151. A genome-wide screen identifies genes required for centromeric cohesion.
- Author
-
Marston AL, Tham WH, Shah H, and Amon A
- Subjects
- Cell Cycle, Cell Cycle Proteins genetics, Cell Cycle Proteins physiology, Chromosomal Proteins, Non-Histone, Chromosomes, Fungal physiology, Cytoskeletal Proteins genetics, Cytoskeletal Proteins physiology, Fungal Proteins, Gene Deletion, Genome, Fungal, Green Fluorescent Proteins, Luminescent Proteins, Mitosis, Mutation, Nondisjunction, Genetic, Nuclear Proteins genetics, Nuclear Proteins metabolism, Nuclear Proteins physiology, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Spores, Fungal physiology, Cohesins, Centromere physiology, Chromosome Segregation, Genes, Fungal, Meiosis, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae physiology, Saccharomyces cerevisiae Proteins physiology
- Abstract
During meiosis, two chromosome segregation phases follow a single round of DNA replication. We identified factors required to establish this specialized cell cycle by examining meiotic chromosome segregation in a collection of yeast strains lacking all nonessential genes. This analysis revealed Sgo1, Chl4, and Iml3 to be important for retaining centromeric cohesin until the onset of anaphase II. Consistent with this role, Sgo1 localizes to centromeric regions but dissociates at the onset of anaphase II. The screen described here provides a comprehensive analysis of the genes required for the meiotic cell cycle and identifies three factors important for the stepwise loss of sister chromatid cohesion.
- Published
- 2004
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