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153. Phenotyping the Chilling and Freezing Responses of Young Microspore Stage Wheat Spikes Using Targeted Metabolome and Lipidome Profiling

Author

Thomas Ben Biddulph, Rudy Dolferus, Olive Onyemaobi, Ute Roessner, William Ho, Bo Eng Cheong, and Thusitha Rupasinghe

Subjects
0106 biological sciences, 0301 basic medicine, phenotyping, Biology, 01 natural sciences, Article, 03 medical and health sciences, Metabolomics, Microspore, wheat, Freezing, Cold acclimation, Metabolome, Amines, Amino Acids, lcsh:QH301-705.5, Triticum, Abiotic stress, Primary metabolite, food and beverages, General Medicine, spike, cold tolerance, Lipidome, Lipid Metabolism, metabolomics, reproductive development, Plant Leaves, Horticulture, Phenotype, 030104 developmental biology, lcsh:Biology (General), Pollen, lipidomics, Frost (temperature), 010606 plant biology & botany
Abstract

Chilling and frost conditions impose major yield restraints to wheat crops in Australia and other temperate climate regions. Unpredictability and variability of field frost events are major impediments for cold tolerance breeding. Metabolome and lipidome profiling were used to compare the cold response in spikes of cold-tolerant Young and sensitive variety Wyalkatchem at the young microspore (YM) stage of pollen development. We aimed to identify metabolite markers that can reliably distinguish cold-tolerant and sensitive wheat varieties for future cold-tolerance phenotyping applications. We scored changes in spike metabolites and lipids for both varieties during cold acclimation after initial and prolonged exposure to combined chilling and freezing cycles (1 and 4 days, respectively) using controlled environment conditions. The two contrasting wheat varieties showed qualitative and quantitative differences in primary metabolites involved in osmoprotection, but differences in lipid accumulation most distinctively separated the cold response of the two wheat lines. These results resemble what we previously observed in flag leaves of the same two wheat varieties. The fact that this response occurs in tissue types with very different functions indicates that chilling and freezing tolerance in these wheat lines is associated with re-modelling of membrane lipid composition to maintain membrane fluidity.

Published
2020
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154. An optimized metaproteomics protocol for a holistic taxonomic and functional characterization of microbial communities from marine particles

Author

Stephanie Markert, Jörg Bernhardt, Daniela Zühlke, Dirk Albrecht, Claudia Hirschfeld, Doreen Schultz, Katharina Riedel, and Thomas Ben Francis

Subjects
Cyanobacteria, Proteomics, 03 medical and health sciences, Bacterial Proteins, Tandem Mass Spectrometry, Protein purification, Lysis buffer, Seawater, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 0303 health sciences, biology, Bacteria, 030306 microbiology, Microbiota, Eutrophication, biology.organism_classification, Agricultural and Biological Sciences (miscellaneous), Biochemistry, Metagenomics, Metaproteomics, Metagenome, North Sea, Proteobacteria, Flavobacteriia, Chromatography, Liquid
Abstract

This study aimed to establish a robust and reliable metaproteomics protocol for an in-depth characterization of marine particle-associated (PA) bacteria. To this end, we compared six well-established protein extraction protocols together with different MS-sample preparation techniques using particles sampled during a North Sea spring algae bloom in 2009. In the final optimized workflow, proteins are extracted using a combination of SDS-containing lysis buffer and cell disruption by bead-beating, separated by SDS-PAGE, in-gel digested and analysed by LC-MS/MS, before MASCOT search against a metagenome-based database and data processing/visualization with the in-house-developed bioinformatics tools Prophane and Paver. As an application example, free-living (FL) and particulate communities sampled in April 2009 were analysed, resulting in an as yet unprecedented number of 9354 and 5034 identified protein groups for FL and PA bacteria, respectively. Our data suggest that FL and PA communities appeared similar in their taxonomic distribution, with notable exceptions: eukaryotic proteins and proteins assigned to Flavobacteriia, Cyanobacteria, and some proteobacterial genera were found more abundant on particles, whilst overall proteins belonging to Proteobacteria were more dominant in the FL fraction. Furthermore, our data points to functional differences including proteins involved in polysaccharide degradation, sugar- and phosphorus uptake, adhesion, motility, and stress response.

