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Alien species represent a significant component of European entomofauna. Intensive international trade of plants, particularly woody ornamentals, has increased introduction of alien species into Europe in the last two decades, where alien terrestrial invertebrates prevail as one of the most numerous groups of introduced organisms. As of 2010, fourteen species of alien psyllids (Hemiptera: Psylloidea) have been recorded in Europe. Five species have been reported from Croatia, including Acizzia jamatonica (Kuwayama, 1908), as the only species from genus Acizzia Heslop-Harrison, 1961 present in Croatia so far. This paper provides genus status in Croatia, with first records of Australian species Acizzia uncatoides (Ferris & Klyver, 1932) and Acizzia acaciaebaileyanae (Froggatt, 1901).

Recreational water use is an important source of human enteric illness. Enhanced (episodic) surveillance of natural recreational waters as a supplement to beach monitoring can enrich our understanding of human health risks. From 2011 to 2013, water sampling was undertaken at recreational sites on a watershed in eastern Canada. This study compared the prevalence and associations of human enteric pathogens and fecal indicator organisms. Beach water samples had lower pathogen presence than those along the main river, due to different pollution sources and the hydrological disposition. Pathogen profiles identified from the beach sites suggested a more narrow range of sources, including birds, indicating that wild bird management could help reduce public health risks at these sites. The presence and concentration of indicator organisms did not differ significantly between beaches and the river. However, higher concentrations of generic Escherichia coli were observed when Salmonella and Cryptosporidium were present at beach sites, when Salmonella was present at the river recreational site, and when verotoxigenic E. coli were present among all sites sampled. In this watershed, generic E. coli concentrations were good indicators of potential contamination, pathogen load, and elevated human health risk, supporting their use for routine monitoring where enhanced pathogen testing is not possible. HIGHLIGHTS Enhanced surveillance of recreational waters can supplement water quality monitoring and inform our understanding of what contributes to the risk of human illness.; Water collected at reservoir beaches was less contaminated than sites along the main river.; Subtyping of pathogens at the beach sites suggested a narrow range of sources.; The nature of the sampling site influenced the prevalence and types of pathogens.;

Abstract Patch size is one of the most important factors affecting the distribution and abundance of species, and recent research has shown that patch size is an important niche dimension affecting community structure in aquatic insects. Building on this result, we examined the impact of patch size in conjunction with presence of larval anurans on colonization by aquatic insects. Hyla chrysoscelis (Cope's gray treefrog) larvae are abundant and early colonists in fishless lentic habitats, and these larvae can fill multiple ecological roles. By establishing larvae in mesocosms prior to colonization, we were able to assess whether H. chrysoscelis larvae have priority effects on aquatic insect assemblages. We conducted a series of three experiments in naturally colonized experimental landscapes to test whether (1) H. chrysoscelis larval density affects insect colonization, (2) variation in patch size affects insect colonization, and (3) the presence and larval density of H. chrysoscelis shift colonization of insects between patches of different size. Larval density independently had almost no effect on colonization, while patch size had species‐specific effects consistent with prior work. When larvae and patch size were tested in conjunction, patch size had numerous, often strong, species‐specific effects on colonization; larval density had effects largely limited to the assemblages of colonizing beetles and water bugs, with few effects on individual species. Higher larval densities in large mesocosms shifted some insect colonization to smaller patches, resulting in higher beta diversity among small patches in proximity to high density large mesocosms. This indicates establishing H. chrysoscelis larvae prior to insect colonization can likely create priority effects that slightly shape insect communities. Our results support the importance of patch size in studying species abundances and distributions and also indicate that colonization order plays an important role in determining the communities found within habitat patches.

