Search

Your search keyword '"Tuberosa, Roberto"' showing total 958 results
Start Over Author "Tuberosa, Roberto"
958 results on '"Tuberosa, Roberto"'

151. Genome-wide association mapping for grain shape and color traits in Ethiopian durum wheat (Triticum turgidumssp. durum)

Author

Alemu, Admas, Feyissa, Tileye, Tuberosa, Roberto, Maccaferri, Marco, Sciara, Giuseppe, Letta, Tesfaye, and Abeyo, Bekele

Abstract

Grain shape and color strongly influence yield and quality of durum wheat. Identifying QTL for these traits is essential for transferring favorable alleles based on selection strategies and breeding objectives. In the present study, 192 Ethiopian durum wheat accessions comprising 167 landraces and 25 cultivars were genotyped with a high-density Illumina iSelect 90K single-nucleotide polymorphism (SNP) wheat array to conduct a genome-wide association analysis for grain width (GW), grain length (GL), CIE (Commission Internationale l'Eclairage) L* (brightness), CIE a* (redness), and CIE b* (yellowness) traits. The accessions were planted at Sinana Agricultural Research Center, Ethiopia in the 2015/2016 cropping season in a complete randomized block design with three replications. Twenty homogeneous and healthy seeds per replicate were used for trait measurement. Digital image analysis of seeds with GrainScan software package was used to generate the phenotypic data. Analysis of variance revealed highly significant differences between accessions for all traits. A total of 46 quantitative trait loci (QTL) were identified for all traits across all chromosomes. One novel major candidate QTL (−lg P ≥ 4) with pleiotropic effects for grain CIE L* (brightness) and CIE a* (redness) was identified on the long arm of chromosome 2A. Eighteen nominal QTL (−lg P ≥ 3) and 26 suggestive QTL (−lg P ≥ 2.5) were identified. Pleiotropic QTL influencing both grain shape and color were identified.

Published
2020
Full Text
View/download PDF

152. QTL meta-analysis for phenology and yield in maize: a possible link?

Author

SORIANO SORIANO, JOSE MIGUEL, MARTINEZ ASCANIO, ANA KARINE, TUBEROSA, ROBERTO, SALVI, SILVIO, KOUMPROGLOU R., JAHRMANN T., SORIANO SORIANO, J.M., MARTINEZ ASCANIO, A.K., KOUMPROGLOU R., JAHRMANN T., TUBEROSA R., and SALVI S.

Subjects
maize, flowering time, meta-analysis, yield
Published
2014

153. Mutagenesis, genomics and cytogenetics in Arundo donax

Author

VALLI, FABIO, TUBEROSA, ROBERTO, SALVI, SILVIO, Daniele Trebbi, Dagmara Gasior, John Harper, Ian Armstead3, Mila D'Angelantonio, Stefano Predieri, Fabio Valli, Daniele Trebbi, Dagmara Gasior, John Harper, Ian Armstead3, Mila D'Angelantonio, Stefano Predieri, Roberto Tuberosa, and Silvio Salvi

Subjects
Cytogenetics, Genotyping by Sequencing, Physical mutagenesi, Arundo donax
Published
2014

154. Durum wheat genomics comes of age

Author

TUBEROSA, ROBERTO, C. Pozniak, R. Tuberosa, and C. Pozniak

Subjects
ASSOCIATION MAPPING, QTL, Triticum turgidum L, DROUGHT, Root architecture
Abstract

no abstract

Published
2014

155. Leveraging genomics approaches to enhance abiotic stress resistance in cereals

Author

SALVI, SILVIO, MACCAFERRI, MARCO, FRASCAROLI, ELISABETTA, LANDI, PIERANGELO, CANE', MARIA ANGELA, SORIANO SORIANO, JOSE MIGUEL, GRAZIANI, MARTA, MILNER, SARA GIULIA, MARTINEZ ASCANIO, ANA KARINE, TUBEROSA, ROBERTO, Ulrike Lohwasser & Andreas Börner (eds.), Salvi, S, Maccaferri, M, Frascaroli, E, Landi, P, Cané, M A, Soriano Soriano, J M, Graziani, M, Milner, S, Martinez Ascanio, A K, and Tuberosa, R

