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Abstract BRCA1 (Breast Cancer 1, early onset) is linked to breast and ovarian cancer predisposition. Still, the risks conferred by a significant portion of BRCA1 variants identified in the population remains unknown. Most of these variants of uncertain significance are missense alterations. However, the functional implications of small in-frame deletions and/or insertions (indels) are also difficult to predict. Our group has previously evaluated the functional impact of 347 missense variants using an extensively validated transcriptional activity assay. Here we show a systematic assessment of 30 naturally occurring in-frame indels located at the C-terminal region of BRCA1. We identified positions sensitive and tolerant to alterations, expanding the knowledge of structural determinants of BRCA1 function. We further designed and assessed the impact of four single codon deletions in the tBRCT linker region and six nonsense variants at the C-terminus end of BRCA1. Amino acid substitutions, deletions or insertions in the disordered region do not significantly impact activity and are not likely to constitute pathogenic alleles. On the other hand, a sizeable fraction of in-frame indels at the BRCT domain significantly impact function. We then use a Bayesian integrative statistical model to derive the probability of pathogenicity for each variant. Our data highlights the importance of assessing the impact of small in-frame indels in BRCA1 to improve risk assessment and clinical decisions for carriers.

Typhoidal salmonellosis is a global public health problem occurring in developing endemic regions. In Brazil, cases are mostly registered in the North and Northeast regions. Molecular characterization of the strains is important to understand the epidemiology of disease infections and to design control strategies. The present study retrospectively evaluates the genotyping features of sporadic and outbreak-related Salmonella Typhi isolates from the Brazilian North region. Bacterial isolates were recovered from blood and a rectal swab of patients in the states of Acre and Pará, Brazilian North region, in the period of 1995 to 2013, and were submitted to genotyping by applying Multilocus sequence typing (MLST) and Pulsed Field Gel Electrophoresis (PFGE) reference methods. MLST genotyping revealed the presence of epidemic clones ST1 and ST2, and 20 pulsotypes were identified by PFGE, including four distinct clusters (A–D), and six subclusters (A1–D1) with indistinguishable strains in different periods and locations. To conclude, the obtained data demonstrates the temporal stability, adaptation, and transmission of outbreak-related and sporadic S. Typhi strains over time, contributing to the transmission chain in the region.

Browsing is a prevalent activity on the World Wide Web, and users usually demonstrate significant expectations for expeditious information retrieval and seamless transactions. This article presents a comprehensive performance evaluation of the most frequently accessed webpages in recent years using Data Envelopment Analysis (DEA) adapted to the context (inverse DEA), comparing their performance under two distinct communication protocols: TCP/IP and QUIC. To assess performance disparities, parametric and non-parametric hypothesis tests are employed to investigate the appropriateness of each website’s communication protocols. We provide data on the inputs, outputs, and efficiency scores for 82 out of the world’s top 100 most-accessed websites, describing how experiments and analyses were conducted. The evaluation yields quantitative metrics pertaining to the technical efficiency of the websites and efficient benchmarks for best practices. Nine websites are considered efficient from the point of view of at least one of the communication protocols. Considering TCP/IP, about 80.5% of all units (66 webpages) need to reduce more than 50% of their page load time to be competitive, while this number is 28.05% (23 webpages), considering QUIC communication protocol. In addition, results suggest that TCP/IP protocol has an unfavorable effect on the overall distribution of inefficiencies.

Objetivo: analisar os erros que ocorrem no preparo de medicamentos por sondas enterais em terapia intensiva. Método: estudo observacional, com desenho transversal, norteado pelas recomendações do Strengthening the Reporting of Observational Studies in Epidemiology (STROBE), realizado em quatro unidades de terapia intensiva de um hospital privado da cidade do Rio de Janeiro. Para avaliar o preparo de medicamentos na forma farmacêutica sólida as variáveis foram: trituração e diluição. Para os medicamentos líquidos somente a variável diluição. A unidade de medida utilizada para o cálculo amostral foi a dose preparada do medicamento, sendo analisadas 720 doses de medicamentos prescritos por sondas. Foram observados 80 técnicos de enfermagem no momento de preparo dos medicamentos garantindo-se que o profissional fosse observado no manejo de no mínimo nove doses de medicamentos. Resultados: Foi identificado erro no preparo de 480 doses (66,66%). Os comprimidos revestidos e cápsulas de gelatina dura foram triturados indevidamente em 100% das doses analisadas e 54% dos comprimidos simples tiveram tritura insuficiente. A diluição insuficiente ocorreu em 100% das doses de xaropes. A tritura insuficiente para comprimidos simples ocorreu em 310 doses (54%). A diluição insuficiente ocorreu em 100% das doses de xaropes. O erro prevalente foi o de tritura para os medicamentos sólidos. Conclusões: o preparo de medicamentos apresentou uma frequência alta de erros, o que pode levar a perdas na biodisponibilidade do fármaco e comprometer as boas práticas da terapia medicamentosa por sonda enteral.

