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The chemical compositions, phases and structures of two oxide films on Alloy 690TT following exposure for 4400 h in pure water with and without lead at 320 °C were studied by surface analysis techniques. The analysis of a lead-doped oxide film prepared by a focused ion beam (FIB) demonstrated that both Cr-rich and Ni-rich oxides were alternatively distributed within the outer layer, whereas the inner layer was porous and poorly protected, causing severe corrosion of the alloy and a thicker film was formed. A duplex film model was proposed for the effects discussion of lead on the oxidation mechanism.

This study investigated the PbSCC of Alloy 690TT exposed to a caustic solution at 320 °C. Characterization of the resulting films indicates that lead is involved in the formation of oxides and evenly distributed in the films. TEM analysis was carried out to elucidate the role of lead in IGSCC by investigating the oxides at the crack tip. Lead penetrates into the crack tip and disperses in the intergranular oxides, enhancing the oxidation of grain boundaries. Thus, microcracks initiate within oxides under stress and connect with the main SCC crack stemming from the pit, resulting in propagation of the IGSCC.

Thiazole orange (TO) stains most nucleic acids giving strong fluorescence signal non-specifically. To resolve the poor discriminating ability of TO among tertiary structures of DNAs, a planar and rotatable π–conjugated small molecule designed by replacing the methylene bridge with an ethylene that slightly increases its structural flexibility, while maintain the crucial conjugated co-planar two-ring system. The study addressed the origin of signal discrimination for the molecule upon binding with different DNA structures and the results demonstrate that the molecule exhibits excellent fluorescence signal discrimination between the DNA G-quadruplexes and other DNA tertiary structures. In a typical staining assay, the molecule induced 30-fold greater fluorescence signal upon binding with Pu27 than that of ssDNA and dsDNA. Molecular modelling studies also support that the flexible ethylene bridge between the two-ring system is the crucial factor for inducing selectivity towards G-quadruplexes. Furthermore, the selectivity, sensitivity, and stability of the molecule were investigated with various techniques. The application of the molecule as a selective staining agent for imaging of G-quadruplex in human prostate cancer cells PC3 and mouse fibroblast L929 was also investigated. The strongly observed green fluorescence signal in the cell nucleus suggests that the molecule is able to enter into the nucleus and then binding with G-quadruplexes.

A multigroup model is developed to characterize brucellosis transmission, to explore potential effects of key factors, and to prioritize control measures. The global threshold dynamics are completely characterized by theory of asymptotic autonomous systems and Lyapunov direct method. We then formulate a multi-objective optimization problem and, by the weighted sum method, transform it into a scalar optimization problem on minimizing the total cost for control. The existence of optimal control and its characterization are well established by Pontryagin's Maximum Principle. We further parameterize the model and compute optimal control strategy for Inner Mongolia in China. In particular, we expound the effects of sheep recruitment, vaccination of sheep, culling of infected sheep, and health education of human on the dynamics and control of brucellosis. This study indicates that current control measures in Inner Mongolia are not working well and Brucellosis will continue to increase. The main finding here supports opposing unregulated sheep breeding and suggests vaccination and health education as the preferred necessary emergency intervention control. The policymakers must take a new look at the current control strategy, and, in order to control brucellosis better in Inner Mongolia, the governments have to preemptively press ahead with more effective measures.

The latent period is one of the important risk factors considered in epidemiological research literatures. In general, a latent period can be modelled by incorporating a delay effect (delay system), or by introducing an exposed class defined as E. In this paper, a susceptible-vaccinated-exposed-infectious-pathogen (SVEIP) dynamic model and its corresponding delayed SVIP model are proposed. Under biologically motivated assumptions, the stability of equilibria is investigated by the global Lyapunov functions and functionals, and the dynamical properties of two systems are found to depend entirely on the basic reproduction numbers R 0 1 and R 0 2 : if R 0 1 ( R 0 2 ) ≤ 1 , the disease-free equilibrium is globally asymptotically stable; if R 0 1 ( R 0 2 ) > 1 , the endemic equilibrium exists and is globally asymptotically stable, which implies time delay span has no effect on the stability of equilibria in delay system. Finally, a comparison between SVEIP and delayed SVIP epidemic model is made by numerical analysis, elaborating the epidemiological significance of these results.

Background Esophageal squamous cell carcinoma (ESCC) cells are heterogeneous, easily develop radioresistance, and recur. Single-cell RNA-seq (scRNA-seq) is a next-generation sequencing method that can delineate diverse gene expression profiles of individual cells and mining their heterogeneous behaviors in response to irradiation. Our aim was using scRNA-seq to describe the difference between parental cells and cells that acquired radioresistance, and to investigate the dynamic changes of the transcriptome of cells in response to FIR. Results We sequenced ESCC cell lines KYSE180 with and without fractionated irradiation (FIR). A total of 218 scRNA-seq libraries were obtained from 88 cells exposed to 12 Gy (KYSE-180-12 Gy), 89 exposed to 30 Gy (KYSE-180-30 Gy), and 41 parental KYSE-180 cells not exposed to FIR. Dynamic gene expression patterns were determined by comprehensive consideration of genes and pathways. Biological experiments showed that KYSE-180 cells became radioresistant after FIR. PCA analysis of scRNA-seq data showed KYSE-180, KYSE-180-12 Gy and KYSE-180-30 Gy cells were discrete away from each other. Two sub-populations found in KYSE-180-12 Gy and only one remained in KYSE-180-30 Gy. This sub-population genes exposure to FIR through 12 Gy to 30 Gy were relevant to the PI3K-AKT pathway, pathways evading apoptosis, tumor cell migration, metastasis, or invasion pathways, and cell differentiation and proliferation pathways. We validated DEGs, such as CFLAR, LAMA5, ITGA6, ITGB4, and SDC4 genes, in these five pathways as radioresistant genes in bulk cell RNA-seq data from ESCC tissue of a ESCC patient treated with radiotherapy and from KYSE-150 cell lines. Conclusions Our results delineated the divergent gene expression patterns of individual ESCC cells exposure to FIR, and displayed genes and pathways related to development of radioresistance. Electronic supplementary material The online version of this article (10.1186/s12864-019-5970-0) contains supplementary material, which is available to authorized users.

