466 results on '"Qiqi Li"'
Search Results
152. Bioaccumulation of emerging persistent organic pollutants in the deep-sea cold seep ecosystems: Evidence from chlorinated paraffin
- Author
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Lina Lyu, Kejing Fang, Zhenchang Zhu, Jie Li, Yu Chen, Lin Wang, Zhimao Mai, Qiqi Li, and Si Zhang
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Environmental Engineering ,Health, Toxicology and Mutagenesis ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Abstract
Persistent organic pollutants (POPs) are highly toxic and can accumulate in marine organisms, causing nonnegligible harm to the global marine ecosystem. The Cold seep is an essential marine ecosystem with the critical ecological function of maintaining the deep-sea carbon cycle and buffering global climate change. However, the environmental impact of emerging POPs in the deep-sea cold seep ecosystem is unknown. Here, we investigated the potential pollution of chlorinated paraffins (CPs) and their bioaccumulation in the cold seep ecosystem. High concentrations of CPs were detected in the cold seep ecosystems, where CPs bioaccumulated by the keystone species of deep-sea mussels can be released into the surface sediment and vertically migrate into the deeper sediment. Furthermore, more toxic CPs were accumulated from transforming other CPs in the cold seep ecosystem. Our study provides the first evidence that high concentrations of POPs are bioaccumulated by deep-sea mussels in the cold seep ecosystem, causing adverse ecological effects. The discovery of CPs bioaccumulation in the deep-sea cold seep ecosystem is a crucial mechanism affecting deep-sea carbon transport and cycling. This study has important guiding significance for revealing the deep-sea carbon cycle process, addressing global climate change, and making deep-sea ecological and environmental protection policies.
- Published
- 2023
153. Analysis of accumulation models of Middle Permian in Northwest Sichuan Basin
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Qingong Zhuo, Bin Li, Mei Wenhua, Xuesong Lu, and Qiqi Li
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Maturity (geology) ,geography ,geography.geographical_feature_category ,Source rock ,Permian ,Geochemistry ,Anticline ,General Earth and Planetary Sciences ,Thrust fault ,Fault (geology) ,Foreland basin ,Geology ,Nappe - Abstract
Great progress has been made in middle Permian exploration in Northwest Sichuan in recent years, but there are still many questions in understanding the hydrocarbon accumulation conditions. Due to the abundance of source rocks and the multi-term tectonic movements in this area, the hydrocarbon accumulation model is relatively complex, which has become the main problem to be solved urgently in oil and gas exploration. Based on the different tectonic backgrounds of the middle Permian in northwest Sichuan Basin, the thrust nappe belt, the hidden front belt, and the depression belt are taken as the research units to comb and compare the geologic conditions of the middle Permian reservoir. The evaluation of source rocks and the comparison of hydrocarbon sources suggest that the middle Permian hydrocarbon mainly comes from the bottom of the lower Cambrian and middle Permian, and the foreland orogeny promoted the thermal evolution of Paleozoic source rocks in northwest Sichuan to high maturity and over maturity stage. Based on a large number of reservoir physical properties data, the middle Permian reservoir has the characteristics of low porosity and low permeability, among which the thrust nappe belt and the hidden front belt have relatively high porosity and relatively developed fractures. The thick mudstone of Longtan formation constitutes the regional caprock in the study area and the preservation condition is good as a whole. However, the thrusting faults destroyed the sealing ability of the caprock in the nappe thrust belt. Typical reservoir profiles revealed that the trap types were different in the study area. The thrust fault traps are mainly developed in the thrust nappe belt, while the fault anticline traps are developed in the hidden front belt, and the structural lithological traps are developed in the depression belt. The different structural belts in northwest Sichuan have different oil and gas accumulation models, this paper built three hydrocarbon accumulation models by the analysis of reservoir formation conditions. The comprehensive analysis supposed the hidden front belt is close to the lower Cambrian source rock, and the reservoir heterogeneity is weak, faults connected source rock is developed, so it is a favorable oil and gas accumulation area in the middle Permian.
- Published
- 2021
154. Fatigue damage analysis of cement stabilized sub-base during construction period
- Author
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Qiqi Li, Naren Fang, Shiqing Hu, Lei Xin, and Xuancang Wang
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Cement ,Computer Science (miscellaneous) ,Period (geology) ,Environmental science ,Geotechnical engineering ,Fatigue damage ,Engineering (miscellaneous) - Published
- 2021
155. TGF-β1 controls porcine granulosa cell states: A miRNA-mRNA network view
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Kerong Shi, Xing Du, Qifa Li, Lingfang Wang, and Qiqi Li
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Ovarian Granulosa Cell ,Swine ,Granulosa cell ,Cell ,Biology ,Transforming Growth Factor beta1 ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Food Animals ,microRNA ,medicine ,Animals ,RNA, Messenger ,Small Animals ,Granulosa Cells ,030219 obstetrics & reproductive medicine ,Equine ,0402 animal and dairy science ,Wnt signaling pathway ,04 agricultural and veterinary sciences ,Cell cycle ,040201 dairy & animal science ,Cell biology ,MicroRNAs ,medicine.anatomical_structure ,Female ,Animal Science and Zoology ,Signal transduction ,Signal Transduction - Abstract
TGF-β1, an important multi-functional cytokine of the TGF-β signaling pathway, has been reported to be crucial for ovarian granulosa cell (GC) states and female fertility. However, the molecular mechanism underlying TGF-β1 regulation of GC states remains largely unknown. Here, we provide a comprehensive transcriptomic view on TGF-β1 regulation of cell states in porcine GCs. We first confirmed that TGF-β1 can control GC states (apoptosis and proliferation) in pig ovary. RNA-seq showed that 909 differentially expressed genes (DEGs), including 890 DEmRNAs and 19 DEmiRNAs, were identified in TGF-β1-treated porcine GCs. Functional annotation showed that these DEGs were mainly involved in regulating cell states. In addition, multiple hub genes were identified by constructing the protein-protein interaction network, DEmiRNA-DEmRNAs regulatory network, and gene-pathway-function co-expression networks, which were further found to be enriched in FoxO, TGF-β, Wnt, PIK3-Akt, p53 and Ras signaling pathways that play important roles in regulating cell states, cell cycle, proliferation, stress-responses and inflammation. The current research deeply reveals the effects of TGF-β1 on porcine GCs, and also identifies potential therapeutic RNA molecules for inhibiting and rescuing female infertility.
- Published
- 2021
156. Physics Identity of Chinese Students Before and After Gaokao: the Effect of High-Stake Testing
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Jianlan Wang, Ying Luo, and Qiqi Li
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Perception ,media_common.quotation_subject ,Affective learning ,Mathematics education ,Identity (social science) ,Context (language use) ,China ,Science education ,Education ,Test (assessment) ,media_common ,Educational systems - Abstract
High-stakes testing (HST) is critical in the educational system of East-Asian countries like China, and the ongoing standards-based reform in many Western countries like the USA. HST has been criticized for corrupting an educational system through means like disengaging students from authentic learning. It seems that students’ affective learning would be promoted if HST were abandoned. In this study, we used the mixed-method approach to investigate this hypothetical question by comparing the physics identity of high-school students in an HST-oriented educational context and college students who have transferred from an HST-oriented context to an almost HST-free one in China. We administered a physics identity survey with 108 students from two physics classes in a key high school and 233 undergraduate physics majors in a top-tier university who were separated by Gaokao as the most life-shaping test in China. We also interviewed sample participants about their physics learning. The data show the freshmen in college had a significantly higher average physics identity than the high-school students did. In the undergraduate physics program, the average physics identity had a deteriorating pattern from the freshmen, sophomores, to juniors. From high school to college, the students’ intention of pursuing physics as a major or career was similarly low. Differently, the students’ perception of lecturing changed, so did their self-discipline. Together, the findings suggested that simply discarding HST would not promote students’ affective learning. An alternative was needed to replace the extrinsic pressure from HST, which could be either students’ intrinsic engine of learning or another rewarding or coercive system.
