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Background Thaumatin II, a supersweet protein from the African plant katemfe (Thaumatococcus daniellii Benth.), shows promise as a zero-calorie sweetener for use in the food and pharmaceutical industries and for improving the taste of fruit. Results We report on the stability of thaumatin in salted and pickled tomatoes, as well as on the effect of thaumatin on the taste quality of processed tomatoes. Fruit of tomato cv. Yalf, transformed with the thaumatin II gene were salted and pickled and then stored for 6 months. Western blot analysis showed relative thaumatin II stability at salting; its content in processed fruits was 62-83% of the initial level depending in the studied line. In pickled tomatoes, thaumatin II content was decreased by up to 25% of the initial amount. Both salted and pickled tomatoes had a sweet taste with a typical thaumatin aftertaste. Salted tomatoes were characterized as being sweeter than pickled tomatoes. The overall taste of pickled tomatoes was rated by panellists as significantly better compared to that of salted or non-processed ones. Conclusion In the present study, we have shown that tomatoes expressing supersweet protein thaumatin II can be used for processing under mild conditions, including salting and pickling. © 2021 Society of Chemical Industry.

In the conditions of reduction of tax receivabless in a budget as a result of the crisis predefined by the pandemic of COVID-19, actual is a search of additional objects of taxation. Foreign experience shows that insalubrious foodstuffs and drinks with high maintenance of fat, sugar and salts that promote the risk of development of obesity and uninfectious diseases related to the feed can become such objects. Taxation of foodstuffs and the drinks related to the health can change food habits of population and result in reduction of level of disease. Input of excise tax on the sweet carbonated drinks, will allow to promote both fiscal and regulative role of excise taxation, as will assist reduction of consumption of this products Ukrainians. In the article the features of excise taxation are investigational in Ukraine. The necessity of study and introduction of positive experience of countries of ЄС is reasonable in relation to taxation of foods an excise tax with high maintenance of salt, sugar, caffeine and artificial substances for giving of sweet taste with the aim of upgrading of food foods and adjusting of negative influence from the consumption of insalubrious foodstuffs and drinks. Establishment of practice of taxation of soft drinks is offered in Ukraine, and also foods, that contain the high level of salt, sugar, caffeine or synthetic substances for giving of sweet taste with the aim of providing of defence of health of population, integral control after turnover of підакцизних foods, warning of avoiding tax payment. Well-proven necessity of establishment of certain list of insalubrious foods that can be used as raw material for the production of foodstuffs and drinks, with corresponding expansion and including of them to the bill of excise goods.

All sweeteners are recognized by the sweet taste receptor (T1R2-T1R3). The elucidation of the chemoreception mechanism of receptor-ligand interactions is an attractive topic for researchers. Molecular biology and computational biology techniques can reveal the proposed mechanisms for this topic. Other approaches, including chemical biology (bioorganic chemistry), have helped to identify mechanisms on the basis of molecular structure. In this mini-review, we have summarized the recent progress in the synthesis of sweetener derivatives, which includes the use of photoaffinity labeling of diazirine-based derivatives to elucidate the chemoreception of sweeteners.

O guabijuzeiro (Myrcianthes pungens) se destaca pela abrangência em todo o território nacional, pela produção e sabor adocicado dos frutos, mas poucos estudos foram conduzidos com esta espécie até o momento. O objetivo do presente trabalho foi realizar a caracterização biométrica dos frutos e análise sensorial do suco produzido de três acessos de guabiju. A metodologia utilizada consistiu na coleta dos frutos para análises de massa de frutos, rendimento de polpa, sólidos solúveis totais e análise sensorial do suco sendo realizado teste de aceitação com 35 avaliadores não treinados. Como resultados observou-se que a média da massa de frutos foi de 3,57 g, a massa de polpa foi de 1,81 g, o rendimento de polpa foi de 49,67% e os sólidos solúveis totais foi de 14,27%. Com relação a produção de suco, o acesso 1 produziu 300 mL, o acesso 2 200 mL e o acesso 3 400 mL. Com relação a análise sensorial, o acesso 1 obteve a melhor nota de aceitação por 11,42% dos avaliadores, o acesso 2 por 80% dos avaliadores e o acesso 3 por 8,57 % dos avaliadores, o que mostra o potencial de utilização destes frutos para o desenvolvimento de sucos com frutos exóticos. Pode-se concluir que existe variação entre os acessos na biometria de frutos, produção de suco e grau de aceitação dos provadores do suco de guabiju.

