179 results on '"Samanta, Luna"'
Search Results
152. Harnessing the potential of dialdehyde alginate-xanthan gum hydrogels as niche bioscaffolds for tissue engineering.
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Jena, Soumya Ranjan, Dalei, Ganeswar, Das, Subhraseema, Nayak, Jasmine, Pradhan, Manoranjan, and Samanta, Luna
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XANTHAN gum , *TISSUE engineering , *HYDROGELS , *ESCHERICHIA coli , *WESTERN immunoblotting , *P53 antioncogene - Abstract
Biomimetic hydrogels composed of natural polysaccharides have invariably blossomed as niche biomaterials in tissue engineering applications. The prospects of creating an extracellular matrix (ECM)-like milieu from such hydrogels has garnered considerable importance. In this study, we have fabricated bioscaffolds comprising dialdehyde alginate and xanthan gum and explored their potential use in tissue regeneration. The fabricated scaffolds displayed an interconnected porous network structure that is highly desirable for the aforesaid application. The scaffolds were endowed with good mechanical properties, thermostability, protein adsorption efficacy and degradability. Curcumin-loaded hydrogels exhibited appreciable antibacterial activity against E. coli. In vitro cytocompatibility studies revealed that the scaffolds promoted adhesion and proliferation of 3T3 fibroblast cells. The Western blot analysis of p53 gene indicated no growth arrest or apoptosis in 3T3 cells thus, signifying the non-toxic nature of the scaffolds. Furthermore, the ECM formation was confirmed via SDS-PAGE analysis. The overall results clearly validated these scaffolds as effectual biomaterials for tissue engineering applications. [Display omitted] • Biopolymeric scaffolds of xanthan gum and dialdehyde alginate synthesized and characterized. • Scaffolds possessed an interconnected porous network structure. • Scaffolds endowed with good mechanical properties, thermostability, protein adsorption efficacy and degradation behaviour. • Scaffolds promoted proliferation of 3T3 cells and ECM formation. [ABSTRACT FROM AUTHOR]
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- 2022
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153. Control of invasive apple snails and their use as pollutant ecotoxic indicators: a review.
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Panda, Falguni, Pati, Samar Gourav, Bal, Abhipsa, Das, Kajari, Samanta, Luna, and Paital, Biswaranjan
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POLLUTANTS , *SNAILS , *CYPERMETHRIN , *COPPER sulfate , *GLUTATHIONE peroxidase , *OXYGEN consumption , *SUPEROXIDE dismutase , *ORCHARDS - Abstract
Apple snails are one of the most survived fresh water invasive species that causes massive economic loss to vegetations, especially to the rice fields. They survive against many environmental stressors and are considered as a major pest in water-lodged cultivated area due to their intrusiveness, adaptation mechanisms and survivability. Therefore, there is a need for strategies to control their population. Snails can also be used as pollution bioindicators. Here we review apple snails for their ecotoxic management without compromising environment and co-inhabitants, and their use as semi-sessile invasive ecotoxic marker species. Snails have been found to accumulate 0.19–0.21% biphenyl ethers, 1.65% copper sulphate with 26.7% morality, 1.53–29.7% ivermectin, 43% polybrominated biphenyl ethers, 59% triphenylphosphine oxide, 8–100% of various heavy metals and nanoparticles. Feed intake of apple snails can be enhanced up to 22% with copper supplements, while 17 and 100% mortality can be achieved with niclosamide monohydrate (0.13 mg/L) and tea seed derivatives (0.015 g/L) exposure, respectively. Snails respond to stress factors with up to 29.6% higher lipid peroxides and 1.06% protein carbonyls, along with up to 80, 240 and 127% higher activities of superoxide dismutase, catalase and glutathione peroxidase enzymes, respectively, after cypermethrin exposure. Oppositely, mercury (2–8 mg/L) and aldicarb can reduce their oxygen consumption and acetylcholinesterase activity of up to 88.1 and 96.08%, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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154. What Does the Future Hold
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Bertolla, Ricardo P., Agarwal, Ashok, Samanta, Luna, Bertolla, Ricardo P., Durairajanayagam, Damayanthi, and Intasqui, Paula
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- 2016
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155. Proteomics
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Bertolla, Ricardo P., Agarwal, Ashok, Samanta, Luna, Bertolla, Ricardo P., Durairajanayagam, Damayanthi, and Intasqui, Paula
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- 2016
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156. Tracking research trends and hotspots in sperm DNA fragmentation testing for the evaluation of male infertility: a scientometric analysis.
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Baskaran, Saradha, Agarwal, Ashok, Panner Selvam, Manesh Kumar, Finelli, Renata, Robert, Kathy Amy, Iovine, Concetta, Pushparaj, Peter Natesan, Samanta, Luna, Harlev, Avi, and Henkel, Ralf
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MALE infertility , *DNA , *SPERMATOZOA , *ANDROLOGY , *FERTILIZATION (Biology) , *OXIDATIVE stress - Abstract
Background: This article describes the research trends in sperm DNA fragmentation (SDF) over the past 20 years (1999–2018) using a scientometric approach. Methods: A stepwise approach was adopted to retrieve scientometric data (articles per year, authors, affiliations, journals, countries) from Scopus and analyze the publication pattern of SDF with reference to key areas of research in the field of Andrology. Results: A total of 2121 articles were retrieved related to SDF. Our data revealed an increasing research trend in SDF (n = 33 to n = 173) over the past 20 years (R2 = 0.894). Most productive country in publications was the USA (n = 450), while Agarwal A. (n = 129) being the most productive author. Most of the articles in SDF were primarily focused on lifestyle (n = 157), asthenozoospermia (n = 135) and varicocele (130). Mechanistic studies on SDF were published twice as much as prognostic/diagnostic studies, with significant emphasis on oxidative stress. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was the most widely used technique to evaluate SDF. Publications on SDF related to assisted reproductive techniques also showed a linear increasing trend (R2 = 0.933). Conclusions: Our analysis revealed an increasing trend in SDF publications predominantly investigating lifestyle, asthenozoospermia and varicocele conditions with TUNEL being the most widely used technique. A substantial increase in research is warranted to establish SDF as prognostic/diagnostic parameter to evaluate clinical scenarios and ART outcomes. [ABSTRACT FROM AUTHOR]
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- 2019
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157. NIR-Triggered SrSnO3 -photosynthesized nanocatalyst: A potent anticancer agent against triple negative breast cancer cells.
