinase 2 (MMP-2) production. In addition, this association was effective in modulating VEGF gene expression. This finding highlights the possibility to use OMF and CDDP together to regulate angiogenesis and tumour progression in mesothelioma.
- Published
- 2012
186. The Helicobacter pylori protein HspB interferes with Nrf2/Keap-1 pathway altering the antioxidant response of ags cells
- Author
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BUOMMINO, Elisabetta, DONNARUMMA, Giovanna, DE LUCA, Antonio, Manente L., De Filippis A., Francesco Silvestri F., Iaquinto S., Tufano M.A., DE FILIPPIS, Anna, Buommino, Elisabetta, Donnarumma, Giovanna, Manente, L., De Filippis, A., Francesco Silvestri, F., Iaquinto, S., Tufano, M. A., DE LUCA, Antonio, and DE FILIPPIS, Anna
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CagA ,HspB ,Helicobacter pylori ,VacA ,Keap1 pathway ,Nrf2 - Abstract
Background Helicobacter pylori infection causes chronic oxidative stress on gastric mucosa, thereby causing mucosal damage and increasing the risk of gastric adenocarcinoma. Nrf2 is an important transcription factor, regulating the antioxidant response in the cells. Nrf2 signaling is repressed by Keap1 at basal condition and induced by oxidative stress. The aim of our study was to analyze whether the H. pylori proteins interfered in the Nrf2/Keap1 pathway. Material and Methods Gene expression in AGS cells transiently and stably transfected was analyzed by real-time PCR. Immunoprecipitation and immunofluorescence assays were performed to investigate the ability of H. pylori proteins to interfere with the Nrf2 pathway. Results We demonstrated that the H. pylori HspB protein interferes with Nrf2/Keap1 pathway. When HspB was transiently transfected in AGS cells, a significant increase in Keap1 gene expression was induced. The same result was observed when AGS cells were HspB stably transfected. In this case, the increase in Keap1 was associated with reduced gene expression of Nrf2, and of the antioxidant enzymes superoxide dismutase, hemeoxygenase-1, and phase II detoxifying enzyme NAD(P)H:quinone oxidoreductase-1. Immunoprecipitation and immunofluorescence assays confirmed the ability of HspB protein to interfere with the Nrf2 pathway. Lastly, in HspB-transfected AGS cells, sustained activation of IL-8, COX2, MMP3, and MMP7 was demonstrated. Conclusion The results here reported suggest that inhibited nuclear translocation of Nrf2, associated with induced inflammation and increased production of MMPs, might represent a condition enhancing the risk of gastric adenocarcinoma.
- Published
- 2012
187. Patenting Penicillium Strains
- Author
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Elisabetta Buommino, Maria Antonietta Tufano, Rosario Nicoletti, Nicoletti, R, Buommino, Elisabetta, Tufano, Ma, and Tufano, M.
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biology ,Drug Industry ,business.industry ,Penicillium ,Bioengineering ,Industrial microbiology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Biotechnology ,Patents as Topic ,Industrial Microbiology ,Bioremediation ,Biodegradation, Environmental ,Pharmaceutical Preparations ,Agriculture ,Biofuels ,business ,Drug industry ,Penicillium species - Abstract
Penicillium species are a widespread source of biologically active compounds and enzymes which are exploited in biotechnologies. The ongoing discovery of new species, their biochemical and molecular characterization, and the application of the new findings in diverse industrial processes stimulate an increasing interest of patentees worldwide. An overview of the patents released in the last four years in agriculture, bioremediation, and in several industrial fields for the production of biofuels, food, cosmetics and pharmaceuticals is proposed for an exhaustive appreciation of the potential cues offered to inventors by these fungi.
- Published
- 2012
188. Patented natural avocado sugar modulates the HBD-2 and HBD-3 expression in human keratinocytes through toll-like receptor-2 and ERK/MAPK activation
- Author
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Elisabetta Buommino, Iole Paoletti, Giovanna Donnarumma, Philippe Msika, Alessandra Fusco, Adone Baroni, Maria Antonietta Tufano, Caroline Baudouin, Paoletti, I, Buommino, Elisabetta, Fusco, A, Baudouin, C, Msika, P, Tufano, Ma, Baroni, Adone, Donnarumma, Giovanna, Tufano, M, Baroni, A, and Donnarumma, G.