Published
2020

156. Edgar Wind and Modern Art: In Defence of Marginal Anarchy

Author

Thomas, Ben and Thomas, Ben

Abstract

This book presents the first comprehensive study of the philosopher and art historian Edgar Wind's critique of modern art. The first student of Erwin Panofsky, and a close associate of Aby Warburg, Edgar Wind was unusual among the 'Warburgians' for his sustained interest in modern art, together with his support for contemporary artists. This culminated in his respected and influential book Art and Anarchy (1963), which seemed like a departure from his usual scholarly work on the iconography of Renaissance art. Based on extensive archival research and bringing to light previously unpublished lectures, Edgar Wind and Modern Art reveals the extent and seriousness of Wind's thinking about modern art, and how it was bound up with theories about art and knowledge that he had developed during the 1920s and 30s. Wind's ideas are placed in the context of a closely connected international cultural milieu consisting of some of the leading artists and thinkers of the twentieth century. In particular, the book discusses in detail his friendships with three significant artists: Pavel Tchelitchew, Ben Shahn and R. B. Kitaj. In the process, the existence of an alternative to the prevailing formalist approach of Alfred Barr and Clement Greenberg to modern art, based on the enduring importance of the symbol, is revealed.

Published
2020

157. Comparison between Thermophysical and Tribological Properties of Two Engine Lubricant Additives: Electrochemically Exfoliated Graphene and Molybdenum Disulfide Nanoplatelets

Author

Universidade de Santiago de Compostela. Departamento de Física Aplicada, García Guimarey, María Jesús, Abdelkader, Amor M., Comuñas, Maria JP, Álvarez Lorenzo, Carmen Isabel, Thomas, Ben, Fernández, Josefa, Hadfield, Mark, Universidade de Santiago de Compostela. Departamento de Física Aplicada, García Guimarey, María Jesús, Abdelkader, Amor M., Comuñas, Maria JP, Álvarez Lorenzo, Carmen Isabel, Thomas, Ben, Fernández, Josefa, and Hadfield, Mark

Abstract

Recently graphene and other 2D materials were suggested as nano additives to enhance the performance of nanolubricants and reducing friction and wear-related failures in moving mechanical parts. Nevertheless, to our knowledge there are no previous studies on electrochemical exfoliated nanomaterials as lubricant additives. In this work, engine oil-based nanolubricants were developed via two-steps method using two different 2D nanomaterials: a carbon-based nano additive, graphene nanoplatelets (GNP) and a sulphide nanomaterial, molybdenum disulfide (MoS2) nanoplatelets (MSNP). The influence of these nano additives on the thermophysical properties of the nanolubricants, such as viscosity index, density and wettability, was investigated. The unique features of the electrochemical exfoliated GNP and MSNP allow the formulation of nanolubricant with unusual thermophysical properties. Both the viscosity and density of the nanolubricants decreased by increasing the nanoplatelets loading. The effect of the nano additives loading and temperature on the tribological properties of nanolubricants was investigated using two different test configurations: reciprocating ball-on-plate and rotational ball-on-three-pins. The tribological specimens were analysed by scanning electron microscopy (SEM) and 3D profiler in order to evaluate the wear. The results showed significant improvement in the antifriction and anti-wear properties, for the 2D-materials-based nanolubricants as compared with the engine oil, using different contact conditions. For the reciprocal friction tests, maximum friction and worn area reductions of 20% and 22% were achieved for the concentrations of 0.10 wt% and 0.20 wt% GNP, respectively. Besides, the best anti-wear performance was found for the nanolubricant containing 0.05 wt% MSNP in rotational configuration test, with reductions of 42% and 60% in the scar width and depth, respectively, with respect to the engine oil.

