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New zircon and apatite fission-track analyses and aluminium in hornblende thermobarometric data are presented from Cretaceous and Triassic plutons situated along an east-west transect across the Central Cordillera, the Cauca Romeral Fault System and the eastern slopes of the Western Cordillera in the Colombian Andes. The results are combined with published apatite (U–Th)/He ages revealing three episodes of increased cooling during the Late Cretaceous (~110 - 90 Ma), the Eocene (~55-40 Ma) and the Miocene (23-20 Ma). The latter two phases correlate well with the Pre-Andean and Proto-Andean periods previously identified on the basis of stratigraphic unconformities and thermochronology studies in the region. A fourth event, at ~10 Ma, is tentatively recognized in modelled data sets, but not by absolute age values. The thermochronology data imply a total of uplift-driven exhumation (i.e., crustal unroofing) in the study area of up to 10 kms since ~50 Ma at rates of ~0.1 km/Myr and 4 kms since ~23 Ma initially at 0.2 km/Myr. From 10 Ma the rates changed to 0.3–0.33 km/Myr with estimated geothermal gradients of 25–30 °C/km. Thermobarometry data suggest that the crystallization of the Cretaceous intrusions, now at the surface, took place at depths of 8–13 km confirming the amount of exhumation revealed by low-temperature thermochronology. • New thermochronology ages for Northern Andes combining apatite and zircon FT. • Thermochronology/thermobarometry dataset in the Northern Andes of Colombia. • Vertical profile shows pulses of uplift exhumation at ~110–90, 45–50 and 23 Ma. • Orogenic events involving erosional cooling and crustal unroofing from ~8 to 13 km. • Orogenic events are coeval with pulses in the Caribbean, Southern-Central Andes. [ABSTRACT FROM AUTHOR]

Context. Quantifying the number density and physical characteristics of extragalactic polarized sources is important for the successful planning of future studies based on Faraday rotation measure (RM) grids of polarized sources to probe foreground Galactic and intergalactic magnetic fields. However, it is proving very hard to detect polarized signal from the population of very faint (sub-mJy) polarized sources at low radio frequencies, and their properties are mostly unknown. LOFAR can play an important role in such studies thanks to its sensitivity and angular resolution, combined with the precision on the inferred RM values that can be achieved through low-frequency broad-band polarimetry. Aims. The aim of this study is to probe the sub-mJy polarized source population with LOFAR. In this first paper, we present the method used to stack LOFAR polarization datasets, the resulting catalog of polarized sources, and the derived polarized source counts. Methods. The European Large Area ISO Survey-North 1 (ELAIS-N1) field, one of the deepest of the LOFAR Two-Metre Sky Survey (LoTSS) Deep Fields so far, was selected for a polarimetric study at 114.9–177.4 MHz. A total area of 25 deg2 was imaged at 6″-resolution in the Stokes Q and U parameters. Alignment of polarization angles was done both in frequency and in Faraday space before stacking datasets from 19 eight-hour-long epochs taken in two different LOFAR observing cycles. A search for polarized sources was carried out in the final, stacked dataset, and the properties of the detected sources were examined. The depolarization level of sources known to be polarized at 1.4 GHz was quantified. Results. A one-sigma noise level, σQU, of 19 µJy beam−1 was reached in the central part of the field after stacking. Twenty-five polarized sources were detected above 8σQU, five of which had not been detected in polarization at any other radio frequencies before. Seven additional polarized components were found by lowering the threshold to 6σQU at positions corresponding to sources known to be polarized at 1.4 GHz. In two radio galaxies, polarization was detected from both radio lobes, so the final number of associated radio continuum sources is 31. The detected sources are weakly polarized, with a median degree of polarization of 1.75% for the sample of sources detected in polarized emission. For the 10 polarized sources previously identified in a pilot LOFAR study of the ELAIS-N1 field at 20″-resolution, the RM values are consistent but the degrees of polarization are higher in the 6″-resolution data. The sources previously detected in polarization at 1.4 GHz are significantly depolarized at 150 MHz. The catalog is used to derive the polarized source counts at 150 MHz. Conclusions. This is the deepest and highest-resolution polarization study at 150 MHz to date. A full characterization of the sources and an analysis of the catalog will be presented in Paper II. [ABSTRACT FROM AUTHOR]

This article is based on a research that explored the relations between the Personal Social Network of people who have been displaced because of political violence and their Quality of Life in Health. These relations can be modified by the time of being in the new place of refuge - less than three months and more of one year. The hypothesis looked for if to greater density of the Personal Social Network of displaced people the Quality of Life in Health was low, which together with other social factors would reduce their Sense of community and capacity of adaptation. The data were collected in Pinar del Río (periphery of Barranquilla - Colombia), with 19 people selected in snow ball sample (8 men, 11 women, between 27 and 61 years). They responded to a questionnaire on Quality of Life in Health (Sf-36) (McHorney et al., 1994), and the Arizona Social Support Interview Schedule (ASSIS) of Barrera (1980, cited by Araya and Maya, 2005). The results do not show significant correlation between the time of refuge with the study variables, but it was observed that the Quality of Life, in its dimension of physic health, is smaller in displaced people with more of one year in the new place of refuge, with respect to displaced people who have less than three months in the new place of refuge.

