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Context: Endodontic biofilm eradication is achieved by chemo-mechanical disinfection. The search for a safer, nontoxic irrigant led us to a natural product, Ecoenzyme. Aim: This study aims to analyze Ecoenzyme (EE) and explore its antimicrobial and biofilm disrupting activity against a 1-week mature multi-species biofilm. Materials and Methods: Qualitative assessment of the phytochemicals present in EE was conducted. Minimal inhibitory concentration (MIC), minimum bactericidal concentration, and zone of inhibition (ZOI) were recorded. Multi-species biofilm of Streptococcus mutans (MTCC 497), Lactobacillus acidophilus (MTCC 10307), and Enterococcus faecalis (ATCC 29212) was grown and time-kill assay was performed to test biofilm disruption for EE, 3.5% sodium hypochlorite (NaOCl) (control). Student's t-test and one-way ANOVA with post hoc analysis were conducted for ZOI and time-kill assay, respectively. Statistical significance was set at P ≤ 0.05. Results: EE contained secondary metabolites having antibacterial properties. MIC was 25% (S. mutans), 50% (E. faecalis), and >50% (L. acidophilus). EE disrupted ~90% of biofilm species in 5 min of exposure while NaOCl achieved ~99.9% reduction. Further reduction by EE progressed over 20 min after which no viable bacteria in the biofilm was cultivable. Conclusions: Lemon peel Ecoenzyme (EE) is antimicrobial with effective biofilm-disrupting properties on a mature multi-species biofilm. However, its effects were slower than 3.5% sodium hypochlorite. [ABSTRACT FROM AUTHOR]

Objectives: The aim of the present study is to assess the means and methods being suggested by Greek orthodontists to their patients for cleaning their removable devices.Materials and Methods: A questionnaire survey was conducted among a total of 418 Greek orthodontists found from the registry of orthodontists in Greece. The clinicians were asked about the means and methods of daily cleaning they usually advise their patients for removable appliances. Furthermore, the questionnaire included questions related to possible complications of these devices and data about the use and type of applied devices.Results: From 418 Greek orthodontists, 279 filled the questionnaire and participated in this study. All but one reported that they use removable appliances in their practice for patients of almost any age. All orthodontists recommend routine manual toothbrushing, but the majority of them also suggest at the same time other oral hygiene aids complementary to brushing, such as immersion in denture cleaners (70.06%), in disinfecting solutions (12.73%) and in vinegar (36.94%). Some 15% of all the practitioners advise the use of ultrasonic instruments for cleaning removable orthodontic appliances. At last, clinicians have faced complications from the use of removable devices in the oral cavity at a statistically significant percentage. The most usual is trauma of the mucosa, mycosis and hyperplasia.Conclusion: Greek orthodontists use removable orthodontic appliances, active or passive, in their practice. Brushing with a toothbrush and the use of cleaning products for removable prostheses are the preferred methods for cleaning removable orthodontic appliances regardless of the orthodontic specialty programme they had graduated from. [ABSTRACT FROM AUTHOR]

With this 24-months stability, we believe that the gut, circulating (blood), and oral microbiomes of LKD may have limited impact to assess long-term risks of cardiovascular and kidney disease in LKD. Changes in the post-transplantation gut microbiome have been described in kidney transplant recipients (KTR), namely a decrease in overall microbial diversity and an increase in Enterobacteriaceae.[[1]] In addition, gut microbiome may influence tacrolimus dosage, and, in a bidirectional relation, the immunosuppressive therapy and the infection and rejection episodes may alter gut microbiome.[3] In the last decade, several alerts have been raised on the long-term risks of living kidney donors (LKD) regarding kidney and cardiovascular disease.[4] While considering these warnings, additional tools should be explored to help to assess these risks in LKD. [Extracted from the article]

The article titled "Keep your Teeth for a Lifetime" emphasizes that tooth loss is not inevitable with age and that natural teeth are meant to last a person's lifetime. It highlights the importance of taking care of teeth to prevent decay and infection, as well as fighting against tooth decay and gum disease. The article also discusses various factors that can lead to tooth loss, such as extensive cavities, fractures, poor nutrition, smoking, unhealthy habits, genetics, incorrect brushing techniques, teeth grinding, and crooked teeth. It concludes by mentioning the effects of tooth loss, including jawbone deterioration, teeth misalignment, and changes in facial appearance. The article provides tips for maintaining oral health, such as regular dental visits, daily oral hygiene routines, brushing twice a day, using fluoride toothpaste, and flossing daily. [Extracted from the article]

