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The potential for widespread sink-limited plant growth has received increasing attention in the literature in the past few years. Despite recent evidence for sink limitations to plant growth, there are reasons to be cautious about a sink-limited world view. First, source-limited vegetation models do a reasonable job at capturing geographic patterns in plant productivity and responses to resource limitations. Second, from an evolutionary perspective, it is nonadaptive for plants to invest in increasing carbon assimilation if growth is primarily sink-limited. In this review, we synthesize the potential evidence for and underlying physiology of sink limitation across terrestrial ecosystems and contrast mechanisms of sink limitation with those of source-limited productivity. We highlight evolutionary restrictions on the magnitude of sink limitation at the organismal level. We also detail where mechanisms regulating sink limitation at the organismal and ecosystem scale (e.g. the terrestrial carbon sink) diverge. Although we find that there is currently no direct evidence for widespread organismal sink limitation, we propose a series of follow-up growth chamber manipulations, systematized measurements, and modeling experiments targeted at diagnosing nonadaptive buildup of excess nonstructural carbohydrates that will help illuminate the prevalence and magnitude of organismal sink limitation., (© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)

Erastin, as an effective ferroptosis inducer, has received extensive attention in anti-tumor research. To develop an oral nanocarrier for high efficient loading hydrophobic erastin, here we prepared a fluoro-liposome (FA-3 F-LS) by the self-assembly of the folic acid modified fluorinated amphiphiles-FA-3 F conjugates. The hydrophobic component of three perfluorooctyl chains endows the FA-3 F-LSs with high stability to resist the harsh gastrointestinal tract condition. Folic acids conjugated on the surface of FA-3 F-LSs ensure the better tumor targeting and higher oral bioavailability (32.1%) of erastin-loaded FA-3 F-LSs (erastin@FA-3 F-LSs) than free erastin (8.98%). As targeted anti-tumor nanomedicines, erastin@FA-3 F-LSs effectively inhibited the tumor cell proliferation in vitro by inducing ferroptosis through enhancing the glutathione (GSH) depletion, lipid peroxidation and generation of reactive oxygen species. In vivo studies demonstrated that FA-3 F-LSs displayed excellent application potential as a tumor-targeted oral drug delivery nanocarrier to depress the tumor growth with the loaded chemotherapeutic agents., Competing Interests: Declarations. Ethics approval and consent to participate: All animal studies were conducted according to the Guidelines for Animal Experimentation of Liaocheng University and the Animal Ethics Committee of the institution approved the protocol (Ethics approval No.: 2023022701). Consent for publication: I, Min Hong, give my consent on behalf of all authors for the publication of identifiable details, which can include photographs or details within the text to be published in the Journal of Drug Delivery and Translational Research. Competing interests: The authors have no relevant financial or non-financial interests to disclose., (© 2024. Controlled Release Society.)

This study investigated the effect of various levels of OH-MWCNTs mediated seed priming on germination, growth, and biochemical responses of Indian mustard (Brassica juncea (L.) Czern. & Coss.). Germination efficiency declined significantly up to 33.8% with increasing concentration of OH-MWCNT; however, other growth characteristics including seedling length and weight exhibited a substantial increase of up to 61.6% and 142%, respectively, at moderate levels (≤ 100 µg mL -1 ). Higher doses (250 µg mL -1 , 500 µg mL -1 ) of OH-MWCNT exhibited a toxic effect on all growth parameters resulting in ~ 20% and 55% decline in seedling length and biomass, respectively. Similar trend was recorded in chlorophyll content, and maximum total chlorophyll content (13 ± 3 mg/g FW) was noted at 100 µg/mL OH-MWCNT. Antioxidant enzyme activities including catalase (CAT), guaiacol peroxidase (GPX), polyphenol oxidase (PPO), and superoxide dismutase (SOD) also exhibited similar pattern such that all activities were maximum at 100 µgmL -1 in both shoots and roots and were significantly higher in shoots than in roots. Total phenol content and DPPH activity were also maximum at 100 µgmL -1 , and both parameters were comparatively higher in roots than in shoots. GC-MS analysis revealed that lipids constituted the majority of the proportion of secondary metabolites detected in non-polar extracts of both shoots and roots. Further, correlation analysis established a significant correlation between various growth, physiological, and biochemical parameters. These findings therefore indicated that moderate levels of OH-MWCNT concentrations can enhance B. juncea growth and biochemical responses, which in turn promotes biosynthesis of valuable antioxidant compounds. This study highlighs the potential of MWCNTs as innovative growth enhancers, wherein the higher concentrations may have detrimental effects, therefore, emphasizing the need for careful management of OH-MWCNT usage for agricultural purposes., Competing Interests: Declarations. Ethical approval: Not applicable. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Biochar and SiO 2 NPs are effective soil conditioners, but the impacts and mechanisms of combined application in oilseed rape are not yet clear. Therefore, an experiment was designed to investigate oilseed rape growth, physiological indexes, and transcriptome sequencing under four treatments: control (CK), Platanus orientalis L. leaf biochar (B), SiO 2 NPs (S), and BS. Our results showed that B, S and BS treatments all promoted the root growth, root activity and biomass of oilseed rape, especially the root length and fresh weight in BS, which were increased by 77.48% and 279.07%, respectively. Moreover, the three-dimensional fluorescence spectra of B and BS were similar, and the tyrosine-like substance proportion in B, S and BS increased from 7.8 to 9.4%, 10.2% and 19.5%, respectively. In transcriptome analysis, there were 10,280 differentially expressed genes (DEGs) shared in B and BS, 3431 DEGs shared in S and BS, and 2815 DEGs shared in B, S and BS. We also found that B, S and BS all regulated oilseed rape growth by inducing the lignin biosynthesis and the relevant genes encoding BBE-like, BGL, UDP in the phenylpropanoid biosynthesis pathway. The results provide gene regulation associated with the phenylpropanoid biosynthesis applying the biochar and SiO 2 NPs, which can be used to increase biomass., Competing Interests: Declarations. Competing interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024. The Author(s) under exclusive licence to The Botanical Society of Japan.)

Many insect-induced galls are considered complex structures due to their tissue compartmentalization and multiple roles performed by them. The current study investigates the complex interaction between Nothofagus obliqua host plant and the hymenopteran gall-inducer Espinosa nothofagi, focusing on cell wall properties and cytological features. The E. nothofagi galls present an inner cortex with nutritive and storage tissues, as well as outer cortex with epidermis, chlorenchyma, and water-storing parenchyma. The water-storing parenchyma cells are rich in pectins, heteromannans, and xyloglucans in their walls, and have large vacuoles. Homogalacturonans contribute to water retention, and periplasmic spaces function as additional water reservoirs. Nutritive storage cell walls support nutrient storage, with plasmodesmata facilitating nutrient mobilization crucial for larval nutrition. Their primary and sometimes thick secondary cell walls support structural integrity and act as a carbon reserve. The absent labeling of non-cellulosic epitopes indicates a predominantly cellulosic nature in nutritive cell walls, facilitating larval access to lipid, protein, and reducing sugar-rich contents. The nutritive tissue, with functional chloroplasts and high metabolism-related organelles, displays signs of self-sufficiency, emphasizing its role in larval nutrition and cellular maintenance. Overall, the intricate cell wall composition in E. nothofagi galls showcases adaptations for water storage, nutrient mobilization, and larval nutrition, contributing significantly to our understanding of plant-insect interactions., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Copyright of Journal of South China Agricultural University is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background and Aims: Root exudations play a crucial role in plant nutrient uptake; however, studies on their response to different forms of nitrogen (N) are lacking. To bridge these gaps, we examined the effect of different N forms on the root exudation rates among tree species. Methods: NO3−-N, NH4+-N, and organic N were added to the soil at identical concentrations for three months. Root exudate, fine root, and soil samples were collected from the seedlings of four temperate tree species to determine the root exudation rate, aboveground biomass, root characteristics (morphological, physiological, and nutrient traits), and soil characteristics (soil physicochemical properties and enzymatic activity). The differences in all variables among different N forms and tree species were investigated to explore the causative factors for the variations in root exudation. Results: All three forms of N addition reduced the root exudation rate (up to 43.8%), which was consistent across all species and was positively correlated with root morphological traits and negatively correlated with soil total N concentration. The effect of N addition on root exudation was not affected by N form. Conclusion: For four temperate tree species, inorganic and organic N additions increased soil N availability and reduced root exudation rates, and the effects were not varied with N form. The results indicated that, in the short-term, temperate tree species do not show a preference in utilizing different N resources. These findings provide new insights into the effect of different N form additions on belowground C dynamics and root-soil interactions. [ABSTRACT FROM AUTHOR]

