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Introdution: Vitality of periodontal ligament (PDL) cells is very critical for replantation of complete avulsion teeth due to traumatic injuries. This is important for transferring an avulsed tooth to clinic for replantation that which Medias used for storage.This study aimed to compare the vitality of PDL cells of sheep teeth cultured in different storage medias including α-Minimum Essential Medium (αMEM), Dulbecco’s Modified Eagle’s Medium (DMEM), Hank’s Balanced Salt Solution (HBSS), and mint extract Methods: In this lab trial study, PDL cells were obtained from 124 healthy anterior and posterior sheep teeth and cultured in αMEM, DMEM, HBSS, and mint extract for periods of 2, 6, 24, 48, 72, or 96 hours (24 groups). For each solution, positive control group were PDL cells without incubation in any storage media. For each group, there was a negative control considered cells growing in dry plate with no medium. After exposure of PDL cells to scheduled solution for scheduled incubation time, centrifuge was performed for 10 minutes at rate of 2000G. Then cell percipitates were added into the solution of collagenase (3mgr/ml) and Dispase (4mgr/ml to cell precipitates, which were incubated at 37° C for 60 minutes. After washing cellular suspension in PBS, vitality of the cells was assessed by Trypan blue exclusion, on a neobar slide by magnification of 200X. The data were analyzed statistically using 2-way ANOVA test by SPSS version 15. Results: Statistically significant differences in efficacy of different medias were obtained at least between two media (P=0.0001). PDL cells cultured in αMEM and mint extract showed 90% and 52.22% vitality representing, respectively, the best and the weakest storage media. Conclusion: αMEM can be a suitable transport medium up to 96 hours to preserve the vitality of the PDL cells of avulsed teeth. There is a reverse correlation between the viability of PDL cells and incubation time, increasing the time decreases the viability.

Objective: To compare the efficacy of platelet-rich fibrin (PRF) and Hank’s balanced salt solution (HBSS) in the preservation of the periodontal ligament (PDL) cells viability of avulsed teeth. Methods: A total of 30 non-carious third molars with healthy periodontium, indicated for extraction for orthodontic reasons or chronic pericoronitis, were selected for the study. Samples were divided into four groups: one standard group and one experimental group in addition to two control groups (positive and negative). The positive and negative control group corresponded to immediate and 2-hour dry time respectively. The experimental teeth were bench dried for 40 minutes and then immersed in one of the two storage media: HBSS (standard storage media) and PRF (experimental storage media) for 45 minutes. The teeth in each group were treated with dispase II and collagenase for 30 minutes and later centrifuged for 4 minutes at 1000 rpm. The supernatant was removed with sterile micropipette, the cells were labeled with 0.4% trypan blue, and the number of viable PDL cells was counted with a hemocytometer under a light microscope. One-way Kruskal–Wallis test and Mann–Whitney U test with Boneferroni correction were used for statistical analysis. Results: Results did not demonstrate any statistically significant differences in the viability of PDL cells between the groups with standard and experimental storage media. Group 1 showed a statistically significant difference of mean compared to Groups 2, 3 and 4. When Group 2 was compared with Groups 3 and 4, a P-value>0.05 suggested no statistical significance. Conclusion: Within the parameters of this study, HBSS and PRF demonstrated a similar number of viable PDL cells. Hence, PRF could be a used as a good substitute of HBSS as a storage media for avulsed teeth.

Introduction: Various storage media have been investigated due to their ability to maintain the viability of the Periodontal Ligament (PDL) cells, thus permitting longer extra-alveolar periods prior to replantation of avulsed teeth. Aim: To assess the efficacy of a newer storage medium, Aloe Vera Gel in comparison with milk and Hank’s Balanced Salt Solution (HBSS) in maintaining the viability of PDL cells in simulated avulsed teeth. Materials and Methods: The present in-vitro study was performed on 40 freshly extracted human premolars with intact crown and closed apices, extracted for orthodontic reasons. They were randomly assigned to one of the three storage medium groups (N=10 each) and a positive and negative control (N=5 each). The positive and negative controls corresponded to 0 minute and an eight hours dry time respectively. The teeth in the experimental group were dried at room temperature for 30 minutes followed by 45 minutes immersion in one of the three experimental media. After drying and soaking, the root surface of each tooth was treated with an enzymatic solution containing 1 mL of Collagenase Type II and Dispase Type I in phosphate buffer saline (PBS). The cells were then labelled with 0.4% Trypan blue and counted using a haemocytometer under a light microscope at 10x magnification. Kruskal Wallis H-test was used to analyse the mean difference in the number of viable cells among the five groups. Results: The teeth stored in HBSS demonstrated the highest number of viable PDL cells followed in rank order by milk, positive control, aloe vera and negative control. There was no significant difference in the number of viable PDL cells between HBSS and milk. Conclusion: From the results of the present study it can be concluded that aloe vera may be better than negative control to maintain PDL cell viability but not more than HBSS and milk.

