Aerobic, heterotropic microorganisms of Dungeness crab (Cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. Each microbial isolate was then identified to the genus level employing the revised replica plating procedure. Microbial groups most commonly isolated from crab meat were, in the order of predominance, Moraxella, Pseudomonas, Acinetobacter, Arthrobacter, Micrococcus, Flavobacterium-Cytophaga, and Bacillussp. Proteus, Staphylococcus, yeasts, Vibrio, and Lactobacillussp. were found less frequently in some samples. Distribution patterns of microbial flora in crab meat revealed the presence of three classes of microorganisms. Microorganisms that originated from the raw crab and gained predominance by growth during refrigerated storage were Moraxella, Pseudomonas, Acinetobacter, and Flavobacterium-Cytophagasp. Those that originated from the crab but did not grow in meat were Arthrobacterand Bacillussp. Micrococcus, Staphylococcus, and Proteussp. were introduced during processing, but they did not grow in the refrigerated crab meat.
Pfeifer, D., Hirsch, H. J., Bergmann, D., and Hamlaoui, M.
Abstract
The physical distances between endpoints of deletions in the galoperon of λdgalphages previously isolated and mapped by genetic methods have been measured by electron microscopy of heteroduplex DNA. Thus, the galT and galK sites previously mapped by genetic methods, may be assigned to 13 and 4 intervals, respectively, of known physical lengths. The physical data clearly show a nonrandom distribution of these deletion endpoints.
Turbeville, J M, Pfeifer, D M, Field, K G, and Raff, R A
Abstract
Partial 18S rRNA sequences of five chelicerate arthropods plus a crustacean, myriapod, insect, chordate, echinoderm, annelid, and platyhelminth were compared. The sequence data were used to infer phylogeny by using a maximum-parsimony method, an evolutionary-distance method, and the evolutionary-parsimony method. The phylogenetic inferences generated by maximum-parsimony and distance methods support both monophyly of the Arthropoda and monophyly of the Chelicerata within the Arthropoda. These results are congruent with phylogenies based on rigorous cladistic analyses of morphological characters. Results support the inclusion of the Arthropoda within a spiralian or protostome coelomate clade that is the sister group of a deuterostome clade, refuting the hypothesis that the arthropods represent the "primitive" sister group of a protostome coelomate clade. Bootstrap analyses and consideration of all trees within 1% of the length of the most parsimonious tree suggest that relationships between the nonchelicerate arthropods and relationships within the chelicerate clade cannot be reliably inferred with the partial 18S rRNA sequence data. With the evolutionary-parsimony method, support for monophyly of the Arthropoda is found in the majority of the combinations analyzed if the coelomates are used as "outgroups." Monophyly of the Chelicerata is supported in most combinations assessed. Our analyses also indicate that the evolutionary-parsimony method, like distance and parsimony, may be biased by taxa with long branches. We suggest that a previous study's inference of the Arthropoda as paraphyletic may be the result of (a) having two few arthropod taxa available for analysis and (b) including long-branched taxa.
We present simple conditions under which Markov time changes are obtained, and give formulae for the resulting transition probabilities. [ABSTRACT FROM PUBLISHER]
Boztug, K., Jaervinen, P. M., Salzer, E., Racek, T., Moench, S., Garncarz, W., Gertz, E. M., Antonopoulos, A., Haslam, S., Schieck, L., Puchalka, J., Diestelhorst, J., Appaswamy, G., Lescoeur, B., Giambruno, R., Bigenzahn, J. W., Elling, U., Pfeifer, D., Welte, K., Brandes, G., Sherkat, R., Ten Bosch, J. Werff, Rezaei, N., Etzioni, A., Bellanne-Chantelot, C., Giulio Superti-Furga, Penninger, J. M., Bennett, K. L., Blume, J., Dell, A., Donadieu, J., and Klein, C.
Ivana Kolcic, Polašek, O., Pfeifer, D., Smolej-Narančić, N., Ilijić, M., Bljajić, D., Biloglav, Z., Ivanišević, M., and Delmiš, J.
Subjects
Male, Parity, Sex Factors, Croatia, Infant, Newborn, Birth Weight, Humans, Female, Gestational Age, newborns, anthropometrics, percentile curve, growth chart, Zagreb
Abstract
The aim of this study was to assess birth weight of healthy newborns from the City of Zagreb and Zagreb County, Croatia. Birth weights of healthy newborns, born at the Department of Obstetrics and Gynecology, University Hospital Center »Zagreb« in the year 2001, were included into analysis. Since there were only few newborns in the 22nd–27th week of gestation, they were excluded from the study. Small number of data points was also noticed in 28th–36th week of gestation, and was supplemented with the data from the years 2000, 2002 and 2003. The method of analysis used in this study was described by Altman and Chitty (Br. J. Obstet. Gynaecol., 101 (1994) 29). After the application of well defined exclusion criteria, the final sample consisted of 4,252 newborns. Percentile values for the four groups of newborns (male gender–primipara, male gender–multipara, female gender–primipara, female gender–multipara) were defined, yielding highest birth weight values in the male gender–multipara group (50th percentile of 40th gestational week was 3551.3 g), while female gender–primipara newborns were the lightest among the four sub-samples studied (50th percentile of 40th gestational week was 3399.9 g). New percentile values for percentile curves plotting are presented here and recommended for use in the clinical practice.