Este artigo objetiva verificar como produtos capilares da marca Pantene são representados no discurso midiático da publicidade, visando a impulsionar a marca e o consumo. Numa abordagem de cunho qualitativo-interpretativo, analisam-se duas peças publicitárias da Pantene, extraídas do ambiente on-line. A análise ancora-se nas categorias analíticas propostas pelas Gramática Sistêmico-Funcional (GSF), fundamentada em Halliday e Matthiessen (2014), e pela Gramática do Design Visual (GDV), proposta por Kress e van Leeuwen (2021). Conclui-se que a produção de sentidos emerge da interação de vários modos semióticos, dos quais, cada um é responsável por uma tarefa comunicativa, mas são interdependentes entre si. Os resultados apontam para construções multissignificativas, marcadas por processos materiais, mentais e relacionais e pela escolha das funções discursivas de declaração, pergunta e comando, associados às semioses visuais, construindo representações de status, glamour, patriotismo, sedução, maciez, brilho e beleza.

Antecedentes: Las farmacodermias graves se asocian con alta morbilidad y mortalidad. La insuficiencia renal crónica relacionada con diálisis es uno de los principales factores asociados con mortalidad. Reporte de caso: Paciente pediátrico masculino de 2 años, con antecedente de insuficiencia renal crónica (en tratamiento con diálisis peritoneal) y síndrome de Lennox-Gastaut, que ingresó al hospital por peritonitis aguda y evolucion a síndrome de Stevens-Johnson-necrólisis epidérmica tóxica, debido a la administración de fenitoína. Recibió tratamiento con inmunoglobulina y corticosteroide sistémico sin mejoría; se agregó choque séptico y posteriormente falleció. Conclusiones: La escala ABCD-10 versus SCORTEN es más efectiva para predecir la mortalidad por antecedente de diálisis. En niños con farmacodermias graves no existen escalas predictoras de mortalidad validadas. Las futuras iniciativas deben buscar factores de riesgo de mortalidad en niños con farmacodermias graves para la creación de una escala predictora de mortalidad. Palabras clave: Síndrome Stevens-Johnson; necrólisis epidérmica tóxica; diálisis; mortalidad; escala ABCD-10; niños.

Abstract The objective of this work was to develop a method to create and validate CRISPR-Cas systems and different gRNAs in soybean (Glycine max) embryos. Two model genes were used for simple mutation with one gRNA or partial gene deletion with two guides. The gRNAs were inserted into the CRISPR transformation vectors by a type IIS restriction enzyme or by subcloning and inserting the promoter + gRNA2 in the final transformation vector using the classic restriction enzyme cloning method. The vectors were successfully constructed for one and two gRNAs. Agrobacterium-mediated transient transformation in soybean was carried out to test the quality of gRNAs and of the system itself (expression cassette). Simple mutation and gene deletion were detected in the embryos transformed after DNA enrichment by enzyme digestion followed by polymerase chain reaction and sequencing, which indicates that the CRISPR-Cas system and guides were working. This protocol can be used to accelerate CRISPR-based genome editing strategies for genetic transformation in soybean.

