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Summary: A high point of Tibetan Plateau (TP) civilization, the expansive Tubo Empire (618–842 AD) wielded great influence across ancient western China. However, whether the Tubo expansion was cultural or demic remains unclear due to sparse ancient DNA sampling. Here, we reported ten ancient genomes at 0.017- to 0.867-fold coverages from the Dulan site with typical Tubo archaeological culture dating to 1308–1130 BP. Nine individuals from three different grave types have close relationship with previously reported ancient highlanders from the southwestern Himalayas and modern core-Tibetan populations. A Dulan-related Tubo ancestry contributed overwhelmingly (95%–100%) to the formation of modern Tibetans. A genetic outlier with dominant Eurasian steppe-related ancestry suggesting a potential population movement into the Tubo-controlled regions from Central Asia. Together with archeological evidence from burial styles and customs, our study suggested the impact of the Tubo empire on the northeast edge of the TP involved both cultural and demic diffusion.

Summary: The cheetah Acinonyx jubatus, the fastest living land mammal, is an atypical member of the family Felidae. The extinct feline Miracinonyx trumani, known as the North American cheetah, is thought to have convergently evolved with Acinonyx to pursue fast and open-country prey across prairies and steppe environments of the North American Pleistocene. The brain of Acinonyx is unique among the living felids, but it is unknown whether the brain of the extinct M. trumani is convergent to that of Acinonyx. Here, we investigate the brain of M. trumani from a cranium endocast, using a comparative sample of other big cats. We demonstrate that the brain of M. trumani was different from that of the living A. jubatus. Indeed, its brain shows a unique combination of traits among living cats. This suggests that the case of extreme convergence between Miracinonyx and its living Old World vicar should be reconsidered.

Summary: Archaeological research shows that the dispersal of the Neolithic took a more complex turn when reaching western Europe, painting a contrasted picture of interactions between autochthonous hunter-gatherers (HGs) and incoming farmers. In order to clarify the mode, the intensity, and the regional variability of biological exchanges implied in these processes, we report new palaeogenomic data from Occitanie, a key region in Southern France. Genomic data from 28 individuals originating from six sites spanning from c. 5,500 to c. 2,500 BCE allow us to characterize regional patterns of ancestries throughout the Neolithic period. Results highlight major differences between the Mediterranean and Continental Neolithic expansion routes regarding both migration and interaction processes. High proportions of HG ancestry in both Early and Late Neolithic groups in Southern France support multiple pulses of inter-group gene flow throughout time and space and confirm the need for regional studies to address the complexity of the processes involved.

Summary: Canarium L. contains approximately 78 species distributed in low to middle altitudes of the Paleotropics and northern Australia. Canarium fruit fossils are known mainly from Paleogene to Neogene of North America, Africa, and Eurasia. Here, we described a new species Canarium maomingense sp. nov. from the upper Pleistocene of the Maoming Basin, Guangdong, South China. Similarly to extant Canarium species, each of three locules of C. maomingense possesses two ovules, but only one or two of six ovules develop into a seed, indicating that the ovules undeveloped into seeds in Canarium species have existed at least since the late Pleistocene. The natural habitats of extant relatives and associated fossil plants suggest subtropical evergreen broad-leaved and mixed forests in the late Pleistocene of this region. Some special damage traces are observed on pyrene surfaces, indicating possible plant interactions with animals and fungi.

Summary: Nurse logs are common in modern forests from boreal to temperate and tropical ecosystems. However, the evolution of the nurse-log strategy remains elusive because of their rare occurrence in the fossil record. We report seven coniferous nurse logs from lowermost to uppermost Permian strata of northern China that have been colonized by conifer and sphenophyllalean roots. These roots are associated with two types of arthropod coprolites and fungal remains. Our study provides the first glimpse into plant—plant facilitative relationships between late Paleozoic gymnosperms and sphenopsids. Detritivorous arthropods and fungi appear to have been crucial for the utilization of nurse logs in Permian forests. The phylogenetically distant roots demonstrate that nurse-log interaction was a sophisticated seedling strategy in late Paleozoic humid tropical forests, and this approach may have been adopted and developed by a succession of plant groups leading to its wide representation in modern forest ecosystems.

Simple Summary: Numerous prehistoric sites in Europe and the Near East provided bones and dental remains of the populations of the past. One of them is the Combe-Grenal Cave (SW France), where fossils of children and adults represent the Neanderthals who lived there more than 60 ky ago, during a harsh period of the last glaciation. In this paper, we analyze a sample of the tartar of a juvenile individual. The numerous bacteria forming the plaque are compared to those of one adult from Israel, Kebara 2, revealing the differences between the most common bacteria in a young and an older individual, probably because of their immunological systems, and the different living conditions of the human groups they represented. Combe-Grenal site (Southwest France) was excavated by F. Bordes between 1953 and 1965. He found several human remains in Mousterian levels 60, 39, 35 and especially 25, corresponding to MIS 4 (~75–70/60 ky BP) and with Quina Mousterian lithics. One of the fossils found in level 25 is Combe-Grenal IV, consisting of a fragment of the left corpus of a juvenile mandible. This fragment displays initial juvenile periodontitis, and the two preserved teeth (LLP4 and LLM1) show moderate attrition and dental calculus. The SEM tartar analysis demonstrates the presence of cocci and filamentous types of bacteria, the former being more prevalent. This result is quite different from those obtained for the two adult Neanderthals Kebara 2 and Subalyuk 1, where more filamentous bacteria appear, especially in the Subalyuk 1 sample from Central Europe. These findings agree with the available biomedical data on periodontitis and tartar development in extant individuals, despite the different environmental conditions and diets documented by numerous archeological, taphonomical and geological data available on Neanderthals and present-day populations. New metagenomic analyses are extending this information, and despite the inherent difficulties, they will open important perspectives in studying this ancient human pathology. [ABSTRACT FROM AUTHOR]