Background and aims: Partitioning the measured net ecosystem carbon dioxide (CO2) exchange into gross primary productivity (GPP) and ecosystem respiration remains a challenge, which scientists try to tackle by using the properties of the trace gas carbonyl sulfide (COS). Its similar pathway into and within the leaf makes it a potential photosynthesis proxy. The application of COS as an effective proxy depends, among other things, on a robust inventory of potential COS sinks and sources within ecosystems. While the soil received some attention during the last couple of years, the role of plant roots is mostly unknown. In our study, we investigated the effects of live roots on the soil COS exchange. Methods: An experimental setup was devised to measure the soil and the belowground plant parts of young beech trees observed over the course of 9 months. Results: During the growing season, COS emissions were significantly lower when roots were present compared to chambers only containing soil, while prior to the growing season, with photosynthetically inactive trees, the presence of roots increased COS emissions. The difference in the COS flux between root-influenced and uninfluenced soil was fairly constant within each month, with diurnal variations in the COS flux driven primarily by soil temperature changes rather than the presence or absence of roots. Conclusion: While the mechanisms by which roots influence the COS exchange are largely unknown, their contribution to the overall ground surface COS exchange should not be neglected when quantifying the soil COS exchange. [ABSTRACT FROM AUTHOR]

Polycyclic aromatic hydrocarbons (PAHs) cause serious stress to biological health and the soil environment as persistent pollutants. Despite the wide use of biochar in promoting soil improvement, the mechanism of biochar removing soil PAHs through rhizosphere effect in the process of phytoremediation remain uncertain. In this study, the regulation of soil niche and microbial degradation strategies under plants and biochar were explored by analyzing the effects of plants and biochar on microbial community composition, soil metabolism and enzyme activity in the process of PAH degradation. The combination of plants and biochar significantly increased the removal of phenanthrene (6.10%), pyrene (11.50%), benzo[a]pyrene (106.02%) and PAHs (27.10%) when compared with natural attenuation, and significantly increased the removal of benzo[a]pyrene (34.51%) and PAHs (5.96%) when compared with phytoremediation. Compared with phytoremediation, the combination of plants and biochar significantly increased soil nutrient availability, enhanced soil enzyme activity (urease and catalase), improved soil microbial carbon metabolism and amino acid metabolism, thereby benefiting microbial resistance to PAH stress. In addition, the activity of soil enzymes (dehydrogenase, polyphenol oxidase and laccase) and the expression of genes involved in the degradation and microorganisms (streptomyces, curvularia, mortierella and acremonium) were up-regulated through the combined action of plants and biochar. In view of the aforementioned results, the combined application of plants and biochar can enhance the degradation of PAHs and alleviate the stress of PAH on soil microorganisms. [ABSTRACT FROM AUTHOR]

In ascomycetous fungi, sexual mate recognition requires interaction of the Ste2 receptor protein produced by one partner with the α-factor peptide pheromone produced by the other partner. In some fungi, Ste2 is further needed for chemotropism towards plant roots to allow for subsequent infection and colonization. Here, we investigated whether this is also true for the pine pitch canker fungus, Fusarium circinatum, which is a devastating pathogen of pine globally. Ste2 knockout mutants were generated for two opposite mating-type isolates, after which all strains were subjected to chemotropism assays involving exudates from pine seedling roots and synthetic α-factor pheromone, as well as a range of other compounds for comparison. Our data show that Ste2 is not required for chemotropism towards any of these other compounds, but, in both wild-type strains, Ste2 deletion resulted in the loss of chemotropism towards pine root exudate. Also, irrespective of mating type, both wild-type strains displayed positive chemotropism towards α-factor pheromone, which was substantially reduced in the deletion mutants and not the complementation mutants. Taken together, these findings suggest that Ste2 likely has a key role during the infection of pine roots in production nurseries. Our study also provides a strong foundation for exploring the role of self-produced and mate-produced α-factor pheromone in the growth and overall biology of the pitch canker pathogen. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista del Centro de Investigación Flamenco Telethusa is the property of Centro de Investigacion Flamenco Telethusa and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista del Centro de Investigación Flamenco Telethusa is the property of Centro de Investigacion Flamenco Telethusa and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Siderophore-dependent iron uptake is a mechanism by which microorganisms scavenge and utilize iron for their survival, growth, and many specialized activities, such as pathogenicity. The siderophore biosynthetic system PubABC in Shewanella can synthesize a series of distinct siderophores, yet how it is regulated in response to iron availability remains largely unexplored. Here, by whole genome screening we identify TCS components histidine kinase (HK) BarA and response regulator (RR) SsoR as positive regulators of siderophore biosynthesis. While BarA partners with UvrY to mediate expression of pubABC post-transcriptionally via the Csr regulatory cascade, SsoR is an atypical orphan RR of the OmpR/PhoB subfamily that activates transcription in a phosphorylation-independent manner. By combining structural analysis and molecular dynamics simulations, we observe conformational changes in OmpR/PhoB-like RRs that illustrate the impact of phosphorylation on dynamic properties, and that SsoR is locked in the 'phosphorylated' state found in phosphorylation-dependent counterparts of the same subfamily. Furthermore, we show that iron homeostasis global regulator Fur, in addition to mediating transcription of its own regulon, acts as the sensor of iron starvation to increase SsoR production when needed. Overall, this study delineates an intricate, multi-tiered transcriptional and post-transcriptional regulatory network that governs siderophore biosynthesis. Regulation of siderophore biosynthesis in Shewanella occurs at both transcriptional and post-transcriptional levels by two TCSs, one of which is composed of an orphan phosphorylation-independent RR functioning via a novel mechanism. [ABSTRACT FROM AUTHOR]

