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Advanced age represents one of the major risk factors for Parkinson's Disease. Recent biomedical studies posit a role for microRNAs, also known to be remodelled during ageing. However, the relationship between microRNA remodelling and ageing in Parkinson's Disease, has not been fully elucidated. Therefore, the aim of the present study is to unravel the relevance of microRNAs as biomarkers of Parkinson's Disease within the ageing framework. We employed Next Generation Sequencing to profile serum microRNAs from samples informative for Parkinson's Disease (recently diagnosed, drug-naïve) and healthy ageing (centenarians) plus healthy controls, age-matched with Parkinson's Disease patients. Potential microRNA candidates markers, emerging from the combination of differential expression and network analyses, were further validated in an independent cohort including both drug-naïve and advanced Parkinson's Disease patients, and healthy siblings of Parkinson's Disease patients at higher genetic risk for developing the disease. While we did not find evidences of microRNAs co-regulated in Parkinson's Disease and ageing, we report that hsa-miR-144-3p is consistently down-regulated in early Parkinson's Disease patients. Moreover, interestingly, functional analysis revealed that hsa-miR-144-3p is involved in the regulation of coagulation, a process known to be altered in Parkinson's Disease. Our results consistently show the down-regulation of hsa-mir144-3p in early Parkinson's Disease, robustly confirmed across a variety of analytical and experimental analyses. These promising results ask for further research to unveil the functional details of the involvement of hsa-mir144-3p in Parkinson's Disease., This work was supported by the Horizon 2020 Framework Programme (Grant number 634821, PROPAG-AGING).

Ground-based and airborne instrumentation for astronomy IX (2022), Montreal, JUL 17-22, 2022.--Proceedings of SPIE - The International Society for Optical Engineering vol. 12184 Article number 1218424.-- Complete list of authors: Marconi, A.; Abreu, M.; Adibekyan, V.; Alberti, V.; Albrecht, S.; Alcaniz, J.; Aliverti, M.; Allende Prieto, C.; Gomez, J. D. Alvarado; Amado, P. J.; Amate, M.; Andersen, M. I.; Artigau, E.; Baker, C.; Baldini, V.; Balestra, A.; Barnes, S. A.; Baron, F.; Barros, S. C. C.; Bauer, S. M.; Beaulieu, M.; Bellido-Tirado, O.; Benneke, B.; Bensby, T.; Bergin, E. A.; Biazzo, K.; Bik, A.; Birkby, J. L.; Blind, N.; Boisse, I.; Bolmont, E.; Bonaglia, M.; Bonfils, X.; Borsa, F.; Brandeker, A.; Brandner, W.; Broeg, C. H.; Brogi, M.; Brousseau, D.; Brucalassi, A.; Brynnel, J.; Buchhave, L. A.; Buscher, D. F.; Cabral, A.; Calderone, G.; Calvo-Ortega, R.; Cantalloube, F.; Canto Martins, B. L.; Carbonaro, L.; Chauvin, G.; Chazelas, B.; Cheffot, A. -L.; Cheng, Y. S.; Chiavassa, A.; Christensen, L.; Cirami, R.; Cook, N. J.; Cooke, R. J.; Coretti, I.; Covino, S.; Cowan, N.; Cresci, G.; Cristiani, S.; Cunha Parro, V.; Cupani, G.; D'Odorico, V.; de Castro Leao, I.; De Cia, A.; De Medeiros, J. R.; Debras, F.; Debus, M.; Demangeon, O.; Dessauges-Zavadsky, M.; Di Marcantonio, P.; Dionies, F.; Doyon, R.; Dunn, J.; Ehrenreich, D.; Faria, J. P.; Feruglio, C.; Fisher, M.; Fontana, A.; Fumagalli, M.; Fusco, T.; Fynbo, J.; Gabella, O.; Gaessler, W.; Gallo, E.; Gao, X.; Genolet, L.; Genoni, M.; Giacobbe, P.; Giro, E.; Goncalves, R. S.; Gonzalez, O. A.; Gonzalez Hernandez, J. I.; Gracia Temich, F.; Haehnelt, M. G.; Haniff, C.; Hatzes, A.; Helled, R.; Hoeijmakers, H. J.; Huke, P.; Jaervinen, A. S.; Jaervinen, S. P.; Kaminski, A.; Korn, A. J.; Kouach, D.; Kowzan, G.; Kreidberg, L.; Landoni, M.; Lanotte, A.; Lavail, A.; Li, J.; Liske, J.; Lovis, C.; Lucatello, S.; Lunney, D.; MacIntosh, M. J.; Madhusudhan, N.; Magrini, L.; Maiolino, R.; Malo, L.; Man, A. W. S.; Marquart, T.; Marques, E. L.; Martins, C. J. A. P.; Martins, A. M.; Maslowski, P.; Mason, E.; Mason, C. A.; McCracken, R. A.; Mergo, P.; Micela, G.; Mitchell, T.; Molliere, P.; Monteiro, M. A.; Montgomery, D.; Mordasini, C.; Morin, J.; Mucciarelli, A.; Murphy, M. T.; N'Diaye, M.; Neichel, B.; Niedzielski, A. T.; Niemczura, E.; Nortmann, L.; Noterdaeme, P.; Nunes, N. J.; Oggioni, L.; Oliva, E.; Onel, H.; Origlia, L.; Ostlin, G.; Palle, E.; Papaderos, P.; Pariani, G.; Penate