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High pre-rigor muscle temperature has negative consequences on quality and has been predominantly studied in the excised longissimus muscle of beef and lamb carcasses. There is little data on other muscles, the application in whole carcasses or potential amelioration techniques such as stretching. This study evaluated the effects of electrical stimulation, high pre-rigor temperature and stretching of lamb sides on quality traits and protein denaturation in four leg muscles [gluteus medius (GM), rectus femoris (RF), semimembranosus (SM) and semitendinosus (ST)]. Twenty lamb carcasses were used with two electrical stimulation treatments (stimulated or non-stimulated, +/−) and two pre-rigor temperature treatments (chilled at 2°C directly after slaughter, or held in 37°C water for 4.5 h before transfer to a 2°C chiller) applied. One side of each carcass was suspended from the Achilles tendon, whereas the other side was stretched by allowing the leg to drop and tying it to the ribs. Electrical stimulation did not influence the different traits except for pH fall post slaughter and myosin denaturation. Stretching resulted in greater muscle and sarcomere lengths for the GM, SM and ST, but a decrease in sarcomere length for the RF. For the non-stretched GM and SM, the 37°C treatment resulted in tougher meat at 1 and 8 days post mortem compared with the 2°C treatment. In contrast, the stretched 37°C treatment resulted in more tender meat for the GM, SM and ST at 1 day post mortem compared with the 2°C treatment. For all muscles, the 37°C treatment resulted in a decrease in the water-holding capacity (increased purge, surface exudate and cooking losses). The magnitude of this effect was generally diminished by stretching for the GM, SM and ST, but for the RF, (which was not stretched by the treatment) this effect was reversed. The 37°C treatment resulted in paler meat (increased L*-values) for the GM, SM and ST relative to the 2°C treatment. The observed effects of the 37°C treatment on water-holding capacity and colour could be explained by the effects of this treatment on indicators of protein denaturation (sarcoplasmic protein solubility and myofibrillar ATPase activity), which were decreased for the GM, SM and ST. The response to both temperature and stretching varied between the muscles, due to different anatomical location and also due to postulated differences in the fibre types. In conclusion, pre-rigor stretching of lamb sides can counteract the negative effects of high early post-mortem temperature on tenderness and water-holding capacity for those muscles that are stretched as a result of this hanging method.

The impacts of accelerated pH decline combined with high muscle temperature on post-mortem muscle metabolism and subsequent meat quality attributes have been extensively studied. Traditionally, this phenomenon has been observed in pork muscles, primarily due to the relatively fast post-mortem glycolysis rate and its relationships to stress susceptibility of pigs before slaughter. However, the protein-denaturing condition of high temperature/rapid pH fall and subsequent PSE (pale, soft and exudative)-like abnormal meat quality characteristics have been observed in muscles from other species such as beef, lamb, venison and even poultry. Various pre-rigor conditions including the application of electrical stimulation, hot-boning, and/or pre-rigor carcass chilling temperatures in various muscles, in conjunction with carcass stretching/hanging methods, can also contribute to muscle-protein denaturation pre-rigor. This review considers the influence of a faster than normal pH fall at a higher than normal pre-rigor temperature on glycolysis, post-mortem muscle proteins and subsequently meat quality attributes. Gaps in current knowledge are identified and recommendations made for additional research.

The core body temperature and post slaughter loin temperatures of steers fed on grass pasture was compared with those of steers fed a grain-based feedlot diet. The feeding treatments were grass for 300 days (Grass), grass for 150 days then feedlot for 150 days (Short Feedlot) and feedlot for 300 days (Long Feedlot). Temperature telemeters were inserted under the peritoneum of the steers and temperature measured at intervals of 1 h for the 300 days, and then at intervals of 1 min for the 48-h period before slaughter. The pH and temperature decline post mortem was also measured. The carcasses of the feedlot steers were heavier and fatter than those from the Grass-fed steers. The core body temperature of the steers from the feedlot treatments was 0.3–0.4°C higher than for the Grass treatment at the time of slaughter. The loin temperature was higher in the feedlot treatments than the Grass treatment at all times measured post mortem as was the temperature at pH 6. Feedlotting can increase the likelihood of ‘high rigor temperature’ conditions of high temperature and low pH occurring in beef carcasses, due to an increase in core body temperature before slaughter, a decrease in the rate of cooling and an increase in the rate of pH decline post mortem. These effects are possibly due to a combination of a direct effect of feed type on body temperature as well as indirect effects on bodyweight and condition score.

Chilled lamb meat exported from Australia has, on occasions, been rejected by importing countries due to greening, after only 6 weeks of storage time. Greening is known to be more prevalent in high ultimate pH (pHu) beef meat (>5.9). There are few data available for lamb carcasses in Australia on the occurrence of high pHu meat, which may have an impact on the understanding and control of quality and greening during storage. The aim of this project was to determine the prevalence of, and influencing factors for, high pHu meat in a range of muscle types in lamb carcasses in Australia. Muscle pHu data were collected from a total of 1614 carcasses from 78 lots at four lamb processing plants in Victoria and New South Wales in autumn and spring of 2013. The pHu of the knuckle (rectus femoris), rack (longissimus) and blade (infraspinatus) was measured and data on carcass and lot characteristics were recorded. Data were subjected to restricted maximum likelihood and generalised linear mixed model analysis. The mean pHu of the knuckle, rack and shoulder were 6.06, 5.79 and 6.12 respectively, and the main factors influencing muscle pHu and occurrence of dark-cutting were breed, season, electrical stimulation and carcass weight. Merino lambs had a higher pHu in the blade and knuckle than did other breeds (P < 0.05, P < 0.01 respectively). Lambs processed in autumn had a higher predicted pHu in the blade and knuckle and a higher percentage dark-cutting (DC; pHu >6.0) for those muscles, than did those processed in spring (P < 0.05). Carcasses that had been electrically stimulated had a higher %DC and a higher pHu in all three muscles (P < 0.05). Carcass weight had a significant effect on the pHu of all three muscles (P < 0.001), with heavier carcasses having a lower pHu and lower %DC. The pHu of the rack was not a reliable predictor for the pHu in other muscles of the lamb carcass. In conclusion, the high occurrence of DC in the muscles, particularly the blade and knuckle, suggests that these muscles may be at risk for producing greening in the vacuum bag during storage.