Plant trait information is essential for understanding plant evolution, vegetation dynamics, and vegetation responses to disturbance and management. Furthermore, in Mediterranean ecosystems, changes in fire regime may be more relevant than direct changes in climatic conditions, making the knowledge of fire-related traits especially important. Thus the purpose of this data set was to compile the most updated and comprehensive information on fire-related traits for vascular plant species of the Mediterranean Basin, that is, traits related to plant persistence and regeneration after fire. Data were collected from an extensive literature review and from field and experimental observations. The data source is documented for each value. Since life history traits may vary spatially or with environmental conditions, we did not aggregate them by species; i.e., traits and species are repeated in different records if they were observed by different researchers and/or in different locations. Life history traits included in the data set are: life form, resprouting ability (after fire, after clipping, or after other disturbances that remove all the aboveground biomass), resprouting bud source, heat-stimulated germination, other germination cues, seed bank location and longevity, post-fire seedling emergence and survival, maturity age of resprouts and saplings, and seed mass. Several traits are unknown for many species; consequently, the data set reflects the state of the knowledge on the topic. However, since the ability to resprout is a trait of paramount relevance in fire-prone environments, it was considered a core trait in the data set, and thus species whose resprouting capacity was unknown were not included. Life form is also provided for all taxa. The structure of the database allows different levels of information (and accuracy) for each entry, and thus some traits may include different types of data (quantitative, semi-quantitative, or categorical) from different sources. The data set is structured in 8263 records and II columns, obtained from 301 published and unpublished sources of information. It includes 952 taxa determined at specific or infraspecific level, which comprise 859 species, 384 genera, and 79 families. Although this is the most comprehensive data set of fire-relevant plant traits for Mediterranean species, there is still a considerable need for observations and experiments, especially in little-studied Mediterranean areas, such as northern Africa. [ABSTRACT FROM AUTHOR]

Abstract: Conversion of limonene in its derivatives has been an interesting process due to its high value for cosmetic, pharmaceutical and food industries. The use of molecular sieves based on transition metals in heterogeneous catalysis, and specifically in oxidative processes, has shown good results. Oxidation of limonene has been studied using zinc based catalyst, hydrogen peroxide as oxidative agent and t-butanol or tetrahydrofurane as solvents at different reaction times and temperatures. 1,2-Epoxi-limonene, carvone, carveol and limonene-1,2-diol were obtained as main products. [Copyright &y& Elsevier]

Kulcsszavak (angolul, ha nem azonos az elozovel) [ABSTRACT FROM AUTHOR]

• A new compact 10 cm height and diameter tension disc infiltrometer is presented. • The water level is monitored by a smartphone camera. • The device was satisfactory compared in laboratory with V and PT methods. • Accurate estimate of hydraulic conductivity and sorptivity were obtained. • The method is an accurate, low-cost, portable and easy to use in field. The tension disc infiltrometer has become a widespread method to measure soil hydraulic properties under unsaturated conditions. Although several automated devices have been developed, most designs include an elongated water reservoir and a pressure transducer with electronic components to register the water level, increasing its cost and limiting its application. The objective of this work is to present a new tension disc infiltrometer with a compact design of 10 cm diameter and height, where the water level is monitored by a smartphone camera. The infiltration curve is determined from the automated analysis of the images recorded by the smartphone without additional electronic components. The device was first validated in the laboratory by comparing visual measurements (V), the camera imagery (Ca) and a pressure transducer (PT). Next, it was tested on field infiltration experiments. Robust fits (R2 = 0.99) were found between the water level measured with Ca in the laboratory and those obtained with V and PT procedures. The Ca method is accurate, robust and independent of the relative camera position. Good fits were also observed between Ca water level and those obtained with PT in the field experiments. Similar hydraulic conductivity and sorptivity values were obtained with both sensors using the numerical solution of the Haverkamp (NSH) equation. The compact infiltrometer, in conjunction with the smartphone camera, is an accurate, accessible, portable and easy-to-use field-based device for soil hydraulic characterisation. [ABSTRACT FROM AUTHOR]