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MAPK/ERK pathway ,Keratinocytes ,beta-Defensins ,MAP Kinase Signaling System ,Antimicrobial peptides ,Carbohydrates ,Dermatology ,Biology ,Immunomodulation ,Downregulation and upregulation ,Gene expression ,Humans ,Secretion ,TLRs ,Phosphorylation ,Receptor ,Extracellular Signal-Regulated MAP Kinases ,Cells, Cultured ,Toll-like receptor ,Persea ,General Medicine ,beta-defensin ,MAPK ,Toll-Like Receptor 2 ,Cell biology ,Up-Regulation ,AV119 ,Beta defensin ,Sugars ,Keratinocyte - Abstract
Keratinocytes stimulated by microbial organisms secrete not only a variety of cytokines, chemokines and growth factors, but also antimicrobial peptides such as beta-defensins (HBDs) such as HBD-2 and HBD-3. AV119, a patented blend of avocado sugar, triggers the up-regulation of HBD-2 in skin epithelia upon contact with AV119, but the signalling mechanisms involved are not completely understood. The purpose of this study was to determine if AV119 was able to induce also the expression of HBD-3 in human keratinocytes. In addition, the receptor and intracellular pathways involved in the AV119 up-regulation of HBD-2 and HBD-3 were investigated. Our results demonstrated that AV119 induces a significantly increase of the expression of HBD-3. In addition, the HBD-2 and HBD-3 AV119-induced gene expression and release are TLR-2 dependent. Finally, we demonstrated that AV119 induced ERK/MAPK phosphorylation in human keratinocytes, thus providing evidence that HBD-2 and HBD-3 secretion is through the same transductional pathway. The ability of AV119 to induce also HBD-3 may amplify its therapeutic potential against a broader spectrum of bacterial and yeast strains responsible for human skin disorders.
- Published
- 2011
189. AV 119, a natural sugar from avocado gratissima, modulates the LPS-induced proinflammatory response in human keratinocytes
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Elisabetta Buommino, Caroline Baudouin, Maria Antonietta Tufano, Alessandra Fusco, Iole Paoletti, Adone Baroni, Philippe Msika, Giovanna Donnarumma, Donnarumma, Giovanna, Paoletti, I, Buommino, Elisabetta, Fusco, A, Baudouin, C, Msika, P, Tufano, Ma, Baroni, Adone, Donnarumma, G, Tufano, M, and Baroni, A.
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Keratinocytes ,Lipopolysaccharides ,Chemokine ,LPS ,Lipopolysaccharide ,Immunology ,Carbohydrates ,Inflammation ,keratinocyte ,Proinflammatory cytokine ,Microbiology ,chemistry.chemical_compound ,Immune system ,Interleukin 20 ,medicine ,cytokine ,Humans ,Immunology and Allergy ,Receptor ,Heat-Shock Proteins ,Innate immune system ,biology ,Persea ,NF-kappa B ,TLR-4 ,Immunity, Innate ,Toll-Like Receptor 4 ,AV119 ,chemistry ,biology.protein ,Cytokines ,medicine.symptom ,Sugars - Abstract
Keratinocytes play an active role in innate immune responses by secreting a variety of cytokines and chemokines. The release of critical proinflammatory cytokines, which are necessary to activate the immune response, is induced by the stimulation of Toll-like receptors (TLRs) by molecules present on pathogenic micro-organisms such as lipopolysaccharide (LPS). AV119, a patented blend of avocado sugars, induced the aggregation of Malassezia furfur, a dimorphic, lipid-dependent yeast that is part of the normal human cutaneous commensal flora and inhibited its penetration into the keratinocytes. In the present study, the anti-inflammatory effects of AV119 were investigated in LPS-induced inflammation of human keratinocytes. In particular, we analysed the modulation of the LPS-induced expression of proinflammatory cytokines and heat shock protein 70 (HSP70) by AV119 and the involvement of TLR-4. Our data show that AV119 is able to modulate significantly the proinflammatory response in human keratinocytes, blocking the NF-kB activation in human keratinocytes.