Plant-associated microbes play vital roles in promoting plant growth and health, with plants secreting root exudates into the rhizosphere to attract beneficial microbes. Exudate composition defines the nature of microbial recruitment, with different plant species attracting distinct microbiota to enable optimal adaptation to the soil environment. To more closely examine the relationship between plant genotype and microbial recruitment, we analysed the rhizosphere microbiomes of landrace (Chevallier) and modern (NFC Tipple) barley (Hordeum vulgare) cultivars. Distinct differences were observed between the plant-associated microbiomes of the 2 cultivars, with the plant-growth promoting rhizobacterial genus Pseudomonas substantially more abundant in the Tipple rhizosphere. Striking differences were also observed between the phenotypes of recruited Pseudomonas populations, alongside distinct genotypic clustering by cultivar. Cultivar-driven Pseudomonas selection was driven by root exudate composition, with the greater abundance of hexose sugars secreted from Tipple roots attracting microbes better adapted to growth on these metabolites and vice versa. Cultivar-driven selection also operates at the molecular level, with both gene expression and the abundance of ecologically relevant loci differing between Tipple and Chevallier Pseudomonas isolates. Finally, cultivar-driven selection is important for plant health, with both cultivars showing a distinct preference for microbes selected by their genetic siblings in rhizosphere transplantation assays. Plants secrete root exudates into the rhizosphere to attract beneficial microbes. This study shows that barley genotype exerts multi-level selective pressure on the soil Pseudomonas population, affecting overall abundance, genotype and individual genetic features as well as expression of specific genes. [ABSTRACT FROM AUTHOR]

We investigated the impacts of spherical and triangular-plate-shaped lipid-coated silver nanoparticles (AgNPs) designed to prevent surface oxidation and silver ion (Ag+) dissolution in a small-scale microcosm to examine the role of shape and surface functionalization on biological interactions. Exposures were conducted in microcosms consisting of algae, bacteria, crustaceans, and fish embryos. Each microcosm was exposed to one of five surface chemistries within each shape profile (at 0, 0.1, or 0.5 mg Ag/L) to investigate the role of shape and surface composition on organismal uptake and toxicity. The hybrid lipid-coated AgNPs did not result in any significant release of Ag+ and had the most significant toxicity to D. magna, the most sensitive species, although the bacterial population growth rate was reduced in all exposures. Despite AgNPs resulting in increasing algal growth over the experiment, we found no correlation between algal growth and the survival of D. magna, suggesting that the impacts of the AgNPs on bacterial survival influenced algal growth rates. No significant impacts on zebrafish embryos were noted in any exposure. Our results demonstrate that the size, shape, and surface chemistry of AgNPs can be engineered to achieve specific goals while mitigating nanoparticle risks. [ABSTRACT FROM AUTHOR]

Introduction: Bacteria and archaea are important components in shallow lake ecosystems and are crucial for biogeochemical cycling. While the submerged macrophyte loss is widespread in shallow lakes, the effect on the bacteria and archaea in the sediment and water is not yet widely understood. Methods: In this study, 16S rRNA gene sequencing was used to explore the bacteria and archaea in samples taken from the sediment and water in the submerged macrophyte abundant (MA) and submerged macrophyte loss (ML) areas of Caohai Lake, Guizhou, China. Results: The results showed that the dominant bacterial phyla were Proteobacteria and Chloroflexi in the sediment; the dominant phyla were Proteobacteria, Actinobacteriota, and Bacteroidota in the water. The dominant archaea in sediment and water were the same, in the order of Crenarchaeota, Thermoplasmatota, and Halobacterota. Non-metric multidimensional scaling (NMDS) analyses showed that bacterial and archaeal community structures in the water were significantly affected by the loss of submerged macrophytes, but not by significant changes in the sediment. This suggests that the loss of submerged macrophytes has a stronger effect on the bacterial and archaeal community structures in water than in sediment. Furthermore, plant biomass (PB) was the key factor significantly influencing the bacterial community structure in water, while total nitrogen (TN) was the main factor significantly influencing the archaeal community structure in water. The loss of submerged macrophytes did not significantly affect the alpha diversity of the bacterial and archaeal communities in either the sediment or water. Based on network analyses, we found that the loss of submerged macrophytes reduced the connectivity and complexity of bacterial patterns in sediment and water. For archaea, network associations were stronger for MA network than for ML network in sediment, but network complexity for archaea in water was not significantly different between the two areas. Discussion: This study assesses the impacts of submerged macrophyte loss on bacteria and archaea in lakes from microbial perspective, which can help to provide further theoretical basis for microbiological research and submerged macrophytes restoration in shallow lakes. [ABSTRACT FROM AUTHOR]

This review describes the mechanisms of natural coagulants. It provides a good understanding of the two key processes of coagulation-flocculation: adsorption and charge neutralization, as well as adsorption and bridging. Various factors have influence the coagulation/flocculation process, including the effect of pH, coagulant dosage, coagulant type, temperature, initial turbidity, coagulation speed, flocculation speed, coagulation and flocculation time, settling time, colloidal particles, zeta potential, the effects of humic acids, and extraction density are explained. The bio-coagulants derived from plants are outlined. The impact of organic coagulants on water quality, focusing on their effects on the physicochemical parameters of water, heavy metals removal, and bacteriological water quality, is examined. The methods of extraction and purification of plant-based coagulants, highlighting techniques such as solvent extraction and ultrasonic extraction, are discussed. It also examines the parameters that influence these processes. The methods and principles of purification of coagulating agents, including dialysis, freeze-drying, ion exchange, electrophoresis, filtration, and centrifugation, are listed. Finally, it evaluates the sustainability of natural coagulants, focusing on the environmental, technical, and economic aspects of their use. At the end of this review, the readers should have a comprehensive understanding of the mechanisms, selection, extraction, purification, and sustainability of plant-based natural coagulants in water treatment. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background and aims: Food production is threatened by direct climate change effects including drought. Indirect effects, including changes in plant-pathogen dynamics and increased susceptibility to pathogens, further exacerbate the risks. Root exudation, which plays a crucial role in plant defence against drought and pathogens, is influenced by both water stress and pathogens. However, the interactive effects of these abiotic and biotic factors are rarely studied. Methods: We conducted a controlled environment experiment to investigate the effects of moderate drought and simulated pathogen attack (using pipecolic acid, an inducer of systemic acquired resistance) on the rates of root exudation of total organic carbon (TOC) and total nitrogen (TN) of four tomato cultivars grown in potting soil. Results: Drought increased the exudation of TOC and TN per unit of root area, while pipecolic acid did not have any significant effect. Furthermore, there was no interaction observed between the abiotic and biotic factors. However, due to the reduction in plant and root biomass caused by drought, the total exudation per plant remained similar between control and water-limited plants. Additionally, pipecolic acid reduced the carbon-to-nitrogen ratio of exudates and increased the total exudation of TN. Conclusion: The increased exudation observed in drought-stressed plants may serve as a strategy to maintain root and rhizosphere activity despite reduced root growth. Notably, the impact of drought differed among the tested cultivars, highlighting their diverse levels of drought tolerance. This emphasises the importance of preserving a wide range of crop cultivars to ensure food security under increasing drought. [ABSTRACT FROM AUTHOR]

Nanodynamic therapy (NDT) exerts its anti-tumor effect by activating nanosensitizers to generate large amounts of reactive oxygen species (ROS) in tumor cells. NDT enhances tumor-specific targeting and selectivity by leveraging the tumor microenvironment (TME) and mechanisms that boost anti-tumor immune responses. It also minimizes damage to surrounding healthy tissues and enhances cytotoxicity in tumor cells, showing promise in cancer treatment, with significant potential. This review covers the research progress in five major nanodynamic therapies: photodynamic therapy (PDT), electrodynamic therapy (EDT), sonodynamic therapy (SDT), radiodynamic therapy (RDT), and chemodynamic therapy (CDT), emphasizing the significant role of advanced nanotechnology in the development of NDT for anti-tumor purposes. The mechanisms, effects, and challenges faced by these NDTs are discussed, along with their respective solutions for enhancing anti-tumor efficacy, such as pH response, oxygen delivery, and combined immunotherapy. Finally, this review briefly addresses challenges in the clinical translation of NDT. [ABSTRACT FROM AUTHOR]