Diffusion models (DMs) have disrupted the image super-resolution (SR) field and further closed the gap between image quality and human perceptual preferences. They are easy to train and can produce very high-quality samples that exceed the realism of those produced by previous generative methods. Despite their promising results, they also come with new challenges that need further research: high computational demands, comparability, lack of explainability, color shifts, and more. Unfortunately, entry into this field is overwhelming because of the abundance of publications. To address this, we provide a unified recount of the theoretical foundations underlying DMs applied to image SR and offer a detailed analysis that underscores the unique characteristics and methodologies within this domain, distinct from broader existing reviews in the field. This article articulates a cohesive understanding of DM principles and explores current research avenues, including alternative input domains, conditioning techniques, guidance mechanisms, corruption spaces, and zero-shot learning approaches. By offering a detailed examination of the evolution and current trends in image SR through the lens of DMs, this article sheds light on the existing challenges and charts potential future directions, aiming to inspire further innovation in this rapidly advancing area.

Dengue fever remains as a public health challenge in Colombia, standing as the most prevalent infectious disease in the country. The cyclic nature of dengue epidemics, occurring approximately every 3 years, is intricately linked to meteorological events like El Niño Southern Oscillation (ENSO). Therefore, the Colombian system faces challenges in genomic surveillance. This study aimed to evaluate local dengue virus (DENV) transmission and genetic diversity in four Colombian departments with heterogeneous incidence patterns (department is first-level territorial units in Colombia). For this study, we processed 266 serum samples to identify DENV. Subsequently, we obtained 118 genome sequences by sequencing DENV genomes from serum samples of 134 patients infected with DENV-1 and DENV-2 serotypes. The predominant serotype was DENV-2 (108/143), with the Asian-American (AA) genotype (91/118) being the most prevalent one. Phylogenetic analysis revealed concurrent circulation of two lineages of both DENV-2 AA and DENV-1 V, suggesting ongoing genetic exchange with sequences from Venezuela and Cuba. The continuous migration of Venezuelan citizens into Colombia can contribute to this exchange, emphasizing the need for strengthened prevention measures in border areas. Notably, the time to most recent common ancestor analysis identified cryptic transmission of DENV-2 AA since approximately 2015, leading to the recent epidemic. This challenges the notion that major outbreaks are solely triggered by recent virus introductions, emphasizing the importance of active genomic surveillance. The study also highlighted the contrasting selection pressures on DENV-1 V and DENV-2 AA, with the latter experiencing positive selection, possibly influencing its transmissibility. The presence of a cosmopolitan genotype in Colombia, previously reported in Brazil and Peru, raises concerns about transmission routes, emphasizing the necessity for thorough DENV evolution studies. Despite limitations, the study underscores genomic epidemiology's crucial role in early detection and comprehension of DENV genotypes, recommending the use of advanced sequencing techniques as an early warning system to help prevent and control dengue outbreaks in Colombia and worldwide., Competing Interests: None declared., (© The Author(s) 2024. Published by Oxford University Press.)

Background and Aims: Soil endemics have long fascinated botanists due to the insights they can provide about plant ecology and evolution. Often, these species have unique foliar nutrient composition patterns that reflect potential physiological adaptations to these harsh soil types. However, understanding global nutritional patterns to unique soil types can be complicated by the influence of recent and ancient evolutionary events. Our goal was to understand whether plant specialization to unique soils is a stronger determinant of plant nutrient composition than climate or evolutionary constraints., Methods: We worked on gypsum soils. We analyzed whole-plant nutrient composition (leaves, stems, coarse roots and fine roots) of 36 native species of gypsophilous lineages from the Chihuahuan Desert (North America) and the Iberian Peninsula (Europe) regions, including widely distributed gypsum endemics, as specialists, and narrowly distributed endemics and non-endemics, as non-specialists. We evaluated the impact of evolutionary events and soil composition on the whole-plant composition, comparing the three categories of gypsum plants., Key Results: Our findings reveal nutritional convergence of widely distributed gypsum endemics. These taxa displayed higher foliar Sulfur and higher whole-plant Magnesium than their non-endemic relatives, irrespective of geographic location or phylogenetic history. Sulfur and Magnesium concentrations were mainly explained by non-phylogenetic variation among species related to gypsum specialization. Other nutrient concentrations were determined by more ancient evolutionary events. For example, Caryophyllales usually displayed high foliar Calcium, whereas Poaceae did not. In contrast, plant concentrations of Phosphorus was mainly explained by species-specific physiology not related to gypsum specialization or evolutionary constraints., Conclusions: Plant specialization to a unique soil may strongly influence plant nutritional strategies, as we described for gypsophilous lineages. Taking a whole-plant perspective (all organs) within a phylogenetic framework has enabled us to gain a better understanding of plant adaptation to unique soils when studying taxa from distinct regions., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Using Oxford Nanopore technologies and phylogenetic analyses, we sequenced and identified the cosmopolitan genotype of dengue virus serotype 2 isolated from 2 patients in the city of Villavicencio, Meta department, Colombia. This identification suggests the emergence of this genotype in the country, which warrants further surveillance to identify its epidemic potential.