- Published
- 2020
157. Pseudokineococcus galaxeicola sp. nov., isolated from mucus of a stony coral
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Anjie Guo, Yicong Zheng, Qiqi Li, Yu Chen, Si Zhang, and Jie Li
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Phosphatidylglycerol ,biology ,Strain (chemistry) ,Phylogenetic tree ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,Microbiology ,Mucus ,chemistry.chemical_compound ,chemistry ,Genus ,Galaxea ,Ecology, Evolution, Behavior and Systematics ,Bacteria - Abstract
A Gram-stain-positive, aerobic, motile, coccus-shaped bacterium, designated strain SCSIO 13315T, was isolated from mucus of the coral Galaxea sp. collected from Luhuitou fringing reef (Sanya, Hainan, PR China). Analysis of the 16S rRNA gene sequence showed that strain SCSIO 13315T exhibits 95.5 % 16S rRNA gene sequence similarity to Pseudokineococcus basanitobsidens SKC1-2T, 95.8 % to Pseudokineococcus lusitanus T2A-S27T and 96.4 % to Pseudokineococcus marinus KST3-3T. Results of phylogenetic analysis suggested that strain SCSIO 13315T was a member of the genus Pseudokineococcus . The DNA G+C content of strain SCSIO 13315T was 73.5 %. Chemotaxonomic assessment of strain SCSIO 13315T showed that the menaquinones were MK-8(H4) and MK-9(H2). The main cellular fatty acids were anteiso-C15 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c). The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids, four unidentified aminolipids and one unidentified lipid. Based on the phylogenetic and phenotypic analysis, it was evident that strain SCSIO 13315T represents a novel species of the genus Pseudokineococcus , for which the name Pseudokineococcus galaxeicola sp. nov. is proposed. The type strain is SCSIO 13315T (=NBRC 109944T=DSM 27812T).
- Published
- 2020
158. miR-130a/TGF-β1 axis is involved in sow fertility by controlling granulosa cell apoptosis
- Author
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Xing Du, Wangjun Wu, Peng Shang, Qiqi Li, Lingfang Wang, Qifa Li, Yangzom Chamba, and Zengxiang Pan
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Swine ,Population ,Apoptosis ,Ovary ,Biology ,medicine.disease_cause ,Transforming Growth Factor beta1 ,03 medical and health sciences ,0302 clinical medicine ,Food Animals ,microRNA ,Follicular phase ,medicine ,Animals ,Luciferase ,Small Animals ,education ,Gene ,Mutation ,education.field_of_study ,Granulosa Cells ,030219 obstetrics & reproductive medicine ,Equine ,0402 animal and dairy science ,Promoter ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,Cell biology ,MicroRNAs ,Fertility ,medicine.anatomical_structure ,Gene Expression Regulation ,Female ,Animal Science and Zoology ,Signal Transduction - Abstract
TGF-β1 is a ligand of the TGF-β superfamily and an important cytokine that regulates ovarian functions including follicular development, steroid production, ovulation, luteinization, and female fertility. However, little is known about the regulation of TGF-β1 expression in ovary. Here, we identified that TGF-β1 is a functional target of miR-130a in porcine ovarian granulosa cells (GCs). The 3′-UTR sequence of TGF-β1 gene (1137 bp in length) in Large White (LW) pig was isolated, and multiple RNA regulatory elements (RREs), including several binding motifs of different miRNAs, were identified in this region. Luciferase activity assay showed that miR-130a dramatically suppresses the 3′-UTR luciferase activity of TGF-β1 gene, and further inhibits the expression of TGF-β1 in porcine GCs. FACS revealed that miR-130a acts as a pro-apoptotic factor and promotes GC apoptosis by inhibiting TGF-β1. Two novel linked mutations (−573G > A and −540T > C) were identified in the promoter region of ssc-miR-130a, but their polymorphisms are not associated with sow reproductive traits. Importantly, combined genotype analysis with a known mutation (c.1583 A > G) in the 3′-UTR of porcine TGF-β1 gene showed a significant association with reproductive performance in LW sow population. Overall, our findings defined a novel regulatory axis, miR-130a/TGF-β1 axis, which is involved in regulating sow fertility.
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- 2020
159. Huber Loss Function Based on t-Distribution Yin-Yang-Pair Optimization Algorithm
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Qiqi Li, Fei'er Yang, Ruoxi Wei, Han Sun, Zan Yang, and Wei Nai
- Published
- 2022
160. Improving the surface area of metal organic framework-derived porous carbon through constructing inner support by compatible graphene quantum dots
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Tingting Tang, Renlu Yuan, Nannan Guo, Jiayao Zhu, Xuemeng Gan, Qiqi Li, Fuwei Qin, Wanxia Luo, Luxiang Wang, Su Zhang, Huaihe Song, and Dianzeng Jia
- Subjects
Biomaterials ,Colloid and Surface Chemistry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Abstract
Metal-organic frameworks (MOFs) have emerged as promising precursors to prepare porous carbons due to their unique coordination structure with abundant pores and various chemical compositions. However, the structural collapse and pore shrinkage during pyrolysis severely decrease the surface area of the prepared porous carbons. Herein, we propose an inner support strategy to prepare MOF-derived carbons with improved surface area using graphene quantum dots (GQDs) as the compatible frameworks. GQDs with abundant carboxyl groups (-COOH) and rigid structure can uniformly distribute in MOF-5 precursor by coordinating with [Zn
- Published
- 2022
161. Structural Equation Model for Evaluating Factors Affecting Cyber-Violence of College Students.
- Author
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Yongwei Yang and Qiqi Li
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STRUCTURAL equation modeling ,COLLEGE students ,FACTOR analysis ,BEHAVIORAL assessment ,PSYCHOLOGICAL factors ,HOMICIDE rates - Abstract
As the main entertainment place for human beings, the hidden problems of the Internet are becoming more and more obvious, of which cyber-violence is one of the most typical problems at present. When studying the factors that influence cyber violence, various technologies and methods are used to explain the relationships between specific variables. Unexpectedly, the structural equation model (SEM) has acquired very little concern in factors affecting cyber-violence incidents. In order to solve the limitations of this knowledge body, this study is to establish a model by using structural equation modeling to determine the key factors affecting college students' cyber-violence. In this paper, by designing a questionnaire to obtain relevant data, five potential influencing factors of cyber violence are quantified, including netizens' behavior factors, moral sentiment factors, netizens' psychological factors, social environment factors and behavior analysis factors. On the basis of excavating the multi-layer complex interactive relationship between the influencing factors of cyber violence, ten hypothetical relationships are established and compared by using a structural equation model. Through the analysis of the influencing factors of cyber-violence, it is recognized that reasonably guiding cyber groups is one of the important way to solve extreme mass incidents. [ABSTRACT FROM AUTHOR]
- Published
- 2023
162. Perceptions of biomass energy sustainability in policy scenarios of China
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Xiaoyu Wang, Qiqi Liu, Lu Yang, Qing Yang, Yujiao Li, Yu Yang, Chao Zhou, Xu Wang, Yang Wang, Guofu Gao, Wenxiang Liu, and Ping Cheng
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Bioenergy ,Biomass resource management ,Biomass energy products ,Industry standard ,Ecological energy agriculture demonstration zone ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
This study examines biomass energy policies in the EU, US, and Japan, noting high implementation rates in Poland (86.5 %) and Finland (90.6 %). Germany's biogas utilization is particularly noteworthy, accounting for 29.6 %. The paper summarizes China's national and provincial waste biomass management and energization policies, encompassing agriculture, biomass energy, and environmental governance aspects. Analyzing China's biomass energy industry reveals challenges requiring a comprehensive development plan based on waste biomass resources and environmental zoning. Proposed solutions include establishing ecological energy agriculture demonstration zones, optimizing policies for environmental benefits, encouraging technological innovation, establishing a trade association, improving standards, setting up a waste biomass fund, introducing green certificates and quotas, and integrating waste biomass into the national carbon trading system.