The article discusses how hot summers can compromise oral health. The extreme heat and humidity can lead to dry mouth, which promotes bacterial growth and increases the risk of tooth decay. Changes in diet and nutrition, such as consuming sugary drinks and sweets, can also contribute to tooth decay and gum disease. Inconsistent oral hygiene habits during the summer can result in plaque buildup and chronic inflammation. Additionally, the hot climate and exposure to the sun's UV radiation can increase the risk of oral cancer. The article provides tips for safeguarding oral health, including maintaining a balanced diet, avoiding sugary beverages, practicing good oral hygiene, monitoring medications, and regularly visiting the dentist. [Extracted from the article]

This article emphasizes the importance of oral care for children, highlighting the role that parents play in maintaining good oral hygiene. It suggests that oral care should start as early as six months of age and emphasizes the significance of regular brushing, flossing, and dental visits. The article also discusses the prevention of oral diseases, such as cavities and gum disease, and provides tips for treating common dental injuries. Additionally, it mentions the benefits of fluoride and offers guidance on choosing an appropriate toothbrush for children. Overall, the article emphasizes the importance of establishing positive oral health habits early in life to ensure lifelong oral health. [Extracted from the article]

Introduction: Chronic inflammation caused by dietary obesity has been considered to induce lifestyle-related diseases and functional ingredients with anti-inflammatory effects are attracting attention. Although multiple studies on obesity had proved the anti-inflammatory effects of ingestion of lactic acid bacteria (LAB) and other functional ingredients on adipose tissue, the precise effects on the intestine, especially on the individual intestinal segments have not been made clear. In this study, we elucidated the mechanisms of Lactiplantibacillus plantarum (basonym: Lactobacillus plantarum) OLL2712 in suppressing obesity-induced inflammation using high fat diet (HFD)-fed mice obesity model. Methods: We orally administered heat-treated LAB to HFD-fed mice model, and investigated the inflammatory changes in adipose tissue and intestinal immune cells. We also analyzed gut microbiota, and evaluated the inflammation and permeability of the duodenum, jejunum, ileum and colon; four intestinal segments differing in gut bacteria composition and immune response. Results: After 3-week LAB administration, the gene expression levels of proinflammatory cytokines were downregulated in adipose tissue, colon, and Peyer's patches (PP)-derived F4/80+ cells. The LAB treatment alleviated obesityrelated gut microbiota imbalance. L. plantarum OLL2712 treatment helps maintain intestinal barrier function, especially in the ileum, possibly by preventing ZO-1 and Occludin downregulation. Discussion: Our results suggest that the oral administration of the LAB strain regulated the gut microbiota, suppressed intestinal inflammation, and improved the gut barrier, which could inhibit the products of obesity-induced gut dysbiosis from translocating into the bloodstream and the adipose tissue, through which the LAB finally alleviated the inflammation caused by dietary obesity. Barrier improvement was observed, especially in the ileum, suggesting collaborative modulation of the intestinal immune responses by ingested LAB and microbiota. [ABSTRACT FROM AUTHOR]

Terminalia chebula (TC) is a medicinal plant that exhibits antioxidant, anti-inflammatory, and antibacterial properties and that is widely used in Ayurveda and herbal formulations. However, the skin effects of TC as an oral supplement have not been studied. The objective of this study is to determine if oral TC fruit extract supplementation can modulate the skin's sebum production and reduce the appearance of wrinkles. A prospective double-blind placebo-controlled study was conducted on healthy females aged 25–65. Subjects were supplemented with an oral placebo or Terminalia chebula (250 mg capsule, Synastol TC) capsules twice daily for eight weeks. A facial image collection and analysis system was used to assess the facial appearance of wrinkle severity. Standardized, non-invasive tools were used to measure facial moisture, sebum production, transepidermal water loss, melanin index and erythema index. For those who had a baseline sebum excretion rate >80 ug/cm2, TC supplementation produced a significant decrease in forehead sebum excretion rate compared to the placebo at four weeks (−17 decrease vs. 20% increase, p = 0.07) and at eight weeks (−33% decrease vs. 29% increase, p < 0.01). Cheek erythema decreased by 2.2% at eight weeks, while the placebo treatment increased cheek erythema by 1.5% (p < 0.05). Facial wrinkles decreased by 4.3% in the TC group and increased by 3.9% in the placebo group after eight weeks of supplementation (p < 0.05). TC supplementation reduces facial sebum and improves the appearance of wrinkles. Future studies should consider evaluating oral TC as adjuvant therapy for acne vulgaris. [ABSTRACT FROM AUTHOR]

