1,482 results on '"Sousa, Carla"'
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152. Necessidades não satisfeitas em saúde e estratégias de financiamento dos custos de saúde das famílias em Portugal
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Sousa, Carla Cristina Silva e, Veiga, Paula, and Universidade do Minho
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Necessidades não satisfeitas ,Out of pocket health expenditures ,Health ,NHS ,Health expenditures ,Despesas de saúde ,Ciências Sociais::Economia e Gestão ,SNS ,Financiamento das despesas diretas de saúde ,Unmet needs - Abstract
Dissertação de mestrado em Economia Monetária, Bancária e Financeira, O estudo tem como objetivo uma análise sobre as Necessidades de Saúde não satisfeitas e as estratégias de financiamento dos gastos de saúde diretos pelas famílias em Portugal. Apesar de o Serviço Nacional de Saúde oferecer cobertura universal e uma vasta gama de benefícios, a literatura sugere que persistem dificuldades e desigualdades no acesso e no financiamento dos gastos de saúde em Portugal. Dois indicadores fundamentais para avaliar as dificuldades no acesso e as desigualdades em saúde são as necessidades não satisfeitas da população e a proporção dos gastos diretos em saúde. Dado o elevado custo de oportunidade dos gastos de saúde, importa também conhecer como é que as famílias lidam com os gastos diretos. O estudo contou com a divulgação de um inquérito a adultos residentes em Portugal, respondido eletronicamente, ao qual foram obtidas 284 respostas. Todos os questionários foram considerados válidos. Trata-se de um estudo exploratório, sendo privilegiada a análise descritiva dos resultados. Os resultados estão em linha com estudos anteriores que sugerem uma elevada percentagem de necessidades não satisfeitas em Portugal, em particular no que concerne a consultas de especialidade e cuidados primários. As necessidades não satisfeitas estão em particular associadas a elevados tempos de espera e às dificuldades de suportar os custos. Indivíduos de agregados com maiores dificuldades financeiras e com prevalência de doença reportam mais frequentemente necessidades não satisfeitas. As conclusões estão em linha com as de estudos anteriores. O estudo indica os gastos em saúde, têm um peso elevado no seu rendimento. Os resultados indicam ainda que os indivíduos recorrem principalmente a poupanças para custear as despesas. O recurso ao crédito bancário é pouco usado. Uma importante limitação é que a amostra do estudo é pequena e não probabilística. O método de administração dos questionários resultou numa amostra que diverge das características médias da população portuguesa., The study aims to analyse the unmet health needs of Portuguese, and their strategies for financing direct health expenditures. Although the National Health Service offers universal coverage and a wide range of benefits, the literature suggests that difficulties and inequalities persist in accessing and financing health expenditures in Portugal. Two fundamental indicators to assess difficulties in access and inequalities in health are the population's unmet needs and the proportion of out-of pocket health expenditures. Given the high opportunity cost of health care expenditures, it is also important to know how families manage to pay the out-of-pocket health expenditures. The study included the disclosure of a survey to adults residing in Portugal, answered electronically, to which 284 responses were obtained. All surveys were considered valid. This is an exploratory study, with a descriptive analysis of the results. The results are in line with previous studies that suggest a high percentage of unmet needs in Portugal, in particular regarding specialty consultations and primary care. Unmet needs are main due to high waiting times and difficulties in bearing costs. Individuals from households with greater financial difficulties and with prevalence of disease more often report unmet needs. The conclusions are in line with those of previous studies. The study indicates that health spending has a high weight in their income. The results also indicate that individuals mainly use savings to cover expenses. The use of bank credit is little used. A main limitation is that the study sample is small and non-probabilistic. The questionnaire administration method resulted in a sample that deviates from the average characteristics of the Portuguese population.
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- 2023
153. Additional file 1 of Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
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da Moura, Aires Januário Fernandes, Valadas, Vera, Da Veiga Leal, Silvania, Montalvo Sabino, Eddyson, Sousa, Carla A., and Pinto, João
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Additional file 1: Table S1. Primer sequences used for molecular identification of mosquito species collected in Cape Verde islands. Table S2. Primers used for PCR detection of Wolbachia and genotyping of wPip I–V groups by PCR-RFLP. Table S3. Primers used for Wolbachia MLST loci and wsp hypervariable region amplification and sequence analysis.
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- 2023
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154. Additional file 2 of Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
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da Moura, Aires Januário Fernandes, Valadas, Vera, Da Veiga Leal, Silvania, Montalvo Sabino, Eddyson, Sousa, Carla A., and Pinto, João
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Additional file 2: Table S4. Mosquito species collected on each island and tested for Wolbachia using wsp.
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- 2023
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155. Game-based learning in higher education using analogue games
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Maratou, Vicky, Ennami, Firdaous, Luz, Filipe, Abdullahi, Yama, Medeišienė, Raimonda Agnė, Ščiukauskė, Indrė, Chaliampalias, Rizos, Kameas, Achilles, Sousa, Carla, and Rye, Sara
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analogue games ,learning ,game-based learning ,education ,serious gaming - Abstract
Games have been effective in helping people to interact with one another and learning more about the culture they inhabit (Piaget, 1962). The importance of games and their centrality to culture is pointed out by Huizinga (1944) who suggested using them as a medium to organise our lived experience and as an escape from their pragmatic focus (Ruckenstein, 1992). The “playful” nature of games results in escapism because it often hides the seriousness of their outcomes (Bateson, 2014; Henricks, 2006). For instance, games can be implemented in war where they are viewed as a deadly sort of game, with elaborate rules, strategies, and codes of sportsmanship. Furthermore, the widespread use of games has gained traction as a rapidly evolving teaching and learning tool in the educational sector as well. This rapid growth in the use of games as an educational tool has led to the creation of an immense number of diverse games, aiding teaching and learning in a multitude of disciplines from economics to art, and numerous encyclopaedic websites of previously developed games have been created. Despite this rapid growth, it is debatable whether this is due to increased effective learning from games, or simply the increased engagement and enjoyment observed in comparison to traditional pedagogical methods. To that end, the current paper explores the practices of analogue GBL across European Higher Education Institutions (HEIs) and the challenges and opportunities associated with Game-Based Learning (GBL) from the perspective of educators and game designers. Gamification – including simple game mechanics, such as points for correct answers (Hidi & Renninger, 2006; Kim, Song, Lockee, & Burton, 2018; Rotgans & Schmidt, 2011) – is often used as a teaching aid to increase student engagement and enjoyment. However, simply adding a game mechanic into classic, lecture-based teaching does not necessarily assist in cognitive retention in classrooms, or develop 21st-century skills. As a matter of fact, more academics are seeing the benefits of GBL (Qian & Clark, 2016), in which games are specifically designed to enhance learning and the development of a certain skillset. It may also involve adapting a game that is already designed for use in the classroom, such as using open world-based board games to teach the interaction between geopolitical groups, or the use of LEGO® blocks to help improve comprehension of city planning. A good GBL intervention will ensure that the central mechanic of a game is linked to the expected learning outcomes of the module (Arnab et al., 2014).
