Search

Your search keyword '"Nakajima, Masahiro"' showing total 1,301 results
Start Over Author "Nakajima, Masahiro"
1,301 results on '"Nakajima, Masahiro"'

151. A Replication Study for the Association of rs11190870 With Curve Severity in Adolescent Idiopathic Scoliosis in Japanese

Author

Takahashi, Yohei, primary, Kou, Ikuyo, additional, Ogura, Yoji, additional, Miyake, Atsushi, additional, Takeda, Kazuki, additional, Nakajima, Masahiro, additional, Minami, Shohei, additional, Kawakami, Noriaki, additional, Uno, Koki, additional, Ito, Manabu, additional, Yonezawa, Ikuho, additional, Kaito, Takashi, additional, Yanagida, Haruhisa, additional, Watanabe, Kei, additional, Taneichi, Hiroshi, additional, Harimaya, Katsumi, additional, Taniguchi, Yuki, additional, Kotani, Toshiaki, additional, Tsuji, Taichi, additional, Suzuki, Teppei, additional, Sudo, Hideki, additional, Fujita, Nobuyuki, additional, Yagi, Mitsuru, additional, Chiba, Kazuhiro, additional, Kono, Katsuki, additional, Sakuma, Tsuyoshi, additional, Akazawa, Tsutomu, additional, Nishida, Kotaro, additional, Kakutani, Kenichiro, additional, Shigematsu, Hideki, additional, Iida, Takahiro, additional, Demura, Satoru, additional, Hosogane, Naobumi, additional, Okada, Eijiro, additional, Nakamura, Masaya, additional, Matsumoto, Morio, additional, Watanabe, Kota, additional, and Ikegawa, Shiro, additional

Published
2018
Full Text
View/download PDF

154. Su1317 - Development of a New Device to Detect Chemotaxis of Caenorhabditis Elegans using Capacitance Sensor for Pancreatic Cancer Screening

Author

Ishikawa, Takuya, primary, Hirooka, Yoshiki, additional, Kawashima, Hiroki, additional, Ohno, Eizaburo, additional, Suhara, Hiroki, additional, Matsushita, Masanobu, additional, Yamamura, Takeshi, additional, Furukawa, Kazuhiro, additional, Funasaka, Kohei, additional, Nakamura, Masanao, additional, Miyahara, Ryoji, additional, Watanabe, Osamu, additional, Fukuda, Toshio, additional, Ichikawa, Akihiko, additional, Nakajima, Masahiro, additional, Baba, Yuya, additional, and Goto, Hidemi, additional

Published
2018
Full Text
View/download PDF

157. Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes

Author

Nakagawa, Yosuke, Nakamura, Sou, Nakajima, Masahiro, Endo, Hiroshi, Dohda, Takeaki, Takayama, Naoya, Nakauchi, Hiromitsu, Arai, Fumihito, Fukuda, Toshio, and Eto, Koji

Subjects
platelet, megakaryocyte, bioreactor, flow, device, shear stress
Abstract

Induced pluripotent stem cell (iPSC) technology enables us to investigate various potential iPSC-based therapies. Although the safety of iPSC derivation has not been completely validated, anucleate cells, such as platelets or erythrocytes, derived from iPSCs are promising targets. However, the efficiency of invitro platelet generation from megakaryocytes (MKs) under static culture conditions is lower than is seen invivo. In this study, we demonstrate the proof of concept by a two-dimensional flow culture system that enabled us to increase platelet yield from human embryonic stem cell or iPSC-derived MKs using a biomimetic artificial blood vessel system. The bioreactor was composed of biodegradable scaffolds with ordered arrays of pores made to mimic invivo bone marrow through salt leaching. Within the system, two flows in different directions in which the angle between the directions of flow is 60 degrees but not 90 degrees contributed to suitable pressure and shear stress applied to MKs to promote platelet generation. Generated platelets derived from human embryonic stem cells or human induced pluripotent stem cells through the bioreactor with a 60-degree angle revealed intact integrin αIIbβ3 activation after agonist stimulation. Collectively, our findings indicate that two flows in different directions of two-dimensional flow culture may be a feasible system for invitro generation of platelets from pluripotent stem cells (i.e., iPSC-derived MKs) in numbers sufficient for transfusion therapy.

Published
2013

158. 「きぼう」曝露系システムの開発成果: 船外実験プラットフォーム

Author

Oikawa, Koki, Ozawa, Daisaku, Murakami, Atsushi, Takada, Masaharu, Nakajima, Masahiro, Yoshikawa, Masakazu, and Nakahara, Satoru

Abstract

形態: カラー図版あり, Physical characteristics: Original contains color illustrations, Translation title: Results of development for KIBO exposed system: JEM exposed facility, 資料番号: AA0061925015, レポート番号: JAXA-SP-12-015

Published
2013

159. Summary of Demonstration Mission for JEM Small Satellite Orbital Deployer

Author

Takata, Masaharu, Yoshie, Yuuki, Nakajima, Masahiro, Matsumura, Yuusuke, Doi, Shinobu, and Suzuki, Kazuya

Subjects
JEM Small Satellite Orbital Deployer (J-SSOD), Japan Experiment Module(JEM), Cubesat
Abstract

JEM Small Satellite Orbital Deployer (J-SSOD) is the small satellite launcher, which is capability of deploying CubeSats, 30-50cm class satellites, into the space from the International Space Station (ISS).This paper shows the explanation of the system and specifications for the J-SSOD, and results of the demonstrationmission for the J-SSOD., 資料番号: PA1410068000