The human sweet taste receptor is a heterodimeric receptor composed of two distinct G-protein-coupled receptors (GPCRs), TAS1R2 and TAS1R3. The TAS1R2 and TAS1R3 subunits are members of a small family of class C GPCRs whose members share the same architecture, comprising a Venus Flytrap (VFT) module linked to the seven transmembrane domains (TMDs) by a short cysteine-rich region (CRR). The VFT module of TAS1R2 contains the primary binding site for most of the sweet-tasting compounds, including natural sugars and artificial and natural sweeteners. However, cellular assays, molecular docking and site-directed mutagenesis studies have revealed that the VFT, CRR and TMD of TAS1R3 interact with some sweeteners, including the sweet-tasting protein brazzein. The aim of this study was to better understand the contribution of TAS1R2-VFT in the binding of sweet stimuli. To achieve this, we heterologously expressed human TAS1R2-VFT (hTAS1R2-VFT) in Escherichia coli. Circular dichroism spectroscopic studies revealed that hTAS1R2-VFT was properly folded with evidence of secondary structures. Using size-exclusion chromatography coupled with light scattering, we found that hTAS1R2-VFT behaves as a monomer. Ligand binding quantified by intrinsic tryptophan fluorescence showed that hTAS1R2-VFT is capable of binding sweet stimuli with Kd values, in agreement with physiological detection. Furthermore, we investigated whether the impact of point mutations, already shown to have deleterious effects on cellular assays, could impact the ability of hTAS1R2-VFT to bind sweet ligands. As expected, the ligand affinities of hTAS1R2-VFT were drastically reduced through the introduction of single amino acid substitutions (D278A and E382A) known to abolish the response of the full-length TAS1R2/TAS1R3 receptor. This study demonstrates the feasibility of producing milligram quantities of hTAS1R2-VFT to further characterize the mechanism of binding interaction and perform structural studies.

This work investigated the effects of repeated sweet taste exposure at breakfast on perceptions and intakes of other sweet foods, while also examining the effects due to duration of exposure (1/3 weeks), test context (breakfast/lunch) and associations between taste perceptions and intakes. Using a randomised controlled parallel-group design, participants (n 54, 18 male, mean age: 23·9 (sd 5·8) years, mean BMI: 23·6 (sd 3·5) kg/m2) were randomised to consume either a sweet breakfast (cereal with sucralose) (n 27) or an equienergetic non-sweet breakfast (plain cereal) (n 27) for 3 weeks. On days 0 (baseline), 7 and 21, pleasantness, desire to eat and sweetness were rated for other sweet and non-sweet foods and sweet food consumption was assessed in an ad libitum meal at breakfast and lunch. Using intention-to-treat analyses, no statistically significant effects of exposure were found at breakfast (largest F2,104 = 1·84, P = 0·17, ηp2 = 0·03) or lunch (largest F1,52 = 1·22, P = 0·27, ηp2 = 0·02), and using Bayesian analyses, the evidence for an absence of effect in all rating measures was strong to very strong (smallest BF01 = 297·97 (BF01error = 2·68 %)). Associations between ratings of pleasantness, desire to eat and intake were found (smallest r = 0·137, P < 0·01). Effects over time regardless of exposure were also found: sugars and percentage energy consumed from sweet foods increased throughout the study (smallest (F2,104 = 4·54, P = 0·01, ηp2 = 0·08). These findings demonstrate no effects of sweet taste exposure at breakfast for 1 or 3 weeks on pleasantness, desire for, sweetness or intakes of other sweet foods in either the same (breakfast) or in a different (lunch) meal context.