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- 2024
- Full Text
- View/download PDF
163. Higher adaptive tolerance with higher risk for sparrows living in airport environments
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Laikun Ma, Qiqi Liu, Jiaojiao Wang, and Jianhua Hou
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Flight initiation distance ,Airport ,Group size ,Passer montanus ,Bird strikes ,Ecology ,QH540-549.5 - Abstract
Adjustments in flight initiation distance (FID) indicate how birds adapt to external environmental changes, including semi-natural ecosystems such as airports with regular disturbances. This study focused on sparrows (Passer montanus) as the research subject, because it is a dominant species with a high risk of bird strike events at airports. We investigated the FID and its influencing factors both inside and outside an airport compound to determine sparrows' vigilance and their adaptive adjustments to the airport environment. The FID of sparrows showed no significant variation between inside and outside the airport compound. However, a significant reduction of FID was exhibited during the breeding season. Group size showed a significant positive impact on the FID, somewhat supporting the many-eyes hypothesis, in which larger groups with more individuals may detect predators earlier. Additionally, the stimulus type and location influenced the effect of group size on FID, with most birds showing greater tolerance to vehicles than to humans, and being slightly bolder inside the airport than outside it. Overall, sparrows showed a higher tolerance to disturbance inside the airport compound and less vigilance to vehicles than to humans. Nevertheless, these adaptations may inadvertently form an ecological trap for bird populations around airports, exposing them to the dual threats of higher levels of raptor predation and the hazards of bird strikes. Thus multifaceted bird-scaring strategies and measures should be formulated to ensure flight safety and enhance avian survival during airport management.
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- 2024
- Full Text
- View/download PDF
164. Novel eIF4E/eIF4G protein-protein interaction inhibitors DDH-1 exhibits anti-cancer activity in vivo and in vitro
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Hui Yang, Qiqi Li, Shuhong Zhang, Chuanlong Guo, Junting Fan, Jun Wang, and Lijun Wang
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Lung Neoplasms ,MAP Kinase Signaling System ,DNA damage ,Mice, Nude ,Antineoplastic Agents ,Apoptosis ,02 engineering and technology ,Biochemistry ,Flow cytometry ,Mice ,03 medical and health sciences ,Cyclin D1 ,Structural Biology ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Protein Interaction Maps ,Phosphorylation ,Molecular Biology ,Cell Proliferation ,030304 developmental biology ,Membrane Potential, Mitochondrial ,chemistry.chemical_classification ,A549 cell ,Mice, Inbred BALB C ,0303 health sciences ,Reactive oxygen species ,medicine.diagnostic_test ,General Medicine ,021001 nanoscience & nanotechnology ,In vitro ,Cell biology ,Thiazoles ,Eukaryotic Initiation Factor-4E ,chemistry ,A549 Cells ,Caspases ,Eukaryotic Initiation Factor-4G ,Reactive Oxygen Species ,0210 nano-technology ,DNA Damage ,Signal Transduction - Abstract
(E)-2-(2-(2,3-dibromo-4,5-dimethoxybenzylidene)hydrazinyl)-4-(3,4-difluorophenyl) thiazole (DDH-1) is novel small molecule compound synthesized in our previous work. This study found that DDH-1 could inhibit the proliferation of various human lung cancer cells. Particularly, the IC50 for A549 cells was 8.59 μM. Interestingly, we found that DDH-1 inhibits eIF4E/eIF4G interaction. The flow cytometry (FACS) results have indicated that DDH-1 may induce G0/G1 cycle arrest by inhibiting the expression of Cyclin D1 and CDK4. DDH-1 may also cause apoptosis through the caspase-dependent pathway. Study of these mechanisms has shown that DDH-1 may prompt reactive oxygen species (ROS) generation, decrease the mitochondrial membrane potential (MMP), and stimulate DNA double-strand breaks (DSBs) in A549 cells. Study of the signal pathway has indicated that DDH-1 could activate JNK phosphorylation and inhibit ERK phosphorylation. Interestingly, NAC, which scavenges ROS, reversed the MMP decline, DNA damage, JNK phosphorylation activation, and ERK phosphorylation inhibition caused by DDH-1. Overall, these findings provide evidence that the eIF4E/eIF4G interaction inhibitors DDH-1 induces DNA damage and apoptosis in human lung cancer A549 cells.
- Published
- 2020
165. Rebaudioside A/TPGS mixed nanomicelles as promising nanocarriers for nimodipine ocular delivery
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Jingwang Fang, Xianggen Wu, Hui Yang, Qiqi Li, Jun Wang, Xuefei Li, and Meng Xin
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Intraocular pressure ,genetic structures ,Membrane permeability ,Pharmaceutical Science ,Glaucoma ,02 engineering and technology ,Polyethylene glycol ,Pharmacology ,021001 nanoscience & nanotechnology ,medicine.disease ,030226 pharmacology & pharmacy ,Micelle ,eye diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,chemistry ,In vivo ,medicine ,sense organs ,Nanocarriers ,0210 nano-technology ,Nimodipine ,medicine.drug - Abstract
Nimodipine (NMD), a calcium channel blocker, has demonstrated benefits in treating glaucoma. However, its ocular therapeutic application remains limited due to its poor aqueous solubility, which restrains the development of an ophthalmic formulation. Thus, the present study aimed to formulate an NMD micelle ophthalmic solution to enhance the potential of NMD in an ocular topical formulation to treat glaucoma. The NMD micelle ophthalmic solution was formulated with nanocarriers composed of rebaudioside A and d-α-tocopheryl polyethylene glycol 1000 succinate. Spherical mixed micelles were optimized and obtained at a small micelle size 13.429 ± 0.181 nm with a narrow size distribution (polydispersity index 0.166 ± 0.023) and high encapsulation efficiency rate (99.59 ± 0.09%). Compared with free NMD, NMD in micelles had much greater in vitro membrane permeability and antioxidant activity. The NMD micelle ophthalmic solution was well tolerated in rabbit eyes. It profoundly improved the in vivo intraocular permeation of NMD, and in vivo intraocular pressure reduction and improved miosis were also observed. Accordingly, this NMD micelle ophthalmic solution might be a promising ocular formulation to treat glaucoma.
- Published
- 2020
166. Covid-19’s adverse effects on a stock market index
- Author
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Yun Liu, Chi Keung Woo, Qiqi Li, and Kang Hua Cao
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Economics and Econometrics ,2019-20 coronavirus outbreak ,050208 finance ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,0502 economics and business ,05 social sciences ,Econometrics ,Business ,050207 economics ,Adverse effect ,Stock market index ,Panel data - Abstract
We perform a panel data analysis of 14 daily stock market indices during 01/21/2020 – 06/30/2020 to document a stock market index’s negative responsiveness to Covid-19’s spread variations. We find ...