- Published
- 2011
190. 3-O-methylfunicone, from Penicillium pinophilum, is a selective inhibitor of breast cancer stem cells
- Author
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BUOMMINO, Elisabetta, TIRINO, Virginia, De Filippis A, Silvestri F, Nicoletti R, Ciavatta ML, Pirozzi G, Tufano M.A., DE FILIPPIS, Anna, Buommino, Elisabetta, Tirino, Virginia, De Filippis, A, Silvestri, F, Nicoletti, R, Ciavatta, Ml, Pirozzi, G, Tufano, M. A., and DE FILIPPIS, Anna
- Published
- 2011
191. Aurantoside J: a New Tetramic Acid Glycoside from Theonella swinhoei. Insights into the Antifungal Potential of Aurantosides
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Rihab F. Angawi, G. Bavestrello, Maria Antonietta Tufano, Elena Grimaldi, B. Calcinai, Henny Adeleida Dien, Ernesto Fattorusso, Giuseppina Chianese, Giovanna Donnarumma, Orazio Taglialatela-Scafati, Iole Paoletti, Angawi, R. F., Bavestrello, G., Calcinai, B., Dien, H. A., Donnarumma, G., Tufano, M. A., Paoletti, I., Grimaldi, E., Chianese, Giuseppina, Fattorusso, Ernesto, TAGLIALATELA SCAFATI, Orazio, Bavestrello, G, Donnarumma, Giovanna, Chianese, G., Fattorusso, E., and Taglialatela Scafati, O.
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Antifungal ,Fusarium ,Antifungal Agents ,Magnetic Resonance Spectroscopy ,Aurantoside J ,medicine.drug_class ,Stereochemistry ,Metabolite ,Pharmaceutical Science ,Pentose ,Biology ,010402 general chemistry ,01 natural sciences ,Article ,Theonella swhinoei ,aurantosides ,Structure-Activity Relationship ,chemistry.chemical_compound ,Drug Discovery ,medicine ,Animals ,Structure–activity relationship ,Glycosides ,lcsh:QH301-705.5 ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,chemistry.chemical_classification ,Microbial Viability ,010405 organic chemistry ,antifungal activity ,Glycoside ,Theonella swinhoei ,biology.organism_classification ,3. Good health ,0104 chemical sciences ,lcsh:Biology (General) ,chemistry ,Biochemistry ,N-glycosides ,Theonella ,aurantoside - Abstract
The chemical investigation of an Indonesian specimen of Theonella swinhoei afforded four aurantosides, one of which, aurantoside J (5), is a new compound. The structure of this metabolite, exhibiting the unprecedented N-α-glycosidic linkage between the pentose and the tetramate units, has been determined through detailed spectroscopic analysis. The four obtained aurantosides have been tested against five fungal strains (four Candida and one Fusarium) responsible of invasive infections in immuno-compromised patients. The non-cytotoxic aurantoside I (4) was the single compound to show an excellent potency against all the tested strains, thus providing valuable insights about the antifungal potential of this class of compounds and the structure-activity relationships.
- Published
- 2011
192. Outer-membrane porins from Gram-negative bacteria stimulate platelet-activating-factor biosynthesis by cultured human endothelial cells
- Author
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Luigi Silvestro, Ciro Capasso, Maria Antonietta Tufano, Luigi Biancone, Fabio Rossano, Eugenio L. Iorio, Giovanni Camussi, Antonella De Martino, Adone Baroni, Tufano, M. A., Biancone, L., Rossano, Fabio, Capasso, C., Baroni, A., DE MARTINO, A., Iorio, E. L., Silvestri, L., and Camussi, G.