Castro, J.; Pepe, F.; Levasseur, L. Perreault; Petit, P.; Pino, L.; Piqueras, J.; Pollo, A.; Poppenhaeger, K.; Quirrenbach, A.; Rauscher, E.; Rebolo, R.; Redaelli, E. M. A.; Reffert, S.; Reid, D. T.; Reiners, A.; Richter, P.; Riva, M.; Rivoire, S.; Rodriguez-Lopez, C.; Roederer, I. U.; Romano, D.; Rousseau, S.; Rowe, J.; Salvadori, S.; Sanna, N.; Santos, N. C.; Diaz, P. Santos; Sanz-Forcada, J.; Sarajlic, M.; Sauvage, J. -F.; Schaefer, S.; Schiavon, R. P.; Schmidt, T. M.; Selmi, C.; Sivanandam, S.; Sordet, M.; Sordo, R.; Sortino, F.; Sosnowska, D.; Sousa, S. G.; Stempels, E.; Strassmeier, K. G.; Suarez Mascareno, A.; Sulich, A.; Sun, X.; Tanvir, N. R.; Tenegi-Sangines, F.; Thibault, S.; Thompson, S. J.; Tozzi, A.; Turbet, M.; Vallee, P.; Varas, R.; Venn, K. A.; Veran, J. -P.; Verma, A.; Viel, M.; Wade, G.; Waring, C.; Weber, M.; Weder, J.; Wehbe, B.; Weingrill, J.; Woche, M.; Xompero, M.; Zackrisson, E.; Zanutta, A.; Zapatero Osorio, M. R.; Zechmeister, M.; Zimara, J., The first generation of ELT instruments includes an optical-infrared high resolution spectrograph, indicated as ELT-HIRES and recently christened ANDES (ArmazoNes high Dispersion Echelle Spectrograph). ANDES consists of three fibre-fed spectrographs (UBV, RIZ, YJH) providing a spectral resolution of similar to 100,000 with a minimum simultaneous wavelength coverage of 0.4-1.8 mu m with the goal of extending it to 0.35-2.4 mu m with the addition of a K band spectrograph. It operates both in seeing- and diffraction-limited conditions and the fibre-feeding allows several, interchangeable observing modes including a single conjugated adaptive optics module and a small diffraction-limited integral field unit in the NIR. Its modularity will ensure that ANDES can be placed entirely on the ELT Nasmyth platform, if enough mass and volume is available, or partly in the Coude room. ANDES has a wide range of groundbreaking science cases spanning nearly all areas of research in astrophysics and even fundamental physics. Among the top science cases there are the detection of biosignatures from exoplanet atmospheres, finding the fingerprints of the first generation of stars, tests on the stability of Nature's fundamental couplings, and the direct detection of the cosmic acceleration. The ANDES project is carried forward by a large international consortium, composed of 35 Institutes from 13 countries, forming a team of more than 200 scientists and engineers which represent the majority of the scientific and technical expertise in the field among ESO member states., The Italian effort for ANDES is supported by the Italian National Institute for Astrophysics (INAF). The Portuguese participation is supported by FCT -Fundacao para a Ciencia e a Tecnologia through national funds and by FEDER through COMPETE2020 -Programa Operacional Competitividade e Internacionalizacao by these grants: UID/FIS/04434/2019, UIDB/04434/2020 & UIDP/04434/2020; POCI-01-0145-FEDER-032113 & PTDC/FIS-AST/32113/2017. Swedish participation in the ANDES project is made possible through the national Swedish ELT Instrumentation Consortium (SELTIC), suppored by the Swedish Research Council (VR). CJM acknowledges FCT and POCH/FSE (EC) support through Investigador FCT Contract 2021.01214.CEECIND/CP1658/CT0001. JLB acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program under grant agreement No 805445. MTM acknowledges the support of the Australian Research Council through Future Fellowship grant FT180100194 SS acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program under grant agreement No 804240. TMS acknowledgment the support from the SNF synergia grant CRSII5-193689 (BLUVES), With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709