The Late Jurassic, 'single surface' Moab Megatracksite between Green River, Utah and the Colorado-Utah State line is associated with the sharp, colour-enhanced, inter-formational contact between white, reworked dune sandstones from the aeolian Moab Member of the Curtis Formation and red, sabkha facies siltstones and sandstones of the upper tongue of the Summerville Formation. Previous studies described theropod (Megalosauripus and Therangospodus) dominated assemblages from ~25 sites in the megatracksite core area around Arches National Park, and briefly interpreted the transgressive mechanisms of the Sundance sea which helped create and preserve the megatracksite. Since the 1990s ~20 newly studied tracksites reveal a cumulative total of ~4,300 tracks and three sites are now designated as, federally managed, interpretative tourist destinations. Similar track assemblages, collectively constituting Megalosauripus-Therangospodus ichnocoenoses have now also been found at lower stratigraphical levels associated with J-3 Unconformity contact zones. These new discoveries relate to stratigraphic studies associated with short-term changes in Sundance Sea level affecting coastal dune facies (Slick Rock and Moab members) across low gradient, inter- and sub-tidal sand flats, and sabkha-like deposits under arid conditions. The multiple (>50) theropod dominated ichnofaunas are integral to palaeobiological understanding of an area from which no body fossils are known. [ABSTRACT FROM AUTHOR]

The definition of the Cretaceous shark genus Cretodus Sokolov, 1965 is primarily based on isolated teeth. This genus includes five species. Among these, Cretodus houghtonorum Shimada and Everhart, 2019 is the only species based on a partially preserved skeleton. Here, the taxonomic attribution of a virtually complete skeleton of Cretodus from the Turonian of northeastern Italy is discussed, together with a few specimens from the Turonian of England. One of the latter is investigated through micropaleontological analysis to determine its stratigraphic position. The material is referred to Cretodus crassidens (Dixon, 1850), the diagnosis of which is emended herein. The dentition is tentatively reconstructed, exhibiting strong similarities with congeneric species, although it differs in having strong vertical folds on the main cusp labial face, a mesiodistally broad tooth aspect, weak and well-spaced 'costulae' at crown base, and a different dental formula in the number of parasymphyseal and lateral rows. Some tooth malformations are interpreted as feeding-related or senile characters. The Italian specimen suggests that Cretodus crassidens had a wide and laterally expanded mouth and head, a stout body, and attained a gigantic size. Cretodus crassidens was a moderate-speed swimming shark ecologically like the extant tiger shark Galeocerdo cuvier (Péron and Lesueur in Lesueur, 1822). The age estimate from vertebral-band counting suggests that the Italian individual was at least 23 years old and the growth model indicates a longevity of 64 years and a maximum attainable total length of 9–11 m. Cretodus crassidens occurs both in Boreal and Tethyan domains, implying a broad paleobiogeographic distribution and a preference toward offshore settings. [ABSTRACT FROM AUTHOR]

Evolvability is best addressed from a multi-level, macroevolutionary perspective through a comparative approach that tests for among-clade differences in phenotypic diversification in response to an opportunity, such as encountered after a mass extinction, entering a new adaptive zone, or entering a new geographic area. Analyzing the dynamics of clades under similar environmental conditions can (partially) factor out shared external drivers to recognize intrinsic differences in evolvability, aiming for a macroevolutionary analog of a common-garden experiment. Analyses will be most powerful when integrating neontological and paleontological data: determining differences among extant populations that can be hypothesized to generate large-scale, long-term contrasts in evolvability among clades; or observing large-scale differences among clade histories that can by hypothesized to reflect contrasts in genetics and development observed directly in extant populations. However, many comparative analyses can be informative on their own, as explored in this overview. Differences in clade-level evolvability can be visualized in diversity-disparity plots, which can quantify positive and negative departures of phenotypic productivity from stochastic expectations scaled to taxonomic diversification. Factors that evidently can promote evolvability include modularity—when selection aligns with modular structure or with morphological integration patterns; pronounced ontogenetic changes in morphology, as in allometry or multiphase life cycles; genome size; and a variety of evolutionary novelties, which can also be evaluated using macroevolutionary lags between the acquisition of a trait and phenotypic diversification, and dead-clade-walking patterns that may signal a loss of evolvability when extrinsic factors can be excluded. High speciation rates may indirectly foster phenotypic evolvability, and vice versa. Mechanisms are controversial, but clade evolvability may be higher in the Cambrian, and possibly early in the history of clades at other times; in the tropics; and, for marine organisms, in shallow-water disturbed habitats. [ABSTRACT FROM AUTHOR]

Simple Summary: All life is derived from a single common ancestor, whose descendants coevolved with the planet, shaping the structure of biodiversity and the physical processes that operate on Earth. This complex history cannot be inferred solely by studying the genomes of living organisms, nor through analysis of the fossil remains of their extinct relatives. Only a unified approach integrating living and extinct species and drawing from both genomic and anatomical evidence can achieve this aim. In this review, we highlight recent advances, challenges, and opportunities in this endeavour. These include the development of models for analysis of anatomical data; methods for combined analysis of fossil and living species, as well as anatomical and genomic data; and the combined estimation of evolutionary relationships, geographic range, and evolutionary rates. However, the application of such methods is limited by a shortage of expertise in taxonomy and comparative anatomy, which are skills required for the compilation of anatomical datasets. Whereas there is a common concern for the incompleteness of the fossil record, knowledge with respect to the comparative anatomy of living species is equally incomplete. We anticipate that the increased demand for an integrative phylogenetic approach to reconstruct the tree of life and evolutionary patterns and processes will encourage researchers to overcome these challenges with the aim of elucidating the complexities behind organismal evolution across broad taxonomic and time scales. The modern era of analytical and quantitative palaeobiology has only just begun, integrating methods such as morphological and molecular phylogenetics and divergence time estimation, as well as phenotypic and molecular rates of evolution. Calibrating the tree of life to geological time is at the nexus of many disparate disciplines, from palaeontology to molecular systematics and from geochronology to comparative genomics. Creating an evolutionary time scale of the major events that shaped biodiversity is key to all of these fields and draws from each of them. Different methodological approaches and data employed in various disciplines have traditionally made collaborative research efforts difficult among these disciplines. However, the development of new methods is bridging the historical gap between fields, providing a holistic perspective on organismal evolutionary history, integrating all of the available evidence from living and fossil species. Because phylogenies with only extant taxa do not contain enough information to either calibrate the tree of life or fully infer macroevolutionary dynamics, phylogenies should preferably include both extant and extinct taxa, which can only be achieved through the inclusion of phenotypic data. This integrative phylogenetic approach provides ample and novel opportunities for evolutionary biologists to benefit from palaeontological data to help establish an evolutionary time scale and to test core macroevolutionary hypotheses about the drivers of biological diversification across various dimensions of organisms. [ABSTRACT FROM AUTHOR]