In the 1990s, the beef and sheep industry in Australia recognised that an eating quality assurance scheme was needed in order to describe and differentiate the eating qualities of different types of beef and sheep meat and to ensure the descriptions were credible (Meat Research Corporation 1996). This was in response to the falling per capita consumption of both beef and sheep meat in Australia (Williams and Droulez 2010) and evidence that the inconsistency in tenderness was causing major problems for both sheep (Safari et al. 2002) and beef (Meat Research Corporation 1996) meat. Furthermore, the recommendations for the proposed grading scheme were that it should encompass whole of chain, from genetics and animal age, through pre- and post-slaughter treatment of the carcass, to the consumer, where cooking technique based on cut and quality were recommended (Meat Research Corporation 1996). The development of the Meat Standards Australia (MSA) grading system, the consumer testing protocols, and the underpinning research for assuring quality to the consumer is summarised for beef by Polkinghorne et al. (2008) and Smith et al. (2008) and for sheep by (Thompson et al. (2005a) and Young et al. (2005).

Dietary betaine supplementation improves water retention in steers and may influence lean-tissue deposition, while also acting as an osmolyte to help regulate cellular osmotic balance. This study investigated the interactions between shade and dietary betaine on carcass characteristics, tissue enzyme activity and gene expression in 48 feedlot steers during summer. Steers were randomly allocated to a 4 × 2 factorial design with the factors being dietary betaine (0, 10, 20 or 40 g) and shade (with and without shade) for 120 days. Tissue samples were obtained at slaughter and analysed for gene expression of heat shock proteins 70 and 90 (HSP70/90) and expression of heat shock factor 1 (HSF1), and enzyme activity of fatty acid synthase (FAS) and glycerol-6-phosphate dehydrogenase (G6PDH). Carcasses were evaluated for quality. Carcass weight at slaughter was not altered by shade (P = 0.18) but tended to be increased by dietary betaine (306 v. 314 kg, P = 0.09). The P8 backfat was not altered by shade (P = 0.43) or dietary betaine (P = 0.32), although there was a within dietary betaine effect whereby P8 backfat tended to be greater in steers fed 10 g compared with 40 g betaine/day (17.4 v. 14.5 mm, P = 0.06). Muscle pH at 1 h (5.97 v. 6.03, P = 0.01) and 2 h (5.73 v. 5.80, P = 0.04) post-slaughter was higher in shaded steers, and muscle pH at 1 h post-slaughter was higher in steers fed 10 or 20 g than those fed 40 g betaine/day (6.03 v. 6.03 v. 5.95, P = 0.005). Gene expression was not altered by betaine, while adipose tissues expressed more of each gene than muscle (P < 0.001). The mRNA expression of HSF1 and HSP90 was influenced by a shade × betaine interaction, although the direction of this interaction was irregular (P = 0.03 and 0.03, respectively). Adipose tissue FAS and G6PDH enzyme activity was unaffected by shade and betaine. The results of this study indicate that betaine supplementation may be a successful carcass modifier in growing feedlot steers during summer. Provision of shade during summer may reduce the rate of pH decline in the first 2 h after slaughter and reduce the risk of high rigor temperature.

Few consumer data are available on the effects of high rigor temperatures on eating quality of different muscles in the beef carcass. The aim of the present study was to investigate the effect of high rigor temperature (heat-toughening) on the consumer and quality traits of two beef muscles. A dataset containing consumer eating-quality scores for 3865 striploins (m. longissimus lumborum) and 734 rumps (gluteus medius) was analysed. Temperature at pH 6 (temp@pH6) was calculated for the striploin and carcasses with a temp@pH6 of >35°C were classified as high rigor temperature (heat-toughened) carcasses. For short ageing periods (1–7 days), high rigor temperature striploins were assessed, by a consumer panel, as being more tender with higher overall liking and higher (more liked) flavour and juiciness, than were striploins entering rigor at a lower temperature. Beyond 14 days of ageing, the high rigor temperature striploins showed minimal improvement in tenderness and the other eating-quality attributes also showed minimal improvements. The consumer scores for tenderness, juiciness, flavour and overall liking for the rump decreased with increasing rigor temperature. High rigor temperature striploins were scored, by trained graders, to have a higher proportion of coarser and softer texture and paler colour. Carcasses defined as ‘high rigor temperature’ will show minimal ageing after extended storage and, at grading, have a higher proportion with pale colour and softer, coarser texture compared to lower rigor temperature carcasses. In conclusion, methods to reduce high rigor temperatures in beef carcasses would improve the acceptability of beef.

The present study investigated the effect of a perennial lucerne and phalaris pasture, or annual pasture with lucerne hay and a range of supplements provided as pellets (700 g/day) on Vitamin E and fatty acid content of skeletal muscle and oxidative stability of lamb meat post-farm gate. Treatments were lambs grazing perennial pasture only (PP); lambs grazing annual pasture with lucerne hay and oat grain pellet supplement (AP); AP with cracked flaxseed (AP+FS); and AP with flaxmeal (AP+FM). After 7 weeks of feeding, lambs were slaughtered after an overnight fast. At 24 h post-slaughter, samples of muscle longissimus lumborum (LL) were collected for determination of fatty acid profile and antioxidant status, as measured by Vitamin E content. Samples were also collected for retail colour assessment of fresh meat at 24 h, 48 h, 72 h and 96 h of post-slaughter. Vitamin E content of the LL from lambs grazing PP was higher (5.9 v. 3.4 mg α-tocopherol/kg, P < 0.01) than that of lambs grazing other treatments. Long-chain n-3 and total n-3 fatty acid contents in the muscle were similar among treatment groups and adequate to claim as a source of n-3. Inclusion of oat grain at 245 g (AP) or at 175 g with flaxseed (AP+FS) or 175 g with flaxmeal (AP+FM) per day in the diet of lambs increased the linoleic acid content (P < 0.05) and the ratio of n-6 : n-3 (P < 0.007) in the LL, compared with lambs grazing PP. Oxidative stability of fresh meat evaluated by retail shelf life (retention of redness) and formation of lipid oxidative substance showed no differences among treatment groups and, as indicated by redness (a*-value) over a 96-h display, all values were within the range (>9.5) for quality meat over the time frame used in the present study. The results provided some evidence that inclusion of PP in the diets of lambs during dry seasons (late summer to autumn) is an effective tool of improving the Vitamin E content of muscle tissues at slaughter.

