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Although the role of platelet-rich plasma (PRP) in ultraviolet light B (UVB)-induced photoaging has been confirmed in many studies, the specific mechanism is still not clear. Therefore, we attempted to investigate the effect and mechanism of PRP on UVB-induced human keratinocyte (HaCaT cells) apoptosis.HaCaT cells were collected to construct UVB-induced photoaging models. Then, the cells were divided into Sham group, 5% PRP group, UVB group, and UVB + 5% PRP group. Next, MTT assay was used to detect the level of cell proliferation; flow cytometry to check the level of apoptosis; ELISA to determine the TNF-α, IL-18, IL-6, and IL-1β levels in the supernatant; and Western blot to test Bax, Bcl-2, cytochrome c (Cyt.c), GRP78, CHOP, and ATF4 protein expression levels.Briefly, 5% PRP intervention could relieve the inhibition of UVB on HaCaT cell proliferation, inhibit the promotion of UVB to cell apoptosis, up-regulate UVB-induced Bcl-2 protein expression, and decrease Bax and Cyt.c protein level. In addition, 5% PRP significantly down-regulated the inflammatory factor levels of TNF-α, IL-18, IL-6, and IL-1βin UVB-induced cells and reduced the inflammatory response. Moreover, 5% PRP also greatly reduced the protein expression levels of GRP78, CHOP, and ATF4 in UVB-induced cells and alleviated endoplasmic reticulum (ER) stress.PRP may protect HaCaT cells from UVB-induced apoptosis by alleviating inflammatory response and ER stress.

Use of Chinese herbal compounds have been widely explored in recent years to inhibit excessive proliferation and activation of astrocytes in brain injuries. Thus, it has been reported that Gastrodin, an anti-inflammatory agent, can effectively suppress microglia activation in rats with hypoxic-ischemia brain damage. This study was therefore aimed to determine if Gastrodin would exert a similar effect on reactive astrocytes and alleviate their inflammatory response through the renin-angiotensin system (RAS) and Sirtuin3 (SirT3). Expression of ATO, AT1 and SirT3 was detected in TNC-1 astrocytes, treated respectively with conditioned medium from BV-2 microglia with or without pretreatment of Gastrodin and lipopolysaccharide (LPS) by immunofluorescence and Western blotting. Along with this, expression of C3, S100A10, TNF-α and neurotrophic factors, including BDNF and IGF-1 expression was also evaluated. We showed here that TNC-1 astrocytes incubated in CM + LPS showed a marked increase in ATO, AT1, C3 and TNF-α when compared to cells in the BV-2 conditioned medium (CM), but that of S100A10 was reduced. Of note, TNC-1 astrocytes responded vigorously to conditioned medium derived from BV-2 microglia treated with Gastrodin + LPS when compared to the controls. This was evident by the decreased expression of the above-mentioned proteins, except for S100A10. As opposed to this, SirT3, IGF-1 and BDNF expression as determined by Western and immunofluorescence analysis was enhanced. The results suggest that Gastrodin inhibits the expression of RAS and proinflammatory mediators, but promotes the production of large amounts of neurotrophic factors in reactive astrocytes induced by LPS-stimulated activated microglia. Another novel finding of this study is the unequivocal demonstration that Gastrodin can promote the polarization of A2 phenotype that is regarded to be neuroprotective. This has added a new therapeutic perspective of this herbal compound that is gaining more attention in recent years.

IntroductionSyphilitic balanitis of Follmann (SBF) is a rare condition of primary syphilis which is characterized by any kind of balanitis with or without chancre on the penis combined with the presence of swollen inguinal lymph nodes confirmed by the finding of Treponema pallidum in the lesions or by the positive serological syphilitic testing. Timely identification of the SBF is very important in properly treating the disease stopping the spread of syphilis.Case presentationA 42year-old heterosexual male patient came to our clinic and complained of a painless, hard erythema nodule with a whitish scale in his coronal sulcus of the penis for about a week. The dermatologic examination revealed an infiltrative, hard erythematous lesion surrounding the coronal sulcus of the patient’s penis, with mild erosion and a small amount of exudation. There was a whitish pseudomembrane-like covering on the surface of the erythematous lesion in the coronal sulcus, which is mimicked as candidal balanitis. The result of the fungus microscopic examination was negative, while the laboratory findings showed positive results in serologic syphilitic testing. The patient was diagnosed with primary syphilis and intramuscularly treated with a dose of benzylpenicillin of 2.4 million units. The patient’s skin lesions disappeared completely 60 days after penicillin treatment.ConclusionTo our knowledge, this is the first SBF case reported in China. Syphilitic balanitis of Follmann may have variable clinical appearances. We emphasize that when balanitis with risky sexual activities or with sexually transmitted diseases, the diagnosis of SBF should be kept in mind.