One strategy in caries prevention is to inhibit the formation of cariogenic biofilms. Attempts are being made to develop oral hygiene products enriched with various antimicrobial agents. One of them is lactoperoxidase—an enzyme that can oxidise (pseudo)halide ions to reactive products with antimicrobial activity. Currently, commercially available products utilise thiocyanate as a substrate; however, several alternatives that are oxidised to products with greater antimicrobial potential have been found. In this study, toxicity against human gingival fibroblasts of the lactoperoxidase system was evaluated using four different (pseudo)halide substrate systems—thiocyanate, iodide, selenocyanate, and a mixture of thiocyanate and iodide. For this purpose, cells were treated with the systems and then apoptosis, cell cycle, intracellular glutathione concentration, and mitochondrial superoxide production were assessed. The results showed that each system, after generating 250 µM of the product, inhibited cell divisions, increased apoptosis, and increased the percentage of dead cells. It was concluded that the mechanism of the observed phenomena was not related to increased superoxide production or the depletion of glutathione concentration. These findings emphasised the need for the further in vitro and in vivo toxicity investigation of the modified lactoperoxidase system to assess its safety and the possibility of use in oral hygiene products. [ABSTRACT FROM AUTHOR]

This meta-analysis intended to assess evidence on the efficacy of locally delivered curcumin/turmeric as an adjunctive to scaling and root planing (SRP), on clinical attachment level (CAL) and probing pocket depth (PPD), compared to SRP alone or in combination with chlorhexidine (CHX). RCTs were identified from PubMed, Cochrane Library, BASE, LIVIVO, Dentistry Oral Sciences Source, MEDLINE Complete, Scopus, ClinicalTrials.gov, and eLibrary, until August 2022. The risk of bias (RoB) was assessed with the Cochrane Risk of Bias tool 2.0. A random-effects meta-analysis was performed by pooling mean differences with 95% confidence intervals. Out of 827 references yielded by the search, 23 trials meeting the eligibility criteria were included. The meta-analysis revealed that SRP and curcumin/turmeric application were statistically significantly different compared to SRP alone for CAL (−0.33 mm; p = 0.03; 95% CI −0.54 to −0.11; I2 = 62.3%), and for PPD (−0.47 mm; p = 0.024; 95% CI −0.88 to −0.06; I2 = 95.5%); however, this difference was considered clinically meaningless. No significant differences were obtained between patients treated with SRP and CHX, compared to SRP and curcumin/turmeric. The RoB assessment revealed numerous inaccuracies, thus raising concerns about previous overestimates of potential treatment effects. [ABSTRACT FROM AUTHOR]

This study evaluated the effects of calcium glycerophosphate (CaGP), with or without fluoride (F), on dual-species biofilms of Streptococcus mutans and Candida albicans. The biofilms were treated three times with 0.125, 0.25, and 0.5% CaGP solutions, with or without 500 ppm F (NaF). Additionally, 500 and 1100 ppm F-solutions and artificial saliva served as controls. After the final treatment, the microbial viability and biofilm structure, metabolic activity, total biomass production, and the composition of the extracellular matrix composition were analyzed. Regardless of the presence of F, 0.25 and 0.5% CaGP promoted a higher biomass production and metabolic activity increase than the controls (p < 0.05). F-free CaGP solutions reduced bacterial cell population significantly more than the 500 ppm F group or the negative control (p < 0.05). All the groups reduced the proteins, and 0.5% CaGP combined with F led to the highest reduction in the carbohydrate and nucleic acids content of the extracellular matrix (p < 0.05). It can be concluded that CaGP alone affected the number of bacterial cells and, when combined with F, reduced its production of biomass, metabolic activity, and the expression of the extracellular matrix components. [ABSTRACT FROM AUTHOR]