Sweeteners are widely used in food products, and their sweetness potency is usually evaluated by comparing it with that of sucrose. This, however, has led to confusion as some sweeteners are evaluated based on their maximum value of sweet taste response, while others are evaluated by their threshold value. Here, we aimed to develop a novel nonverbal sweetness evaluation system through the sweet taste signal transduction by comparing the responses of the sweet taste receptor, salivation, taste intensity, and preference among six sweeteners. The hT1r2/hT1r3 sweet taste receptor responses represented the input signal of the sweet taste signal transduction, while salivation, sweet taste intensity, and participants' preferences represented the output signals by the gustatory–salivary reflex, primary gustatory cortex area, and the secondary gustatory cortex, respectively. Our results showed that the sweet taste receptor, sweet intensity, and salivary secretion responses were concentration‐dependent and expressed exponentially. Moreover, the results comparing coefficients showed 15–35 times more sensitivity between the response of hT1r2/hT1r3 and the salivation or the sweet taste intensity in non‐nutrient sweeteners. The preference graph curve was not exponential, suggesting that the sweetener preference was not related to the sweet taste receptor, salivation, or sweet taste intensity. These results may suggest that the sweet taste signal of the non‐nutritive sweeteners might be maintained by taste reception by hT1r2/hT1r3 to taste recognition in the primary gustatory area and that receptor responses and salivation could be used as indicators of sweetness intensity., Sweet intensity, and salivary secretion responses were concentration‐dependent. Sensitivity differences between taste receptor and intensity ranged from 15 to 35. Sweeteners preference is not correlated with the sweet taste receptor.

Introduction: Andrographis paniculata is medicinal plant of family Acanthaceae, consists of bitter principles and traditionally used in treatment of several gastrointestinal diseases. To mask its bitter taste, it could be formulated in honey based oral formulations like oxymel. Honey, as a saturated solution of various sugars, as per Ayurvedic system of medicine, could be consumed along with drug. Aim: This research attempt was aimed towards aqueous extraction of A. paniculata powder; formulation of oxymel by addition to honey and evaluation for different parameters. Methods: Oxymel was formulated as per procedure mentioned in United State Pharmacopeia for squill oxymel; and evaluated for pharmaceutical parameters those applied for oral syrups. Results: The oxymel formulated was dark brownish with agreeable odour and sweet taste. It was pourable with viscosity of 27.14 CP at 45.02 torque measured at 100 rpm while density was found to be 1.25 gm/ml. There was also ease in cap opening of its container, also no crystallization of honey was observed. Its andrographolide content was found to be 411.0 μg/ml. Conclusion: Bitters of A. paniculata have significant pharmacological activities in human being, if administered orally. To mask their bitter taste and facilitate their increase in absorption, A. paniculata can successfully be formulated in honey based oral formulation of oxymel.

Rice is a staple food for most Indonesian population. Nowadays, people want staple foods that are not only filling but also do not increase blood sugar concentration (low glycemic index). One of foods that contain higher starch, lower glycemic index, and higher fiber than rice is white corn. The white color of the corn is an advantage because if it is made in grits it can resemble rice. Weakness of white corn is harder than rice. To soften and make it easier to consume, it is coated with an edible packaging made from tapioca. The purposes of this study were to study (1) the quality of dried corn rice from local white corn Anoman 1 variety in edible packaging with packaging thickness of 0.7, 0.9, 1.1, 1.3, and 1.5 mm; (2) the quality of cooked white corn rice produced. The results showed that the thickness of the edible packaging significantly affected (1) the moisture, ash, protein, fat, and carbohydrate contents of dried white corn rice and (2) all parameters of cooked white corn rice (a = 0.05). The best cooked corn rice based on the stickiness test was found in coated dried corn rice with edible packaging with a thickness of 1.5 mm. Characteristics of (1) dried white corn rice contained 11.39% water, 0.21% db ash, 15.23% db protein, 0.53% db fat, 72.64% carbohydrates, 2.11% crude fiber, (2) the quality of cooked of white corn rice were 18.17 g / mm2 stickiness, yellowish color (score 1.1), slightly distinctive aroma of corn (score 2.5), slightly fluffier texture (score 3.4), and rather strong sweet taste (score 2.8). Keywords: edible packaging, local white corn Anoman 1, rice, stickiness, tapioca