- Published
- 2020
167. The Effect of p53-R249S on the Suppression of Hepatocellular Carcinoma Cells Survival Induced by Podophyllum Derivatives
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Huan Chen, Mingyang Zhang, Lingqi Li, Huai Wang, Ziping Wang, and Qiqi Li
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Cancer Research ,Carcinoma, Hepatocellular ,Cell Line ,Structure-Activity Relationship ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,medicine ,Humans ,MTT assay ,Cell Proliferation ,030304 developmental biology ,Pharmacology ,Biological Products ,0303 health sciences ,Dose-Response Relationship, Drug ,Molecular Structure ,medicine.diagnostic_test ,biology ,Chemistry ,Cell growth ,Liver cell ,Liver Neoplasms ,Podophyllum ,Cancer ,medicine.disease ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,Molecular Docking Simulation ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Cancer research ,Molecular Medicine ,Drug Screening Assays, Antitumor ,Tumor Suppressor Protein p53 ,Podophyllum peltatum - Abstract
Background: Hepatocellular Carcinoma (HCC), the second leading cause of cancer-related mortality with over half a million new cases diagnosed annually in the world, accounts for nearly 70% of cancer deaths in parts of Asia and Africa. Podophyllum, one of the important members of the lignane class of natural products derived from plants in Podophyllum peltatum L., has been shown to suppress tumor growth in various cancers. However, the effects of Podophyllum compounds on HCC and the mechanisms for its tumor-suppressive function remain unknown. Methods: A molecular docking study was employed to the analysis of the interaction between compounds and their targeted proteins. Cell proliferation was measured by MTT assay. Western blot analysis was used to evaluate protein expression. qRT-PCR was performed to assess RNA expression. Results: Molecular docking analysis was consistent with the beneficial effect of fluorine atom substituent in the 3-position of 2-aminopyridine in our previous study. Also, P-3F and D-3F displayed the most potent cytotoxicities against PLC/PRF/5 with p53-R249S and weakest inhibition of L02 (normal liver cell) growth. However, these derivatives had no effect on the suppression of HepG2 (wild-type p53) and Hep3B (p53-null) proliferation significantly. Further study showed that both compounds increase γ-H2AX expression in PLC/PRF/5 cell, along with repression of the c-Myc activation, purportedly by induction of p53 level and transcriptional activation. Conclusion: The results suggested that podophyllum derivatives containing fluorine atom in the 3-position of 2- aminopyridine could inhibit the growth of HCC harboring p53-R249S by restoring the activity of p53 with decreasing the level of c-Myc.
- Published
- 2020
168. Adsorption/Reduction Behaviors of Modified Cellulose Aerogels for the Removal of Low Content of Cr(VI)
- Author
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Minghua Liu, Jianting Liu, Yifan Liu, Yuancai Lv, Xiaoxia Ye, Ruihan Pei, Guifang Yang, Minya Wu, Tong Shengxuan, Qiong Wu, and Qiqi Li
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Environmental Engineering ,Materials science ,Polymers and Plastics ,Langmuir adsorption model ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Grafting ,chemistry.chemical_compound ,symbols.namesake ,Adsorption ,020401 chemical engineering ,chemistry ,Monolayer ,Materials Chemistry ,Water environment ,symbols ,Chelation ,0204 chemical engineering ,Cellulose ,0210 nano-technology ,Dissolution ,Nuclear chemistry - Abstract
Removal of low content of Cr(VI) from water is a critical challenge for water environment protection. Herein, an acrylamide-thiosemicarbazide cellulose aerogels (ATC) was designed by dissolving, grafting and freeze-drying to introduce thiosemicarbazide onto acrylamide-cellulose aerogels (AMC) via Schiff based reaction. The resultant ATC was further employed as an efficient adsorbent to remove low content of Cr(VI) from wastewater by adsorption and reduction. The SEM, FT-IR, and BET results showed that the ATC was composed of well-developed pore structure and owned functional groups such as –NH2, C–S and C=S groups. Experimental results indicated that ATC exhibited excellent removal efficiency for 99% under low initial concentration (0.1–10 mg/L). The adsorption isotherms demonstrated ATC adsorbent according to a monolayer adsorption behavior following the Langmuir model with a calculated maximum adsorption capacity of 83.40 mg/g at pH 3, and the adsorption kinetics well obeyed the pseudo-first-order kinetic model. Importantly, the maximum effluent concentration of Cr(VI) was lower than 0.045 mg/L, meeting the standard of WHO (50 μg/L). Furthermore, the main removal mechanisms involved the synergistic effect of electrostatic interactions, reduction and chelation between the positively charged functional groups of adsorbent and the anionic Cr(VI) ions.
- Published
- 2020
169. SMAD4 activates Wnt signaling pathway to inhibit granulosa cell apoptosis
- Author
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Qiqi Li, Xing Du, Lu Liu, Qifa Li, Liu Yang, and Qiuyu Cao
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Cancer Research ,Cell type ,Reproductive disorders ,Follicular Atresia ,Immunology ,Apoptosis ,Biology ,Article ,Cellular and Molecular Neuroscience ,Transcription (biology) ,Humans ,lcsh:QH573-671 ,Promoter Regions, Genetic ,Wnt Signaling Pathway ,Transcription factor ,Smad4 Protein ,Granulosa Cells ,Effector ,lcsh:Cytology ,Follicular atresia ,Wnt signaling pathway ,Cell Biology ,Cell biology ,MicroRNAs ,Crosstalk (biology) ,Gene Expression Regulation ,Female ,Signal transduction - Abstract
The TGF-β and Wnt signaling pathways are interrelated in many cell types and tissues, and control cell functions in coordination. Here, we report that SMAD4, a downstream effector of the TGF-β signaling pathway, induces FZD4, a receptor of the Wnt signaling pathway, establishing a novel route of communication between these two pathways in granulosa cells (GCs). We found that SMAD4 is a strong inducer of FZD4, not only initiating FZD4 transcription but also activating FZD4-dependent Wnt signaling and GC apoptosis. Furthermore, we identified the direct and indirect mechanisms by which SMAD4 promotes expression of FZD4 in GCs. First, SMAD4 functions as a transcription factor to directly bind to the FZD4 promoter region to increase its transcriptional activity. Second, SMAD4 promotes production of SDNOR, a novel lncRNA that acts as a sponge for miR-29c, providing another mean to block miR-29c from degenerating FZD4 mRNA. Overall, our findings not only reveal a new channel of crosstalk between the TGF-β and Wnt signaling pathways, SMAD4–FZD4 axis, but also provide new insights into the regulatory network of GC apoptosis and follicular atresia. These RNA molecules, such as miR-29c and lnc-SDNOR, represent potential targets for treatment of reproductive diseases and improvement of female fertility.
- Published
- 2020
170. NORFA, long intergenic noncoding RNA, maintains sow fertility by inhibiting granulosa cell death
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Qiqi Li, Lifan Zhang, Xing Du, Zengxiang Pan, Qifa Li, and Lu Liu
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endocrine system ,Cell biology ,Granulosa cell ,Follicular Atresia ,Sus scrofa ,Medicine (miscellaneous) ,Apoptosis ,General Biochemistry, Genetics and Molecular Biology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Transcription (biology) ,Animals ,Epigenetics ,Promoter Regions, Genetic ,Transcription factor ,3' Untranslated Regions ,lcsh:QH301-705.5 ,Cells, Cultured ,030304 developmental biology ,Regulation of gene expression ,0303 health sciences ,Binding Sites ,Granulosa Cells ,biology ,Follicular atresia ,Receptor, Transforming Growth Factor-beta Type II ,Non-coding RNA ,NFIX ,Gene regulation ,MicroRNAs ,NFI Transcription Factors ,Fertility ,Gene Expression Regulation ,lcsh:Biology (General) ,030220 oncology & carcinogenesis ,biology.protein ,Female ,RNA, Long Noncoding ,General Agricultural and Biological Sciences ,Signal Transduction - Abstract
Long intergenic non-coding RNAs (lincRNAs) have been proved to be involved in regulating female reproduction. However, to what extent lincRNAs are involved in ovarian functions and fertility is incompletely understood. Here we show that a lincRNA, NORFA is involved in granulosa cell apoptosis, follicular atresia and sow fertility. We found that NORFA was down-regulated during follicular atresia, and inhibited granulosa cell apoptosis. NORFA directly interacted with miR-126 and thereby preventing it from binding to TGFBR2 3′-UTR. miR-126 enhanced granulosa cell apoptosis by attenuating NORFA-induced TGF-β signaling pathway. Importantly, a breed-specific 19-bp duplication was detected in NORFA promoter, which proved association with sow fertility through enhancing transcription activity of NORFA by recruiting transcription factor NFIX. In summary, our findings identified a candidate lincRNA for sow prolificacy, and provided insights into the mechanism of follicular atresia and female fertility., Xing Du et al. show that long intergenic non-coding RNA (lincRNA) NORFA maintains sow fertility by inhibiting apoptotic death of granulosa cells through the TGF-β signaling pathway. A breed-specific 19-bp duplication suggests that transcription factor NFIX may enhance the transcription of NORFA, providing insights into the role of lincRNAs for female fertility.