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- 2023
156. TEGA Toolkit
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Rye, Sara, Abdullahi, Yama, Ennami, Firdaous, Sousa, Carla, Luz, Filipe, Maratou, Vicky, Chaliampalias, Rizos, Kameas, Achilles, Medeišienė, Raimonda Agnė, Ščiukauskė, Indrė, and Karaša, Darius
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TEGA toolkit that aims to support educators in creating inclusive educational games and enhancing game-based learning experiences.
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- 2023
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157. Combinatorial therapies for spinal cord injury repair.
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Sousa, Carla S., Monteiro, Andreia, Salgado, António J., and Silva, Nuno A.
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- 2025
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158. Cartilha Para descentralização da Prep no Municipio de Belém
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Pereira, Camilo Eduardo Almeida, Leal, Tamilis Feitosa, Lima, Vittor Nina de, Sales, Juliana Lavareda, Sousa, Carla Quaresma Durães de, Sousa, Eduarda Barbosa Evangelista de, Silva, Ingred Amanda Brito da, Lima, Gabriele do Nascimento, Gomes, Naellem Filocreão Batista Portilho, Silvia, Samya Pureza da, Silva, André Vilhena, Barra, Edgar ramos, Galvão, José Jorge da Silva, Norte, Raimunda Silvia Gatti, and Souza, Charles Victor Gomes De Souza
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Cartilha Para Descentralização Da Prep no municipio de Belém
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- 2022
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159. Protocolo para Descentralização da Prep no Município de Belém
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Sousa, Carla Quaresma Durães de, Sousa, Eduarda Barbosa Evangelista de, Silva, Ingred Amanda Brito da, Lima, Gabriele do Nascimento, Gomes, Naellem Filocreão Batista Portilho, Silva, Samya Pureza da, Silva, André vilhena da, Barra, Edgar Ramos, Pereira, Camilo Eduardo Almeida, Sales, Juliana Lavareda, Lima, Vittor Nina de, Leal, Tamilis Feitosa Leal, Galvão, José Jorge da Silva, Norte, Raimunda Silvia Gatti, Souza, Charles Victor Gomes de, Barbosa, Juliane Larissa, and Siqueira, Giselli de Oliveira
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Protocolo para descentralização da Prep no município de Belém
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- 2022
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160. Screening of naturalWolbachiainfection in mosquitoes (Diptera: Culicidae) from Cape Verde Islands
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Fernandes da Moura, Aires Januário, primary, Valadas, Vera, additional, Veiga Leal, Silvania Da, additional, Sousa, Carla A., additional, and Pinto, João, additional
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- 2022
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161. Forecasting the numbers of disease vectors with deep learning
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Ceia-Hasse, Ana, primary, Sousa, Carla A., additional, Gouveia, Bruna R., additional, and Capinha, César, additional
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- 2022
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162. CONSULTA DE ENFERMAGEM AMBULATORIAL EM CARDIOLOGIA
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Dos Santos, Ana Carolina Lobo, primary, Costa, Maria Cláudia Medeiros Dantas Rubim, additional, Sousa, Carla Suellen Pires de, additional, Menezes, Lídia Stella Teixeira de, additional, Lima, Jeruza Mara de Oliveira, additional, and Maia, Ivana Cristina Vieira de Lima, additional
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- 2022
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163. Simulated tempering-enhanced umbrella sampling improves convergence of free energy calculations of drug membrane permeation
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Sousa, Carla F., primary, Becker, Robert A., additional, Lehr, Claus-Michael, additional, Kalinina, Olga V., additional, and Hub, Jochen S., additional
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- 2022
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164. Tumour necrosis factor plays a deleterious role in the pathogenesis of chikungunya virus infection
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Moreira, Thaiane Pinto, primary, Sousa, Carla Daiane Ferreira de, additional, Melo Costa, Victor Rodrigues de, additional, Queiroz‐Junior, Celso Martins, additional, Santos, Franciele Martins, additional, Bonilha, Caio Santos, additional, Ésper, Lísia Maria, additional, Nogueira, Mauricio Lacerda, additional, Cunha, Thiago Mattar, additional, Teixeira, Mauro Martins, additional, Costa, Vivian Vasconcelos, additional, and de Souza, Daniele da Glória, additional
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- 2022
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165. Morphological and Molecular Characterization Using Genitalia and CoxI Barcode Sequence Analysis of Afrotropical Mosquitoes with Arbovirus Vector Potential
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Montalvo-Sabino, Eddyson, primary, Abílio, Ana Paula, additional, Guarido, Milehna Mara, additional, Valadas, Vera, additional, Novo, Maria Teresa, additional, Kampango, Ayubo, additional, Sousa, Carla Alexandra, additional, Fafetine, José, additional, Venter, Marietjie, additional, Thompson, Peter N., additional, Braack, Leo, additional, Cornel, Anthony John, additional, Parreira, Ricardo, additional, and de Almeida, António Paulo Gouveia, additional
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- 2022
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166. AN ACCESSIBLE AND INCLUSIVE FUTURE FOR TABLETOP GAMES AND LEARNING: PARADIGMS AND APPROACHES
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Sousa, Carla, primary, Luz, Filipe, additional, Fonseca, Maria Micaela, additional, Neves, Pedro, additional, Lopes, Phil, additional, Maratou, Vicky, additional, Chaliampalias, Rizos, additional, Kameas, Achilles, additional, Abdullahi, Yama, additional, and Rye, Sara, additional
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- 2022
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167. Mapping the Inclusion of Children and Youth With Disabilities in Media Literacy Research
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Sousa, Carla, primary and Costa, Conceição, additional
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- 2022
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168. Modeling the Effect of Hydrophobicity on the Passive Permeation of Solutes across a Bacterial Model Membrane
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Sousa, Carla F., primary, Kamal, Mohamed A.M., additional, Richter, Robert, additional, Elamaldeniya, Kalanika, additional, Hartmann, Rolf W., additional, Empting, Martin, additional, Lehr, Claus-Michael, additional, and Kalinina, Olga V., additional
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- 2022