Obilježjima i značajkama slikovnica bave se brojni stručni i znanstveni radovi. Najvećim dijelom oni su upućeni pedagoškom kadru koji u institucionalnom kontekstu, vođen standardima poticajnog čitanja i kvalitetne slikovnice, posreduje djeci njima namijenjenu knjigu. Ipak, poput brojnih stavova, čitalačke navike dijete dominantno formira u domu svoje obitelji, pa je bitan i istraživački uvid u roditeljsku procjenu važnosti prve djetetove knjige. U radu se predstavljaju rezultati anketnog ispitivanja kojim se utvrđuje roditeljska procjena važnosti slikovnice. Inicijalno se pretpostavlja da su dob i stupanj obrazovanja povezani s roditeljskim pristupom knjizi. Predviđa se da oni više razine obrazovanja i starije životne dobi pozitivnije percipiraju slikovnicu. Provedenim istraživanjem te su pretpostavke odbačene.

U radu se tematizira odgojna pozicija djeteta u obitelji i društvu. Afirmacija suvremenog djeteta kao socijalnog aktera – osobe autonomnih prava, u velikoj je mjeri doprinijela uvažavajućem pristupu odraslih spram njega. Istovremeno, određivanje djeteta kao subjekta koji u velikoj mjeri određuje svoj život i razvoj, odgojnu ulogu odraslog čini prilično nedorečenom. Suvremeni odgojitelj učestalo je poistovjećen s poticateljem razvoja. Društveni život okarakteriziran konkurentskim suživotom jedinki u natjecateljskom okruženju orijentira odrasle na ustrajanje u dominantnom doprinošenju spoznajama djeteta. Budu- ći da je takav pristup odgojno nedostatan, ovim se radom tematizira pedagoški odnos promotren u Frommovoj perspektivi. Upućuje se na orijentiranost na brigu, odgovornost, poštovanje i znanje kao temeljne preduvjete odgoja samosigurnog, pouzdanog, socijalno zainteresiranog i empatičnog djeteta.

Acid deposition from fossil fuel combustion caused calcium depletion in eastern North America during the 20th century, and calcium is a critical resource for birds during both the egg laying and nesting periods. The effects of acid deposition on animals have largely acted through trophic relationships, with reduced calcium availability in prey possibly being a limiting factor for forest songbirds. Following 21 years of experimental whole-watershed acidification at the Bear Brook Watershed in Maine, we investigated Ovenbird (Seiurus aurocapilla) territory size and potential prey (leaf litter arthropod) abundance, assemblage, and calcium concentration during one breeding season. We delineated and estimated the area of 8 Ovenbird territories in an acidified and a control watershed, but found that territory size did not differ between watersheds. However, while calcium concentrations of potential prey also did not differ between watersheds, territory size decreased as calcium concentrations of potential prey increased, regardless of watershed. Our results suggest that calcium limitation can be a determinant of territory size in some landscapes, and this provides further support to importance of the role of calcium in the lives of passerines. Received 17 June 2020. Accepted 1 October 2021. Key words: acid deposition, breeding ecology, calcium, nutrient limitation, trophic interactions. La deposicion de acido producto de la quema de combustibles fosiles causo perdidas dc calcio en el este de Norteamerica durante cl siglo veintc y el calcio es un recurso critico para las aves durante los periodos de puesta de huevos y anidacion. En su mayoria, los efectos de la deposicion de acido en animales han operado por medio de relaciones troficas, donde una reduccion en la disponibilidad de calcio en sus presas es posiblcmente un factor limitante para aves canoras de bosque. Siguiendo 21 afios de acidification experimental de toda la cuenca en la Bear Brook Watershed en Maine, investigamos el tamano del territorio y la abundancia, ensamble y concentracion de calcio de las presas potenciales (artropodos de la hojarasca) del chipe Seiurus aurocapilla durante una temporada reproductiva. Delineamos y estimamos el area de 8 territorios dc este chipe cn una cuenca acidificada y una cuenca control, pcro encontramos que el tamano del territorio no difiere entre estas. Sin embargo, aunque las concentraciones de calcio de sus presas potenciales tampoco difieren entre cuencas, el tamano de territorio decrccio mientras que la concentration de calcio de presas potenciales se incremento independientemente dc la cuenca. Nuestros resultados sugieren que la [imitation de calcio puede ser una determinantc de tamano de territorio en algunos paisajes y esto provee mayor soporte a la importancia del calcio en la vida de las paserinas. Palabras clave: calcio, deposition de acido. ecologia de la reproduccion. interacciones troficas. limitation de nutrientes., Effects of acid deposition from fossil fuel combustion on ecosystems came to the forefront of society's ecological consciousness in the latter half of the twentieth century (Lynch et al. 2000). [...]