Report on dental appointment practices during pediatric visits in the municipality of Pereiro CE, Brazil. Experience report described by nursing and dentistry professionals, from March to September 2022. 136 pediatric visits were held for children ages 0 to 2 years old. Field diaries were used as the data collection method. It was found that: only 13% cleaned the child's mouth in the absence of teeth; initiating brushing with a toothbrush when the first tooth erupted was done by only 18%; using fluoride toothpaste on the brush was done by only 8% and all mothers were unaware of the information that increased fruit intake prevents cavities. Finally, implementing pediatric dentistry in the unit's routine. And the "cold dental chair" is transformed into smiles, thus considering this work innovative and relevant so that children can go from the crib to the dental chair safely, with support and free of fear. [ABSTRACT FROM AUTHOR]

Considering the unfavorable implications of health falsehoods and the lack of dental research into information disorder, this study aimed to identify and characterize online oral health misinformation. A total of 410 websites published in English were retrieved using Google Advanced Search and screened by two independent investigators to compile falsehoods through thematic content analysis. Afterward, 318 pieces of misinformation were consensually divided into four groups concerning their informational interest (G1), financial, psychological, and social interests produced/disseminated by non-dental professionals (G2) or by dental professionals (G3), and political interests (G4). Social media (Facebook and Instagram) and fact-checking tool (Snopes) were also screened to determine the spread of falsehoods by identifying corresponding posts and warnings. As a result, misinformation was mainly associated with gum diseases (12.0%), root canal treatment (11.6%), toothache (10.4%), fluoride (10.4%), and dental caries (9.8%), with a special highlight on recommendations for the usage of natural products, toxicity concerns, and anti-fluoridation propaganda. Additionally, most misinformation was allocated in G3 (41.9%), which presented a statistically higher frequency of financial interests than G4. Finally, falsehoods were considerably identified on Facebook (62.9%) and Instagram (49.4%), especially G3 and G4. Nevertheless, Snopes has debunked only 5.9% of these content items. Therefore, misinformation was predominantly produced or disseminated by dental professionals mainly motivated by financial interests and usually linked to alternative/natural treatments. Although these items were shared on social media, fact-checking agencies seemed to have limited knowledge about their dissemination. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study was to test heat-treatment methods for improving the cytocompatibility of dental 3D printable photopolymer resins. Methods: Nextdent C&B resin and a digital light processing 3D printer were used to print all specimens, which were divided into seven groups as follows: 1-month storage at controlled room temperature, 20 to 25 °C (RT), 24-hour storage at RT, 24-hour storage in RT water, 1-min immersion in 80 °C water, 1-min immersion in 100 °C water, 5-min immersion in 100 °C water, and autoclaving. Cell viability tests, cytotoxicity tests, and confocal laser scanning microscopy were performed to analyze the cytocompatibility of the 3D-printed resin. Fourier-transform infrared spectroscopy was performed after heat-treatment to determine the degree of conversion (DC). Results: Immersing printed resin samples in 100 °C water for 1 or 5 min after the curing process was an effective method for increasing cytocompatibility by inducing the preleaching of toxic substances such as unpolymerized monomers, photoinitiators, and additives. Moreover, the DC can be increased by additional polymerization without affecting the mechanical properties of the material. Conclusions: Immersing the printed photosensitive dental resins in 100 °C water for 5 min is a suitable method for increasing cytocompatibility and the DC. [ABSTRACT FROM AUTHOR]

Objective: To green synthesise gold nanoparticles using curcumin and to analyse its antioxidant, anti-inflammatory, and antimicrobial activity among oral pathogens. Material and Methods: Biosynthesised Curcumin Gold nanoparticles (CuAuNP) were evaluated by UV-visible spectrophotometer (UV-Vis), Transmission Electron Microscopy (TEM), and evaluation of antioxidant, anti-inflammatory and antibacterial activity against oral pathogens. Results: Synthesized CuAuNP were characterized using UV-visible spectrophotometry and showed peak absorption at 530nm. CuAuNp showed a 90.3% maximum scavenging ability of DPPH at a concentration of 50 μg/mL. CuAuNP exhibited 79.6 % of the highest anti-inflammatory activity at 50μg/mL than the standard drug diclofenac. TEM image clearly showed uniformly dispersed spherical-shaped gold nanoparticles with a size of about 20 nm. The biosynthesized nanoparticle was tested for its antimicrobial effect, and it showed a potent effect against S. aureus, E. faecalis, and C. albicans at 100µg/ mL. Enterococcus faecalis has a maximum zone of inhibition of 14 mm at 100µg/ mL of CuAuNp. Among gram-positive bacteria, a maximum zone of inhibition of 12 mm at 100µg/ mL was seen in S. aureus compared to S mutans. Candida albicans showed a maximum zone of inhibition of 18 mm at 25 μg/mL of CuAuNp. Conclusion: Curcumin-mediated gold nanoparticles with 20 nm size were effective and had strong antioxidant and anti-inflammatory activity at 50µg/ mL, antimicrobial action inhibiting microbes at 100µg/mL concentration that can be used in treating various Oral mucosal lesions. [ABSTRACT FROM AUTHOR]