Published
2020

158. Raphael: Drawing and eloquence

Author

Thomas, Ben, Whistler, Catherine, Thomas, Ben, and Whistler, Catherine

Abstract

The volume collects nine essays particularly dedicated to drawing, one of the most popular themes in the historical-artistic research dedicated to Raphael. The cut given to the whole goes under the great thematic category of rhetoric. The aspects concerning the relationship between image and word, grace, the construction of particular types of figures and the interaction between poetry and drawing in the sheets of the Sonnets are probed. [Il volume raccoglie nove saggi particolarmente dedicati al disegno, uno de temi più frequentati nella ricerca storico-artistica dedicata a Raffaello. Il taglio dato all’insieme va sotto la grande categoria tematica della retorica. Vengono sondati gli aspetti riguardanti il rapporto tra immagine e parola, la grazia, la costruzione di particolari tipologie di figure e l’interazione tra poesia e disegno nei fogli dei Sonetti.]

Published
2020

160. Derivation and validation of a clinical severity score for acutely ill adults with suspected COVID-19: The PRIEST observational cohort study

Author

Goodacre, Steve, primary, Thomas, Ben, additional, Sutton, Laura, additional, Burnsall, Matthew, additional, Lee, Ellen, additional, Bradburn, Mike, additional, Loban, Amanda, additional, Waterhouse, Simon, additional, Simmonds, Richard, additional, Biggs, Katie, additional, Marincowitz, Carl, additional, Schutter, Jose, additional, Connelly, Sarah, additional, Sheldon, Elena, additional, Hall, Jamie, additional, Young, Emma, additional, Bentley, Andrew, additional, Challen, Kirsty, additional, Fitzsimmons, Chris, additional, Harris, Tim, additional, Lecky, Fiona, additional, Lee, Andrew, additional, Maconochie, Ian, additional, and Walter, Darren, additional

Published
2021
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162. Post-exertion oxygen saturation as a prognostic factor for adverse outcome in patients attending the emergency department with suspected COVID-19: a substudy of the PRIEST observational cohort study

Author

Goodacre, Steve, primary, Thomas, Ben, additional, Lee, Ellen, additional, Sutton, Laura, additional, Loban, Amanda, additional, Waterhouse, Simon, additional, Simmonds, Richard, additional, Biggs, Katie, additional, Marincowitz, Carl, additional, Schutter, José, additional, Connelly, Sarah, additional, Sheldon, Elena, additional, Hall, Jamie, additional, Young, Emma, additional, Bentley, Andrew, additional, Challen, Kirsty, additional, Fitzsimmons, Chris, additional, Harris, Tim, additional, Lecky, Fiona, additional, Lee, Andrew, additional, Maconochie, Ian, additional, and Walter, Darren, additional

Published
2020
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163. Characterisation of 22445 patients attending UK emergency departments with suspected COVID-19 infection: Observational cohort study

Author

Goodacre, Steve, primary, Thomas, Ben, additional, Lee, Ellen, additional, Sutton, Laura, additional, Loban, Amanda, additional, Waterhouse, Simon, additional, Simmonds, Richard, additional, Biggs, Katie, additional, Marincowitz, Carl, additional, Schutter, Jose, additional, Connelly, Sarah, additional, Sheldon, Elena, additional, Hall, Jamie, additional, Young, Emma, additional, Bentley, Andrew, additional, Challen, Kirsty, additional, Fitzsimmons, Chris, additional, Harris, Tim, additional, Lecky, Fiona, additional, Lee, Andrew, additional, Maconochie, Ian, additional, and Walter, Darren, additional

Published
2020
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175. Derivation and validation of a clinical severity score for acutely ill adults with suspected COVID-19: The PRIEST observational cohort study