Entende-se que o conceito de cidade-dormitório, há muito presente nas discussões sobre São Gonçalo-RJ, provoca uma ausência: as várias possibilidades de ver e representar a cidade se ofuscam sob a onipresença desse conceito. Procuramos tratar das implicações da cristalização de certas representações que se conformam em torno dele. Entende-se que, quando deixa de ser mediação entre o vivido e o concebido, o conceito pode se cristalizar e bloquear o entendimento. Neste artigo, recuperamos alguns usos do conceito cidade-dormitório na Região Metropolitana do Rio de Janeiro confrontando-os com dados da realidade atual do município. Também discutimos implicações simbólicas e materiais devidas à cristalização dessas representações, acompanhadas de seu papel na produção do espaço da cidade. Como resultado de pesquisa, concluímos que é preciso fazer emergir um entendimento que ultrapasse a primazia do centro metropolitano; há que compreender as representações para deslindar seus nós, observá-las para ver além delas.

Resumo Objetivo Analisar os desafios para o exercício da advocacia em saúde à criança hospitalizada durante a pandemia COVID-19. Métodos Estudo qualitativo descritivo-exploratório on-line. Participaram 28 profissionais de enfermagem matriculados na disciplina Enfermagem na Atenção à Saúde da Criança e do Adolescente em um Programa de pós-graduação de uma universidade federal do nordeste brasileiro. A coleta de dados ocorreu em junho de 2021 através de roda de conversa e entrevista coletiva. Como instrumentos utilizou-se: o formulário do google forms e roteiro semiestruturado. O estudo foi aprovado pelo Comitê de Ética em Pesquisa. Como método de análise, foi empregada a Análise Textual Discursiva (ATD). Para a organização dos dados, utilizou-se o software Atlas.ti 8.4.15 (Qualitative Research and Solutions). Resultados Emergiram duas categorias: 1) Impactos da pandemia para assistência e advocacia pediátrica, constatou-se o isolamento infantil e um cenário de atenção à saúde onde a criança foi colocada em segundo plano. 2) Barreiras existentes que se agravaram com a crise sanitária, identificou-se: sobrecarga de trabalho, precarização da estrutura e dificuldade nas condições de trabalho, que gerou violações nos direitos infantis e agravou o panorama de dificuldades na oferta de serviços pediátricos. Conclusão Os desafios para o exercício da advocacia em saúde à criança hospitalizada durante a pandemia, evidenciados pelos impactos e barreiras para a assistência, ampliaram o trabalho das equipes de saúde tornando o exercício da advocacia no cuidado pediátrico ainda mais dificultoso. Cabe repensar e ajustar políticas de acesso e atendimento após a pandemia para assegurar que o cuidado infantil não seja restringido.

ABSTRACT Objective: to evaluate the association between parental supervision and sexual behaviors among Brazilian adolescents. Methods: Cross-sectional study with data from 102,072 adolescents who responded to the National Adolescent School-based Health Survey. We estimated the prevalence of sexual behaviors (initiation, use of condoms, contraception, and number of partners). Parental supervision was evaluated using a score considering five indicators. We calculated prevalence ratios (PR) adjusted by age and sex in order to estimate the association between parental supervision score and sexual behaviors of adolescents. Results: Prevalence of risky sexual behavior for adolescents with minimum and maximum parental supervision were: sexual initiation (min.: 58.0%; max.: 20.1%), condom use in the last sexual intercourse (min.: 50.9%; max.: 80.2%), use of contraceptives (min.: 40.8; max.: 49.1%), and mean number of partners (min.: 3.25; max.: 2.88). Parental supervision was greater among girls. Those with higher supervision scores had higher prevalence of condom use in the first and last sexual intercourse and of contraceptive methods, and a smaller mean number of partners, even after adjustments for sex and age. Conclusion: The greater the parental supervision, the better the sexual behavior for both sexes, although supervision seems to occur differently between girls and boys. These findings point to the role of the family in providing adolescents with monitoring, along with dialogue and affection, conditions that encourage healthy and risk-free sexual behavior.