Eating habits describe the types and quantities of food that an individual or a group of individuals regularly consumes, as well as how often they drink. Healthy dietary practises have an impact on dental health. The future embryo's development, including the growth of the maxilla, the formation of the skull and face, and dental organogenesis, is impacted by a lack of vitamins and minerals during the preconception period. Understanding how nutrition and eating habits affect both children's and adults' caries rates is necessary to improve oral health. Lack of vitamin A, C, E, and folate all has a detrimental effect on periodontal health. Throughout the early phases of embryonic development, folate is crucial. Oral cancer is most common in people who smoke, chew tobacco, or consume alcohol. Changing one's nutrition can have a significant impact on one's health throughout one's life, both positively and negatively. Food consumption must be improved in order to minimise the incidence of oral and pharyngeal cancer. The primary sources of vitamins and fibre are fruits, vegetables, and grains, thus a daily meal should have an appropriate quantity of each. By giving folate during the first trimester of pregnancy and in the months leading up to conception, the likelihood of nervous system abnormalities is reduced. Chewing gum and other xylitol-containing products have significantly decreased dental cavities, and they provide tempting alternatives for people who are most at risk. The aim of this study is to review the evidence suggesting link between diet, nutrition, and oral health and to provide dietary advice for the prevention of oral diseases. [ABSTRACT FROM AUTHOR]

Aims and Objectives: The use of medicinal herbs to prevent gingival and periodontal diseases has become increasingly popular due to their anti-inflammatory and antioxidant properties. This systematic review aims to provide the current literature to validate the traditional use of medicinal herbs in the management of gingival and periodontal diseases. Materials and Methods: An online literature search was conducted to identify research papers published from 2010 to 2022 in three major scientific databases, PubMed, Scopus, and Web of Science, in June 2022. Original research studies, case reports, and systematic reviews on medicinal plants' application in oral health care were selected to be included in this systematic review. Only high-quality articles identified in the quality assessment were included for evidence synthesis. Results: Initial keyword research yielded 726 free-text articles published between 2010 and 2022. Of these, 14 articles (8 research papers and 6 reviews) were included for evidence synthesis. The review's findings indicate that the antibacterial property of medicinal plants is due to their alkaline nature and prevents plaque and calculus formation by maintaining acid–alkali balance in saliva. Various parts of medicinal plants help maintain periodontal health. Glycyrrhiza glabra, Ficus religiosa, and Plantago major effectively inhibit primary plaque colonizers and periodontal pathogens. Medicago sativa, Aloe barbadensis Miller, and Trifolium pratense have excellent applications in treating periodontal diseases. Mangifera indica, Pongamia pinnata, the husk of Cocos nucifera, the root of G. glabra and Curcuma longa, leaves of Psidium guajava and Azadirachta indica, fruits of Citrus medica and Punica granatum, Ocimum Moringa oleifera extract, and pomegranate peel extract can serve as a promising alternative in managing chronic gingivitis. Conclusion: The anti-inflammatory, antioxidant, antibacterial, and astringent action of extracts obtained from various parts of medicinal plants make them effective in reducing gingival and periodontal diseases. Herbal medicine may be a viable alternative to contemporary pharmaceuticals as an adjuvant to scaling and root planning procedures. [ABSTRACT FROM AUTHOR]