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Pradhan, Anwesha, Behera, Debendra, Jena, Soumya Ranjan, Soren, Siba, Parhi, Purnendu, and Samanta, Luna
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TRIPLE-negative breast cancer , *NANOPARTICLES , *CANCER cells , *FIELD emission electron microscopy , *ANTINEOPLASTIC agents , *GLUTATHIONE - Abstract
• SrSnO 3 photosensitive nanocatalyst was prepared by simple sol-gel method. • NIR irradiation of SrSnO 3 nanoparticles generate holes with oxidative ability. • Holes facilitated production of ROS in vitro inducing death of TNBC cancer cells. • SrSnO 3 nanophotocatalyst shows excellent drug-free hole therapy against TNBC. The major systemic therapeutic option for Triple Negative Breast Cancer (TNBC) is chemotherapy. TNBC often responds well to chemo, however, chemotherapy carries toxic adverse effects and content-limited interventional efficiency. Thereby, a drug-free treatment paradigm is imperative, in which synthetic polymers spontaneously display efficient anticancer efficacy without the use of conventional chemotherapeutic agents. Aiming at the goal to treat TNBC, SrSnO 3 nanoparticles were devised as a nanocatalyst to accomplish near infrared (NIR) photocatalytic production of oxidative holes for achieving hole therapy. An inexpensive sol-gel method was employed in this study to synthesize SrSnO 3 nanoparticle using strontium chloride hexahydrate and stannous chloride dihydrate. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS) analyses were used to characterize the synthesized SrSnO 3 nanoparticles while Density Functional Theory (DFT) was used to study the nanoparticle at atomistic level and the band gap was calculated to be 1.84 eV using Perdew−Burke−Ernzerhof (PBE) functionals. The anticancer effect of SrSnO 3 nanoparticle was tested against TNBC cell line MDA-MB-231 at different concentration (10–50 μg/mL) with near infrared (NIR) irradiation while cells without any treatment or treatment with only SrSnO 3 or NIR served as controls. Under NIR irradiation, the IC 50 value was found to be 40 μg/mL achieving 50% cell death with augmented release of reactive oxygen species (ROS) over time. Reduced and oxidized glutathione (GSH and GSSG, respectively) serve as cellular redox buffers and protect cells from ROS mediated damage. Nearly 18.6% reduced glutathione (GSH) was found to be consumed with 5 min of NIR irradiation that was augmented to 66.5% of GSH when exposure time was increased to 10 min suggesting a prooxidant mediated death of cancer cell line. The results suggested SrSnO 3 nanoparticle is a potent photocatalyst when triggered by NIR and can enable highly effective and biosafe hole-mediated treatment for TNBC. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2023
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158. Constitutively active follicle-stimulating hormone receptor enables androgen-independent spermatogenesis.
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Oduwole, Olayiwola O., Peltoketo, Hellevi, Poliandri, Ariel, Vengadabady, Laura, Chrusciel, Marcin, Doroszko, Milena, Samanta, Luna, Owen, Laura, Keevil, Brian, Rahman, Nafis A., and Huhtaniemi, Ilpo T.
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SPERMATOGENESIS , *GENE expression , *TESTIS , *MOLECULAR genetics , *LABORATORY mice - Abstract
Spermatogenesis is regulated by the 2 pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This process is considered impossible without the absolute requirement of LH-stimulated testicular testosterone (T) production. The role of FSH remains unclear because men and mice with inactivating FSH receptor (FSHR) mutations are fertile. We revisited the role of FSH in spermatogenesis using transgenic mice expressing a constitutively strongly active FSHR mutant in a LH receptor-null (LHR-null) background. The mutant FSHR reversed the azoospermia and partially restored fertility of Lhr-/- mice. The finding was initially ascribed to the residual Leydig cell T production. However, when T action was completely blocked with the potent antiandrogen flutamide, spermatogenesis persisted. Hence, completely T-independent spermatogenesis is possible through strong FSHR activation, and the dogma of T being a sine qua non for spermatogenesis may need modification. The mechanism for the finding appeared to be that FSHR activation maintained the expression of Sertoli cell genes considered androgen dependent. The translational message of our findings is the possibility of developing a new strategy of high-dose FSH treatment for spermatogenic failure. Our findings also provide an explanation of molecular pathogenesis for Pasqualini syndrome (fertile eunuchs; LH/T deficiency with persistent spermatogenesis) and explain how the hormonal regulation of spermatogenesis has shifted from FSH to T dominance during evolution. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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159. In situ crosslinked dialdehyde guar gum-chitosan Schiff-base hydrogels for dual drug release in colorectal cancer therapy.
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Dalei, Ganeswar, Das, Subhraseema, Ranjan Jena, Soumya, Jena, Debasis, Nayak, Jasmine, and Samanta, Luna
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IRINOTECAN , *GUAR gum , *COLORECTAL cancer , *HYDROGELS , *CANCER treatment , *GUAR , *COLON cancer - Abstract
[Display omitted] • In situ crosslinked chitosan-dialdehyde guar gum Schiff-base hydrogels synthesized. • Dialdehyde guar gum was accomplished by periodate oxidation of guar gum. • Curcumin and aspirin dual drug release from hydrogels was optimum in colorectal pH. • Combined formulation showed enhanced anticancer effects in HT-29 colon cancer cells. • Hydrogels were potent for simultaneous chemotherapy and pain relief in CRC therapy. This work advocates that in situ crosslinked chitosan-dialdehyde guar gum (CsDAGG) hydrogels can be effectually used for dual drug release in colorectal cancer therapy. Herein, we designed various grades of CsDAGG hydrogels where DAGG was accomplished by periodate oxidation of guar gum. With increasing DAGG content, a diminution in swelling and a more compact matrix was evident. In this study, curcumin in combination with aspirin were chosen for investigation in colon carcinoma. The hydrogels ensured the protection of the drugs from absorption in stomach and small intestine and enabled a controlled release at the targeted site of colorectum. The exposure of the aforesaid combined drug formulation further demonstrated enhanced anticancer effects in HT-29 colon cancer cells. The findings altogether suggested the high potency of the developed hydrogels for the co-administration of drugs that can provide chemotherapy and pain relief simultaneously in colorectal cancer combination therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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160. Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NFĸB/AKT/mTOR/KEAP1 signalling in rat heart.