Subjects
Drought, QTL, genomics, marker-assisted selection
Published
2014

160. Fine Mapping of qroot-yield-1.06, a QTL for Root, Plant Vigor and Yield in Maize

Author

Tuberosa, Roberto, Martinez Ascanio, Ana Karine <1979>, Tuberosa, Roberto, and Martinez Ascanio, Ana Karine <1979>

Abstract

Root-yield-1.06 is a major QTL affecting root system architecture (RSA) and other agronomic traits in maize. The effect of this QTL has been evaluated with the development of near isogenic lines (NILs) differing at the QTL position. The objective of this study was to fine map qroot-yield-1.06 by marker-assisted searching for chromosome recombinants in the QTL interval and concurrent root phenotyping in both controlled and field conditions, through successive generations. Complementary approaches such as QTL meta-analysis and RNA-seq were deployed in order to help prioritizing candidate genes within the QTL target region. Using a selected group of genotypes, field based root analysis by ‘shovelomics’ enabled to accurately collect RSA information of adult maize plants. Shovelomics combined with software-assisted root imaging analysis proved to be an informative and relatively highly automated phenotyping protocol. A QTL interval mapping was conducted using a segregating population at the seedling stage grown in controlled environment. Results enabled to narrow down the QTL interval and to identify new polymorphic markers for MAS in field experiments. A collection of homozygous recombinant NILs was developed by screening segregating populations with markers flanking qroot-yield-1.06. A first set of lines from this collection was phenotyped based on the adapted shovelomics protocol. QTL analysis based on these data highlighted an interval of 1.3 Mb as completely linked with the target QTL but, a larger safer interval of 4.1 Mb was selected for further investigations. QTL meta-analysis allows to synthetize information on root QTLs and two mQTLs were identified in the qroot-yield-1.06 interval. Trascriptomics analysis based on RNA-seq data of the two contrasting QTL-NILs, confirmed alternative haplotypes at chromosome bin 1.06. qroot-yield-1.06 has now been delimited to a 4.1-Mb interval, and thanks to the availability of additional untested homozygous recombinant NILs, t

Published
2015

164. Genetic dissection of fusarium head blight response in a durum wheat elite population

Author

MACCAFERRI, MARCO, CORNETI, SIMONA, RICCI, ANDREA, STEFANELLI, SANDRA, PRODI, ANTONIO, PISI, ANNAMARIA, TUBEROSA, ROBERTO, Braida E., Lancioni P., Ammar K., Pascale M., Lippolis V., Massi A., Maccaferri M., Corneti S., Ricci A., Stefanelli S., Braida E., Lancioni P., Ammar K., Prodi A., Pisi A., Pascale M., Lippolis V., Massi A., and Tuberosa R.

Subjects
FUSARIUM HEAD BLIGHT, DURUM WHEAT
Published
2013

165. Physical mutagenesis of the bioenergy species Arundo donax L

Author

Daniele Trebbi, Mila D'Angelantonio, Stefano Predieri, VALLI, FABIO, TUBEROSA, ROBERTO, SALVI, SILVIO, Daniele Trebbi, Fabio Valli, Mila D'Angelantonio, Stefano Predieri, Roberto Tuberosa, and Silvio Salvi

Subjects
Arundo donax L, Physical mutagenesi
Abstract

Arundo donax L. (giant reed) is a perennial C3 Poaceae species of emerging interest for bioenergy and phytoremediation exploitation (Fig. 1). Recent investigations highlighted its high efficiency as to carbon accumulation and biomass production (>40 t of dry matter ha-1 year-1; Angelini et al. 2008), and for second generation bioethanol production. A. donax completely lacks sexual reproduction and only propagates vegetatively. As a consequence, very little genetic variation is found between naturally occurring ecotypes, precluding any possibility to implement breeding program based on selection (Mariani et al. 2010, Hardion et al. 2012).