Simple Summary: This article examines the relationship between twentieth- and twenty-first-century paleobiology. After summarizing the disciplinary problem of paleontology in the mid-twentieth century, I focus on five representative research topics in contemporary paleontology. In doing so, I outline twenty-first-century paleontology as a science that seeks more data, more technology, and more integration. At the end of the paper, I highlight a possible new paleobiological revolution: it would give paleontologists strong political representation to deal with issues such as expanded evolutionary synthesis, conservation of the Earth's environment, and global climate change. In this paper, I will briefly discuss the elements of novelty and continuity between twentieth-century paleobiology and twenty-first-century paleontology. First, I will outline the heated debate over the disciplinary status of paleontology in the mid-twentieth century. Second, I will analyze the main theoretical issue behind this debate by considering two prominent case studies within the broader paleobiology agenda. Third, I will turn to twenty-first century paleontology and address five representative research topics. In doing so, I will characterize twenty-first century paleontology as a science that strives for more data, more technology, and more integration. Finally, I will outline what twenty-first-century paleontology might inherit from twentieth-century paleobiology: the pursuit of and plea for a new synthesis that could lead to a second paleobiological revolution. Following in the footsteps of the paleobiological revolution of the 1960s and 1970s, the paleobiological revolution of the twenty-first century would enable paleontologists to gain strong political representation and argue with a decisive voice at the "high table" on issues such as the expanded evolutionary synthesis, the conservation of Earth's environment, and global climate change. [ABSTRACT FROM AUTHOR]

The invasion of the land was a complex, protracted process, punctuated by mass extinctions, that involved multiple routes from marine environments. We integrate paleobiology, ichnology, sedimentology, and geomorphology to reconstruct Paleozoic terrestrialization. Cambrian landscapes were dominated by laterally mobile rivers with unstable banks in the absence of significant vegetation. Temporary incursions by arthropods and worm-like organisms into coastal environments apparently did not result in establishment of continental communities. Contemporaneous lacustrine faunas may have been inhibited by limited nutrient delivery and high sediment loads. The Ordovician appearance of early land plants triggered a shift in the primary locus of the global clay mineral factory, increasing the amount of mudrock on the continents. The Silurian–Devonian rise of vascular land plants, including the first forests and extensive root systems, was instrumental in further retaining fine sediment on alluvial plains. These innovations led to increased architectural complexity of braided and meandering rivers. Landscape changes were synchronous with establishment of freshwater and terrestrial arthropod faunas in overbank areas, abandoned fluvial channels, lake margins, ephemeral lakes, and inland deserts. Silurian–Devonian lakes experienced improved nutrient availability, due to increased phosphate weathering and terrestrial humic matter. All these changes favoured frequent invasions to permament establishment of jawless and jawed fishes in freshwater habitats and the subsequent tetrapod colonization of the land. The Carboniferous saw rapid diversification of tetrapods, mostly linked to aquatic reproduction, and land plants, including gymnosperms. Deeper root systems promoted further riverbank stabilization, contributing to the rise of anabranching rivers and braided systems with vegetated islands. New lineages of aquatic insects developed and expanded novel feeding modes, including herbivory. Late Paleozoic soils commonly contain pervasive root and millipede traces. Lacustrine animal communities diversified, accompanied by increased food-web complexity and improved food delivery which may have favored permanent colonization of offshore and deep-water lake environments. These trends continued in the Permian, but progressive aridification favored formation of hypersaline lakes, which were stressful for colonization. The Capitanian and end-Permian extinctions affected lacustrine and fluvial biotas, particularly the invertebrate infauna, although burrowing may have allowed some tetrapods to survive associated global warming and increased aridification. [ABSTRACT FROM AUTHOR]

Symbioses between metazoans and microbes involved in sulfur cycling are integral to the ability of animals to thrive within deep‐sea hydrothermal vent environments; the development of such interactions is regarded as a key adaptation in enabling animals to successfully colonize vents. Microbes often colonize the surfaces of vent animals and, remarkably, these associations can also be observed intricately preserved by pyrite in the fossil record of vent environments, stretching back to the lower Paleozoic (Ordovician‐early Silurian). In non‐vent environments, sulfur isotopes are often employed to investigate the metabolic strategies of both modern and fossil organisms, as certain metabolic pathways of microbes, notably sulfate reduction, can produce large sulfur isotope fractionations. However, the sulfur isotopes of vent fossils, both ancient and recently mineralized, have seldom been explored, and it is not known if the pyrite‐preserved vent organisms might also preserve potential signatures of their metabolisms. Here, we use high‐resolution secondary ion mass spectrometry (SIMS) to investigate the sulfur isotopes of pyrites from recently mineralized and Ordovician‐early Silurian tubeworm fossils with associated microbial fossils. Our results demonstrate that pyrites containing microbial fossils consistently have significantly more negative δ34S values compared with nearby non‐fossiliferous pyrites, and thus represent the first indication that the presence of microbial sulfur‐cycling communities active at the time of pyrite formation influenced the sulfur isotope signatures of pyrite at hydrothermal vents. The observed depletions in δ34S are generally small in magnitude and are perhaps best explained by sulfur isotope fractionation through a combination of sulfur‐cycling processes carried out by vent microbes. These results highlight the potential for using sulfur isotopes to explore biological functional relationships within fossil vent communities, and to enhance understanding of how microbial and animal life has co‐evolved to colonize vents throughout geological time. [ABSTRACT FROM AUTHOR]

Francis M. Hueber was an important figure in mid- and late twentieth-century paleobotany; a specialist in Devonian plants, he focused his research on a much broader array of topics. Here we briefly describe his early life and education, outline his 40-year career at the Smithsonian National Museum of Natural History (NMNH) in Washington, DC, and review some of his most important publications, most significantly on the once-enigmatic Devonian fossil Prototaxites. We review his important field expeditions and contributions to the collections in the Department of Paleobiology of the NMNH and his educational activities at the NMNH. Finally, some personal reminiscences are presented. [ABSTRACT FROM AUTHOR]

Beothukis mistakensis from the Ediacaran System of Newfoundland, Canada demonstrates complex fractal-like morphology through the development of primary-, secondary- and tertiary-order Rangea-like units. The primary-order rangeomorph units observed in B. mistakensis are tightly juxtaposed, show no evidence of being independent of one another and are made up of chamber-like secondary-order – probably mesoglea-filled – units. The growth of these rangeomorph units demonstrates that the frond developed from the tip towards the basal region through ontogeny. The tertiary-order units of Beothukis are considered to represent surface morphology on the secondary-order units. This is in contrast to palaeobiological reconstructions of Beothukis that invoke three-dimensional fractal-like branches with independent units, which has been used to infer an osmotrophic mode of life. It is considered here that the fractal-like morphology of the lower surface of B. mistakensis was an adaptation to increase surface area to volume ratio. The quilted morphology of Beothukis proposed here is consistent with a sessile, reclining, phagocytotic and/or chemosymbiotic mode of life similar to that invoked for the reclining rangeomorph Fractofusus. [ABSTRACT FROM AUTHOR]