- Author
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Mishra, Pallavi, Paital, Biswaranjan, Jena, Srikanta, Swain, Shasank S., Kumar, Sunil, Yadav, Manoj K., Chainy, Gagan B. N., and Samanta, Luna
- Abstract
Oxidative stress is implicated in both hypo- and hyper-thyroid conditions. In the present study an attempt has been made to elucidate possible interaction between vitamin E or/and curcumin (two established antioxidants) with active portion (redox signaling intervening region) of nuclear factor erythroid 2-related factor 2 (NRF2) as a mechanism to alleviate oxidative stress in rat heart under altered thyroid states. Fifty Wistar strain rats were divided into two clusters (Cluster A: hypothyroidism; Cluster B: hyperthyroidism). The hypo- (0.05% (w/v) propylthiouracil in drinking water) and hyper- (0.0012% (w/v) T4 in drinking water) thyroid rats in both clusters were supplemented orally with antioxidants (vitamin E or/and curcumin) for 30 days. Interactive least count difference and principal component analyses indicated increase in lipid peroxidation, reduced glutathione level, alteration in the activities and protein expression of antioxidant enzymes like superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase under altered thyroid states. However, the expression of stress survival molecules; nuclear factor κB (NFκB) and the serine-threonine kinase B (Akt), in hyper-thyroidism only points towards different mechanisms responsible for either condition. Co-administration of vitamin E and curcumin showed better result in attenuating expression of mammalian target for rapamycin (mTOR), restoration of total protein content and biological activity of Ca2+ ATPase in hyperthyroid rats, whereas, their individual treatment showed partial restoration. Since NRF2 is responsible for activation of antioxidant response element and subsequent expression of antioxidant enzymes, possible interactions of both vitamin E or/and curcumin with the antioxidant enzymes, NRF2 and its regulator Kelch ECH associating protein (KEAP1) were studied in silico. For the first time, a modeled active portion of the zipped protein NRF2 indicated its interaction with both vitamin E and curcumin. Further, curcumin and vitamin E complex showed in silico interaction with KEAP1. Reduction of oxidative stress by curcumin and/or vitamin E may be due to modulation of NRF2 and KEAP1 function in rat heart under altered thyroid states. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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161. Bioactivity guided isolation of antidiabetic and antioxidant compound from Xylocarpus granatum J. Koenig bark.
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Das, Swagat Kumar, Samantaray, Dibyajyoti, Sahoo, Sudhir Kumar, Pradhan, Sukanta Kumar, Samanta, Luna, and Thatoi, Hrudayanath
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SILICA gel , *NORMAL-phase chromatography , *GLUCOSIDASES , *BARK , *MANGROVE plants , *BINDING energy , *HYDROGEN peroxide - Abstract
The present study was designed to identify antidiabetic and antioxidant constituents from ethanol bark extract of a medicinally important mangrove plant Xylocarpus granatum J. Koenig, using in vitro bioactivity-guided fractionation. The repeated fractionation of X. granatum ethanol bark extract (XGEB) by silica gel column chromatography yielded a compound with strong antidiabetic and antioxidant potential. The bioactive compounds likely to be present in the XGEB fraction were identified by FT-IR, 1H & 13C NMR and MS analysis and determined as a limonoid derivative Xyloccensin-I, by comparing spectral data with the literature reports. The isolated compound demonstrated excellent in vitro antidiabetic potential IC50 values of 0.25 and 0.16 mg/ml, respectively for α-amylase and α-glucosidase inhibition study. The antioxidant potential assayed by DPPH, ABTS, superoxide and hydrogen peroxide scavenging studies exhibited that the isolated compound could scavenge these free radicals with IC50 values of 0.041, 0.039, 0.096 and 0.235 mg/ml, respectively. Further, in silico study was performed to find the antidiabetic activity of Xyloccensin-I by docking it against α-glucosidase enzyme. The study demonstrated that Xyloccensin-I have satisfactory interactions and binding energies when docked into target which further confirms the possible mode of antidiabetic action of the isolated compound. The bioactivity assays further asserts the antidiabetic and antioxidant efficacy of the isolated compound which strongly suggests that Xyloccensin-I holds promise in the pharmaceutical industry. The results from this study provide new mechanistic evidence justifying, at least in part, the traditional use of X. granatum extract for antidiabetic and antioxidants activities. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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162. Anthracene-Conjugated Steroidal Amphiphiles: Soft Functional Materials Exhibiting Supramolecular Aggregation Induced Enhanced Emission with Potential Applications as Drug Carriers and Fluorescent Bioprobes.
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Kuldeep, Jena SR, Samanta L, and Subuddhi U
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- Humans, Bile Acids and Salts chemistry, Surface-Active Agents chemistry, Surface-Active Agents chemical synthesis, Steroids chemistry, Anthracenes chemistry, Fluorescent Dyes chemistry, Fluorescent Dyes chemical synthesis, Drug Carriers chemistry
- Abstract
Bile salts (BS) are naturally occurring steroidal biosurfactants. The ease of functionalization of BSs has boosted their use as inexpensive building blocks for the fabrication of a broad set of value-added soft functional materials. In the present work, three fluorescent bile acid (FBA) derivatives have been synthesized by conjugating anthracene at the side chain of lithocholic acid, deoxycholic acid, and cholic acid to understand the effect of the nature of the steroid nucleus on their physicochemical properties. In an aqueous medium, the FBAs showed a strong supramolecular aggregation propensity, even in the micromolar concentration range, which is in contrast to their BS analogues that form micelles mostly in the millimolar range. The FBA aggregation leads to a prearranged geometry in the ground state with a favorable orientation of anthracene units for excimer formation on excitation, leading to supramolecular aggregation-induced enhanced emission (AIEE). A detailed investigation reveals the pivotal role of the steroidal skeleton in their aggregation propensity and optical behavior. The FBA assemblies, with ordered structures plus anthracene being a part of their building blocks, are endowed with interesting properties different from those in dilute organic media, which makes them extremely attractive for diverse applications, e.g., as potential drug carriers owing to their ability to serve as efficient hosts for the protective encapsulation of hydrophobic guests; as membrane probes and bioimaging agents due to their efficient membrane permeability and cell-imaging ability; and as system probes because of their remarkable sensitivity toward the aggregation process of natural bile salts in the aqueous medium. Therefore, the present study not only enhances the fundamental understanding of this unique class of amphiphiles but also opens new prospects in tailoring novel self-assembled soft functional materials. Moreover, it offers a benchmark for developing BS-based fluorescent derivatives with unique photophysical characteristics for applications as potential bioprobes.