Background: In Alzheimer's disease (AD), amyloid-β 1–42 (Aβ42) neurotoxicity stems mostly from its soluble oligomeric aggregates. Studies of such aggregates have been hampered by the lack of oligomer-specific research tools and their intrinsic instability and heterogeneity. Here, we developed a monoclonal antibody with a unique oligomer-specific binding profile (ALZ-201) using oligomer-stabilising technology. Subsequently, we assessed the etiological relevance of the Aβ targeted by ALZ-201 on physiologically derived, toxic Aβ using extracts from post-mortem brains of AD patients and controls in primary mouse neuron cultures. Methods: Mice were immunised with stable oligomers derived from the Aβ42 peptide with A21C/A30C mutations (AβCC), and ALZ-201 was developed using hybridoma technology. Specificity for the oligomeric form of the Aβ42CC antigen and Aβ42 was confirmed using ELISA, and non-reactivity against plaques by immunohistochemistry (IHC). The antibody's potential for cross-protective activity against pathological Aβ was evaluated in brain tissue samples from 10 individuals confirmed as AD (n=7) and non-AD (n=3) with IHC staining for Aβ and phosphorylated tau (p-Tau) aggregates. Brain extracts were prepared and immunodepleted using the positive control 4G8 antibody, ALZ-201 or an isotype control to ALZ-201. Fractions were biochemically characterised, and toxicity assays were performed in primary mouse neuronal cultures using automated high-content microscopy. Results: AD brain extracts proved to be more toxic than controls as demonstrated by neuronal loss and morphological determinants (e.g. synapse density and measures of neurite complexity). Immunodepletion using 4G8 reduced Aβ levels in both AD and control samples compared to ALZ-201 or the isotype control, which showed no significant difference. Importantly, despite the differential effect on the total Aβ content, the neuroprotective effects of 4G8 and ALZ-201 immunodepletion were similar, whereas the isotype control showed no effect. Conclusions: ALZ-201 depletes a toxic species in post-mortem AD brain extracts causing a positive physiological and protective impact on the integrity and morphology of mouse neurons. Its unique specificity indicates that a low-abundant, soluble Aβ42 oligomer may account for much of the neurotoxicity in AD. This critical attribute identifies the potential of ALZ-201 as a novel drug candidate for achieving a true, clinical therapeutic effect in AD. [ABSTRACT FROM AUTHOR]

Objective. The aim of this study was to investigate if desquamated oral epithelial cells (DOECs) express the epidermal growth factor (EGF) and if these cells thereby may contribute to salivary EGF contents. Background. DOECs have recently been shown to harbor the antimicrobial peptide LL-37, proposing that they may also store other biologically important salivary peptides/proteins. The EGF peptide is a growth factor which plays a critical role to maintain epithelial integrity and promote epithelial healing. The EGF is produced by salivary glands, but it is not known whether DOECs contain the EGF and thereby contribute to salivary EGF levels. Materials and Methods. DOECs were isolated from unstimulated whole saliva collected from four healthy volunteers. EGF protein expression was determined in cell lysates by dot blot and ELISA. Cellular distribution of cytokeratin, the proliferation marker Ki67, and EGF immunoreactivity were assessed by immunocytochemistry. EGF gene expression was investigated by qPCR. Expression of EGF transcript and protein in DOECs was compared to that in the human cultured keratinocyte cell line (HaCaT) cells. Results. EGF protein expression was detected in DOEC cell lysates by both dot blot and ELISA. Strong cytoplasmic EGF immunoreactivity was observed in DOECs, although some cells showed only a weak immunoreactive signal for EGF. Moreover, DOECs, besides containing EGF protein, also expressed transcript for EGF. Interestingly, ELISA analysis revealed that EGF protein contents were higher in DOECs than in HaCaT cells. ELISA analysis also disclosed that EGF concentration was about 10 times higher in whole saliva compared to DOECs. EGF transcript expression was about 50% lower in HaCaT cells stimulated with high (10%) compared to low (0.1%) concentration of fetal bovine serum, representing growth-stimulated and growth-restricted conditions, respectively, implying that growth-stimulus exerts negative feedback on EGF gene activity in HaCaT cells. Conclusion. Here, we show for the first time that DOECs express the EGF, arguing that these cells contribute to salivary EGF contents and hence may play a role in gingival epithelial repair and wound healing. [ABSTRACT FROM AUTHOR]

Natural antimicrobial products have been historically used for the prevention and control of biofilm-dependent oral diseases. We determined the physicochemical characteristics of Origanum vulgare L. essential oil (OEO) and carvacrol (CAR), and their in vitro antimicrobial activity against cariogenic bacteria. In silico analysis was further carried out to examine the pharmacokinetics of CAR. The antimicrobial activity of OEO and CAR was determined through agar diffusion test, biofilm assays, and the checkboard test. Data were analyzed by Tukey's post hoc test. OEO showed inhibitory activity on bacterial growth, which was enhanced with the addition of CAR and greater than that of CAR alone. In silico analysis indicated good theoretical bioavailability of CAR. CAR showed effective physicochemical characteristics as an antimicrobial drug due to its favorable theoretical absorption and distribution kinetics. Collectively, our findings suggest that OEO and CAR warrant further investigations as promising natural products for controlling cariogenic biofilms. [ABSTRACT FROM AUTHOR]