Данная статья посвящена югоностальгии – тоске по Социалистической Федеративной Республике Югославии (СФРЮ) – одному из наиболее характерных и популярных явлений, появившихся на почве постюгославских культур. Этот феномен показан через призму кулинарии, которая рассматривается как носитель автобиографической и коллективной памяти, медиум, передающий образы прошлого. Проанализированы четыре книги, в которых в качестве структурообразующего элемента использованы формы лексикона – Leksikon Yu mitologije, Bila jednom jedna zemlja. Titova Jugoslavija (Лексикон Ю мифологии, Была когда-то страна. Югославия Тито) – и кулинарной книги – Titov kuvar, Emigrantski kuvar (Поваренная книга Тито, Эмигрантская поваренная книга). Собранные в них тексты, посвященные кулинарии, тесно связаны с автобиографическим и биографическим повествованием. Описываемые блюда и продукты питания призваны реконструировать прошлое, позволяют вернуться во времена и места, вызывающие тоску по утраченному дому. Продиктованное маркетинговыми целями соединение (авто)биографического повествования со структурой кулинарных книг показывает также другой образ югоностальгии, которая оказывается отлично продаваемым товаром., This article focuses on Yugo-nostalgia - yearning for the Socialist Federal Republic of Yugoslavia (SFRJ) - one of the most characteristic and popular phenomenon observed on the basis of post-Slavic cultures. This phenomenon was shown through the culinary prism, which was treated as the carriers of autobiographical and collective memory mediating the transmission of images of the past. Four books were 88 analyzed, in which a lexicon - Leksikon Yu mitologije, Bila jednom jedna zemlja. Titova Jugoslavija (Lexicon of Yu Mythology, There was once a country. Tito’s Yugoslavia) and a cookbook - Titov kuvar, Emigrantski kuvar (Tito’s Cookbook and The Immigrant’s Cookbook) were used as a construction scheme. The texts collected there are closely connected with biographical and autobiographical narrations. Particular dishes and foodstuffs participate in the process of reconstruction of the past, allow to return to times and places soothing nostalgia for the lost home. Due to marketing reasons, the combination of narrative (auto)biographical and culinary reveals the second face of Yugo-nostalgia, which is an attractive product and sells well.

—: The aim of this study was to compare the effect of different immersion protocols into various acidic media on the surface roughness of various glass ionomer-based dental restorative materials in vitro. The total number of 200 specimens were prepared from 5 different restorative material groups. Ten specimens from each group were then immersed into one of the tested beverages including; Coca-Cola, Iced-Tea, orange juice and distilled water (control). All specimens were kept in oven at 37°C for designated time periods of 1 day, 1 week, 1 month, 3 months, 6 months and 1 year and solutions were renewed weekly. Surface roughness measurements were performed at baseline and after each immersion period. The surface roughness of all glass ionomer-based restorative materials were affected by immersion with acidic media to a degree. For Glass Carbomer, there were steady increases in surface roughness in Ice Tea and orange juice (p < 0.05). The only significant increase in surface roughness of Fuji IX was found in orange juice (p < 0.05). However, the difference was not significant in coke and ice tea in comparison to distilled water (p > 0.05). When compared with distilled water, surface roughness of Chemfil Rock had increased significantly in coke and orange juice (p

The importance of the process of restoring a database requires precise examination of all factors that affect it. Considering the decline of the price of SSD and its better read and write speeds it seems that the logical choice when reducing downtime is primary concern for the organisation. This paper categorizes storage devices in use as well as the main backup types in the MS SQL Server. Tests are conducted to evaluate the impact of the storage device on backup and restore efficiency. In this regard, the purpose of the research is to examine problems associated with the selection of a storage device for efficient backup and restore in MS SQL Server. Conducted tests showed some surprising results regarding SSD’s efficiency. The reason these findings are important is that they could be used as a guideline for choosing proper storage device for an organisation’s needs. ACM Computing Classification System (1998): H.2.7, I.3.1, D.4.2. Mathematics Subject Classification – MSC2020: 68P15.