Introduction: Currently the patient is defined as an older adult (OA) when the age is at least 60 years. Given the long life expectancy, it is interesting to evaluate whether all OAs with acute myocardial infarction (AMI) are equal. The objectives were to know the prevalence of OA in AMI and within them, that of those ≥75 years of age and to analyze characteristics, reperfusion treatments and in-hospital mortality according to whether they are < or ≥ 75 years of age., Methods: OA patients admitted to the National Registry of Infarction with ST segment elevation (ARGENIAM-ST) were analyzed. They were divided into group 1: 60-74 years old and group 2: ≥ 75 years old and compared with each other., Results: 3626 AM, 75.9% from Group 1, the rest from Group 2. In group 2 there were more women, hypertensive and with a history of coronary arteries. There was a similar percentage of diabetes and dyslipidemia, but fewer of smokers. In Group 2, less reperfusion treatment was used (although more primary angioplasty), with similar door-to-balloon time. Patients in Group 2 received fewer medications of proven efficacy and in the hospital course, they had more bleeding (although not major), more heart failure and more mortality: 18.3% vs. 9.4%, p<0.001. Age ≥75 years was an independent predictor of mortality., Conclusions: one in four patients with AMI is over 75 years old; they receive less reperfusion, have more heart failure, bleeding and twice the mortality rate than patients between 60 and 74 years.

Calcium biomineralisation is widely documented in plants. However, crystallisation of Ca-sulphate-containing minerals is closely related to water content, and sample processing, such as drying, alters the water balance of plant tissues. We hypothesised that common sample processing practices may favour the formation of crystals, leading to spurious crystallisation not observed in unaltered plant tissues. We selected three species (Ononis tridentata, Helianthemum squamatum and Gypsophila struthium) with reported gypsum biomineralisation. We used x-ray diffractometry on fresh intact or sliced leaves, and on the same leaves processed by subsequent drying, to address whether sample processing alters crystal formation. Ca-sulphate crystals were detected in dry samples of all species but not in fresh intact samples. Ca-sulphate crystallisation occurred in some cut fresh samples, although the accumulation greatly increased after drying. In addition, G. struthium exhibited Ca-oxalate crystals in both fresh and dry treatments, with a tendency for greater accumulation in dry treatments. Our results demonstrate that the Ca-sulphate crystals observed by x-ray diffractometry in these species are artefacts caused by common sample processing practices, such as excessive drying and slicing samples. We encourage future studies on the biomineral potential of plants to avoid the use of procedures that alter the water balance of tissues., (© 2023 Scandinavian Plant Physiology Society.)

With the advent of Deep Learning (DL), Super-Resolution (SR) has also become a thriving research area. However, despite promising results, the field still faces challenges that require further research, e.g., allowing flexible upsampling, more effective loss functions, and better evaluation metrics. We review the domain of SR in light of recent advances and examine state-of-the-art models such as diffusion (DDPM) and transformer-based SR models. We critically discuss contemporary strategies used in SR and identify promising yet unexplored research directions. We complement previous surveys by incorporating the latest developments in the field, such as uncertainty-driven losses, wavelet networks, neural architecture search, novel normalization methods, and the latest evaluation techniques. We also include several visualizations for the models and methods throughout each chapter to facilitate a global understanding of the trends in the field. This review ultimately aims at helping researchers to push the boundaries of DL applied to SR.

Gypsum soils are amongst the most widespread extreme substrates of the world, occurring in 112 countries. This type of hypercalcic substrate has a suite of extreme physical and chemical properties that make it stressful for plant establishment and growth. Extreme chemical properties include low plant-available nitrogen and phosphorus and high plant-available sulphur and calcium, which impose strong nutritional imbalances on plants. In spite of these edaphic barriers, gypsum soils harbour rich endemic floras that have evolved independently on five continents, with highly specialized species. Plants that only grow on gypsum are considered soil specialists, and they have a foliar elemental composition similar to the elemental availability of gypsum soils, with high calcium, sulphur and magnesium accumulation. However, the physiological and ecological role of the unique foliar elemental composition of gypsum specialists remains poorly understood, and it is unknown whether it provides an ecological advantage over other generalist species on gypsum soils. This article reviews available literature on the impact of gypsum soil features on plant life and the mechanisms underlying plant adaptation to gypsum environments. We conclude with a hypothesis on the potential role of the nutritional strategy underlying plant specialization to gypsum soils: Gypsum specialists primarily use SO 4 2- as a counter anion to tolerate high Ca 2+ concentrations in cells and avoid phosphorus depletion, which is one of the most limiting nutrients in gypsum soils., Competing Interests: None declared., (© The Author(s) 2023. Published by Oxford University Press on behalf of the Annals of Botany Company.)