This work is a review of the results of some studies conducted around the world on the essential oil of Mentha spicata. Thus, chemical analysis by gas chromatography-mass spectrometry (GC-MS) have identified several compounds in the oil. Among the main ones, we cited carvone, limonene, germacrene D, β-caryophyllene, menthone and 1,8-cineole. Indeed, the evaluation of the antioxidant activity of the essential oil by the DPPH test revealed a difference in the results that is probably due to the diversity of the chemical composition, factors and according to the region. As well as, the ABTS test results revealed that the essential oil of Mentha spicata possessed reasonable antioxidant activity. This could be attributed to the presence of a large amount of carvone. In addition, the evaluation of the antimicrobial activity of the essential oil by the technique of aromatogram and the determination of minimum inhibitory concentration (MIC) showed that it possessed a great inhibitory power on Gram positive bacteria like Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Bacillus cereus, and Streptococcus mutans, and Gram negative bacteria like Pseudomonas aeruginosa, Pasturella multocida, Escherichia coli, and Salmonella. As well as on fungi such as Mucor mucedo, Rhizopus solani, Aspergillus niger, Aspergillus flavus, and Alternaria alternate. In another way, the results of cytotoxic activity using MTT assay showed that Mentha spicata essential oil had a potent cytotoxic action on KB and HeLa cells. [ABSTRACT FROM AUTHOR]

Algae are effective predecessors of nutrient foods and preventive drugs, gaining global attraction in recent years. It exhibits potent antiviral, antibacterial, antifungal, anti-inflammatory, antioxidant, anti-glycemic, and cholesterol-lowering properties due to their richness in highly valuable secondary metabolites. Nevertheless, algae produce valuable bioproducts, its application in dentistry is in its primitive stage. This review focuses on the emergence and emerging role of micro/macroalgae as a natural source of therapeutic, preventive, and biocompatible agents in dentistry. Several studies unveiled that Cyanobacteria, Spirulina , and Chlorella species offer high oral antibacterial and antifungal properties compared to gold standard agents. The characteristic of algae to scavenge superoxide and hydroxyl free radicals, fabricate them as an anti-oxidative and anti-cancer agent. Either alone or by synergism with pinnacle therapies they are found to produce promising curative actions against periodontitis by embattling proinflammatory cytokines. Technologies extend the functions of microalgae as a detoxifying agent, potent drug delivery system, and adjunct regenerative material in chronic periodontitis. Its application as thickening, binding, anticariogenic agent in toothpaste, antibacterial agent in mouthwash, and biocompatible agent in dental impression materials remains very primitive. Low-cost and eco-friendly technologies are needed for the production of oral hygiene products using algal biomass. • Potential applications of algal biomass in dentistry. • Dental health care products from algal extracts. • Synthesis of biocompatible material as a thickening, binding, and gel-forming agents from algae. • Algae in oral care treatments like anti-inflammatory, detoxifying and anti-microbial agents. [ABSTRACT FROM AUTHOR]

Background: Studies focusing on the relationship between periodontitis and systemic diseases have suggested a possible association between these two chronic and inflammatory disorders. We aimed to comparatively investigate the salivary oxidative status, biomarker levels, clinical findings, and the microbial load on subgingival biofilm samples in psoriasis patients and controls. Methods: Forty participants were allocated into four groups as follows: (1) systemically and periodontally healthy (C group); (2) systemically healthy with periodontitis (P group); (3) psoriasis (Ps) and periodontally healthy (Ps‐C group); and (4) Ps with periodontitis (Ps‐P group). Subgingival biofilm samples were obtained to detect the periodontopathogenic agents by Real‐time PCR (qPCR). The total antioxidant status (TAS) (mmol/l), total oxidant status (TOS) (μmol/l), and arylesterase (ARE) activity (U/L) were analyzed using saliva samples. Results: The level of TOS and oxidative stress index (OSI) were significantly higher in patients with Ps‐P and P compared to controls (P = 0.001, and P ˂ 0.001, respectively). ARE levels were higher in controls compared to Ps and P (P ˂ 0.001). The prevalences of bacteria detected in subgingival biofilm samples were similar between all groups (P > 0.05). Conclusions: This study reported that psoriasis may amplify TOS and OSI, and the co‐existence of psoriasis and periodontitis may aggravate oxidative stress. [ABSTRACT FROM AUTHOR]