BACKGROUND AND AIMS: Obesity is a key contributing factor to incidental type 2 diabetes and cardiovascular disease. CXCR3 receptor and its ligands CXCL 10 and 11 are associated with atherosclerosis and cardiovascular disease. The aim of our study was to analyse the role of the CXCR3 ligands on insulin resistance (IR) and endothelial dysfunction in human obesity. METHODS AND RESULTS: We have studied 45 obese patients (mean age 44?±?6?years, body mass index 45?±?9?kg/m2) who were selected for Roux-Y-gastric bypass surgery and 21 non obese control subjects with similar age and gender distribution. We measured by ELISA the circulating levels of the CXCR3 ligands interferon-gamma inducible protein 10 (IP-10/CXCL10) and interferon-gamma-inducible T-cell alpha chemoattractant (I-TAC/CXCL11). Using an ex vivo procedure with the flow chamber assay, we have investigated the effect of such chemokines on endothelial leukocytes arrest under dynamic conditions. Peripheral blood levels of CXCL10 and CXCL11 were significantly higher in obese subjects than in controls (p?0.001) and significantly correlated with BMI, waist circunference and HOMA-IR. Obese patients with HOMA-IR index above 75th percentile showed highest increase of circulating CXCL10 and CXCL11 values. Under dynamic flow conditions, the enhanced adhesion of patient leukocytes to TNFalpha-induced human arterial endothelial cells was partly dependent on CXCR3. CONCLUSIONS: The study demonstrates that CXCL10 and CXCL11 are associated with IR and enhance leukocyte endothelial arrest in obese subjects. Blockade of CXCR3 signaling might be a new therapeutic approach for the prevention of obesity-associated cardiovascular co-morbidities.

Canine parvovirus type 2 (CPV2) is one of the most important intestinal pathogens in dogs and puppies. CPV2 has been evolved into three genetic and antigenic variants (2a, 2b, and 2c), which are distributed worldwide. We reported the first study of genetic diversity of CPV2 in Chile. Sixty-five samples were collected from puppies presenting with severe gastroenteritis and different vaccination statuses. PCR, restriction fragment length polymorphism (RFLP), and partial sequencing of the coding region of the structural viral protein VP2 was performed. Thirty of a total of 65 samples tested positive by PCR out of which 19 were further classified as CPV2c and one as CPV2a using RFLP and Sanger sequencing. The phylogeny was in concordance with the RFLP analysis. This is the first report of the genetic characterization of CPV2 in Chile and reveals a high occurrence of CPV2c.

International audience; HIRES will be the high-resolution spectrograph at optical and near-infrared (NIR) wavelengths for ESO's Extremely Large Telescope (ELT). It will consist of three fibre-fed spectrographs providing a wavelength coverage of 0.4–1.8 µm (with a goal of 0.35–1.8 µm) at a spectral resolution of ~ 100 000. Fibre-feeding allows HIRES to have several interchangeable observing modes, including a single-conjugate adaptive optics (SCAO) module and a small diffraction-limited integral field unit in the NIR. It will therefore be able to operate in both seeing- and diffraction-limited modes. HIRES will address a wide range of science cases spanning nearly all areas of research in astrophysics and even fundamental physics. Some of the top science cases will be the detection of biosignatures from exoplanet atmospheres, finding the fingerprints of the first generation of stars (Pop III), tests on the stability of Nature's fundamental couplings, and the direct detection of the cosmic acceleration. The HIRES consortium is composed of more than 30 institutes from 14 countries, forming a team of more than 200 scientists and engineers.