Serine/threonine protein phosphatase 2 (PP2A) forms heterotrimeric holoenzymes, where a scaffold subunit bridges the PP2A catalytic subunit to a B regulatory subunit, e.g., B55α. The PP2A/B55α holoenzyme plays key roles in signaling and cell-cycle control targeting multiple substrates. Here, we describe semiquantitative approaches to determine PP2A/B55α substrate specificity. Parts I and II detail approaches to assess PP2A/B55α-mediated dephosphorylation of immobilized substrate peptide variants. Parts III and IV detail methods to assess PP2A/B55α-substrate-binding specificity. These approaches are adaptable to other serine/threonine phosphatases. For complete details on the use and execution of this protocol, please refer to Fowle et al.. 1 ., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

Accretion disks around compact objects are expected to enter an unstable phase at high luminosity 1 . One instability may occur when the radiation pressure generated by accretion modifies the disk viscosity, resulting in the cyclic depletion and refilling of the inner disk on short timescales 2 . Such a scenario, however, has only been quantitatively verified for a single stellar-mass black hole 3-5 . Although there are hints of these cycles in a few isolated cases 6-10 , their apparent absence in the variable emission of most bright accreting neutron stars and black holes has been a continuing puzzle 11 . Here we report the presence of the same multiwavelength instability around an accreting neutron star. Moreover, we show that the variability across the electromagnetic spectrum-from radio to X-ray-of both black holes and neutron stars at high accretion rates can be explained consistently if the accretion disks are unstable, producing relativistic ejections during transitions that deplete or refill the inner disk. Such a new association allows us to identify the main physical components responsible for the fast multiwavelength variability of highly accreting compact objects., (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)

The analysis of plant elemental composition and the underlying factors affecting its variation are a current hot topic in ecology. Ecological adaptation to atypical soils may shift plant elemental composition. However, no previous studies have evaluated its relevance against other factors such as phylogeny, climate or individual soil conditions. We evaluated the effect of the phylogeny, environment (climate, soil), and affinity to gypsum soils on the elemental composition of 83 taxa typical of Iberian gypsum ecosystems. We used a new statistical procedure (multiple phylogenetic variance decomposition, MPVD) to decompose total explained variance by different factors across all nodes in the phylogenetic tree of target species (covering 120 million years of Angiosperm evolution). Our results highlight the relevance of phylogeny on the elemental composition of plants both at early (with the development of key preadaptive traits) and recent divergence times (diversification of the Iberian gypsum flora concurrent with Iberian gypsum deposit accumulation). Despite the predominant phylogenetic effect, plant adaptation to gypsum soils had a strong impact on the elemental composition of plants, particularly on sulphur concentrations, while climate and soil effects were smaller. Accordingly, we detected a convergent evolution of gypsum specialists from different lineages on increased sulphur and magnesium foliar concentrations., (© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)

Extreme soils often have mineral nutrient imbalances compared to plant nutritional requirements and co-occur in open areas where grazers thrive. Thus, plants must respond to both constraints, which can affect nutrient concentrations in all plant organs. Gypsum soil provides an excellent model system to study adaptations to extreme soils under current grazing practices as it harbours two groups of plant species that differ in their tolerance to gypsum soils and foliar composition. However, nutrient concentrations in organs other than leaves, and their individual responses to simulated herbivory, are still unknown in gypsum plants. We studied plant biomass, root mass ratio and nutrient partitioning among different organs (leaves, stems, coarse roots, fine roots) in five gypsum endemics and five generalists cultivated in gypsum and calcareous soils and subjected to different levels of simulated browsing. Gypsum endemics tended to have higher elemental concentration in leaves, stems and coarse roots than generalist species in both soil types, whereas both groups tended to show similar high concentrations in fine roots. This behaviour was especially clear with sulphur (S), which is found in excess in gypsum soils, and which endemics accumulated in leaves as sulphate (>50% of S). Moreover, plants subjected to clipping, regardless of their affinity to gypsum, were unable to compensate for biomass losses and showed similar elemental composition to unclipped plants. The accumulation of excess mineral nutrients by endemic species in aboveground organs may be a constitutive nutritional strategy in extreme soils and is potentially playing an anti-herbivore role in grazed gypsum outcrops., (© 2022 The Authors. Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.)