A major aim of the research program known as SheepGENOMICS was to deliver DNA markers for commercial breeding programs. To that end, a resource flock was established, comprehensively phenotyped and genotyped with DNA markers. The flock of nearly 5000 sheep, born over two consecutive years, was extensively phenotyped, with more than 100 recorded observations being made on most of the animals. This generated more than 460 000 records over 17 months of gathering information on each animal. Here, we describe the experimental design and sample-collection procedures, and provide a summary of the basic measurements taken. Data from this project are being used to identify collections of genome markers for estimating genomic breeding values for new sheep industry traits.

Using performance from progeny born in 2007 and 2008 generated by the Information Nucleus program of the Cooperative Research Centre for Sheep Industry Innovation, preliminary estimates of heritability were obtained for a range of novel carcass and meat attributes of lamb relevant to consumers, including carcass characteristics, meat quality and nutritional value of lamb. Phenotypic and genetic correlations of live animal traits with carcass composition and meat quality traits were also estimated. The data were from progeny located at eight sites, sired by 183 rams from Merino, maternal and terminal meat breeds and were representative of the Merino, Border Leicester × Merino, Terminal × Merino and Terminal × Border Leicester-Merino production types of the Australian sheep industry. Data were available from 7176 lambs for weaning weight, 6771 lambs for ultrasound scanning and 4110 lambs for slaughter traits. For the novel meat quality traits, generally moderate to high heritability estimates were obtained for meat quality measures of shear force (0.27 aged 1 day, 0.38 aged 5 days), intramuscular fat (0.39), retail meat colour (range of 0.09 to 0.44) and myoglobin content (0.22). The nutritional value traits of omega-3 fatty acids and iron and zinc contents tended to have low to moderate heritabilities (0.11–0.37), although these were based on fewer records. Fresh meat colour traits were of low to moderate heritability (0.06–0.21) whereas measures of meat pH were of low heritability (~0.10). For the carcass traits, estimates of heritability were moderate to high for the various measures of carcass fat (0.18–0.50), muscle weight (0.22–0.35), meat yield (0.24–0.35), carcass muscle dimensions (0.25–0.34) and bone weight (0.27). Results indicate that for most lamb carcass and meat quality traits there is sufficient genetic variation for selection to alter successfully these characteristics. Additionally, most genetic correlations of live animal assessments of bodyweight, muscle and subcutaneous fat with the carcass and meat quality traits were favourable. Appropriate definition of breeding objectives and design of selection indexes should be able to account for the small unfavourable relationships that exist and achieve the desired outcomes from breeding programs.

Eruption of permanent incisors (dentition) is used as a proxy for age for defining meat quality in Australian sheep meat. However, this approach may not be reliable. While not presently available, an objective method could be used to determine sheep age, and thus sheep category, which would then potentially remove any inaccuracies that may occur in classifying sheep meat product. Statistical classification algorithms have been successfully used in bioinformatics. In this paper we review the performance of three algorithms (support vector machines, recursive partitioning and random forests) for determining sheep age. The algorithms were applied to the measured fatty acid profiles of fat samples from 533 carcasses; 254 lamb (2 years old) samples. Three data pretreatments (range transformation, column mean centering and range transformation with mean centering) were also examined to determine their impact on the performance of the algorithms. The random forests algorithm, when applied to mean-centred data, gave 100% predictive accuracy when classifying sheep category. This approach could be used for the development of an objective test for determining sheep age and category.

The tenderness of 160 samples of m. longissimus thoracis et lumborum (LL) from 40 sheep carcasses was measured at two laboratories, 80 samples per laboratory, with two ageing times (0 and 5 days). The samples were all cooked from frozen and then measured on the same type of machine, a Lloyd Texture analyser with a Warner–Bratzler-type shearing blade attached to give a shear force value for each sample. Shear force results were natural log-transformed and the analysis showed that there was a significant (P < 0.001) effect of ageing on shear force and a significant (P = 0.01) difference between the laboratories. Thus, on equivalent samples, Laboratory B produced shear force results 0.78 times those for Laboratory A. There was no significant (P > 0.05) effect of ageing on cooking loss, but there was a significant (P < 0.05) difference between the laboratories. On equivalent samples, Laboratory B produced lower cooking loss results (expressed as a percentage), on average by 3.1%, than did Laboratory A. When cooking loss was included in the model for shear force, it was significant (P < 0.001), and its inclusion reduced the significance (P = 0.04) of the difference between laboratories. Thus, part of the differences between the two laboratories for shear force could be explained by the difference in the cooking loss at the two laboratories. As cooking loss increased, shear force increased.

The efficacies of electrical stimulation practices at Australian abattoirs that process significant proportions of Australian lamb and sheep meat were assessed. Several abattoirs used medium-voltage electrical stimulation system (MVS) units suboptimally and many carcasses at these abattoirs exhibited a poor rate of pH decline and, consequently, a low proportion of carcasses attained pH 6 at a carcass temperature of 18−35°C, according to Sheep Meat Eating Quality pH–temperature guidelines as adopted by Meat Standards Australia. In the present communication, we describe the causes of suboptimal results with electrical stimulation and discuss procedures for optimising the stimulation dose delivered by MVS units. Carcass pH responses to various levels of current and pulse width were assessed to determine the best MVS settings for individual abattoirs. The MVS units at the abattoirs involved in the present study have now been programmed for optimal efficacy and carcass quality will be monitored on an ongoing basis. Evaluation of methods for assessing the proportion of carcasses that fall within the optimum pH–temperature window showed that a random regression model is more accurate than the current approach.