We present a detailed histological study of long bones from an ontogenetic series of Mussaurus patagonicus, an early sauropodomorph from the Lower Jurassic of Argentina. Twenty long bones, including humeri, femora and fibulae, obtained from 13 individuals of different body sizes were sampled for histological analysis. In general terms, the cortical bone is formed by a well vascularized fibrolamellar and parallel fibred bone. Except for the smaller individuals, cyclical growth marks (CGMs) are well recorded in all the specimens but their number and relative position is highly variable. Mussaurus exhibits marked variation regarding relative growth rate, with some individuals growing much faster than others. Such variation affects the size of the adult individuals, which results in a poor correlation between the body size and the age/ontogenetic stage for this taxon. These discrepancies may be related to sexual dimorphism and/or developmental plasticity. Intraspecific variation is also recorded with regard to the growth strategies, which can vary from cyclical, as in other early sauropodomorphs, to continuous, as reported in sauropods. Sexual maturity appears to have been reached between 23 and 31 years, which is delayed in comparison to other early sauropodomorphs but more comparable with derived sauropods. The attainment of somatic maturity appears to have been reached at about 14 years after onset of sexual maturity. Mussaurus is a sauropodiform, phylogenetically closer to sauropods than most other Early Jurassic sauropodomorphs, and therefore provides critical information for understanding palaeobiological aspects of the origin of sauropods and the onset of gigantism in this lineage. [ABSTRACT FROM AUTHOR]

Micropalaeontological studies can provide important information on how strata correlate, age calibration, palaeoenvironmental determination, palaeobiology, evolutionary relationships of the taxa being studied, palaeogeography, and the recognition of global or regional geological events in a local succession. However, the value of micropalaeontological studies is constrained by the accuracy with which taxa are identified, the accuracy of their age interpretation, and the use of up-to-date taxonomic concepts, supported by modern research literature. Best practice implies attention to these and other details, which we illustrate with reference to published research on larger benthic foraminifera (especially orbitolinids) from the mid-Cretaceous succession of the Zagros Mountains in the Middle East. It is demonstrated that whilst there are many excellent studies, a significant number could offer more value if they were to use precise, modern taxonomic concepts applied to well-illustrated and documented unequivocal material. Poorly founded age assignments and misidentifications can lead to confusion on assessment of true stratigraphic ranges and evolutionary patterns. Moreover, they can lead to miscorrelation and erroneous modelling of the subsurface in a hydrocarbon-rich region. Integrated studies of larger benthic foraminifera with other age-diagnostic fossil groups or chemostratigraphic methods would further enhance their utility. These recommendations are applicable to micropalaeontological studies on microfossils of any type and age, from any part of the world, but we hope will promote additional rigour in studies on the micropalaeontology of the Mesozoic and Cenozoic succession of the Zagros that still have much to impart. [ABSTRACT FROM AUTHOR]

Tyrannosauroids are a clade of theropod dinosaur taxa that varied greatly in their body size distribution. We investigated the feeding performance of six tyrannosaur genera of variable body size and skull morphology. We used 3D finite element analysis to test whether skull shape becomes more or less resistant to feeding-induced forces. Cranial and mandibular models were scaled by adult Tyrannosaurus 's surface area to analyze the influence of shape on skull function. It was found that Tyrannosaurus experienced higher absolute stresses compared to small-bodied relatives. When surface area values were equalized across genera to account for the effect of size and test efficiency of skull shape, smaller individuals experience notably greater stresses than larger relatives due to the robust cranial osteology characterized in the allometry of tyrannosaurids. These results may indicate that the wide crania of tyrannosaurids convey a functional advantage that basal tyrannosauroids, juvenile tyrannosauroids, and alioramins lacked., Competing Interests: The authors declare no competing interests., (Crown Copyright © 2024 Published by Elsevier Inc.)

Summary: Ailanthus Desf. (Simaroubaceae), now widespread in southern Asia to northern Australia, was widely distributed in the Northern Hemisphere during the Cenozoic, but has few fossil records at low latitudes. Here we report the fossil samaras of Ailanthus confucii Unger from South China and its occurrences indicate that this genus has been distributed in low latitude regions since the middle Eocene. According to the recent fossil records, Ailanthus is considered to have originated from the Indian subcontinent and dispersed rapidly to East Asia and western North America following the early Paleogene onset of the India-Eurasia collision. In the Eocene, Ailanthus became widespread across the Northern Hemisphere. Subsequent to global cooling, Ailanthus gradually disappeared in the mid-high latitudes and may have continued to spread southward from Asia to northern Australia following the Asia-Australia collision in the late Oligocene, thus forming its modern distribution pattern.

Summary: The origin of mandibulate arthropods can be traced back to the Cambrian period to several carapace-bearing arthropod groups, but their morphological diversity is still not well characterized. Here, we describe Balhuticaris voltae, a bivalved arthropod from the 506-million-year-old Burgess Shale (Marble Canyon, British Columbia, Canada). This species has an extremely elongated and multisegmented body bearing ca. 110 pairs of homonomous biramous limbs, the highest number among Cambrian arthropods, and, at 245 mm, it represents one of the largest Cambrian arthropods known. Its unusual carapace resembles an arch; it covers only the frontalmost section of the body but extends ventrally beyond the legs. Balhuticaris had a complex sensory system and was probably an active swimmer thanks to its powerful paddle-shaped exopods and a long and flexible body. Balhuticaris increases the ecological and functional diversity of bivalved arthropods and suggests that cases of gigantism occurred in more arthropod groups than previously recognized.