Published
2013

161. Nanofabrication, nanoinstrumentation and nanoassembly by nanorobotic manipulation

Author

Fukuda, Toshio, Nakajima, Masahiro, Liu, Pou, and ElShimy, Haitham

Subjects
Nanotechnology -- Research, Robotics -- Research, Electron microscopes -- Usage, Nanotubes -- Product development, Nanotubes -- Structure, Computers and office automation industries, Engineering and manufacturing industries
Published
2009

162. Identification of lacto-N-biose I phosphorylase from Vibrio vulnificus CMCP6

Author

Nakajima, Masahiro and Kitaoka, Motomitsu

Subjects
Galactose -- Chemical properties, Gene expression -- Analysis, Phosphorylation -- Analysis, Vibrio -- Genetic aspects, Vibrio -- Physiological aspects, Biological sciences
Abstract

The homologous gene is expressed from Vibrio vulnificus CMCP6 in Escherichia coli and the gen product is characterized in order to understand its roles in V. vulnificus. The discovery of a lacto-N-biose I (LNB)-specific [beta]-1,3-galactosyl-N-acetylhexosamine phosphorylase (GalGlyNAcP) has resulted in recategorization of bifidobacterial GalGlyNAcPs a galacto-N-biose/lacto-N-biose I phosphorylases.

Published
2008

163. Colorimetric Quantification of α-D-galactose 1-phosphate

Author

Nihira, Takanori, Nakajima, Masahiro, Inoue, Kousuke, Nishimoto, Mamoru, Nakai, Hiroyuki, and Kitaoka, Motomitsu

Subjects
α-D-ガラクトース 1-リン酸, α-D-galactose 1-phosphate, 比色定量, lacto-N-biose phosphorylase, colorimetric quantification, ラクト-N- ビオースホスホリラーゼ
Abstract

Colorimetric quantification of α-D-galactose 1-phosphate (Gal 1-P) was performed by adding UDP-glucose hexose-1-phosphate uridylyltransferase and UDP-D-glucose to the conventional α-D-glucose 1-phosphate assay usingphosphoglucomutase and glucose 6-phosphate dehydrogenase. This method is very specific to Gal 1-P and results in a linearcalibration curve. The method was applied to continuous monitoring of the lacto-N-biose phosphorylase reaction. By addingmutarotase, galactokinase, and ATP, the method can be utilized for the quantification of D-galactose., α -D- ガラクトース1- リン酸（Gal 1-P）の比色定量として、ガラクトース定量法にホスファターゼを組み合わせる方法が知られるが、ガラクトースおよびガラクトース含有オリゴ糖が混在する試料中でのGal 1-P の定量法としては不都合があった。そこで我々はガラクトース代謝系酵素群に着目し、Gal 1-P のみを検出およびGal 1-P とガラクトースを同時に検出する2つの比色定量法を開発した。各種濃度のGal 1-P に発色試薬（UDP- グルコース- ヘキソース-1- リン酸ウリジリルトランスフェラーゼ、ホスホグルコムターゼ、グルコース-6- リン酸デヒドロゲナーゼ、 NAD+ またはThio-NAD+、UDP- グルコース、グルコース1,6-ビスリン酸、MgCl2含有トリス緩衝液）を混合し、経時的に340 nm（NAD+）または400 nm（Thio-NAD+）の吸収を測定した結果、20分程度で平衡に達し吸光度はGal 1-P 濃度に比例した。また上記試薬にムタロターゼ、ガラクトキナーゼおよびATPを追加することにより、各種濃度のガラクトースに対し同様の結果が得られた。また加リン酸分解酵素ラクト-N- ビオースホスホリラーゼの反応によって生じるGal 1-P を、発色試薬中にて反応停止操作を伴なわず連続的にモニタリングした結果、算出された活性値は各酵素濃度に比例しており、本定量法を用いた活性測定法の妥当性・有用性が示された。

Published
2011

170. Chitin-deacetylase activity induces appressorium differentiation in the rice blast fungus Magnaporthe oryzae

Author

Kuroki, Misa, primary, Okauchi, Kana, additional, Yoshida, Sho, additional, Ohno, Yuko, additional, Murata, Sayaka, additional, Nakajima, Yuichi, additional, Nozaka, Akihito, additional, Tanaka, Nobukiyo, additional, Nakajima, Masahiro, additional, Taguchi, Hayao, additional, Saitoh, Ken-ichiro, additional, Teraoka, Tohru, additional, Narukawa, Megumi, additional, and Kamakura, Takashi, additional

Published
2017
Full Text
View/download PDF

184. Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family

Author

Abe, Koichi, primary, Nakajima, Masahiro, additional, Yamashita, Tetsuro, additional, Matsunaga, Hiroki, additional, Kamisuki, Shinji, additional, Nihira, Takanori, additional, Takahashi, Yuta, additional, Sugimoto, Naohisa, additional, Miyanaga, Akimasa, additional, Nakai, Hiroyuki, additional, Arakawa, Takatoshi, additional, Fushinobu, Shinya, additional, and Taguchi, Hayao, additional

Published
2017
Full Text
View/download PDF

186. Mechanistic insight into the substrate specificity of 1,2-β-oligoglucan phosphorylase from Lachnoclostridium phytofermentans

Author

Nakajima, Masahiro, primary, Tanaka, Nobukiyo, additional, Furukawa, Nayuta, additional, Nihira, Takanori, additional, Kodutsumi, Yuki, additional, Takahashi, Yuta, additional, Sugimoto, Naohisa, additional, Miyanaga, Akimasa, additional, Fushinobu, Shinya, additional, Taguchi, Hayao, additional, and Nakai, Hiroyuki, additional

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results