Thirty 7-month-old crossbred lambs (Poll Dorset × Border Leicester × Merino) finished over 5 weeks on either low quality pasture or grain-with-hay on a farm in southern Victoria were assessed for carcass parameters, muscle fat composition and retail colour stability. Lambs on the grain diet had a mix of barley grain (80%) and lentils (20%) at 800 g/head.day (air-dry basis ~ad libitum) with cape weed (Arctotheca calendula) hay available at all times. Lambs under grazing had predominantly rye grass (Lolium perenne) and barley grass (Hordeum leporinum) available ad libitum. Carcass weight tended to be higher (P = 0.14) in grain-fed lambs than in grass-fed lambs, but fatness indicated by GR (total muscle + fat tissue thickness at 11 cm from midline) did not differ between feeding systems. Fatty acid composition was determined in the loin from the forequarter (M. longissimus thoracis) and lumbar (M. longissimus lumborum) regions and from the leg region (M. semimembranosus). This showed that grain-finished lamb had higher muscle fat (P < 0.001) and omega-6 fatty acid (P < 0.001) content. Alpha-linolenic acid, eicosapentaenoic acid (EPA) or total omega-3 fat did not differ (P > 0.05) between feeding groups. Saturated fatty acids were greater (P < 0.01) in both loin sampling sites than the leg. The levels of EPA, docosahexaenoic acid and docosapentaenoic acid were lower (P < 0.01) in the forequarter (9%) or lumbar (11%) sites than the leg sampling site. The distribution pattern of fatty acids across the three sampling sites did not differ between feed types. Retail colour stability determined over 4 days of display (only performed in muscle from the lumbar site), evaluated by the redness (HunterLab a*-value) and metmyoglobin formation (reflectance ratio at 630 : 580-nm wavelengths) was superior for grass-fed lamb compared with short-term grain-finished lamb. The results demonstrate that the health claimable omega-3 fat mainly EPA or total omega-3 fat content in lamb was not altered by short-term grain finishing compared with lamb finished under pasture grazing conditions.

The effects of production and processing factors on tenderness, and colour of lamb meat produced from 7 locations as part of the Australian Sheep Industry CRC’s Information Nucleus flock were investigated, using data from 2052 lambs slaughtered in 2007. At 24 h post-slaughter, samples of m. longissimus lumborum (LL) and m. semimembranosus (SM) were collected for measurement of intramuscular fat (IMF), myoglobin, iron and copper and fresh meat colour (L*, a*, b*) and pH at 24 h measured on the LL. pH and temperature measurements made pre-rigor were used to calculate the pH at 18°C. Tenderness was measured by LL shear force at days 1 (SF1) and 5 (SF5) post-slaughter, the shear force difference (SF-diff) and SM compression and collagen concentration were determined. Retail colour stability was assessed using over-wrapped LL under simulated retail display for 3 days, according to the change in the oxymyoglobin/metmyoglobin ratio. All traits were affected by flock and date of slaughter (P < 0.001). After 4 days of ageing, 70–95% of the LL samples from all flocks, except for one, had acceptable tenderness for consumers based on their shear force. Low IMF, high LL pH at 18°C and high pH at 24 h increased SF1 and SF5 and also had an effect on SF-diff (P < 0.001). The retail colour of 44.8% of the samples on day 3 of retail display were lower than acceptable. Retail colour was influenced by IMF, pH18 and the concentration of iron and copper (P < 0.001). In conclusion, breeding and management practices that increase muscle IMF levels and reduce ultimate pH values and processing practices that result in moderate rates of pH fall post-slaughter, improve the tenderness of lamb. Extension of retail colour stability may be antagonistic to traits associated with tenderness and nutritional traits, particularly IMF and mineral levels.

Quality attributes of the longissimus lumborum (LL) were compared to the attributes of the muscles semimembranosus (SM), rectus femoris (RF), biceps femoris (BF), gluteus medius (GM) and the lateral portion of the semitendinosus (ST) in the ham, the psoas major (PM) in the loin and the supraspinatus (SS), infraspinatus (IS) and triceps brachii (TB) in the shoulder. Carcasses were allocated to quality groups based on measurements of muscle exudate and lightness (CIE-L(∗)) in the LL. The SM, BF, GM and ST in the ham were similar to the LL (P > 0·05) in meat ultimate pH (pHu) and exudate and the GM, ST and LL were similar (P > 0·05) to the LL in lightness. The TB, RF, SS, PM and IS were similar (P > 0·05) in exudate, lightness and pHu and compared to the LL, were less exudative (P < 0·05) and darker (P < 0·05). When the LL was classed pale, and exudative, the GM, BF, SM and ST were also pale and exudative but the other muscles were darker and non-exudative. When the LL was classed dark and non-exudative, all other muscles were dark, non-exudative and the pHu was high. Therefore, the LL could serve as a reliable indicator of colour and exudate for all porcine musculature when the condition was dark and non-exudative (i.e. DFD). However, when the condition was pale and exudative (i.e. PSE), then the LL would serve as a reliable indicator only for the four major ham muscles, excluding the RF.