This article discusses the concept of Virtual Paleobiology and its applications in the field of paleontology. The use of X-ray microcomputed tomography (μCT) allows for the virtual representation of fossils, enhancing paleontologists' ability to study and understand the biology, evolution, and ecology of organisms. The article presents various research studies that utilize μCT to analyze marine and terrestrial fossils, including measurements of planktic foraminiferal tests, changes in foraminifera calcification, biostratigraphic analysis of conodont microfossils, and morphological analysis of fossil plants and animal skulls. The article concludes by highlighting the potential of μCT in creating online databases and expressing gratitude to the authors and reviewers who contributed to the research. [Extracted from the article]

Hydrothermal systems and their deposits are primary targets in the search for fossil evidence of life beyond Earth. However, to learn how to decode fossil biomarker records in ancient hydrothermal deposits, we must first be able to interpret unambiguously modern biosignatures, their distribution patterns, and their association with physicochemical factors. Here, we investigated the molecular and isotopic profile of microbial biomarkers along a thermal gradient (from 29 to 72°C) in a hot spring (labeled Cacao) from El Tatio, a geyser field in the Chilean Andes with abundant opaline silica deposits resembling the nodular and digitate structures discovered on Mars. As a molecular forensic approach, we focused on the analysis of lipid compounds bearing recognized resistance to degradation and the potential to reconstruct the paleobiology of an environment on a broader temporal scale than other, more labile, biomolecules. By exploiting the lipid biomarkers' potential to diagnose biological sources and carbon fixation pathways, we reconstructed the microbial community structure and its ecology along the Cacao hydrothermal transect. The taxonomic adscription of the lipid biomarkers was qualitatively corroborated with DNA sequencing analysis. The forensic capacity of the lipid biomarkers to identify biosources in fresh biofilms was validated down to the genus level for Roseiflexus , Chloroflexus , and Fischerella. We identified lipid biomarkers and DNA of several new cyanobacterial species in El Tatio and reported the first detection of Fischerella biomarkers at a temperature as high as 72°C. This, together with ecological peculiarities and the proportion of clades being characterized as unclassified, illustrates the ecological singularity of El Tatio and strengthens its astrobiological relevance. The Cacao hydrothermal ecosystem was defined by a succession of microbial communities and metabolic traits associated with a high- (72°C) to low-(29°C) temperature gradient that resembled the inferred metabolic sequence events from the 16S rRNA gene universal phylogenetic tree from thermophilic to anoxygenic photosynthetic species and oxygenic phototrophs. The locally calibrated DNA-validated lipidic profile in the Cacao biofilms provided a modern (molecular and isotopic) end member to facilitate the recognition of past biosources and metabolisms from altered biomarkers records in ancient silica deposits at El Tatio analogous to Martian opaline silica structures. [ABSTRACT FROM AUTHOR]

Ecological preferences and life history strategies have enormous impacts on the evolution and phenotypic diversity of salamanders, but the yet established reliable ecological indicators from bony skeletons hinder investigations into the paleobiology of early salamanders. Here, we statistically demonstrate by using time-calibrated cladograms and geometric morphometric analysis on 71 specimens in 36 species, that both the shape of the palate and many non-shape covariates particularly associated with vomerine teeth are ecologically informative in early stem-and basal crown-group salamanders. Disparity patterns within the morphospace of the palate in ecological preferences, life history strategies, and taxonomic affiliations were analyzed in detail, and evolutionary rates and ancestral states of the palate were reconstructed. Our results show that the palate is heavily impacted by convergence constrained by feeding mechanisms and also exhibits clear stepwise evolutionary patterns with alternative phenotypic configurations to cope with similar functional demand. Salamanders are diversified ecologically before the Middle Jurassic and achieved all their present ecological preferences in the Early Cretaceous. Our results reveal that the last common ancestor of all salamanders share with other modern amphibians a unified biphasic ecological preference, and metamorphosis is significant in the expansion of ecomorphospace of the palate in early salamanders. [ABSTRACT FROM AUTHOR]

Ultraviolet (UV)-screening compounds represent a substantial asset for the survival of cyanobacteria in extreme environments exposed to high doses of UV radiations on modern and early Earth. Among these molecules, the halochromic pigment gloeocapsin remains poorly characterized and studied. In this study, we identified a gloeocapsin-producing cultivable cyanobacteria: the strain Phormidesmis nigrescens ULC007. We succeeded to extract, to partially purify, and to compare the dark blue pigment from both the ULC007 culture and an environmental Gloeocapsa alpina dominated sample. FT-IR and Raman spectra of G. alpina and P. nigrescens ULC007 pigment extracts strongly suggested a common backbone structure. The high-pressure liquid chromatography-UV-MS/MS analysis of the ULC007 pigment extract allowed to narrow down the molecular formula of gloeocapsin to potentially five candidates within three classes of halochromic molecules: anthraquinone derivatives, coumarin derivatives, and flavonoids. With the discovery of gloeocapsin in P. nigrescens, the production of this pigment is now established for three lineages of cyanobacteria (including G. alpina, P. nigrescens, and Solentia paulocellulare) that belong to three distinct orders (Chroococcales, Pleurocapsales, Synechoccocales), inhabiting very diverse environments. This suggests that gloeocapsin production was a trait of their common ancestor or was acquired by lateral gene transfer. This work represents an important step toward the elucidation of the structure of this enigmatic pigment and its biosynthesis, and it potentially provides a new biosignature for ancient cyanobacteria. It also gives a glimpse on the evolution of UV protection strategies, which are relevant for early phototrophic life on Earth and possibly beyond. [ABSTRACT FROM AUTHOR]

Sedimentological, paleobiological, mineralogical, and geochemical analyses of a sediment core retrieved on the seafront of Cartagena Bay were performed after high-resolution sampling. A wide series of dates obtained through radiocarbon and amino acid racemization proved that the Holocene record on the analyzed core began at ca. 7300 yr cal BP. Reinterpretation of the marine seismic profiles indicated that the beginning of this transgression was channeled along erosive paleochannels excavated on a coastal plain of, at least, MIS5c age. The Holocene paleoenvironmental evolution consisted of a first period marked by marine conditions with strong inputs from a fan delta linked to the reorganized fluvial network that occurred after the sudden rise of the base level, which caused a growing sedimentary prism. Later, the full marine environment was reflected in the development of a Posidonia - Cymodocea meadow hosting a rich biodiversity of marine species (mollusks, foraminifera, ostracoda). Around 3800 yr cal BP, the area underwent a profound change and a silting process started to alter the conditions, turning the area into a paucispecific brackish marsh environment in which mud deposition was dominant. Since Carthaginian times, arrangement works locally affected the record, allowing the arrival of some marine species due to port work (dredging) undertaken to gain depth and showing anthropic influence. [ABSTRACT FROM AUTHOR]