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169. 2022-RA-1541-ESGO Small cell neuroendocrine tumor of the cervix with multiple cutaneous metastasis: a report of 2 cases
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Bonetti, Heloise Serafin, primary, de Sousa, Carla Baltazar, additional, Sartorelli, Vivian, additional, Baptista da Silva, Renan Tapigliani, additional, de Almeida, Thais Gomes, additional, de Souza Pinto, Gabriel Lowndes, additional, de Farias e Silva, Andre Lopes, additional, Guilherme, Andriolli, additional, Persiano, Marcella Bastos, additional, D’Elia, Giovana Mortari, additional, Garcia, Rafaela Debastiani, additional, Vieira Sales, Maria Jannine, additional, Cardoso, Amanda Horie, additional, and Cintra, Georgia Fontes, additional
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- 2022
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170. Inclusive AR-games for Education of Deaf Children: Challenges and Opportunities
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Westin, Thomas, primary, Neves, José, additional, Mozelius, Peter, additional, Sousa, Carla, additional, and Mantovan, Lara, additional
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- 2022
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171. The current and future distribution of the yellow fever mosquito (Aedes aegypti) on Madeira Island
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Santos, José Maurício, primary, Capinha, César, additional, Rocha, Jorge, additional, and Sousa, Carla Alexandra, additional
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- 2022
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172. Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure
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Trindade, Fábio, primary, Ferreira, Ana F., additional, Saraiva, Francisca, additional, Martins, Diana, additional, Mendes, Vera M., additional, Sousa, Carla, additional, Gavina, Cristina, additional, Leite-Moreira, Adelino, additional, Manadas, Bruno, additional, Falcão-Pires, Inês, additional, and Vitorino, Rui, additional
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- 2022
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173. Epidemic Marketplace: An Information Management System for Epidemiological Data
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Lopes, Luis F., Silva, Fabrício A. B., Couto, Francisco, Zamite, João, Ferreira, Hugo, Sousa, Carla, Silva, Mário J., Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Khuri, Sami, editor, Lhotská, Lenka, editor, and Pisanti, Nadia, editor
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- 2010
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174. Global longitudinal strain as a potential prognostic marker in patients with chronic heart failure and systolic dysfunction
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Rangel, Inês, Gonçalves, Alexandra, de Sousa, Carla, Almeida, Pedro Bernardo, Rodrigues, João, Macedo, Filipe, Silva Cardoso, José, and Maciel, M<ce:sup loc='post">a</ce:sup> Júlia
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- 2014
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175. The usefulness of the head-up tilt test in patients with suspected epilepsy
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Rangel, Inês, Freitas, João, Correia, Ana Sofia, Sousa, Alexandra, Lebreiro, Ana, de Sousa, Carla, Maciel, Maria Júlia, and Azevedo, Elsa
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- 2014
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176. Telemonitoring in heart failure: A state-of-the-art review
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Sousa, Carla, Leite, Sérgio, Lagido, Ricardo, Ferreira, Liliana, Silva-Cardoso, José, and Maciel, Maria Júlia
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- 2014
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177. Functional Feeds to Tackle Meagre (Argyrosomus regius) Stress: Physiological Responses under Acute Stressful Handling Conditions
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Universidade do Porto (Portugal), European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Monteiro, Marta [0000-0002-9968-8575], Sousa, Carla [0000-0003-2285-6507], Fontinha, Filipa [0000-0003-3906-1735], Guerreiro, Inês [0000-0002-3072-8364], Pousão-Ferreira, P. [0000-0001-6746-764X], Matos, Elisabete [0000-0001-8128-8963], Díaz-Rosales, Patricia [0000-0001-6965-3702], Oliva-Teles, Aires [0000-0002-5730-836X], Enes, Paula [0000-0002-6248-0500], Monteiro, Marta, Sousa, Carla, Coutinho, Filipe, Castro, Carolina, Fontinha, Filipa, Guerreiro, Inês, Pousão-Ferreira, P., Matos, Elisabete, Díaz-Rosales, Patricia, Oliva-Teles, Aires, Enes, Paula, Couto, Ana, Universidade do Porto (Portugal), European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Monteiro, Marta [0000-0002-9968-8575], Sousa, Carla [0000-0003-2285-6507], Fontinha, Filipa [0000-0003-3906-1735], Guerreiro, Inês [0000-0002-3072-8364], Pousão-Ferreira, P. [0000-0001-6746-764X], Matos, Elisabete [0000-0001-8128-8963], Díaz-Rosales, Patricia [0000-0001-6965-3702], Oliva-Teles, Aires [0000-0002-5730-836X], Enes, Paula [0000-0002-6248-0500], Monteiro, Marta, Sousa, Carla, Coutinho, Filipe, Castro, Carolina, Fontinha, Filipa, Guerreiro, Inês, Pousão-Ferreira, P., Matos, Elisabete, Díaz-Rosales, Patricia, Oliva-Teles, Aires, Enes, Paula, and Couto, Ana
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Marine algae are recognised sources of bioactive compounds that have attracted great interest as nutritional supplements for aquaculture fish. Intensive rearing conditions often expose fish to husbandry-related stressors, rendering fish more susceptible to disease and reducing production yields. The present work evaluated the potential of two marine algae extracts (Fucus vesiculosus and Nannochloropsis gaditana) as nutritional supplements to mitigate stress effects in meagre (Argyrosomus regius) exposed to an acute handling stress (AS). A plant-based diet was used as a control, and three other diets were prepared, which were similar to the control diet but supplemented with 1% of each algal extract or a combination of the two extracts (0.5% each). The effects of supplemented diets on stress biomarkers, antioxidant enzyme activities, and immune response were analysed in fish exposed to AS after 4 weeks of feeding. Supplemented diets did not affect growth performance but the inclusion of F. vesiculosus promoted higher feed efficiency, as compared to the control group. Dietary algal extracts supplementation reduced plasma glucose levels, increased white blood cell counts, and reduced the expression of pro-inflammatory genes when compared with the control. N. gaditana supplementation led to a reduction in hepatic antioxidant enzyme activity and glutathione levels, while F. vesiculosus supplementation increased muscle glutathione reductase activity and reduced lipid peroxidation. These findings support the potential of algal extracts as nutraceuticals in aquafeeds to enhance the ability of fish to cope with husbandry-related stressful conditions and ultimately improve fish health and welfare.