ABSTRACT Objective: To analyze the association between self-reported sexual orientation and violence in the Brazilian population. Methods: This cross-sectional epidemiological study used the 2019 National Survey of Health database. Total violence and its subtypes (psychological, physical, and sexual) were analyzed in the previous 12 months. Prevalence and odds ratio adjusted for age group were estimated, with their respective 95% confidence intervals, according to the self-reported sexual orientation of the Brazilian population aged 18 years and older. Statistical significance was set at 5%. Results: Most of the Brazilian population self-identified as heterosexual (94.75%) and 1.89% as LGB+. This percentage was lower than that of respondents who refused to answer the question (2.28%). The prevalence of violence in the general population of Brazil was 18.27%, and the most common subtype was psychological violence (17.36%). The LGB+ population was more than twice as likely to experience any type of violence. LGB+ women had the highest prevalence in all violence subtypes, and heterosexual men had the lowest. LGB+ women were over three times more likely to experience physical violence compared to heterosexual ones. Meanwhile, the probability of LGB+ men experiencing sexual violence was almost eight times higher than in heterosexual men. Conclusion: The prevalence of violence against the LGB+ population was high in the country. Public policies aimed at this population are necessary to fight discrimination against sexual diversity and ensure the rights of non-heterosexual people.

Introducción: El síndrome de hiper-IgM es un error innato de la inmunidad, caracterizado por un defecto en el cambio de isotipo de inmunoglobulina, con valores disminuidos de IgG, IgA e IgE, y concentraciones normales o elevadas de IgM. Predispone a procesos infecciosos en el sistema respiratorio y aparato gastrointestinal, además de enfermedades autoinmunes y neoplasias. Reporte de caso: Paciente pediátrico de género masculino, de 5 años y 7 meses de edad, con antecedente de dos cuadros de neumonía (uno de estos grave) y diarrea crónica desde los 2 años. Neutropenia moderada persistente, disminución de la concentración de IgG y elevación de IgM. La citometría de flujo confirmó la ausencia de CD40L. Durante la evolución clínica tuvo afectación hepática temprana. Conclusión: El síndrome de hiper-IgM predispone a daño hepático, por lo que se requiere la evaluación completa y el diagnóstico oportuno. El tratamiento antiinfeccioso activo y el control de la respuesta inflamatoria son factores decisivos para establecer el tratamiento del daño hepático.

ObjectivesTo assess the ABC2-SPH score in predicting COVID-19 in-hospital mortality, during intensive care unit (ICU) admission, and to compare its performance with other scores (SOFA, SAPS-3, NEWS2, 4C Mortality Score, SOARS, CURB-65, modified CHA2DS2-VASc, and a novel severity score).Materials and methodsConsecutive patients (≥ 18 years) with laboratory-confirmed COVID-19 admitted to ICUs of 25 hospitals, located in 17 Brazilian cities, from October 2020 to March 2022, were included. Overall performance of the scores was evaluated using the Brier score. ABC2-SPH was used as the reference score, and comparisons between ABC2-SPH and the other scores were performed by using the Bonferroni method of correction. The primary outcome was in-hospital mortality.ResultsABC2-SPH had an area under the curve of 0.716 (95% CI 0.693–0.738), significantly higher than CURB-65, SOFA, NEWS2, SOARS, and modified CHA2DS2-VASc scores. There was no statistically significant difference between ABC2-SPH and SAPS-3, 4C Mortality Score, and the novel severity score.ConclusionABC2-SPH was superior to other risk scores, but it still did not demonstrate an excellent predictive ability for mortality in critically ill COVID-19 patients. Our results indicate the need to develop a new score, for this subset of patients.

A presente tese tem como tema central o processo de modificações do trabalho docente nas escolas públicas estaduais, a partir da implementação da reforma do ensino médio (REM) em curso no Brasil. Em face disso, procuramos compreender as relações entre um conjunto de documentos, que, alinhados e complementares à Lei nº. 13.415/2017, repercutiram nas condições de realização desse trabalho, instituindo novas formas de expropriação de direitos dos (as) professores (as) das escolas públicas estaduais. As mudanças vinculadas a esse processo se relacionam e vêm se desenvolvendo, intensificando e se revelando como parte de uma tendência que parece se constituir a partir de uma multiplicidade de causas, processos e aspectos vinculados a modificações no mundo do trabalho no século XXI (ANTUNES, 2018).