Body mass estimations for extinct taxa are fundamental in palaeobiological reconstructions, but little work has been done on this topic for non-mammaliaform cynodonts (NMC), the diverse and abundant Permo-Cretaceous forerunners of mammals. Here, we estimated the body mass of five species of NMC cynognathians by linear measurements and circumferences of postcranial elements (humeri and femora) from 14 specimens from Triassic units of the Ischigualasto-Villa Union Basin located between San Juan and La Rioja provinces, and the Cuyo and San Rafael basins, both in Mendoza province, Argentina. For this purpose, we used predictive formulas available in the literature based on variables on appendicular skeleton of different extant groups of mammals and reptiles. Geometric similarity using skull length was applied to provide an estimation of adult masses for species with only samples of juvenile and subadult limb bones. A broad body mass range was recorded. Small traversodontids such as Andescynodon mendozensis and Pascualgnathus polanskii were between 1 to 3.5 kg. Medium-sized traversodontids include adult Massetognathus pascuali with masses from 20 to 40 kg, and the adult forms of large-sized cynognathians like Cynognathus crateronotus and Exaeretodon argentinus reached or surpassed 100 kg. The morphological variations in the skull and the different body sizes observed between traversodontids are interpreted as reflecting different types of diets where small-sized traversodontids had a generalist diet, and the medium/large-sized traversodontids were herbivorous. Finally, palaeoecological working hypotheses regarding cranial and dental morphology, body mass, and their possible relation with diet in non-mammaliaform cynodonts of South America are offered. [ABSTRACT FROM AUTHOR]

Understanding the phylogenetic relationship among derived blastozoans has been a goal of researchers since phylogenetic methodologies were first applied to Paleozoic echinoderms. Paul (2021) proposed a new "pan-dichoporites" group to circumscribe early Paleozoic blastozoans. Unfortunately, this work includes many inaccuracies, non-reproducible analyses, and nonstandard method choices that confuse rather than advance the understanding of echinoderm paleobiology. Herein, we focus on key aspects of philosophy, methodology, and data reproducibility the publication of Paul (2021) raises that need to be addressed and considered by echinoderm researchers as they assess the concept of pan-dichoporite echinoderms. [ABSTRACT FROM AUTHOR]

We document an upper upper Albian (Mortoniceras rostratum Zone) cephalopod assemblage from Clansayes (Drôme, south-eastern France). Although fossils are rare in local exposures and in the single sampled level, a decade of intensive fossil collecting yielded 290 ammonite and 5 nautilid specimens. In total, we describe 1 species of nautilid and 24 species (within 17 genera) of ammonites, including 13 heteromorphs. Only two of these ammonite taxa were previously recorded from the upper upper Albian at Clansayes, which demonstrates the value of this fauna with regard to taxonomy, palaeobiology and palaeobiogeography. Based on morphological and biometric analyses performed on an extensive material (104 specimens), we discriminate two species for the heteromorphic ammonite genus Mariella Nowak, 1916 within the Mortoniceras rostratum Zone. In addition, we investigate shell chirality patterns in Mariella from the late Albian of southern France. Upon comparison of the Clansayes material with older material from the immediately underlying upper Albian Mortoniceras fallax Zone at the neighbouring Salazac locality, we identify an increase in the proportion of sinistral specimens. This observed increase in the frequency of sinistral Mariella specimens may hypothetically be part of a global evolutionary pattern, considering that nearly all documented younger Cenomanian Mariella (and more generally Cenomanian turrilitids) are sinistral. [ABSTRACT FROM AUTHOR]

The study of development is critical for revealing the evolution of major vertebrate lineages. Coelacanths have one of the longest evolutionary histories among osteichthyans, but despite access to extant representatives, the onset of their weakly ossified endoskeleton is still poorly understood. Here we present the first palaeohistological and skeletochronological study of Miguashaia bureaui from the Upper Devonian of Canada, pivotal for exploring the palaeobiology and early evolution of osteogenesis in coelacanths. Cross sections of the caudal fin bones show that the cortex is made of layers of primary bone separated by lines of arrested growth, indicative of a cyclical growth. The medullary cavity displays remnants of calcified cartilage associated with bony trabeculae, characteristic of endochondral ossification. A skeletochronological analysis indicates that rapid growth during a short juvenile period was followed by slower growth in adulthood. Our new analysis highlights the life history and palaeoecology of Miguashaia bureaui and reveals that, despite differences in size and habitat, the poor endoskeletal ossification known in the extant Latimeria chalumnae can be traced back at least 375 million years ago. [ABSTRACT FROM AUTHOR]

Abstract Background Lemurs once rivalled the diversity of rest of the primate order despite thier confinement to the island of Madagascar. We test the adaptive radiation model of Malagasy lemur diversity using a novel combination of phylogenetic comparative methods and geometric methods for quantifying tooth shape. Results We apply macroevolutionary model fitting approaches and disparity through time analysis to dental topography metrics associated with dietary adaptation, an aspect of mammalian ecology which appears to be closely related to diversification in many clades. Metrics were also reconstructed at internal nodes of the lemur tree and these reconstructions were combined to generate dietary classification probabilities at internal nodes using discriminant function analysis. We used these reconstructions to calculate rates of transition toward folivory per million-year intervals. Finally, lower second molar shape was reconstructed at internal nodes by modelling the change in shape of 3D meshes using squared change parsimony along the branches of the lemur tree. Our analyses of dental topography metrics do not recover an early burst in rates of change or a pattern of early partitioning of subclade disparity. However, rates of change in adaptations for folivory were highest during the Oligocene, an interval of possible forest expansion on the island. Conclusions There was no clear phylogenetic signal of bursts of morphological evolution early in lemur history. Reconstruction of the molar morphologies corresponding to the ancestral nodes of the lemur tree suggest that this may have been driven by a shift toward defended plant resources, however. This suggests a response to the ecological opportunity offered by expanding forests, but not necessarily a classic adaptive radiation initiated by dispersal to Madagascar.

