196 results on '"Oh, Hyun Woo"'
Search Results
152. A Control Plane of Premium IP Multicast
- Author
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Oh, Hyun Woo, primary, Cho, Kee Seong, additional, and Kim, Sang Ha, additional
- Published
- 2006
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153. Stenotrophomonas dokdonensis sp. nov., isolated from soil
- Author
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Yoon, Jung-Hoon, primary, Kang, So-Jung, additional, Oh, Hyun Woo, additional, and Oh, Tae-Kwang, additional
- Published
- 2006
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154. Porphyrobacter dokdonensis sp. nov., isolated from sea water
- Author
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Yoon, Jung-Hoon, primary, Kang, So-Jung, additional, Lee, Mi-Hwa, additional, Oh, Hyun Woo, additional, and Oh, Tae-Kwang, additional
- Published
- 2006
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155. Brevundimonas kwangchunensis sp. nov., isolated from an alkaline soil in Korea
- Author
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Yoon, Jung-Hoon, primary, Kang, So-Jung, additional, Oh, Hyun Woo, additional, Lee, Jung-Sook, additional, and Oh, Tae-Kwang, additional
- Published
- 2006
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156. Antioxidant Effects of Quinoline Alkaloids and 2,4-Di-tert-butylphenol Isolated from Scolopendra subspinipes
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Yoon, Mi-Ae, primary, Jeong, Tae-Sook, additional, Park, Doo-Sang, additional, Xu, Ming-Zhe, additional, Oh, Hyun-Woo, additional, Song, Kyoung-Bin, additional, Lee, Woo Song, additional, and Park, Ho-Yong, additional
- Published
- 2006
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157. Cyclobacterium amurskyense sp. nov., a novel marine bacterium isolated from sea water
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Nedashkovskaya, Olga I., primary, Kim, Seung Bum, additional, Lee, Myung Sook, additional, Park, Myung Soo, additional, Lee, Kang Hyun, additional, Lysenko, Anatoly M., additional, Oh, Hyun Woo, additional, Mikhailov, Valery V., additional, and Bae, Kyung Sook, additional
- Published
- 2005
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158. Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003
- Author
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Nedashkovskaya, Olga I., primary, Kim, Seung Bum, additional, Suzuki, Makoto, additional, Shevchenko, Lyudmila S., additional, Lee, Myung Sook, additional, Lee, Kang Hyun, additional, Park, Myung Soo, additional, Frolova, Galina M., additional, Oh, Hyun Woo, additional, Bae, Kyung Sook, additional, Park, Ho-Yong, additional, and Mikhailov, Valery V., additional
- Published
- 2005
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159. Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea
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Yoon, Jung-Hoon, primary, Kang, So-Jung, additional, Lee, Choong-Hwan, additional, Oh, Hyun Woo, additional, and Oh, Tae-Kwang, additional
- Published
- 2005
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160. Paenibacillus elgii sp. nov., with broad antimicrobial activity
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Kim, Dal-Soo, primary, Bae, Cheol-Yong, additional, Jeon, Jae-Jin, additional, Chun, Sam-Jae, additional, Oh, Hyun Woo, additional, Hong, Soon Gyu, additional, Baek, Keun-Sik, additional, Moon, Eun Young, additional, and Bae, Kyung Sook, additional
- Published
- 2004
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161. Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments
- Author
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Yi, Hana, primary, Chang, Young-Hyo, additional, Oh, Hyun Woo, additional, Bae, Kyung Sook, additional, and Chun, Jongsik, additional
- Published
- 2003
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162. Paenibacillus daejeonensis sp. nov., a novel alkaliphilic bacterium from soil.
- Author
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Lee, Jung-Sook, primary, Lee, Keun Chul, additional, Chang, Young-Hyo, additional, Hong, Soon Gyu, additional, Oh, Hyun Woo, additional, Pyun, Yu-Ryang, additional, and Bae, Kyung Sook, additional
- Published
- 2002
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163. Protein purification and nucleotide sequence of a lysozyme from the bacteria-induced larvae of the fall webworm,Hyphantria cunea
- Author
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Park, Ho-Yong, primary, Park, Soon Sik, additional, Shin, Sang Woon, additional, Park, Doo-Sang, additional, Kim, Mi Gwang, additional, Oh, Hyun Woo, additional, and Joo, Chang Kyeong, additional
- Published
- 1997
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164. Induction of clusterinExpression by Neuronal Cell Death in Zebrafish
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Jeong, Yun-Mi, Jin, Tae-Eun, Choi, Jung-Hwa, Lee, Mi-Sun, Kim, Hyun-Taek, Hwang, Kyu-Seok, Park, Doo-Sang, Oh, Hyun-Woo, Choi, Joong-Kook, Korzh, Vladimir, Schachner, Melitta, You, Kwan-Hee, and Kim, Cheol-Hee
- Abstract
Clusterin, a protein associated with multiple functions, is expressed in a wide variety of mammalian tissues. Although clusterin is known to be involved in neurodegenerative diseases, ageing, and tumorigenesis, a detailed analysis of the consequences of gain- or loss-of-function approaches has yet to be performed to understand the underlying mechanisms of clusterin functions. Since clusterin levels change in neurological diseases, it is likely that clusterin contributes to cell death and degeneration in general. Zebrafish was investigated as a model system to study human diseases. During development, zebrafish clusterinwas expressed in the notochord and nervous system. Embryonic overexpression of clusterinby mRNA microinjection did not affect axis formation, whereas its knock-down by anti-sense morpholino treatment resulted in neuronal cell death. To analyze the function of clusterinin neurodegeneration, a transgenic zebrafish was investigated, in which nitroreductase expression is regulated under the control of a neuron-specific huCpromoter which is active between the stages of early neuronal precursors and mature neurons. Nitroreductase turns metronidazole into a cytotoxic agent that induces cell death within 12 h. After metronidazole treatment, transgenic zebrafish showed neuron-specific cell death. Interestingly, we also observed a dramatic induction of clusterinexpression in the brain and spinal cord in these fish, suggesting a direct or indirect role of clusterinin neuronal cell death and thus, more generally, in neurodegeneration.
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- 2014
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165. LAMMER Kinase LkhA Plays Multiple Roles in the Vegetative Growth and Asexual and Sexual Development of Aspergillus nidulans.
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Kang, Eun-Hye, Kim, Ji-ae, Oh, Hyun-Woo, and Park, Hee-Moon
- Subjects
PROTEIN kinases ,PLANT growth ,ASPERGILLUS nidulans ,FUNGAL growth ,SEX (Biology) ,EUKARYOTES ,FILAMENTOUS fungi ,MOLECULAR biology - Abstract
LAMMER kinase plays pivotal roles in various physiological processes in eukaryotes; however, its function in filamentous fungi is not known. We performed molecular studies on the function of the Aspergillus nidulans LAMMER kinase, LkhA, and report its involvement in multiple developmental processes. The gene for LkhA was highly expressed during reproductive organ development, such as that of conidiophores and cleistothecia. During vegetative growth, the patterns of germ tube emergence and hyphal polarity were changed and septation was increased by lkhA deletion. Northern analyses showed that lkhA regulated the transcription of brlA, csnD, and ppoA, which supported the detrimental effect of lkhA-deletion on asexual and sexual differentiation. LkhA also affected expression of cyclin-dependent kinase NimX
cdc2 , a multiple cell cycle regulator, and StuA, an APSES family of fungal transcription factors that play pivotal roles in multiple differentiation processes. Here, for the first time, we present molecular evidence showing that LAMMER kinase is involved in A. nidulans development by modulating the expression of key regulators of developmental processes. [ABSTRACT FROM AUTHOR]- Published
- 2013
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166. Protein purification and nucleotide sequence of a lysozyme from the bacteria-induced larvae of the fall webworm, Hyphantria cunea.