Abstract Intra-abdominal infections (IAIs) are common surgical emergencies and have been reported as major contributors to non-trauma deaths in hospitals worldwide. The cornerstones of effective treatment of IAIs include early recognition, adequate source control, appropriate antimicrobial therapy, and prompt physiologic stabilization using a critical care environment, combined with an optimal surgical approach. Together, the World Society of Emergency Surgery (WSES), the Global Alliance for Infections in Surgery (GAIS), the Surgical Infection Society-Europe (SIS-E), the World Surgical Infection Society (WSIS), and the American Association for the Surgery of Trauma (AAST) have jointly completed an international multi-society document in order to facilitate clinical management of patients with IAIs worldwide building evidence-based clinical pathways for the most common IAIs. An extensive non-systematic review was conducted using the PubMed and MEDLINE databases, limited to the English language. The resulting information was shared by an international task force from 46 countries with different clinical backgrounds. The aim of the document is to promote global standards of care in IAIs providing guidance to clinicians by describing reasonable approaches to the management of IAIs.

Tennis, as one of the most popular individual sports in the world, holds an important role in the betting world. There are two main categories of bets: pre-match betting, which is conducted before the match starts, and live betting, which allows placing bets during the sporting event. Betting systems rely on setting sports odds, something historically done by domain experts. Setting odds for live betting represents a challenge due to the need to follow events in real-time and react accordingly. In tennis, hierarchical models often stand out as a popular choice when trying to predict the outcome of the match. These models commonly leverage a recursive approach that aims to predict the winner or the final score starting at any point in the match. However, recursive expressions inherently contain computational complexity which hinders the efficiency of methods relying on them. This paper proposes a more resource-effective alternative in the form of a combinatorial approach based on a binomial distribution. The resulting accuracy of the combinatorial approach is identical to that of the recursive approach while being vastly more efficient when considering the execution time, making it a superior choice for live betting in this domain.

Clostridioides difficile colonization and development of infection commonly occur in inflammatory bowel disease (IBD) patients and can trigger flare-ups. Both conditions are inherently linked to disrupted gut microbiota. This study included 149 hospitalized gastrointestinal patients, which were divided into IBD (n = 48) and non-IBD patients (n = 101). Patients were tested for C. difficile colonization (qPCR and selective plating), and gut bacterial communities were analyzed with 16S amplicon sequencing. Blood test results were retrospectively collected from the medical records. IBD and non-IBD patients had comparable C. difficile colonization rates (31.7 and 33.3%, respectively). Compared to non-IBD C. difficile-non-colonized patients, IBD and C. difficile-colonized patients shared multiple common bacterial community characteristics including decreased diversity and reduced abundance of strict anaerobic bacteria. Furthermore, certain microbiota alterations were enhanced when IBD was accompanied by C. difficile colonization, indicating a synergistic effect between both medical complications. Conversely, certain microbial patterns were specific to C. difficile colonization, e.g., co-occurrence with Enterococcus, which was most common in IBD patients (81.3%).