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- 2021
178. Consumo consciente: boas práticas da a3p – IFES – campus Guarapari
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de Quadros, M., primary, R. da Silva, Caio, additional, R de Sousa, Carla, additional, H. Carneiro, Cristina, additional, de B. F. Gomes, Eduarda, additional, C. O. Martins, Fabiola, additional, S. Carvalho, Helliene, additional, G. Hatab, Layla, additional, T. Machado, Marcelo, additional, A. A. da S. Souza, Maria, additional, S. P. Meriguete, Morgana, additional, N. Cruz, Ronaldo, additional, and de P. B. Carvalho, Virginia, additional
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- 2017
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179. Competitive Intelligence Competitive Intelligence
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Fernandes da Anunciação, Pedro, primary, Zambon, António Carlos, additional, Andrade, Fernanda de Jesus, additional, and Sanches de Sousa, Carla S., additional
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- 2017
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180. Potential of Aedes aegypti populations in Madeira Island to transmit dengue and chikungunya viruses
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Seixas, Gonçalo, Jupille, Henri, Yen, Pei-Shi, Viveiros, Bela, Failloux, Anna-Bella, and Sousa, Carla Alexandra
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- 2018
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181. USE OF COMBINED HORMONAL CONTRACEPTIVES OCCURRENCE OF METABOLIC SYNDROME: A REVIEW
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de Sousa, Carla
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fungi ,food and beverages - Abstract
The goal of this revision is to clarify if CHCs use can be related to metabolic alterations which may have influence in metabolic syndrome occurrence.
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- 2022
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182. Atención multiprofesional a pacientes com carcinoma hepatocelular: relato de experiencia
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Fernandes, Pedro Henrique dos Santos, Almeida, Ysis Nayhara Raiol de, Moreira, Ana Jhennyfer da Silva, Nascimento , Monique Teresa Amoras, Solano , Ariel Christine dos Anjos, Lopes , Suelen Tainá Lima, Sousa, Carla Quaresma Durães de, Nascimento , Carla Simone Andrade do, Silva, José Henrique Santos, and Silva, Edileuda da
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Interprofessional education ,Medical oncology ,Oncologia ,Educação interprofissional ,Carcinoma hepatocellular ,Problem-based learning ,Éducation interprofessionnelle ,Carcinoma hepatocelular ,Aprendizagem baseada em problemas ,Aprendizaje basado en problemas ,Oncología médica - Abstract
Liver cancer is called the fifth most incident type of neoplasm and fourth with prominence in the causes of death worldwide, within this branch the hepatocellular carcinoma stands out, being mostly asymptomatic. From this, active methodologies are extremely relevant during learning in health courses. Therefore, the objective of this work was to approach an experience report of the performance of a multidisciplinary team of undergraduates in the health area in the resolution of a fictitious clinical case about a patient with hepatocellular carcinoma using an active methodology. The experience of the activities took place in September 2021 during a theoretical class in which the resolution of a contextualized clinical case was proposed for the formulation of a multidisciplinary therapeutic plan. In view of this, it was possible to design a unique multiprofessional therapeutic plan, making the appropriate choice of treatment according to the clinical case in question, highlighting the contribution of active methodologies in the training of undergraduates in the health area. In short, such methods are of great value in amplifying knowledge about the subject in question, thus appropriating the teaching-learning axis within oncology, in addition to encouraging critical and reflective thinking in undergraduates. El cáncer de hígado se denomina el quinto tipo de neoplasia más incidente y el cuarto con protagonismo en las causas de muerte a nivel mundial, dentro de esta rama se destaca el carcinoma hepatocelular, siendo en su mayoría asintomático. A partir de esto, las metodologías activas son extremadamente relevantes durante el aprendizaje en cursos de salud. Por ello, el objetivo de este trabajo fue abordar un relato de experiencia de la actuación de un equipo multidisciplinario de estudiantes del área de la salud en la resolución de un caso clínico ficticio sobre un paciente con hepatocarcinoma utilizando una metodología activa. La experiencia de las actividades se llevó a cabo en septiembre de 2021 durante una clase teórica en la que se planteó la resolución de un caso clínico contextualizado para la formulación de un plan terapéutico multidisciplinario. Ante ello, se logró diseñar un plan terapéutico multiprofesional único, efectuando la elección del tratamiento adecuado según el caso clínico en cuestión, destacando el aporte de las metodologías activas en la formación de los estudiantes de grado en el área de la salud. En definitiva, tales métodos son de gran valor para ampliar el conocimiento sobre el tema en cuestión, apropiándose así del eje de enseñanza-aprendizaje dentro de la oncología, además de fomentar el pensamiento crítico y reflexivo en los estudiantes de pregrado. O câncer de fígado é denominado o quinto tipo de neoplasia mais incidente e quarto com destaque nas causas de óbito em todo o mundo, dentro dessa ramificação destaca-se o carcinoma hepatocelular, sendo em sua maioria assintomático. A partir disso, as metodologias ativas são de extrema relevância durante a aprendizagem nos cursos da área da saúde. Sendo assim, o trabalho objetivou-se abordar um relato de experiência da atuação de uma equipe multiprofissional de graduandos da área da saúde na resolução de um caso clínico fictício sobre um paciente com carcinoma hepatocelular utilizando-se de metodologia ativa. A vivência das atividades ocorreu no mês de setembro de 2021 durante uma aula teórica na qual foi proposta a resolução de caso clínico contextualizado para formulação de um plano terapêutico multidisciplinar. Diante disso, foi possível traçar um plano terapêutico singular multiprofissional, realizando a escolha adequada do tratamento de acordo com o caso clínico em questão, destacando a contribuição das metodologias ativas na formação dos graduandos da área da saúde. Em suma, tais métodos são de grande valia na amplificação dos conhecimentos acerca do assunto em questão, dessa forma, se apropriando do eixo ensino-aprendizagem dentro da oncologia, além de incentivar o pensamento crítico e reflexivo dos graduandos.