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Salmonella typhimurium ,biosynthesis of platelet-activating factor ,Umbilical Veins ,Gram-negative bacteria ,Porins ,Biology ,Biochemistry ,Phospholipases A ,Calcium in biology ,chemistry.chemical_compound ,Phospholipase A2 ,Acetyltransferases ,Gram-Negative Bacteria ,Extracellular ,Humans ,Platelet Activating Factor ,Cells, Cultured ,Platelet-activating factor ,biology.organism_classification ,endothelial cells ,Cell biology ,Phospholipases A2 ,Cytosol ,chemistry ,Porin ,biology.protein ,Calcium ,lipids (amino acids, peptides, and proteins) ,Endothelium, Vascular ,Bacterial outer membrane ,Bacterial Outer Membrane Proteins - Abstract
Porins are a family of hydrophobic proteins located in the outer membrane of the cell wall in Gram-negative bacteria. The effect of porins on the biosynthesis of platelet-activating factor (PAF) by cultured human umbilical-cord-vein-derived endothelial cells (HUVEC) was investigated. The results demonstrate that porins were able to induce a dose-dependent synthesis of PAF in HUVEC. PAF, synthesized after stimulation with porins, was mainly cell associated and the synthesis peaked at 15 min, decreasing rapidly thereafter. Experiments with radiolabeled precursors demonstrated that PAF, a 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, was synthesized via the remodeling pathway involving the acetylation of 1-O-alkyl-2-lyso-sn-glyceryl-3-phosphorylcholine (2-lysoPAF) generated from 1-O-alkyl-2-acyl-sn-glyceryl-3-phosphorylcholine by phospholipase-A2 activity. The activation of phospholipase A2 in HUVEC stimulated by porins was detected by observing the mobilization of [14C]arachidonic acid. In addition, the activity of acetyl-CoA:1-alkyl-sn-glycero-3-phosphorylcholine 2-O-acetyltransferase was transiently increased in porin-stimulated HUVEC and, after incubation with [3H]CoASAc or [3H]acetate, the [3H]acetyl group was incorporated into newly synthesized PAF. Porins, by forming transmembrane channels, induced a sustained influx of extracellular 45Ca2+ into the cytosol. The activation of PAF synthesis by porins depended on this influx rather than on intracellular calcium mobilization, since PAF synthesis did not occur in the absence of extracellular Ca2+.
- Published
- 1993
193. Captopril modulates acetylcholinesterase in human keratinocytes
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Maria Antonietta Tufano, Eleonora Ruocco, Vincenzo Ruocco, Valentina Cozza, R. A. Satriano, Elisabetta Buommino, Anna De Filippis, Marcella Petrazzuolo, Adone Baroni, Baroni, A, Buommino, Elisabetta, Ruocco, E, Petrazzuolo, M, DE FILIPPIS, A, Satriano, R, Ruocco, V, Cozza, V, and Tufano, M.
- Subjects
Keratinocytes ,medicine.medical_specialty ,Captopril ,Blotting, Western ,Angiotensin-Converting Enzyme Inhibitors ,Dermatology ,Cell Communication ,Cell Line ,chemistry.chemical_compound ,Acetylcholine secretion ,Internal medicine ,medicine ,Humans ,Secretion ,Cell adhesion ,Acetylcholine receptor ,Reverse Transcriptase Polymerase Chain Reaction ,General Medicine ,Acetylcholinesterase ,Acetylcholine ,Cell biology ,Up-Regulation ,HaCaT ,medicine.anatomical_structure ,Endocrinology ,Acantholysis ,Intercellular Junctions ,chemistry ,Keratinocyte ,medicine.drug - Abstract
Human keratinocytes synthesize and secrete non-neuronal acetylcholine, which acts as a local cell signaling molecule, regulating functions like proliferation, cell adhesion, motility, desmosomal cell contact, and glandular activity. The keratinocyte acetylcholine axis is composed of the enzymes mediating acetylcholine synthesis (acetyltransferase) and degradation (acetylcholinesterase), and two classes of acetylcholine receptors. In this study we investigated the effect of captopril, an ACE-inhibitor, on acetylcholinesterase and acetylcholine secretion in human keratinocytes. We analyzed the level of acetylcholinesterase in HaCat and NHEK cells by RT-PCR and Western blotting analysis. In addition, the effect of captopril on AChE activity was evaluated. We found that captopril induces a strong AChE up-regulation leading to ACh degradation and reduced secretion. Our results suggest that acantholysis induced by ACE-inhibitors might be linked to altered level of Ach.
