151. The long non-coding RNA SNHG1 promotes glioma progression by competitively binding to miR-194 to regulate PHLDA1 expression
- Author
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Hua Chen, Qianqian Jiang, Haiyang Wang, Liang Liu, Jun Dong, Yan Shi, Xiaojian Li, Yujing Sheng, and Jia Shi
- Subjects
0301 basic medicine ,Cancer Research ,Cell biology ,Carcinogenesis ,Molecular biology ,Immunology ,Transplantation, Heterologous ,Mice, Nude ,Apoptosis ,Biology ,Article ,law.invention ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Mice ,0302 clinical medicine ,law ,Cell Movement ,Glioma ,Cell Line, Tumor ,medicine ,Animals ,Humans ,lcsh:QH573-671 ,Small nucleolar RNA ,Cell Proliferation ,Neovascularization, Pathologic ,lcsh:Cytology ,RNA ,medicine.disease ,In vitro ,Long non-coding RNA ,Up-Regulation ,Pleckstrin homology domain ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Glucose ,Cell culture ,030220 oncology & carcinogenesis ,Disease Progression ,Suppressor ,Female ,RNA, Long Noncoding ,Transcription Factors - Abstract
Long non-coding RNAs (lncRNAs) play a vital role in tumourigenesis, including that of glioma. Small nucleolar RNA host gene 1 (SNHG1) is a relatively novel lncRNA that is involved in the development of multiple human tumours. However, its underlying molecular mechanism in glioma has not been completely clarified. In this study, we show that SNHG1 is overexpressed in glioma tissues and cell lines. A series of functional assays suggested that SNHG1 promotes glioma progression in vitro and in vivo. Next, through online databases, a luciferase reporter assay and an RNA pull-down assay, we confirmed that SNHG1 functions as a sponge for miR-194, which acts as a suppressor in glioma. We also verified that pleckstrin homology like domain family A, member 1 (PHLDA1) is the functional target of miR-194. Moreover, rescue experiments demonstrated that SNHG1 regulates PHLDA1 expression in a miR-194-dependent manner. Taken together, our study shows that SNHG1 promotes glioma progression by competitively binding to miR-194 to regulate PHLDA1 expression, which may provide a novel therapeutic strategy for glioma.
- Published
- 2019