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- 2022
183. Annexin A1-FPR2/ALX Signaling Axis Regulates Acute Inflammation during Chikungunya Virus Infection
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de Araújo, Simone, primary, de Melo Costa, Victor R., additional, Santos, Franciele M., additional, de Sousa, Carla D. Ferreira, additional, Moreira, Thaiane P., additional, Gonçalves, Matheus R., additional, Félix, Franciel B., additional, Queiroz-Junior, Celso M., additional, Campolina-Silva, Gabriel H., additional, Nogueira, Maurício Lacerda, additional, Sugimoto, Michelle A., additional, Bonilha, Caio S., additional, Perretti, Mauro, additional, Souza, Danielle G., additional, Costa, Vivian V., additional, and Teixeira, Mauro M., additional
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- 2022
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184. Igestsaúde – a autogestão dos sintomas na pessoa em tratamento de quimioterapia: uma revisão integrativa da literatura
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Sousa, Carla, primary, Magalhães, Bruno, additional, Rafael, Marisa, additional, Fernandes, Carla, additional, and Santos, Célia, additional
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- 2022
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185. Atenção multiprofissional a paciente portador de carcinoma hepatocelular: um relato de experiência
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Fernandes, Pedro Henrique dos Santos, primary, Almeida, Ysis Nayhara Raiol de, additional, Moreira, Ana Jhennyfer da Silva, additional, Nascimento, Monique Teresa Amoras, additional, Solano, Ariel Christine dos Anjos, additional, Lopes, Suelen Tainá Lima, additional, Sousa, Carla Quaresma Durães de, additional, Nascimento, Carla Simone Andrade do, additional, Silva, José Henrique Santos, additional, and Silva, Edileuda da, additional
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- 2022
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186. Saúde discente em uma universidade pública: um estudo no nordeste brasileiro
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Araújo Lopes Vieira, Camilla, primary, Pinheiro, Francisco Pablo Huascar Aragão, additional, Ribeiro de Sousa, Carla, additional, Lima, Carlos Magno Sérgio, additional, Sá Cunha, Esthela, additional, Teles Aguiar, Hellyne Maria, additional, and Ponte Alcântara, Vírnia, additional
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- 2022
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187. Diversity and Big Trees Patterns in the Brazilian Amazon
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de Lima, Robson Borges, primary, Görgens, Eric Bastos, additional, Batista, Anderson Pedro Bernardina, additional, da Silva, Diego Armando Silva, additional, de Oliveira, Cinthia Pereira, additional, and de Sousa, Carla Samara Campelo, additional
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- 2022
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188. Distinct fatty acid profile of ten brown macroalgae
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Silva, Graça, Pereira, Renato B., Valentão, Patrícia, Andrade, Paula B., and Sousa, Carla
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- 2013
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189. Investigating Inclusivity in Game-Based Learning: Current Practices and Multistakeholder Perspectives.
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Rye, Sara and Sousa, Carla
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GAMIFICATION ,MACHINE learning ,DIGITAL technology ,ARTIFICIAL intelligence ,EDUCATIONAL games - Abstract
This study aims to examine how inclusivity measures are understood and applied in game-based learning (GBL). It considers the perspectives of various stakeholders, such as educators, game designers, and students. The focus is on creating accessible and engaging games that meet the diverse needs and characteristics of players. The methodology adopted a combination of primary and secondary data sources to pursue these aims. The primary data collection involved focus groups with educators, game designers, and students. The study employed a participatory design approach, involving multiple stakeholders in the exploration of inclusivity measures. The data collected from the focus groups, along with findings from the literature review, helped in formulating a set of inclusivity metrics for educators to create educational games that cater to diverse student needs. The obtained results emphasize the limited state of analogue GBL accessibility in scholarly and professional literature, while emphasizing the existing frameworks to be adopted by educators, designers, and publishers. Stakeholder discussions revealed themes related to inclusivity measures, including motor, sensory, and cognitive needs of players. Game designers can enhance accessibility by considering these requirements and incorporating alternative communication channels, accessible cues, adaptable gameplay options, and diversified knowledge-based requirements. In addition to inclusivity, addressing instances of exclusion, managing teams effectively, promoting inclusive communication, and incorporating gameplay limitations, educational components, diverse perspectives, and real-world applicability are discussed as important in education game design, to this extent. [ABSTRACT FROM AUTHOR]
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- 2023
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190. The Novelty of Collaboration: High School Students Learning and Enjoyment Perceptions When Playing Cooperative Modern Board Games.
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Sousa, Micael, Sousa, Carla, and Luz, Filipe
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HIGH school students ,ARTIFICIAL intelligence ,DIGITAL technology ,SCHOOL children ,EDUCATIONAL games ,TECHNOLOGICAL innovations - Abstract
Modern board games are changing the way people play analogue face-to-face multiplayer games. One of the novelties of this trend is the growing prevalence of games that offer collaborative gameplay. These cooperative games have been instrumental in shifting the demographic of players, attracting individuals who prefer engaging in constructive interactions rather than destructive confrontations. Likewise, playing cooperative games demand specific players' skills which can be associated with the concept of soft skills. Training these skills through modern cooperative board games, whether by modifying existing games or developing new ones as serious games or using gamification approaches, holds promising potential. To test the perception of students playing cooperative modern board games for the first time, we organized a one-day play session in a school environment, specifically in a classroom with the support of the students' teachers. Over a period of six hours (three in the morning and three in the afternoon), a total of 25 high school students from the vocational multimedia area, comprising students of all genders, participated in the session. The students engaged in gameplay with a curated selection of four cooperative modern board games. The results indicate that the students enjoyed the game sessions and recognized the significance of collaboration, as well as the associated skills including communication, empathy, organization, focus, and complementary interactions. The experiment reinforces the suggestion that the used modern board games could be introduced during classes as engaging activities fit for learning and training. [ABSTRACT FROM AUTHOR]
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- 2023
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191. Mathematics and Sign Language Learning With a Tangible Game: An Inclusive Approach for DHH and Hearing Children.
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Casimiro, Cátia, Carlos Neves, José, and Sousa, Carla
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SIGN language ,MATHEMATICS education ,ARTIFICIAL intelligence ,DIGITAL technology ,SCHOOL children ,EDUCATIONAL games ,TECHNOLOGICAL innovations - Abstract
The purpose of this study was to investigate the potential of a tangible game, Inclusive Glossary of Mathematical Terms (GIM), to support the teaching of math and Portuguese sign language (Língua Gestual Portuguesa; LGP) to preschool and first-grade children, with an emphasis on inclusive education, knowledge acquisition, and engagement. GIM is composed of physical cards that are complemented by digital videos that run on an interface designed for this game. The game consists of two sets of cards and respective animations. As an inclusive math glossary, it also includes videos where concepts, characters, and objects are identified through images, words, and sign language. During the development process of GIM, it was possible to involve a multidisciplinary team, of which a Deaf sign language teacher and a sign language interpreter were part to ensure the clarity of the sign language and refine the written sentences accompanying the glossary. In order to validate its contents, a focus group of preschool and primary teachers specialized in the teaching of Deaf and Hard of Hearing (DHH) children was formed. A total of 120 children, aged between three and eight years old (M = 5.61; SD = 0.78), and seven LGP and special education teachers from three Portuguese schools participated in the study. The children used GIM in their classrooms, with the assistance of the participating teachers, who were subsequently interviewed to assess their engagement, interest, and learning outcomes. The interviews were analyzed using content analysis procedures, including descriptive statistics and Pearson correlations. The results indicated that GIM has the potential to effectively support the teaching of mathematics and LGP to preschool and first-grade children, especially in inclusive education contexts. The tangible nature of the game facilitated children's understanding of mathematical concepts and LGP, leading to improved involvement in the teaching-learning process. Moreover, the game promoted awareness of diversity and the principles of inclusive education, making it a valuable tool for promoting social inclusion and understanding. However, the study has reduced sample and context-specific limitations, suggesting a need for further research in this area. [ABSTRACT FROM AUTHOR]
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- 2023
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192. Barriers and Hindrances to the Effective use of Games in Education: Systematic Literature Review and Intervention Strategies.