This paper focus on video recommendation in response to the explosive growth of movies which has made people in the dilemma of information overload for a long time. It is particularly important to push movies accurately. Regarding this situation, this paper studies how to recommend the most similar movies to users based on their interest tags and browsing behavior. Compared with the collaborative filtering algorithm, whose processing effect is not good confronting scoring data sparse problem. This paper makes full use of the movie review data, and the experimental data set is obtained by crawling the high-quality review of the Douban website. The experimental results show that the proposed method can effectively solve the problem of difficulty in calculating the similarity of the traditional recommendation algorithm when the user data set is incomplete, and the recommended effect is satisfactory.

In recent years, synergy theory has made great progress in the field of power electronics, and its good robustness provides a new idea for motor control research. In this paper, we take use of rapid convergence of manifold, and introduce it into the control of the speed and flux linkage of the traditional deadbeat control (DC stands for deadbeat control and IDC stands for improved deadbeat control), aiming to eliminate the overshoot problem in the traditional DC. Based on the above work, this paper deduces the improved control algorithm based on synergy theory for deadbeat prediction. The simulink simulation platform is built. The feasibility of the theory is verified by algorithm and simulation analysis.

Intratumoral heterogeneity, particularly the potential cancer stemness of single cancer cells, has not yet been fully elucidated in human esophageal cancer. Single-cell transcriptome sequencing of two types of esophageal adenocarcinoma (EAC) and two types of esophageal squamous cell carcinoma (ESCC) tissues was performed, and the intratumoral cancer stemness of the types of esophageal cancer were characterized at the single-cell level in the present study. By comparing the transcriptomic profiles of single cancer cells with high and low stemness in individual patients, it was revealed that the overexpression of cell cycle-associated genes in EAC cells was highly correlated with stemness, whereas overexpression of genes involved in the signaling pathways of DNA replication and DNA damage repair was significantly correlated with stemness in ESCC. High expression of these stemness-associated genes was correlated with poor prognosis of patients. Additionally, poly [ADP-ribose] polymerase(PARP)4 was identified as a novel cancer stemness-associated gene in ESCC and its association with survival was validated in a cohort of 121 patients with ESCC. These findings have profound potential implications for the use of cell cycle inhibitors in EAC and PARP inhibitors in ESCC, which may provide novel mechanistic insights into the plasticity of esophageal cancer.

In order to improve the heat management level and monitoring quality of urban heat network, a genetic algorithm based PID hot network monitoring system is designed. The system uses PLC as the controller and uses the analog input module to collect the outdoor temperature in real time. According to the average temperature curve of supply and return of the secondary pipe network, the genetic algorithm is used to optimize the PID parameters online and control the average temperature of the secondary pipe network supply and return water. The simulation results show that the PID based on genetic algorithm has better overshooting, faster control time and stronger robustness than traditional PID control, and has good application value.

Background Dysregulation of numerous lncRNAs has been recently confirmed in glioma; however, the majority of their roles and mechanisms involved in this notorious disease remain largely unclear. This study aims to explore the roles and molecular mechanisms of LINC01198 implicated in the proliferation and chemoresistance in glioma. Results LINC01198 was elevated in glioma, and this predicted a poorer prognosis for patients with glioma. LINC01198 knockdown inhibited, while LINC01198 overexpression promoted, glioma cell proliferation and resistance to temozolomide. Mechanistically, NEDD4-1 (neural precursor cell expressed, developmentally downregulated 4, E3 ubiquitin protein ligase) and phosphatase and tensin homolog (PTEN) were recruited by LINC01198, which functioned as a scaffold. Moreover, we showed that LINC01198 exerted its oncogenic activities by enhancing the NEDD4-1-dependent repression of PTEN. Conclusions Our study elucidated the role of oncogenic LINC01198 in glioma proliferation and temozolomide resistance, and this role may serve as a promising target for glioma therapy. Methods LINC01198 expression in glioma tissues and that in paired normal tissues were measured by qRT-PCR. The functional roles of LINC01198 in glioma were demonstrated by a series of in vitro experiments. CCK-8 assay, RNA pulldown, RNA immunoprecipitation and western blotting were used to demonstrate the potential mechanisms of LINC01198.

The supplier is considered to be attractive under green supply chain management; supplier evaluation is also confronted with a serious challenge. Based on this background, this paper studies green supplier evaluation and selection with weights unknown in hesitant fuzzy sets and presents evaluation indexes by the DEMATEL method. On the basis of the Euclidean distance, hesitancy degree is used to construct the improved type signed distance. At last, the paper calculates relative closeness to rank green suppliers by the improved TOPSIS method and compares the results of the traditional algorithm and SAW method. The result of the example shows that the algorithm has practicability and a dipartite degree.