- Author
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Park, Ho-Yong, Park, Soon Sik, Shin, Sang Woon, Park, Doo-Sang, Kim, Mi Gwang, Oh, Hyun Woo, and Joo, Chang Kyeong
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- 1997
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167. Protein purification and cDNA cloning of a cecropin-like peptide from the larvae of fall webworm (Hyphantria cunea)
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Park, Soon Sik, Shin, Sang Woon, Park, D.-S., Oh, Hyun Woo, Boo, Kyung Saeng, and Park, H.-Y.
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- 1997
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168. Characterization of Elastic Polymer-Based Smart Insole and a Simple Foot Plantar Pressure Visualization Method Using 16 Electrodes.
- Author
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Lee, Wangjoo, Hong, Seung-Hyeon, and Oh, Hyun-Woo
- Subjects
ELECTRODES ,DETECTORS ,PRESSURE sensors ,CARBON nanotubes ,MANUFACTURING processes - Abstract
In this paper, we propose a smart insole for inexpensive plantar pressure sensing and a simple visualizing scheme. The insole is composed of two elastomeric layers and two electrode layers where the common top electrode is submerged in the insole. The upper elastomeric layer is non-conductive poly-dimethyl-siloxane (PDMS) and supports plantar pressure buffering and the lower layer is carbon nano-tube (CNT)-dispersed PDMS for pressure sensing through piezo-resistivity. Under the lower sensing layer are 16 bottom electrodes for pressure distribution sensing without cell-to-cell interference. Since no soldering or sewing is needed the smart insole manufacturing processes is simple and cost-effective. The pressure sensitivity and time response of the material was measured and based on the 16 sensing data of the smart insole, we virtually extended the frame size for continuous and smoothed pressure distribution image with the help of a simple pseudo interpolation scheme. [ABSTRACT FROM AUTHOR]
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- 2019
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169. LAMMER Kinase LkhA Plays Multiple Roles in the Vegetative Growth and Asexual and Sexual Development of Aspergillus nidulans.
- Author
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Kang, Eun-Hye, Kim, Ji-ae, Oh, Hyun-Woo, and Park, Hee-Moon
- Subjects
- *
PROTEIN kinases , *PLANT growth , *ASPERGILLUS nidulans , *FUNGAL growth , *SEX (Biology) , *EUKARYOTES , *FILAMENTOUS fungi , *MOLECULAR biology - Abstract
LAMMER kinase plays pivotal roles in various physiological processes in eukaryotes; however, its function in filamentous fungi is not known. We performed molecular studies on the function of the Aspergillus nidulans LAMMER kinase, LkhA, and report its involvement in multiple developmental processes. The gene for LkhA was highly expressed during reproductive organ development, such as that of conidiophores and cleistothecia. During vegetative growth, the patterns of germ tube emergence and hyphal polarity were changed and septation was increased by lkhA deletion. Northern analyses showed that lkhA regulated the transcription of brlA, csnD, and ppoA, which supported the detrimental effect of lkhA-deletion on asexual and sexual differentiation. LkhA also affected expression of cyclin-dependent kinase NimXcdc2, a multiple cell cycle regulator, and StuA, an APSES family of fungal transcription factors that play pivotal roles in multiple differentiation processes. Here, for the first time, we present molecular evidence showing that LAMMER kinase is involved in A. nidulans development by modulating the expression of key regulators of developmental processes. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
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170. Species-Specific Interactions between Plant Metabolites and Insect Juvenile Hormone Receptors.
- Author
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Shin SW, Jeon JH, Yun CS, Jeong SA, Kim JA, Park DS, Shin Y, and Oh HW
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- Animals, Diterpenes chemistry, Diterpenes metabolism, Diterpenes pharmacology, Evolution, Molecular, Insecta growth & development, Insecticides metabolism, Insecticides toxicity, Juvenile Hormones metabolism, Larva drug effects, Larva growth & development, Larva metabolism, Nuclear Receptor Coactivators metabolism, Plant Extracts chemistry, Plants chemistry, Protein Binding, Species Specificity, Insecta metabolism, Plants metabolism
- Abstract
Because juvenile hormone (JH) controls insect development and its analogs are used as insecticides, juvenile hormone disruptors (JHDs) represent potential sources from which novel pesticides can be developed. Many plant species harbor JHD activity, which has previously been attributed plant secondary metabolites (i.e., diterpenes) that disrupt insect development by interfering with the JH-mediated heterodimer formation of insect juvenile receptor complexes. The results of the present study indicate that plant JHD activity is also concentrated in certain plant groups and families and that plant metabolites have insect group-specific activity. These findings suggest that reciprocal diversification has occurred between plants and insects through the evolution of the plant metabolites and JH receptors, respectively, and that plant metabolites could be developed into insect group-specific pesticides with limited effects on non-target species.
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- 2018
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171. Conifer Diterpene Resin Acids Disrupt Juvenile Hormone-Mediated Endocrine Regulation in the Indian Meal Moth Plodia interpunctella.
- Author
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Oh HW, Yun CS, Jeon JH, Kim JA, Park DS, Ryu HW, Oh SR, Song HH, Shin Y, Jung CS, and Shin SW
- Subjects
- Abietanes metabolism, Animals, Pinus physiology, Diterpenes metabolism, Herbivory, Juvenile Hormones metabolism, Moths physiology, Plant Extracts metabolism, Tracheophyta physiology
- Abstract
Diterpene resin acids (DRAs) are important components of oleoresin and greatly contribute to the defense strategies of conifers against herbivorous insects. In the present study, we determined that DRAs function as insect juvenile hormone (JH) antagonists that interfere with the juvenile hormone-mediated binding of the JH receptor Methoprene-tolerant (Met) and steroid receptor coactivator (SRC). Using a yeast two-hybrid system transformed with Met and SRC from the Indian meal moth Plodia interpunctella, we tested the interfering activity of 3704 plant extracts against JH III-mediated Met-SRC binding. Plant extracts from conifers, especially members of the Pinaceae, exhibited strong interfering activity, and four active interfering DRAs (7α-dehydroabietic acid, 7-oxodehydroabietic acid, dehydroabietic acid, and sandaracopimaric acid) were isolated from roots of the Japanese pine Pinus densiflora. The four isolated DRAs, along with abietic acid, disrupted the juvenile hormone-mediated binding of P. interpunctella Met and SRC, although only 7-oxodehydroabietic acid disrupted larval development. These results demonstrate that DRAs may play a defensive role against herbivorous insects via insect endocrine-disrupting activity.
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- 2017
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172. Hyunsoonleella udoensis sp. nov., isolated from a gravel sample from a beach of Udo island, Korea.