The aim of this study was to evaluate the viability of periodontal ligament cells of avulsed teeth in three different storage media. Forty-five mature premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups according to the storage medium: milk (control), rice water and egg white. After placing extracted teeth for 30 min in storage media, the scrapings of the periodontal ligament (PDL) were collected in Falcon tubes containing collagenase in 2.5 mL of phosphate buffer saline and were incubated for 30 min and centrifuged for 5 min at 800 rpm. Cell viability was analyzed by Trypan blue exclusion. Rice water had a significantly higher number of viable cells compared to egg white and milk. There was no statistically significant difference between egg white and milk. Rice water may be able to maintain PDL cell viability of avulsed teeth better than egg white or milk. Resumo O objetivo deste estudo foi avaliar a viabilidade das células do ligamento periodontal de dentes avulsionados armazenados em três diferentes meios. Quarenta e cinco pré-molares com formação radicular completa extraídos por razões terapêuticas foram aleatoriamente distribuídos em três grupos, de acordo com o meio de armazenagem: leite (controle), água de arroz e clara de ovo). Após armazenar os dentes avulsionados por 30 min no meio, raspas do ligamento periodontal (LPD) foram coletadas em tubos Falcon contendo 2,5 mL de solução tamponada de soro fosfatado, incubadas por 30 min e a seguir centrifugadas por 5 min a 800 rpm. A viabilidade celular foi analisada pelo método de exclusão do Azul de Trypan. A água de arroz teve um número significativamente maior de células viáveis em comparação com o leite e a clara de ovo. Não houve diferença estatisticamente significativa entre o leite e a clara de ovo. A água de arroz pode ser capaz de manter a viabilidade das células do PDL de dentes avulsionados, melhor que o leite ou a clara de ovo.

Statement of Problem: Accumulation of plaque and staining due to a rough surface, and penetration of colourant agents from food and beverages in to the resin composite results in an incomplete polymerization. There is a little information on the effect of finishing and polishing techniques on the discoloration of nanohybrid and microhybrid composites when exposed to staining solutions. Objectives: To determine the degree of surface staining of nanohybrid and microhybrid composites after polishing and immersion in distilled water and two commonly used staining solutions. Materials and Methods: A nanohybrid (Ice; SDI) and microhybrid (Gradis direct; GC) composites were used. Disc-shaped specimens were prepared and treated with either a matrix finish or polished using Sof-Lex discs (3M/ ESPE) and Enhance point (Dentsply). After 24 h immersion in distilled water at 37°C the specimens were polished and colour coefficients (CIE L* a* b*) was measured by a spectrophotometer. All specimens were immersed in 37°C distilled water in an incubator for 7 days and colour coefficients were measured again. The colour change (ΔE) was calculated using the following formula: ΔE = [(Δa)2+(Δb)2+(ΔL)2] 1/2. The data were analyzed using three-way ANOVA, one-way ANOVA/Tukey HSD and Student’s t-test. Results: There was a significant interaction between resin composites, polishing systems and staining solutions (p < 0. 05). ANOVA and Tukey’s tests showed that Ice had a significantly lesser colour change than Gradia direct and matrix finish revealed the smoothest surface followed by Sof-Lex discs and Enhance point. Distilled water and cola caused no perceptible colour change (ΔE < 3.3). The effect of surface polish on staining was statistically significant (p < 0 .05). Conclusions: Sof-Lex discs in comparison to Enhance point stimulated greater staining resistance for both composites. The nanohybrid exhibited less colour change than microhybrid composite. Coffee was the only storage media that induced a perceptible colour change (ΔE > 3.3) compared to cola and distilled water.