Published
2013

167. A multiparental cross population for mapping yield QTLs in durum wheat

Author

MILNER, SARA GIULIA, MACCAFERRI, MARCO, CORNETI, SIMONA, STEFANELLI, SANDRA, SALVI, SILVIO, TUBEROSA, ROBERTO, Mantovani P., Massi A., Milner S.G., Maccaferri M., Corneti S., Stefanelli S., Mantovani P., Massi A., Salvi S., and Tuberosa R.

Subjects
QTL
Published
2013

168. Mapping QTLs for Fusarium head blight response in a durum wheat elite population

Author

MACCAFERRI, MARCO, CORNETI, SIMONA, RICCI, ANDREA, STEFANELLI, SANDRA, LANCIONI, PIETRO, PRODI, ANTONIO, PISI, ANNAMARIA, TUBEROSA, ROBERTO, Braida E, Ammar K, Pascale M, Lippolis V, Massi A, INRA, Maccaferri M, Corneti S, Ricci A, Stefanelli S, Braida E, Lancioni P, Ammar K, Prodi A, Pisi A, Pascale M, Lippolis V, Massi A, and Tuberosa R

Subjects
fusarium head light, mycotoxin
Published
2013

169. Mapping QTLs for Fusarium head blight response in a durum elite population

Author

MACCAFERRI, MARCO, CORNETI, SIMONA, RICCI, ANDREA, STEFANELLI, SANDRA, LANCIONI, PIETRO, PRODI, ANTONIO, PISI, ANNAMARIA, TUBEROSA, ROBERTO, Braida E., Ammar K., Pascale M., Lippolis V., Massi A., Maccaferri M., Corneti S., Ricci A., Stefanelli S., Braida E., Lancioni P., Ammar K., Prodi A., Pisi A., Pascale M., Lippolis V., Massi A., and Tuberosa R.

Subjects
FUSARIUM HEAD BLIGHT, DURUM WHEAT
Published
2013

172. Transferability of bread wheat SNPs in durum wheat

Author

SALVI, SILVIO, TUBEROSA, ROBERTO, MACCAFERRI, MARCO, Ricci A., Vendramin V., Morgante M., Sanguineti M. C., Massi A., Salvi S., Maccaferri M., Ricci A., Vendramin V., Morgante M., Sanguineti M.C., Massi A., and Tuberosa R.

Subjects
SNP, TRITICUM AESTIVUM, MARKER ASSISTED SELECTION, TRITICUM DURUM
Published
2012

173. Discovery of chemically induced mutations by TILLING

Author

Bovina R., Talamé V., Sanguineti M. C., SALVI, SILVIO, TUBEROSA, ROBERTO, Shu Q.Y., Forster B.P., Nakagawa H., Bovina R., Talamé V., Salvi S., Sanguineti M.C., and Tuberosa R.

Subjects
MUTAGENESIS, TILLING, BARLEY, FUNCTIONAL GENOMICS
Abstract

The term functional genomics encompasses a number of different approaches aimed at determining gene function on a genome-wide scale. The application of these approaches is greatly facilitated by the utilisation of new, high-throughput technologies applicable to almost any organism. As an example, sequence alignment-based comparisons are used to identify homologous sequences between and within species, transcriptional profiling to determine gene expression patterns and interaction analyses to help elucidate pathways, networks and protein complexes. However, although these analyses are extremely useful to extrapolate important features of a novel gene from a biochemical or a molecular point of view, they are not very informative in the context of the functional complexity of a living organism. In order to overcome these limitations, different reverse-genetics approaches have been conceived. Nonetheless, the tools for reverse genetics are not always transferable from one organism to another or from model species to non-model ones because in most cases the main drawback is the lack of efficient technical protocols exploitable for the majority of the plant species. A novel, reverse-genetics approach that combines the advantages of point mutations provided by chemical mutagenesis, with the advantages of PCR-based mutational screening has been introduced recently under the name of TILLING (Targeting Induced Local Lesion IN Genomes; McCallum et al. 2000). From a technical standpoint, the first step of a TILLING assay is the PCR amplification of a target DNA fragment of interest from pooled DNAs of multiple mutant individuals. In sample pools, heteroduplexes with a mismatched base pair are formed between wild-type and mutated fragments by denaturing and reannealing PCR products (Fig 21.1). Heteroduplexes are cleaved by an endonuclease enzyme able to recognize the mismatch position. Cleaved products are then resolved using denaturing polyacrilamide gel or capillary electrophoresis. When a positive signal is identified, individual DNA samples of the pools are mixed in equal amounts with the wild-type DNA and one-by-one reanalysed to identify the mutant individual plant; the induced mutations are eventually confirmed by sequencing. A detailed description of the technical aspects of the TILLING procedure is presented in the following section.