- Author
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Kim H, Lee JB, Oh HW, Cha HK, Yang JH, Bae KS, and Park DS
- Subjects
- Aerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol chemistry, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Fatty Acids analysis, Hydrogen-Ion Concentration, Islands, Korea, Molecular Sequence Data, Phospholipids analysis, Phylogeny, Quinones analysis, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Sodium Chloride metabolism, Temperature, Environmental Microbiology, Flavobacteriaceae classification, Flavobacteriaceae isolation & purification
- Abstract
A Gram-stain negative, non-motile, rod-shaped and aerobic bacterial strain, designated JG48(T), was isolated from a gravel sample taken from a beach adjacent to Udo island, South Korea. Strain JG48(T) was found to grow optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. The 16S rRNA gene sequence of strain JG48(T) exhibited sequence similarities of 96.67 % to Hyunsoonleella jejuensis CNU004(T). The major fatty acids present in the strain JG48(T) were identified as iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and iso-C15:0 3-OH. The predominant isoprenoid quinone was identified as MK-6. The polar lipids profile of strain JG48(T) was found to consist of phosphatidylethanolamine, three unidentified amino lipids and four unidentified lipids. The DNA G+C content of strain JG48(T) was determined to be 34 mol%. Based on the morphological and physiological properties, and the results of phylogenetic analyses, the strain is considered to represent a novel species of the genus Hyunsoonleella, for which the name Hyunsoonleella udoensis sp. nov. is proposed. The type strain is JG48(T) (=KCTC 42341(T)=JCM 30600(T)).
- Published
- 2015
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173. Identification of plant compounds that disrupt the insect juvenile hormone receptor complex.
- Author
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Lee SH, Oh HW, Fang Y, An SB, Park DS, Song HH, Oh SR, Kim SY, Kim S, Kim N, Raikhel AS, Je YH, and Shin SW
- Subjects
- Animals, Diterpenes isolation & purification, Insecta growth & development, Larva drug effects, Larva growth & development, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Two-Hybrid System Techniques, Diterpenes pharmacology, Herbivory drug effects, Insect Proteins metabolism, Insecta drug effects, Juvenile Hormones antagonists & inhibitors, Lindera chemistry, Solidago chemistry
- Abstract
Insects impact human health through vector-borne diseases and cause major economic losses by damaging crops and stored agricultural products. Insect-specific growth regulators represent attractive control agents because of their safety to the environment and humans. We identified plant compounds that serve as juvenile hormone antagonists (PJHANs). Using the yeast two-hybrid system transformed with the mosquito JH receptor as a reporter system, we demonstrate that PJHANs affect the JH receptor, methoprene-tolerant (Met), by disrupting its complex with CYCLE or FISC, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants: Lindera erythrocarpa and Solidago serotina. They are effective in causing mortality of mosquito larvae at relatively low LD50 values. Topical application of two diterpenes caused reduction in the expression of Met target genes and retardation of follicle development in mosquito ovaries. Hence, the newly discovered PJHANs may lead to development of a new class of safe and effective pesticides.
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- 2015
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174. Antarctobacter jejuensis sp. nov., isolated from seawater in Jeju, Korea.
- Author
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Kim H, Lee JB, Oh HW, Lee H, Shin KS, Bae KS, and Park DS
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- Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol chemistry, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Fatty Acids analysis, Hydrogen-Ion Concentration, Korea, Locomotion, Molecular Sequence Data, Phospholipids analysis, Phylogeny, Quinones analysis, RNA, Ribosomal, 16S genetics, Rhodobacteraceae genetics, Rhodobacteraceae physiology, Sequence Analysis, DNA, Sodium Chloride metabolism, Temperature, Rhodobacteraceae classification, Rhodobacteraceae isolation & purification, Seawater microbiology
- Abstract
A novel bacterium, designated strain 13-2-B6(T), was isolated from seawater adjacent to Songak Mountain on Jeju Island, South Korea. The novel strain was observed to be Gram-negative, aerobic, rod-shaped and motile with a single polar flagellum. On the basis of 16S rRNA gene sequence similarity, strain 13-2-B6(T) was determined to be phylogenetically closely related to the type strain of Antarctobacter heliothermus, currently the sole species of the genus Antarctobacter (family Rhodobacteraceae). Sequence similarity between the 16S rRNA genes of strain 13-2-B6(T) and A. heliothermus EL-219(T) is 96.9 %. Strain 13-2-B6(T) was found to grow optimally at 25-30 °C, pH 7.0-8.0 and 3 % (w/v) NaCl. The predominant isoprenoid quinone in strain 13-2-B6(T) was identified as ubiquinone Q-10 and the major fatty acids were identified as C18:1 ω7c and/or C18:1 ω6c. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unknown aminolipids, two unknown phospholipids, an unknown glycolipid and an unknown lipid were found to be components of the polar lipid profile. The G + C content of strain 13-2-B6(T) was determined to be 62 mol %. On the basis of its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain 13-2-B6(T) is considered to represent a novel species of the genus Antarctobacter, for which the name Antarctobacter jejuensis sp. nov. is proposed. The type strain is 13-2-B6(T) (=KCTC 42009(T) =JCM 19898(T)).
- Published
- 2014
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175. Establishment of a transgenic zebrafish EF1α:Kaede for monitoring cell proliferation during regeneration.
- Author
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Moon HY, Kim OH, Kim HT, Choi JH, Yeo SY, Kim NS, Park DS, Oh HW, You KH, De Zoysa M, and Kim CH
- Subjects
- Amputation, Surgical, Animal Fins embryology, Animal Fins metabolism, Animals, Animals, Genetically Modified embryology, Animals, Genetically Modified genetics, Animals, Genetically Modified growth & development, Animals, Genetically Modified metabolism, Embryo, Nonmammalian embryology, Embryo, Nonmammalian metabolism, Gene Expression Regulation, Developmental, Luminescent Proteins genetics, Luminescent Proteins metabolism, Organ Specificity, Peptide Elongation Factor 1 genetics, Zebrafish embryology, Zebrafish genetics, Zebrafish metabolism, Zebrafish Proteins genetics, Animal Fins growth & development, Cell Proliferation, Peptide Elongation Factor 1 metabolism, Wound Healing, Zebrafish growth & development, Zebrafish Proteins metabolism
- Abstract
Zebrafish is considered as a versatile experimental animal for various research models from development to diseases. In this study, we report the development of transgenic zebrafish line named as Tg(EF1α:Kaede) that expresses translation elongation factor 1 subunit alpha (EF1α) promoter linked to a fluorescent protein (FP), Kaede for monitoring proliferating cells in during regeneration. It was revealed that about 1.4 kb 5'-flanking region of the EF1α was sufficient for its promoter activity. Expression of Kaede with a property of photo-conversion from green to red was detected in different embryonic stages as well as various organs such as brain, heart, pancreas, intestine, ovary, and fins of adult fish. Cell proliferation pattern during fin regeneration was monitored after amputation of Tg(EF1α:Kaede) caudal fin and results shown that this system is simple and efficient method for detecting proliferating cells during tissue regeneration. Developed Tg(EF1α:Kaede) line has potential to investigate the cell proliferation, regeneration, wound healing capacities after tissue damage and evaluate the therapeutic power of wound healing drugs., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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176. Draft genome sequence of the novel enteric bacterium Galloisinimonas intestini B14T KCTC 32180, isolated from the gut of a Galloisiana species (Notoptera: Grylloblattidae) fossil insect.
- Author
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Park DS, Bae KS, Kim H, Shin KS, Choi SH, Kim DS, Kim BW, and Oh HW
- Subjects
- Animals, Enterobacteriaceae isolation & purification, Fossils, Gastrointestinal Tract microbiology, Insecta microbiology, Korea, Molecular Sequence Data, DNA, Bacterial chemistry, DNA, Bacterial genetics, Enterobacteriaceae genetics, Genome, Bacterial, Sequence Analysis, DNA
- Abstract
We report the 3.74-Mb genome sequence of Galloisinimonas intestini B14(T), isolated from the gut of one of the world's rarest insect species, Galloisiana sp., collected at a Mosan cave, Moonkyung, Gyungsangbook-do, South Korea. Strain B14(T) is a novel genus candidate of the family Enterobacteriaceae.