All disc-accreting astrophysical objects produce powerful disc winds. In compact binaries containing neutron stars or black holes, accretion often takes place during violent outbursts. The main disc wind signatures during these eruptions are blue-shifted X-ray absorption lines, which are preferentially seen in disc-dominated 'soft states' 1,2 . By contrast, optical wind-formed lines have recently been detected in 'hard states', when a hot corona dominates the luminosity 3 . The relationship between these signatures is unknown, and no erupting system has as yet revealed wind-formed lines between the X-ray and optical bands, despite the many strong resonance transitions in this ultraviolet (UV) region 4 . Here we report that the transient neutron star binary Swift J1858.6-0814 exhibits wind-formed, blue-shifted absorption lines associated with C IV, N V and He II in time-resolved UV spectroscopy during a luminous hard state, which we interpret as a warm, moderately ionized outflow component in this state. Simultaneously observed optical lines also display transient blue-shifted absorption. Decomposing the UV data into constant and variable components, the blue-shifted absorption is associated with the former. This implies that the outflow is not associated with the luminous flares in the data. The joint presence of UV and optical wind features reveals a multi-phase and/or spatially stratified evaporative outflow from the outer disc 5 . This type of persistent mass loss across all accretion states has been predicted by radiation-hydrodynamic simulations 6 and helps to explain the shorter-than-expected duration of outbursts 7 ., (© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)

Salt marshes are unique habitats between sea or saline lakes and land that need to be conserved from the effects of global change. Understanding the variation in functional structure of plant community along environmental gradients is critical to predict the response of plant communities to ongoing environmental changes. We evaluated the changes in the functional structure of halophytic communities along soil gradients including salinity, in Iranian salt marshes; Lake Urmia, Lake Meyghan, Musa estuary, and Nayband Bay (Iran). We established 48 plots from 16 sites in four salt marshes and sampled 10 leaves per species to measure leaf functional traits. Five soil samples were sampled from each plot and 30 variables were analyzed. We examined the changes in the functional structure of plant communities (i.e., functional diversity [FD] and community weighted mean [CWM]) along local soil gradients using linear mixed effect models. Our results showed that FD and CWM of leaf thickness tended to increase with salinity, while those indices related to leaf shape decreased following soil potassium content. Our results suggest that the variations in functional structure of plant communities along local soil gradients reveal the effect of different ecological processes (e.g., niche differentiation related to the habitat heterogeneity) that drive the assembly of halophytic plant communities in SW Asian salt marshes., Competing Interests: The authors have no conflict of interests to declare., (© 2022 The Authors. Plant‐Environment Interactions published by New Phytologist Foundation and John Wiley & Sons Ltd.)

Background and Aims: Gypsum drylands are widespread worldwide. In these arid ecosystems, the ability of different species to access different water sources during drought is a key determining factor of the composition of plant communities. Gypsum crystallization water could be a relevant source of water for shallow-rooted plants, but the segregation in the use of this source of water among plants remains unexplored. We analysed the principal water sources used by 20 species living in a gypsum hilltop, the effect of rooting depth and gypsum affinity, and the interaction of the plants with the soil beneath them., Methods: We characterized the water stable isotope composition, δ 2H and δ 18O, of plant xylem water and related it to the free and gypsum crystallization water extracted from different depths throughout the soil profile and the groundwater, in both spring and summer. Bayesian isotope mixing models were used to estimate the contribution of water sources to plant xylem sap., Key Results: In spring, all species used free water from the top soil as the main source. In summer, there was segregation in water sources used by different species depending on their rooting depth, but not on their gypsum affinity. Gypsum crystallization water was the main source for most shallow-rooted species, whereas free water from 50 to 100 cm depth was the main source for deep-rooted species. We detected plant-soil interactions in spring, and indirect evidence of possible hydraulic lift by deep-rooted species in summer., Conclusions: Plants coexisting in gypsum communities segregate their hydrological niches according to their rooting depth. Crystallization water of gypsum represents an unaccounted for, vital source for most of the shallow-rooted species growing on gypsum drylands. Thus, crystallization water helps shallow-rooted species to endure arid conditions, which eventually accounts for the maintenance of high biodiversity in these specialized ecosystems., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Annals of Botany Company.)