El dramático aumento de la prevalencia e incidencia de la obesidad sugiere que factores ambientales y cambios en el estilo de vida contribuyen de forma importante a su tendencia epidémica. En humanos, se han reportado diferencias interindividuales en los umbrales de detección y preferencia del sabor dulce, lo que podría afectar la ingesta habitual de azúcares, y por ende al estado nutricional. Objetivo: El presente estudio busca determinar la relación entre el estado nutricional y la preferencia al sabor dulce en la comunidad de un establecimiento de educación superior. Método: Muestra fue constituida por estudiantes, funcionarios y docentes, entre 18 y 60 años, pertenecientes a la Universidad Mayor, Sede Temuco. Para determinar preferencia al sabor dulce se empleó prueba organoléptica que mide grado de satisfacción frente a solución dulce, junto a ello se realizaron mediciones de peso y talla para determinar el Índice de Masa Corporal. Resultados: Muestra final comprendió de 319 personas, de las cuales un 30,1% fueron hombres y 69,9% mujeres. No se observaron diferencias significativas en la preferencia hacia las soluciones con mayor concentración de sacarosa según el estado nutricional. Sin embargo, el modelo predictivo desarrollado arrojó que hombres prefieren las soluciones con mayor concentración de azúcar independiente de la edad y estado nutricional. Conclusiones: Es necesario desarrollar nuevos estudios que permitan aclarar si la preferencia al sabor dulce favorece el desarrollo de obesidad y sobrepeso, o si es la composición nutricional de los alimentos procesados o ultraprocesados, lo que está teniendo un mayor impacto negativo en el estado nutricional de la población. The dramatic increase in the prevalence and incidence of obesity seems to suggest that environmental factors and lifestyle changes are contributing significantly to the epidemic trend of this pathology. In humans, inter-individual differences in the thresholds of preference of sweet taste have been reported, which could affect habitual sugar intake, and therefore the nutritional status. Objective: The present study seeks to determine the relationship between nutritional status and the preference of sweet taste in the population of a higher education establishment. Method: Sample was constituted by students, officials and teachers between 18 and 60 years, belonging to the Universidad Mayor, Temuco. To determine the perception of the sweet taste, an organoleptic test was used that measures the degree of satisfaction with the sweet solution, along with this, weight and height measurements were made to determine the Body Mass Index. Results: Final sample comprised 319 people, of which 30.1% were men and 69.9% women. No significant differences were observed in the preference for solutions with a higher concentration of sucrose according to nutritional status. However, a predictive model developed showed that men prefer the solutions with the highest concentration of sugar regardless of age and nutritional status. Conclusions: It is necessary to develop new studies to clarify whether the preference for sweet taste favors the development of obesity and overweight, or if it is the nutritional composition of processed or ultraprocessed foods, which is having a greater negative impact on the nutritional status of the population.

A fabricação de queijos gera soro como subproduto, porém a maioria dos laticínios o descarta, sem o correto tratamento para este resíduo, gerando grande impacto ambiental. O doce de leite é uma sobremesa láctea de grande importância para a economia brasileira e, portanto, a utilização do soro como substituição parcial do leite é um fator preponderante no que se refere ao aumento da lucratividade dessas empresas, bem como à diminuição dos problemas ambientais relacionados. Sendo assim, o objetivo do trabalho foi compreender a influência nas características organolépticas da substituição parcial do leite integral por soro de leite em formulações de doce de leite e determinar suas características físico-químicas. Foram realizadas seis formulações com diferentes concentrações de soro, para posterior avaliação sensorial quanto aos atributos de aparência, sabor, textura, aroma e impressão global, bem como o teste do ideal para gosto doce, consistência e intenção de compra. As análises físico-químicas realizadas foram as de umidade, proteína, textura, °Brix, lipídios, colorimetria e pH. A partir da análise dos resultados, observou-se que os doces mais aceitos sensorialmente foram as formulações 3 e 4, com substituição parcial do leite por 33,33% e 41,67%, respectivamente. Diante destes resultados, podemos afirmar que a substituição parcial de leite por soro de leite é uma alternativa viável dentro do intervalo compreendido pelas amostras mais aceitas.