- Published
- 2012
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177. Phycicoccus ochangensis sp. nov., isolated from soil of a potato cultivation field.
- Author
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Kim H, Oh HW, Park DS, Lee KH, Kim SU, Park HM, and Bae KS
- Subjects
- Actinomycetales classification, Actinomycetales genetics, Actinomycetales metabolism, Fatty Acids metabolism, Molecular Sequence Data, Phylogeny, Sodium Chloride metabolism, Solanum tuberosum growth & development, Solanum tuberosum microbiology, Actinomycetales isolation & purification, Soil Microbiology
- Abstract
Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9(T) and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10-45°C, pH 5.0-10.0, and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-8(H(4)) and the main cellular fatty acids were iso-C(14:0), iso-C(15:0), and anteiso-C(15:0). Polar lipids in strain L1b-b9(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9(T) and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9(T) is related most closely to Phycicoccus cremeus V2M29(T) (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9(T) (=KCTC 19695(T) [corrected] =JCM 17595(T)).
- Published
- 2012
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178. Methylobacterium dankookense sp. nov., isolated from drinking water.
- Author
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Lee SW, Oh HW, Lee KH, and Ahn TY
- Subjects
- Aerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Fatty Acids analysis, Hydrogen-Ion Concentration, Methylobacterium chemistry, Methylobacterium genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones analysis, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Temperature, Fresh Water microbiology, Methylobacterium classification, Methylobacterium isolation & purification
- Abstract
A pink-pigmented bacterium, designated SW08-7(T) was isolated from the drinking water of a water purifier. Cells were Gram-negative, rod-shaped, strictly aerobic, and non-spore-forming. It grew optimally at 25 degrees C, pH 6 approximately 7. Phylogenese analysis based on 16S rRNA gene sequence showed that strain SW08-7(T) belongs to the genus Methylobacterium. The highest 16S rRNA gene sequence similarities were found to Methylobacterium mesophilicum JCM 2829(T) (96.9%), Methylobacterium brachiatum B0021(T) (96.9%), Methylobacterium phyllosphaerae CBMB27(T) (96.6%), Methylobacterium radiotolerans JCM 2831(T) (96.6%), and Methylobacterium hispanicum GP34(T) (96.5%). DNA-DNA hybridization experiment revealed low-level (28.5%) of DNA-DNA relatedness between strain SW08-7(T) and Methylobacterium hispanicum. The genomic DNA G+C content was 68.9 mol% and the major isoprenoid quinone was Q-10. The major cellular fatty acid of strain SW08-7(T) was C(18:1) omega7c (79.8+/-2.1%). Results of phylogenetic, phenotypic, and biochemical analyses revealed that strain SW08-7(T) could be classified as representing a novel species of genus Methylobacterium, for which the name Methylobacterium dankookense sp. nov. is proposed. The type strain is SW08-7(T) (=KCTC 22512(T) =DSM 22415(1)).
- Published
- 2009
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179. Dokdonella ginsengisoli sp. nov., isolated from soil from a ginseng field, and emended description of the genus Dokdonella.
- Author
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Ten LN, Jung HM, Im WT, Oh HW, Yang DC, Yoo SA, and Lee ST
- Subjects
- Aerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Fatty Acids analysis, Korea, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Panax, Phylogeny, Quinones analysis, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Xanthomonadaceae genetics, Xanthomonadaceae physiology, Soil Microbiology, Xanthomonadaceae classification, Xanthomonadaceae isolation & purification
- Abstract
A Gram-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 191T, was isolated from a soil sample from a ginseng field in Pocheon Province, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 191T belongs to the family Xanthomonadaceae and is related to Dokdonella fugitiva LMG 23001T (97.8% sequence similarity) and Dokdonella koreensis KCTC 12396T (96.9%). The G+C content of the genomic DNA was 68.7 mol%. The major respiratory quinone was Q-8 and the major fatty acids were iso-C17:1omega9c (30.6%), iso-C17:0 (21.6%) and iso-C15:0 (13.0%), supporting the affiliation of strain Gsoil 191T to the genus Dokdonella. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness values between strain Gsoil 191T and its closest phylogenetic neighbours were below 40%. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Gsoil 191T from recognized species of the genus Dokdonella. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 191T represents a novel species of the genus Dokdonella, for which the name Dokdonella ginsengisoli sp. nov. is proposed. The type strain is Gsoil 191T (=KCTC 12564T=DSM 17954T=CCUG 52462T).
- Published
- 2009
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180. Paenibacillus pectinilyticus sp. nov., isolated from the gut of Diestrammena apicalis.
- Author
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Park DS, Jeong WJ, Lee KH, Oh HW, Kim BC, Bae KS, and Park HY
- Subjects
- Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial analysis, DNA, Ribosomal analysis, Genotype, Gram-Positive Endospore-Forming Rods genetics, Gram-Positive Endospore-Forming Rods isolation & purification, Gram-Positive Endospore-Forming Rods physiology, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Gastrointestinal Tract microbiology, Gram-Positive Endospore-Forming Rods classification, Orthoptera microbiology
- Abstract
During a search for exo-enzyme-producing bacteria in the gut of an insect, Diestrammena apicalis, a novel bacterium capable of degrading pectin was isolated. The isolate, designated strain RCB-08(T), comprised Gram-positive, endospore-forming, motile rods capable of growth at 15-30 degrees C and pH 6.0-8.7. The DNA G+C content of the isolate was 51.5 mol% and the predominant cellular fatty acid was anteiso-C(15 : 0) (74.1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RCB-08(T) was affiliated with a cluster within the Paenibacillaceae, and was related most closely to Paenibacillus chondroitinus NBRC 15376(T), with a sequence similarity of 96.7 %. The DNA-DNA relatedness value for strain RCB-08(T) with P. chondroitinus NBRC 15376(T) was 15.0 %. Strain RCB-08(T) hydrolysed pectin, but not cellulose, casein, starch or xylan. Strain RCB-08(T) could be clearly distinguished from other Paenibacillus species on the basis of characteristics observed using a polyphasic approach. Therefore strain RCB-08(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus pectinilyticus sp. nov. is proposed. The type strain is RCB-08(T) (=KCTC 13222(T)=CECT 7358(T)).
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- 2009
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181. Paenibacillus pueri sp. nov., isolated from Pu'er tea.