Anterior segment dysgenesis is often associated with cornea diseases, cataracts and glaucoma. The ciliary body (CB) is a part of the anterior segment that secretes aqueous and vitreous humor that nourishes the eye. Although defective Notch signaling in the CB disrupts CB morphogenesis and causes eye degeneration, the underlying mechanisms remain missing. Here, this study shows that NOTCH signaling controls CB morphogenesis by directly regulating Nectin1 expression, and maintains the vitreous and eye structures by regulating the expression of vitreous proteins and gap junction protein Cx43. Genetic inactivation of Rbpj or both Notch2 and Notch3 in the CB not only disrupts CB morphogenesis but also causes CB epithelial detachment and vitreous shrinkage in the developing eye, and eye degeneration in adults. Molecularly, NOTCH signaling transcriptionally controls the expression of adhesion protein Nectin1 known to be essential for CB morphogenesis, and controls eye pressure and the vitreous through stabilizing gap junction protein Cx43 known to be important for CB secretion. Mechanistically, Nectin1 directly binds and stabilizes Cx43. Finally, NOTCH signaling in the CB also directly controls the expression of vitreous proteins. Therefore, this study has provided important mechanistic insight into how NOTCH signaling controls CB morphogenesis and secretion, and also further suggests the potential involvement of CB secretion in various eye diseases, including retinal degeneration.

Aggregation is driven by both the size of the concentration and the city itself in the industry (urbanization). Since the new company to follow the existing companies in the same industry continues to cluster in these areas, the localized economy will create a cluster to produce the same product. The crisis of power shortage in the telecommunications industry in the summer of the peak power consumption period reduces the power cost through load management measures based on Neural Network (NN) using FPGA tool. Field of Business Intelligence (BI) is, by performing the direct mining data in the database, applying the power of data mining in a number of the hands of the business line and analysts. Sample of the customer, install the collect energy consumption data that the digital power meter. Effective load management strategies have been proposed; the load management measures’ contents can be summarized to compare the measured data. Result join the show's load management strategy, the telecommunications industry, and are very satisfied. In the margin adjustment and Time of Use (ToU) period, customers of this industry can save a certain amount of electricity.

The healing of burn wounds has been widely characterized to be highly intricate, involving processes such as neo-vascularization, granulation, re-epithelialization, inflammation and wound contraction. Various therapies are available for the management of burn wounds; however, a truly effective therapeutic strategy has yet to be identified due to safety issues. The aim of the present study was to assess and confirm the burn wound healing properties of the compounds asiaticoside (AE) and madecassoside (MA), which are found in the herb Centella asiatica. The cytotoxic nature of the AE and MA were inspected and were confirmed to be non-toxic up to 500 ppm. The compounds AE and MA increased monocyte chemoattractant protein-1 production, but caused no significant effect on vascular endothelial growth factor production. In addition, an in vivo animal burn model was employed to represent the features of burn wound healing. Hence, the present results warrant the further investigation of C. asiatica extracts for use in burn healing.

The genome of a highly pathogenic strain of Aleutian disease mink virus (AMDV-BJ) isolated from a domestic farm in North China has been determined and compared with other strains. Alignment analysis of the major structural protein VP2 revealed that AMDV-BJ is unique among 17 other AMDV strains. Compared with the nonpathogenic strain ADV-G, the 3' end Y-shaped hairpin was highly conserved, while a 4-base deletion in the 5' U-shaped terminal palindrome resulted in a different unpaired "bubble" group near the NS1-binding region of the 5' end hairpin which may affect replication efficiency in vivo. We also performed a protein analysis of the NS1, NS2, and new-confirmed NS3 of AMDV-BJ with some related AMDV DNA sequence published, providing information on evolution of AMDV genes. This study shows a useful method to obtain the full-length genome of AMDV and some other parvoviruses.

A virus isolated from mink showing clinical signs of enteritis was identified as a high virulent mink enteritis parvovirus (MEV) based on its biological characteristics in vivo and in vitro. Mink, challenged with this strain named MEV-LHV, exhibited severe pathological lesions as compared to those challenged with attenuated strain MEV-L. MEV-LHV also showed higher infection and replication efficiencies in vitro than MEV-L. Sequence of the complete genome of MEV-LHV was determined and analyzed in comparison with those in GenBank, which revealed that MEV-LHV shared high homology with virulent strain MEV SD12/01, whereas MEV-L was closely related to Abashiri and vaccine strain MEVB, and belonged to a different branch of the phylogenetic tree. The genomes of the two strains differed by insertions and deletions in their palindromic termini and specific unique mutations (especially VP2 300) in coding sequences which may be involved in viral replication and pathogenicity. The results of this study provide a better understanding of the biological and genomic characteristics of MEV and identify certain regions and sites that may be involved in viral replication and pathogenicity.

Circulating tumor DNA (ctDNA) is becoming an important biomarker in noninvasive diagnosis and monitoring of tumor dynamics. This study tested the feasibility of plasma ctDNA for the non-invasive analysis of tumor mutations in esophageal squamous cell carcinoma (ESCC) by sequencing of tumor, tumor-adjacent, and normal tissue, as well as pre-surgery and post-surgery plasma. Exome sequencing of eight patients identified between 29 and 134 somatic mutations in ESCCs, many of which were also determined in ctDNA. Comparison of pre-surgery and post-surgery plasma has shown that mutations had reduced frequency or disappeared after surgery treatment. We further evaluated the TruSight Cancer sequencing panel by using it to detect mutations in the plasma of three patients. Tumor mutations were only found in one of them. To design a sequencing panel with improved targeting, we identified significantly mutated genes by meta-analysis of 532 ESCC genomes. Our results confirmed the well-known driver genes and found several uncharacterized genes. The new panel consisted of 90 recurrent genes, which theoretically achieved 94% and 75% of sensitivity when detecting at least 1 and 2 mutant genes in ESCC patients, respectively. Our results demonstrate the feasibility of using ctDNA to detect ESCCs and monitor treatment effect. The low-cost and sensitive target panel could facilitate clinical usage of ctDNA as a noninvasive biomarker.