- Published
- 2010
194. Antibiotic susceptibility of respiratory pathogens recently isolated in Italy: focus on ceftidoren
- Author
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Rosario Musumeci, R Rigoli, Ma Tufano, Clementina Cocuzza, Giuseppe Nicoletti, Viviana Cristina Tullio, La Vitali, Furneri Pm, Gianna Tempera, Manuela Prenna, Nicola Carlone, Ap Pilloni, Tempera, G, Furneri, P, Carlone, N, Cocuzza, C, Rigoli, R, Musumeci, R, Pilloni, A, Prenna, M, Tufano, M, Tullio, V, Vitali, L, and Nicoletti, G
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Cefotaxime ,Microbial Sensitivity Tests ,medicine.disease_cause ,Microbiology ,Amp resistance ,Levofloxacin ,Ampicillin ,Streptococcus pneumoniae ,Gram-Negative Bacteria ,polycyclic compounds ,medicine ,Humans ,Pharmacology (medical) ,Respiratory Tract Infections ,Pharmacology ,Respiratory tract infections ,business.industry ,biochemical phenomena, metabolism, and nutrition ,Anti-Bacterial Agents ,Cephalosporins ,Community-Acquired Infections ,Infectious Diseases ,Oncology ,Italy ,MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA ,business ,Gram-Negative Bacterial Infections ,Cefditoren ,Cefaclor ,cefditoren, antibiotic susceptibility ,medicine.drug - Abstract
The aim of this study was to evaluate the in vitro antibiotic susceptibility of respiratory pathogens recently isolated in Italy to commonly used antibiotics including cefditoren. Six clinical microbiological laboratories collected, between January and September 2009, a total of 2,510 respiratory pathogens from subjects with community-acquired respiratory tract infections (CARTI). Ceftditoren, out of all the beta-lactams studied, had the lowest MIC(90 )against 965 strains of Streptococcus pneumoniae examined, followed by cefotaxime and ceftriaxone (2% resistance in penicillin-resistant S. pneumoniae (PRSP)). Against 470 Haemophilus influenzae , independently of their production of beta-lactamases or ampicillin resistance, cefditoren was the oral cephalosporin with the best in vitro activity, comparable to that of the injectable cephalosporins and levofloxacin. Higher MIC(90)s were found for the macrolides (4 - 16 mg/l) and cefaclor (4 - 32 mg/l). As was foreseeable, Streptococcus pyogenes (225 strains) was uniformly sensitive to all the beta-lactam antibiotics, but the elevated MIC(90 )values reduced (75%) susceptibility of this pathogen to macrolides. Beta-lactamase-negative Moraxella catarrhalis (100 strains) had reduced susceptibility only to the macrolides, while the 250 beta-lactamase-producing strains also had reduced susceptibility to cefuroxime. Levofloxacin showed the lowest MIC(50)/MIC(90 )values in the producing strains, whereas cefditoren, cefotaxime and ceftriaxone in the non-producers. As regards the enterobacteriaceae, cefditoren and levofloxacin had the lowest MIC(90)s against Klebsiella pneumoniae. Cefditoren and the third-generation injectable cephalosporins had the lowest MIC(90)s against Escherichia coli (100% susceptibility) while levofloxacin was less active (86% susceptibility).In conclusion, cefditoren's wide spectrum and high intrinsic activity, as well as its capacity to overcome most of the resistance that has become consolidated in some classes of antibiotics widely used as empiric therapy for CARTI, allows us to suggest that cefditoren might be included in the european guidelines as one of the first-choice antibiotics in the treatment of CARTI.