- Author
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Kim BC, Jeong WJ, Kim DY, Oh HW, Kim H, Park DS, Park HM, and Bae KS
- Subjects
- Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial analysis, DNA, Bacterial genetics, Fatty Acids analysis, Fermentation, Genes, rRNA, Gram-Positive Endospore-Forming Rods genetics, Gram-Positive Endospore-Forming Rods isolation & purification, Gram-Positive Endospore-Forming Rods physiology, Molecular Sequence Data, Phenotype, Phylogeny, Quinones analysis, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Species Specificity, Camellia sinensis microbiology, Gram-Positive Endospore-Forming Rods classification, Plant Leaves microbiology
- Abstract
Pu'er tea is a fermented drink made from the leaves of the tea plant, Camellia sinensis. Two novel bacteria, designated strains b09i-3(T) and b13i-1, were isolated during the process of fermentation of this tea. These isolates were Gram-positive, endospore-forming, motile rods that grew at 25-42 degrees C and pH 5.5-10.4. The DNA G+C content was 56.6-58.4 mol%, the predominant isoprenoid quinone was MK-7 and the predominant cellular fatty acid was anteiso-C(15 : 0) (49.0-50 % of the total). Phylogenetic analysis based on 16S rRNA gene sequences showed that strains b09i-3(T) and b13i-1 shared 99.9 % similarity and were affiliated with a cluster within the family Paenibacillaceae. Strains b09i-3(T) and b13i-1 were related most closely to Paenibacillus ginsengihumi DCY16(T) (97 % 16S rRNA gene sequence similarity). Levels of DNA-DNA relatedness between the two novel isolates and P. ginsengihumi DCY16(T) were below 56 %. The phylogenetic and phenotypic characteristics of these novel isolates allowed them to be distinguished clearly from recognized species of the genus Paenibacillus. Based on these data, strains b09i-3(T) and b13i-1 are considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus pueri sp. nov. is proposed. The type species is b09i-3(T) (=KCTC 13223(T)=CECT 7360(T)).
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- 2009
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182. Sphingopyxis ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea.
- Author
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Lee M, Ten LN, Lee HW, Oh HW, Im WT, and Lee ST
- Subjects
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Fatty Acids chemistry, Genes, Bacterial, Genes, rRNA, Genotype, Korea, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S genetics, Sphingomonadaceae chemistry, Sphingomonadaceae isolation & purification, Panax microbiology, Soil Microbiology, Sphingomonadaceae classification, Sphingomonadaceae genetics
- Abstract
A Gram-negative, aerobic, motile, rod-shaped bacterium (strain Gsoil 250(T)) was isolated from soil of a ginseng field in Pocheon province (South Korea) and was characterized using a polyphasic approach in order to determine its taxonomic position. Comparative analysis of 16S rRNA gene sequences showed that strain Gsoil 250(T) belonged to the family Sphingomonadaceae of the class Alphaproteobacteria, and was related to Sphingopyxis macrogoltabida (98.7 %), Sphingopyxis chilensis (98.2 %), Sphingopyxis alaskensis (97.9 %), Sphingopyxis taejonensis (97.9 %) and Sphingopyxis witflariensis (97.8 %). The phylogenetic distance from any other species with validly published names within the genus Sphingopyxis was greater than 3.8 %. The G+C content of the genomic DNA of strain Gsoil 250(T) was 69.2 mol%. Strain Gsoil 250(T) contained Q-10 as the predominant respiratory lipoquinone and C(18 : 1)omega7c and summed feature 4 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 34.6 %) as the major fatty acids. No 3-hydroxy fatty acids were detected. Major polar lipids consisted of sphingoglycolipid, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol and two unknown glycolipids. Phenotypic and chemotaxonomic data supported the affiliation of strain Gsoil 250(T) to the genus Sphingopyxis. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Gsoil 250(T) from the eight recognized Sphingopyxis species. Strain Gsoil 250(T) therefore represents a novel species, for which the name Sphingopyxis ginsengisoli sp. nov. is proposed, with the type strain Gsoil 250(T) (=KCTC 12582(T)=LMG 23390(T)).
- Published
- 2008
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183. Polyamine biosynthesis regulated by StARD expression plays an important role in potato wound periderm formation.
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Kim JH, Kim HS, Lee YH, Kim YS, Oh HW, Joung H, Chae SK, Suh KH, and Jeon JH
- Subjects
- Expressed Sequence Tags, Gene Expression Regulation, Plant, Gene Library, Lipids biosynthesis, Methionine metabolism, Plant Tubers genetics, RNA, Plant genetics, Reverse Transcriptase Polymerase Chain Reaction, Solanum tuberosum genetics, Dioxygenases metabolism, Plant Tubers metabolism, Polyamines metabolism, Solanum tuberosum enzymology
- Abstract
An acireductone dioxygenase (ARD) gene of potatoes was isolated from the expressed sequence tags (ESTs) of potato post-suberization cDNA libraries. The highest expression levels of the StARD gene and the protein appeared 36 h after suberization. An approximate 9-fold increase in ARD activity was detected at 36 h after wounding. Real-time reverse transcription-PCR (RT-PCR) analysis and immunolocalization studies revealed that StARD transcripts increase at the wound surface of potato tubers. The polyamine (PA) contents increased significantly after wounding at the wound surface. The increased PA content and ARD activity may play an important role in wound periderm formation.
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- 2008
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184. Paenibacillus camelliae sp. nov., isolated from fermented leaves of Camellia sinensis.
- Author
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Oh HW, Kim BC, Lee KH, Kim DY, Park DS, Park HM, and Bae KS
- Subjects
- Base Composition, Camellia sinensis metabolism, DNA, Bacterial genetics, DNA, Ribosomal genetics, Gram-Positive Endospore-Forming Rods classification, Gram-Positive Endospore-Forming Rods genetics, Gram-Positive Endospore-Forming Rods metabolism, Molecular Sequence Data, Phylogeny, Plant Leaves metabolism, RNA, Ribosomal, 16S genetics, Camellia sinensis microbiology, Fermentation, Gram-Positive Endospore-Forming Rods isolation & purification, Plant Leaves microbiology
- Abstract
A novel bacterium, strain blls-2(T) was isolated from Pu'er tea. The isolate was Gram-positive, endospore-forming motile rod that grew at 15 approximately 42 degrees C and pH 6.0 approximately 10.2. The DNA G+C content was 48.3 mol%, the predominant isoprenoid quinone was MK-7, and the predominant cellular fatty acid was anteiso-C15:0 (54.2%) followed by C16:0 (15.5%) and iso-C16:0 (8.2%). The polar lipid pattern of blls-2(T) was characterized by the presence of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phy-logenetic analysis based on 16S rRNA gene sequence showed that the strain was affiliated within the Paenibacillaceae. The strain was most closely related to Paenibacillus granivorans A30(T), with a similarity of 97.1%. Based on the phylogenetic and phenotypic characteristics of strain blls-2(T), the isolate is thought to represent a novel taxon in the genus Paenibacillus. The name Paenibacillus camelliae sp. nov. is proposed for the fermented tea isolate; the type strain is blls-2(T) (= KCTC 13220(T)= CECT 7361(T)).
- Published
- 2008
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185. Elevated H(2)O (2) production via overexpression of a chloroplastic Cu/ZnSOD gene of lily (Lilium oriental hybrid 'Marco Polo') triggers ethylene synthesis in transgenic potato.