Context: Avulsion injury is a severe form of dental trauma. Due to the complexity of this injury, the neurovascular supply is severely compromised and usually results in the loss of pulp vitality. A variety of factors can influence the success of replantation especially the storage media and storage period. Casein phosphopeptides are derived from casein which accounts for 80% of the total protein in bovine milk. They can function as carriers for several minerals especially calcium and also have known to have antibacterial properties. Aim: The purpose of this study is to evaluate the efficacy of GC Tooth Mousse Plus in comparison with chilled Homogenous pasteurized whole milk as a storage media. Materials and Methods: Thirty healthy premolars that are extracted for orthodontic purpose will be divided into three groups: (1) Group A: Control (Saline), (2) Group B: Positive control (Chilled Homogenous milk), (3) Group C: Experimental (GC Tooth Mouse Plus). These groups were further divided into two subgroups, each indicating storage period of 30 min and 60 min. The periodontal ligament (PDL) cell viability will be checked using tryphan blue staining. Results: Data were analyzed using Student's t-test. Conclusion: GC Tooth Mousse Plus can be used as a storage media to prevent desiccation of PDL cells up to the duration of 60 min.

WOS: 000380264700002 PubMed: 26635107 Background and aimThe purpose of this study was to evaluate the effectiveness of goat milk as a storage media for maintenance of periodontal ligament (PDL) cell viability of avulsed teeth and compare it with commonly used and/or investigated storage media. Materials and methodsPDL cells were obtained from the root surface of healthy premolars and were cultured in Eagle's maintenance medium (EMM). Cell cultures were treated with the following storage media: tap water (negative control); EMM (positive control); Hank's balanced salt solution; ultra high temperature (UHT) long-shelf-life lactose-free cow milk; UHT long-shelf-life whole cow milk; UHT long-shelf-life skimmed cow milk; UHT long-shelf-life soy milk; UHT long-shelf-life goat milk, UHT long-shelf-life follow on milk with probiotic, 20% propolis, and egg white. Culture plates were incubated with experimental media at 20 degrees C for 1, 3, 6, 12, and 24 h. PDL cell viability was assessed by tetrazolium salt-based colorimetric (MTT) assay at each test period. One-way anova was used to evaluate the effects of storage solutions at each time point, followed by post hoc Duncan's multiple comparison test (P = 0.05). A dendrogram was constructed to show the arrangement of hierarchical clustering. ResultsGoat milk displayed the highest capacity to maintain cell viability at all test intervals (P < 0.001). Between 3 and 24 h, milk with the probiotic showed the lowest time-dependent PDL cell viability among all test media (P < 0.001). Compared with all milks, HBSS performed significantly less effectively in maintaining PDL cell viability during the entire test period (P < 0.001). ConclusionBased on PDL viability, goat milk can be recommended as a suitable storage medium for avulsed teeth. Ondokuz Mayis University of Samsun, TurkeyOndokuz Mayis University [PYO.DIS.1901.14.004] This study was supported by Ondokuz Mayis University of Samsun, Turkey, vote PYO.DIS.1901.14.004.

Handout on storage media durability., An overview of properties, advantages and risks of storage media devices., {"references":["Gilbert, M. W. (1998). Digital media life expectancy and care. University of Massachusetts Amherst Office of Information Technologies Newsletter."]}