- Published
- 2010
195. Methicillin-resistant staphylococci isolated from healthy horses and horse personnel in Italy
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Karina Mallardo, B. Facello, P Catalanotti, Ugo Pagnini, Luisa De Martino, Maria Antonietta Tufano, Maria Lucido, Giuseppe Iovane, Michelina Mallardo, DE MARTINO, Luisa, Lucido, M, Mallardo, K, Facello, B, Mallardo, M, Iovane, Giuseppe, Pagnini, Ugo, Tufano, Ma, Catalanotti, P., DE MARTINO, L., Lucido, M., Mallardo, K., Facello, B., Mallardo, M., Iovane, G., Pagnini, U., Tufano, M. A., and Catalanotti, Piergiorgio
- Subjects
Male ,Methicillin-Resistant Staphylococcus aureus ,Meticillin ,Staphylococcus pseudintermedius ,methicillin-resistant staphylococci ,Drug resistance ,Nose ,medicine.disease_cause ,Horse ,Microbiology ,medicine ,Pulsed-field gel electrophoresis ,Animals ,Humans ,Horses ,General Veterinary ,biology ,Staphylococcal Infections ,biology.organism_classification ,Latex fixation test ,Italy ,Staphylococcus aureus ,pulsed-field gel electrophoresis ,Carrier State ,Female ,Horse Diseases ,Methicillin Resistance ,Coagulase ,medicine.drug - Abstract
Methicillin-resistant staphylococci (MRS) were isolated from nasal swabs of 56 of 159 (35.2%; 95% confidence interval [CI]: 27.9–43.2%) healthy horses. Two nasal swabs were collected from each horse; 43 of 159 (27%; 95% CI: 20.5–34.8%) of the cohort were colonized by MRS strains in 1 nostril, while in the remaining 13 of 159 (8.2%; 95% CI: 4.6–13.9%), different or identical MRS strains were isolated in both nostrils. Of the 29 humans in close contact with the horses tested, 4 (13.8%; 95% CI: 4.5–32.6%) were found to be carriers of MRS. All isolates were coagulase negative with the exception of 2 coagulase-positive MRS strains, Staphylococcus aureus and Staphylococcus pseudintermedius, both isolated from horses. To assay the methicillin resistance, a susceptibility test to oxacillin with standardized disk diffusion method, a PBP-2a latex agglutination test, and a methicillin resistance gene ( mecA) polymerase chain reaction assay were performed. Pulsed-field gel electrophoresis patterns of isolates from horses and humans in close contact with the horses revealed similarity. The results suggest evidence of transmission between animals, from animals to humans, and vice versa.
- Published
- 2010
196. Hepatocyte Growth Factor (HGF) Modulates Leydig Cells Extracellular Matrix Components
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M. Galdieri, Giulia Ricci, M. A. Tufano, Rita Canipari, Angela Catizone, B. Perfetto, Catizone, A., Ricci, Giulia, Tufano, M. A., Perfetto, Brunella, Canipari, R., and Galdieri, M.
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Male ,medicine.medical_specialty ,Urology ,Endocrinology, Diabetes and Metabolism ,Matrix metalloproteinase ,Extracellular matrix ,Endocrinology ,Transforming Growth Factor beta ,fibronectin ,Internal medicine ,Matrix Component ,medicine ,Animals ,uPA ,Secretion ,HGF ,TGF-beta ,Rats, Wistar ,Cells, Cultured ,biology ,Leydig cell ,Hepatocyte Growth Factor ,Leydig Cells ,Transforming growth factor beta ,Urokinase-Type Plasminogen Activator ,Extracellular Matrix ,Fibronectins ,Rats ,Cell biology ,Blot ,Fibronectin ,medicine.anatomical_structure ,Reproductive Medicine ,biology.protein ,Matrix Metalloproteinase 2 ,Hepatocyte growth factor ,medicine.drug - Abstract
Hepatocyte growth factor (HGF) is a pleiotropic factor that plays multiple roles during mammalian development. We previously demonstrated that in the postnatal testes, the HGF receptor, c-met, is expressed by Leydig cells and HGF increases the steroidogenetic activity of the cells. In the present article, we report that HGF modifies the composition of the extracellular matrix of cultured Leydig cells. We show that HGF increases the metabolic activity of isolated Leydig cells; in particular, the factor increases urokinase plasminogen activator and matrix metalloproteinase 2 secretion. We have also shown that the levels of active transforming growth factor beta are increased by HGF. On the contrary, using the Western blotting technique, a strong reduction in the amount of fibronectin present in the culture medium of cells cultured in the presence of HGF has been detected. The presented data demonstrate that HGF modulates several functional activities of Leydig cells, further supporting the hypothesis that this factor has a relevant role in the regulation of mammalian spermatogenesis.