Lean meat and fat content of Australian sheep genotypes were compared at 20-, 30- and 40-kg carcass weights. Sheep comprised Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Border Leicester × Merino (BL × M) and Merino × Merino (M × M) genotypes. Lambs were raised as a mixed flock under grazing and slaughtered at 4, 8, 14 and 22 months of age with each slaughter time involving ~150 mixed sex animals. At 24 h after slaughter, chilled carcasses were halved along the backbone and the right sides were used for determination of lean, fat and ash percentages using dual energy X-ray absorptiometry. Within a particular age group and genotype, animals growing at faster rates and reaching heavier carcass weights had lower carcass lean meat content than slower growing animals. Merino carcasses weighing 20 and 30 kg had similar levels of lean meat to PD × M genotypes, which was greater than that from the BL × M genotype. Second-cross PD × BLM carcasses weighing 20 kg at 4 months and 30 kg at 8 months had similar carcass fat and lean percentages to 20-kg Merino carcasses at 8 months and first-cross PD × M carcasses weighing 30 kg at 14 months, respectively. At 40-kg carcass weight, 22-month-old Merinos had similar levels of leanness to carcasses from 22-month-old PD × M animals and carcasses from 14-month-old second-cross PD × BLM animals. Carcass lean meat content decreased with increasing carcass weight and first- cross BL × M animals had the lowest carcass lean across all weight categories. There was a major acceleration in carcass fatness between 14 and 22 months associated with a reduction in muscle deposition. Results indicate that age of the animal should be taken into account when carcass lean and fat contents are compared at a particular carcass weight. Merinos will achieve weight/composition specifications at least equally well to crossbreds but will take longer with a likely increase in production costs.

Adrenergic activation and hormone release preslaughter is an inevitable outcome of the systems used to move cattle to slaughter. The aim of this experiment was to investigate the effects of acute preslaughter stress in beef cattle on postmortem muscle metabolism and the meat quality, including consumer-assessed eating quality. Eighty-four cattle were used on three separate days, with ‘mobs’ of four cattle allocated to either a ‘control’ (no electric goads used preslaughter) or a ‘stress’ (six prods given with an electric goad over 5–10 min) treatment at 15 min preslaughter. Cattle undergoing the ‘stress’ treatment had higher plasma lactate at slaughter. The prerigor pH and temperature, ultimate pH and temperature at rigor of the longissimus thoracis muscle were similar between treatments (P > 0.05 for all). The water-holding capacity of the longissimus lumborum was reduced by the ‘stress’ treatment, as indicated by higher levels of water lost during suspension (drip loss), storage (purge) for 21 days and cooking (cooking loss at 1 day postslaughter) (P 0.05 for all). In conclusion, cattle subjected to acute preslaughter stress using electric goads produced meat which the consumer rated as tougher with inferior quality. The inferior quality induced by the acute stress treatment was associated with reduced water-holding capacity but was independent of muscle pH and temperature.

This study was conducted to determine the effect of direct consignment compared with saleyard marketing on beef quality and palatability. A total of 258 cattle (mean carcass weight 227 ± 19 kg) from nine vendor properties in Victoria, Australia were used. From each vendor group (about 30 cattle/vendor), half were either: (1) processed through a saleyard and then sent to the abattoir or (2) directly consigned to the abattoir. All cattle were slaughtered at the same abattoir and the lairage and postslaughter management of the cattle and their carcasses was standardised. The cattle that had been directly consigned were slaughtered the day after dispatch from the property, whereas saleyard cattle were slaughtered 2 days after dispatch. Striploin (longissimus lumborum) samples were evaluated 1 day postslaughter and after 14 days aging. Overall, marketing method had only a small impact on the various meat quality measures and palatability. A significant vendor × marketing method interaction was found for most traits including muscle glycogen (semimembranosus and semitendinosus), pH (1, 3 and 24 h postslaughter), L*, a* and b* colour values and consumer panel scores [tenderness, flavour and combined score (MQ4)]. Juiciness scores were unaffected by marketing method but were significantly influenced by vendor group (P < 0.001). For MQ4 score, there was a general trend showing that steaks from cattle that had been marketed through the saleyard had marginally lower MQ4 scores than those that had been directly consigned in five of the eight groups. However, this trend was only significant for two of the five groups. A significant three-way interaction between vendor group × marketing method × aging duration was found for shear force (P < 0.001) and cooking loss percentage (P < 0.001). The effect of marketing method on shear force was generally small and not always statistically significant but there was a trend indicating that saleyard marketing resulted in slightly higher shear forces at either 1 or 14 days postslaughter for the majority of the vendor groups. It was concluded that marketing method had a small but variable impact on palatability and meat quality.

This study compares the carcass composition of different genotypes of Australian sheep slaughtered at four ages (110, 236, 412 and 662 days of age, or 4, 8, 14 and 22 months of age). The genotypes used were Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Merino × Merino (M × M) and Border Leicester × Merino (BL × M). Approximately 150 mixed sex animals were slaughtered at each age and the right sides (half carcass) were scanned by dual energy X-ray absorptiometry (DXA) for chemical lean, fat and bone mineral percentages of the carcass. Carcass lean percentage decreased (P < 0.001) across sire groups from Merino to Poll Dorset to Border Leicester at each age, while fatness increased (P < 0.001) in the opposite direction. With age, the magnitude of difference between genotypes for fatness became greater. There was a marked increase (P < 0.01) in ash mineral percentages in M × M and PDg × M genotypes relative to other genotypes in the 14- and 22-month groups. There were no differences (P > 0.1) between PDg × M and PDm × M in either chemical lean or fat percentages at any age but ash mineral percentage was higher in the PDg × M genotype at older ages (P < 0.05). This in turn led to differences in chemical lean : ash mineral content at later ages between PDg × M and PDm × M. Wethers had higher chemical lean (P < 0.05) and lower fat (P < 0.05) percentages in the carcass than ewes at all ages but ash mineral and chemical lean : ash mineral content did not differ between sexes. It was clear that the level of chemical lean or fat or ash mineral composition reached by some genotypes at one age was equivalent to that reached by other genotypes at the next measurement age. Those genotypes that reach a specification at younger ages will be more productive for meat production. Purebred Merino genetics will always be less productive in terms of carcass weight and muscle related productive traits (loin weight, semitendinosus muscle weight, eye muscle area) than BL and PD genetics, but the relative productivity of PD and BL genetics will depend on the traits used in the specification [see also Ponnampalam EN, Hopkins DL, Butler KL, Dunshea FR, Warner RD (2007) Genotype and age effects on sheep meat production. 2. Carcass quality traits. Australian Journal of Experimental Agriculure 47, 1147–1154]. The results suggest that selection for muscling had a greater impact on reducing ash mineral content associated with bone mineral mass than on increasing lean mass in the carcass.