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- 2025
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163. Curcumin-loaded nanoparticles effectively prevent T 4 -induced oxidative stress in rat heart.
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Mishra P, Sahoo DK, Mohanty C, and Samanta L
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- Animals, Rats, Male, Reactive Oxygen Species metabolism, Antioxidants pharmacology, Antioxidants chemistry, Antioxidants metabolism, Myocardium metabolism, Myocardium pathology, Heart drug effects, Lipid Peroxidation drug effects, Curcumin pharmacology, Curcumin chemistry, Oxidative Stress drug effects, Nanoparticles chemistry, Rats, Wistar
- Abstract
In this study, we report the cardioprotective effect of the glycerol monooleate (GMO) based nanocurcumin in both in vitro and in vivo conditions under a hyperthyroid state. The heart is one of the primary target organs sensitive to the action of thyroid hormone, and slight variations in the thyroid hormone serum concentrations result in measurable changes in cardiac performance. Hyperthyroidism-induced hypermetabolism is associated with oxidative stress and is an important mechanism responsible for the progression of heart failure. Curcumin has been known to play a protective role against oxidative stress-related diseases like Alzheimer's, asthma, and aging due to its antioxidant properties. Nevertheless, its potent biological activity has been hindered due to its poor bioavailability. To overcome this drawback, a GMO-based biodegradable nanoparticle (NP) formulation loaded with curcumin has been developed, and the protective effect of curcumin-loaded NPs was compared against the native drug. Oxidative stress parameters like reactive oxygen species (ROS) release, change in mitochondrial membrane permeability, lipid peroxidation (LPx), lactate dehydrogenase (LDH) release, and the activity and protein expression of the endogenous antioxidant enzymes like superoxide dismutase, catalase (CAT) and glutathione peroxidase were evaluated. The results from in vitro showed that curcumin-loaded NPs showed better DPPH and NO radical scavenging activity than native curcumin in a concentrations range of 2.5-20 µM. It was also observed that the nanoparticulate curcumin was comparatively more effective than native curcumin in protecting against ROS-induced membrane damage by reducing LPx and LDH leakage at low concentrations of 5-10 µM. Further, curcumin NPs performed better in facilitating the activities of antioxidant enzymes under in vitro and in vivo conditions with respect to time and concentrations, resulting in reduced cellular ROS levels. In this scenario, we anticipate that curcumin-loaded NPs can serve as a better antioxidant than its native counterpart in protecting the heart from oxidative stress-related diseases., (© 2024 John Wiley & Sons Ltd.)
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- 2024
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164. Spectroscopic insights with molecular docking and molecular dynamic simulation studies of anticancer drug 5-Fluorouracil targeting human pyruvate kinase m2.
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Priyadarshinee M, Dehury B, Mishra S, Jena C, Patra M, Mishra NK, Samanta L, and Mallick BC
- Abstract
This study was conducted to test the efficacy of 5-fluorouracil (5-FU) as an anticancer drug against the human pyruvate kinase isozyme M2 (PKM2) using spectroscopic, molecular docking and molecular dynamic simulation studies. PKM2 fluorescence quenching studies in the presence of 5-FU performed at three different temperatures indicates dynamic quenching processes with single-set of binding ( n ≈ 1) profile. The biomolecular quenching constants ( k
q ) and the effective binding constants ( Kb ) obtained are shown to increase with temperature. The calculated enthalpy (ΔH) and entropy changes (ΔS) are estimated to be -118.06 kJ/mol and 146.14 kJ/mol/K respectively, which suggest the possible mode of interaction as electrostatic and hydrogen bonding. Further, these values were used to estimate the free energy changes (ΔG) and that increases with temperature. The negative ΔG values clearly indicates spontaneous binding process that stabilizes the complex formed between 5-FU and PKM2. Far-UV CD spectra of PKM2 in the presence of 5-FU shows decrease in α-helix contents which point towards the destabilization of secondary structure that weakens the biological activity of PKM2. The intrinsic fluorescence study and circular dichroism (CD) spectra showed minor conformational changes of PKM2 in the presence of 5-FU. Additionally, the results obtained from molecular docking and all-atom molecular dynamic simulation study supports the insight of the spectroscopic binding studies, and strengthens the dynamic stability of the complex between 5-FU and PKM2 through H-bonding. This study establishes a paradigm of 5-FU-PKM2 complexation and the efficacy of 5-FU that compromises the biological activity of the targeted PKM2.Communicated by Ramaswamy H. Sarma.- Published
- 2024
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165. Editorial: Hormonal imbalance-associated oxidative stress and protective benefits of nutritional antioxidants.
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Sahoo DK, Samanta L, Kesari KK, and Mukherjee S
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- Humans, Oxidative Stress, Reactive Oxygen Species, Antioxidants therapeutic use, Antioxidants metabolism, Endocrine System Diseases
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
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- 2024
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166. Editorial: Omics for infertility and contraception: two sides of same coin.
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Jena SR, Mohanty G, Kesari KK, Durairajanayagam D, and Samanta L
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
- Published
- 2023
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167. Human sperm proteome reveals the effect of environmental borne seminal polyaromatic hydrocarbons exposome in etiology of idiopathic male factor infertility.