Author

Goodacre, Steve, primary, Thomas, Ben, additional, Sutton, Laura, additional, Bursnall, Matthew, additional, Lee, Ellen, additional, Bradburn, Mike, additional, Loban, Amanda, additional, Waterhouse, Simon, additional, Simmonds, Richard, additional, Biggs, Katie, additional, Marincowitz, Carl, additional, Schutter, Jose, additional, Connelly, Sarah, additional, Sheldon, Elena, additional, Hall, Jamie, additional, Young, Emma, additional, Bentley, Andrew, additional, Challen, Kirsty, additional, Fitzimmons, Chris, additional, Harris, Tim, additional, Lecky, Fiona, additional, Lee, Andrew, additional, Maconochie, Ian, additional, and Walter, Darren, additional

Published
2020
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177. Prognostic accuracy of emergency department triage tools for adults with suspected COVID-19: The PRIEST observational cohort study

Author

Thomas, Ben, primary, Goodacre, Steve, additional, Lee, Ellen, additional, Sutton, Laura, additional, Loban, Amanda, additional, Waterhouse, Simon, additional, Simmonds, Richard, additional, Biggs, Katie, additional, Marincowitz, Carl, additional, Schutter, Jose, additional, Connelly, Sarah, additional, Sheldon, Elena, additional, Hall, Jamie, additional, Young, Emma, additional, Bentley, Andrew, additional, Challen, Kirsty, additional, Fitzimmons, Chris, additional, Harris, Tim, additional, Lecky, Fiona, additional, Lee, Andrew, additional, Maconochie, Ian, additional, and Walter, Darren, additional

Published
2020
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186. Shockwave Lithotripsy Is an Efficacious Treatment Modality for Obese Patients with Upper Ureteral Calculi: Logistic Regression and Matched-Pair Analyses from a Dedicated Center Comparing Treatment Outcomes by Skin-to-Stone Distance

Author

Mains, Edward A.A., primary, Blackmur, James P., additional, Sharma, Abhishek D., additional, Gietzmann, William K., additional, El-Mokadem, Ismail, additional, Stephenson, Carolann, additional, Wallace, Shirley, additional, Phipps, Simon, additional, Thomas, Ben G., additional, Tolley, David A., additional, and Cutress, Mark L., additional

Published
2020
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195. Online retailers pitch partnerships

Author

Thomas, Ben

Subjects
Construction industry -- E-commerce, Construction industry -- Alliances and partnerships, Hot tubs -- Marketing, Retail industry -- E-commerce, Retail industry -- Alliances and partnerships, Swimming pools -- Marketing, Technicians -- Alliances and partnerships, Swimming pool industry -- E-commerce, Swimming pool industry -- Alliances and partnerships, Company marketing practices, Business, Construction and materials industries, Environmental services industry
Published
2010

196. Tracking down cracks

Author

Thomas, Ben

Subjects
Swimming pools -- Maintenance and repair, Business, Construction and materials industries, Environmental services industry
Published
2010

197. Tales of 'cut' cyanuric acid persist throughout the industry, and some techs think China is to blame

Author

Thomas, Ben

Subjects
Chemical industry -- Imports, Chemical industry -- Product defects and recalls, Herbicides -- Imports, Herbicides -- Product defects and recalls, Pesticides industry -- Imports, Pesticides industry -- Product defects and recalls, Construction industry -- Equipment and supplies, Business, Construction and materials industries, Environmental services industry
Published
2010

198. A tough call

Author

Thomas, Ben

Subjects
Construction industry -- Human resource management, Construction industry -- Downsizing, Cost (Economics) -- Management, Company personnel management, Company business management, Business, Construction and materials industries, Environmental services industry
Published
2010

200. Playing it safe

Author

Thomas, Ben

Subjects
Construction industry -- Surveys, Hot tubs -- Safety and security measures, Product safety -- Laws, regulations and rules, Product safety -- Surveys, Swimming pools -- Safety and security measures, Swimming pool industry -- Surveys, Safety education, Government regulation, Business, Construction and materials industries, Environmental services industry
Published
2010

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