- Published
- 2020
171. Carbon materials for high mass-loading supercapacitors: filling the gap between new materials and practical applications
- Author
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Huaihe Song, Yue Dong, Jiayao Zhu, Su Zhang, Qiqi Li, and Dianzeng Jia
- Subjects
Supercapacitor ,Materials science ,Renewable Energy, Sustainability and the Environment ,chemistry.chemical_element ,New materials ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Engineering physics ,Capacitance ,0104 chemical sciences ,chemistry ,Electrode ,High mass ,General Materials Science ,0210 nano-technology ,Carbon ,Electrical conductor - Abstract
The ever-increasing demands for low-cost, miniaturized, light-weight supercapacitors significantly enhance the standards of electrode materials. High capacitance and good rate performance at high mass loadings (>10 mg cm−2) are crucial for increasing the energy density of supercapacitors under the premise of high-power characteristic. However, most electrode materials show good performance only at a rather low mass loading. Increasing the electrode mass loading or thickness causes severely blocked electron and ion transport channels, decreased accessible active sites, as well as rapid fading of the capacitance and rate performance. The gap between the materials' theoretical and practical electrochemical performances drastically hinders their application. In this review, we summarize the design and preparation of high mass-loading carbon electrodes from the aspects of the entire conductive network and ion transport channels, dense structural design, and freestanding electrode construction. The basic electrochemical processes and kinetics, structure–property relationships, and new energy storage sites are discussed towards filling the gap between the new materials and practical applications. Finally, the future prospects, challenges, and promising strategies to solve the current problems are concluded to shed light on the development of high-performance practical carbon electrode materials.
- Published
- 2020
172. Estimated Benefits of Incremental Suppression of Covid-19 Spread
- Author
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Qiqi Li, Kang Hua Cao, Yun Lyndon Liu, and Chi Keung Woo
- Subjects
Coronavirus disease 2019 (COVID-19) ,Cost–benefit analysis ,Statistics ,Panel data ,Mathematics - Abstract
Estimated benefits are necessary for a cost benefit analysis of Covid-19 suppression. We propose a stock-market-based approach to estimate the benefits of incremental suppression of Covid-19’s spread that will last till no new cases are recorded for 14 days, the projected incubation period of Covid-19. This approach’s empirical implementation uses a) total capitalization of 14 market indices for large cap stocks; and b) an index’s estimated elasticity of cumulative confirmed cases (CCC) obtained from a panel data analysis of 727 daily observations in the period of 01/21/2020-04/03/2020. Our estimated benefits of a hypothetical 10% reduction in CCC due to incremental suppression are statistically significant (p-value
- Published
- 2020
173. Temperature-Dependent Terahertz Spectra of Isonicotinamide in the Form I Studied Using the Quasi-Harmonic Approximation
- Author
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Yin Li, Yiwen Sun, Qiqi Li, Jiangtao Lei, Bo Yang, Yun Shen, Yingxiang Cai, and Xiaohua Deng
- Subjects
Niacinamide ,Terahertz Spectroscopy ,Molecular Conformation ,Temperature ,Physical and Theoretical Chemistry ,Vibration ,Atomic and Molecular Physics, and Optics - Abstract
Anharmonicity of molecular vibrational motions is closely associated with the thermal property of crystals. However, the origin of anharmonicity is still not fully understood. Low-frequency vibrations, which are usually defined in the terahertz (THz) range, show excellent sensitivity to anharmonicity. In this work, anharmonicity of isonicotinamide in the form I was investigated by using temperature-dependent terahertz time-domain spectroscopy and the quasi-harmonic approximation (QHA) approach at PBE-D3 and PBE-MBD levels. Both DFT calculations suggest the variation of π-π stacking conformation dominates in the thermal expansion of the unit cell. Frequency shifts of the modes in THz range obtained by QHA approach are found to be qualitatively consistent with experimental observations, demonstrating QHA approach is a useful tool for the interpretation of frequency shifts of modes induced by temperature.
- Published
- 2022
174. Visual P300 as a neurophysiological correlate of symptomatic improvement by a virtual reality-based computer AT system in patients with auditory verbal hallucinations: A Pilot study
- Author
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Nana Liang, Xinrong Li, Xianju Guo, Sha Liu, Yao Liu, Wentao Zhao, Yujiao Wen, Yue Li, Jing Li, Fangya Li, Haiyan Wu, Jianhong Li, Qiqi Li, Jianxia Guo, and Yong Xu
- Subjects
Psychiatry and Mental health ,Hallucinations ,Computers ,Quality of Life ,Virtual Reality ,Humans ,Pilot Projects ,Biological Psychiatry - Abstract
Previous comparative trials showed that virtual reality (VR) therapies achieved larger effects than gold-standard cognitive-behavioral therapy (CBT) on overall auditory verbal hallucinations (AVHs). However, no trial has examined the corresponding underlying electrophysiological mechanisms. We performed a pilot randomized comparative trial evaluating the efficacy of a virtual reality-based computer AT system (CATS) over CBT for schizophrenia (SCZ) patients with treatment-resistant AVHs and explored these potential electrophysiological changes via the visual P300 component. Patients (CATS, n = 32; CBT, n = 33) completed the clinical assessments pre- and post-interventions and at 12-week follow-up. The visual P300 were measured before and after both therapies. The analysis of changes in psychiatric symptoms used linear mixed-effects models, and the P300 response in temporal and time-frequency domains was analyzed with repeated-measures analysis of variance. There was no interaction effect between change in clinical symptoms and treatment group. However, several statistically significant within-group improvements were found for CATS and CBT over time. AVH improved significantly after both treatments, as measured with the Psychotic Symptom Rating Scales-Auditory Hallucinations (PSYRATS-AH) sub-scores. Especially for the CATS group, omnipotence beliefs, anxiety symptoms, self-esteem, and quality of life also remained improved at the 12-week follow-up. Moreover, P300 amplitude had a significant interaction effect and correlation with AVH response. Overall, our analysis did not demonstrate general clinical superiority of CATS over CBT, but CATS improved refractory AVH in SCZ patients, likely by increasing P300 amplitude. These findings support the continued development of CATS for persistent AVH and suggest further trials to clarify the neurological effects of CATS.