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Nayak J, Jena SR, Kumar S, Kar S, Dixit A, and Samanta L
- Abstract
Introduction: Polyaromatic hydrocarbons (PAHs) are considered as redox active environmental toxicants inducing oxidative stress (OS) mediated injury to cells. Oxidative predominance is reported in 30%-80% of idiopathic male infertility (IMI) patients. Hence, this work aims to unravel correlation, if any, between seminal PAH exposome and sperm function in IMI patients through a proteomic approach. Methods: Seminal PAH exposome was analyzed in 43 fertile donors and 60 IMI patients by HPLC and receiver operating characteristic (ROC) curve was applied to find out the cut-off limits. Spermatozoa proteome was analyzed by label free liquid chromatography mass spectroscopy (LC-MS/MS) followed by molecular pathway analysis using bioinformatic tools. Validation of key proteins' expression and protein oxidative modifications were analyzed by western blot. Results and discussion: Of the 16 standards toxic PAH, 13 were detected in semen. Impact of the different PAHs on fertility are Anthracene < benzo (a) pyrene < benzo [b] fluoranthene < Fluoranthene < benzo (a) anthracene
ROC ). Benzo [a] pyrene is invariably present in all infertile patients while naphthalene is present in both groups. Of the total 773 detected proteins (Control: 631 and PAH: 717); 71 were differentially expressed (13 underexpressed, 58 overexpressed) in IMI patients. Enrichment analysis revealed them to be involved in mitochondrial dysfunction and oxidative phosphorylation, DNA damage, Aryl hydrocarbon receptor (AHR) signaling, xenobiotic metabolism and induction of NRF-2 mediated OS response. Increased 4-hydroxynonenal and nitrosylated protein adduct formation, and declined antioxidant defense validates induction of OS. Increased GSH/GSSG ratio in patients may be an adaptive response for PAH metabolism via conjugation as evidenced by over-expression of AHR and Heat shock protein 90 beta (HSP90β) in patients. Seminal PAH concentrations, particularly benzo (a) pyrene can be used as a marker to distinguish IMI from fertile ones with 66.67% sensitivity and 100% specificity (95% confidence interval) along with oxidative protein modification and expression of AHR and HSP90β., Competing Interests: SK was employed by Kar Clinic and Hospital Pvt., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Nayak, Jena, Kumar, Kar, Dixit and Samanta.) - Published
- 2023
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168. Spatio-temporal changes in oxidative stress physiology parameters in apple snail Pila globosa as a function of soil Mg, Ca, organic carbon and aquatic physico-chemical factors.
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Pati SG, Panda F, Samanta L, and Paital B
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- Animals, Oxidative Stress, Snails metabolism, Ascorbic Acid metabolism, Antioxidants metabolism, Carbon metabolism
- Abstract
Information on the oxidative stress physiology parameters (OSPP) in general and as a function of the fluctuation of Mg, Ca and organic carbon present in soil and aquatic physico-chemical factors such as pH, temperature and salinity in particular are scanty in the amphibious snail Pila globosa. A spatio-temporal analysis of redox metabolism (as OSPP) followed by discriminant function analysis of the obtained data were performed in P. globosa sampled from the east-coasts of Odisha state, India (mostly along the Bay of Bengal) for environmental health assessment purposes. Results revealed that the OSPP are susceptible to seasonal synergistic variation of soil and physico-chemical factors. Overall, lipid peroxidation, total antioxidant capacity, activities of catalase, glutathione reductase had positive correlation whereas ascorbic acid, the reduced glutathione and the activity of superoxide dismutase had non-significant correlation with the soil Mg, Ca, organic carbon, and pH, temperature and salinity of water. In the summer season, the snails had a marked 51.83% and 26.41% higher lipid peroxidation level and total antioxidative activity as compared to the other seasons. Spatial variation of OSPP indicates that snails residing away from the Bay of Bengal coast had at least 4.4% lower antioxidant level in winter and 30% higher lipid peroxide levels in summer as compared to the rest of the sampling sites. Results on OSPP in P. globosa may be useful for monitoring the ecotoxic effects of environment using molluscs in general and P. globosa in particular., (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
- Published
- 2023
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169. Exploring Steroidal Surfactants as Potential Drug Carriers for an Anticancer Drug Curcumin: An Insight into the Effect of Surfactants' Structure on the Photophysical Properties, Stability, and Activity of Curcumin.
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Mohanty S, Tirkey B, Jena SR, Samanta L, and Subuddhi U
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- Humans, Surface-Active Agents chemistry, Drug Carriers toxicity, Drug Carriers chemistry, Drug Delivery Systems, Micelles, Solubility, Curcumin pharmacology, Curcumin chemistry, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry
- Abstract
Despite having tremendous medicinal benefits, the practical applications of curcumin are limited, owing to two major challenges: poor aqueous solubility and lack of bioavailability. In this regard, biosurfactant-based micellar systems have surged recently for the development of novel and more effective formulations because of their biological relevance. This study deals with a comprehensive and comparative investigation on the effect of seven structurally different steroidal surfactants on the photophysical properties of curcumin and also evaluates these steroidal surfactants as possible drug delivery media for curcumin. The photophysical properties of curcumin exhibited a strong dependence on the structure of the steroidal surfactant; the extent of excited-state proton transfer between curcumin and the surfactants depends strongly on the type of the side chain in the surfactants, which mostly dictates the photophysics of curcumin in the presence of these structural variants. The solubility of curcumin and its stability at different pHs and temperatures and in the presence of salt are significantly enhanced in the presence of these surfactants. Furthermore, the curcumin-loaded micelles exhibited improved intracellular uptake and cytotoxicity against MCF-7 cancer cells than pristine curcumin. Among these steroidal surfactants, CHAPS, the zwitterionic derivative of cholic acid, was the most efficient one to offer better solubility and stability to curcumin under all conditions, and the death rate of MCF-7 cells by curcumin was found to be the highest in the presence of CHAPS, indicating the enhanced bioavailability of curcumin. Therefore, CHAPS-based colloids are found to be promising candidates as potential drug carriers for curcumin.
- Published
- 2023
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170. Paternal factors in recurrent pregnancy loss: an insight through analysis of non-synonymous single-nucleotide polymorphism in human testis-specific chaperone HSPA2 gene.