Copyright of Biotechnology Bulletin is the property of Biotechnology Bulletin Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The ever-increasing impact of global climate change has profoundly affected crop growth, physiology, and yield characteristics, making them an economic hazard. Canola, a vital crop, is particularly susceptible to environmental challenges at any stage of development, which can have a negative impact on its growth, physiology, and pod formation, ultimately resulting in pod shattering and reduced yield. Canola's rate of return is decreased by mechanical harvesting, which results in pod shattering. The establishment of an abscission layer, triggered by enzymatic and hormonal disruptions, and accompanied by a decline in cell wall stickiness, exacerbates pod shattering. In this context, the potential roles of Paclobutrazol and silver nanoparticles in reinforcing pod strength to withstand shattering. The specific doses of both AgNPs and Paclobutrazol could be done on the aerial parts of plant. These elements facilitate the physiological growth of crops by mitigating the adverse effects of stress on canola, thereby reducing pod shattering and enhancing yield output. This critical review aims to comprehensively explore the physiological, enzymatic, and hormonal-based factors contributing to pod shattering under environmental stresses, while also highlighting the potential mitigation techniques employing Paclobutrazol and silver nanoparticles, to elevate canola yield production. Based on this comprehensive review, it is recommended to organize research experiment to explore the potential of AgNPs and Paclobutrazol in mitigating the pod shattering based yield losses in canola. [ABSTRACT FROM AUTHOR]

The strains of the genus Microbacterium, withmore than 150 species, inhabit diverse environments; plant-associated bacteria reveal their plant growth-promoting activities due to a number of beneficial characteristics. Through the performance of diverse techniques and methods, including isolation of a novel Microbacterium strain from the aerial roots of leafless epiphytic orchid, Chiloschista parishii Seidenf., itsmorphological and biochemical characterization, chemotaxonomy, phylogenetic and genome analysis, as well as bioassays and estimation of its auxin production capacity, a novel strain of ET2T is described. Despite that it shared 16S rRNA gene sequence similarity of 99.79% with Microbacterium kunmingense JXJ CY 27-2T, so they formed a monophyletic group on phylogenetic trees, the two strains showed clear divergence of their genome sequences. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values of ET2T differed greatly from phylogenetically close JXJ CY 27-2T. Based on the differences being below the threshold for species similarity, together with the unique chemotaxonomic characteristics, strain ET2T represents a novel species of the genus Microbacterium. Several genes, putatively involved in auxin biosynthesis were predicted. This strain revealed obvious plant growth-promoting activities, including diazotrophy and biosynthesis of tryptophan-dependent auxins (indole-3-acetic and indole-3-pyruvic acids). Microbial auxins directly stimulated the rhizogenesis, so that the ET2T-inoculated seeds of wheat, cucumber and garden cress showed evident promotion in their growth and development, both under optimal and under cold stress conditions. Based on phenotypic, chemotypic and genotypic evidences, the strain ET2T belongs to the genus Microbacterium, order Micrococcales, class Actinomycetes, and it represents a novel species, for which the name Microbacterium albopurpureum sp. nov. is proposed, with strain ET2T (VKPM Ac-2212, VKM Ac-2998) as the type strain. [ABSTRACT FROM AUTHOR]

Mixing with different broadleaf trees into the monocultures of Cunninghamia lanceolata is widely adopted as an efficient transformation of the pure C. lanceolata forest. However, it is unclear how native broad-leaved trees influence the belowground ecological environment of the pure C. lanceolata culture plantation in nutrient-poor soil of South China. Herein, we aimed to investigate how a long-time mixing with native broadleaf trees shape soil microbial community of the pure C. lanceolata forest across different soil depth (0-20cm and 20-40cm) and to clarify relationships between the modified soil microbial community and those affected soil chemical properties. Using high-throughput sequencing technology, microbial compositions from the mixed C. lanceolata-broadleaf forest and the pure C. lanceolata forest were analyzed. Network analysis was utilized to investigate correlations among microorganisms, and network robustness was assessed by calculating network natural connectivity. Results demonstrated that the content of soil microbial biomass carbon and nitrogen, total phosphorus and pH in mixed forest stand were significantly higher than those in pure forest stand, except for available phosphorus in topsoil (0-20cm). Simultaneously, the mixed C. lanceolata-broadleaf forest has a more homogeneous bacterial and fungal communities across different soil depth compared with the pure C. lanceolata forest, wherein the mixed forest recruited more diverse bacterial community in subsoil (20-40cm) and reduced the diversity of fungal community in topsoil. Meanwhile, the mixed forest showed higher bacterial community stability while the pure forest showed higher fungal community stability. Moreover, bacterial communities showed significant correlations with various soil chemical indicators, whereas fungal communities exhibited correlations with only TP and pH. Therefore, the mixed C. lanceolata-broadleaf forest rely on their recruiting bacterial community to enhance and maintain the higher nutrient status of soil while the pure C. lanceolata forest rely on some specific fungi to satisfy their phosphorus requirement for survive strategy. [ABSTRACT FROM AUTHOR]

The increasing global demand for food, coupled with concerns about the environmental impact of synthetic fertilizers, underscores the urgency of developing sustainable agricultural practices. Nitrogen-fixing bacteria, known as diazotrophs, offer a potential solution by converting atmospheric nitrogen into bioavailable forms, reducing the reliance on synthetic fertilizers. However, a deeper understanding of their interactions with plants and other microbes is needed. In this study, we introduce a recently developed label-free 3D quantitative phase imaging technology called dynamic quantitative oblique back-illumination microscopy (DqOBM) to assess the functional dynamic activity of diazotrophs in vitro and in situ. Our experiments involved three different diazotrophs (Sinorhizobium meliloti, Azotobacter vinelandii, and Rahnella aquatilis) cultured on media with amendments of carbon and nitrogen sources. Over 5 days, we observed increased dynamics in nutrient-amended media. These results suggest that the observed bacterial dynamics correlate with their metabolic activity. Furthermore, we applied qOBM to visualize microbial dynamics within the root cap and elongation zone of Arabidopsis thaliana primary roots. This allowed us to identify distinct areas of microbial infiltration in plant roots without the need for fluorescent markers. Our findings demonstrate that DqOBM can effectively characterize microbial dynamics and provide insights into plant-microbe interactions in situ, offering a valuable tool for advancing our understanding of sustainable agriculture. [ABSTRACT FROM AUTHOR]

Plant–microbe interactions play a crucial role in shaping plant health and survival. In recent years, the role of extracellular vesicles (EVs) in mediating intercellular communication between plants and microbes has emerged as an intriguing area of research. EVs serve as important carriers of bioactive molecules and genetic information, facilitating communication between cells and even between different organisms. Pathogenic bacteria leverage extracellular vesicles (EVs) to amplify their virulence, exploiting their cargo rich in toxins and virulence factors. Conversely, beneficial microbes initiate EV secretion to stimulate plant immune responses and nurture symbiotic relationships. The transfer of EV-packed small RNAs (sRNAs) has been demonstrated to facilitate the modulation of immune responses. Furthermore, harnessing the potential of EVs holds promise for the development of innovative diagnostic tools and sustainable crop protection strategies. This review highlights the biogenesis and functions of EVs in bacteria and their importance in plant defense, and paves the way for future research in this exciting field. [ABSTRACT FROM AUTHOR]