ABSTRACT Fitness benefits from division of labor are well documented in microbial consortia, but the dependency of the benefits on environmental context is poorly understood. Two synthetic Escherichia coli consortia were built to test the relationships between exchanged organic acid, local environment, and opportunity costs of different metabolic strategies. Opportunity costs quantify benefits not realized due to selecting one phenotype over another. The consortia catabolized glucose and exchanged either acetic or lactic acid to create producer-consumer food webs. The organic acids had different inhibitory properties and different opportunity costs associated with their positions in central metabolism. The exchanged metabolites modulated different consortial dynamics. The acetic acid-exchanging (AAE) consortium had a “push” interaction motif where acetic acid was secreted faster by the producer than the consumer imported it, while the lactic acid-exchanging (LAE) consortium had a “pull” interaction motif where the consumer imported lactic acid at a comparable rate to its production. The LAE consortium outperformed wild-type (WT) batch cultures under the environmental context of weakly buffered conditions, achieving a 55% increase in biomass titer, a 51% increase in biomass per proton yield, an 86% increase in substrate conversion, and the complete elimination of by-product accumulation all relative to the WT. However, the LAE consortium had the trade-off of a 42% lower specific growth rate. The AAE consortium did not outperform the WT in any considered performance metric. Performance advantages of the LAE consortium were sensitive to environment; increasing the medium buffering capacity negated the performance advantages compared to WT. IMPORTANCE Most naturally occurring microorganisms persist in consortia where metabolic interactions are common and often essential to ecosystem function. This study uses synthetic ecology to test how different cellular interaction motifs influence performance properties of consortia. Environmental context ultimately controlled the division of labor performance as shifts from weakly buffered to highly buffered conditions negated the benefits of the strategy. Understanding the limits of division of labor advances our understanding of natural community functioning, which is central to nutrient cycling and provides design rules for assembling consortia used in applied bioprocessing.

Mitragynine (MG) is an indole alkaloid from kratom plant that binds opioid receptors and as such presents a scaffold for the development of atypical opioid receptor modulators. Here, the authors report a synthetic method for selective functionalization of the C11 position of MG, and show that this position is essential for fine-tuning opioid receptor signaling efficacy.

Abstract Background Despite the current therapeutic options for the treatment of inflammatory bowel disease, surgery is still frequently required in the emergency setting, although the number of cases performed seems to have decreased in recent years. The World Society of Emergency Surgery decided to debate in a consensus conference of experts, the main pertinent issues around the management of inflammatory bowel disease in the emergent situation, with the need to provide focused guidelines for acute care and emergency surgeons. Method A group of experienced surgeons and gastroenterologists were nominated to develop the topics assigned and answer the questions addressed by the Steering Committee of the project. Each expert followed a precise analysis and grading of the studies selected for review. Statements and recommendations were discussed and voted at the Consensus Conference of the 6th World Society of Emergency Surgery held in Nijmegen (The Netherlands) in June 2019. Conclusions Complicated inflammatory bowel disease requires a multidisciplinary approach because of the complexity of this patient group and disease spectrum in the emergency setting, with the aim of obtaining safe surgery with good functional outcomes and a decreasing stoma rate where appropriate.

Abstract Sport4Health Network (SPORT4H) is a multidisciplinary project co-funded by the European Union Erasmus+ programme aimed to encourage participation in physical activity in working population. SPORT4H includes educational and instructional activities that provide top-notch knowledge on various physical activities that may have an additional benefit to improve healthy lifestyle behaviours across workforce. The aims of Sport4Health 2021 e-symposium organized from 22nd to 23th March 2021 were to: (1) summarize data collected during this project through evaluation of health and fitness profiles for over 40,000 employees from all Sport4HealthNet countries (Belgium, Bulgaria, Croatia, Netherlands, Serbia and Slovenia); (2) discuss the applicability of user-friendly guidelines for physical activity at workplace and e-learning module that includes multicomponent interventions with innovative activities; (3) share experiences from different partners about the effects of educational interventions in specific working environment; and, (4) overview challenges identified during the implementation of interventions at work settings. The Sport4Health 2021 e-symposium facilitated networking between partner institutions, provided practical information for extensive public education that advances physical activity at workplace, and capacitated interaction and recruitment of end-users through e-learning modules and guidelines.