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Mohanty G, Jena SR, Kar S, and Samanta L
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- Animals, Female, Humans, Male, Mice, Heat-Shock Proteins metabolism, HSP70 Heat-Shock Proteins genetics, HSP70 Heat-Shock Proteins metabolism, Molecular Chaperones genetics, Molecular Chaperones metabolism, Polymorphism, Single Nucleotide, Semen metabolism, Abortion, Habitual genetics, Abortion, Habitual metabolism, Testis metabolism
- Abstract
Heat shock protein A2 (HSPA2) is a testis-specific molecular chaperone of the 70 kDa heat shock protein (HSP70) family and reported to play a key role in spermatogenesis as well as in the remodelling of the sperm surface during capacitation. It is established that mice lacking HSPA2 gene are infertile and spermatozoa that fail to interact with the zona pellucida of the oocyte consistently lack HSPA2 protein expression. However, its role in post fertilization events is not fully understood. Owing to the importance of HSPA2 in male reproduction, the present study is undertaken to reveal the association between genetic mutation and phenotypic variation in recurrent pregnancy loss (RPL) patients through an in silico prediction analysis. In this study, we used different computational tools and servers such as SIFT, PolyPhen2, PROVEAN, nsSNPAnalyzer, and SNPs & GO to analyse the functional consequences of the nsSNPs in human HSPA2 gene. The most damaging amino acid variants generated were subjected to I-Mutant 2.0 and ConSurf. Post-translational modifications such as phosphorylation mediated by these deleterious nsSNPs were analysed using NetPhos 2.0, and gene-gene interaction study was conducted using GeneMANIA. Finally, in-depth studies of the nsSNPs were studied through Project HOPE. The findings of the study revealed 18 nsSNPs to be deleterious using a combinatorial bioinformatic approach. Further functional analysis suggests that screening of nsSNP variants of HSPA2 that tend to be conserved and has potential to undergo phosphorylation at critical positions (rs764410231, rs200951589, rs756852956) may be useful for predicting outcome in altered reproductive outcome. The physicochemical alterations and its impact on the structural and functional conformity were determined by Project HOPE. Gene-gene interaction depicts its close association with antioxidant enzyme (SOD1) strongly supporting an inefficient oxidative scavenging regulatory mechanism in the spermatozoa of RPL patients as reported earlier. The present study has thus identified high-risk deleterious nsSNPs of HSPA2 gene and would be beneficial in the diagnosis and prognosis of the paternal effects in RPL patients., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2022
- Full Text
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171. Impact of Oxidative Stress on Embryogenesis and Fetal Development.
- Author
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Swain N, Moharana AK, Jena SR, and Samanta L
- Subjects
- Pregnancy, Humans, Female, Fetal Development, Oxidative Stress
- Abstract
Multiple cellular processes are regulated by oxygen radicals or reactive oxygen species (ROS) where they play crucial roles as primary or secondary messengers, particularly during cell proliferation, differentiation, and apoptosis. Embryogenesis and organogenesis encompass all these processes; therefore, their role during these crucial life events cannot be ignored, more so when there is an imbalance in redox homeostasis. Perturbed redox homeostasis is responsible for damaging the biomolecules such as lipids, proteins, and nucleic acids resulting in leaky membrane, altered protein, enzyme function, and DNA damage which have adverse impact on the embryo and fetal development. In this article, we attempt to summarize the available data in literature for an in-depth understanding of redox regulation during development that may help in optimizing the pregnancy outcome both under natural and assisted conditions., (© 2022. The Author(s), under exclusive license to Springer Nature Switzerland AG.)
- Published
- 2022
- Full Text
- View/download PDF
172. Pathological Role of Reactive Oxygen Species on Female Reproduction.
- Author
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Goutami L, Jena SR, Swain A, and Samanta L
- Subjects
- Humans, Female, Reactive Oxygen Species, Health Status
- Abstract
Oxidative stress (OS), a clinical predicament characterized by a shift in homeostatic imbalance among prooxidant molecules embracing reactive oxygen species (ROS) and reactive nitrogen species (RNS), along with antioxidant defenses, has been established to play an indispensable part in the pathophysiology of subfertility in both human males and females. ROS are highly reactive oxidizing by-products generated during critical oxygen-consuming processes or aerobic metabolism. A healthy body system has its own course of action to maintain the equilibrium between prooxidants and antioxidants with an efficient defense system to fight against ROS. But when ROS production crosses its threshold, the disturbance in homeostatic balance results in OS. Besides their noxious effects, literature studies have depicted that controlled and adequate ROS concentrations exert physiologic functions, especially that gynecologic OS is an important mediator of conception in females. Yet the impact of ROS on oocytes and reproductive functions still needs a strong attestation for further analysis because the disruption in prooxidant and antioxidant balance leads to abrupt ROS generation initiating multiple reproductive diseases such as polycystic ovary syndrome (PCOS), endometriosis, and unexplained infertility in addition to other impediments in pregnancy such as recurrent pregnancy loss, spontaneous abortion, and preeclampsia. The current article elucidates the skeptical state of affairs created by ROS that influences female fertility., (© 2022. The Author(s), under exclusive license to Springer Nature Switzerland AG.)
- Published
- 2022
- Full Text
- View/download PDF
173. Proteomic Signatures of Sperm Mitochondria in Varicocele: Clinical Use as Biomarkers of Varicocele Associated Infertility.
- Author
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Samanta L, Agarwal A, Swain N, Sharma R, Gopalan B, Esteves SC, Durairajanayagam D, and Sabanegh E
- Subjects
- Adenosine Triphosphate biosynthesis, Adult, Biomarkers analysis, Biomarkers metabolism, Case-Control Studies, Healthy Volunteers, Humans, Infertility, Male etiology, Infertility, Male pathology, Male, Microcirculation, Middle Aged, Mitochondria pathology, Oxidation-Reduction, Oxidative Stress, Prognosis, Protein Interaction Mapping, Proteome metabolism, Proteomics methods, Semen metabolism, Semen Analysis methods, Spermatozoa cytology, Spermatozoa pathology, Testis blood supply, Varicocele complications, Young Adult, Infertility, Male diagnosis, Mitochondria metabolism, Proteome analysis, Spermatozoa metabolism, Varicocele pathology
- Abstract
Purpose: Varicocele may disrupt testicular microcirculation and induce hypoxia-ischemia related degenerative changes in testicular cells and spermatozoa. Superoxide production at low oxygen concentration exacerbates oxidative stress in men with varicocele. Therefore, the current study was designed to study the role of mitochondrial redox regulation and its possible involvement in sperm dysfunction in varicocele associated infertility., Materials and Methods: We identified differentially expressed mitochondrial proteins in 50 infertile men with varicocele and in 10 fertile controls by secondary liquid chromatography-tandem mass spectroscopy data driven in silico analysis. Identified proteins were validated by Western blot and immunofluorescence. Seminal oxidation-reduction potential was measured., Results: We identified 22 differentially expressed proteins related to mitochondrial structure (LETM1, EFHC, MIC60, PGAM5, ISOC2 and import TOM22) and function (NDFSU1, UQCRC2 and COX5B, and the core enzymes of carbohydrate and lipid metabolism). Cluster analysis and 3-dimensional principal component analysis revealed a significant difference between the groups. All proteins studied were under expressed in infertile men with varicocele. Liquid chromatography-tandem mass spectroscopy data were corroborated by Western blot and immunofluorescence. Impaired mitochondrial function was associated with decreased expression of the proteins (ATPase1A4, HSPA2, SPA17 and APOA1) responsible for proper sperm function, concomitant with elevated seminal oxidation-reduction potential in the semen of infertile patients with varicocele., Conclusions: Impaired mitochondrial structure and function in varicocele may lead to oxidative stress, reduced ATP synthesis and sperm dysfunction. Mitochondrial differentially expressed proteins should be explored for the development of biomarkers as a predictor of infertility in patients with varicocele. Antioxidant therapy targeting sperm mitochondria may help improve the fertility status of these patients., (Copyright © 2018 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
174. Dietary supplementation of Spirulina ameliorates iron-induced oxidative stress in Indian knife fish Notopterus Notopterus.