To clarify the role of Nth gene in drug resistance of Mycobacterium tuberculosis (MTB), the clinical strain MTB were isolated and cultured from the sputum of pulmonary tuberculosis patients, and grouped according to the results of drug sensitivity tests. The expression levels of Nth mRNA and protein were detected by Polymerase chain reaction and Western blot, respectively. The bacteriophage-mediated homologous recombination gene knockout method and seamless cloning and ligation technology were used to knockout Nth gene and perform backfill overexpression experiments on MTB standard strain H37Rv. The resistance of the obtained strains was induced, and its growth was compared. In the results, the resistance rate of 193 clinical isolates to first-line drugs was 37.37%. The expression levels of Nth mRNA and protein in sensitive strains were higher than those in drug-resistant strains (P < 0.05). Strains without Nth were more likely to grow, while the growth of the Nthoverexpressing strain was inhibited. When two drugs are used in combination, the bacteria developed drug resistance later than those induced by single drug. In conclusion, the Nth gene has an inhibitory effect on the drug resistance of MTB.

Hand hygiene is essential to human health and day-to-day activities. Maintaining proper hand hygiene can help reduce pathogen growth and transmission of diseases. The automated sensor used for bathroom faucets and soap dispensers can be dated back to the 1950s. These types of fixtures are usually equipped with sensors using a near-infrared beam to detect hands. Its introduction has improved hand hygiene and facilitated good handwashing behaviour. At the same time, disadvantages that come along with advanced technologies should not be neglected, and these disadvantages may lead to inequity in the society.

BackgroundEpithelial–myoepithelial carcinoma (EMCa) is a rare low-grade malignant tumor that most commonly occurs in the salivary glands, with approximately 320 cases having been reported worldwide. Here, we report the third case of EMCa occurring in the nasopharynx. Rare cases in the breast, pituitary gland, lacrimal gland, nose, paranasal sinus, nasal cavity, trachea and bronchus, lung, and even the pleura mediastinalis have also been reported. Histopathology and immunohistochemistry are useful for confirming the diagnosis of EMCa, which is characterized by biphasic tubular structures composed of inner ductal and outer clear myoepithelial cells and stains for different markers in each layer. However, because of the rarity of EMCa, the clinicopathological characteristics and treatment of these patients remain unclear.Case presentationWe report a rare case of EMCa of the nasopharynx. A 51-year-old man presented with a 5-month history of pain while swallowing and aggravation accompanied by right ear tinnitus lasting for 1 month. Nasopharyngoscopy and magnetic resonance imaging (MRI) of the nasopharynx and neck revealed a 5.6 cm × 3.4 cm × 3.1 cm mass in the nasopharyngeal space, invasion of the right cavernous sinus, and lymph node enlargement in the right retropharyngeal space. On 17 April 2019, based on the histopathological and immunohistochemical features, a final diagnosis of EMCa of the right nasopharynx was made. The patient underwent concurrent chemoradiotherapy (CCRT), and his symptoms were relieved after treatment. On 10 January 2022, nasopharynx MRI and biopsy revealed local recurrence, but chest and abdominal computed tomography (CT) showed no obvious signs of metastasis. The local recurrence-free survival (LRFS) period was 33 months.ConclusionTo the best of our knowledge, this is the third reported case of EMCa in the nasopharynx and the only case of EMCa in the nasopharynx treated with CCRT, and a partial response was achieved. Therefore, to improve the quality of life and prognosis of patients with unresectable tumors, we believe that CCRT is a suitable option. Further clinical observations are required to elucidate the pathophysiology and prognosis of EMCa.