A Hologic QDR4500W dual energy X-ray absorptiometer (DXA) was used to measure body composition in 60 sheep half carcasses ranging from 8 to 28 kg. Half carcasses were from ewes and wethers of mixed genetics. Values determined by DXA, including total tissue mass (TTM), lean tissue mass (LTM), fat tissue mass (FTM) and bone mineral content (BMC), for the half carcass were evaluated by comparison with chemically determined composition. In the case of BMC, the relationship was with chemically determined ash content. Liveweight and chemically determined lean, fat and ash were strongly related to DXA-derived values for TTM, LTM, FTM and BMC, respectively (R2 = 0.999, 0.986, 0.989 and 0.920, respectively). However, because DXA estimates were different from chemically determined values in this sample of carcasses, they needed to be adjusted with the use of appropriate regression equations to correct the in-built algorithms. These data demonstrate the efficacy of DXA as a non-destructive method for determining the composition of the sheep half carcass.

The present study examines carcass quality traits in different genotypes of Australian sheep slaughtered at 4, 8, 14 and 22 months of age. Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Merino × Merino (M × M) and Border Leicester × Merino (BL × M) genotypes were compared. Sires were selected on the basis of growth (PDg, M and BL) and muscle (PDm) using estimated breeding values (EBVs). Approximately 150 mixed sex animals were slaughtered at each age (595 in total for the four slaughters) and carcass quality traits, including hot carcass weight, loin and semitendinosus (ST) muscle weights, GR fat, fat C, rump fat depth and eye muscle area (EMA), were determined at 45 min to 3 days postslaughter. With age, all carcass quality traits increased in magnitude. Merinos produced the lightest and leanest carcasses (P < 0.01) with lowest values in nearly all the carcass quality traits. At 4 and 8 months of age carcass and loin weights, EMA and GR fat measures were greater (P < 0.01) in second-cross PDg × BLM sheep than all other genotypes. However, traits related to muscle content were similar between first- and second-cross Poll Dorset genotypes at 14 and 22 months of age. The BL × M genotype had lower values than the PD × M genotypes for these traits, at all ages. Traits related to carcass fatness showed that sheep with Border Leicester genetics tend to deposit more fat (P < 0.001) in the carcass than those with Merino or Poll Dorset genetics from 14 months of age onwards. Most carcass quality traits did not differ (P > 0.05) between first-cross Poll Dorset genotypes selected for growth or muscling (PDg × M v. PDm × M). Sires with high muscling EBVs are associated more with shorter carcass length than low muscling EBVs. From ~8 months of age, wethers had greater values (P < 0.05) than ewes of the same age for most carcass quality traits related to size and muscle development, but no effect was present for carcass quality traits that were related to fat deposition. It was clear that the carcass weight of M × M at 8 and 14 months were similar to that observed in PDg × BLM at 4 and 8 months, respectively. Loin weights of M × M and BL × M at 13 and 8 months were similar to that weights observed in PDg × BLM at 8 and 4 months of age, respectively. Animals slaughtered to a muscle weight specification will reach the target at a younger age when they have more Poll Dorset than Border Leicester background genetics. Alternatively animals slaughtered at a specified fat score (using fat C or rump fat depth) will reach the specification at a younger age when they have more Border Leicester than Poll Dorset genetics.

The combined effects of age and genetics and Poll Dorset sire and growth path were studied in two separate experiments (n = 595 and 627, respectively). In the first experiment, containing genotype crosses typically used in Australia (Merino, Poll Dorset, Border Leicester) and sires selected for growth or muscling, sheep were slaughtered at 4, 8, 14 and 22 months. The second experiment used Poll Dorset sires selected for high muscling, fat or growth with progeny having two levels of nutrition postweaning. Border Leicesters expressed higher levels of carcass fat percentage and intramuscular fat and produced the heaviest carcass. Merinos had the lowest subcutaneous fat depth and highest carcass lean percentage when compared at the same age. The progeny of Poll Dorset sires selected for high muscling (PDm) expressed a shift toward glycolytic fibres relative to those from Merino sires, and PDm sires produced progeny with reduced spine and limb length and higher carcass muscle : mineral ratios, suggesting skeletal stunting. Genotype meat quality differences were minimal except that PDm sire topsides were tougher and Merinos produced higher pH meat. With age (4–22 months), lambs became heavier and fatter, fibres shifted towards oxidative and away from glycolytic, muscle myoglobin increased, the meat became darker and redder and tenderness declined. Early weaning had no effect on the time to reach slaughter weight, provided nutrition was not restricted. The sire genetics influence on the carcass composition far outweighed the effect of nutrition postweaning. Lambs on a restricted diet tended to have less acceptable meat quality but this was not evident in lambs from sires selected for high fatness. Sensory tenderness was improved and intramuscular fat was higher in lamb progeny from sires selected for high fatness.

This paper provides an introduction to this Special Issue of the Australian Journal of Experimental Agriculture. The special issue is dedicated to an integrated research program that was intended to increase the understanding of the effects of breed and genetic selection for live animal traits (growth, muscle and fatness) on carcass and meat attributes. Combined with this are the effects of animal age and nutritional restriction at critical weaning points. The reasons for, and general approach, of the research are outlined.