Zekan, J., Mario Kopljar, Pfeifer, D., and Pulanić, D.
Subjects
Low-birth-weight, Induced-abortion, Height, Age
Abstract
Recent observations that there is a secular variation in newborn weight and length call for their continuous assessment, as well as evaluating factors that influence them. The influence of maternal age, weight and height, as well as the number of previous deliveries, abortions and the number of cigarettes smoked per day on newborn weight and length was examined. Data were collected from 181 healthy pregnant women from Zagreb, Croatia, who delivered healthy newborns in term. Multiple regression, correlation coefficients and variance analysis were performed to assess the significance of tested variables on observed fetal features. Maternal age has no significant influence on birth weight, even though mothers younger than 20 and older than 30 tend to have lighter children. Both the numbers of previous deliveries and abortions showed no significant correlation to newborn weight. The number of cigarettes smoked per day during pregnancy and maternal pre-pregnancy weight were found to have significant correlation to newborn weight and length. Maternal height correlated significantly with newborn weight and length, but when multiple regression was performed, controlling for other parameters, no significant influence on newborn weight was found. These results indicate that smoking cessation and improvement in maternal nutritional status (expressed as body weight) are the two modifiable factors that play a significant role in the reduction of tow birth weight children, and thus the reduction of perinatal mortality.
Engelhardt, K. R., Mcghee, S., Winkler, S., Sassi, A., Woellner, C., Lopez-Herrera, G., Chen, A., Kim, H. S., Lloret, M. G., Schulze, I., Ehl, S., Thiel, J., Pfeifer, D., Veelken, H., Niehues, T., Siepermann, K., Weinspach, S., Reisli, I., Keles, S., Genel, F., Kutuculer, N., Camcioglu, Y., Somer, A., Karakoc-Aydiner, E., Barlan, I., Gennery, A., AYSE METIN, Degerliyurt, A., Pietrogrande, M. C., Yeganeh, M., Baz, Z., Al-Tamemi, S., Klein, C., Puck, J. M., Holland, S. M., Mccabe, E. R. B., Grimbacher, B., and Chatila, T. A.
Klaer, R., primary, Kuhn, S., additional, Fritz, H.-J., additional, Tillmann, E., additional, Saint-Girons, I., additional, Habermann, P., additional, Pfeifer, D., additional, and Starlinger, P., additional
Fischer, L., Deppert, W.R., Pfeifer, D., Stanzel, S., Weimer, M., Hanjalic-Beck, A., Stein, A., Straßer, M., Zahradnik, H.P., and Schaefer, W.R.
Subjects
*ENDOMETRIAL diseases, *EMBRYO implantation, *DRUG synergism, *TARGETED drug delivery, *PROGESTERONE receptors, *DRUG side effects, *DRUG development, *POLYMERASE chain reaction, *THERAPEUTICS
Abstract
Abstract: Embryo implantation is a crucial step in human reproduction and depends on the timely development of a receptive endometrium. The human endometrium is unique among adult tissues due to its dynamic alterations during each menstrual cycle. It hosts the implantation process which is governed by progesterone, whereas 17β-estradiol regulates the preceding proliferation of the endometrium. The receptors for both steroids are targets for drugs and endocrine disrupting chemicals. Chemicals with unwanted antigestagenic actions are potentially hazardous to embryo implantation since many pharmaceutical antiprogestins adversely affect endometrial receptivity. This risk can be addressed by human tissue-specific in vitro assays. As working basis we compiled data on chemicals interacting with the PR. In our experimental work, we developed a flexible in vitro model based on human endometrial Ishikawa cells. Effects of antiprogestin compounds on pre-selected target genes were characterized by sigmoidal concentration–response curves obtained by RT-qPCR. The estrogen sulfotransferase (SULT1E1) was identified as the most responsive target gene by microarray analysis. The agonistic effect of progesterone on SULT1E1 mRNA was concentration-dependently antagonized by RU486 (mifepristone) and ZK137316 and, with lower potency, by 4-nonylphenol, bisphenol A and apigenin. The negative control methyl acetoacetate showed no effect. The effects of progesterone and RU486 were confirmed on the protein level by Western blotting. We demonstrated proof of principle that our Ishikawa model is suitable to study quantitatively effects of antiprogestin-like chemicals on endometrial target genes in comparison to pharmaceutical reference compounds. This test is useful for hazard identification and may contribute to reduce animal studies. [Copyright &y& Elsevier]