For the prevention and control of brucellosis, it is important to investigate the mechanism of brucellosis transmission. Based on the charac- teristics of the spread of brucellosis, a susceptible-exposed-infectious-brucella (SEIB) delay dynamic model is proposed with the general incidence, elim- ination rate and shedding rate of pathogen. Under biologically motivated assumptions, it shows the uniqueness of the endemic equilibrium, and inves- tigates the global asymptotically stability of the disease-free equilibrium and the endemic equilibrium. The results suggest that the global stability of equi- libria depends entirely on the basic reproduction number R0 and time delay is harmless for the stability of equilibria. Finally, some specic examples and numerical simulations are used to illustrate the utilization of research results and reveal the biological signicance of hypothesis ( H7), which implies that the dynamics of brucellosis transmission depend largely on the development of the prevention and control strategies.

The transmission mechanism of some animal diseases is complex because of the multiple transmission pathways and multiple-group interactions, which lead to the limited understanding of the dynamics of these diseases transmission. In this paper, a delay multi-group dynamic model is proposed in which time delay is caused by the latency of infection. Under the biologically motivated assumptions, the basic reproduction number R0 is derived and then the global stability of the disease-free equilibrium and the endemic equilibrium is analyzed by Lyapunov functionals and a graph-theoretic approach as for time delay. The results show the global properties of equilibria only depend on the basic reproductive number R0:the disease-free equilibrium is globally asymptotically stable if R0 ≤ 1; if R0>1, the endemic equilibrium exists and is globally asymptotically stable, which implies time delay span has no effect on the stability of equilibria. Finally, some specific examples are taken to illustrate the utilization of the results and then numerical simulations are used for further discussion. The numerical results show time delay model may experience periodic oscillation behaviors, implying that the spread of animal diseases depends largely on the prevention and control strategies of all sub-populations.

In this paper, V2O5 was added into vitrified bond as a surfactant to improve the holding force of the vitrified bond to diamond abrasive and to improve the grinding performance of vitrified diamond grinding wheel. Results showed that the refractoriness of the vitrified bond with 4 wt% V2O5 reached 555 °C, 17 °C lower than that of the vitrified bond without V2O5. During sintering, vanadium ions in the bond was enriched on the diamond surface and combined with the unsaturated carbon atom existed on the surface of diamond to form V C bond, which can improve the wettability between vitrified bond and diamond abrasive; Li2Si2O5 phase began to precipitate in bond when addition of V2O5 content reached 2 wt%; The Flexural strength and Rockwell hardness were significantly improved from 83.7 MPa and 87.4 HRB to 112.6 MPa and 108.1 HRB, 34.5% and 23.7% higher than those of diamond composites without V2O5, respectively. The performance of the diamond grinding wheel prepared by adding 2 wt% V2O5 to the vitrified bond was improved, The roundness and straightness errors of the 50th zirconia workpiece in one dressing cycle decreased from 3.31 and 2.11 μm to 3.02 and 1.9 μm, respectively, as compared with the vitrified diamond wheel without V2O5.

The freestanding of reduced graphene oxides/carbon nanotubes (rGO/CNTs) hybrid films are synthesized via the simple vacuum filtration and thermal reduction methods. And the electrochemical behaviors of rGO/CNTs hybrid films are investigated in KOH and in Et4NBF4/AN electrolyte, respectively. In three-electrode systems, the rGO/CNTs hybrid films show a maximum specific capacitance of 221 F g−1, a 71% capacitance retention, and an excellent cycle life in 1 M KOH electrolyte. And the electrochemical behaviors of rGO/CNTs films in Et4NBF4/AN electrolyte under three-electrode systems show a maximum specific capacitance of 174 F g−1 and good rate capability. Moreover, a symmetric supercapacitor of rGO/CNTs//rGO/CNTs demonstrates a maximum specific capacitance of 24 F g−1 at 1 A g−1, a energy density of 20.8 Wh kg−1 at 1.27 kW kg−1, and an excellent cycle life of 86.1% retention after 5000 cycles. It suggests that the symmetric supercapacitor could be regarded as an ideal energy storage system.

To improve the ineluctable agglomeration and weak inherent conductivity of MgAl

Under the background of engineering education professional certification, this article puts forward the construction of new training model for automation professionals. This model aims at cultivating engineering innovation and entrepreneurial talents. Through a series of measures such as training program optimization, curriculum system renewal, teaching method reform and practical teaching enhancement, it has realized the cultivation of professional talents with high quality, strong ability and quick adaptability. The practice shows that the new talent training model can effectively improve students' comprehensive quality, practical and innovative abilities, and employment competitiveness.