Published
2012

174. Omics Techniques in Crop Research: An OverviewImproving Crop Resistance to Abiotic Stress

Author

Bashasab Fakrudin, Rajeev K. Varshney, TUBEROSA, ROBERTO, N. Tuteja, S. S. Gill, A. F. Tiburcio and R. Tuteja, Bashasab Fakrudin, Roberto Tuberosa, and Rajeev K. Varshney

Subjects
high-throughput genotyping, phenomics, ESTs, SAGE, MPSS, next-generation sequencing
Abstract

Omics is a collective, broad discipline largely referring to analysis of the interactions of biological information obtained from the profiling of the genome, transcriptome, proteome, metabolome, and several other relevant -omes. While phase one of omics technologies aims at nontargeted identification of transcripts, proteins, and metabolites (essentially gene products) in a given biological sample, phase two deals with a very challenging analysis of data eventually leading to the dissection of the qualitative and quantitative dynamics of biological systems. Essentially, the omics science is enabled by a host of diverse, high-throughput technologies and platforms [1]. The full range of omics technologies can now be applied to understand the same fundamental biological processes [2]. Mapping and defining the relationships among genes, proteins, and metabolites require relative comparison of the networks that eventually help in understanding the regulatory mechanisms. A diverse but converging approaches such as forward and reverse genetics and transgenics (overexpression and knockdowns) can define the function of a gene to the specific phenotype, the omic technologies aim at revealing the function of each and every gene in the genome, which collectively contribute toward elucidating the networks and better understanding the whole plant phenotype [3–5].Access to omics tools at an affordable price is becoming a reality, which together with a large inventory of candidate genes, proteins, and metabolites and their databases deduced from profiling efforts in model systems and crop plants have speeded up the analysis of biological functions operating in various plant stress responses [2, 6]. These new strategies have begun to piece together the physiological and phenotypic observations with information on transcription and transcript regulation, the behavior of proteins, protein complexes and pathways, and the metabolites and metabolite fluxes, finally shedding light on evolutionary adaptive diversifications of organisms.

Published
2012

178. Molecular breeding for a changing climate: bridging ecophysiology and molecular biology

Author

TUBEROSA, ROBERTO, MACCAFERRI, MARCO, COLALONGO, MARIA CHIARA, SALVI, SILVIO, ARAUS J. L., SLAFER G. A., TUBEROSA R., MACCAFERRI M., COLALONGO M.C., and SALVI S.

Subjects
fungi, food and beverages, MOLECULAR MARKERS, MARKER ASSISTED SELECTION, GENOMICS, YIELD POTENTIAL
Abstract

Th is chapter surveys how genomics approaches have helped to elucidate the genetic basis of crop performance under abiotic constraints, such as drought, salinity, soil toxicity and low nutrients. Additionally, the review shows how this information has been used in breeding programmes to improve crop performance and how it might help agriculture better to withstand the negative effects of climate change. Future perspectives are given.

Published
2011

179. Leaf rust and powdery mildew resistance factors in durum wheat: identification and development of breeding-exploitable molecular markers

Author

MACCAFERRI, MARCO, BASSI, FILIPPO, TERRACCIANO, IRMA, MANTOVANI, PAOLA, BINI, FEDERICA, MASSI A., KOLMER J., AMMAR K., CZEMBOR J., KELLER B., SIMKOVA H., TUBEROSA, ROBERTO, SANGUINETI, MARIA CORINNA, MACCAFERRI M., BASSI F., TERRACCIANO I., MANTOVANI P., BINI F., MASSI A., KOLMER J., AMMAR K., CZEMBOR J., KELLER B., SIMKOVA H., TUBEROSA R., and SANGUINETI M.C.