The estimation of strawberry hybrid forms for resistance to adverse environmental factors, productivity and consumer qualities of the fruit was carried out. Strawberry promising genotypes 35-8 (922-67×Maryshka), 928-12 (298-19-9-43×Privlekatelnaya) and 914-27 (Festivalnaya × Privlekatelnaya), which characterized by high productivity, winter hardiness, resistance to fungal pathogens and large fruit of sweet taste, were revealed.

Xylo‐oligosaccharides (XOS) are nondigestible oligosaccharides (NDO) which are recently authorized as novel food ingredients in European Union. Present study introduces the effect of XOS on baking quality of cookies. Color measurements proved that XOS enhance the caramelization during baking. Texture profile, geometry, and baking loss of cookies showed little changes due to XOS addition indicating that XOS are easy to incorporate into baking products. Based on sensory evaluation by expert panel, it was observed that XOS increased the “baked character” of the cookies as indicated by the increased caramel flavor, darker color, and crispier texture. XOS addition also increased the sweet taste and global taste intensity of cookies suggesting that in bakery products XOS evolve a flavor enhancer role. XOS proved to be a promising new alternative to increase dietary fiber content of cereal‐based cookies., Xylo‐oligosaccharides (XOS) were used for dietary fiber enrichment of cookies. Texture profile, colour and sensory attributes have been measured. It was concluded that XOS enhance brown colour, crispy texture and “baked character” of the cookies.

Nasal mucociliary clearance (NMC) system is an important defence mechanism in respiratory tract. The contents present in the smoke are found to be toxic to cilia in vitro. Our study aims at evaluating the NMC in smokers so as to know effect of tobacco smoke on ciliary function and also comparing it with non smokers. We also studied effect of duration, intensity and pattern of smoking on the NMC. A total of 60 participants in the age group 21–40 years (30 smokers and 30 non smokers) were included in the study. Saccharin test was performed in all participants. A 0.5 mm diameter particle of saccharin was placed 1 cm from the anterior end of the inferior nasal turbinate. The time duration was noted for the first appearance of sweet taste. The mean NMC in smoker group was 16.53 min and in nonsmoker group was 9.28 min On comparison it was found that NMC time in smoker group was significantly higher than nonsmoker group p

Desiccated coconut is one of the diversification of coconut products which is cut into pieces or grated into small pieces and dried, white, sweet taste and distinctive odor. The purpose of this study was to determine the drying characteristics of desiccated coconut using a cylindrical dryer. Eight trays, each containing 350 grams of grated coconut, were arranged on a tray and measured the air temperature in the drying chamber, the temperature of the material, as well as the moisture content of the material during drying both on the top rack, middle rack and bottom rack. The results showed that the air temperature in the drying chamber and the temperature of the material were significant, and moisture content during drying of materials both on the top rack, middle rack and bottom are relatively significant. The constant rate of drying of grated coconut on the upper rack, middle rack and lower rack is 0.1306 - 1338 minutes-1. The predicted value of grated coconut water content shows that it is almost close to the observed water content value, which is marked by a coefficient of determination that is close to 1.

Although sugar intake did not directly lead to diabetes, the results will vary depending on age, sex, individual differences, and the nature of sugar ingested.However, the change in blood glucose level and the accumulation of fat in the body cannot neglected about the sugar intake. Also, if the sweetness recognition threshold increases, the intake of sugar may increase. Therefore, the purpose of this study was to conduct a sweetness cognitive threshold test to understand the sensitivity of the general public to sweetness. The acceptable range (sugar concentration of 2.5% or less) was 25 out of 38 participants who recognized sweetness, 65.8% of the total. Two of the 14 male participants were unrecognizable even at the sweetest concentration of 80.0%. Females had better sweetness perception threshold results than males. In the future, we think it would be good to use a questionnaire to investigate the usual eating habits and compare it with the wetness cognitive threshold test results.