Background Previous real-world comparative research of MS disease modifying therapies (DMTs) in the overall population has suggested dimethyl fumarate (DMF) to be comparable to fingolimod (FTY) and more efficacious than teriflunomide (TERI) in reducing relapses. However, there is limited comparative evidence in patients switching from platform DMTs in the US. The objective of the study was to compare the annualized relapse rate (ARR) and risk of relapse in MS patients who have switched from a platform therapy to DMF, FTY, or TERI. Methods MS patients (18–65 years old) initiating an oral DMT from June 2013 to March 2015 were identified from the Truven MarketScan® Commercial Claims Database. The index date was the date of first oral DMT fill. Patients were required to have: continuous enrollment in the database for 12 months pre-index date and ≥3 months post-index date; ≥1 MS diagnosis over the pre-index period; discontinuation of a platform DMT with no evidence of oral or infusion DMTs over the pre-index period; and adherence to the index drug for ≥90 days. DMF patients were propensity-score matched (PSM) 3:1 to FTY and to TERI based on age, gender, region, a claims-based MS severity measure, ARR, and number of hospitalizations over the pre-index period. Patients were censored when they dropped out of the database or at the end of the study period (March 31, 2016). Post-index relapses were annualized. Results The database included 20,311 oral DMT users. After applying the study criteria, the PSM yielded 1602:534 switch patients for the DMF–FTY matched cohort. DMF–FTY patients were well-matched on all covariates: age (mean = 44 for both), gender (28% vs. 26% male, respectively), MS severity measure (0.99 vs. 1.08), and baseline ARR (0.40 vs. 0.44). PSM yielded 833:279 switch patients for the DMF–TERI match. DMF–TERI patients were well-matched on all covariates: age (mean = 50), gender (24% vs. 25% male), MS severity measure (0.86 vs. 0.99), and baseline ARR (0.23 vs. 0.30). The standardized differences confirmed balance across all covariates for matched cohorts. The matched DMF–FTY cohorts had comparable post-index ARR (Rate Ratio [RR] = 1.07 [95% Cl: 0.861, 1.328]) and risk of relapse (Hazard Ratio [HR ]= 0.996 [95% CI: 0.803, 1.236]). Post-index ARR was significantly lower with DMF in comparison to TERI (RR = 0.667 [0.486, 0.914]). The risk of relapse was also significantly lower when switching to DMF than TERI (HR = 0.679 [0.503, 0.917]). Conclusion In this analysis, the effectiveness profiles for those oral DMT users specifically switching from platform therapies are consistent with findings from previous research conducted among all oral DMT users, regardless of prior therapy.

The present study investigated the effects of nicorandil on cerebral injury following cardiopulmonary resuscitation (CPR) in a swine model of cardiac arrest. CPR was performed on swine following 4 min induced ventricular fibrillation. Surviving animals were randomly divided into 3 groups: A nicorandil group (n=8), a control group (n=8) and a sham group (n=4). The sham group underwent the same surgical procedure to imitate cardiac arrest, but ventricular fibrillation was not induced. When the earliest observable return of spontaneous circulation (ROSC) was detected, the nicorandil and control groups received injections of nicorandil and saline, respectively. Swine serum was collected at baseline and 5 min, 0.5, 3 and 6 h following ROSC. Serum levels of neuron-specific enolase (NSE), S100β, tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) were measured using ELISA. Animals were euthanized and brain tissue samples were collected and assessed using light and electron microscopy 6 h following ROSC. The expression of aquaporin-4 (AQP-4) in the brain tissue was measured using western blotting. Malondialdehyde (MDA) and glutathione (GSH) levels in the brain tissue were determined using thiobarbituric acid and thiobenzoic acid colorimetric methods, respectively. Serum NSE and S100β were significantly higher in the nicorandil and control groups following CPR, compared with baseline (P

A symmetric ligand is synthesized composed of a core

Psoriasis is an autoimmune disease involving the excessive proliferation of keratinocytes mediated by T-cells. Parathyroid hormone (PTH) has been identified as an essential factor in the treatment of psoriasis. In the present study, the mechanism underlying the effect of recombinant human parathyroid hormone (rhPTH) (1-34) in keratinocytes was investigated. The effects of rhPTH (1-34) on cell proliferation, cell cycle, and the secretion and expression of C-X-C motif chemokine 11 (CXCL11) and components of the Hedgehog signaling pathway were examined in HaCaT cells by MTT assay, flow cytometric analysis, ELISA and gene chip analysis. The data showed that rhPTH (1-34) significantly inhibited keratinocyte proliferation at concentrations >8×10−7 mol/l. rhPTH (1-34) induced G1 phase arrest of the cell cycle in the keratinocytes. The secretion of CXCL11 in tumor necrosis factor (TNF)-α-induced keratinocytes was downregulated by rhPTH (1-34) in a dose-dependent manner, compared with that in keratinocytes treated with TNF-α alone. It was also found that rhPTH (1-34) inhibited the expression of CXCL11 in the HaCaT cells. rhPTH (1-34) also affected the Hedgehog signaling pathway specifically by regulating the expression of associated genes. In conclusion, these data suggested that rhPTH (1-34) inhibited cell proliferation, and the secretion and expression of CXCL11 in HaCaTs. rhPTH (1-34) also altered the expression of associated genes in the Hedgehog pathway. Therefore, rhPTH (1-34) can be considered as a novel therapeutic agent for the treatment of psoriasis.

Tumor heterogeneity and internal clonal evolution of human colorectal cancer stem cells (CRCSCs) within cancerous tissues often represents an aggressive feature of cancer progression. For a high-resolution examination of CRCSCs, we performed single cell whole-exome sequencing (scWES) of sorted cells, including three groups of CRCSCs: CD133+CD44+, CD133-CD44+, or CD133+CD44- cells, colorectal cancer cells (CRCCs) lacking these stem-cell markers (EpCAM+), plus one group of normal control cells. We examined and validated the scWES data for stem-specifically somatic alterations, clonal evolution, etc. Clonal evolution analysis showed that all CRCSCs were located at the main clone, and that the CD133+CD44- CRCSCs, which harbored the lowest number of mutations, were the likely origin of colorectal cancer (CRC) before evolving into other groups of CRCSCs/CRCCs. These results were validated by cell-lines culture experiments. Functional analyses validated that stem cell-specific mutated genes, such as GOLGA8J, ARHGAP39, LURAP1L, CDH1, were positively associated with stemness and knocking down each of them could increase the proportion of CD133+ cells. To validating mutations, especially stem cell-related genes, we performed targeted exome sequencing using a panel of all mutated genes to analyze the distribution of them in 96 samples (20 patients) those were also sorted into the same three groups of CRCSCs and CRCCs. We found two mutational sites of AHNAK2 gene (Chr14_105410531 and Chr14_105410533) were stem-cell specific in both scWES data and target sequencing data. PLIN4, HLA-B, ALK, CCDC92 and ALMS1 genes were specifically mutated in stem cells in both data. Another 33 genes tended to mutate in stem cells from all data. In all stem cell-related genes, AHNAK2, PTPRF and NIPPA5 which subcellularly located at plasma membrane had totally eleven predicted neoantigens. These neoantigens might be useful for some CRC patients in immunotherapy. All integrative analyses above suggested new markers for CRCSCs and showed the important roles of CRCSCs in tumorigenesis and progression of CRCs.