Subjects
wheat, powdery mildew, QTL, genetic mapping
Published
2011

180. Genetic control of resistance to s-borne cereal mosaic virus (SBCMV) in durum wheat. Atti XV Congresso SiPAV, Bologna, p.81

Author

MACCAFERRI, MARCO, RATTI, CLAUDIO, RUBIES AUTONELL, CONCEPCION, STEFANELLI, SANDRA, TUBEROSA, ROBERTO, Francia R., Massi A., Sanguineti MC, SOCIETÀ ITALIANA DI PATLOGIA VEGETALE, Maccaferri M., Francia R., Ratti C., Rubies Autonell C., Massi A., Stefanelli S., Tuberosa R., and Sanguineti MC

Subjects
viru, durum wheat, food and beverages, SOIL-BORNE, soil-borne cereal mosaic virus, wheat, genetic mapping, marker assisted selection
Abstract

Soil-borne cereal mosaic (SBCM) is a viral disease which affects wheat in Europe. In durum wheat, the cultivated germplasm shows responses ranging from susceptibility to almost complete resistance. Genetic analysis of SBCMV resistance was carried out using two recombinant inbred line (RIL) populations of 180 lines each, obtained from the crosses Meridiano (resistant) × Claudio (moderately susceptible) and Simeto (susceptible) × Levante (resistant). The RILs were characterized for SBCMV response (symptom severity scores and virus concentration in leaves) in the field in 2007, 2008 and 2009 under severe and uniform SBCMV infections and were genotyped with SSR and DArT® markers. Transgressive segregation was observed for the disease response traits, with high heritability values, consistently > 80%. A major QTL (QSbm.ubo-2BS) in the distal chromosome 2BS region was identified in both populations; it accounted for 60-85% of the phenotypic variation for symptom severity, 40-55% for virus concentration and 15-30% for grain yield. The meta-QTL analysis carried out using the entire dataset suggested that in both populations SBCMV resistance is likely to be controlled by the same QTL, with the favorable allele being contributed by Meridiano and Levante. Our results confine QSbm.ubo-2BS in a ca. 2 cM-wide interval flanked by SSR markers that are being used for marker-assisted selection. Several QTLs with minor effects were also detected. The presence of QSbm.ubo-2BS was validated in a panel of 111 cultivated durum wheat accessions. The reported results open the possibility to further characterize QSbm.ubo-2BS and suggest its feasibility for a positional cloning approach.

Published
2011

182. Global agricultural intensification during climate change: a role for genomics

Author

Abberton, Michael, primary, Batley, Jacqueline, additional, Bentley, Alison, additional, Bryant, John, additional, Cai, Hongwei, additional, Cockram, James, additional, Costa de Oliveira, Antonio, additional, Cseke, Leland J., additional, Dempewolf, Hannes, additional, De Pace, Ciro, additional, Edwards, David, additional, Gepts, Paul, additional, Greenland, Andy, additional, Hall, Anthony E., additional, Henry, Robert, additional, Hori, Kiyosumi, additional, Howe, Glenn Thomas, additional, Hughes, Stephen, additional, Humphreys, Mike, additional, Lightfoot, David, additional, Marshall, Athole, additional, Mayes, Sean, additional, Nguyen, Henry T., additional, Ogbonnaya, Francis C., additional, Ortiz, Rodomiro, additional, Paterson, Andrew H., additional, Tuberosa, Roberto, additional, Valliyodan, Babu, additional, Varshney, Rajeev K., additional, and Yano, Masahiro, additional