The role of peroxisome proliferator-activated receptor-β/δ (PPAR-β/δ) in the pathogenesis of colon cancer remains highly controversial. This study specifically silenced the PPAR-β expression in three colon cancer cell lines with different metastatic potentials. Although PPAR-β knockdown resulted in more malignant morphological changes, bigger colony sizes and lower carcinoembryonic antigen (CEA) secretion, and enhanced the cell–fibronectin adhesion, cell invasion and migration were unaffected. These effects were stronger in poorly metastatic cell lines compared with highly metastatic ones. Simultaneously, PPAR-β knockdown decreased the mRNAs encoding adipocyte differentiation-related protein and liver fatty acid binding protein, and increased the mRNA of ILK, whereas the mRNAs encoding integrin-β1 and angiopoietin-like 4 were unchanged. Using immunohistochemistry, we determined that the intensity of PPAR-β expression was stronger in rectal cancers with better differentiation than in those with poor differentiation, and was stronger in early-stage tumors than in advanced ones. Together, these findings consistently indicate that PPAR-β may facilitate differentiation and inhibit the cell–fibronectin adhesion of colon cancer, having a role as an inhibitor in the carcinogenesis and progression of colorectal cancer. Interestingly, PPAR-β seems to have a more important role in poorly metastatic cells than in highly metastatic ones. [ABSTRACT FROM AUTHOR]
Due to the rapid worsening of the clinical situation with massive abdominal pain, we initiated therapy with anti-CD22 targeting Inotuzumab-ozogamicin (InO) (0.8 mg/m SP 2 sp d1 and 0.5 mg/m SP 2 sp d8) on day 11 after CAR-T-cell therapy. HT
Date
Material
CAR-T vector copy number
GAPDH-copies
CAR-T positive cells*
Proportion of CAR-T-cells of WBC [in %]**
CAR-T cell FACS [%]
04.05.2020
PB
6 Despite major recent advances in the therapy of aggressive lymphoma, patients with high-grade B-cell lymphoma, NOS with features intermediate between DLBCL and Burkitt lymphoma (BL) exhibit a poor prognosis compared to DLBCL or BL patients. [Extracted from the article]
Becker, M., Beyer, M., Dürr, M., Feier-Riesen, C., Förtsch, J., Gessler, T., Haas, R., Höhne, C., Kampert, K.-H., Lebedev, S., Michel, J., Otto, J.-H., Ovcharenko, E., Pauly, C., Pfeifer, D., Skott, P., Subramani, P., Traxler, M., and Weber, A.A.
Subjects
*DETECTORS, *PROTOTYPES, *MASS production, *TEST systems, *COOLING systems
Abstract
The Compressed Baryonic Matter (CBM) experiment is being built at the future Facility for Antiproton and Ion Research (FAIR) next to GSI, Darmstadt, Germany. A RICH detector will provide clean and efficient electron identification in order to access di-electrons emitted from dense baryonic matter, one of the key measurements of CBM. The CBM RICH recently passed the threshold to construction with first mass production of part of the electronics. The readout electronics was developed together with the HADES experiment and is successfully operated there in the upgraded RICH detector which also makes use of approximately half of the H12700 MAPMTs of CBM. The readout electronics is also tested in a dedicated "mini-RICH" detector in the "mini-CBM" setup with prototypes of all subdetectors, however with an implementation of the full functionality of the future free-streaming readout. Besides finalizing the RICH readout, work is ongoing on full size prototypes of the RICH mirror mechanics and the photocamera including a full system test of the cooling concept. In this report, a brief update on the various developments and the status of the CBM RICH detector is given. • CBM RICH passed threshold to construction. • DiRICH based readout chain commonly developed with HADES and validated. • full size photocamera prototype including closed-loop air-cooling. • free-streaming readout developed and validated. [ABSTRACT FROM AUTHOR]
Becker, M., Dürr, M., Faul, M., Förtsch, J., Friese, J., Höhne, C., Kampert, K.-H., Lebedev, S., Michel, J., Otto, J.-H., Patel, V., Pauly, C., Pfeifer, D., Schwab, E., Traxler, M., and Weber, A.A.