AlY coating on 1Cr18Ni9Ti stainless steel was prepared by magnetron sputtering method and some of them were pre-oxidized or vacuum diffusion annealed at 600°C, and the effect of the coating with different treatments on the oxidation behavior of the stainless steel was studied at 1,100°C in air. Results show that the order of the 24-h oxidation mass gain for the specimens is the stainless steel without coating > the stainless steel with coating but without any pre-treatment > the stainless steel with AlY coating after pre-oxidation treatment > the stainless steel with AlY coating after vacuum diffusion annealing. After oxidation, a thick and loose Fe2O3/Cr2O3 film is formed on the stainless steel without coating, while thinner Fe2O3/Cr2O3 film is formed on the stainless steel with AlY coating. Compared to the oxidation film formed on the steel with pre-oxidized coating, the one formed on the steel with vacuum diffusion annealed coating is thinner and denser. The rare earth Y and its oxides Y2O3 in the coating produce reactive element effect and improve the ductility/adhesion of the oxide film, which enhances the oxidation resistance of the stainless steel, especially in the vacuum diffusion annealed AlY coating.

Pancreatic cancer (PCC) is one of the most difficult cancers to treat and the 10th leading cause of cancer-related death in worldwide. Studies have demonstrated that the tetraspanin 1 (Tspan1) is overexpressed in various cancers and may be a potential therapeutic strategy for the treatment of different cancers. However, the possible role of Tspan1 in PCC is still unknown. In the present study, our data revealed that the increased Tspan1 in PCC tissues was associated with the clinicopathological features and survival rate of PCC patient. We also investigated the effects of Tspan1 gene knockdown on the biological behavior of human PCC. The expression of Tspan1 (detected by immunohistochemistry, qRT-PCR and western blot analysis) derived from human PCC tissues and cell lines (AsPC-1 and PANC-1), were significantly elevated compared with those of the control (P

c-MYC is an important oncogene that is considered as an effective target for anticancer therapy. Regulation of this gene's transcription is one avenue for c-MYC-targeting drug design. Direct binding to a transcription factor and generating the intervention of a transcriptional programme appears to be an effective way to modulate gene transcription. NM23-H2 is a transcription factor for c-MYC and is proven to be related to the secondary structures in the promoter. Here, we first screened our small-molecule library for NM23-H2 binders and then sifted through the inhibitors that could target and interfere with the interaction process between NM23-H2 and the guanine-rich promoter sequence of c-MYC. As a result, a quinazolone derivative, SYSU-ID-01: , showed a significant interference effect towards NM23-H2 binding to the guanine-rich promoter DNA sequence. Further analyses of the compound-protein interaction and the protein-DNA interaction provided insight into the mode of action for SYSU-ID-01: . Cellular evaluation results showed that SYSU-ID-01: could abrogate NM23-H2 binding to the c-MYC promoter, resulting in downregulation of c-MYC transcription and dramatically suppressed HeLa cell growth. These findings provide a new way of c-MYC transcriptional control through interfering with NM23-H2 binding to guanine-rich promoter sequences by small molecules.

Brucellosis is one of the major public health problems in Jilin Province of China, especially in Changling County of Jilin Province where at least 95 % of the human brucellosis cases are caused by sheep, which attribute to the large number of sheep kept there and the high positive rate of sheep. In this paper, based on the monitoring data and the characteristics of brucellosis infection in Changling County of Jilin Province, a multi-stage dynamic model is proposed for sheep brucellosis transmission, involving young sheep population and adult sheep population. Firstly, the basic reproduction number $$R_0$$ is determined and then the dynamic properties of the model is further discussed. By carrying out sensitivity analysis of the basic reproduction number in terms of some parameters, it concluded that the birth rate of sheep, sheep vaccination rate, and the elimination rate of infectious sheep play an important roles in the transmission of brucellosis. After investigating and comparing the effect of vaccination and culling strategies is completed, the results show that vaccinating sheep and culling infectious sheep are two effective and feasible strategies to control the spread of brucellosis in Changling County of Jilin Province, but the latter is more effective than the former.