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Sousa, Carla, Neves, Pedro Pinto, and Luz, Filipe
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ARTIFICIAL intelligence ,DIGITAL technology ,SCHOOL children ,EDUCATIONAL games ,TECHNOLOGICAL innovations - Abstract
Games play a central role in human culture, as well as in interaction, interpersonal relationships, and overall development. Based on these premises, game-based learning (GBL) has been increasingly studied as a form of pedagogical innovation in formal and non-formal education. However, the implementation of these types of interventions in real educational settings has faced several barriers and constraints that reduce their possible impact. In this context, the present study aims to fulfil two aims: (a) mapping the main obstacles experienced in the implementation of GBL approaches in educational contexts; and (b) proposing an intervention diagram that matches each type of barrier with potential strategies to address it. Towards these aims, a Systematic Literature Review (SLR) was conducted, following the PRISMA methodology and guidelines. This included screening and eligibility processes based on inclusion criteria, which were defined considering the research's aims. Non-peer-reviewed research and studies aimed at other pedagogical approaches, such as gamification, were excluded to obtain a final sample of 11 studies and 1952 individuals, with an average of 195.20 participants per study (SD = 266.14). Through the analysis of the studies, a model of four types of barriers emerged: (1) attitudinal and behavioural barriers; (2) school policy barriers; (3) technological and material barriers; and (4) game literacy barriers. Through the critical evaluation of the barriers emerging from the analyzed empirical studies, we propose some potential methodological strategies that might support practitioners in overcoming them. This includes: (a) the implementation of a co-creation and participatory-driven methodology; (b) the appropriate selection of analogue, digital, or hybrid games; (c) the inclusion of the different educational stakeholders in the process; and (d) the promotion of GBL training within the projects. [ABSTRACT FROM AUTHOR]
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- 2023
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193. The Dark Side of Fun: Understanding Dark Patterns and Literacy Needs in Early Childhood Mobile Gaming.
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Sousa, Carla and Oliveira, Ana
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MOBILE games ,ARTIFICIAL intelligence ,DIGITAL technology ,SCHOOL children ,EDUCATIONAL games - Abstract
Play has always been recognized as an essential aspect of human development, particularly during early childhood, as it contributes to learning, the formation of meanings, and experiencing the world. In today's digitalized society, early childhood education has increasingly integrated digital media into its practices, both in schools and at the family level. Mobile digital games (MDG) have received significant attention due to their impact on children's interactions, play, and learning. However, as young children engage more with MDGs, concerns about problem gaming have arisen, referring to conflicts and issues that emerge from game playing within everyday sociocultural contexts. Scholars such as Zagal et al. (2013) have identified certain game design patterns as "dark", which can be considered unethical as they manipulate players against their best interests. Given the prevalence of mobile gaming in early childhood, studying these dark patterns becomes even more crucial. This study aims to investigate the presence of dark patterns in MDG for young children (0-5 years old), through qualitative analysis. The five most popular free games for this age range on App Store (February 2023) were analysed, particularly focusing on the presence of temporal, monetary, social, and/or psychological dark patterns. The analysis uncovers the presence of temporal, monetary, and psychological dark patterns, including aesthetic manipulations, paywalls, and periodic rewards resembling gambling elements. The games also employ advertising strategies and engagement tactics that challenge young children's navigation. Parental control mechanisms offer limited safeguards, requiring continuous monitoring and parental involvement in play dynamics. The study highlights the importance of adult media and digital literacy in supporting children's online play effectively, while also emphasising the responsibility of game designers and developers to create healthier and less risky game experiences. [ABSTRACT FROM AUTHOR]
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- 2023
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194. Mapping the Inclusion of Children and Youth With Disabilities in Media Literacy Research
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Fundação para a Ciência e Tecnologia (FCT), Sousa, Carla, Costa, Conceição, Fundação para a Ciência e Tecnologia (FCT), Sousa, Carla, and Costa, Conceição
- Abstract
The way we communicate and make meaning in a complex socio-technical infrastructure demands multiple literacies. Media-literate citizens must be able to create, evaluate and effectively use information, media, and technology. The pandemic context demanded increased online learning and work, highlighting the importance of media literacy in citizens’ lives. Although in recent years, crucial reforms have happened in education for children with disabilities, media education for them is residual and framed on medical concepts neglecting how disability is socially constructed. Aiming to map recent research (2015–2021) in the field of media literacy and children with disabilities, a systematic literature review was conducted. The number of articles obtained from a search for “media literacy and children” in the scientific databases (N = 1,175) supports the relevance of media literacy in research. Filtering these data for “children with disabilities” reveals an inexpressive sample, with 12 articles included in the study after the eligibility phase. The overall results indicate that this population is significantly underrepresented in media literacy research, explained by a low prevalence of studies with disabled children as an audience. Moreover, research designs have shown a greater focus on conceptual approaches, highlighting a deficit of fieldwork and tangible interventions. Strong ableist media discourses emerged as a barrier to the promotion of media literacy in this population, with a clear mismatch between media representations and the current disability paradigms, besides all the positive aspects of the actions registered in the sample.