- Author
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Mohanty D and Samanta L
- Subjects
- Animals, Catalase metabolism, Fish Diseases diet therapy, Fishes metabolism, Gills drug effects, Gills metabolism, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Glutathione Transferase metabolism, Iron pharmacokinetics, Iron Overload diet therapy, Iron Overload veterinary, Liver drug effects, Liver metabolism, Male, Muscles drug effects, Muscles metabolism, Sulfhydryl Compounds metabolism, Superoxide Dismutase metabolism, Dietary Supplements, Iron toxicity, Oxidative Stress drug effects, Spirulina
- Abstract
Iron though an essential cofactor for many proteins including haemoglobin and cytochromes, when in excess (>1 ppm in water and 100 ppm in fish tissue) elicits toxicity via Fenton reaction inducing oxidative stress. The present study aimed to evaluate the efficacy of dietary Spirulina (Arthrospira platensis) supplementation on waterborne-iron induced oxidative stress in the tissues of Notopterus notopterus. Juvenile fishes were divided randomly into 4 groups, namely, Group-I: control fed with commercial diet only, Group-II, III and IV treated with 0.75 ppm FeCl
3 where Group-II fed with commercial diet only, Group-III with 10% (w/w) Spirulina supplemented commercial diet and Group-IV with 100% (w/w) Spirulina diet only; for 7 and 28 days (n = 6 per group). Tissue oxidative stress biomarkers like lipid peroxidation (LPx), protein carbonylation (PC) and protein thionylation (protein and nonprotein-SH content); antioxidant defence (superoxide dismutase: SOD; catalase; CAT; glutathione peroxidase/reductase: GPx/GR; glutathione s-transferase: GST; metalothionine: MT and reduced glutathione: GSH) and iron accumulation in the gill, liver and muscles tissue were analysed. The augmented oxidative predominance in the tissues with respect to LPx and PC along with decline in antioxidant defence (SOD, CAT, GPx, GR, GST, MT, PSH, NPSH and GSH) by iron was neutralized by Spirulina supplementation in the diet in a dose and duration dependent manner where 100% Spirulina diet for 28 days completely ameliorated iron-induced oxidative stress in fish tissues. Thus, Spirulina can be used as a dietary supplement for fishes cultured in water bodies with iron overload., (Copyright © 2018. Published by Elsevier B.V.)- Published
- 2018
- Full Text
- View/download PDF
175. The enigmatic seminal plasma: a proteomics insight from ejaculation to fertilization.
- Author
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Samanta L, Parida R, Dias TR, and Agarwal A
- Subjects
- Biomarkers metabolism, Humans, Male, Proteomics, Spermatozoa physiology, Ejaculation, Fertilization, Proteome, Semen metabolism
- Abstract
Background: The 'omics' approach for a noninvasive diagnosis of male reproductive system disorders has gained momentum during the last decade, particularly from a screening and prognosis point of view. Due to the rapid development in assisted reproductive technologies (ART) over the years, the major focus of proteomic studies has been around the ejaculated spermatozoa. Although seminal plasma is not a requirement for ART, the question arose whether the role of seminal plasma is merely to transport spermatozoa., Main Body: Seminal plasma (SP) contains a large diversity of proteins that are essential not only for sperm transport, but also for sperm protection and maturation. Most of the proteins bind to sperm surface through exosomes (epididymosomes and prostasomes), modulating sperm function, interaction with the female reproductive tract and finally fertilization. This review focuses on the state-of-art discoveries regarding SP proteome and its role in fertilization., Conclusion: Tissue-specific proteins in the SP have emerged as fundamental contributors for protein biomarker discovery. This is important for a noninvasive diagnosis of male infertility and development of new therapeutic approaches. Moreover, ART success rates may be improved by taking into account the critical role of seminal proteome in fertilization.
- Published
- 2018
- Full Text
- View/download PDF
176. Multivariate analysis of potential biomarkers of oxidative stress in Notopterus notopterus tissues from Mahanadi River as a function of concentration of heavy metals.