To improve our understanding of the molecular mechanisms underlaying seed priming, RNA transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed priming was performed by submergence in 1% silicic acid for 24h at 4°C, followed by rinsing with sterilised water and desiccation to original moisture content. Silene sendtneri is a species with no sequenced genome and annotation of de novo assembly of transcriptome was done against several species. Gene ontology (GO) analysis indicated that genes related to heavy metal transporters and heat shock proteins are differentially expressed after priming with silicic acid. Within these gene categories, genes such as heavy metal-associated isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30- like isoform X1 (log2fold 5.98) were upregulated. [ABSTRACT FROM AUTHOR]

Introduction: Despite improvements in dentin bonding, the bond will be demolished over time and decreased by water and matrix metalloproteinase (MMP). This study aimed to evaluate the effects of the combination of different methods of MMP inhibition and detect the possible synergic effect on strength and bond durability. Methods: Thirty human third molar teeth without any cracks or decay were used in this study. The occlusal surface of teeth were removed, and dentine surfaces were flattened with silicon carbide plates. After preparing for restoration, teeth were divided into 4 groups, including 1- the control group, 2- the chlorhexidine (CHX) 2% group, 3- the green tea (GT) extract 15%+rinse+CHX 2% in 30 sec, and 4- GT extract15%+rinse+CHX 2% in 60 sec for the application time of GT. Composite samples were divided into two groups, and microtensile bond strength was evaluated immediately and 3 months after storage in water. The fracture type of samples was then evaluated using a stereomicroscope. Results: The immediate bond strength had no significant difference in experimental groups. The mean bond strength for GT+CHX in 30 and 60 sec after a 3-month storage had a significant difference with the 3- month storage control group, however, not significant with the immediate control group. Conclusion: The use of GT extract with CHX led to bond durability after a 3- month storage. Further studies are required for investing the in vivo effect of this combination. [ABSTRACT FROM AUTHOR]

• Online SESI-HRMS aids real-time EBC but lacks separation for compound differentiation. • EBC analyzed using GC–MS and LC-MS can supplement SESI-HRMS data effectively. • SESI-HRMS shows higher sensitivity for some compounds, needing GC–MS and LC-MS annotation. • Optimizing fragmentation techniques is crucial for comprehensive analysis in this field. Secondary electrospray ionization coupled to high-resolution mass spectrometry (SESI-HRMS) is a powerful method for the analysis of exhaled breath in real time. However, feature annotation is challenging due to the flow-injection nature of the technique. To evaluate alternative methods for enhancing feature annotation, a study was conducted where the exhaled breath of sixteen subjects was condensed and analyzed using dynamic headspace vacuum in-trap extraction gas chromatography-mass spectrometry (DHS-V-ITEX-GC–MS) and liquid chromatography coupled to mass spectrometry (LC-MS) using polar and reverse-phase conditions along with a data-independent MS2-acquisition method based on multiple injections. The annotation results obtained from these methods were compared to those from SESI-HRMS. The use of these techniques on breath condensate is unprecedented. The GC–MS method primarily detected compounds of exogenous origin, particularly additives in oral hygiene products like menthol. On the other hand, LC-MS detected a vast number of features, especially with the utilized data-independent acquisition method. Chemical classes to these features were assigned in-silico. In positive ion mode, mostly amino acids and amines were detected, while the largest group in negative ion mode consisted of carboxylic acids. Approximately 25% and 5% of SESI features had a corresponding match with LC-MS and GC–MS. While both GC–MS and LC-MS methods partially overlapped with the SESI features, there was limited overlap of both in the mass-to-charge range from 150 to 200. In conclusion, both GC–MS and LC-MS analysis of breath condensate can serve as supplementary tools for annotating features obtained from SESI-MS. However, to increase confidence in the annotation results, combining these methods with additional on-line fragmentation techniques is recommended. This study presents a comparison between features detected in exhaled breath using SESI-HRMS and those identified in breath condensate. The breath condensate analysis involved the use of a headspace-sampling GC–MS method and a highly sensitive LC-MS2 acquisition approach. Approximately one-fourth of the features detected online exhibited corresponding matches in either the GC–MS or LC-MS analysis. [Display omitted] [ABSTRACT FROM AUTHOR]