Abstract Lagoa do São Bento is a coastal restinga wetland, remnant of a paleolake, located in Maricá, RJ. The study aimed to inventory native aquatic plants, make a floristic comparison with other coastal lagoons and analyze the regeneration of this community after anthropic impacts. In the floristic analysis of aquatic plants, 45 species were listed, 39 of which are Angiosperms and six are Ferns. Aeschynomene paniculata, Hymenachne amplexicaulis, Montrichardia linifera and Torenia thouarsii are new records for the municipality and Tabebuia cassinoides is threatened. Emerging plants (21 spp.) stand out, followed by amphibians (16 spp.), free floating (4 spp.), fixed floating (3 spp.) and tolerant (1 sp.). The comparison showed greater similarity with the Jacarepiá lagoon, in Saquarema, sharing 21 species. The phytosociological inventory listed 87 species, 83 of which were Angiosperms and four Ferns. The species with the highest IVI were: Fuirena umbellata, Pleroma gaudichaudianum, Xyris jupicai, Typha domingensis, Salvinia aff. auriculata, Eleocharis interstincta, Rhynchospora gigantea, Nymphoides humboldtiana, Nymphaea caerulea and Clitoria laurifolia. Shannon, Simpson and equity indexes were 3.83, 0.03 and 0.85, respectively. Native aquatic species represent the majority of the total inventoried (54%), with a considerable supply of exotic and ruderal, whose colonization reflects the anthropic interventions.

This paper aims to analyze the intellectual structure and research fronts in application information security in smart cities to identify research boundaries, trends, and new opportunities in the area. It applies bibliometric analyses to identify the main authors and their influences on information security and the smart city area. Moreover, this analysis focuses on journals indexed in Scopus databases. The results indicate that there is an opportunity for further advances in the adoption of information security policies in government institutions. Moreover, the production indicators presented herein are useful for the planning and implementation of information security policies and the knowledge of the scientific community about smart cities. The bibliometric analysis provides support for the visualization of the leading research technical collaboration networks among authors, co-authors, countries, and research areas. The methodology offers a broader view of the application information security in smart city areas and makes it possible to assist new research that may contribute to further advances. The smart cities topic has been receiving much attention in recent years, but to the best of our knowledge, there is no research on reporting new possibilities for advances. Therefore, this article may contribute to an emerging body of literature that explores the nature of application information security and smart cities research productivity to assist researchers in better understanding the current emerging of the area.

Foodborne Diseases (FBDs) are a worldwide problem and occur after contaminated food has been ingested, signaling a lack of food quality. Even though the SARS-CoV-2 virus is not transmitted through food, the COVID-19 pandemic has caused several challenges worldwide that have had direct implications on food production and handling, stimulating and reinforcing the adoption of good manufacturing and food handling practices. The aim of this study was to analyze data on notifications of FBD in Brazil in the years before (2018 and 2019) and during (2020 and 2021) the COVID-19 pandemic. Secondary data from the National System of Notifiable Diseases was analyzed, evaluating: overall incidence rate, lethality and mortality, contamination sites, and criteria for confirming the etiological agent. There were 2206 records of FBDs, and the mortality rate was 0.5% in both periods. The incidence rate before the pandemic was 6.48 and during the pandemic was 3.92, while the mortality coefficient was 0.033 before and 0.019 during the pandemic, both per 100,000 inhabitants. There was no significant difference in the number of FBD notifications in the evaluated periods. There was a migration of the location of FBD, with a significant increase in FBD notifications in hospitals and health units and a reduction in notifications from social events. There was a significant increase in the type of criteria used to confirm outbreaks, with an increase in clinical laboratory tests and clinical reports for bromatology. The increase in notifications in hospitals and health units demonstrates the necessity of improving food safety knowledge and the attitudes and practices of food handlers and healthcare professionals.

This study investigates the internal architecture of Asian hornet nests (AHNs) using advanced imaging techniques, such as CT scanning and X-ray radiography, to understand their construction and function. The primary objective and significance of this study centre on drawing inspiration from the creative way Asian hornets construct their nests, with a particular focus on the architecture, design, functionality, and building materials of these nests. The architectural principles governing the construction of these nests, such as the arrangement of hexagonal cells, pedicels for load bearing, and adhesive materials, serve as a source of inspiration for innovative and sustainable design practices. The pedicels in Asian hornet nests play a crucial role in transferring load and ensuring stability. Additionally, AHNs’ adhesion to tree branches is essential for preventing collapse, and the pedicels provide necessary structural support. The knowledge gained from studying AHNs’ internal architecture could be applied directly to the architecture and civil engineering fields to improve structure stability and durability. The microstructure analysis of the paper-like material that hornets produce to build their nests indicates a complex and heterogeneous structure, composed of various plant fragments and fibres. This unique composition creates intricate grooves and pores, which are essential for regulating temperature and humidity levels within the outer envelope of the nest. The study of Asian hornet nests’ internal structure demonstrated that nature’s engineering principles inspire the design of durable and resilient structures in the construction industry. Civil engineers can incorporate similar principles into their designs to enhance the structural integrity and performance of buildings, bridges, and other infrastructure.