The Permo-Triassic mass extinction has been resolved into two closely spaced crises that both saw enormous extinction losses. However, food web modelling suggests they were not ecologically equivalent, only the second destabilised communities. [ABSTRACT FROM AUTHOR]

Valentine and Moores [Valentine JW, Moores EM (1970) Nature 228:657-659] hypothesized that plate tectonics regulates global biodiversity by changing the geographic arrangement of continental crust, but the data required to fully test the hypothesis were not available. Here, we use a global database of marine animal fossil occurrences and a paleogeographic reconstruction model to test the hypothesis that temporal patterns of continental fragmentation have impacted global Phanerozoic biodiversity. We find a positive correlation between global marine invertebrate genus richness and an independently derived quantitative index describing the fragmentation of continental crust during supercontinental coalescence-breakup cycles. The observed positive correlation between global biodiversity and continental fragmentation is not readily attributable to commonly cited vagaries of the fossil record, including changing quantities of marine rock or time-variable sampling effort. Because many different environmental and biotic factors may covary with changes in the geographic arrangement of continental crust, it is difficult to identify a specific causal mechanism. However, cross-correlation indicates that the state of continental fragmentation at a given time is positively correlated with the state of global biodiversity for tens of millions of years afterward. There is also evidence to suggest that continental fragmentation promotes increasing marine richness, but that coalescence alone has only a small negative or stabilizing effect. Together, these results suggest that continental fragmentation, particularly during the Mesozoic breakup of the supercontinent Pangaea, has exerted a first-order control on the long-term trajectory of Phanerozoic marine animal diversity.

Palaeobiological and archeological excavations at the site of Ewass Oldupa, found in the western Plio-Pleistocene rift basin of Oldupai Gorge (also Olduvai Gorge), Tanzania, revealed rich fossiliferous levels and the earliest remains of human activity at Oldupai Gorge, dated to 2 million years ago. This paper provides zooarchaeological taxonomic, taphonomic, and behavioral analyses, applying several methods to explore the setting in which the assemblage was formed. We identified agency behind bone surface modifications, such as cut, tooth and percussion marks, and determined the frequency of carnivore tooth marks as well as their distribution on both discrete specimens and across species. In addition, our work revealed co-occurrence of modifications to include butchering marks and carnivore tooth marks. Ravaging levels were estimated as percentage. The faunal accumulation from Ewass Oldupa contains two cut marked specimens, together with low degrees of percussion and carnivore tooth marks, moderate ravaging, and diagenetic changes suggestive of water flow. Thus, multiple lines of evidence indicate a palimpsest accumulation. Taxonomic diversity is high, with up to 22 taxa representing diverse habitats, ranging from open grassland to wooded bushlands, as well as moist mosaics during Bed I. Overall, this archaeo-faunal assemblage speaks to increased behavioral versatility among Oldowan hominins and interactions with the carnivore guild.

Summary: Archaeological and paleontological records offer tremendous yet often untapped potential for examining long-term biodiversity trends and the impact of climate change and human activity on ecosystems. Yet, zooarchaeological and fossil remains suffer various limitations, including that they are often highly fragmented and morphologically unidentifiable, preventing them from being optimally leveraged for addressing fundamental research questions in archaeology, paleontology, and conservation paleobiology. Here, we explore the potential of palaeoproteomics—the study of ancient proteins—to serve as a critical tool for creating richer, more informative datasets about biodiversity change that can be leveraged to generate more realistic, constructive, and effective conservation and restoration strategies into the future.

Summary: Sicily is a key region for understanding the agricultural transition in the Mediterranean because of its central position. Here, we present genomic and stable isotopic data for 19 prehistoric Sicilians covering the Mesolithic to Bronze Age periods (10,700–4,100 yBP). We find that Early Mesolithic hunter-gatherers (HGs) from Sicily are a highly drifted lineage of the Early Holocene western European HGs, whereas Late Mesolithic HGs carry ∼20% ancestry related to northern and (south) eastern European HGs, indicating substantial gene flow. Early Neolithic farmers are genetically most similar to farmers from the Balkans and Greece, with only ∼7% of ancestry from local Mesolithic HGs. The genetic discontinuities during the Mesolithic and Early Neolithic match the changes in material culture and diet. Three outlying individuals dated to ∼8,000 yBP; however, suggest that hunter-gatherers interacted with incoming farmers at Grotta dell’Uzzo, resulting in a mixed economy and diet for a brief interlude at the Mesolithic-Neolithic transition.

Summary: The Iron Age period occupies an important place in French history because the Gauls are regularly presented as the direct ancestors of the extant French population. We documented here the genomic diversity of Iron Age communities originating from six French regions. The 49 acquired genomes permitted us to highlight an absence of discontinuity between Bronze Age and Iron Age groups in France, lending support to a cultural transition linked to progressive local economic changes rather than to a massive influx of allochthone groups. Genomic analyses revealed strong genetic homogeneity among the regional groups associated with distinct archaeological cultures. This genomic homogenization appears to be linked to individuals’ mobility between regions and gene flow with neighbouring groups from England and Spain. Thus, the results globally support a common genomic legacy for the Iron Age population of modern-day France that could be linked to recurrent gene flow between culturally differentiated communities.

The combined use of the meiofauna (benthic foraminifers and ostracods) and geochemical data from three cores, recovered in distinct bathymetric sectors of the southern Adriatic shelf (Apulia, SE Italy), provides new insights on the relationships between shallow-marine environments, sediment fluxes and eutrophication trends over the last ∼7000 years. The stratigraphic distribution of meiofauna assemblages allows distinguishing a variety of facies associations, accumulated at proximal (Manfredonia Gulf), intermediate and distal shelf sectors. Spatial-temporal facies trajectories mainly reflect changes in fluxes of fine-grained, inorganic and organic particles at the seafloor. Major palaeoenvironmental shifts are paralleled by stratigraphic variations of Cr-Ni values, which represent effective provenance tracers for the Adriatic region. The correlation between the meiofauna content and trace-metal concentrations documents the link between the type of substrate (organic matter-OM and grain-size) and sediment provenance along the bathymetric profile and through time. After 6000 yr B.P., the gulf, partly sheltered by the Gargano Promontory, experienced the first increase in OM fluxes (transition from open-bay to prodelta). This turnaround occurred under the progressive change in sediment supply from southern Apennine source-rocks, poor in trace metals, to a mixed contribution including northern sources, enriched in chromium and nickel (mafic/ultramafic rocks of the Po River catchment) via the SSE-directed longshore drift. Alongside, prodelta clays in lateral transition to mud-belt deposits accumulated in the adjacent open shelf under the steady influence of the longshore drift. Finally, since ∼2000 yr B.P. a pervasive subaqueous delta system developed across the study area in response to increased sediment load by Po + Apennine rivers, likely induced by enhanced anthropic pressure during Roman times. This study highlights the key role played by the sediment routing system, coastal morphology and human land-use on shelf facies patterns and eutrophication trends, even in areas hundreds of km away from the main fluvial mouths. • Shallow-marine facies, meiofauna and sediment provenance are closely related. • Beyond water depth, meiofauna reflect organic content and grain-size sea bottom. • Longshore drift coupled with coastal morphology drives shelf facies patterns. • Human land-use can fuel environmental changes on shelves far from major deltas. [ABSTRACT FROM AUTHOR]