- Published
- 2022
175. How to Motivate Consumers’ Intention to Use Shared Electric Vehicles? From the Perspective of Institutional Theory
- Author
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Manzhi Liu, Xiangxue Meng, Chunxiu Zhang, Runrun Xiao, Qiqi Li, Heng Shi, and Jiaxin Zhang
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
176. Copolymer Micelle-administered Melatonin Ameliorates Hyperosmolarity-induced Ocular Surface Damage through Regulating PINK1-mediated Mitophagy
- Author
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Jing Xu, Peng Chen, Guangfen Zhao, Susu Wei, Qiqi Li, Chuanlong Guo, Qilong Cao, Xianggen Wu, and Guohu Di
- Subjects
Mice ,Cellular and Molecular Neuroscience ,Ophthalmology ,Polymers ,hemic and lymphatic diseases ,Mitophagy ,Animals ,Reactive Oxygen Species ,Protein Kinases ,Micelles ,Sensory Systems ,Melatonin - Abstract
To investigate the role and mechanism of melatonin-loaded polymer polyvinyl caprolactam���polyvinyl acetate���polyethyleneglycol graft copolymer micelles (Mel-Mic) in dry eye disease (DED). In vitro, the apoptosis and reactive oxygen species (ROS) generation in human corneal epithelial cells (HCECs) were analyzed by immunostaining and flow cytometry. The effect of Mel-Mic on autophagy and mitophagy was evaluated by immunostaining and western blots. PINK1 knockdown was analyzed by small interfering RNA. In vivo, sodium fluorescein staining, tear secretion test, and periodic acid-Schiff staining were used to determine whether Mel-Mic can alleviate the severity of DED. Small molecule antagonists were pretreated to investigate whether melatonin type 1 and/or 2 receptors (MT1/MT2) mediate the effects of Mel-Mic. Mel-Mic improved the solubility and biological activities of Mel in aqueous solutions. Treatment with Mel-Mic decreased the apoptosis of HCECs exposed to hyperosmotic medium, accompanied by downregulation of cleaved Caspase-3 and upregulation of Bcl-2. In addition, Mel-Mic application suppressed ROS overproduction, rescued mitochondrial function, and decreased the level of oxidative stress associated biomarkers (COX-2 and 4-HNE) in HCECs. Interestingly, HCECs treated with Mel-Mic exhibited increased levels of mitophagy markers (PINK1, PARKIN, Beclin 1, and LC3B) and restored impaired mitophagic flux under hyperosmolarity. While PINK1 knockdown largely abolished its protective effects. In vivo, compared to vehicle group, topical Mel-Mic-solution-treated mice showed significantly improved clinical parameters, increased tear production, and decreased goblet cells loss in a dose-dependent manner. Also, transmission electron microscopic assay revealed increased autophagosome number in the corneal epithelium of Mel-Mic group. Moreover, luzindole, a nonselective MT1/MT2 antagonist, but not 4-P-PDOT, a selective MT2 antagonist, blocked the protective effect of Mel-Mic. Our findings demonstrated that Mel-Mic ameliorates hyperosmolarity-induced ocular surface damage via PINK1-mediated mitophagy and may represent an effective treatment for DED possibly through acting MT1 receptor.
- Published
- 2022
- Full Text
- View/download PDF
177. Sowing Single Atom Seeds: A Versatile Strategy for Hyper‐Low Noble Metal Loading to Boost Hydrogen Evolution Reaction
- Author
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Qiqi Li, Qin Zhang, Wenli Xu, Rong Zhao, Min Jiang, Yinhong Gao, Wenda Zhong, Kai Chen, Yongting Chen, Xuanke Li, and Nianjun Yang
- Subjects
Renewable Energy, Sustainability and the Environment ,General Materials Science - Published
- 2023
178. A Deep Sensing System for Monitoring the Health Status of Transmission Line Equipment Based on Self-Powered Sensors
- Author
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Beibei Guo, Qiqi Li, Weihua Jiang, Kai Lu, and Haoming Cheng
- Subjects
Electrical and Electronic Engineering ,Instrumentation - Published
- 2023
179. Parametric analysis on axial compression performance of bio-inspired porous lattice structures
- Author
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Qiqi Li, Lijia Wu, Lin Hu, Eric Li, Tiefang Zou, and Xin Liu
- Subjects
Mechanical Engineering ,Building and Construction ,Civil and Structural Engineering - Published
- 2023
180. Federated Multi-Agent Actor-Critic Learning Task Offloading in Intelligent Logistics
- Author
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Qiqi Li, Yaping Cui, Tao Song, and Linjiang Zheng
- Subjects
Computer Networks and Communications ,Hardware and Architecture ,Signal Processing ,Computer Science Applications ,Information Systems - Published
- 2023
181. Immobilization of Pb2+ and Cr3+ using bentonite-sulfoaluminate cement composites
- Author
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Zhanping Song, Guoxin Li, Qiqi Li, Yan Wang, Li Cao, Lulu Han, and Mengdie Niu
- Subjects
Materials science ,0211 other engineering and technologies ,Foundation (engineering) ,020101 civil engineering ,02 engineering and technology ,Building and Construction ,Cement composites ,0201 civil engineering ,Compressive strength ,021105 building & construction ,Cushion ,Bentonite ,Setting time ,General Materials Science ,Composite material ,Civil and Structural Engineering - Abstract
The contamination of Pb and Cr to the environment cannot be ignored. It is an urgent task to explore an efficient and cheap technique to treat Pb and Cr. Bentonite-sulfoaluminate cement composites (Bent-CSA) was prepared to solidify Pb2+ and Cr3+, and their leachability, setting time, and compressive strength were researched in this study. Then the hydration products were analyzed via XRD and SEM. The results show that Bent-CSA immobilized Pb2+ and Cr3+ efficiently, the compressive strength was slightly affected by Pb2+ and Cr3+, but that gradually decreased as bentonite content increased. Bent-CSA could be reused as foundation cushion and roadbed materials after solidifying Pb2+ and Cr3+.
- Published
- 2019
182. The inhibition of protein translation promotes tumor angiogenic switch
- Author
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Hui Luo, Yuge Shen, Weiting Liao, Qiqi Li, Ni Wu, Jian Zhong, Chaoxin Xiao, Jia Gan, Yun Yang, E. Dong, Guimin Zhang, Binrui Liu, Xiaozhu Yue, Lin Xu, Yan Liu, Chengjian Zhao, Qian Zhong, and Hanshuo Yang
- Subjects
Pulmonary and Respiratory Medicine ,Pediatrics, Perinatology and Child Health - Abstract
The ‘angiogenic switch’ is critical for tumor progression. However, the pathological details and molecular mechanisms remain incompletely characterized. In this study, we established mammal xenografts in zebrafish to visually investigate the first vessel growth (angiogenic switch) in real-time, by inoculating tumor cells into the perivitelline space of live optically transparent Transgenic (flk1:EGFP) zebrafish larvae. Using this model, we found that hypoxia and hypoxia-inducible factor (HIF) signaling were unnecessary for the angiogenic switch, whereas vascular endothelial growth factor A gene (Vegfa) played a crucial role. Mechanistically, transcriptome analysis showed that the angiogenic switch was characterized by inhibition of translation, but not hypoxia. Phosphorylation of eukaryotic translation initiation factor 2 alpha (Eif2α) and the expression of Vegfa were increased in the angiogenic switch microtumors, and 3D tumor spheroids, and puromycin-treated tumor cells. Vegfa overexpression promoted early onset of the angiogenic switch, whereas Vegfa knockout prevented the first tumor vessel from sprouting. Pretreatment of tumor cells with puromycin promoted the angiogenic switch in vivo similarly to Vegfa overexpression, whereas Vegfa knockdown suppressed the increase. This study provides direc and dynamic in vivo evidences that inhibition of translation, but not hypoxia or HIF signaling promotes the angiogenic switch in tumor by increasing Vegfa transcription.
- Published
- 2021
183. A nano-phytochemical ophthalmic solution for marked improvement of corneal wound healing in healthy or diabetic mice
- Author
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Qiqi Li, Meng Xin, Xianggen Wu, and Bo Lei
- Subjects
Cornea ,Mice ,Wound Healing ,Biomedical Engineering ,Epithelium, Corneal ,Medicine (miscellaneous) ,Animals ,General Materials Science ,Bioengineering ,Development ,Ophthalmic Solutions ,humanities ,Diabetes Mellitus, Experimental - Abstract
Aim: To formulate a novel nano-phytochemical ophthalmic solution to promote corneal wound healing. Methods: Dipotassium glycyrrhizinate (DG) and palmatine (PAL) were used to formulate this formulation marked as DG–PAL, and its efficacy and mechanisms for promoting corneal wound healing were evaluated in mice. Results: DG–PAL was easily fabricated with excellent physical profiles. In in vivo efficiency evaluations, DG–PAL demonstrated an excellent promoting effect on corneal epithelial/nerve wound healing in both healthy and diabetic mice. These effects were involved in the DG–PAL-induced decreased expression levels of HMGB1 and its signaling-related factors in the corneas and trigeminal neurons of the healthy or diabetic mice. Conclusion: DG–PAL possibly represents a promising ophthalmic solution for promoting corneal wound healing.
