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Glucagon receptor plays an important role in the regulation of glucose metabolism. Studies have revealed that glucagon receptor antagonism is a potential effective treatment for diabetes. However, the functions of GCGR have not been fully illustrated. Although two Gcgr truncation knockout mice models have been widely used for GCGR function studies, truncated gene may remain neomorphic and/or dominant-negative function. In this study, we took the advantages of Crispr-Cas9 technique and generated a novel allele of GCGR in the mouse that yields complete loss of GCGR protein. Our studies reveal that complete deletion of Gcgr results in hyperglucagonemia, α-cell hyperplasia, improvement of glucose tolerance. These results are similar to the Gcgr-truncated mutation in mice. Hence, we provide a novel strain of GCGR knockout mice for the GCGR function studies.

An electronegativity-dominant high-entropy atomic environment regulation strategy was developed to manipulate the electrocatalytic properties by tailoring the competitive adsorption sites in HEA NPs.

Inductively coupled plasma-mass spectrometry (ICP-MS) with elemental labeling is a promising strategy for multiplex microRNA (miRNA) analysis. However, it is still challenging for specific analysis of multiple miRNAs with high homology, and the development of multiplex assays is always limited by the complexity of the sequence design. Herein, a simple and direct ICP-MS-based assay was developed for the simultaneous detection of three miRNAs by combining the lanthanide labeling strategy with entropy-driven catalytic (EDC) amplification. Owing to the specificity of EDC for nucleic acid recognition, it is able to differentiate miRNAs with single-base mutation in each EDC circuit. A universal biotin-labeled DNA strand was designed to hybridize with the DNA substrates for three EDC circuits, targeting miRNA-21, miRNA-155, and miRNA-10b, respectively. All the substrates were loaded on the surface of streptavidin magnetic beads. In the presence of target miRNA, the EDC reaction was initiated, and EDC substrates were dissociated, continuously releasing reporter strands that were labeled with lanthanides (Tb/Ho/Lu). After magnetic separation, the supernatant containing the released reporter strands was introduced into an ICP-MS system for simultaneous detection of

In order to meet the requirements of online fault detection for dry reactor, an online fault detection technology based on improved Kalman filter is proposed. The main content of the technology is based on the dry reactor detection technology, through the study of improved Kalman filter, the use of fault diagnosis and other methods, and finally through the experiments and analysis to build improved Kalman filter dry reactor online fault detection research means. The experimental results show that the maximum relative error of the improved Kalman filter is 6.039%, and the average relative error is 2.388%. The improved algorithm is very effective and greatly improves the prediction accuracy. The research based on improved Kalman filter can meet the demand of online fault detection of reactor.

Aim: To evaluate the efficacy and safety of corticosteroids for treating hospitalized COVID-19 patients. Materials & methods: Efficacy outcomes included time to negative SARS-CoV-2 tests, length of stay, duration and incidence of intensive unit care stay, incidence of mortality and duration and incidence of mechanical ventilation. Safety outcomes included the incidence of adverse events and severe adverse events, incidence of hyperglycemia and incidence of nosocomial infections. Results: Ninety-five randomized controlled trials (RCTs) and observational studies (n = 42,205) were included. Corticosteroids were associated with increased length of stay (based on RCT only), increased time to negative tests, decreased length of mechanical ventilation and increased odds of hyperglycemia. Conclusion: Corticosteroids should be considered in patients requiring mechanical ventilation, and glycemic monitoring may be needed when administering corticosteroids.

Ecosystem services are the beneficial goods and services that ecosystems provide to humans. Urbanization is an important feature of human social development. While promoting economic and social development, it also brings about land degradation, resource depletion, environmental pollution and other problems, intensifying the transformation of natural ecosystems into semi-natural and artificial ecosystems, ultimately leading to the loss of ecosystem service functions and declining value. The study of the impact of urbanization on the value of ecosystem services is of critical importance for the conservation of ecosystems and sustainable development. This study examined the spatio-temporal patterns of urbanization’s impacts on ecosystem service value in the Yellow River Basin from the perspective of spatio-temporal heterogeneity. Findings: (1) Both the ecosystem service value (ESV) and urbanization level (UL) in the Yellow River Basin were on the rise on the whole, but they were significantly spatially negatively correlated and mainly characterized by the high–low spatial clustering of “low ESV–high UL” and “high ESV–low UL”. This negative correlation was gradually weakened with the transformation of the urbanization development mode and ecological restoration projects in the Yellow River Basin. (2) The impacts of the five urbanization subsystems on the value of ecosystem services were diverse. Landscape urbanization had a negative impact on the value of ecosystem services in all regions; economic urbanization and innovation urbanization changed from having a negative to a positive impact; and demographic urbanization and social urbanization had both a positive and a negative impact. (3) To promote the coordinated development of ecological environmental protection and urbanization in the YRB, this paper proposes to change the urbanization development model, implement ecological restoration by zoning, and formulate classified development plans. This study compensates for the shortcomings of current studies that ignore the different impacts of urbanization subsystems on ecosystem service value and lack sufficient consideration of the spatio-temporal heterogeneity characteristics of urbanization and ESVs, enriches the theoretical understanding of the interrelationships between natural and human systems in basin areas, and provides a scientific basis for the rational formulation of urban planning and ecological protection policies in the region, which is of great theoretical and practical significance.

(1) Background: To examine the relationship among just-world beliefs, self-control, and cyberaggression among college students. (2) Methods: A total of 1133 college students were surveyed using the just-world belief scale, self-control scale, and cyberaggression scale. (3) Results: The results showed that college students with low levels of belief in justice frequently showed cyberaggression; belief in a just world directly and negatively predicted cyberaggression, and indirectly predicted student cyberaggression through self-control; gender moderated the indirect effect of self-control on cyberaggression and the direct effect of belief in a just world on cyberaggression. (4) Conclusions: Belief in a just world significantly and negatively predicts cyberaggression; self-control has an indirect significant effect on cyberaggression; the direct effect of belief in a just world on cyberaggression and the mediating effect of self-control on this association are moderated by gender.