Background/Aim: Individuals make food choices based on a number of physiological, nutritional, environmental and socioeconomic factors but sensory qualities of food namely the taste has priority as the determinant in food selection. The purpose of the study is to evaluate sweet, salty, sour and bitter taste perceptions of school children and compare them in term of caries experience. Material and Methods: Two hundred children aged from 6-13 were included in the study. The dental examinations of children were performed using DMF(T), DMF(S), df(t) and df(s) indices. Questionnaires were presented to parents to record the socioeconomic and educational levels, oral health knowledge, child's general health, oral health habits and fluoride exposure. Children rinsed sucrose (12g/L; 24g/L), sodium chloride (2g/L; 4g/L), citric acid (0,6g/L; 1,20g/L) and caffeine (0,27g/L; 0,54g/L) solutions randomly and the taste perception was recorded. Relationship between the taste perception and caries experience were evaluated. Results: A significant correlation was found between 2 g/L of NaCl and age (p= 0,007, r= 0,178). When occlusal or approximal caries of the children aged from 6 to 9 were discriminated, the Spearman's test found a weak positive correlation between occlusal caries and higher sweet taste (24 g/L sucrose) (r= 0,232; p= 0,021) and a weak negative correlation between approximal caries and higher salty taste (4 g/L NaCl) (r= -0,225; p= 0,025). Conclusions: Age should be considered in the assessment of taste perception of children. Additionally, there is a weak relationship between taste perceptions and dental caries. These data suggest that further studies need to focus on the effect of taste preferences on dental caries.

This study compared the physicochemical properties and sensory quality of fresh lotus seeds of ‘Mantianxing’ and ‘No.36 space lotus’. The results showed that along with the maturation of seeds, the seed shell color changed from green to brown and that of the plumule turned from yellow to green. In addition, the moisture content decreased, the hardness significantly increased, and the plumpness remained constant. The above changes occurred 4 days earlier in August than in September. During maturation, the total phenol content and DPPH free radical scavenging capacity decreased; the soluble sugar content and sweet taste first increased and then decreased; and the alkaloid content and bitter taste showed an overall increasing trend. Hence, the quality of fresh lotus seeds is determined by the harvesting time, and lotus seeds harvested on day 14 in August and day 18 in September have the highest fresh quality.

Sweetener as a food additive which gives sweet taste has a different profile from sucrose. Intensity profile and attribute sensation become very useful in developing mixed sweeteners from single sweeteners to create commercial sweeteners having a sucrose-like profile. However, there is only few data of attribute intensity and descriptive profiles of single sweeteners. The aim of this research was to analyze the des-criptive profile of single and mixed sweeteners using quantitative descriptive analysis (QDA), time intensity (TI), and temporal dominance of sensations (TDS) methods. The results of QDA descriptive profile showed that samples of a single sweetener T3 as well as mixed sweetener containing C2 and C5 had the same characteristics as sucrose. The results of TI descriptive profiles showed that the time intensity curves of sweet attribute of a sample of two mixed sweeteners C1 and C2, and a single sweetener T3 were similar to that of sucrose. The results of the descriptive profile using TDS methods generally showed that the do-minant attributes of all samples were sweet, sweet aftertaste, and licorice. Bitter and bitter aftertaste attri-butes were found in single sweetener T7, while mixed sweetener C1 had best sensory characteristics than the others.

We hypothesized that subjects with genetic variants that increase sweet taste preference would consume more sucrose‐containing foods and have altered energy and glucose metabolisms, which would have interactions with lifestyles. Korean genome and epidemiology study (KoGES) was conducted to determine genetic variants and lifestyles including nutrient intakes by the Korean Center for Disease and Control during 2004–2013. Subjects were 8,842 adults aged 40–69 years in Ansan/Ansung cohorts in Korea. The associations between genetic risk scores(GRS) selected for influencing higher sweet preference and energy and glucose metabolism were examined using logistic regression after adjusting for covariates. GRS included 8 SNPs, TAS1R2_rs61761364, SLC2A5_rs11121306, SLC2A7_ rs769902, SLC2A5_rs765618, TRPM5_rs1965606, TRPV1_rs224495, TRPV1_ rs8065080, and TRPV1_rs8078502. Sweet taste preference was higher by 1.30‐folds in high GRS than in low GRS (p, Eight genetic variants associated with sweet taste preference were selected in the genes involved in taste receptor signaling. Adults carrying genetically high sweet taste preferences were associated with consuming higher sucrose‐containing food intakes and improving glucose tolerance. High mental stress and lack of physical activity quenched the genetic impact.