Herbivory is one of the most globally distributed disturbances affecting carbon (C)-cycling in trees, yet our understanding of how it alters tree C-allocation to different functions such as storage, growth or rhizodeposition is still limited. Prioritized C-allocation to storage replenishment vs growth could explain the fast recovery of C-storage pools frequently observed in growth-reduced defoliated trees. We performed continuous 13C-labeling coupled to clipping to quantify the effects of simulated browsing on the growth, leaf morphology and relative allocation of stored vs recently assimilated C to the growth (bulk biomass) and non-structural carbohydrate (NSC) stores (soluble sugars and starch) of the different organs of two tree species: diffuse-porous (Betula pubescens Ehrh.) and ring-porous (Quercus petraea [Matt.] Liebl.). Carbon-transfers from plants to bulk and rhizosphere soil were also evaluated. Clipped birch and oak trees shifted their C-allocation patterns above-ground as a means to recover from defoliation. However, such increased allocation to current-year stems and leaves did not entail reductions in the allocation to the rhizosphere, which remained unchanged between clipped and control trees of both species. Betula pubescens and Q. petraea showed differences in their vulnerability and recovery strategies to clipping, the ring-porous species being less affected in terms of growth and architecture by clipping than the diffuse-porous. These contrasting patterns could be partly explained by differences in their C cycling after clipping. Defoliated oaks showed a faster recovery of their canopy biomass, which was supported by increased allocation of new C, but associated with large decreases in their fine root biomass. Following clipping, both species recovered NSC pools to a larger extent than growth, but the allocation of 13C-labeled photo-assimilates into storage compounds was not increased as compared with controls. Despite their different response to clipping, our results indicate no preventative allocation into storage occurred during the first year after clipping in either of the species., (© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

The accepted role of the protein Kv2.1 in arterial smooth muscle cells is to form K + channels in the sarcolemma. Opening of Kv2.1 channels causes membrane hyperpolarization, which decreases the activity of L-type Ca V 1.2 channels, lowering intracellular Ca 2+ ([Ca 2+ ] i ) and causing smooth muscle relaxation. A limitation of this model is that it is based exclusively on data from male arterial myocytes. Here, we used a combination of electrophysiology as well as imaging approaches to investigate the role of Kv2.1 channels in male and female arterial myocytes. We confirmed that Kv2.1 plays a canonical conductive role but found it also has a structural role in arterial myocytes to enhance clustering of Ca V 1.2 channels. Less than 1% of Kv2.1 channels are conductive and induce membrane hyperpolarization. Paradoxically, by enhancing the structural clustering and probability of Ca V 1.2-Ca V 1.2 interactions within these clusters, Kv2.1 increases Ca 2+ influx. These functional impacts of Kv2.1 depend on its level of expression, which varies with sex. In female myocytes, where expression of Kv2.1 protein is higher than in male myocytes, Kv2.1 has conductive and structural roles. Female myocytes have larger Ca V 1.2 clusters, larger [Ca 2+ ] i , and larger myogenic tone than male myocytes. In contrast, in male myocytes, Kv2.1 channels regulate membrane potential but not Ca V 1.2 channel clustering. We propose a model in which Kv2.1 function varies with sex: in males, Kv2.1 channels control membrane potential but, in female myocytes, Kv2.1 plays dual electrical and Ca V 1.2 clustering roles. This contributes to sex-specific regulation of excitability, [Ca 2+ ] i , and myogenic tone in arterial myocytes., Competing Interests: Competing interest statement: L.F.S. and M.T.N. are both affiliated with the University of Vermont., (Copyright © 2020 the Author(s). Published by PNAS.)

Objective: Treatment options for HER-2-positive metastatic breast cancer (mBC) patients have expanded markedly since trastuzumab approval in 1998. Several other regimens are now available, including pertuzumab plus trastuzumab plus docetaxel, T-DM1, capecitabine plus lapatinib, and trastuzumab plus lapatinib. This study assesses the cost-effectiveness of four treatment sequences for HER-2-positive mBC according to the Taiwanese National Health Insurance Administration (TNHIA)., Methods: Costs (U.S. Dollars) and effectiveness (quality-adjusted life years) of four treatment sequences for HER-2-positive mBC patients were examined using a Markov model over a lifetime horizon. Transition probabilities, disease progression, and probability of adverse events and survival were derived from clinical trial data. Costs and health utilities were estimated from TNHIA, Taipei Medical University Hospital, and the literature. Deterministic, probabilistic sensitivity analyses and a scenario analysis examined parameter uncertainty and accounted for drug wastage in dosage and cost calculations., Results: Sequence 3 (1st line: trastuzumab plus docetaxel; 2nd line: T-DM1; 3rd line: trastuzumab plus lapatinib) was the most cost-effective sequence followed by sequence 1 (1st line: pertuzumab plus trastuzumab plus docetaxel; 2nd line: T-DM1; 3rd line: capecitabine plus lapatinib), and sequence 4 (1st line: trastuzumab plus docetaxel; 2nd line: trastuzumab plus lapatinib; 3rd line: trastuzumab plus capecitabine), respectively. The model was sensitive to costs and transition probabilities, but not particularly sensitive to the wastage assumption., Conclusions: From the perspective of the TNHIA, trastuzumab plus docetaxel as 1st line followed by T-DM1 and trastuzumab plus lapatinib as 2nd and 3rd line represents the most cost-effective strategy among the four sequences considered for treating HER-2-positive mBC patients., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

The present data article aims to describe the input parameters for a Markov model assessing the cost-effectiveness of four treatment sequences for patients with HER-2 positive metastatic breast cancer. The model input parameters include costs for physician visits, drugs, adverse event management, computed tomography (CT) scan, laboratory tests, echocardiogram, utilities, disutilities as well as the shape and scale parameters of a log-logistic distribution used for the transition probabilities., (© 2020 The Authors.)