Surgical site infections (SSIs) are the most common adverse event occurring in surgical patients. Optimal prevention of SSIs requires the bundled integration of a variety of measures before, during, and after surgery. Surgical antibiotic prophylaxis (SAP) is an effective measure for preventing SSIs. It aims to counteract the inevitable introduction of bacteria that colonize skin or mucosa into the surgical site during the intervention. This document aims to guide surgeons in appropriate administration of SAP by addressing six key questions. The expert panel identifies a list of principles in response to these questions that every surgeon around the world should always respect in administering SAP.

Background: Urogenital schistosomiasis caused by Schistosoma haematobium is highly endemic in the municipality of Cubal in Angola. Currently, diagnosis is based on the observation of S. haematobium eggs in urine samples by microscopy but this method has low sensitivity. Few studies have been performed using molecular techniques in high-prevalence areas for the detection of S. haematobium. The objective of this study is to evaluate the usefulness of real-time PCR as a diagnostic technique for urogenital schistosomiasis among preschool-age children and its correlation with morbidity data. Methods: A cross-sectional study was conducted in Cubal, Angola, involving 97 urine samples from preschool-age children analyzed by the dipstick test, microscopic examination of filtered urine, and real-time PCR. The diagnosis of urogenital schistosomiasis was based on microscopy and/or real-time PCR results. Clinical and ultrasonography evaluation was performed to rule out complications of schistosomiasis. Results: We detected a total of 64.95% of samples positive by real-time PCR and 37.11% by microscopy. The sensitivity of parasitological diagnosis of urogenital schistosomiasis by real-time PCR and microscopy was 95.45% and 54.55%, respectively, and the sensitivity of real-time PCR compared with microscopy was 91.67%. A positive real-time PCR result was significantly related to older age (mean = 3.22 years), detection of eggs by microscopy, and abnormal urine dipstick results (18.56% with proteinuria, 31.96% with leukocyturia, and 31.96% with microhematuria) (p-value<0.05). Ultrasound analysis showed that 23.94% of children had urinary tract abnormalities, and it was significantly related to the real-time PCR diagnosis (p-value<0.05). Conclusions: Real-time PCR is a more sensitive technique than microscopy for urinary schistosomiasis diagnosis in preschool-age children in Cubal. This increase in sensitivity would allow earlier diagnosis and treatment, thus reducing the morbidity associated with schistosomiasis in its early stages. Author summary: Schistosomiasis is a neglected tropical disease that causes approximately 200,000 deaths worldwide each year. Urogenital schistosomiasis is caused by Schistosoma haematobium, which ultimately leads to lesions in the urinary tract due to egg deposition by the parasite. The World Health Organization recommends regular mass administration of praziquantel to prevent chronicity of the disease in high-endemicity areas. Preschool children are one of the most vulnerable groups, however, only children over two years of age are included in these strategies. The diagnostic techniques commonly used in endemic areas for egg detection by microscopy and urine hematuria have major limitations due to their low sensitivity. These limitations lead to misdiagnosis and undetected infections may eventually lead to the chronic form of the disease. Real-time PCR and microscopy were used to diagnose urogenital schistosomiasis in preschool children in Cubal, a high-transmission municipality in Angola. Urinary tract lesions were assessed by ultrasound. Of the samples tested, 64.95% were positive by real-time PCR, compared with 37.11% by microscopy, indicating sensitivities of 95.45% and 54.55%, respectively. Ultrasound analysis showed urinary tract alterations in 23.94% of the children. The results suggest that transmission of urogenital schistosomiasis in preschool children in Cubal is higher than previously estimated. [ABSTRACT FROM AUTHOR]