Water deficit (WD) promotes great losses in agriculture, and the development of new sustainable technologies to mitigate the effects of this stress on plants is essential. This study aimed to evaluate the morphophysiological and biochemical alterations induced by the priming of tomato seeds with different formulations in plants under field capacity and WD conditions. In the first experiment, the treatments consisted of nanoparticles of alginate/chitosan and chitosan/tripolyphosphate containing gibberellic acid (GA3) in different concentrations (0.5, 5, and 50 µg mL−1 GA3), in addition to control with deionized water. The alginate/chitosan (5 µg mL−1 GA3) provided the greatest gains in plant growth under field capacity. In addition, under WD this treatment reduced damage to photosystem II (−14%), stomatal conductance (−13%), and water loss (−38%) and increased the instantaneous carboxylation efficiency (+24%) and intrinsic water use efficiency (+12%). In the second experiment, the treatments were alginate/chitosan nanoparticles containing GA3 (NPGA3 5 µg mL−1), free GA3 (GA3 5 µg mL−1), nanoparticles without GA3 (NP), deionized water (WATER), and non-primed seeds (CONT). Under WD, GA3 and CONT maintained plant growth and lost water rapidly, reducing stomatal conductance (−87%) and net photosynthesis (−69%). In contrast, NPGA3 decreased leaf area (−44%) and increased root-to-shoot ratio (+39%) when compared to GA3, reducing water loss (−28%). Activation of protective mechanisms (e.g., superoxide dismutase and catalase activities) by WATER, NPGA3, and NP treatments also resulted in lower susceptibility to WD compared to CONT and GA3. The results highlight the positive effect of seed priming on plant response to WD, which was enhanced by the use of nanoencapsulated GA3. [ABSTRACT FROM AUTHOR]

In this study, the impacts of long-term soil and crop management practices on crop productivity and soil health in oilseed brassica-based production systems were examined. Different tillage, crop residue recycling and cropping systems (fallow–mustard, cluster bean–mustard, green gram–mustard, maize–mustard, pearl millet–mustard and sesame–mustard) were studied for 5 years at two soil depths (0–15 and 15–30 cm) in a split-plot design with three replications. No-till permanent beds with crop residue (PB + R) noticeably improved soil organic carbon (SOC), microbial biomass carbon (MBC), enzymes (dehydrogenase (DHA) and alkaline phosphatase (AlP)), nitrogen fractions (available and total nitrate) and available phosphorus and potassium content in both soil layers compared to conventional tillage without crop residues. However, the plough soil layer (0–15 cm) showed higher concentrations of soil carbon, enzymes, N fractions and available P than in the subsoil (15–30 cm). The dynamic soil biological and chemical properties also varied with the crop stage, and higher MBC at 30 days, SOC and enzymatic activities at 60 days, and N fractions and available P and K during the harvesting of mustard crop were recorded. Green gram–mustard rotation showed higher values in terms of biological and chemical parameters. Thus, the legume-based mustard crop rotation following no-till permanent beds and residue recycling was found to be holistic in terms of improving soil health and nutrient cycling. [ABSTRACT FROM AUTHOR]

Dickeya solani, belonging to the Soft Rot Pectobacteriaceae, are aggressive necrotrophs, exhibiting both a wide geographic distribution and a wide host range that includes many angiosperm orders, both dicot and monocot plants, cultivated under all climatic conditions. Little is known about the infection strategies D. solani employs to infect hosts other than potato (Solanum tuberosum L.). Our earlier study identified D. solani Tn5 mutants induced exclusively by the presence of the weed host S. dulcamara. The current study assessed the identity and virulence contribution of the selected genes mutated by the Tn5 insertions and induced by the presence of S. dulcamara. These genes encode proteins with functions linked to polyketide antibiotics and polysaccharide synthesis, membrane transport, stress response, and sugar and amino acid metabolism. Eight of these genes, encoding UvrY (GacA), tRNA guanosine transglycosylase Tgt, LPS-related WbeA, capsular biosynthesis protein VpsM, DltB alanine export protein, glycosyltransferase, putative transcription regulator YheO/PAS domain-containing protein, and a hypothetical protein, were required for virulence on S. dulcamara plants. The implications of D. solani interaction with a weed host, S. dulcamara, are discussed. [ABSTRACT FROM AUTHOR]

Nitric oxide (NO) is a multifunctional gaseous signal that modulates the growth, development and stress tolerance of higher plants. NO donors have been used to boost plant endogenous NO levels and to activate NO-related responses, but this strategy is often hindered by the relative instability of donors. Alternatively, nanoscience offers a new, promising way to enhance NO delivery to plants, as NO-releasing nanomaterials (e.g. S-nitrosothiol-containing chitosan nanoparticles) have many beneficial physicochemical and biochemical properties compared to non-encapsulated NO donors. Nano NO donors are effective in increasing tissue NO levels and enhancing NO effects both in animal and human systems. The authors believe, and would like to emphasize, that new trends and technologies are essential for advancing plant NO research and nanotechnology may represent a breakthrough in traditional agriculture and environmental science. Herein, we aim to draw the attention of the scientific community to the potential of NO-releasing nanomaterials in both basic and applied plant research as alternatives to conventional NO donors, providing a brief overview of the current knowledge and identifying future research directions. We also express our opinion about the challenges for the application of nano NO donors, such as the environmental footprint and stakeholder's acceptance of these materials., (© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)

Immunoglobulins are an essential part of the humoral immune response. IgG4 antibodies are the least prevalent subclass and have unique structural and functional properties. In this review, we discuss IgG4 class switch and B cell production. We review the importance of IgG4 antibodies in the context of allergic responses, helminth infections and malignancy. We discuss their anti-inflammatory and tolerogenic effects in allergen-specific immunotherapy, and ability to evade the immune system in parasitic infection and tumour cells. We then focus on the role of IgG4 autoantibodies and autoantigens in IgG4-autoimmune diseases and IgG4-related disease, highlighting important parallels and differences between them. In IgG4-autoimmune diseases, pathogenesis is based on a direct role of IgG4 antibodies binding to selfantigens and disturbing homeostasis. In IgG4-related disease, where affected organs are infiltrated with IgG4-expressing plasma cells, IgG4 antibodies may also directly target a number of self-antigens or be overexpressed as an epiphenomenon of the disease. These antigen-driven processes require critical T and B cell interaction. Lastly, we explore the current gaps in our knowledge and how these may be addressed. [ABSTRACT FROM AUTHOR]

Agricultural nanotechnology has considerable promise for addressing global agricultural production/security, biodiversity, and global warming issues. Current trends in publications and patents demonstrate that biotechnology technologies, particularly for crops, are being developed to improve agricultural productivity and disease management. In the current issue, we strongly advocate for the use of biosynthesized nanoparticles from a variety of sources, including plants, agricultural waste, and microbes, as a prerequisite for significant and in-depth study. Nanomaterials offer a wide range of practical uses in agriculture, including nanofertilizers, nanopesticides, nanoherbicides, nanosensors, and smart delivery systems for controlled agrochemical release. Additionally, nano-tools are employed for plant breeding and genetic manipulation. A thorough examination of the physicochemical soil properties of the agricultural fields where nanoparticles will be used will aid in minimizing their impact on plant and soil biota. Finally, and most importantly, we strongly recommend the inclusion of nanotoxicity, legislation, biosafety, and risk assessment as the top priorities when developing regulatory policies to address biosafety concerns. Starting today, thorough efforts must be carried out to advance and develop futuristic work based on recognized knowledge shortages. [ABSTRACT FROM AUTHOR]

