151. Serum biomarkers that predict lean mass loss over bed rest in older adults: An exploratory study
- Author
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Robert R. Wolfe, Susan H. Gawel, Menghua Luo, Gerard J. Davis, Suzette L. Pereira, and Nicolaas E. P. Deutz
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Clinical Biochemistry ,Bed rest ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Serum biomarkers ,Internal medicine ,Medicine ,Humans ,Functional decline ,Muscle, Skeletal ,Rest (music) ,Aged ,business.industry ,Biochemistry (medical) ,Healthy subjects ,General Medicine ,Middle Aged ,Serum samples ,030104 developmental biology ,Blood biomarkers ,030220 oncology & carcinogenesis ,Cardiology ,Lean body mass ,Body Composition ,Female ,business ,Bed Rest ,Biomarkers - Abstract
Background Lean mass (LM) loss during extended bed rest contributes to long term functional decline in older adults. Identifying blood biomarkers that predict a hospitalized individual’s risk of losing LM could allow for timely intervention. Methods LM from 19 healthy subjects (age 60–76 y, 4 males, 15 females), who were confined to 10 days of complete bed rest, was measured pre- and post-bed rest. One hundred eighty-seven biomarkers from pre-bed rest fasted serum samples were obtained from all evaluable subjects (n = 18), analyzed using multiplexed immunoassay array and pooled. Decision tree analysis was used to identify pre-bed rest markers that predict LM loss over bed rest. Results Sixty-three markers were excluded due to being below assay detection limits. One pair of markers, Tissue inhibitor of metalloprotease-1 (TIMP1) and tenascin C (TNC), were found to correlate with percent change in total LM over bed rest: [R2 = 0.71, all subjects; R2 = 0.76, females]. Subjects with pre-bed rest TIMP1 ≥ 141 ng/ml had the highest loss of total LM over bed rest, whereas subjects with pre-bed rest TIMP1 Conclusions Panels of blood biomarkers associated with the muscle extracellular matrix may predict the likelihood for LM loss over extended bed rest.
- Published
- 2020