Anaerobic digestion (AD) is a potential bioprocess for waste biomass utilization and energy conservation. Various iron/carbon-based CMs (e.g., magnetite, biochar, granular activated carbon (GAC), graphite and zero valent iron (ZVI)) have been supplemented in anaerobic digestors to improve AD performance. Generally, the supplementation of CMs has shown to improve methane production, shorten lag phase and alleviate environmental stress because they could serve as electron conduits and promote direct interspecies electron transfer (DIET). However, the CMs dosage varied greatly in previous studies and CMs wash out remains a challenge for its application in full-scale plants. Future work is recommended to standardize the CMs dosage and recover/reuse the CMs. Moreover, additional evidence is required to verify the electrotrophs involved in DIET.

The anaerobic ammonium oxidation (anammox) process has the advantages of high efficiency and low energy consumption, so it has broad application prospects in biological denitrification of wastewater. However, the application of anammox technology to existing wastewater treatment is still challenging. The main problems are the insufficient supply of nitrite and the susceptibility of anammox bacteria to environmental factors. In this paper, from the perspective of the diversity of anammox bacteria, the habitats and characteristics of anammox bacteria of different genera were compared. At the same time, laboratory research and engineering applications of anammox technology in treating wastewater from different sources were reviewed, and the progress of and obstacles to the practical application of anammox technology were clarified. Finally, a focus for future research was proposed to intensively study the water quality barrier factors of anammox and its regulation strategies. Meanwhile, a combined process was developed and optimized on this basis.

Composting is an effective alternative for recycling faecal sludge into organic fertilisers. A microflora-earthworm (Eisenia fetida) synergistic cooperation system was constructed to enhance the composting efficiency of faecal sludge. The impact of earthworms and carbon-rich wastes (rice straw (RS) and sawdust (S)) on compost properties, greenhouse gas emissions, and key microbial species of composting were evaluated. The addition of RS or S promoted earthworm growth and reproduction. The earthworm-based system reduced the volatile solid of the final substrate by 13.19-16.24 % and faecal Escherichia coli concentrations by 1.89-3.66 log10 cfu/g dry mass compared with the earthworm-free system. The earthworm-based system increased electrical conductivity by 0.322-1.402 mS/cm and reduced C/N by 56.16-64.73 %. The NH

Simultaneously achieving efficient nitrogen (N) and phosphorus (P) removal without adding external carbon source is vital for carbon-neutral wastewater treatment. In this study, a novel cross-flow honeycomb bionic microbial carrier (CF) was developed to improve the efficiency of simultaneous nitrification, denitrification, and P removal (SNDPR) in an integrated fixed-film activated sludge (IFAS) system. A parallel laboratory-scale sequencing batch reactor with the commercialized microbial carriers (CM) (CM-IFAS) was performed as the comparative system for over 233 d The results demonstrated that CF-IFAS exhibited a more consistent N removal efficiency and better performance than CM-IFAS. In the CF-IFAS, the highest N and P removal efficiencies were 95.40% and 100%, respectively. Typical cycle analysis revealed that nitrate was primarily removed by the denitrifying glycogen-accumulating organisms in the CF-IFAS and by denitrifying phosphate-accumulating organisms in the CM-IFAS. The neutral community model showed that the microbial community assembly in both the reactors was driven by deterministic selection rather than stochastic factors. Compared to those in CM-IFAS, the microorganisms in CF-IFAS were more closely related to each other and had more keystone species: norank_f_norank_o_norank_c_OM190, SM1A02, Defluviicoccus, norank_f_ Saprospiraceae, and norank_f_Rhodocyclaceae. The absolute contents of the genes associated with N removal (bacterial amoA, archaeal amoA, NarG, NapA, NirS, and NirK) were higher in CF-IFAS than in CM-IFAS; the N cycle activity was also stronger in the CF-IFAS. Overall, the microecological environment differed between both systems. This study provides novel insights into the potential of bionic carriers to improve SNDPR performance by shaping microbial communities, thereby providing scientific guidance for practical engineering.