This article proposes a solution to the Profit-Based Unit Commitment (PBUC) problem to maximize the profit of a power generation company that owns thermal units and compressed air energy storage (CAES) systems, considering the Day-Ahead market. The proposed methodology is more realistic as it considers a mixed-integer nonlinear formulation of the PBUC. The problem is solved through two stages, with Stage 1 dedicated to obtaining the operational state of the generating units (On or Off) and the operation mode of the storage system (energy exchange: charging, discharging, idle). Stage 2 determines the dispatch of power from the thermoelectric units and the energy exchange in the storage system. The analysis of the system consisting of 20 thermoelectric units and three storage systems shows the efficiency of the proposed method in making decisions for the power generation company and is therefore promising for real-world applications.

Testing shear-resisting plates in steel connections is one of the most challenging laboratory undertakings in steel construction, as the most common experimental layout design includes simulating the connection with its adjoining members. This significant hindrance gained particular magnitude as the need to test prototypes of topologically optimised shear cover plates became more pressing. Indeed, new code-compliant topology optimisation approaches for steel construction have recently been offered, and physically non-linear analyses have been demonstrated to be vital for assessing these elements. Hence, a rapid and reliable experimental process has become a fundamental necessity. To answer this need, a novel layout is herein proposed, in which topologically optimised and previously numerically examined bolted shear plates of a well-known steel joint were tested. The results allowed for the definition of the material trilinear model for use in subsequent numerical analysis, as well as the validation of the numerical simulation results. The discrepancy between the previously mathematically anticipated and empirically determined ultimate resistance did not exceed 1.7%.

This work proposes a methodology based on multicriteria decision aid (MCDA) and a cluster analysis to identify ideal locations for the installation of police facilities or vehicle parking and policing around stadiums in Recife, Brazil, during potential violent sports events (criminal occurrences from football supporters or fanbases). A K-means unsupervised clustering algorithm is used to group criminal data into homogeneous clusters based on their characteristics. Each type of criminal occurrence is linked to a single cluster. The optimal location is addressed based on the PROMETHEE method (Preference Ranking Organization Method for Enrichment Evaluation), allowing clusters to be organized into a hierarchy based on the number of facilities (N), the average distance (D) from the criminal occurrence to the associated cluster, and the coverage level (C), which is the proportion of crime occurring in a location less than 500 m from the associated cluster. Through a data analysis of crimes and violence in the region, this study seeks to identify patterns of criminal behaviour and high-risk areas to determine the most strategic locations for police units and enhance the public security decision-making process. The choice for the k parameters ranged from 1 to 30, incorporating all regions of the analysis, with a computational cost of 43 min of running time using an Intel Core i3-3217U (1800 GHz and 10 GB of RAM). This approach and methodology can be useful for supporting public security policies in the region and can contribute to reducing violence around stadiums. The empirical application can help guide public managers’ decisions regarding resource allocation and the implementation of more effective security policies, with the aim of ensuring a safer environment for fans and residents in the areas near stadiums.

In real-world scenarios, deviations in the frequency response of instrumentation transformers can lead to distorted harmonic measurements, highlighting the critical role harmonic measurement plays in assessing power quality. The blind channel equalization technique offers a potential solution to improve the frequency response of a large number of instrumentation transformers already installed in substations. These transformers were designed to accurately measure only the fundamental phasor component. Therefore, in order to use them for harmonic phasor measurement, methodologies for reducing frequency distortion must be applied. In this work, we propose a novel approach to improve the frequency response of the instrument transformer using adaptive blind equalization. The blind technique can compensate for distortions caused by voltage and current transducers without requiring prior knowledge of input signals or circuit characteristics. The proposed methodology uses a Linear Prediction Filter to convert the colored noise present at the channel output into white noise. Furthermore, a genetic algorithm is used to find a pole to cancel possible zeroes present in the frequency response of some transducers. The main advantage of blind equalization with the genetic algorithm is its independence, operating without clear information about the channel or the input signal. Through extensive experimentation, we demonstrate the effectiveness of the proposed methodology in significantly reducing the absolute error in ratio and phase caused by current and voltage transformers. Simulated and laboratory experiments are presented in this paper.