Patients with pathogenic germline and somatic variants in DNA damage repair (DDR) genes may derive greater benefit with platinum-based chemotherapy in metastatic pancreatic ductal adenocarcinoma (PDAC). This study investigates the role of DDR genes as a predictive biomarker for response to first-line platinum chemotherapy with FOLFIRINOX in metastatic PDAC patients. Demographic, clinical, and pathologic variables were collected for patients with metastatic PDAC who received FOLFIRINOX as frontline treatment and who had germline and somatic genetic testing. Kaplan-Meier analysis of overall survival (OS) and progression free survival (PFS) were correlated to the presence of DDR pathogenic variants. Forty patients with metastatic PDAC met inclusion criteria. Germline genetic testing revealed germline pathogenic variants in DDR genes in 5 patients (12%), and somatic pathogenic variants in DDR genes in 4 patients (10%). Median PFS was significantly longer in patients with any (germline or somatic) pathogenic variant in DDR genes than in those without alterations 18.5 vs. 6.9 months (log-rank P=0.003). When restricted to the presence or absence of germline pathogenic variants in DDR genes, the median PFS was 18.5 vs. 7.4 months (log-rank P=0.005). The median OS for the entire cohort was 11.5 months was not statistically different between the two groups, however there were no deaths in the subgroup with germline pathogenic variants in DDR genes treated with frontline FOLFIRINOX. A subset of patients with metastatic PDAC and germline or somatic pathogenic variants in DDR genes have a statistically superior PFS when treated with the platinum containing regimen FOLFIRINOX. The role of DDR gene alterations as a predictive biomarker for FOLFIRINOX benefit should be further evaluated in prospective trials., Competing Interests: Conflicts of Interest: This was presented as a Poster presentation at the 2019 ASCO Gastrointestinal Cancers Symposium., (2019 Journal of Gastrointestinal Oncology. All rights reserved.)

Nanobodies (nAbs) are small, minimal antibodies that have distinct attributes that make them uniquely suited for certain biomedical research, diagnostic and therapeutic applications. Prominent uses include as intracellular antibodies or intrabodies to bind and deliver cargo to specific proteins and/or subcellular sites within cells, and as nanoscale immunolabels for enhanced tissue penetration and improved spatial imaging resolution. Here, we report the generation and validation of nAbs against a set of proteins prominently expressed at specific subcellular sites in mammalian brain neurons. We describe a novel hierarchical validation pipeline to systematically evaluate nAbs isolated by phage display for effective and specific use as intrabodies and immunolabels in mammalian cells including brain neurons. These nAbs form part of a robust toolbox for targeting proteins with distinct and highly spatially-restricted subcellular localization in mammalian brain neurons, allowing for visualization and/or modulation of structure and function at those sites., Competing Interests: JD, YL, MK, SP, CD, CM, RS, LS, TS, BG, KM, JT No competing interests declared, (© 2019, Dong et al.)

Halophytes are crucial in the light of increasing soil salinization, yet our understanding of their chemical composition and its relationship to key morphological traits such as succulence or salt excretion is limited. This study targets this issue by exploring the relationship between the elemental composition of 108 plant species from saline environments in Iran and their eco-morphological traits and taxonomy. Leaves and/or photosynthetic shoots of individual species and soils were sampled and analyzed for 20 elements in plant samples and 5 major elements plus % gypsum content, pH, and EC in soil samples. Eu-halophytes and leaf- and stem-succulent and salt-recreting plants showed high concentrations of Na, S, and Mg and low concentrations of Ca and K. In contrast, pseudo-halophytes, facultative-halophytes and eury-hygro-halophytes, which often lack succulent shoots, showed low Na, S, and Mg and high Ca and K concentrations in their leaves. Clear patterns were identified among taxonomic families, with Chenopodiaceae and Plumbaginaceae having high Na and Mg and low Ca and K concentrations, Caryophyllaceae having high K, Poaceae having low Na, and Asteraceae, Boraginaceae, and Brassicaceae showing high foliar Ca concentrations. We conclude that the elemental composition of halophytes and pseudo-halophytes is related to salt-tolerance categories, eco-morphological types and respective taxonomic groups., (Copyright © 2019 Elsevier Masson SAS. All rights reserved.)