- Author
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Kim YS, Kim HS, Lee YH, Kim MS, Oh HW, Hahn KW, Joung H, and Jeon JH
- Subjects
- Amino Acid Oxidoreductases metabolism, Cobalt pharmacology, Ethylenes antagonists & inhibitors, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Glycine analogs & derivatives, Glycine pharmacology, Microscopy, Electron, Scanning, Organophosphorus Compounds pharmacology, Phenotype, Plant Stems drug effects, Plant Stems genetics, Plant Stems growth & development, Plant Stems metabolism, Plants, Genetically Modified drug effects, Plants, Genetically Modified genetics, Plants, Genetically Modified growth & development, Silver Nitrate pharmacology, Solanum tuberosum drug effects, Solanum tuberosum genetics, Solanum tuberosum growth & development, Superoxide Dismutase genetics, Ethylenes biosynthesis, Hydrogen Peroxide metabolism, Lilium genetics, Plant Proteins metabolism, Plants, Genetically Modified metabolism, Solanum tuberosum metabolism, Superoxide Dismutase metabolism
- Abstract
Transgenic potato plants (SS2 and SS4) that overexpressed a chloroplastic copper/zinc superoxide dismutase lily gene were utilized as an H(2)O(2)-inducible system in order to study the role of H(2)O(2) as a signaling molecule in the biosynthesis of ethylene. SS2 and SS4 plants grown in vitro under sealed microenvironment (SME) conditions displayed anomalous phenotypes including reduction of stem elongation, radial stem growth, and promotion of root hair formation in the generated root, which were similar to ethylene-induced responses. In addition, SS4 plants showed severe vitrification in developing leaves and elevated ethylene production under SME conditions. After the ethylene action inhibitor AgNO(3), 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) inhibitor CoCl(2), and ACC synthase inhibitor L -aminoethoxyvinylglycine were added to the growth media, the anomalous phenotypes in SS4 plants reverted to their normal phenotype with a concurrent decrease in ethylene production. Northern blot analysis showed that ACO transcripts in SS4 plants were constantly at high levels under normal and SME conditions, indicating that a high level of H(2)O(2) in SS4 plants up-regulates ACO transcripts. Moreover, the direct treatment of H(2)O(2) in potato plants confirmed the elevated expression of the ACO gene. Taken together, these data suggest that the high concentration of H(2)O(2) in transgenic potato plants stimulates ethylene biosynthesis by activating ACO gene expression.
- Published
- 2008
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186. Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12.
- Author
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Oh HW, Heo SY, Kim DY, Park DS, Bae KS, and Park HY
- Subjects
- Actinomycetales physiology, Amino Acid Sequence, Animals, Endo-1,4-beta Xylanases genetics, Enzyme Stability, Gryllidae physiology, Molecular Sequence Data, Sequence Alignment, Substrate Specificity, Actinomycetales enzymology, Actinomycetales genetics, Endo-1,4-beta Xylanases chemistry, Endo-1,4-beta Xylanases metabolism, Gryllidae microbiology, Symbiosis
- Abstract
An exo-symbiotic bacterium capable of hydrolyzing xylan was isolated from the gut of the mole cricket, Gryllotalpa orientalis, and identified as Cellulosimicrobium sp. HY-12. The xylanase (XylA( CspHY-12)) of this organism bound tightly to both DEAE and mono Q resins, and its molecular mass (M(r)) was about 39.0 kDa. The highest xylanase activity was observed at pH 6.0 and 60 degrees C. The enzyme was greatly suppressed by Ca(2+), Cu(2+), Co(2+), and Fe(2+) ions but not by Mg(2+) and Mn(2+). Although XylA( CspHY-12) was capable of hydrolyzing various types of xylosic compounds, it could not decompose carboxymethyl cellulose or xylobiose. The xylA (CspHY-12 ) gene consisted of an 1,188 bp open reading frame that encoded a polypeptide of 395 amino acids with a deduced molecular mass of 42,925 Da. The domain structure of XylA( CspHY-12) was most similar to those of the glycoside hydrolase (GH) family 10 endoxylanases. However its sequence identity with any of the enzymes in this family was below 52%. The results of this study suggest that the XylA( CspHY-12) is a new cellulase-free endo-beta-1,4-xylanase with some properties that are distinct from those of GH family 10.
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- 2008
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187. Ultrastructural analysis of chemical synapses and gap junctions between Drosophila brain neurons in culture.
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Oh HW, Campusano JM, Hilgenberg LG, Sun X, Smith MA, and O'Dowd DK
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- Animals, Animals, Genetically Modified, Animals, Newborn, Cells, Cultured, Drosophila, Drosophila Proteins genetics, Drosophila Proteins metabolism, Gap Junctions metabolism, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Microscopy, Electron methods, Synapses metabolism, Ultrasonography, Brain cytology, Gap Junctions ultrastructure, Neurons ultrastructure, Synapses diagnostic imaging
- Abstract
Dissociated cultures from many species have been important tools for exploring factors that regulate structure and function of central neuronal synapses. We have previously shown that cells harvested from brains of late stage Drosophila pupae can regenerate their processes in vitro. Electrophysiological recordings demonstrate the formation of functional synaptic connections as early as 3 days in vitro (DIV), but no information about synapse structure is available. Here, we report that antibodies against pre-synaptic proteins Synapsin and Bruchpilot result in punctate staining of regenerating neurites. Puncta density increases as neuritic plexuses develop over the first 4 DIV. Electron microscopy reveals that closely apposed neurites can form chemical synapses with both pre- and postsynaptic specializations characteristic of many inter-neuronal synapses in the adult brain. Chemical synapses in culture are restricted to neuritic processes and some neurite pairs form reciprocal synapses. GABAergic synapses have a significantly higher percentage of clear core versus granular vesicles than non-GABA synapses. Gap junction profiles, some adjacent to chemical synapses, suggest that neurons in culture can form purely electrical as well as mixed synapses, as they do in the brain. However, unlike adult brain, gap junctions in culture form between neuronal somata as well as neurites, suggesting soma ensheathing glia, largely absent in culture, regulate gap junction location in vivo. Thus pupal brain cultures, which support formation of interneuronal synapses with structural features similar to synapses in adult brain, are a useful model system for identifying intrinsic and extrinsic regulators of central synapse structure as well as function.
- Published
- 2008
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188. Isolation and functional analysis of a 24-residue linear alpha-helical antimicrobial peptide from Korean blackish cicada, Cryptotympana dubia (Homoptera).
- Author
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Leem JY, Park DS, Suh EY, Hur JH, Oh HW, and Park HY
- Subjects
- Animals, Antimicrobial Cationic Peptides chemistry, Antimicrobial Cationic Peptides isolation & purification, Cell Membrane Permeability, Circular Dichroism, Hemiptera chemistry, Hemolytic Agents analysis, Microscopy, Confocal, Rats, Antimicrobial Cationic Peptides metabolism, Hemiptera metabolism
- Abstract
A new antimicrobial peptide, cryptonin, was isolated and characterized from the adult Korean blackish cicada, Cryptotympana dubia. It consists of 24 amino acid residues and has a molecular weight of 2,704 Da on mass spectroscopy. The predicted alpha-helical structure analysis and increased helix percent in 40% trifloroethanol of cryptonin suggests that it belongs to the typical linear alpha-helix forming peptide. Binding of the biotin-labeled cryptonin at the surface of E. coli cells and increased influx of propidium iodide in E. coli after cryptonin treatment indicates that it kills microbial cells by binding bacterial cell surfaces and disrupting the cell permeability. Cryptonin showed strong antibacterial (MIC 1.56-25 microg/ml) and antifungal (MIC 3.12-50 microg/ml) activities against tested bacteria and fungi including two antibiotic-resistant bacterial strains; methicilin-resistant S. aureus and vancomycin-resistant Enterococci (MIC 25 microg/ml, each).
- Published
- 2007
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189. A culture-based study of the bacterial communities within the guts of nine longicorn beetle species and their exo-enzyme producing properties for degrading xylan and pectin.