The study aimed to clarify the relationship between β-elemene, a long noncoding RNA (lncRNA), and human telomerase reverse transcriptase (hTERT) in esophageal carcinoma ECA-109 cells. The proliferation of ECA-109 cells was measured using a CCK-8 kit and flow cytometry. PCR microarray and real-time RT-PCR were designed to determine lncRNA expression in ECA-109 cells before and after treatment with β-elemene. Western blot was used to detect the hTERT level after the differentially expressed lncRNAs in ECA-109 cells were interfered with small interfering RNA (siRNA). On treatment with β-elemene, the proliferation of ECA-109 cells was notably inhibited, and about 85% of the lncRNAs showed higher expression levels in ECA-109 cells than in those untreated cells, from which, CDKN2B-AS1 was screened out. A specific siRNA (si-CDKN2B-AS1) that targets the β-elemene-mediated lncRNA CDKN2B-AS1 was designed, synthesized, and applied to treat ECA-109 cells. Its interference efficiency reached as high as 89.6%. When ECA-109 cells were transfected with the siRNA, the hTERT level was increased by 84.7%. The CCK-8 assay showed that the proliferation of ECA-109 cells treated with β-elemene was significantly promoted after siRNA transfection (P

Cross-efficiency evaluation method is an effective and widespread adopted data envelopment analysis (DEA) method with self-assessment and peer-assessment to evaluate and rank decision making units (DMUs). Extant aggressive, benevolent, and neutral cross-efficiency methods are used to evaluate DMUs with competitive, cooperative, and nontendentious relationships, respectively. In this paper, a symmetric (nonsymmetric) compete-cooperate matrix is introduced into aggressive and benevolent cross-efficiency methods and compete-cooperate cross-efficiency method is proposed to evaluate DMUs with diverse (relative) relationships. Deviation maximization method is applied to determine the final weights of cross-evaluation to enhance the differentiation ability of cross-efficiency evaluation method. Numerical demonstration is provided to illustrate the reasonability and practicability of the proposed method.

Based on the measured water level data after the impound of Three Gorges reservoir, the water level short-term prediction model of income flow of Chenglingji, Han river and Hukou is constructed by multiple regression method. The comparative of measured water level and predicted water level indicated that, the prediction of income flow is accord with the real flow. Meanwhile, according to statistical analysis of the water level and flow, and considering the total inflow and the jacking of branch inflow, the water level short-term prediction model for middle stream Yangtze River is set up separately. Then, by using multiple regression model, the multiple regression formula for water level prediction is constructed , to applied to the river reach where branch inflowed or river reach jacked by the downstream. Compared with the field observation data, the prediction results are quite precisely.

Since the mid-1990s, both domestic and international tourism in China has grown rapidly in many destinations. In particular, city tourism plays an important role as a major destination in regional tourism development. To promote tourism, understanding image and promotion has become the key point in the development of regional tourism economy. Tour cities image advertising videos, the most visual description and most vivid illustration, have been one of the most important means in the images advertising videos of city destinations. Based on the internet, this article takes advertising videos of main tour cities in China as research data, acquires methods of movie reviews, and studies the content and techniques of the photographers. Moreover, this author puts forward suggestions on the images advertising videos of tour cities from the angles of subjects, contents and techniques of shooting.

We have developed an incandescent Mo source to fabricate large-area single-crystalline MoSe

To compare efficacy and safety of minimally invasive sinus tarsal approach versus conventional L-shaped lateral approach in treating calcaneal fractures.The studies concerning about randomized controlled trial and non-randomized controlled trial of minimally invasive sinus tarsal approach versus conventional L-shaped lateral approach in treating calcaneal fractures from the time of creating database to March, 2017 were searched from PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), EMbase, ISI Web of Knowledge databases, VIP, CNKI, CBM and Wan Fang. The literatures which screened by randomized controlled trial and non- randomized controlled trial were extracted and performed quality assessment by two people. Meta analysis were performed by RevMan 5.3 software and GRADE system were used to evaluate quality.Four randomized controlled trial and 4 non-randomized controlled trial were included, totally 493 patients. Meta-analysis results showed compared with conventional L-shaped lateral approach, minimally invasive sinus tarsal approach had shorter operative time [MD=-5.41, 95%CI(-6.71, -4.12),Minimally invasive sinus tarsal approach and conventional L-shaped lateral approach has similar clinical effect for the treatment of calcaneal fractures. However, minimally invasive sinus tarsal approach has advantages of shorter operation time, lower incidence of complication and better safety. For the limited quantity of the original studies, operative approach should be chosen according to the patient.

As a sort of nonlinear shock absorption component, the disk spring can bear large-scale load with smaller deformation. Disk spring have the properties of high energy conversion proficiency and high reliability, stable output, etc. While, conventional disk spring can’t solve these problems very well in their applications. In the paper, a new supporting disk spring of traveler designed and analyzed, will meet the requirements of operating mechanism for new circuit-breaker by analyzing characteristics of the special disk spring.

Curcumin is a potent anti-cancer drug in several types of human cancers. Despite of several preclinical and clinical studies of curcumin, the precise mechanism of curcumin in cancer prevention has remained unclear. In our study, we for the first time investigated whole transcriptome alteration in A549 non–small cell lung cancer (NSCLC) cell lines after treatment with curcumin using RNA sequencing. We found that lots of genes and signaling pathways were significantly altered after curcumin treatment in A549 cells. With bioinformatics approaches (gene ontology, Kyoto Encyclopedia of Genes and Genomes, and STRING), we found that those curcumin altered genes were not only the genes that induce cell death but also those extracellular matrix receptors and mitogen-activated protein kinase signaling pathway genes which regulate cell migration and proliferation. Among those significantly altered genes, eight genes ( COL1A1, COL4A1, COL5A1, LAMA5, ITGA3, ITGA2B, DDIT3 , and DUSP1 ) were further examined by quantitative reverse transcription polymerase chain reaction and western blot analysis in four non–small cell lung cancer cell lines. Both in cell lines and in mouse model, the extracellular matrix receptors including the integrin ( ITGA3 and ITGA2B ), collagen ( COL5A1 ), and laminin ( LAMA5 ) were significantly inhibited by curcumin at messenger RNA and protein levels. Functional studies confirmed that curcumin not only induced A549 cell death but also repressed cell proliferation and migration by regulating extracellular matrix receptors. Collectively, our study suggests that curcumin may be used as a promising drug candidate for intervening lung cancer in future studies.