Whey dangke is a byproduct of the dangke industry and has not been utilized. The application of whey dangke to be a fermented beverage will provide functional and economical use values. Fermented whey products need to study the use of sugar in connection with future consumer preferences. Sucrose can be metabolized by microorganisms. The use of sucrose and the activity of microorganisms during fermentation can influence the sensory assessment of the final product of whey fermentation. The purpose of this research was to determine the sensory physical characteristics of fermented whey beverage products by adding various percentages of sucrose. The treatment in this research was the use of sucrose percentages of 3, 6, 9, and 12 % (w/v) and without the addition of sucrose and also using commercial products as a comparison. Sensory testing used 36 semi-trained panelists. Sensory assessment included sour taste, sweet taste, panelists' preference for aroma and appearance, and responsiveness of reception from panelists. The results showed that an increase in the percentage of sucrose causing whey fermentation of sour taste decreased, while the sweet taste, aroma and appearance preference increased. The panelist acceptance response to the sour and sweet taste of the fermented whey beverage product was the response received, while the panelist acceptance response to the aroma and appearance was from a neutral response to receiving. The used of 12% sucrose percentage in the processing of fermented whey drinks would produce drinks that are preferred by consumers. Keywords : whey dangke, fermentation, sucrose, sensory characteristics

Neoculin is a sweet taste protein capable of modifying sour taste into sweet taste. Neoculin, along with other sweeteners, are received by the human sweet taste receptors T1R2 and T1R3. To date, there has been few studies regarding how neoculin interacts with the human sweet taste receptors in molecular level. In this study, computational approaches were applied to elucidate how neoculin interact with T1R2 and T1R3 at molecular level. In order to achieve this research, homology modeling for T1R2 and T1R3 was performed to predict their structure. A protein-protein docking study was conducted between neoculin and T1R2 and T1R3, which displayed a strong relationship with the previous experimental findings regarding the important residues of neoculin, and how they interact with the ATD domain of T1R3. These residues are His11, Asp91, Tyr21, Asn44, Arg48, Tyr 65, Val72, and Phe94. The best docked complexes were then subjected to molecular dynamics simulation for further analysis. The molecular dynamics simulation results showed the contributions of the important residues of neoculin in forming hydrogen bonds with the residues of the receptors. The binding energy between neoculin and each of T1R2 and T1R3 were also calculated. These results concluded that neoculin sweet taste and taste modifying abilities are only active when it binds to the amino terminal domain of T1R3.

Increased urbanization created lack of authentic and genuine drug for the management of ailments of human beings; similarly the urbanization has fashioned myths in society that lactation and feedings of the babies leads to loss of beauty, along with this stress, strain and modern style of living affects the milk production in human being. Galactogouges are most used and prescribed drugs in the medical practice. As per Ayurveda galactogouges are termed as stanyajanana dravya, which increase the milk production. Karpasa beeja (Gossypium herbacum Linn) belongs to Malvaceae family is medium sized tree consists madhura rasa (sweet taste), sheeta veerya (cold potency), and madhura vipaka (under goes sweet metabolism). It acts as vata, pitta shamaka, kapha vardhaka, and stanyajanana (increases lactation). The Karpasa beeja was subjected for morphological and physico-chemical evaluation according to the parameters explained in Ayurveda Pharmacopeia of India and galactogogue activity was carried out for 15 days by using 24 Albino rats divided into four groups i.e. two trial groups (Churna and Extract of Karpas Beeja), one standard group (Shatavari churna) and one control group (Normal saline water). The drug shows presence of carbohydrate, proteins, sterols, reducing sugar, tannins, flavonoids, alkaloids. The drug in the form of churna and 90% Ethyl alcohol extract shows similar effects with known standard drug Shatavari (Asparagus racemosa).

A case-controlled study was performed to evaluate taste and smell impairment, nausea or vomiting (NV) response to taste and smell and toleration to food texture, item and cooking method in hyperemesis gravidarum patients (HG) compared to gestation-matched controls from a university hospital and primary care clinic in Malaysia. Taste strips (4 base tastes), sniff sticks (16 selected smells) and a food-related questionnaire were used. 124 participants were recruited. Taste impairment was found in 13%(8/62) vs. 0%(0/62) P = 0.003 and the median for correct smell identification was 5[4–6] vs. 9[7–9] P