- Author
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Park DS, Oh HW, Jeong WJ, Kim H, Park HY, and Bae KS
- Subjects
- Animals, Bacteria classification, Bacteria enzymology, Bacteria genetics, DNA, Ribosomal genetics, Enzymes genetics, Phylogeny, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Bacteria isolation & purification, Coleoptera microbiology, Digestive System microbiology, Pectins metabolism, Xylans metabolism
- Abstract
In this study, bacterial communities within the guts of several longicorn beetles were investigated by a culture-dependent method. A total of 142 bacterial strains were isolated from nine species of longicorn beetle, including adults and larvae. A comparison of their partial 16S rRNA gene sequences showed that most of the bacteria constituting the gut communities can typically be found in soil, plants and the intestines of animals, and approximately 10% were proposed as unreported. Phylogenetic analysis demonstrated that the bacterial species comprised 7 phyla, and approximately half were Gammaproteobacteria. Actinobacteria were the second most populous group (19%), followed by Firmicutes (13%) and Alphaproteobacteria (11%). Betaproteobacteria, Flavobacteria, and Acidobacteria were minor constituents. The taxonomic compositions of the isolates were variable according to the species of longicorn beetle. Particularly, an abundance of Actinobacteria existed in Moechotypa diphysis and Mesosa hirsute, which eat broadleaf trees; however, no Actinobacteria were isolated from Corymbia rubra and Monochamus alternatus, which are needle-leaf eaters. Considerable proportions of xylanase and pectinase producing bacteria in the guts of the longicorn beetles implied that the bacteria may play an important role in the digestion of woody diets. Actinobacteria and Gammaproteobacteria were the dominant xylanase producers in the guts of the beetles.
- Published
- 2007
190. Characterization of an extracellular lipase in Burkholderia sp. HY-10 isolated from a longicorn beetle.
- Author
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Park DS, Oh HW, Heo SY, Jeong WJ, Shin DH, Bae KS, and Park HY
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Burkholderia classification, Burkholderia isolation & purification, Cations pharmacology, Chromatography, Thin Layer, Kinetics, Lipase genetics, Molecular Sequence Data, Molecular Weight, Polymerase Chain Reaction, Thermodynamics, Burkholderia enzymology, Coleoptera microbiology, Lipase isolation & purification, Lipase metabolism
- Abstract
Burkholderia sp. HY-10 isolated from the digestive tracts of the longicorn beetle, Prionus insularis, produced an extracellular lipase with a molecular weight of 33.5 kDa estimated by SDS-PAGE. The lipase was purified from the culture supernatant to near electrophoretic homogenity by a one-step adsorption-desorption procedure using a polypropylene matrix followed by a concentration step. The purified lipase exhibited highest activities at pH 8.5 and 60 degrees . A broad range of lipase substrates, from C4 to C18 rho-nitrophenyl esters, were hydrolyzed efficiently by the lipase. The most efficient substrate was rho-nitrophenyl caproate (C6). A 2485 bp DNA fragment was isolated by PCR amplification and chromosomal walking which encoded two polypeptides of 364 and 346 amino acids, identified as a lipase and a lipase foldase, respectively. The N-terminal amino acid sequence of the purified lipase and nucleotide sequence analysis predicted that the precursor lipase was proteolytically modified through the secretion step and produced a catalytically active 33.5 kDa protein. The deduced amino acid sequence for the lipase shared extensive similarity with those of the lipase family I.2 of lipases from other bacteria. The deduced amino acid sequence contained two Cystein residues forming a disulfide bond in the molecule and three, well-conserved amino acid residues, Ser131, His330, and Asp308, which composed the catalytic triad of the enzyme.
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- 2007
191. Superoxide anion regulates plant growth and tuber development of potato.
- Author
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Kim MS, Kim HS, Kim YS, Baek KH, Oh HW, Hahn KW, Bae RN, Lee IJ, Joung H, and Jeon JH
- Subjects
- Gibberellins genetics, Gibberellins metabolism, Plant Tubers growth & development, Plant Tubers metabolism, Reactive Oxygen Species metabolism, Plant Proteins metabolism, Solanum tuberosum growth & development, Solanum tuberosum metabolism, Superoxide Dismutase metabolism, Superoxides metabolism
- Abstract
A higher concentration of H2O2 was detected in the sense transgenic potato plant (SS4) with the lily chCu,ZnSOD sequence, whereas higher levels of O2(-) was detected in the antisense transgenic plant (SA1) than the WT plant. The elongation growth in SA1 was significantly inhibited by treatment with diphenyleneiodonium, an inhibitor of O2(-) generation, and promoted in the SS4 on treatment with herbicide methyl viologen, a generator of apoplastic O2(-) . Higher concentrations of GAs were detected during plant growth and the early stage of tuberization in SA1. Complete recovery of the above elongation growth and microtuberization pattern in transgenic plants following treatment of GA(3) or an inhibitor of gibberellin synthesis, paclobutrazol, indicate that these changes were mainly caused by active GA levels. In conclusion, a specific ROS (O2(-) ) acts as a signal transducer via GA biosynthetic pathways for the regulation of plant growth and tuber development of potato.
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- 2007
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192. Capsicum annuum CCR4-associated factor CaCAF1 is necessary for plant development and defence response.
- Author
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Sarowar S, Oh HW, Cho HS, Baek KH, Seong ES, Joung YH, Choi GJ, Lee S, and Choi D
- Subjects
- Amino Acid Sequence, Capsicum cytology, Capsicum microbiology, Electrophoresis, Agar Gel, Gene Expression, Gene Expression Profiling, Gene Silencing, Genome, Plant, Solanum lycopersicum cytology, Solanum lycopersicum microbiology, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Phytophthora physiology, Plant Diseases, Plants, Genetically Modified cytology, Regulon, Sequence Analysis, DNA, Xanthomonas axonopodis physiology, Adaptation, Physiological, Capsicum growth & development, Solanum lycopersicum growth & development, Plant Proteins physiology
- Abstract
The CCR4-associated factor 1 (CAF1) protein belongs to the CCR4-NOT complex, which is an evolutionary conserved protein complex and plays an important role in the control of transcription and mRNA decay in yeast and mammals. To investigate the function of CAF1 in plants, we performed gain- and loss-of-function studies by overexpression of the pepper CAF1 (CaCAF1) in tomato and virus-induced gene silencing (VIGS) of the gene in pepper plants. Overexpression of CaCAF1 in tomato resulted in significant growth enhancement, with increasing leaf thickness, and enlarged cell size by more than twofold when compared with the control plants. A transmission electron microscopic analysis revealed that the CaCAF1-transgenic tomato plants had thicker cell walls and cuticle layers than the control plants. In addition to developmental changes, overexpression of CaCAF1 in tomato plants resulted in enhanced resistance against the oomycete pathogen Phytophthora infestans. Additionally, microarray, northern and real-time polymerase chain reaction analyses of CaCAF1-transgenic tomato plants revealed that multiple genes were constitutively upregulated, including genes involved in polyamine biosynthesis, defence reactions and cell-wall organogenesis. In contrast, VIGS of CaCAF1 in pepper plants caused significant growth retardation and enhanced susceptibility to the pepper bacterial spot pathogen Xanthomonas axonopodis pv. vesicatoria. Our results suggest roles for plant CAF1 in normal growth and development, as well as in defence against pathogens.
- Published
- 2007
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193. Biochemical and genetic characterization of arazyme, an extracellular metalloprotease produced from Serratia proteamaculans HY-3.