The discovery of novel anti-AML therapeutic agents is urgently needed, but the complex heterogeneity of the disease has so far hampered the development of a curative treatment. FLT3 inhibitors have shown therapeutic potential in clinical trials; but a monotherapy regimen has been associated with resistance mediated by the activation of parallel signalling circuitry, including MAPK and mTOR. Therefore, inhibiting a nexus of the two signalling pathways along with inhibition of FLT3 might be advantageous. Herein, we propose that a dual inhibition of FLT3 and Mnk would provide a better clinical option for AML patients compared to targeting FLT3 alone. Thus, a series of N-phenyl-4-(thiazol-5-yl)pyrimidin-2-amines and 4-(indol-3-yl)-N-phenylpyrimidin-2-amines were prepared. Potent Mnk2 inhibitors, FLT3 inhibitors, and dual inhibitors of Mnk2 and FLT3 were identified and their anti-proliferative activities assessed against MV4-11 AML cell lines. Dual inhibition of FLT3 and Mnk2 caused the increased apoptotic cell death of MV4-11 cells compared to inhibition of FLT3 or Mnk2 alone. Refereed/Peer-reviewed

Based on the summarizing of previous research results, a two-dimensional mathematical model of average distribution along water depth is developed for the research on controlling conditions of cooling water from Small Modular Reactors at coastal site. This paper proposed two kinds of boundary conditions: straight shoreline and concave type bay. The result shows that for straight shoreline, the average depth of 5 m can be used as a control condition of cooling water for flat seabed and the slope of 0.4 m/km can be used as a control condition of cooling water for sloping seabed. The result also shows that for concave type bay, the average depth of 6 m can be used as a control condition of cooling water for flat seabed and the slope of 0.53 m/km can be used as a control condition of cooling water for sloping seabed. Besides, is that the near-zone water really participates in the mixed dilution and diffusion of warm water rather than the tidal prism plays a decisive role on the corresponding water area as the bay is large enough. The research results may serve as reference for the determination of fitness standards about Small Modular Reactors at coastal site.

This study sought to evaluate the diagnostic value of the methylation of multiple gene promoters in serum in hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC). A total of 343 participants were enrolled, including 98 patients with HCC, 75 patients with liver cirrhosis (LC), 90 patients with chronic hepatitis B (CHB), and 80 healthy individuals. RASSF1A, APC, BVES, TIMP3, GSTP1, and HOXA9 were selected as the candidate genes. The MethyLight method was used to assay promoter methylation statuses. The diagnostic performances of markers were assessed by constructing receiver operating characteristic (ROC) curves. The prevalences of methylation for RASSF1A, APC, BVES, HOXA9, GSTP1, and TIMP3 were 52.04%, 36.73%, 29.59%, 20.41%, 17.35%, and 11.22%, respectively. APC methylation completely overlapped with RASSF1A methylation. The area under the curve (AUC) for RASSF1A methylation (0.718) was better than the corresponding AUC for AFP (0.609) in distinguishing HCC from CHB. When RASSF1A, BVES, HOXA9, and AFP were combined, the AUC was 0.852 (95% CI = 0.796–0.908, P=0.028), and the sensitivity and specificity were 83.7% and 78.9%, respectively. In conclusion, an assay that combines methylation of the RASSF1A, BVES, and HOXA9 gene promoters in serum and AFP could significantly improve HBV-related HCC diagnoses.

Aiming at the lack of standard design method and the shortcomings of main functions of the existing drive circuit, a “Element-level Design under Transmission Voltage Switch Theory” is set forth and a smallest circuit structure namely as drive-unit, which contains Superstratum-unit, Substratum-unit and LC-unit, is designed in this paper to achieve the necessary functions. The drive circuit of n×n ignition bridges has been got from the structure expansion formula along with the expected life estimation. The Ebers-Moll Model of transistor and the internal circuit of MOSFET were used to make a theoretical analysis and the conclusion of such analysis was verified by simulation subsequently. Actual measurement has been made and the results show that the circuit in simple structure has provided a strong loading ability and accurate output; some elements of the circuit have been heated when the circuit was working; and the response time of the circuit was of the microsecond-level.

A number of different semi-rigid pavement structure models are established to perform calculation and the results are led into the calculation software nSoft to analyze and acquire the fatigue property of different semi-rigid pavement structure models. The grey relational analysis of different semi-rigid pavement structure models fatigue properties are done based on the grey system theory to gain the key influence parameter on semi-rigid pavement structure fatigue performance. The research conclusion has certain instruction function to design pavement structure with excellent anti-fatigue performance.

Considering thermal power plant boiler’s nonlinear, large delay and time-varying, the paper proposes a compensatory fuzzy neural network control based on fuzzy control and neural network control. The compensatory fuzzy neural network is better than the PID controller and general fuzzy network controller in properties by using fuzzy inference and compensatory arithmetic. The paper makes a preliminary simulation using simulation tools of Matlab. And, the superiority of the compensatory fuzzy neural network control is proved by comparing the two kinds of simulation.

Recently, with the increasing strength of attracting business and investments in Liaoning coastal area or big cities’ suburbs (counties), a large number of industrial and mining enterprises have shifted to rural areas. The rural areas’ power load is growing rapidly, but at the same time, many power sites such as 66kv substation, 10kv transmission line, and low-voltage distribution area have occurred different levels of “low voltage”, which affect the daily life and production of native residents. From 2011, Liaoning Electric Power Co., Ltd. has applied the third generation of dynamic reactive power compensation technology—SVG to control the “low voltage” of rural network, which has fast speed and strong compensation. And as a consequence, there is good social and economic benefit.