The aetiology of anorexia nervosa (AN) presents a puzzle for researchers. Recent research has sought to understand the behavioural and neural mechanisms of these patients’ persistent choice of calorie restriction. This scoping review aims to map the literature on the contribution of habit-based learning to food restriction in AN. PRISMA-ScR guidelines were adopted. The search strategy was applied to seven databases and to grey literature. A total of 35 studies were included in this review. The results indicate that the habit-based learning model has gained substantial attention in current research, employing neuroimaging methods, scales, and behavioural techniques. Food choices were strongly associated with dorsal striatum activity, and habitual food restriction based on the self-report restriction index was associated with clinical impairment in people chronically ill with restricting AN. High-frequency repetitive transcranial magnetic stimulation (HF-rTMS) and Regulating Emotions and Changing Habits (REaCH) have emerged as potential treatments. Future research should employ longitudinal studies to investigate the time required for habit-based learning and analyse how developmental status, such as adolescence, influences the role of habits in the progression and severity of diet-related illnesses. Ultimately, seeking effective strategies to modify persistent dietary restrictions controlled by habits remains essential.

COVID-19 is an infectious disease caused by coronavirus 2 of the severe acute syndrome (SARS-CoV-2). Single nucleotide polymorphisms (SNPs) in genes, such as TLR2, responsible for an effective human immune response, can change the course of infection. The objective of this article was to verify associations between epidemiological factors and TLR2 SNP rs3804100 (Thymine [T] > Cytosine [C]) in professionals from Health Institutions (HI) who worked during the first pandemic wave and COVID-19. A case-control study was conducted with Belém-PA HI workers (Northern Brazil), divided into symptomatology groups (Asymptomatic-AS; n = 91; and Symptomatic-SI; n = 123); and severity groups classified by Chest Computerized Tomography data (symptomatic with pulmonary involvement—SCP; n = 35; symptomatic without pulmonary involvement—SSP; n = 8). Genotyping was performed by Sanger sequencing, and Statistical Analysis was conducted through the SPSS program. Bioinformatics servers predicted the biological functions of the TLR2 SNP. There were associations between the presence of comorbidities and poor prognosis of COVID-19 (especially between symptomatology and severity of COVID-19 and overweight and obesity) and between the sickness in family members and kinship (related to blood relatives). The homozygous recessive (C/C) genotype was not found, and the frequency of the mutant allele (C) was less than 10% in the cohort. No significant associations were found for this SNP in this cohort. The presence of SNP was indicated to be benign and causes a decrease in the stability of the TLR2 protein. These data can help the scientific community and medicine find new forms of COVID-19 containment.

ABSTRACT The Cerrado represents the largest extension of savanna in South America. It occupies large stretches of central Brazil, being fragmented towards the Northeast, Southeast, and South regions of the country. Examples of disjunct patches of vegetation with savanna physiognomy within the Caatinga can be found in the Chapada Diamantina, the Chapada do Araripe, in small areas of southern Ceará State, and also in the coastal plains. This study recorded the floristic composition of four savannas within the Caatinga in northern Ceará State and evaluated the floristic relationships between these and other savannas, Cerrado and Caatinga sites. Periodic floristic collections recorded 247 species distributed among 162 genera and 55 families. Fifty-seven percent of the recorded species were of herbaceous or sub-shrubby habit, while the majority of the flora was of the therophytic life-form. Biogeographic analyses revealed that the study sites differ from typical Cerrado in flora and life-form spectra and have closer floristic relationships with Caatinga vegetation. The presence of floristic elements from the Cerrado, together with species from the Caatinga, in the study sites allows us to conclude that these savanna enclaves in the Caatinga are composed of a mixed flora with typical elements of these two Brazilian biomes.