- Author
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Kwak J, Lee K, Shin DH, Maeng JS, Park DS, Oh HW, Son KH, Bae KS, and Park HY
- Subjects
- Albumins metabolism, Amino Acid Sequence, Animals, Cations, Divalent pharmacology, Coenzymes pharmacology, Collagen metabolism, DNA, Bacterial chemistry, DNA, Bacterial genetics, Edetic Acid pharmacology, Enzyme Stability, Gastrointestinal Tract microbiology, Hydrogen-Ion Concentration, Keratins metabolism, Metalloproteases chemistry, Metalloproteases isolation & purification, Metals pharmacology, Molecular Sequence Data, Molecular Weight, Phenanthrolines pharmacology, Protease Inhibitors pharmacology, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Serratia isolation & purification, Spiders microbiology, Substrate Specificity, Temperature, Metalloproteases genetics, Metalloproteases metabolism, Serratia enzymology
- Abstract
Serratia proteamaculans HY-3 isolated from the digestive tract of a spider produces an extracellular protease named arazyme, with an estimated molecular mass of 51.5 kDa. The purified enzyme was characterized as having high activities at wide pH and temperature ranges. We further characterized biochemical features of the enzymatic reactions under various reaction conditions. The protease efficiently hydrolyzed a broad range of protein substrates including albumin, keratin, and collagen. The dependence of enzymatic activities on the presence of metal ions such as calcium and zinc indicated that the enzyme is a metalloprotease, together with the previous observation that the proteolytic activity of the enzyme was not inhibited by aspartate, cysteine, or serine protease inhibitors, but strongly inhibited by 1,10-phenanthroline and EDTA. The araA gene encoding the exoprotease was isolated as a 5.6 kb BamHl fragment after PCR amplification using degenerate primers and subsequent Southern hybridization. The nucleotide sequence revealed that the deduced amino acid sequences shared extensive similarity with those of the serralysin family of metalloproteases from other enteric bacteria. A gene (inh) encoding a putative protease inhibitor was also identified immediately adjacent to the araA structural gene.
- Published
- 2007
194. Antioxidant and anti-inflammatory activities of N-acetyldopamine dimers from Periostracum Cicadae.
- Author
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Xu MZ, Lee WS, Han JM, Oh HW, Park DS, Tian GR, Jeong TS, and Park HY
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal isolation & purification, Antioxidants isolation & purification, Cell Line, Dimerization, Dopamine isolation & purification, Dopamine pharmacology, Drugs, Chinese Herbal isolation & purification, Free Radical Scavengers, Lipid Peroxidation drug effects, Lipoproteins, LDL metabolism, Macrophages drug effects, Macrophages metabolism, Mice, Oxidation-Reduction drug effects, Plant Extracts pharmacology, Thiobarbituric Acid Reactive Substances, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Antioxidants pharmacology, Dopamine analogs & derivatives, Drugs, Chinese Herbal pharmacology, Plants, Medicinal chemistry
- Abstract
A known N-acetyldopamine dimer, (2R,3S)-2-(3',4'-dihydroxyphenyl)-3-acetylamino-7-(N-acetyl-2''-aminoethyl)-1,4-benzodioxane (1) and a new N-acetyldopamine dimer, (2R,3S)-2-(3',4'-dihydroxyphenyl)-3-acetylamino-7-(N-acetyl-2''-aminoethylene)-1,4-benzodioxane (2) were isolated from the methanolic extracts of Periostracum Cicadae. Compounds 1 and 2 inhibited the Cu2+ -mediated, 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH)-mediated, and 3-morpholinosydnonimine (SIN)-1-mediated LDL oxidation in the thiobarbituric acid-reactive substances (TBARS) assay. The antioxidant activities of 1 and 2 were tested with respect to other parameters, such as lag time of conjugated diene formation, relative electrophoretic mobility (REM), and apoB-100 fragmentation on copper-mediated LDL-oxidation. Compounds 1 and 2 also showed 1,1-diphenyl-2-picrylhydrasyl (DPPH) radical scavenging activity. Compound 2 was more efficient than compound 1 at inhibiting the reactive oxygen species (ROS) generation, nitric oxide (NO) production, and nuclear factor-kappaB (NF-kappaB) activity as well as the expression of pro-inflammatory molecules such as inducible nitric oxide synthase (iNOS), interleukin (IL)-6, tumor necrosis factor (TNF)-alpha, and cyclooxygenase (COX)-2 in LPS-induced RAW264.7 cells.
- Published
- 2006
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195. Formosa agariphila sp. nov., a budding bacterium of the family Flavobacteriaceae isolated from marine environments, and emended description of the genus Formosa.
- Author
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Nedashkovskaya OI, Kim SB, Vancanneyt M, Snauwaert C, Lysenko AM, Rohde M, Frolova GM, Zhukova NV, Mikhailov VV, Bae KS, Oh HW, and Swings J
- Subjects
- Chlorophyta genetics, Flavobacteriaceae isolation & purification, Flavobacteriaceae physiology, Korea, Molecular Sequence Data, Oceans and Seas, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Sequence Homology, Nucleic Acid, Species Specificity, Chlorophyta microbiology, Flavobacteriaceae classification
- Abstract
Two marine, heterotrophic, aerobic, yellow-pigmented, agarolytic bacterial strains that are motile by means of gliding were isolated from the green alga Acrosiphonia sonderi and from sea water. Comparative 16S rRNA gene sequence analysis revealed an affiliation between the strains studied and the genus Formosa, a member of the family Flavobacteriaceae. The level of sequence similarity between strain KMM 3901T and Formosa algae KMM 3553T was 99.1 %. The results of DNA-DNA hybridization experiments and phenotypic analysis indicated that the strains represent a novel species of the genus Formosa, for which the name Formosa agariphila sp. nov. is proposed, with KMM 3901T (= KCTC 12365T = LMG 23005T = DSM 15362T) as the type strain. The description of the genus Formosa is emended with newly obtained data.
- Published
- 2006
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196. Gaetbulimicrobium brevivitae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat of the Yellow Sea in Korea.
- Author
-
Yoon JH, Kang SJ, Jung SY, Oh HW, and Oh TK
- Subjects
- Base Composition, Fatty Acids, Gram-Negative Bacteria chemistry, Gram-Negative Bacteria isolation & purification, Gram-Negative Bacteria physiology, Korea, Molecular Sequence Data, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Sequence Homology, Nucleic Acid, Species Specificity, Vitamin K 2 analogs & derivatives, Gram-Negative Bacteria classification, Seawater, Water Microbiology
- Abstract
A Gram-negative, non-spore-forming and rod-shaped gliding bacterium, designated strain SMK-19T, was isolated from a tidal flat sediment of the Yellow Sea, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain SMK-19T grew optimally at 37 degrees C, in the presence of 2-3 % (w/v) NaCl and at pH 7.0-8.0. It contained MK-6 as the predominant menaquinone, and iso-C(17 : 0) 3-OH and iso-C(15 : 0) as the major fatty acids. Major polar lipids were phosphatidylethanolamine, unidentified phospholipids and an amino-group-containing lipid that is ninhydrin-positive. The DNA G+C content was 36.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain SMK-19T formed a distinct evolutionary lineage within the family Flavobacteriaceae. The 16S rRNA gene sequence of strain SMK-19T exhibited similarity values of <94.4 % to those of other members of the family Flavobacteriaceae. Strain SMK-19T was distinguished from phylogenetically related genera by differences in several phenotypic properties. On the basis of phenotypic, phylogenetic and chemotaxonomic data, SMK-19T (= KCTC 12390T = DSM 17196T) was classified at the type strain of a novel genus and species, Gaetbulimicrobium brevivitae gen. nov., sp. nov.
- Published
- 2006
- Full Text
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