Published
2015
Full Text
View/download PDF

183. The Genetic Basis of Composite Spike Form in Barley and ‘Miracle-Wheat’

Author

Poursarebani, Naser, primary, Seidensticker, Tina, additional, Koppolu, Ravi, additional, Trautewig, Corinna, additional, Gawroński, Piotr, additional, Bini, Federica, additional, Govind, Geetha, additional, Rutten, Twan, additional, Sakuma, Shun, additional, Tagiri, Akemi, additional, Wolde, Gizaw M, additional, Youssef, Helmy M, additional, Battal, Abdulhamit, additional, Ciannamea, Stefano, additional, Fusca, Tiziana, additional, Nussbaumer, Thomas, additional, Pozzi, Carlo, additional, Börner, Andreas, additional, Lundqvist, Udda, additional, Komatsuda, Takao, additional, Salvi, Silvio, additional, Tuberosa, Roberto, additional, Uauy, Cristobal, additional, Sreenivasulu, Nese, additional, Rossini, Laura, additional, and Schnurbusch, Thorsten, additional

Published
2015
Full Text
View/download PDF

192. Searching for QTLs to enhance yield stability in durum wheat

Author

MACCAFERRI, MARCO, SANGUINETI, MARIA CORINNA, BASSI, FILIPPO, CORNETI, SIMONA, STEFANELLI, SANDRA, TUBEROSA, ROBERTO, GRAZIANI M., DEMONTIS A., MASSI A., AMMAR K., PAUX E., FEUILLET C., MACCAFERRI M., SANGUINETI M.C., GRAZIANI M., BASSI F., CORNETI S., STEFANELLI S., DEMONTIS A., MASSI A., AMMAR K., PAUX E., FEUILLET C., and TUBEROSA R.

Published
2010

195. Fine mapping of QYld.idw-3B, a major QTL on chromosome 3BS for yield in durum wheat

Author

GRAZIANI M., PAUX E., SALSE J., CHOULET F., FEUILLET C., DEMONTIS A., MACCAFERRI, MARCO, SANGUINETI, MARIA CORINNA, STEFANELLI, SANDRA, TERRACCIANO, IRMA, TUBEROSA, ROBERTO, GRAZIANI M., MACCAFERRI M., SANGUINETI M.C., PAUX E., SALSE J., CHOULET F., FEUILLET C., STEFANELLI S., DEMONTIS A., TERRACCIANO I., and TUBEROSA R.

Published
2010

196. QTL mapping for root characteristics at the seedling stage in maize

Author

GIULIANI, SILVIA, BUSATTO, CARLA, SALVI, SILVIO, RICCIOLINI, CLAUDIA, CARRARO, NICOLA, SANGUINETI, MARIA CORINNA, TUBEROSA, ROBERTO, PRESTERL T., OUZUNOVA M., GIULIANI S., BUSATTO C., SALVI S., RICCIOLINI C., CARRARO N., PRESTERL T., OUZUNOVA M., SANGUINETI M.C., and TUBEROSA R.

Published
2010

197. QTL mapping and marker-assisted selection in durum wheat

Author

MACCAFERRI, MARCO, SANGUINETI, MARIA CORINNA, MANTOVANI, PAOLA, BASSI, FILIPPO, CORNETI, SIMONA, CHEN, FENG, BINI, FEDERICA, TUBEROSA, ROBERTO, DEMONTIS A., MASSI A., MACCAFERRI M., SANGUINETI M.C., MANTOVANI P., BASSI F., CORNETI S., CHEN F., BINI F., DEMONTIS A., MASSI A., and TUBEROSA R.

Published
2010

198. Genetics of Soil-borne cereal mosaic virus (Sbcmv) response in durum wheat

Author

MACCAFERRI, MARCO, SANGUINETI, MARIA CORINNA, RATTI, CLAUDIO, RUBIES AUTONELL, CONCEPCION, BINI, FEDERICA, BADIALI, FEDERICA, STEFANELLI, SANDRA, TUBEROSA, ROBERTO, VALLEGA V., DEMONTIS A., FRISON E., GRANER A., TUBEROSA R., MACCAFERRI M., SANGUINETI M.C., RATTI C., RUBIES AUTONELL C., VALLEGA V., BINI F., BADIALI F., STEFANELLI S., DEMONTIS A., and TUBEROSA R.

Published
2010

Catalog

Books, media, physical & digital resources
See catalog results