A misincentive is characterized as an incentive producing the opposite effect of motivating or preventing some specific action. In some circumstances, contract penalties such as sanctions and fines on late delivery or low-quality software encourage the irregularities instead of preventing them from occurring. The present study models misincentive behaviors in Information Technology Outsourcing (ITO) transactions as a Principal-Agent problem. Considering the non-linear relationship in software vendors’ cost structure and the client’s sensitivity to the product/service quality and delivery time, we offer theoretical modeling elucidating the best responses on agreements under incomplete and asymmetric information. Some empirical evidence of contractual misincentives is reported in outsourcing arrangements between private institutions and public administrations in Portugal. The contracts were divided into three categories: pure contracts with no incentive clauses, contracts with no explicit monetary penalties, and contracts under explicit sanctions. The results suggest that the knowledge of the penalty reduces the Agent’s uncertainty about the Principal’s cost structure and might lead them to intentionally delay the delivery of the technology.

This systematic review study analyzed the evidence of validity of the Dyadic Coping Inventory’s internal structure. This instrument measures the quality of communication and the strategies of marital coping in face of the stress experienced. Stress can affect the stability and maintenance of marital relationships depending on how it is faced by the couple. The measure is a reduced version of the Dyadic Coping Questionnaire and has been applied to various cultural contexts. This research study was registered at the International Pros­pective Register of Systematic Reviews database. Searches for publications were made on the Capes' Portal Periódico and Google Scholar, not refining the results. Two independent researchers selected, extracted, and evaluated the data. For the validity analysis of the internal structure, the Standards for Education and psychological Testing were consulted. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement was used to summarize the review. Of the 722 studies found, 15 were selected, from the Scopus, Web of Science, John Wiley & Sons, Directory of Open Access Journals, DergiPark, Pubmed and Taylor & Francis Online databases. All articles included were quantitative, cross-sectional and peer reviewed studies, mostly carried out in Europe and in 14 different languages. Despite variations in the number of factors and items, the instrument has shown to be stable and invariant across genders and cultures. These results suggested a reliable score to measure Dyadic Coping. This review can contribute to clinical practice and research in marital and family relationships.

ABSTRACT This retrospective case series study describes the clinical and vascular ultrasound findings of 26 dogs diagnosed with abdominal thrombosis. Images were selected based on the detection of intravascular echogenic thrombus or the absence of vascular flow on color Doppler, confirmed by surgery or necropsy. Images were acquired using the Mylab 40 model, with linear and microconvex multifrequency probes. All the reports were evaluated along with the corresponding images by a veterinary diagnostic imaging radiologist. The ultrasonographic aspects evaluated were echogenicity (92.3%), anechogenicity (7.7%), vascularization (11.5%), mineralization (15.4%), and recanalization (7.7%) of the thrombosis. The vascular and hemodynamic findings were dilation of the affected vein (57.7%), total occlusion of blood flow (30.8%), presence of turbulent flow (65.38%), and visualization of smoke signal (blood flow detected as moving echogenic points in dynamic bidimensional mode) (11.5%). Neoplasms (19 cases) and nephropathies (13 cases) were the most common clinical conditions in the affected dogs. Eleven cases of vascular invasion due to adrenal neoplasms were identified. The results indicate that the vascular ultrasound examination is an important method for diagnosis, as 23 of the 26 cases did not show any clinical signs of thrombosis.

This study examines the mediating effect of resilience on the relationship between work stress and job satisfaction. It is quantitative research with a sample of 418 workers from companies that operate in Brazil, analyzed through structural equation modeling and permutation analysis. The results show that resilience contributes to reducing the impacts of job stress on job satisfaction, indicating that the development and improvement of resilience should be encouraged in organizations so that employees can face adversity. Regarding the differences between millennials (up to 29 years old) and post-millennials (over 29 years old), the evidence shows that older employees can be more resilient; they deal better with colleagues and challenging situations and unexpected and accept challenges that contribute to professional growth. This study’s main theoretical contribution is the development of quantitative empirical research that relates labor stress and job satisfaction (consequent) influenced by resilience in a parsimonious model, not yet considered in the literature.