Metabolic and bariatric surgery (MBS) effectively treats obesity and related comorbidities, though individual responses vary. This systematic review examines how genetic variants influence MBS outcomes in morbidly obese patients. A comprehensive search in PubMed, Embase, Medline, and the Cochrane Library identified 1572 studies, with 52 meeting the inclusion criteria. Two reviewers independently filtered and selected studies, including relevant cross-references. Research focused on polymorphisms in genes such as UCP2, UCP3, 5-HT2C, MC4R, FKBP5, FTO, CAT haplotypes, LYPAL-1, PTEN, FABP-2, CNR1, LEP656, LEP223, GLP-1R, APOA-1, APOE, ADIPOQ, IL-6, PGC1a, TM6SF2, MBOAT7, PNPLA3, TCF7L2, ESR1, GHSR, GHRL, CD40L, DIO2, ACSL5, CG, TAS2R38, CD36, OBPIIa, NPY, BDNF, CLOCK, and CAMKK2. Most studies explored associations with post-surgery weight loss, while some examined metabolic, cardiovascular, taste, and eating behavior effects as well. Understanding the role of genetic factors in weight loss and metabolic outcomes post-MBS can help tailor personalized treatment plans for improved efficacy and long-term success. Further research with larger sample sizes and extended follow-up is needed to clarify the effects of many genetic variants on MBS outcomes in morbidly obese patients. [ABSTRACT FROM AUTHOR]

In uneven-aged forests, trees of different diameters, heights, and ages are located in a small area, which is due to the felling of individual trees or groups of trees, as well as small-scale natural disturbances. In this article, we present an objective method for classifying forest stands into even- and uneven-aged stands based on freely available low-resolution (with an average recording density of 5 points/m2) national lidar data. The canopy closure, dominant height, and canopy height diversity from the canopy height model and the voxels derived from lidar data were used to classify the forest stands. Both approaches for determining forest structural diversity (canopy height diversity—CHDCHM and CHDV) yielded similar results, namely two clusters of even- and uneven-aged stands, although the differences in vertical diversity between even- and uneven-aged stands were greater when using CHM. The first analysis, using CHM for the CHD assessment, estimated the uneven-aged forest area as 49.3%, whereas the second analysis using voxels estimated it as 34.3%. We concluded that in areas with low laser scanner density, CHM analysis is a more appropriate method for assessing forest stand height heterogeneity. The advantage of detecting uneven-aged structures with voxels is that we were able to detect shade-tolerant species of varying age classes beneath a dense canopy of mature, dominant trees. The CHDCHM values were estimated to be 1.83 and 1.86 for uneven-aged forests, whereas they were 1.57 and 1.58 for mature even-aged forests. The CHDV values were estimated as 1.50 and 1.62 for uneven-aged forests, while they were 1.33 and 1.48 for mature even-aged forests. The classification of stands based on lidar data was validated with data from measurements on permanent sample plots. Statistically significantly lower average values of the homogeneity index and higher values of the Shannon–Wiener index from field measurements confirm the success of the classification of stands based on lidar data as uneven-aged forests. [ABSTRACT FROM AUTHOR]

AbstractWe analyzed the efficacy and safety of aminoglycosides in a retrospective study of 415 patients with acute appendicitis and 277 patients with acute cholecystitis. The following variables increased the incidence of postoperative complications, defined as surgical site infection, recurrent intraabdominal infection, non-infectious post-operative complication, or death: age (p = 0.016 and 0.011), kidney disease (p = 0.019 and <0.001), and ASA Score (p < 0.001). The type of antibiotic therapy did not have a statistically significant effect on the incidence of postoperative complications in patients with acute appendicitis and cholecystitis (p = 0.561 and 0.547, respectively). A linear regression model showed a higher complication rate in patients with kidney disease (p = 0.014) and neoplasms (p = 0.013); the type of antibiotic therapy did not have a significant effect on the outcome (p = 0.765). There was no statistically significant difference in the post-treatment levels of creatinine in patients treated with aminoglycosides (gentamicin 3 mg/kg once daily) and in those who received other antibiotics (p = 0.75). [ABSTRACT FROM AUTHOR]