The aim of this experiment was to compare the effects of electrical stimulation and lamb age on the retail colour stability of lamb meat. Poll Dorset Merino crossbred lambs that were 5 months (‘suckers’, SU) and 12 months (‘carryovers’, CO) finished on the same green annual pasture were slaughtered at a commercial abattoir. Half of the carcasses in each age group (10) were either electrically stimulated (ES) or not stimulated (NES) post-dressing with a commercial high voltage electrical stimulation system. Luminescence (L), hue angle (indication of redness), chroma (intensity), and oxy/met (a measure of browning) values were measured at 0 h (0 days), 21 h (0.86 days), 93 h (3.88 days), and 166 h (6.91 days) after meat had been cut 1 day after slaughter, over wrapped, and put under simulated retail display conditions. Three muscles, m. longissimus thoracis et lumborum (LL), m. gluteus medias (GM), and m. rectus femoris (RF), were measured in this way. A linear mixed model was used to fit a repeated-measurements model to the light measurements of L, hue angle, chroma, and oxy/met. Oxy/met rate of change was compared by fitting splines to predict the time required for oxy/met to reach an arbitrary benchmark value of 3. Colour and rate of colour change during display were affected by both age class and electrical stimulation but these effects depended on the muscle. The LL was the darkest (lowest L) and reddest (lowest hue angle), whilst RF was the most stable in colour (lowest rate of change for oxy/met) of the 3 muscles tested. Age class had a greater effect on oxy/met rate of change for the LL and GM compared with RF. Oxy/met of LL changed more rapidly for CO than SU lambs. ES increased the time for oxy/met to reach 3 in RF and GM for SU only but did so for both age groups in LL. ES decreased this time in RF and GM for CO. CO meat contained a higher concentration of myoglobin than SU meat. Negative effects of electrical stimulation on colour stability are more likely to occur in older lambs and in the GM and RF rather than in the LL.

Dark-cutting in muscles of the beef carcass is due to low muscle glycogen levels at slaughter and occurs particularly in autumn and winter in grass-fed cattle in southern Australia. The aim of these experiments was to investigate the effect of supplementary feeding of cattle grazing pasture during winter on muscle glycogen levels. The first experiment involved 70 cattle allocated to two stocking rates grazing improved perennial ryegrass and subterranean clover pastures [high stocking rate (HSR) v. low stocking rate (LSR)] by two pasture feeding regimes (control, pasture only v. pasture supplemented with a high-energy ration for 4 weeks) plus a feedlot treatment (fed high-energy ration in pens with no pasture for 11 weeks). Muscle biopsies were collected from the M. semitendinosus (ST) and M. semimembranosus (SM) muscles and analysed for muscle glycogen. The ST muscle glycogen content for supplemented animals increased (P < 0.05) over the feeding period but there was no effect (P > 0.05) of supplementation on the muscle glycogen content of the SM or on the muscle glycogen content of the ST or SM of cattle in the feedlot treatment, relative to control cattle. HSR cattle tended to have lower muscle glycogen in the ST compared to LSR cattle across both feeding regimes. The second experiment used 60 cattle allocated to two treatments (control, pasture only v. pasture supplemented with a high-energy ration for 3 weeks). The treatments were applied to cattle grazing improved perennial ryegrass and subterranean clover pastures and muscle biopsies were collected weekly from the SM and ST. Supplementation resulted in a linear increase (P < 0.05) in muscle glycogen levels over the 3 weeks in both the SM and ST muscles. These results indicate that feed quality has a major impact on muscle glycogen levels in the SM and ST of cattle destined for slaughter. At times of the year when pasture quality is poor or quantity is lacking, supplementation with a high-energy supplement has the potential to dramatically increase muscle glycogen and reduce the incidence of dark-cutting beef.

Combined actions of nutrition and genetic regulation of the growth rate of lambs as well as the physical, biochemical, and eating quality characteristics of their skeletal muscle were assessed in a major field experiment. Data arising were collated and integrated to consolidate findings made at the farm, animal, tissue, cellular, and gene expression levels. At the farm level, increased nutrient availability significantly increased the growth rate of crossbred lambs and increased the growth advantage resulting from the use of sires with high estimated breeding values (EBV) for growth. In contrast, the extra depth of the M. longissimus thoracis et lumborum (EMD) arising from sires with a higher EBV for this trait was constant irrespective of nutrition. Ewe liveweight and body condition were critical in determining the pre-weaning nutrition and growth of lambs, with the LOW plane of nutrition causing stunting of forelimb bones and changes to the allometric growth coefficients for carcass lean and fat. The EBV of the sire for muscling (PEMD) influenced several non-muscling traits, and interactions with nutrition suggested that on HIGH nutrition, absorbed nutrients were partitioned away from wool and fat accretion and towards protein accretion in lambs having superior muscling genetics. Expression levels of known myogenic factors in muscle, together with a suite of peptides and proteins whose identity and levels were determined by proteomic screening, contributed to improved understanding of the mechanisms underpinning nutritional and genetic regulation of skeletal muscle development. The study revealed the need for caution in the use of indirect markers of growth or composition, with their usefulness being constrained if the localisation of the response to selection is specific to parts of the carcass where the selection had initially been concentrated. As well, the possibility that much of the variation explained by potential physiological markers can be accounted for by non-invasive measures of growth and fatness currently used by Australian farmers must be considered.

This paper provides an introduction to the special edition of the Australian Journal of Agricultural Research dedicated to an integrated research program to understand the effects of genetic selection for live animal traits (growth, muscle, and fatness) that determine carcass and meat attributes. Combined with this are the effects of nutritional status of the dam and growing lamb. The reasons for, and general approach of, the research is outlined.