- Author
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Mohanty D and Samanta L
- Subjects
- Animals, Biomarkers metabolism, Environmental Monitoring, India, Male, Multivariate Analysis, Organ Specificity, Rivers chemistry, Water Quality, Environmental Exposure, Fishes metabolism, Metals, Heavy metabolism, Oxidative Stress, Water Pollutants, Chemical metabolism
- Abstract
In this study, investigation were done on the Mahanadi River water and health of dwelling Indian Knife fish Notopterus notopterus from three sites along the course of the river in an around Cuttack city (Odisha). Oxidative stress biomarker assays such as thiobarbituric acid reactive substances, protein carbonyls, protein and non-protein thionyls, reduced glutathione, metallothionein, superoxide dismutase, catalase, glutathione peroxidase/reductase couple, glutathione-S-transferase, and tissue metal (Fe, Cu, Ni, Cd, Pb and Zn) levels along with water quality assessments were assayed to measure the impacts on fish health. Results indicate that except Fe all other metals studied were within approved limits for fish liver and gill as approved by FAO/WHO. However, the muscle tissue do not have any metal beyond the permissible limit. A site and tissue specific response of the above mentioned oxidative biomarkers as well as metal accumulation in the fish tissues were noticed. Lipid peroxidation and protein carbonylation were increased gradually in the fish tissues collected from experimental sites along the course of the River in comparison to upstream reference site. Glutathione-S-transferase, glutathione peroxidase/reductase couple, reduced glutathione and non-protein thiol content were significantly decreased in fish tissues from experimental sites. An increase in metallothionein content was observed while superoxide dismutase and catalase showed tissue specific responses. Multivariate (Discriminant Function) analysis revealed that lipid peroxidation, protein carbonylation and superoxide dismutase have highest association as predictors of impact in the muscle and liver while that for gill is protein carbonylation, superoxide dismutase and glutathione reductase., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
177. Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility.
- Author
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Samanta L, Swain N, Ayaz A, Venugopal V, and Agarwal A
- Subjects
- Animals, Biomarkers metabolism, Humans, Infertility, Male pathology, Infertility, Male physiopathology, Male, Proteomics methods, Semen Analysis, Signal Transduction, Spermatozoa pathology, Fertility, Infertility, Male metabolism, Protein Processing, Post-Translational, Proteome, Spermatozoa metabolism
- Abstract
Background: The spermatozoa undergo a series of changes in the epididymis to mature after their release from the testis and subsequently in the female reproductive tract after ejaculation to get capacitated and achieve fertilization potential. Despite having a silenced protein synthesis machinery, the dynamic change in protein profile of the spermatozoa is attributed either to acquisition of new proteins via vescicular transport or to several post-translational modifications (PTMs) occurring on the already expressed protein complement., Scope of Review: In this review emphasis is given on the PTMs already reported on the human sperm proteins under normal and pathologic conditions with particular reference to sperm function such as motility and fertilization. An attempt has been made to summarize different protocols and methods used for analysis of PTMs on sperm proteins and the newer trends those were emerging., Major Conclusions: Deciphering the differential occurrence of PTM on protein at ultrastructural level would give us a better insight of structure-function relationship of the particular protein. Protein with multiple PTMs could be used to generate the complex interaction network involved in a physiological function of a sperm. It can be speculated that crosstalk between different PTMs occurring either on same/ other proteins actually regulate the protein stability and activity both in physiological and pathological states., General Significance: The analytical prospective of various PTMs reported in human spermatozoa and their relevance to sperm function particularly in various pathophysiological states, would pave way for development of biomarkers for diagnosis, prognosis and therapeutic intervention of male infertility., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
178. HPV and Cervical Cancer Epidemiology - Current Status of HPV Vaccination in India.
- Author
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Chatterjee S, Chattopadhyay A, Samanta L, and Panigrahi P
- Subjects
- Female, Humans, India epidemiology, Papillomavirus Infections virology, Prevalence, Uterine Cervical Neoplasms immunology, Vaccination methods, Human papillomavirus 16 immunology, Human papillomavirus 18 immunology, Papillomavirus Infections prevention & control, Papillomavirus Vaccines immunology, Uterine Cervical Neoplasms epidemiology, Uterine Cervical Neoplasms virology
- Abstract
Cervical cancer (CaCx) is the second most fatal cancer contributing to 14% of cancers in Indian females, which account for 25.4% and 26.5% of the global burden of CaCx prevalence and mortality, respectively. Persistent infection with high-risk human papilloma virus (HPV- strains 16 and 18) is the most important risk factor for precursors of invasive CaCx. Comprehensive prevention strategies for CaCx should include screening and HPV vaccination. Three screening modalities for CaCx are cytology, visual inspection with acetic acid, and HPV testing. There is no Indian national policy on CaCx prevention, and screening of asymptomatic females against CaCx is practically non-existent. HPV vaccines can make a major breakthrough in the control of CaCx in India which has high disease load and no organized screening program. Despite the Indian Government's effort to introduce HPV vaccination in the National Immunization Program and bring down vaccine cost, challenges to implementing vaccination in India are strong such as: inadequate epidemiological evidence for disease prioritization, duration of vaccine use, parental attitudes, and vaccine acceptance. This paper reviews the current epidemiology of CaCx and HPV in India, and the current status of HPV vaccination in the country. This article stresses the need for more research in the Indian context, to evaluate interventions for CaCx and assess their applicability, success, scalability and sustainability within the constraints of the Indian health care system.
- Published
- 2016
179. Response of testicular antioxidant enzymes to hexachlorocyclohexane is species specific.
- Author
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Samanta L and Chainy GB
- Subjects
- Animals, Body Weight, Lipid Peroxidation drug effects, Male, Mice, Organ Size, Oxidative Stress drug effects, Rats, Rats, Wistar, Species Specificity, Testis pathology, Catalase metabolism, Hexachlorocyclohexane pharmacology, Superoxide Dismutase metabolism, Testis drug effects, Testis enzymology
- Abstract
Aim: To find out whether the response of testicular oxidative stress parameters to hexachlorocyclohexane (HCH) is species specific., Methods: In rats and mice (n=5 in each group), HCH was administered at a dose of 20 mg/kg/day intraperitoneally for 30 days in 0.1 ml of refined groundnut oil. The control groups received equal volume of the vehicle. Animals were sacrificed 24 hours after the last injection and various oxidative stress parameters were measured immediately., Results: The level of both endogenous as well as FeSO4 and ascorbic acid-stimulated lipid peroxidation was increased significantly in the HCH-treated rats, whereas the pattern was just the reverse in case of mice. Although the level of H2O2 content increased in response to HCH in both groups, a totally different trend was observed for the activity of the principal H2O2-metabolising enzyme, catalase. In case of rats, a significant decline in the activity of catalase was recorded in response to HCH whereas a sharp augmentation in the enzyme activity was noticed in mice. Similarly, the decreased activity of superoxide dismutase observed in rats remained unaltered in mice., Conclusion: HCH induces oxidative stress in the testis of both rats and mice. However, the pattern of response of testicular oxidative stress parameters seems to be species specific.
- Published
- 2002
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