In this study, we pioneered the synthesis of nanoflower-shaped TiO2-supported Au photocatalysts and investigated their properties. Au nanoflowers (Au NFs) were prepared by a Na-citrate and hydroquinone-based preparation method, followed by wet impregnation of the derived Au NFs on the surface of TiO2 nanorods (TNR). A uniform and homogeneous distribution of Au NFs was observed in the TNR + NF(0.7) sample (lower Na-citrate concentration), while their distribution was heterogeneous in the TNR + NF(1.4) sample (higher Na-citrate concentration). The UV-Vis DR spectra revealed the size- and shape-dependent optical properties of the Au NFs, with the LSPR effect observed in the visible region. The solid-state EPR spectra showed the presence of Ti3+, oxygen vacancies and electron interactions with organic compounds on the catalyst surface. In the case of the TNR + NF(0.7) sample, high photocatalytic activity was observed in the H2-assisted reduction of NO2 to N2 at room temperature under visible-light illumination. In contrast, the TNR + NF(1.4) catalyst as well as the heat-treated samples showed no ability to reduce NO2 under visible light, indicating the presence of deformed Au NFs limiting the LSPR effect. These results emphasized the importance of the choice of synthesis method, as this could strongly influence the photocatalytic activity of the Au NFs. [ABSTRACT FROM AUTHOR]

Copyright of Gozdarski Vestnik is the property of Federation of Forestry Associations of Slovenia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study aimed to explore the impact of varying amounts of added Au (0.5 to 2 wt.%) and the structural characteristics of anatase TiO2 supports (nanoparticles (TP, SBET = 88 m2/g) and nanorods (TR, SBET = 105 m2/g)) on the catalytic efficiency of TiO2+Au catalysts in eliminating the herbicide glyphosate from aqueous solutions via the catalytic wet air oxidation (CWAO) process. The investigation was conducted using a continuous-flow trickle-bed reactor. Regardless of the TiO2 support and the amount of Au added, the addition of Au has a positive effect on the glyphosate degradation rate. Regarding the amount of Au added, the highest catalytic activity was observed with the TP + 1% Au catalyst, which had a higher Schottky barrier (SB) than the TP + 2% Au catalyst, which helped the charge carriers in the TiO2 conduction band to increase their reduction potential by preventing them from returning to the Au. The role of glyphosate degradation product adsorption on the catalyst surface is crucial for sustaining the long-term catalytic activity of the investigated TiO2+Au materials. This was particularly evident in the case of the TR + 1% Au catalyst, which had the highest glyphosate degradation rate at the beginning of the CWAO experiment, but its catalytic activity then decreased over time due to the adsorption of glyphosate degradation products, which was favoured by the presence of strong acidic sites. In addition, the TR + 1% Au solid had the smallest average Au particle size of all analyzed materials, which were more easily deactivated by the adsorption of glyphosate degradation products. The analysis of the degradation products of glyphosate shows that the oxidation of glyphosate in the liquid phase involves the rupture of C–P and C–N bonds, as amino-methyl-phosphonic acid (AMPA), glyoxylic acid and sarcosine were detected. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Slovenica is the property of Biotechnical Faculty of the University of Ljubljana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Predators are a particularly critical component of habitat quality, as they affect survival, morphology, behavior, population size, and community structure through both consumptive and non‐consumptive effects. Non‐consumptive effects can often exceed consumptive effects, but their relative importance is undetermined in many systems. Our objective was to determine the consumptive and non‐consumptive effects of a predaceous aquatic insect, Notonecta irrorata, on colonizing aquatic beetles. We tested how N. irrorata affected survival and habitat selection of colonizing aquatic beetles, how beetle traits contributed to their vulnerability to predation by N. irrorata, and how combined consumptive and non‐consumptive effects affected populations and community structure. Predation vulnerabilities ranged from 0% to 95% mortality, with size, swimming, and exoskeleton traits generating species‐specific vulnerabilities. Habitat selection ranged from predator avoidance to preferentially colonizing predator patches. Attraction of Dytiscidae to N. irrorata may be a natural ecological trap given similar cues produced by these taxa. Hence, species‐specific habitat selection by prey can be either predator‐avoidance responses that reduce consumptive effects, or responses that magnify predator effects. Notonecta irrorata had both strong consumptive and non‐consumptive effects on populations and communities, while combined effects predicted even more distinct communities and populations across patches with or without predators. Our results illustrate that an aquatic invertebrate predator can have functionally unique consumptive effects on prey, attracting and repelling prey, while prey have functionally unique responses to predators. Determining species‐specific consumptive and non‐consumptive effects is important to understand patterns of species diversity across landscapes.