Apiterapija je vrsta znanosti i umjetnosti koja se bavi održavanjem, prolongiranjem i čuvanjem zdravlja koristeći se proizvodima pčela medarica (Apis mellifera) kao što su med, propolis, matična mliječ, pčelinji otrov i pelud. Med po kemijskoj građi predstavlja složenu smjesu koja se sastoji od ugljikohidrata, minerala, vitamina, aminokiselina i bioaktivnih tvari poput fenolne kiseline, flavonida i antioksidansa. Topikalna aplikacija meda pozitivno modificira PH vrijednost zubnog plaka, smanjuje broj bakterija u zubnom plaku i usporava njihov razvoj. Dokazan je pozitivan učinak meda u terapiji alveolarnog osteitisa i prevenciji nastanka radijacijskog mukozitisa. Propolis je zeleno - smeđa, tvrda i čvrsta materija, kruta na sobnoj temperaturi. Zbog protuupalnog i antimikrobnog djelovanja i zbog inercije prema stanicama parodontog ligamenta, propolis se može koristiti kao siguran i učinkovit intrakanalni medikament. Matična mliječ je jedan od proizvoda pčela medarica koji ima brojne potencijale u liječenju bolesti. Dokazano je odličan medij za pohranu avulziranih zuba zbog održavanja stanica parodontnog ligamenta u životub, Apitherapy is a science that deals with the maintenance, prolonging and preservation of health, using the Apis mellifera products, such as honey, propolis, royal jelly, bee poison and pollen. Honey is a chemical compound consisting of carbohydrates, minerals, vitamins, amino acids and bioactive substances such as phenolic acids, flavonoids and antioxidants. The topical application of honey positively changes the pH of the plaque, reduces the number of bacteria in the plaque and reduces their development. The positive effect of honey in the treatment of alveolar osteitis and the prevention of oral mucositis has been demonstrated. Propolis is green – brown material solid at room temperature. Propolis can be used as a effective root canal medicament due to his anti-inflammatory and antimicrobial effects. Royal jelly is one of the bee products, which has many curing potentials. Royal jelly demonstrates beneficial properties as a potential tooth storage medium.

V današnjem hitrem tempu je varnostno kopiranje podatkov izredno pomembno. Običajno se podatkovne datoteke hranijo na eno mesto, kar je zelo nevarno. Računalniške izgube, kraje, naravne nesreče in nenamerno brisanje so samo nekateri načini, zaradi katerih lahko izgubimo podatke. Zato je izredno pomembno, da ima podjetje kakovostno varnostno strategijo. Obstaja veliko varnostnih načinov za varnostno kopiranje podatkov, ki zagotavlja zmanjšano tveganje izgube le-teh. V diplomskem delu smo predstavili pomen varnostnega kopiranja podatkov in podjetju Pancom, d. o. o., predlagali rešitev z uvedbo varnostnega kopiranja podatkov prek mrežnega diska NAS. Za nadaljnji razvoj informacijske tehnologije podjetja je nujna skrb za redno posodabljanje programske opreme in redne strojne nadgradnje. S tem ima podjetje na voljo več programskih funkcij, ki podjetju omogoči nemoteno poslovanje, varno kopiranje podatkov na strežnik, nižje stroške poslovanja ter čas. In today's fast pace, data backup is extremely important. Usually, data files are stored in one place, which is very dangerous. Computer losses, thefts, natural disasters and inadvertent deletion are just some of the ways we can lose data. It is therefore extremely important that the company has a high-quality security strategy. There are many security ways to back up data, which will surely reduce the risk of losing them. In the diploma paper we presented the importance of data backup and the company Pancom, d. o. o., propose a solution by introducing backup of data over the NAS network grid. In order to further develop the company's information technology, it is essential to regularly update the software and regular hardware upgrades. This will offer several software features, which will enable the company to operate smoothly, securely copy data to the server, lower operating costs and time.

Background/Aim: Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators’ effects on viability or differentiation of dental stem cells. The aim of this study was to investigate the effect of Ectoine as a storage media on the viability and differentiation potential of human periodontal ligament mesenchymal stem cells (hPDLMSCs). Materials and Methods: hPDLMSCs were obtained from impacted third molar teeth. The cells were isolated, submitted to trilineage differentiation, and characterized by flow cytometer (FC). hPDLMSCs were incubated with different media containing Ectoine, complete DMEM (cDMEM), Ectoine+cDMEM, milk, and tap water for 2, 6, 12, and 24 h. The cells were analyzed by FC for viability, early-apoptosis, late apoptosis, and necrosis rates. Osteogenic and fibroblastic differentiation in hPDLMSCs were investigated by Alizarin red stain and vimentin expression. Results: All treated groups showed significant decreases in cell viability after 2 h. Significant increases were detected in the number of dead cells between 2 and 12 h in all groups except the Ectoine+cDMEM group. The deposition of mineral matrix nodules was significantly higher in cells cultured with Ectoine+cDMEM compared with the other media. Higher vimentin expressions were detected in cells cultured with cDMEM and Ectoine+cDMEM media, respectively. Conclusions: Ectoine added to cDMEM media, promoted cell survival plus osteogenic and fibroblastic differentiation of hPDLMSCs.