Allometric scaling describes the relationship of trait size to body size within and among taxa. The slope of the population‐level regression of trait size against body size (i.e. static allometry) is typically invariant among closely related populations and species. Such invariance is commonly interpreted to reflect a combination of developmental and selective constraints that delimit a phenotypic space into which evolution could proceed most easily. Thus, understanding how allometric relationships do eventually evolve is important to understanding phenotypic diversification. In a lineage of fossil Threespine Stickleback (Gasterosteus doryssus), we investigated the evolvability of static allometric slopes for nine traits (five armour and four non‐armour) that evolved significant trait differences across 10 samples over 8500 years. The armour traits showed weak static allometric relationships and a mismatch between those slopes and observed evolution. This suggests that observed evolution in these traits was not constrained by relationships with body size, perhaps because prior, repeated adaptation to freshwater habitats by Threespine Stickleback had generated strong selection to break constraint. In contrast, for non‐armour traits, we found stronger allometric relationships. Those allometric slopes did evolve on short time scales. However, those changes were small and fluctuating and the slopes remained strong predictors of the evolutionary trajectory of trait means over time (i.e. evolutionary allometry), supporting the hypothesis of allometry as constraint. [ABSTRACT FROM AUTHOR]

The latest Cretaceous kogaionid multituberculates from Transylvania (western Romania) were part of an endemic European clade of mammals that underwent an insular radiation at the end of the Cretaceous and then survived the end-Cretaceous mass extinction that extinguished many groups of contemporary therians. Transylvanian kogaionids lived on what was an island during the latest Cretaceous-"Hațeg Island"-and their fossils are found in the uppermost Campanian to upper Maastrichtian deposits of the Hațeg, Rusca Montană, and southwestern Transylvanian basins. This fossil record has improved dramatically over the past several decades, in part resulting from our decade-long joint Romanian-American-Scottish fieldwork, and comprises one of the most impressive and complete archives of Mesozoic mammals, including not only jaws and teeth but several incomplete skulls and partial skeletons. We here review the fossil record of kogaionids from Transylvania. We report four new occurrences from the Hațeg Basin, update information on previously described ones, and use our database to reassess the chronostratigraphical and geographical distribution of kogaionids and their evolutionary patterns. Although it was previously suggested that large and small kogaionids had largely mutually exclusive spatial distributions, we recognize the cooccurrence of small and large taxa in various units, suggesting a sympatric distribution across their entire chronostratigraphic range. We also identify a novel pattern: small kogaionids appear somewhat earlier than their larger relatives in all well-sampled sedimentary successions, suggesting that kogaionid colonizations of Hațeg Island and component regions took place at small body size and that body size increased only later through local evolution. We find correlations between body size, preservation style, and sedimentary context, which give insight into kogaionid paleobiology and diversity. Larger kogaionids are represented more often by partial skulls and occasionally skeletons compared with small kogaionids, which are usually represented only by isolated teeth, regardless of provenance. Larger kogaionids currently have a higher recognized local taxic diversity than their smaller relatives. We hypothesize that this may be in part a consequence of preservational bias related to body size, as more complete specimens may be more easily diagnosed as distinct taxa than those that are represented by more fragmentary and/or incomplete fossils. If true, the taxic diversity of smaller kogaionids may currently be underestimated. Finally, we identify correspondence between sedimentary facies and preservation style. Red-colored fine-grained rocks, suggestive of well-drained, oxidized floodplain paleoenvironments, yield more complete specimens than drab, greenish or grayish sediments deposited in more poorly drained parts of the floodplain. This pattern may suggest habitat preferences for betterdrained floodplain environments and a semifossorial lifestyle for some taxa. As the kogaionid fossil record improves, we can further test the hypotheses and patterns outlined above. The pace of new kogaionid discoveries by our team and others indicates that a more complete picture of kogaionid distribution, paleobiology, and evolution will emerge in the coming years, contributing to a more profound understanding of this peculiar group of island-dwelling Mesozoic mammals. [ABSTRACT FROM AUTHOR]

In this paper, we study the postcranial morphology (humerus, ulna, innominate, femur, tibia, astragalus, navicular, and metatarsal III) of Neoepiblema, a giant Late Miocene South American rodent, searching for evidence about its paleobiology based on unpublished specimens from Solimões Formation (Upper Miocene, Brazil). The study includes a morphofunctional analysis of the postcranial bones and a comparison with extant and extinct rodents, especially Phoberomys. The morphofunctional analysis of the postcranial bones suggests that Neoepiblema (as well as Phoberomys) would have a crouched forelimb that was not fully extended, with powerful pectoral and triceps musculature, and able to produce movements of pronation/supination and possibly with a hand able to grasp. The combination of characters of the innominate bone, femur, and tibia indicates a predominance of parasagittal movements and a thigh with powerful musculature used during propulsion. In sum, the analyzed postcranial features are consistent with the limb morphology of ambulatory rodents, but with faculty to dig or swim. The sedimentary evidence of the localities in which fossils of neoepiblemids have been found suggests that these rodents lived in wet and water-related environments (near swamps, lakes, and/or rivers). [ABSTRACT FROM AUTHOR]

A recent article argued that signals from conventional Raman spectroscopy of organic materials are overwhelmed by edge filter and fluorescence artefacts. The article targeted a subset of Raman spectroscopic investigations of fossil and modern organisms and has implications for the utility of conventional Raman spectroscopy in comparative tissue analytics. The inferences were based on circular reasoning centered around the unconventional analysis of spectra from just two samples, one modern, and one fossil. We validated the disputed signals with in situ Fourier‐Transform Infrared (FT‐IR) Spectroscopy and through replication with different lasers, filters, and operators in independent laboratories. Our Raman system employs a holographic notch filter which is not affected by edge filter or other artefacts. Multiple lines of evidence confirm that conventional Raman spectra of fossils contain biologically and geologically meaningful information. Statistical analyses of large Raman and FT‐IR spectral data sets reveal patterns in fossil composition and yield valuable insights into the history of life. [ABSTRACT FROM AUTHOR]