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The priming of seeds is shown as a viable technique to improve germination, the growth of the radicle and plumule, and the seedling vigor index, which gives rise to seedlings with higher quality and tolerance to environmental growing conditions. Sodium selenite (Na2SeO3) and selenium nanoparticles (nSe) were used as priming media and postgermination biostimulation in seeds of jalapeño pepper, in concentrations of 1, 5, 10, 15, 20, 25, 30, 35, 40, and 45 mg L−1 for the two Se species, and control treatment. This research aimed to determine the priming response of jalapeño pepper regarding the germination percent, germination speed index, radicle length, plumule length, fresh weight, and seedling vigor index. The stimulation and phytotoxicity thresholds were also computed. The results showed a percentage of germination greater than 80% in all concentrations evaluated. Most variables of jalapeño pepper presented stimulation responses at Na2SeO3 doses lower than 5 mg L−1 and nSe doses lower than 15 mg L−1. The higher daily germination was favored by nSe on the fifth day compared to the sixth day of Na2SeO3; in addition, the higher cumulative germination occurred on the sixth day with nSe and on the eighth day with Na2SeO3. The use of low Na2SeO3 concentrations positively favors germination and the morphological traits of the shoots. Likewise, the use of Se in nanometric form was friendlier, that is, the degree of tolerance to Se was higher. [ABSTRACT FROM AUTHOR]

Legumes are a diverse and important group of plants that play a vital role in agriculture, food security, and environmental sustainability. Rhizobia are symbiotic bacteria that form nitrogen-fixing nodules on legume roots, providing the plant with a valuable source of nitrogen. Phenolic acids are a group of secondary metabolites produced by plants that have a wide range of biological functions, including defense against pests and diseases, tolerance to abiotic stresses, and nutrient uptake. In the context of climate change and the imperative for sustainable agriculture, this study delves into the dynamic responses of legume species to varying light intensities and their intricate interactions with soil microorganisms. We investigated the impact of light intensity and rhizobial inoculation on the biomass, nitrate reductase, acid phosphatase, and production of phenolic acids in the roots of four legume species, Trifolium repens, Vicia sativa, Ornithopus compressus, and Coronilla juncea. Plants were grown under three light intensity regimes (low, medium, and high) and inoculated with either rhizobia or a non-inoculated control. The results highlight that shaded-light-adapted species, T. repens and V. sativa, increased root exudate production when exposed to high light intensity. This response aligns with their mining strategy, effectively allocating resources to optimize nutrient acquisition under varying conditions. In contrast, species hailing from well-illuminated environments, O. compressus and C. juncea, displayed distinct strategies by significantly increasing biomass under high irradiance, capitalizing on the available light and nutrients. The mining strategy of legumes emerged as a central theme, influencing biomass production, nitrogen dynamics, and enzymatic activities. The strong correlations between biomass and total nitrogen accumulation underscore the role of the mining strategy in efficient nutrient acquisition. Inoculated plants, which rely more on biological nitrogen fixation (BNF), exhibited lower δ15N values, indicative of a successful mining strategy to acquire and utilize atmospheric nitrogen. Enzymatic activities and phenolic acids exhibited significant interspecies variations, reflecting the adaptability of legumes to different light conditions. The findings of this study could be used to develop new strategies for improving legume stress tolerance, nutrient uptake capacity, and rhizosphere health. [ABSTRACT FROM AUTHOR]

Climate and land management changes are altering carbon inputs to soil. The consequence of such input changes on long‐term soil organic carbon (SOC) balance depends on the transit behavior of carbon inputs. Using observational carbon input and radiocarbon data in global soil profiles, we reveal that on average nearly 25% of new entering carbon leave soil in 1 year irrespective of entering depth, and the remained fraction after 30 years is only ∼13%. Nevertheless, the majority of SOC is older than 30 years in all soil depths. Together, these results demonstrate low transfer efficiency of carbon inputs to aged SOC which is the meaningful carbon component for long‐term SOC sequestration. Additionally, we reveal that SOC aging and carbon input transiting are two distinct processes, which should be simultaneously, but mechanistical‐separately, considered to predict and manage SOC dynamics in response to carbon input changes under climate and land management changes. Plain Language Summary: Soil contains a lot of carbon, and its levels can change based on climate and how we manage the land. Our global soil research shows that when new carbon goes into the soil, about 25% of it leaves within a year, regardless of depth. After 30 years, only about 13% remains. Interestingly, most of the carbon in the soil is older than 30 years, showing that it can store carbon for a long time. We found that transferring carbon inputs to aged soil organic carbon (SOC) is not very efficient. This aged SOC is crucial for long‐term carbon storage, which can help combat climate change. We also discovered that SOC aging and carbon input transit are separate processes. To manage soil carbon well in response to climate and land changes, we need to consider these processes separately but at the same time. In conclusion, our research reveals how carbon behaves in soil and stresses the importance of aged SOC for long‐term carbon storage. Understanding these processes better can lead to more effective strategies for managing and protecting our soils, contributing to the fight against climate change. Key Points: Around 80% of carbon inputs leave soil in 10 years after entering and do not contribute to aged soil carbon componentThe low transfer efficiency of carbon inputs to aged soil carbon undermines the feasibility of managing carbon inputs for soil carbon sequestrationThe dynamics of new carbon inputs and aged soil carbon are influenced by distinct climatic and edaphic attributes [ABSTRACT FROM AUTHOR]

Terrestrial ecosystems are increasinglybeing exposedtometallicnanoparticles (MNPs). However, the global-scale impact of MNPs on soil carbon dioxide (CO2) emission remains unknown, limiting our understanding of the role of MNPs in global carbon cycle. We compiled a dataset comprising 1764 pairs of experimental observations to investigate the effects of MNPs exposure on soil respiration parameters, including respiration rates, related enzyme activities, and microbial variables. We found that MNPs could stimulate or suppress soil basal respiration rates across global environments, depending largely on the type of MNPs. We further showed that, although the inhibition of MNPs on most enzyme activities peaked in short-term exposure (≤28 days), prolonged exposure to MNPs still inhibited soil organic carbon degradation. Finally, we determined that environmental conditions (e.g., soil pH and presence/absence of plants) were also important regulators of the influence of MNPs on soil respiration parameters. Our findings underline that the effects of MNPs on soil CO2 emission depended on MNPs type, exposure design, and environmental factors, which is critical for predicting the role of MNPs in influencing the global carbon cycle and climate change. [ABSTRACT FROM AUTHOR]