- Published
- 2010
197. Osteopontin: a new emerging role in psoriasis
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Fabio Ayala, Maria Donnarumma, Nicola Balato, Elisabetta Buommino, Anna Balato, Maria Antonietta Tufano, Lucia Gallo, Nunzia Canozo, Buommino, Elisabetta, Tufano, M. A., Balato, N., Canozo, N., Donnarumma, M., Gallo, L., Balato, A., Ayala, F., Tufano, MARIA ANTONIETTA, Balato, Nicola, N., Canozo, Donnarumma, Maria, Gallo, Lucia, Balato, Anna, and Ayala, Fabio
- Subjects
Biopsy ,Integrin ,Interleukin-1beta ,Inflammation ,Dermatology ,Peripheral blood mononuclear cell ,Interferon-gamma ,Immune system ,Th2 Cells ,stomatognathic system ,Psoriasis ,Medicine ,Humans ,Osteopontin ,Skin ,biology ,business.industry ,CD44 ,Interleukin-8 ,Osteopontin Psoriasis Inflammation ,General Medicine ,Atopic dermatitis ,Th1 Cells ,medicine.disease ,Intercellular Adhesion Molecule-1 ,Hyaluronan Receptors ,Gene Expression Regulation ,Psoriasis · ,Immunology ,biology.protein ,Leukocytes, Mononuclear ,medicine.symptom ,business - Abstract
Osteopontin (OPN) is a phosphorylated acidic glycoprotein produced by cells of the immune system, epithelial tissue, smooth muscle cells, osteoblasts, and tumor cells. OPN interacts with integrins and CD44 to enhance Th1 and inhibit Th2 cytokine expression. The involvement of this molecule in the onset of psoriasis has not previously been studied. Here, we demonstrate that OPN is expressed in peripheral blood mononuclear cells and in skin biopsies of psoriatic patients. The study was conducted on 30 patients affected with plaque psoriasis, and on 11 healthy donors. Two blood samples and two skin samples from patients affected with atopic dermatitis were used as control for Th2 typical inflammatory skin disease. The analysis of IL-1beta, IFN-gamma, TauNuF-alpha, IL-8, and ICAM-1 showed the characteristic Th1 pattern in all the psoriatic blood and skin samples analyzed. This study offers an opportunity for understanding inflammation in psoriasis and supports the hypothesis that OPN could represent a potential target for therapeutic intervention in psoriatic patients.
- Published
- 2009
198. 5-AMINOLAUVULINIC ACID AND PHOTODYNAMIC THERAPY REDUCE HSV-1 REPLICATION IN HACAT CELLS THROUGH AN APOPTOSIS-INDIPENDENT MECHANISM
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Ayala F, Grimaldi E, Perfetto B, Donnarumma M, De Filippis A, Donnarumma G, Tufano MA, Ayala, F, Grimaldi, E, Perfetto, B, Donnarumma, M, De Filippis, A, Donnarumma, G, Tufano, M A, and Tufano, Ma
- Published
- 2009
199. Antimicrobial human beta-defensin-2 stimulates migration,proliferation and tube formation of humanumbilical vein endothelial cells
- Author
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Katia Bifulco, Maria Antonietta Tufano, Iole Paoletti, Maria Vincenza Carriero, Adone Baroni, Immacolata Longanesi-Cattani, Giovanna Donnarumma, Baroni, Adone, Donnarumma, Giovanna, Paoletti, I., LONGANESI CATTANI, I., Bifulco, K., Tufano, M. A., and Carriero, M. V.
- Subjects
Umbilical Veins ,beta-Defensins ,Endothelium ,Physiology ,Angiogenesis ,medicine.medical_treatment ,Biology ,Biochemistry ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Endocrinology ,Anti-Infective Agents ,Endothelial cell ,Cell Movement ,medicine ,Humans ,Cells, Cultured ,Cell Proliferation ,Tube formation ,Matrigel ,Growth factor ,Endothelial Cells ,Chemotaxi ,Beta-defensin ,Cell biology ,Endothelial stem cell ,Vascular endothelial growth factor ,medicine.anatomical_structure ,chemistry ,Immunology ,Endothelium, Vascular ,Wound healing - Abstract
Human beta-defensin-2 (hBD-2) is an antimicrobial peptide which is released upon microbial invasion and contributes to mucosal and epithelial defense modulating both innate and adaptive immunity. We found that hBD-2 stimulates chemotaxis of human endothelial cells with an extent similar to that exerted by the vascular endothelial growth factor (VEGF). The hBD-2-dependent chemotaxis is dose-dependent, maximal effect being reached at 500 ng/ml concentration. In the absence of any growth factor, hBD-2 favors wound healing of endothelial cells, causing an about 2-fold increase in the speed of wound closure with respect to the control. Furthermore, hBD-2 promotes endothelial cell proliferation, although at a minor extent as compared to VEGF. When plated on matrigel enriched with angiogenic factors, endothelial cells form a three-dimensional network of tubes that gives rise to capillary-like structures. Similarly to VEGF, hBD-2 promotes capillary-like tube formation of human endothelial cells. Pro-angiogenic effect promoted by hBD-2 is dose-dependent, peaks at a 500 ng/ml hBD-2 concentration and is prevented by blocking anti-alphavbeta3 monoclonal antibody. However, hBD-2-induced pro-angiogenic activity is not due to endogenously produced VEGF because it is not prevented by neutralizing anti-VEGF antibodies. Overall, our findings suggest that hBD-2 could link inflammation and the host defense through its pro-angiogenic activity.