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- 2022
195. Inclusive AR-games for Education of Deaf Children : Challenges and Opportunities
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Westin, Thomas, Neves, José Carlos, Mozelius, Peter, Sousa, Carla, Mantovan, Lara, Westin, Thomas, Neves, José Carlos, Mozelius, Peter, Sousa, Carla, and Mantovan, Lara
- Abstract
Game-based learning has had a rapid development in the 21st century, attracting an increasing audience. However, inclusion of all is still not a reality in society, with accessibility for deaf and hard of hearing children as a remaining challenge. To be excluded from learning due to communication barriers can have severe consequences for further studies and work. Based on previous research Augmented Reality (AR) games can be joyful learning tools that include activities with different sign languages, but AR based learning games for deaf and hard of hearing lack research. This paper aims to present opportunities and challenges of designing inclusive AR games for education of deaf children. Methods involved conducting a scoping review of previous studies about AR for deaf people. Experts were involved as co-authors for in-depth understanding of sign languages and challenges for deaf people. A set of AR input and output techniques were analysed for appropriateness, and various AR based game mechanics were compared. Results indicate that inclusive AR gameplay for deaf people could be built on AR based image and object tracking, complemented with sign recognition. These technologies provide input from the user and the real-world environment typically via the camera to the app. Scene tracking and GPS can be used for location-based game mechanics. Output to the user can be done via local signed videos ideally, but also with images and animations. Moreover, a civic intelligence approach can be applied to overcome many of the challenges that have been identified in five dimensions for inclusion of deaf people i.e., cultural, educational, psycho-social, semantic, and multimodal. The input from trusted, educated signers and teachers can enable the connection between real world objects and signed videos to provide explanations of concepts. The conclusion is that the development of an inclusive, multi-language AR game for deaf people needs to be carried out as an international c
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- 2022
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196. Applying genomic approaches to delineate conservation strategies using the freshwater mussel Margaritifera margaritifera in the Iberian Peninsula as a model
- Author
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Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Perea, Silvia, Mendes, Sofía L., Sousa-Santos, Carla, Ondina Navarret, María Paz, Amaro González, Rafaela María, Castro, Jaime, San Miguel, Eduardo, García, María, García-Roves, Pedro, Fernández, Diego, Araujo, Rafael, Sousa, Carla, Velasquez, Víctor, Lima, Cristina, Sousa, Víctor, Reis, Joaquim, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Perea, Silvia, Mendes, Sofía L., Sousa-Santos, Carla, Ondina Navarret, María Paz, Amaro González, Rafaela María, Castro, Jaime, San Miguel, Eduardo, García, María, García-Roves, Pedro, Fernández, Diego, Araujo, Rafael, Sousa, Carla, Velasquez, Víctor, Lima, Cristina, Sousa, Víctor, and Reis, Joaquim
- Abstract
Effective conservation actions to counteract the current decline of populations and species require a deep knowledge on their genetic structure. We used Single Nucleotide Polymorphisms (SNPs) to infer the population structure of the highly threatened freshwater pearl mussel Margaritifera margaritifera in the Iberian Peninsula. A total of 130 individuals were collected from 26 locations belonging to 16 basins. We obtained 31,692 SNPs through Genotyping by Sequencing (GBS) and used this dataset to infer population structure. Genetic diversity given as observed heterozygosity was low. Pairwise FST comparisons revealed low levels of genetic differentiation among geographically close populations. Up to 3 major genetic lineages were determined: Atlantic, Cantabrian and Douro. This structure suggests a close co‑evolutionary process with brown trout (Salmo trutta), the primordial fish host of this mussel in the studied area. Some sub‑basins showed some genetic structuring, whereas in others no intrapopulation differentiation was found. Our results confirm that genetic conservation units do not match individual basins, and that knowledge about the genetic structure is necessary before planning recovery plans that may involve relocation or restocking. The same reasoning should be applied to strictly freshwater species that are sessile or have restricted dispersal abilities and are currently imperiled worldwide., Las acciones de conservación efectivas que conduzcan a contrarrestar el declive actual de poblaciones y especies requieren un conocimiento profundo de su estructura genética. En este trabajo se han utilizado polimorfismos de nucleótido único (SNPs) para inferir la estructura poblacional en la Peninsula Ibérica de la especie en peligro de extinción Margaritifera margaritifera. Se recogieron 130 individuos en 26 localidades pertenecientes a 16 cuencas. Obtuvimos 31.692 SNPs mediante Genotipado por Secuenciación (GBS) y se utilizó este conjunto de datos para inferir la estructura poblacional. La diversidad genética expresada como heterocigosidad observada fue baja y las comparaciones FST por pares revelaron bajos niveles de diferenciación genética entre poblaciones geográficamente próximas. Se determinaron hasta 3 linajes genéticos principales: Atlántico, Cantábrico y Duero. Esta estructura sugiere un estrecho proceso coevolutivo con la trucha común (Salmo trutta), el pez hospedador primordial de este mejillón en la zona estudiada. Algunas subcuencas mostraron cierta estructuración genética, mientras que en otras no se encontró diferenciación intrapoblacional. Los resultados confirman que las unidades de conservación genética no coinciden con las cuencas individuales, y que es necesario conocer la estructura genética antes de planificar planes de recuperación que puedan implicar reubicaciones o repoblaciones. El mismo razonamiento debería aplicarse a las especies estrictamente de agua dulce que son sésiles o tienen una capacidad de dispersión restringida y se encuentran actualmente en peligro en todo el mundo.
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- 2022
197. Soluble triggering receptor expressed on myeloid cells-1 is a marker of organ injuries in cardiogenic shock:results from the CardShock Study
- Author