- Published
- 2021
184. A Study of Identifier Resolution Security Strategy Based on Security Domains
- Author
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Qiqi Li, Peng Wu, Ling Han, Danyang Bi, and Zheng Zeng
- Published
- 2021
185. A Study of Integration Path and Application of Active Identification Carriers
- Author
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Yin Sun, Qiqi Li, and Wanwan Zheng
- Published
- 2021
186. Improved effect of ultrasound-assisted enzymolysis on egg yolk powder: Structural properties, hydration properties and stability characteristics
- Author
-
Qiqi Li, Xinyue Zhang, Shitao Tang, Sijie Mi, Lizhi Lu, Qi Zeng, Minquan Xia, and Zhaoxia Cai
- Subjects
Solubility ,Eggs ,Egg Proteins ,General Medicine ,Powders ,Egg Yolk ,Food Science ,Analytical Chemistry - Abstract
Preparation of egg yolk powder (EYP) with excellent hydration properties and dissolution stability is important for the efficient utilization of its functional properties and nutritional properties in food. In this work, a new method utilizing ultrasound-assisted enzymolysis (UP-EM) was investigated to obtain EYP. Compared to enzymolysis-alone treated (EM), ultrasonic pre-treatment (UP) significantly increased the enzymatic hydrolysis rate by 106.28%. In particular, the UP 60 W 20 min-EM group obtained the desired solubility and coefficient of stability. The observed microstructure of EYP showed that the egg yolk particles obtained by UP-EM were more uniformly distributed and smaller in particle size. The protein structure was confirmed by Fourier transform infrared spectrum, which showed that UP enhanced the hydrogen bonding force between egg yolk proteins. Therefore, it can be believed that UP-EM can significantly improve the hydration properties and dispersion stability of EYP, laying the foundation for its wide applications in food industry.
- Published
- 2021
187. Axial compression performance of a bamboo-inspired porous lattice structure
- Author
-
Qiqi Li, Lijia Wu, Lin Hu, Tao Chen, Tiefang Zou, and Eric Li
- Subjects
Mechanical Engineering ,Building and Construction ,Civil and Structural Engineering - Published
- 2022
188. Novel Intelligent Navigation-aided Light Simulated Control System Based on Internet of Things
- Author
-
Deyin Zhang, Wenxuan Hong, Li Wang, and Qiqi Li
- Published
- 2021
189. Novel Transformer Harmonic Model and Its Application in Airport Power Supply System
- Author
-
Qiqi Li, Deyin Zhang, Tingting Liu, and Xi Zhang
- Published
- 2021
190. Novel Wing Icing Area Recognition Based on Morphological Processing Enhanced U-Net Network
- Author
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Deyin Zhang, Penghui Wei, Qiqi Li, and Mingwei Tan
- Published
- 2021
191. Low-frequency vibrational spectroscopy: a new tool for revealing crystalline magnetic structures in iron phosphate crystals
- Author
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Anish Ochani, Suling Shen, Yiwen Sun, Qiqi Li, Zihui Song, Michael T. Ruggiero, and Xudong Liu
- Subjects
Diffraction ,Materials science ,Terahertz radiation ,General Physics and Astronomy ,Infrared spectroscopy ,chemistry.chemical_element ,Ion ,chemistry ,Chemical physics ,Lithium ,Iron phosphate ,Physical and Theoretical Chemistry ,Spin (physics) ,Spectroscopy - Abstract
In this report, the strong-dependence of low-frequency (terahertz) vibrational dynamics on weak and long-range forces in crystals is leveraged to determine the bulk magnetic configuration of iron phosphate – a promising material for cathodes in lithium ion batteries. We demonstrate that terahertz time-domain spectroscopy – coupled with quantum mechanical simulations – can discern between various spin configurations in FePO4. Furthermore, the results of this work unambiguously show that the well-accepted space group symmetry for FePO4 is incorrect, and the low-frequency spectroscopic measurements provide a clearer picture of the correct structure over the gold-standard of X-ray diffraction. This work opens the door for characterizing, predicting, and interpreting crystalline magnetic ordering using low-frequency vibrational spectroscopy.
- Published
- 2021
192. A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments
- Author
-
Yang Wang, Wenjie Yuan, Siqi Guo, Qiqi Li, Xiaomei Chen, Cheng Li, Qianying Liu, Lei Sun, Zhenguo Chen, Zhenghong Yuan, Cheng Luo, Shijie Chen, Shuping Tong, Michael Nassal, Yu-Mei Wen, and Yong-Xiang Wang
- Subjects
Multidisciplinary ,Solubility ,Escherichia coli ,General Physics and Astronomy ,General Chemistry ,General Biochemistry, Genetics and Molecular Biology ,Molecular Chaperones ,Single-Chain Antibodies - Abstract
Single-chain variable fragments (scFvs), composed of variable domains of heavy and light chains of an antibody joined by a linker, share antigen binding capacity with their parental antibody. Due to intrinsically low solubility and stability, only two Escherichia coli-produced scFvs have been approved for therapy. Here we report that a 33-residue peptide, termed P17 tag, increases the solubility of multiple scFvs produced in Escherichia coli SHuffle strain by up to 11.6 fold. Hydrophilic sequence, especially charged residues, but not the predicted α-helical secondary structure of P17 tag, contribute to the solubility enhancement. Notably, the P17 tag elevates the thermostability of scFv as efficiently as intra-domain disulfide bonds. Moreover, a P17-tagged scFv targeting hepatitis B virus surface proteins shows over two-fold higher antigen-binding affinity and virus-neutralizing activity than the untagged version. These data strongly suggest a type I intramolecular chaperone-like activity of the P17 tag. Hence, the P17 tag could benefit the research, production, and application of scFv.
- Published
- 2021
193. Temperature dependent terahertz spectrum analysis and theoretical calculation of the Dapsone drugs
- Author
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Xudong Liu, Yiwen Sun, and Qiqi Li
- Subjects
Materials science ,Absorption spectroscopy ,Terahertz radiation ,CASTEP ,medicine ,Analytical chemistry ,Leprosy ,Dapsone ,Spectrum analysis ,medicine.disease ,medicine.drug - Abstract
Dapsone is a sulfonamides drug used to treat various types of leprosy and skin diseases. The temperature dependent absorption spectra of dapsone in the range of 0.3 to2.1THz were obtained. The Gaussian and CASTEP software was used respectively for molecular and crystal calculation of dapsone. The experimental results were compared with the calculated results, and the differences were analyzed in this study.
- Published
- 2021
194. An Inverse Node Graph-Based Method for the Urban Scene Segmentation of 3D Point Clouds
- Author
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Lujie Zou, Xianghong Hua, Weixing Xue, Cheng Li, Kegen Yu, Xiaoxing He, Hanwen Qi, Qiqi Li, and Bufan Zhao
- Subjects
point cloud semantic segmentation ,010504 meteorology & atmospheric sciences ,Computer science ,Science ,0211 other engineering and technologies ,Point cloud ,construction of graph ,02 engineering and technology ,01 natural sciences ,Cut ,Point (geometry) ,Segmentation ,021101 geological & geomatics engineering ,0105 earth and related environmental sciences ,graph cut ,business.industry ,Node (networking) ,Cognitive neuroscience of visual object recognition ,Pattern recognition ,Random forest ,General Earth and Planetary Sciences ,Graph (abstract data type) ,higher-order CRF optimization ,Artificial intelligence ,business - Abstract
Urban object segmentation and classification tasks are critical data processing steps in scene understanding, intelligent vehicles and 3D high-precision maps. Semantic segmentation of 3D point clouds is the foundational step in object recognition. To identify the intersecting objects and improve the accuracy of classification, this paper proposes a segment-based classification method for 3D point clouds. This method firstly divides points into multi-scale supervoxels and groups them by proposed inverse node graph (IN-Graph) construction, which does not need to define prior information about the node, it divides supervoxels by judging the connection state of edges between them. This method reaches minimum global energy by graph cutting, obtains the structural segments as completely as possible, and retains boundaries at the same time. Then, the random forest classifier is utilized for supervised classification. To deal with the mislabeling of scattered fragments, higher-order CRF with small-label cluster optimization is proposed to refine the classification results. Experiments were carried out on mobile laser scan (MLS) point dataset and terrestrial laser scan (TLS) points dataset, and the results show that overall accuracies of 97.57% and 96.39% were obtained in the two datasets. The boundaries of objects were retained well, and the method achieved a good result in the classification of cars and motorcycles. More experimental analyses have verified the advantages of the proposed method and proved the practicability and versatility of the method.