Summary: Recombinant protein production can cause severe stress on cellular metabolism, resulting in limited titer and product quality. To investigate cellular and metabolic characteristics associated with these limitations, we compare HEK293 clones producing either erythropoietin (EPO) (secretory) or GFP (non-secretory) protein at different rates. Transcriptomic and functional analyses indicate significantly higher metabolism and oxidative phosphorylation in EPO producers compared with parental and GFP cells. In addition, ribosomal genes exhibit specific expression patterns depending on the recombinant protein and the production rate. In a clone displaying a dramatically increased EPO secretion, we detect higher gene expression related to negative regulation of endoplasmic reticulum (ER) stress, including upregulation of ATF6B, which aids EPO production in a subset of clones by overexpression or small interfering RNA (siRNA) knockdown. Our results offer potential target pathways and genes for further development of the secretory power in mammalian cell factories.

Abstract Although consumptive effects of predators have long been central to ecology, predation‐risk effects have emerged as major components of predator–prey interactions. Both consumptive and predation‐risk effects should vary with predator functional traits (e.g., hunting mode, gape size), where consumption rates and induction of morphological and behavioral defenses correlate with prey‐specific predator threat. Ambush predators, in contrast with active predators, may face selection pressure to be cryptic to avoid detection by prey. Thus, ambush predators may change prey density through consumptive effects but have reduced or absent predation‐risk effects. We performed two mesocosm experiments with free‐roaming and caged predators to explore post‐colonization interactions of the chemically camouflaged, large‐gaped, ambush predator, pirate perch (Aphredoderus sayanus), active, large‐gaped green sunfish (Lepomis cyanellus), and active, small‐gaped golden topminnow (Fundulus chrysotus), with larval gray treefrogs (Hyla chrysoscelis) and mole salamanders (Ambystoma talpoideum). We examined the consumptive and predation‐risk effects of each predator on amphibian mortality, growth rates, tail morphology, and polyphenisms. Large‐gaped pirate perch and green sunfish had strong, equivalent consumptive effects, but only the free‐roaming active predator, green sunfish, suppressed the growth of survivors through risk‐induced trait responses. Caged green sunfish induced much stronger non‐consumptive mortality than pirate perch in gray treefrogs, an effect that was absent for mole salamanders; golden topminnows had intermediate effects. Tail defenses were a function of prey mortality and only manifested in free‐roaming predator treatments, suggesting the necessity of predator attacks or conspecific alarm signals. Likewise, mole salamander polyphenism was unaffected by caged predators, whereas free‐roaming green sunfish prevented all metamorphosis. Free‐roaming golden topminnows increased the proportion of individuals remaining as larvae, and pirate perch increased the proportion of paedomorphs and metamorphs. Overall, the prey had positively correlated anti‐predator responses across life stages, suggesting multicomponent defenses. Predator effects varied with functional traits with large‐gaped predators having strong consumptive effects, but active predators causing stronger risk‐induced changes in growth compared to cryptic ambush predators, which provides more evidence for chemical camouflage. Our results emphasize the role of hunting mode and gape size in determining consumptive and predation‐risk effects, and that predation‐risk effects cannot be reliably predicted from consumptive effects.