- Published
- 2009
200. Failure of first line eradication treatment significantly increases prevalence of anti-microbial resistant helicobacter pylori clinical isolates
- Author
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Dolores Vaira, Gerardo Nardone, Alba Rocco, Maurizio Romano, A.P. Pilloni, D Cozzolino, Maria Rosaria Iovene, Maria Antonietta Tufano, R. Salerno, M I Russo, Romano M, Novene MR, Russo MI, Rocco A, Salerno R, Cozzolino D, Pilloni AP, Tufano MA, Vaira D, Nardone G., Romano, Marco, Iovene, Maria Rosaria, Russo, Mi, Rocco, A, Salerno, R, Cozzolino, D, Pilloni, Ap, Tufano, Ma, Vaira, D, Nardone, G., Romano, M., Iovene, M. R., Russo, M. I., Rocco, Alba, Salerno, R., Cozzolino, D., Pilloni, A. P., Tufano, M. A., Vaira, D., and Nardone, GERARDO ANTONIO PIO
- Subjects
Adult ,Male ,medicine.medical_specialty ,Ofloxacin ,Colony Count, Microbial ,Drug resistance ,Levofloxacin ,Gastroenterology ,Pathology and Forensic Medicine ,Microbiology ,Helicobacter Infections ,Antibiotic resistance ,Internal medicine ,Clarithromycin ,Metronidazole ,Drug Resistance, Bacterial ,medicine ,Humans ,Treatment Failure ,triple eradication therapy ,Aged ,GASTRITIS ,Chi-Square Distribution ,ANTIMICROBIAL RESISTANCE ,biology ,business.industry ,Tetracycline Resistance ,Amoxicillin ,HELICOBACTER PYLORI ,General Medicine ,TREATMENT ,Helicobacter pylori ,Middle Aged ,Antimicrobial ,biology.organism_classification ,Anti-Bacterial Agents ,"Helicobacter pylori" ,Case-Control Studies ,Female ,CLINICAL ISOLATES ,business ,medicine.drug - Abstract
Objectives:Helicobacter pylori infection is a major health problem worldwide, and effective eradication of the infection is mandatory. The efficacy of recommended eradication regimens is approximately 70%. To avoid treatment failure and the consequent development of secondary resistance(s), it is important to choose the most appropriate first-line treatment regimen. This choice should also be made based on the knowledge of the antimicrobial resistance peculiar to a given geographical area. We evaluated the prevalence of antimicrobial-resistant H pylori strains isolated from naive patients and from patients with previous unsuccessful treatments.Methods:This study examined 109 H pylori-infected subjects (Group 1) who had never received an eradication treatment and 104 H pylori-infected subjects (Group 2) who had failed one or more eradication treatments. Resistance to amoxicillin (AMO), tetracycline (TET), clarithromycin (CLA), metronidazole (MET) and levofloxacin (LEV) was determined using the epsilometer test. The significance of differences was evaluated by the χ2 test.Results:The prevalence of antimicrobial resistance was 0% versus 3.1% to AMO, 0% versus 2% to TET, 27% versus 41.3% to MET (pH pylori strains resistant to multiple antimicrobials.Conclusions:This study confirms the high prevalence of H pylori strains resistant to CLA and MET, and indicates that unsuccessful treatments significantly increase resistance. Choosing eradication regimens other than standard triple therapy as a first-line therapy should be advisable in areas with high primary antimicrobial resistance prevalence.
- Published
- 2008
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