Terrestrial ecosystems are increasingly being exposed to metallic nanoparticles (MNPs). However, the global‐scale impact of MNPs on soil carbon dioxide (CO2) emission remains unknown, limiting our understanding of the role of MNPs in global carbon cycle. We compiled a dataset comprising 1764 pairs of experimental observations to investigate the effects of MNPs exposure on soil respiration parameters, including respiration rates, related enzyme activities, and microbial variables. We found that MNPs could stimulate or suppress soil basal respiration rates across global environments, depending largely on the type of MNPs. We further showed that, although the inhibition of MNPs on most enzyme activities peaked in short‐term exposure (≤28 days), prolonged exposure to MNPs still inhibited soil organic carbon degradation. Finally, we determined that environmental conditions (e.g., soil pH and presence/absence of plants) were also important regulators of the influence of MNPs on soil respiration parameters. Our findings underline that the effects of MNPs on soil CO2 emission depended on MNPs type, exposure design, and environmental factors, which is critical for predicting the role of MNPs in influencing the global carbon cycle and climate change. [ABSTRACT FROM AUTHOR]

In today's time, agricultural productivity is severely affected by climate change and increasing pollution. Hence, several biotechnological approaches, including genetic and non-genetic strategies, have been developed and adapted to increase agricultural productivity. One of them is nano-priming, i.e., seed priming with nanomaterials. Thus far, nano-priming methods have been successfully used to mount desired physiological responses and productivity attributes in crops. In this review, the literature about the utility of nano-priming methods for increasing seed vigor, germination, photosynthetic output, biomass, early growth, and crop yield has been summarized. Moreover, the available knowledge about the use of nano-priming methods in modulating plant antioxidant defenses and hormonal networks, inducing salinity tolerance and disease resistance, as well as alleviating heavy metal toxicity in plants, is reviewed. The significance of nano-priming methods in the context of phytotoxicity and environmental safety has also been discussed. For future perspectives, knowledge gaps in the present literature are highlighted, and the need for optimization and validation of nano-priming methods and their plant physiological outcomes, from lab to field, is emphasized. [ABSTRACT FROM AUTHOR]

Soil salinization is a common environmental problem that seriously threatens crop yield and food security, especially through its impact on seed germination. Nanoparticle priming, an emerging seed treatment method, is receiving increasing attention in improving crop yield and stress resistance. This study used alfalfa seeds as materials to explore the potential benefits of cerium oxide nanoparticle (CeO2NP) priming to promote seed germination and improve salt tolerance. CeO2NPs at concentrations up to 500 mg/L were able to significantly alleviate salt stress in alfalfa seeds (200 mM), with 50 mg/L of CeO2NP having the best effect, significantly (P< 0.05) increasing germination potential (from 4.0% to 51.3%), germination rate (from 10.0% to 62.7%), root length (from 8.3 cm to 23.1 cm), and seedling length (from 9.8 cm to 13.7 cm). Priming treatment significantly (P< 0.05) increased seed water absorption by removing seed hardness and also reducing abscisic acid and jasmonic acid contents to relieve seed dormancy. CeO2NP priming increased α-amylase activity and osmoregulatory substance level, decreased reactive oxygen species and malonaldehyde contents and relative conductivity, and increased catalase enzyme activity. Seed priming regulated carotenoid, zeatin, and plant hormone signal transduction pathways, among other metabolic pathways, while CeO2NP priming additionally promoted the enrichment of α-linolenic acid and diterpenoid hormone metabolic pathways under salt stress. In addition, CeO2NPs enhanced α-amylase activity (by 6.55%) in vitro. The optimal tested concentration (50 mg/L) of CeO2NPs was able to improve the seed vigor, enhance the activity of α-amylase, regulate the osmotic level and endogenous hormone levels, and improve the salt tolerance of alfalfa seeds. This study demonstrates the efficacy of a simple seed treatment strategy that can improve crop stress resistance, which is of great importance for reducing agricultural costs and promoting sustainable agricultural development. [ABSTRACT FROM AUTHOR]

Soil degradation of the spoil ground generated during the construction of expressways is a serious concern, which affects the surrounding ecological environment. In this study, the combination of vetiver grass cultivation and fertilization was used to repair the soil in spoil ground. Therefore, 12 treatments were set up to evaluate the effects of ecological restoration, including no fertilization (CK), nitrogen fertilizer only (N1:150 kg/ha, N2:300 kg/ha, and N3:450 kg/ha), phosphorus fertilizer only (P1:350 kg/ha and P2:700 kg/ha), and a chemical fertilizer combination (NP: N1P1, N2P1, N3P1, N1P2, N2P2, N3P2). Compared with the CK, the fertilization treatments significantly increased the contents of soil ammonia nitrogen, soil total nitrogen, soil total phosphorus, and soil available phosphorus in rhizosphere soil increased by 6.91–48.49%, 1.36–153.81%, 63.40–333.44%, and 3.22–138.12%, respectively; the activity of β-xylosidase and N-acetyl-glucosaminidase increased significantly by 41.25–119.84% and 13.54–128.45%, respectively; and the Chao1 and observed species microbial diversity indices increased by 19.98–55.62% and 16.49–53.29%, respectively. In addition, NP treatment had a higher path coefficient for plant traits [NP (0.897) > P (0.767) > N (0.373)] and enzyme activities [NP (0.807) > N (0.703) > P (− 0.993)]. Furthermore, N2P2 had the highest comprehensive score (34), indicating that N2P2 could be used as an effective fertilizer combination. These results indicate that combined fertilization treatment (NP) improved the path coefficient of soil physicochemical properties to plant traits and soil enzyme activities, and thus better restored the habitats of spoil ground. [ABSTRACT FROM AUTHOR]

Nanoparticle transport into plants is an evolving field of research with diverse applications in agriculture and biotechnology. This article provides an overview of the challenges and prospects associated with the transport of nanoparticles in plants, focusing on delivery methods and the detection of nanoparticles within plant tissues. Passive and assisted delivery methods, including the use of roots and leaves as introduction sites, are discussed, along with their respective advantages and limitations. The barriers encountered in nanoparticle delivery to plants are highlighted, emphasizing the need for innovative approaches (e.g., the stem as a new recognition site) to optimize transport efficiency. In recent years, research efforts have intensified, leading to an evendeeper understanding of the intricate mechanisms governing the interaction of nanomaterials with plant tissues and cells. Investigations into the uptake pathways and translocation mechanisms within plants have revealed nuanced responses to different types of nanoparticles. Additionally, this article delves into the importance of detection methods for studying nanoparticle localization and quantification within plant tissues. Various techniques are presented as valuable tools for comprehensively understanding nanoparticle–plant interactions. The reliance on multiple detection methods for data validation is emphasized to enhance the reliability of the research findings. The future outlooks of this field are explored, including the potential use of alternative introduction sites, such as stems, and the continued development of nanoparticle formulations that improve adhesion and penetration. By addressing these challenges and fostering multidisciplinary research, the field of nanoparticle transport in plants is poised to make significant contributions to sustainable agriculture and environmental management. [ABSTRACT FROM AUTHOR]

In this paper, a viability study is conducted for a bioremediation intervention in a cultivated area contaminated by a pipeline oil spill. In this context, green technologies such as bioremediation and phytoremediation could represent an optimal solution for reducing pollution without deteriorating soil quality. The phytoremediation test was conducted at the microcosm scale using three plant species (Zea mays, Lupinus albus and Medicago sativa) and at the mesocosm scale (Zea mays), also evaluating the application of plant growth-promoting bacteria (PGPB). The results showed that the selected plants, being able to grow satisfactorily, are able to lessen the presence of hydrocarbons in the soil. An increase of 15–18% in the degradation of the C > 12 fractions in vegetated soils was observed, confirming the effect of plants on the biodegradation of hydrocarbons in the soil. Moreover, a further improvement was recorded after adding PGPB, resulting in fresh biomass production being up to 50% higher than the controls and the degradation of the C > 12 fraction increasing by up to an additional 10%. Particular attention was also paid to pyrene, considered an indicator of PAH contamination. At the end of the experimentation in vegetated soils, pyrene removal reached values above 50%. By favoring plant growth, the addition of PGPB resulted in a further up to 20% reduction in the content of the contaminant in the soil. The primary role of the plants in soil contaminated by petroleum derivatives was to accelerate the degradation of contaminants through the stimulation of microbial activity. Therefore, the cooperation between plants and microorganisms can be concretely used as a nature-based solution in a sustainable and economical way. [ABSTRACT FROM AUTHOR]