- Published
- 2021
195. IFN-γ surmounts PD-L1/PD1 inhibition to CAR-T cell therapy by upregulating ICAM-1 on tumor cells
- Author
-
Lin Shui, Hui Luo, Hanshuo Yang, Yun Yang, Qiqi Li, Xiao-zhu Yue, Wei Wang, Bin-rui Liu, and E. Dong
- Subjects
Cancer Research ,Letter ,Programmed Cell Death 1 Receptor ,Adaptive immunity ,lcsh:Medicine ,Tumor cells ,Immunotherapy, Adoptive ,B7-H1 Antigen ,Interferon-gamma ,Text mining ,Cell Line, Tumor ,Neoplasms ,PD-L1 ,Genetics ,Humans ,lcsh:QH301-705.5 ,ICAM-1 ,Receptors, Chimeric Antigen ,biology ,business.industry ,Chemistry ,lcsh:R ,Intercellular Adhesion Molecule-1 ,Neoplasm Proteins ,lcsh:Biology (General) ,Cancer research ,biology.protein ,CAR T-cell therapy ,Immunotherapy ,business - Published
- 2021
196. Secure Federated Evolutionary Optimization—A Survey
- Author
-
Qiqi Liu, Yuping Yan, Yaochu Jin, Xilu Wang, Peter Ligeti, Guo Yu, and Xueming Yan
- Subjects
Federated learning ,Privacy-preservation ,Security ,Evolutionary optimization ,Data-driven optimization ,Bayesian optimization ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
With the development of edge devices and cloud computing, the question of how to accomplish machine learning and optimization tasks in a privacy-preserving and secure way has attracted increased attention over the past decade. As a privacy-preserving distributed machine learning method, federated learning (FL) has become popular in the last few years. However, the data privacy issue also occurs when solving optimization problems, which has received little attention so far. This survey paper is concerned with privacy-preserving optimization, with a focus on privacy-preserving data-driven evolutionary optimization. It aims to provide a roadmap from secure privacy-preserving learning to secure privacy-preserving optimization by summarizing security mechanisms and privacy-preserving approaches that can be employed in machine learning and optimization. We provide a formal definition of security and privacy in learning, followed by a comprehensive review of FL schemes and cryptographic privacy-preserving techniques. Then, we present ideas on the emerging area of privacy-preserving optimization, ranging from privacy-preserving distributed optimization to privacy-preserving evolutionary optimization and privacy-preserving Bayesian optimization (BO). We further provide a thorough security analysis of BO and evolutionary optimization methods from the perspective of inferring attacks and active attacks. On the basis of the above, an in-depth discussion is given to analyze what FL and distributed optimization strategies can be used for the design of federated optimization and what additional requirements are needed for achieving these strategies. Finally, we conclude the survey by outlining open questions and remaining challenges in federated data-driven optimization. We hope this survey can provide insights into the relationship between FL and federated optimization and will promote research interest in secure federated optimization.
- Published
- 2024
- Full Text
- View/download PDF
197. Adaptive normal vector guided evolutionary multi- and many-objective optimization
- Author
-
Yicun Hua, Qiqi Liu, and Kuangrong Hao
- Subjects
Polyester fiber ,Evolutionary multi-objective optimization ,Irregular Pareto front ,Reference vector ,Hyperplane ,Clustering ,Electronic computers. Computer science ,QA75.5-76.95 ,Information technology ,T58.5-58.64 - Abstract
Abstract Most existing multi-objective evolutionary algorithms relying on fixed reference vectors originating from an ideal or a nadir point may fail to perform well on multi- and many-objective optimization problems with various convexity or shapes of Pareto fronts. A possible reason could be the inaccurate measurement of the diversity of solutions or the failure of the fixed reference vectors in guiding the rapidly changing population. To meet this challenge, this work develops an adaptive normal reference vector-based decomposition strategy for guiding the search process, which is able to handle various convexity and shapes of Pareto fronts. Specifically, the normal vector passing through the center of each cluster in a constructed hyperplane is adopted as the reference vector for guiding the search process. Then, a selection strategy is put forward based on the positions of solutions in the current population and the normal vectors for the environmental selection. Based on the adaptive normal vectors, the proposed algorithm can not only rapidly adapt to the changing population but also alleviate the influence of the convexity of Pareto fronts on the measurement of diversity. Experimental results show that the proposed algorithm performs consistently well on various types of multi-/many-objective problems having regular or irregular Pareto fronts. In addition, the proposed algorithm is shown to perform well in the optimization of the polyester fiber esterification process.
- Published
- 2024
- Full Text
- View/download PDF
198. Comparative analysis of phosphoproteomic in the intestine of Sepia lycidas under different salinity environments.
- Author
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Changgeng Yang, Qiqi Li, Huimei Chen, Mengyu Yang, Xiang Jiang, and Lele Xu
- Subjects
- *
CUTTLEFISH , *EFFECT of salt on fishes , *PROTEOMICS , *FISH growth , *FISH immunology - Abstract
Cuttlefish are sensitive to the breeding environment, and the low-salinity environment significantly impacts their growth and immunity. So far, it is difficult to breed this species artificially. This study was conducted in Sepia lycidas. And the aim was to investigate the differences in protein phosphorylation in the intestine of S. lycidas under different salinity conditions. Firstly, 999 phosphoproteins (specific peptide = 1), 1928 phosphopeptides, and 2727 phosphorylation sites were identified. Among them were 284 down-regulated expression phosphorylation sites (corresponding to 115 phosphoproteins) and 674 up-regulated expression phosphorylation sites (corresponding to 408 phosphoproteins) in the intestine under a low salinity environment compared with that under a natural salinity environment. Next, GO analysis found that more phosphoproteins corresponding to differentially expressed phosphorylation sites were related to anatomical structure development, multicellular organism development, regulation of the cellular process, etc. The molecular functions of these proteins mainly contain protein binding, transferase activity, catalytic activity, and heterocyclic compound binding. And they are mainly involved in the cellular components of intracellular anatomical structure, organelle, and cytoplasm. KEGG enrichment analysis of the differential phosphoproteins suggested that many significantly enriched pathways were related to the phosphatidylinositol signaling system, cell junction (adherens junction and tight junction), and inositol phosphate metabolism. Finally, changes in environmental salinity can affect the intestinal structure, metabolism, and immune homeostasis of S. lycidas. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
199. Morning glory-inspired lattice structure with negative Poisson's ratio effect
- Author
-
Qiqi Li, Liuyu Zhan, Xiujuan Miao, Lin Hu, Eric Li, and Tiefang Zou
- Subjects
Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Condensed Matter Physics ,Civil and Structural Engineering - Published
- 2022
200. High-speed particle self-rotation in different pre-self-rotation cyclones and application in enhancement of oil recovery from spent catalysts
- Author
-
Tingting Cheng, Qiqi Li, Hualin Wang, Xiangchen Fang, Liquan Li, Lai Jiang, Jianping Li, Yuan Huang, Tao Yang, and Pengbo Fu
- Subjects
Filtration and Separation ,Analytical Chemistry - Published
- 2022
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