One constraint facing the pig industry is that ad libitum feeding can often result in high levels of body fat and technologies which can reduce the ratio of lean to fat deposition in the pig are continually being explored. Conjugated linoleic acids have been shown to decrease body fat content in pigs. Therefore, the aim of this study was to determine whether dietary conjugated linoleic acids supplementation has any effect on meat quality and carcass characteristics in finisher pigs. Sixty female crossbred (Large White × Landrace) pigs (average initial weight 56.6 ± 1.9 kg and average initial P2 backfat 11.4 ± 1.3 mm) were used in the present study. Pigs were individually housed and randomly allocated to 1 of 6 dietary treatments: 0, 0.125, 0.25, 0.50, 0.75 or 1.0% (w/w) of conjugated linoleic acids-55. The wheat-based diets were formulated to contain 14.3 MJ DE and 9.3 g available lysine per kg and were fed ad libitum for 8 weeks. Pigs were slaughtered and meat quality was determined on the longissimus thoracis using standard techniques. Dietary conjugated linoleic acids reduced subcutaneous back fat in a linear manner with effects being most pronounced in the middle back fat layer. There was also a linear (P

The effects of acute exercise stress and electrical stimulation on lamb eating quality and objective measurements of quality were evaluated on the Longissimus thoracis et lumborum (LTL) and the Gluteus medius (GM) muscles. Meat quality attributes were also assessed on the LTL, Semimembranosus (SM) and the Semispinalis capitis (SC) muscles. Forty-eight Poll Dorset × Border Leicester–Merino ewes of about 6 months old were randomly allocated to treatments in a 2 × 2 factorial design, with the respective factors being exercise (Ex, 0 v. 15 min) and post-slaughter low voltage electrical stimulation (control v. ES). In general, exercise reduced the rate of pH fall and increased the ultimate pH of all 3 muscles (P

Dark-cutting in beef carcasses is a quality and economic problem for the grass-fed beef industry in Australia, with ~10% of carcasses graded as dark-cutting. Dark-cutting results from low muscle glycogen levels at the time of slaughter. An experiment was designed to examine the relationship between season and muscle glycogen levels for cattle at pasture. Sixty steers were allocated to 2 stocking rate treatments, low and high (1.5 and 2.5 steers/ha, respectively) with 3 replicates for each treatment and grazed in 6 separate paddocks. Monthly samples of the M. semimembranosus (SM) and M. semitendinosus (ST) were taken by biopsy from all cattle and analysed for glycogen and lactate content. Significant differences in muscle glycogen were found between seasons. Average muscle glycogen levels for autumn, winter, spring and summer were 1.24, 1.00, 1.15 and 0.82 mg/g SM and 0.85, 0.91, 1.05 and 0.76 mg/g ST, respectively. The seasonal effects on muscle glycogen were not influenced by stocking rate, but it is postulated that they were influenced by nutrition, with the peak in muscle glycogen level generally coinciding with the peak in pasture quantity and quality in spring.

Ninety crossbred (Large White × Landrace) female pigs were used to compare the effects on ultimate pork quality of different magnesium (Mg) compounds [magnesium aspartate (MgAsp) and magnesium sulfate (MgSO4)], Mg dose (1.6 and 3.2 g elemental Mg), and Mg supplementation period (2 and 5 days) in pigs subjected to an acute stressor pre-slaughter. Pigs fed the Mg-supplemented diets had lower muscle lactic acid concentrations at 24 h post-slaughter than pigs fed the control diet. There were no significant differences in muscle glycogen concentrations in the Longissimus thoracis (LT) at 5 min, 40 min, or 24 h post-slaughter between the control and Mg-supplemented diets. Pigs fed the Mg-supplemented diets produced less pale colour pork (i.e. darker colour) and had reduced drip loss and reduced incidence of pale, soft, exudative (PSE) pork in the LT muscle compared with pigs fed the control diet. Within the Mg diets, pigs fed the MgAsp-supplemented diet produced less pale LT muscle colour than pigs fed the MgSO4-supplemented diet. There were no significant differences in LT pork quality parameters (colour and percent drip loss) regardless of whether the high or low Mg dose or the 2- or 5-day supplementation period was used. This experiment has shown that the use of dietary MgAsp supplementation at a dose of 1.6 g elemental Mg (20 g MgAsp) for 2 days prior to slaughter significantly improved pork quality and reduced the incidence of PSE pork.

Forty-eight crossbred (Large White × Landrace) boars were used in a 2 × 2 factorial design to determine the effects of mixing unfamiliar pigs during lairage and the use of electric goads just prior to slaughter on muscle glycogenolysis and subsequent pork quality. The treatments included 2 mixing treatments (familiar pigs kept together during lairage and unfamiliar pigs mixed during lairage) and 2 handling treatments [minimal handling and negative (electric goad) handling pre-slaughter]. Pigs mixed during lairage had lower muscle glycogen and lactic acid at 5 min, 40 min, and 24 h post-slaughter than familiar pigs kept together during lairage. Mixing of unfamiliar boars also resulted in higher ultimate muscle pH, a higher incidence of dark, firm, dry (DFD) pork, and a lower incidence of pale, soft, exudative (PSE) pork compared with familiar pigs kept together during lairage. The use of an electric goad prior to slaughter did not affect muscle glycogen in mixed pigs, but in unmixed pigs there were large numerical diffferences in glycogen concentrations. The negatively handled unmixed pigs had a trend for lower glycogen concentrations which did not reach significance. This was probably due to the small sample size. However, pigs negatively handled pre-slaughter had higher lactic acid concentration at 5 min post-slaughter compared with minimally handled pigs. This experiment has demonstrated that mixing of unfamiliar boars can have a major impact on pork quality, resulting in a high incidence of DFD pork and a reduced incidence of PSE.

Thirty-six crossbred (Large White × Landrace) boars were used to investigate the effect of positive and negative handling of pigs on-farm over a period of 5 weeks, and the effect of minimal and negative handling just prior to slaughter at the abattoir on the level of stress and meat quality of the carcass. There was no significant difference in adrenal gland weight and plasma cortisol concentration measured at slaughter between pigs negatively and positively handled on-farm. Pigs negatively handled on-farm had lower muscle glycogen 5 min and 40 min post slaughter in the Longissimus thoracis (LT) but muscle paleness and percentage drip loss of the LT were similar compared with pigs positively handled on-farm. Pigs negatively handled at the abattoir had lower muscle glycogen 5 min post slaughter in the LT; however, muscle paleness, percentage drip loss, ultimate pH, and incidence of pale, soft, exudative meat were similar compared with pigs that were minimally handled at the abattoir. This study has shown how the interaction between pigs and humans on-farm can influence the pig’s ‘stress’ response to pre-slaughter handling and, potentially, meat quality.