Background: The transcription factors (TFs) in thermophilic cyanobacteria might represent a uniquely evolved gene repertoire in light of the strong selective pressure caused by hostile habitats. Understanding the molecular composition of the TF genes in thermophilic cyanobacteria will facilitate further studies regarding verifying their exact biochemical functions and genetic engineering. However, limited information is available on the TFs of thermophilic cyanobacteria. Herein, a thorough investigation and comparative analysis were performed to gain insights into the molecular composition of the TFs in 22 thermophilic cyanobacteria. Results: The results suggested a fascinating diversity of the TFs among these thermophiles. The abundance and type of TF genes were diversified in these genomes. The identified TFs are speculated to play various roles in biological regulations. Further comparative and evolutionary genomic analyses revealed that HGT may be associated with the genomic plasticity of TF genes in Thermostichus and Thermosynechococcus strains. Comparative analyses also indicated different pattern of TF composition between thermophiles and corresponding mesophilic reference cyanobacteria. Moreover, the identified unique TFs of thermophiles are putatively involved in various biological regulations, mainly as responses to ambient changes, may facilitating the thermophiles to survive in hot springs. Conclusion: The findings herein shed light on the TFs of thermophilic cyanobacteria and fundamental knowledge for further research regarding thermophilic cyanobacteria with a broad potential for transcription regulations in responses to environmental fluctuations. [ABSTRACT FROM AUTHOR]

Pseudomonas aeruginosa is a highly pathogenic bacterium known for its ability to sense and coordinate the production of virulence factors in response to host immune responses. However, the regulatory mechanisms underlying this process have remained largely elusive. In this study, we investigate the two-component system CprRS in P. aeruginosa and unveil the crucial role of the sensor protein CprS in sensing the human host defense peptide LL-37, thereby modulating bacterial virulence. We demonstrate that CprS acts as a phosphatase in the presence of LL-37, leading to the phosphorylation and activation of the response regulator CprR. The results prove that CprR directly recognizes a specific sequence within the promoter region of the HigBA toxin-antitoxin system, resulting in enhanced expression of the toxin HigB. Importantly, LL-37-induced HigB expression promotes the production of type III secretion system effectors, leading to reduced expression of proinflammatory cytokines and increased cytotoxicity towards macrophages. Moreover, mutations in cprS or cprR significantly impair bacterial survival in both macrophage and insect infection models. This study uncovers the regulatory mechanism of the CprRS system, enabling P. aeruginosa to detect and respond to human innate immune responses while maintaining a balanced virulence gene expression profile. Additionally, this study provides new evidence and insights into the complex regulatory system of T3SS in P. aeruginosa within the host environment, contributing to a better understanding of host-microbe communication and the development of novel strategies to combat bacterial infections. Author summary: Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that commonly causes fatal infections in immunosuppressed individuals. However, the underlying mechanisms that enable P. aeruginosa to adapt to capricious environments of the human body are still poorly understood. Recent studies unveil that two-component systems (TCSs) play a vital role in pathogenesis by sensing environmental cues and modulating gene expression in P. aeruginosa. In this study, we identified the CprRS, one of TCSs in P. aeruginosa, as an essential virulence factor. The sensor protein CprS directly interacts with human host defense peptide LL-37 and activates the response regulator CprR, which then coordinates the activities of type III secretion system for higher cytotoxicity. Loss of cprS or cprR significantly impairs bacterial survival in macrophage and host colonization. Collectively, this work contributes to explain the potent role of CprRS in the molecular-level of host-microbe communication to efficiently survival within the human host. [ABSTRACT FROM AUTHOR]

This study reports the effects of a computationally informed and avocado-seed mediated Phyto engineered CuS nanoparticles as fertilizing agent on the ionome and amino acid metabolome of Pinto bean seeds using both bench top and ion beam analytical techniques. Physico-chemical analysis of the Phyto engineered nanoparticles with scanning-electron microscopy, transmission electron microscopy, X-ray diffraction, and Fourier Transform Infrared Spectroscopy confirmed the presence of CuS nanoparticles. Molecular dynamics simulations to investigate the interaction of some active phytocompounds in avocado seeds that act as reducing agents with the nano-digenite further showed that 4-hydroxybenzoic acid had a higher affinity for interacting with the nanoparticle's surface than other active compounds. Seeds treated with the digenite nanoparticles exhibited a unique ionome distribution pattern as determined with external beam proton-induced X-ray emission, with hotspots of Cu and S appearing in the hilum and micropyle area that indicated a possible uptake mechanism via the seed coat. The nano-digenite also triggered a plant stress response by slightly altering seed amino acid metabolism. Ultimately, the nano-digenite may have important implications as a seed protective or nutritive agent as advised by its unique distribution pattern and effect on amino acid metabolism. [ABSTRACT FROM AUTHOR]

Background: The rhizosheath, a cohesive soil layer firmly adhering to plant roots, plays a vital role in facilitating water and mineral uptake. In pearl millet, rhizosheath formation is genetically controlled and influenced by root exudates. Here, we investigated the impact of root exudates on the microbiota composition, interactions, and assembly processes, and rhizosheath structure in pearl millet using four distinct lines with contrasting soil aggregation abilities. Results: Utilizing 16S rRNA gene and ITS metabarcoding for microbiota profiling, coupled with FTICR-MS metabonomic analysis of metabolite composition in distinct plant compartments and root exudates, we revealed substantial disparities in microbial diversity and interaction networks. The ß-NTI analysis highlighted bacterial rhizosphere turnover driven primarily by deterministic processes, showcasing prevalent homogeneous selection in root tissue (RT) and root-adhering soil (RAS). Conversely, fungal communities were more influenced by stochastic processes. In bulk soil assembly, a combination of deterministic and stochastic mechanisms shapes composition, with deterministic factors exerting a more pronounced role. Metabolic profiles across shoots, RT, and RAS in different pearl millet lines mirrored their soil aggregation levels, emphasizing the impact of inherent plant traits on microbiota composition and unique metabolic profiles in RT and exudates. Notably, exclusive presence of antimicrobial compounds, including DIMBOA and H-DIMBOA, emerged in root exudates and RT of low aggregation lines. Conclusions: This research underscores the pivotal influence of root exudates in shaping the root-associated microbiota composition across pearl millet lines, entwined with their soil aggregation capacities. These findings underscore the interconnectedness of root exudates and microbiota, which jointly shape rhizosheath structure, deepening insights into soil–plant-microbe interactions and ecological processes shaping rhizosphere microbial communities. Deciphering plant–microbe interactions and their contribution to soil aggregation and microbiota dynamics holds promise for the advancement of sustainable agricultural strategies. 46mkfZGLAk7WD-UFCK_cLi Video Abstract [ABSTRACT FROM AUTHOR]