Inorganic semiconductors play an important role in fields such as information, sensor, and energy. But most bulk inorganic semiconductors are intrinsically brittle. With the development of smart electronic components like flexible electronic devices, it is urgent to explore plastically deformable inorganic semiconductors. For high-throughput screening of possible candidate materials, a reliable, universal, and convenient factor is needed. In this paper, a simple plastic deformability factor consisting of only elastic constants was proposed, which were examined by the experimentally verified plastic inorganic semiconductors Ag2S and InSe. Through data mining, 612 types of bulk layered materials were selected from more than 40,000 three-dimensional materials, two of which (PbI2 and CuInP2S6) were further experimentally verified, showing the reliability of this factor. This simple factor could be used to rapidly predict the plastic deformability of layered materials and large-scale screening of candidate inorganic materials for next-generation deformable and flexible electronic devices.

Hydrogen (H

External stimulus such as light irradiation is able to deteriorate intracellular redox homeostasis and induce photooxidative damage to non-photogenic bacteria. Exploiting effective strategies to help bacteria resisting infaust stress is meaningful for achieving a stable operation of biological treatment system. In this work, selenium-doped carbon quantum dots (Se-CQDs) were blended into anaerobic ammonia oxidation (anammox) bacteria and an inorganic nanoparticle-microbe hybrid was successfully fabricated to evaluate its nitrogen removal performance under solar-simulated irradiation. It was found that the specific anammox activity decreased by 29.7 ± 5.2% and reactive oxygen species (ROS) content increased by 134.8 ± 4.1% under 50,000 lux light. Sludge activity could be completely recovered under the optimum dosage of 0.42 mL·(g volatile suspended solid)

In this paper, an efficient and adjustable visual image encryption scheme is proposed by combining a 6D hyperchaotic system, compressive sensing, and Bezier curve embedding. First, the plain image is sparse by discrete wavelet transform (DWT). Then, the sparse image is encrypted and compressed through game-of-life (GOL) hybrid scrambling and compressive sensing into a cipher image. Next, Bezier curve embedding is utilized to embed the cipher image into the carrier image in wavelet domain. After these operations, the final visually meaningful steganographic image is generated. Additionally, the frequency-domain information of the plain image is used to generate the initial values of the 6D hyperchaotic system in scrambling process, which makes the proposed encryption scheme able to effectively resist the chosen-plaintext attacks (CPA) and the known-plaintext attacks (KPA). Moreover, our scheme exhibits excellent adjustable performance compared with existing related schemes. Ultimately, simulation results and comprehensive performance analyses demonstrate that the scheme proposed in this paper has high decryption quality, visual security, robustness, and operating efficiency.

The treatment of bladder cancers has improved with new immunotherapy such as PD-L1. However, not all bladder cancers respond to PD-L1 immunotherapy which is why new targets are being assessed. Melanoma-associated antigen gene A (MAGE-A) family proteins are expressed in a variety of tumors, with each MAGE-A protein having unique roles in cancer pathogenesis. One advantage of targeting MAGE-A family members is the lack of expression in normal tissues which makes them well-suited for targeted cancer immunotherapy. The challenge with screening the MAGE-A family is to find a specific antibody since the 12-member family has over 60% sequence in homology. In this study, we evaluate multiple MAGE-A3 and MAGE-A4 antibodies using CytoSections, a reproducible alternative to control tissue with an expression of target biomarkers. A series of antibodies specific to each MAGE-A3 and MAGE-A4 protein were discovered and used to screen twenty-five bladder cancer tissues. The results showed that MAGE-A3 was present in 9 of the 25 bladder cancers, while MAGE-A4 was present in 10 of the 25 bladder cancers evaluated; in the meantime, 7 bladder cancers were positive for both targets after double immunofluorescence staining with MAGE-A3 or A4 specific antibodies. The data presented here could be an important development in treatment protocols for pre-screening patients who are at risk. Our findings show that MAGE-A3 and MAGE-A4 can be both present in bladder cancer, suggesting that targeting both genes may be necessary for the success of immunotherapy treatment. Citation Format: Bailey Gilmore, Rachel Gonzalez, Aubrey Su, Dehe Kong, Eden Zewdu, Jina Yom, Tianli Qu, Zhaoying Guo, Xiaomin Hu, Qi Ren, Ranran Zhang, Eric Christenson, Yan Ma, Dezhong Yin, Patrick Yin, Wei Fu, Xuan Liu, Krishnan Allampallam. Colocalization of MAGE-A3 and MAGE-A4 in bladder cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 999.