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Kimmoun, Antoine, Duarte, Kevin, Harjola, Veli Pekka, Tarvasmäki, Tuukka, Levy, Bruno, Mebazaa, Alexandre, Gibot, Sebastien, Koniari, Katerina, Voumvourakis, Astrinos, Karavidas, Apostolos, Parissis, John, Sans-Rosello, Jordi, Vila, Montserrat, Duran-Cambra, Albert, Sionis, Alessandro, Parenica, Jiri, Stipal, Roman, Ludka, Ondrej, Palsuva, Marie, Ganovska, Eva, Kubena, Petr, Spinar, Jindrich, Lindholm, Matias G., Hassager, Christian, Køber, Lars, Bäcklund, Tom, Lassus, Johan, Jurkko, Raija, Tolppanen, Heli, Nieminen, Markku S., Järvinen, Kristiina, Nieminen, Tuomo, Pulkki, Kari, Soininen, Leena, Sund, Reijo, Tierala, Ilkka, Tolonen, Jukka, Varpula, Marjut, Korva, Tuomas, Pietilä, Mervi, Pitkälä, Anne, Marino, Rossella, Di Somma, Salvatore, Metra, Marco, Bulgari, Michela, Lazzarini, Valentina, Carubelli, Valentina, Sousa, Alexandra, Silva-Cardoso, Jose, Sousa, Carla, Kimmoun, Antoine, Duarte, Kevin, Harjola, Veli Pekka, Tarvasmäki, Tuukka, Levy, Bruno, Mebazaa, Alexandre, Gibot, Sebastien, Koniari, Katerina, Voumvourakis, Astrinos, Karavidas, Apostolos, Parissis, John, Sans-Rosello, Jordi, Vila, Montserrat, Duran-Cambra, Albert, Sionis, Alessandro, Parenica, Jiri, Stipal, Roman, Ludka, Ondrej, Palsuva, Marie, Ganovska, Eva, Kubena, Petr, Spinar, Jindrich, Lindholm, Matias G., Hassager, Christian, Køber, Lars, Bäcklund, Tom, Lassus, Johan, Jurkko, Raija, Tolppanen, Heli, Nieminen, Markku S., Järvinen, Kristiina, Nieminen, Tuomo, Pulkki, Kari, Soininen, Leena, Sund, Reijo, Tierala, Ilkka, Tolonen, Jukka, Varpula, Marjut, Korva, Tuomas, Pietilä, Mervi, Pitkälä, Anne, Marino, Rossella, Di Somma, Salvatore, Metra, Marco, Bulgari, Michela, Lazzarini, Valentina, Carubelli, Valentina, Sousa, Alexandra, Silva-Cardoso, Jose, and Sousa, Carla
- Abstract
Aims: Optimal outcome after cardiogenic shock (CS) depends on a coordinated healing response in which both debris removal and extracellular matrix tissue repair play a crucial role. Excessive inflammation can perpetuate a vicious circle, positioning leucocytes as central protagonists and potential therapeutic targets. High levels of circulating Triggering Receptor Expressed on Myeloid cells-1 (TREM-1), were associated with death in acute myocardial infarction confirming excessive inflammation as determinant of bad outcome. The present study aims to describe the association of soluble TREM-1 with 90-day mortality and with various organ injuries in patients with CS. Methods and results: This is a post-hoc study of CardShock, a prospective, multicenter study assessing the clinical presentation and management in patients with CS. At the time of this study, 87 patients had available plasma samples at either baseline, and/or 48 h and/or 96–120 h for soluble TREM-1 (sTREM-1) measurements. Plasma concentration of sTREM-1 was higher in 90-day non-survivors than survivors at baseline [median: 1392 IQR: (724–2128) vs. 621 (525–1233) pg/mL, p = 0.008), 48 h (p = 0.019) and 96–120 h (p = 0.029). The highest tertile of sTREM-1 at baseline (threshold: 1347 pg/mL) was associated with 90-day mortality with an unadjusted HR 3.08 CI 95% (1.48–6.42). sTREM-1 at baseline was not associated to hemodynamic parameters (heart rate, blood pressure, use of vasopressors or inotropes) but rather with organ injury markers: renal (estimated glomerular filtration rate, p = 0.0002), endothelial (bio-adrenomedullin, p = 0.018), myocardial (Suppression of Tumourigenicity 2, p = 0.002) or hepatic (bilirubin, p = 0.008). Conclusion: In CS patients TREM-1 pathway is highly activated and gives an early prediction of vital organ injuries and outcome. Graphic abstract: [Figure not available: see fulltext.].
- Published
- 2022
198. Size of sampling units aproppriate for management level forest inventory in Eastern Amazon, Brazil.
- Author
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Campelo de Sousa, Carla Samara, Aleixo da Silva, José Antônio, Caraciolo Ferreira, Rinaldo Luiz, Silva da Silva, Diego Armando, Ferreira da Silva, Evandro, and Borges de Lima, Robson
- Subjects
- *
FOREST management , *FOREST surveys , *SAMPLE size (Statistics) , *TIME measurements , *FOREST dynamics , *BASAL area (Forestry) - Abstract
This study aimed to indicate the ideal sample size for continuous forest inventories in the tropical forest in Amapá. Data were obtained from a monitoring inventory started in 2010 and completed in 2016, in five permanent sampling units of 100m x 100m with an inclusion level of DBH = 10cm. Randomly allocated sample subunits with five different dimensions ranging from 400 m2 to 1600 m2 were simulated within these units. The measurement times of each sampling unit, from the first to the last three, were timed. Dimensions were analyzed for their accuracy and relative efficiency. Given the above and depending on the purpose of the inventory, the standard sampling unit of 1 ha (100 m x 100 m) is recommended for the inventory variables. The 20 m x 20 m (400 m2) square sampling unit was the most suitable for monitoring growth variables (annual increment in diameter, basal area, and volume), indicating that these sampling units for continuous inventories can become an essential alternative for use in monitoring inventory in the region. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
199. Tumour necrosis factor plays a deleterious role in the pathogenesis of chikungunya virus infection.
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Moreira, Thaiane Pinto, Sousa, Carla Daiane Ferreira de, Melo Costa, Victor Rodrigues de, Queiroz‐Junior, Celso Martins, Santos, Franciele Martins, Bonilha, Caio Santos, Ésper, Lísia Maria, Nogueira, Mauricio Lacerda, Cunha, Thiago Mattar, Teixeira, Mauro Martins, Costa, Vivian Vasconcelos, and de Souza, Daniele da Glória
- Subjects
- *
CHIKUNGUNYA virus , *VIRUS diseases , *TUMOR necrosis factor receptors , *ANTIVIRAL agents , *INFLAMMATORY mediators , *ALPHAVIRUSES , *EXPERIMENTAL arthritis - Abstract
Arthralgia is a hallmark of chikungunya virus (CHIKV) infection and can be very debilitating and associated with a robust local inflammatory response. Many pathophysiological aspects associated with the disease remain to be elucidated. Here, we describe a novel model of CHIKV infection in immunocompetent mice and evaluate the role of tumour necrosis factor in the pathogenesis of the disease. C57BL/6 wild type (WT) or TNF receptor 1 deficient (TNFR1−/−) mice were inoculated with 1 × 106 PFU of CHIKV in the paw. Alternatively, etanercept was used to inhibit TNF in infected WT mice. Hypernociception, inflammatory and virological analysis were performed. Inoculation of CHIKV into WT mice induced persistent hypernociception. There was significant viral replication in target organs and local production of inflammatory mediators in early time‐points after infection. CHIKV infection was associated with specific humoral IgM and IgG responses. In TNFR1−/− mice, there was a decrease in the hypernociception threshold, which was associated with a milder local inflammatory response in the paw but delayed viral clearance. Local or systemic treatment with etanercept reduced CHIKV‐induced hypernociception. This is the first study to describe hypernociception, a clinical correlation of arthralgia, in immunocompetent mice infected with CHIKV. It also demonstrates the dual role of TNF in contributing to viral clearance but driving tissue damage and hypernociception. Inhibition of TNF may have therapeutic benefits but its role in viral clearance suggests that viral levels must be monitored in CHIKV‐infected patients and that TNF inhibitors should ideally be used in combination with anti‐viral drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
200. Phytochemical investigations and biological potential screening with cellular and non-cellular models of globe amaranth (Gomphrena globosaL.) inflorescences
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Silva, Luís R., Valentão, Patrícia, Faria, Joana, Ferreres, Federico, Sousa, Carla, Gil-Izquierdo, Angel, Pinho, Brígida R., and Andrade, Paula B.
- Published
- 2012
- Full Text
- View/download PDF
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