Subjects
*HEAVY-ion atom collisions, *PHOTOMULTIPLIERS, *HADRONS, *CHERENKOV counters, *HEAVY ion collisions
Abstract
The High Acceptance Dielectron Spectrometer (HADES) situated at GSI Darmstadt, Germany, has recently upgraded its gaseous ring imaging Cherenkov (RICH) detector. Studying the properties of the hot and dense fireballs produced in heavy ion collisions the RICH is the key component to efficiently identify electrons and positrons emitted therein. The new photon detection plane is comprised of 428 multianode photo electron multipliers (MAPMT)(Hamamatsu H12700) partly coated with p-Terphenyl as a wavelength shifter. It is operated with a gaseous C 4 H 10 (isobutane) radiator making it essentially hadron blind for particle momenta up to approximately 2.5 GeV/c. All 27392 MAPMT channels are read out by an FPGA based readout electronic scheme called DIRICH, which will also be used in other future Cherenkov detectors. The DIRICH readout allows to measure leading and trailing edges for each pixel pulse and hence time over threshold and hit arrival time down to sub-nanosecond precision. This article will discuss the key features of the upgraded HADES RICH and its performance based on a Ag+Ag run at E = 1. 58 A GeV incident energy within the FAIR Phase-0 research program. • HADES RICH upgraded with 428 Hamamatsu H12700 MAPMTs. • 200 ps timing precision reachable using DIRICH readout chain for MAPMT readout. • More than 17 hits per ring reached on average over full detector. • Detector integrated average of less than 15 background hits per event. [ABSTRACT FROM AUTHOR]
Becker, M., Beyer, M., Dürr, M., Feier-Riesen, C., Förtsch, J., Gessler, T., Haas, R., Höhne, C., Kampert, K.-H., Lebedev, S., Michel, J., Otto, J.-H., Ovcharenko, E., Pauly, C., Pfeifer, D., Skott, P., Subramani, P., Traxler, M., and Weber, A.A.
Subjects
*CONSTANT current sources, *PIXELS, *LIGHT sources, *PULSED lasers, *PHOTOMULTIPLIERS
Abstract
The DIRICH front-end board (FEB) is a 32+1 channel FPGA based TDC readout module for Multianode Photomultipliers (MAPMT), and also Multianode MCPs. Due to its low cost and excellent timing precision in single photon measurement applications it will be used in the RICH detector of the Compressed Baryonic Matter (CBM) experiment at the future FAIR facility at GSI Darmstadt, Germany. In the regions of highest photon density at the CBM RICH one expects an average hit rate of ≃ 300 kHz per MAPMT pixel (6 mm × 6 mm) and ∼ 15 % occupancy for minimum bias Au+Au collisions at 35 A GeV at an interaction rate of 10 MHz. In order to validate the high-rate capability of the DIRICH readout, a dedicated lab setup simulating realistic detector signals by employing a pulsed picosecond laser light source in combination with a constant current driven LED was commissioned. In this paper the setup is introduced and first results are discussed. We show that individual readout channels can withstand photon rates up to 2.2 MHz per pixel, limited only by maximum data rate capability and buffer size on the front-end board. Using the same setup, also effects of high photon occupancy on the MAPMTs are investigated, which might cause additional signals due to capacitive cross-talk within the MAPMT or readout chain. Occupancies of up to 55% (simultaneous photon hits on more than half of the MAPMT pixels) are investigated, indicating that in the expected occupancy range of 10 % – 15 % the readout works flawlessly with very low cross-talk. [ABSTRACT FROM AUTHOR]
Dederichs, T.-S., Ehlert, C., Becker, H., Pfeifer, D., Niemoeller, C., Anto-Michel, N., Zirlik, A., Bode, C., Wolf, D., Von Zur Mühlen, C., Kaier, K., Preissl, S., Heidt, T., Westermann, D., and Hilgendorf, I.
Pauly, C., Eschke, J., Faul, M., Friese, J., Höhne, C., Kampert, K.-H., Kunz, T., Lebedev, S., Michel, J., Niebur, W., Patel, V., Pfeifer, D., Skott, P., Traxler, M., Ugur, C., Weber, A., and Zumbruch, P.
Subjects
*PHOTON detectors, *HEAVY ion collisions, *PARTICLE accelerators, *CATHODES, *PHOTOMULTIPLIERS
Abstract
The HADES RICH detector is designed for efficient electron identification (electron momenta up to few GeV) in relativistic heavy ion collisions, and successfully in operation since 1999 at the SIS18 accelerator facility, GSI, Darmstadt, Germany. It uses a gaseous photon detector with reflective CsI cathode deposited on the MWPC pad plane. The CBM experiment at the future FAIR facility in Darmstadt will install a RICH detector utilizing 1100 Hamamatsu H12700 Multianode Photomultiplier tubes. In a joint effort the HADES RICH photon detector will be replaced by a subset of these MAPMTs together with a new FPGA-TDC based readout chain resulting in a significant improvement of e + e − - pair reconstruction efficiency for near future measurement campaigns. [ABSTRACT FROM AUTHOR]